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Breast Cancer Regulation
The loss of paraffin and frozen sections from slides has been a problem during routine histologic staining procedures. Various adhesives including albumin, gelatin, and chrome alum have been applied to slides to minimize this loss. Different solutions of poly-L-lysine have been shown to be most effective in promoting adhesion of sections. The polycationic nature of this molecule allows interaction with the anionic sites of tissue sections resulting in strong adhesive properties.
α-Polylysine is commonly used to coat tissue cultureware as an attachment factor which improves cell adherence. This phenomenon is based on the interaction between the positively charged polymer and negatively charged cells or proteins. While the poly-L-lysine (PLL) precursor amino acid occurs naturally, the poly-D-lysine (PDL) precursor is an artificial product. The latter is therefore thought to be resistant to enzymatic degradation and so may prolong cell adherence.
Poly-L-Lysine Solution is for use in adhering tissue sections to both plasticware and glass surfaces for immunohistochemistry. It is intended for use as an adhesive subbing solution for immunohistochemistry (IHC) and routine histologic staining preparations.
Note: It has not been certified for cell culture.
• Use plastic containers and graduated cylinder when mixing or storing solution, and coating slides.
• Do not add fresh stock solution to used diluted working solution.
• Discard solutions if turbidity or bacterial growth develops.
• Slides must be clean before being coated.
• Microscope slides
• Slide rack
• Plastic containers and graduated cylinder
• Drying oven (optional)
1. Thaw Poly-L-Lysine solution at room temperature.
2. Dilute Poly-L-Lysine solution 1:10 with deionized water prior to coating slides.
3. Fully coat clean slides with diluted Poly-L-Lysine solution. A rack at a time and incubate for 5 minutes.
4. Aspirate the Poly-L-Lysine solution, drain slides and dry in 60°C oven for 1 hour or at room temperature overnight.
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