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SKU:AR0003
Size:10 mL (sufficient for coating 100-200 slides)
Form Supplied:Concentrated solution (10X)
Application:Poly-L-Lysine Solution is for use in adhering tissue sections to both plasticware and glass surfaces for immunohistochemistry. It is intended for use as an adhesive subbing solution for immunohistochemistry (IHC) and routine histologic staining preparations.
Note: It has not been certified for cell culture.
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Overview

Product Name Poly-L-Lysine Solution
SKU/Catalog Number AR0003
Form Supplied Concentrated solution (10X)
Physical State Liquid
Storage Upon receipt store at 4°C. Poly-L-Lysine Solution is stable at 4°C for 6 months
The diluted working solution is stable at 4°C for 3 months.
Discard solutions if turbidity or bacterial growth develops.
Pack Size 10 mL (sufficient for coating 100-200 slides)
Content 0.1% (w/v) poly-L-lysine in deionized water with 0.01% thimerosal
Dilution Ratio1:10
EquivalentSanta Cruz (Product No. sc-286689), Millipore Sigma (Product No. P8920)
CAS No. 25988-63-0

Description

The loss of paraffin and frozen sections from slides has been a problem during routine histologic staining procedures. Various adhesives including albumin, gelatin, and chrome alum have been applied to slides to minimize this loss. Different solutions of poly-L-lysine have been shown to be most effective in promoting adhesion of sections. The polycationic nature of this molecule allows interaction with the anionic sites of tissue sections resulting in strong adhesive properties.

Background

α-Polylysine is commonly used to coat tissue cultureware as an attachment factor which improves cell adherence. This phenomenon is based on the interaction between the positively charged polymer and negatively charged cells or proteins. While the poly-L-lysine (PLL) precursor amino acid occurs naturally, the poly-D-lysine (PDL) precursor is an artificial product. The latter is therefore thought to be resistant to enzymatic degradation and so may prolong cell adherence.

Application

Poly-L-Lysine Solution is for use in adhering tissue sections to both plasticware and glass surfaces for immunohistochemistry. It is intended for use as an adhesive subbing solution for immunohistochemistry (IHC) and routine histologic staining preparations.
Note: It has not been certified for cell culture.

Important Product Information

• Use plastic containers and graduated cylinder when mixing or storing solution, and coating slides.

• Do not add fresh stock solution to used diluted working solution.

• Discard solutions if turbidity or bacterial growth develops.

• Slides must be clean before being coated.

Additional Materials Required

• Microscope slides

• Slide rack

• Plastic containers and graduated cylinder

• Drying oven (optional)

Procedure

1. Thaw Poly-L-Lysine solution at room temperature.

2. Dilute Poly-L-Lysine solution 1:10 with deionized water prior to coating slides.

3. Fully coat clean slides with diluted Poly-L-Lysine solution. A rack at a time and incubate for 5 minutes.

4. Aspirate the Poly-L-Lysine solution, drain slides and dry in 60°C oven for 1 hour or at room temperature overnight.

Poly-L-Lysine Solution Images

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Poly-L-Lysine Solution
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You Y, Huan P, Wang X, Liu B. Plos One. 2012;7(10):E47104. Doi: 10.1371/Journal.Pone.0047104. Epub 2012 Oct 9. The Potential Roles Of A Laminin Receptor In Adhesion And Apoptosis Of Cells Of The Marine Bivalve Meretrix Meretrix.
Du Y, Zhang J, Zheng Q, Li M, Liu Y, Zhang B, Liu B, Zhang H, Miao G. Neural Regen Res. 2014 Jun 1;9(11):1129-37. Doi: 10.4103/1673-5374.135315. Heavy Ion And X-Ray Irradiation Alter The Cytoskeleton And Cytomechanics Of Cortical Neurons.