POU3F2 Human Over-expression Lysates

Transient overexpression lysate of POU class 3 homeobox 2 (POU3F2)

Product Info Summary

SKU: LS005454
Size: 100 ug
Expression Host: HEK293T


Description: Transient overexpression lysate of POU class 3 homeobox 2 (POU3F2)

Product Name

POU3F2 Human Over-expression Lysates

SKU/Catalog Number



100 ug


Transient overexpression lysate of POU class 3 homeobox 2 (POU3F2)

Storage & Handling

Cite This Product

POU3F2 Human Over-expression Lysates (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS005454)



Detection Antibodies

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)


HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.

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