Boster provides all the reagents you will need for performing your Western blotting SOP and more. See below for a detailed listing of our Western blot reagents and analysis kits sorted by the function/step they are used for in a typical western blot experiment protocol.

We provide a PDF for Western blotting protocols and troubleshooting tips. You can download it here

Solutions for western blotting workflow

All-In-One WB Kits Sample Preparation Total Protein Analysis
Electrophoresis/Gel Staining Protein Transfer Blocking Reagents
Chromogenic Development Chemiluminescent Development Ready-to-use Lysates

Check out the Boster technical newsletter archive which includes short blog articles for various experimental applications such as Western Blot, Immunohistochemistry (IHC), ELISA, and Flow Cytometry. These technical articles offer troubleshooting advice, protocol optimization tips, research pathway maps, and more! You can view archive here

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

SKU AR1149
Pack size 1L/pack
Applications Transfer buffer for Tris-glycine-sds gel
Availability: Ships next business day
SKU AR0135-04
Pack size 20 sheets/Pack
Pore Size0.25 µm
Availability: Ships next business day
SKU AR0135-02
Pack size 20 sheets/Pack
Pore Size0.25 µm

Availability: Ships in 5-7 business days

SKU AR0171
Pack size 1 kit (for 30 assays to stain the gel of 5 X8.5)
FormLiquid
Application To detect protein in polyacrylamide gels
Availability: Ships next business day
SKU AR1183
Product nameBroad Spectrum Phosphatase Inhibitor Cocktail
Pack size 1 mL
Applications WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments
Availability: Ships next business day
SKU AR1170
Pack size 200mL
Applications To detect antigen at low picogram level by reacting with horseradish peroxidase
Availability: Ships next business day
SKU AR1162
Pack size 100 mL
Form Liquid
Applications Resolving gel buffer for casting polyacrylamide gels
*Our Boster Guarantee covers the use of this product in the above tested applications.

Availability: Ships in 5-7 business days

SKU AR0101
Pack size 100 mL (100 assays for 0.1g of tissue)
Physical StateLiquid
Availability: Ships next business day
SKUMK1030
Pack size 1 kit (sufficient for staining100 slides)
ApplicationsISH

Availability: Ships in 5-7 business days

SKU AR0191
Pack size 20mL
FormLiquid

Availability: Ships in 5-7 business days

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