Boster provides all the reagents you will need for performing your Western blotting SOP and more. See below for a detailed listing of our Western blot reagents and analysis kits sorted by the function/step they are used for in a typical western blot experiment protocol.

We provide a PDF for Western blotting protocols and troubleshooting tips. You can download it here

Solutions for western blotting workflow

All-In-One WB Kits Sample Preparation Total Protein Analysis
Electrophoresis/Gel Staining Protein Transfer Blocking Reagents
Chromogenic Development Chemiluminescent Development Ready-to-use Lysates

Check out the Boster technical newsletter archive which includes short blog articles for various experimental applications such as Western Blot, Immunohistochemistry (IHC), ELISA, and Flow Cytometry. These technical articles offer troubleshooting advice, protocol optimization tips, research pathway maps, and more! You can view archive here

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

SKU AR1161
Pack size 500 mL
Form Solution (liquid)
Applications Protein separation
Sequencing gels

Availability: Ships in 5-7 business days

SKU AR1182-1
Pack size1mL
Physical StateLiquid, colorless, transparent
Description Boster’s Broad Spectrum Protease Inhibitor Cocktail (EDTA free) is a complex of various protease inhibitors, which has been tested for inhibiting proteases and esterase broadly. 
Availability: Ships next business day
SKU AR0056
Pack size 5 mL
Form Supplied1:100 Concentrated stock solution of Proteinase K in Tris-HCl buffer
Reagent Type Assistant Reagent
Availability: Ships next business day
SKU CR0001-25
Pack size50mL (sufficient reagents for 10000 cm2 of membrane)
DescriptionBoster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). 
Availability: Ships next business day
SKU CR0001-10
Pack size20mL (sufficient reagents for 4000 cm2 of membrane)
DescriptionBoster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram).

Availability: Ships in 5-7 business days

SKU CR0001-5
Pack size10mL (sufficient reagents for 2000 cm2 of membrane)
DescriptionBoster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). 

Availability: Ships in 5-7 business days

SKU AR1170
Pack size 200mL
Applications To detect antigen at low picogram level by reacting with horseradish peroxidase
Availability: Ships next business day
SKU AR1183
Product nameBroad Spectrum Phosphatase Inhibitor Cocktail
Pack size 1 mL
Applications WB sample preparation, protein purification, Co-IP, protein kinase activity assay experiments
Availability: Ships next business day
SKU AR0138
Pack size 1kit (Enough for 30-50 pieces of gel.)
Description Boster’s SDS-PAGE Gel Preparation Kit, Western Blotting Related Reagent.
Availability: Ships next business day
SKU AR0139
Pack size 1L/pack (for 5 assyas)
Form Dry-blend powder, sufficient to make 1L of Tris-Glycine SDS Running Buffer
Applications Running buffer for Tris-glycine gel electrophoresis
Availability: Ships next business day
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