Anti-Interferon gamma/IFNG Antibody Picoband™ test

Rabbit lgG polyclonal antibody for Interferon gamma detection. Tested with WB,IHC-P in Human;Mouse;Rat, Cited in 4 publication(s).

Anti-Interferon gamma/IFNG Antibody Picoband™ test Info At a Glance

SKU: A00393-3
Size: 100ug/vial
Reactivity: Human,Mouse,Rat
Host: Rabbit
Application: IHC-P WB

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About Conjugates

HRP Conjugate Stabilizer - LifeXtend (ab270548) is a proprietary multi-component reagent system that protects antibody–HRP conjugates from instability, microbrial attack, and denaturation, thus ensuring the best possible performance in experiments performed at room temperature. The ability to store HRP conjugates at working dilutions eliminates waste and improves consistency from experiment to experiment.

Overview

Product Name

Anti-Interferon gamma/IFNG Antibody Picoband™

See more information about IFN-gamma, and browse related primary antibodies, ELISA kits and proteins

SKU/Catalog Number

A00393-3

Pack Size

100μg/vial

Storage & Handling

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Description

Rabbit IgG polyclonal antibody for Interferon gamma detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Cite This Product

Anti-Interferon gamma/IFNG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00393-3)

Antibodies Validation

Antibodies Validation Information

Product Specs

Host

Rabbit

Reactivity/Species

Human, Mouse, Rat

Applications

IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, wewill provide you a full credit to purchase Boster products.

Related Products

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

*Blocking peptide can be purchased at $50. Contact us for more information.

**Boster also offers various secondary antibodies or Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.

Immunogen

E. coli-derived human Interferon gamma recombinant protein (Position: Q24-G161).

Cross Reactivity

No cross reactivity with other proteins.

Innovator’s Reward

Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovator’s Reward

Assay Dilutions Overview

Our Boster Quality Guarantee for Anti-Interferon gamma/IFNG Antibody Picoband™ covers its use in the following applications.

Western blot, 0.1-0.5μg/ml

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Images & QC Assay Conditions

Figure 3. IHC analysis of IFNG using anti-IFNG antibody (A00393-3).

IFNG was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Figure 2. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

  • Lane 1: rat brain tissue lysates,
  • Lane 2: rat lung tissue lysates,
  • Lane 3: mouse brain tissue lysates,
  • Lane 4: mouse ovary tissue lysates,
  • Lane 5: mouse HEPA1-6 whole cell lysates.

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 1. Western blot analysis of IFNG using anti-IFNG antibody (A00393-3).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

  • Lane 1: human Hela whole cell lysates,
  • Lane 2: human placenta tissue lysates,
  • Lane 3: human U-87MG whole cell lysates,
  • Lane 4: human HL-60 whole cell lysates,
  • Lane 5: human U-937 whole cell lysates.

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFNG antigen affinity purified polyclonal antibody (Catalog # A00393-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFNG at approximately 19KD. The expected band size for IFNG is at 19KD.

Figure 6. IHC analysis of IFNG using anti-IFNG antibody (A00393-3).

IFNG was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IFNG Antibody (A00393-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Related products

Total Number Of Citations: 6

Total Number Of Citations: 4

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques.

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques.

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques.

Have you used Anti-Interferon Gamma/IFNG Antibody Picoband™?

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1-10 of 43 reviews

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Skin)

Detection

Heat mediated - Buffer/Enzyme Used: ph6 citrate

Results Summary

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting.

Dr. Bruce Colburn

Verified customer

Submitted Feb 23, 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Skin)

Detection

Heat mediated - Buffer/Enzyme Used: ph6 citrate

Results Summary

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting.

Dr. Bruce Colburn

Verified customer

Submitted Feb 23, 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Skin)

Detection

Heat mediated - Buffer/Enzyme Used: ph6 citrate

Results Summary

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting.

Dr. Bruce Colburn

Verified customer

Submitted Feb 23, 2020

1-10 of 43 reviews

2

2

Question

Could you recommend a Ki67 antibody (clone SP6) that would work in IHC-Fr using mouse tissue?

Dr. Bruce Colburn

Verified customer

Asked: Feb 23, 2020

2

Answer

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting.

Boster Scientific Support

Answered: Feb 23, 2020

2

2

Question

Could you recommend a Ki67 antibody (clone SP6) that would work in IHC-Fr using mouse tissue?

Dr. Bruce Colburn

Verified customer

Asked: Feb 23, 2020

2

Answer

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting.

Boster Scientific Support

Answered: Feb 23, 2020

2

2

Question

Could you recommend a Ki67 antibody (clone SP6) that would work in IHC-Fr using mouse tissue?

Dr. Bruce Colburn

Verified customer

Asked: Feb 23, 2020

2

Answer

Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting.

Boster Scientific Support

Answered: Feb 23, 2020

Gene/Protein Basic Information For IFNG (Source: Uniprot.Org, NCBI)

Uniprot Id

3458

NCBI Gene Id

100μg/vial

Species Of This Entry

Human

Gene Name

IFNG

Protein Name

Interferon Gamma

Superfamily

type II (or gamma) interferon family

Alternative Names

IFN-gamma|IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Molecular Weight

19348

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IFNG, check out the IFNG Infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IFNG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.

Take me to the IFNG Infographic

Conjugation

Sample size cannot be ordered with conjugation. Conjugated antibody size will be reduced to 50ug due to loss in the conjugation process.

Domestically in USA and Canada, ships next business day if available in stock.

Free Secondary Antibody Info

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IFNG Infographic

Card image

Comprehensive info on IFNG, beautifully presented.

Custom Antibodies

Did you know Boster offer custom antibody production service? You can have your antibodies custom made for as low as $600 if your research fit one of these 3 criteria:

  1. COVID-19 related;
  2. rare species;
  3. replacing discountinued antibodies.
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