Boster provides all the reagents you will need for performing your Western blotting SOP and more. See below for a detailed listing of our Western blot reagents and analysis kits sorted by the function/step they are used for in a typical western blot experiment protocol.

We provide a PDF for Western blotting protocols and troubleshooting tips. You can download it here

Solutions for western blotting workflow

All-In-One WB Kits Sample Preparation Total Protein Analysis
Electrophoresis/Gel Staining Protein Transfer Blocking Reagents
Chromogenic Development Chemiluminescent Development Ready-to-use Lysates

Check out the Boster technical newsletter archive which includes short blog articles for various experimental applications such as Western Blot, Immunohistochemistry (IHC), ELISA, and Flow Cytometry. These technical articles offer troubleshooting advice, protocol optimization tips, research pathway maps, and more! You can view archive here

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

SKUEK1003
Pack size 1 kit (for 1000 cm² membrane)
Applications Western blot
Availability: Ships next business day
SKUEK1002
Pack size 1 kit (for 1000 cm² membrane)
Applications Western blot
Availability: Ships next business day
SKU AR1023
Pack size 1 kit (20X)
Applications Immunohistochemistry
Availability: Ships next business day
SKU AR0157
Pack size 50mL (sufficient for lysis of 10g of bacterial biomass)
Form Liquid
Availability: Ships next business day
SKU AR0106
Pack size 1 kit
FormLiquid
Availability: Ships next business day
SKUAR1142
Pack size 1mL X 6 (sufficient to make 24mL of protein solution)
Description Boster’s Dual Color Protein Loading Buffer 4X is a complete solution for the preparation of protein samples prior to SDS-PAGE and monitoring protein transfer during Western blotting.
Availability: Ships next business day
SKU AR1112
Product nameSDS-PAGE Protein Loading Buffer 5X (Reducing)
Pack size 3mL
Form Liquid
Availability: Ships next business day
SKU AR0131
Pack size 6mL
FormLiquid

Availability: Ships in 5-7 business days

SKU AR1143
Pack size 6mL (6*1mL)
FormLiquid
Availability: Ships next business day
SKU AR1110
Pack size 1 kit
Applications Western blotting, protein expression assays, protein profiling and characterization, protein quantitation assays
Availability: Ships next business day
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