Boster provides all the reagents you will need for performing your Western blotting SOP and more. See below for a detailed listing of our Western blot reagents and analysis kits sorted by the function/step they are used for in a typical western blot experiment protocol.

We provide a PDF for Western blotting protocols and troubleshooting tips. You can download it here

Solutions for western blotting workflow

All-In-One WB Kits Sample Preparation Total Protein Analysis
Electrophoresis/Gel Staining Protein Transfer Blocking Reagents
Chromogenic Development Chemiluminescent Development Ready-to-use Lysates

Check out the Boster technical newsletter archive which includes short blog articles for various experimental applications such as Western Blot, Immunohistochemistry (IHC), ELISA, and Flow Cytometry. These technical articles offer troubleshooting advice, protocol optimization tips, research pathway maps, and more! You can view archive here

3 Guidelines for Using Secondary Antibodies

Apr 16, 2017

Keywords: secondary antibody, technical tips, troubleshooting, Flow cytometry antibodies

The purpose of using a secondary antibody is to amplify the primary antibody and antigen interactions signals. If you need secondary antibodies for your experiment, then you probably know there are many elements to consider when choosing the right antibody such as species reactivity. Dr. Booster has a few guidelines for you to keep in mind about secondary antibodies. Be sure to take a look and confirm everything is good to go.

SKUEK1005
Pack size 1 kit (for 1000 cm² membrane)
Applications Western blot

Availability: Ships in 7-10 business days

SKUEK1004
Pack size 1 kit (for 1000 cm² membrane)
Applications Western blot
Availability: Ships next business day
SKUEK1003
Pack size 1 kit (for 1000 cm² membrane)
Applications Western blot
Availability: Ships next business day
SKUEK1002
Pack size 1 kit (for 1000 cm² membrane)
Applications Western blot
Availability: Ships next business day
SKU AR0106
Pack size 1 kit
FormLiquid
Availability: Ships next business day
SKU AR0155
Pack size 1 kit
Availability: Ships next business day
SKU AR0138
Pack size 1kit (Enough for 30-50 pieces of gel.)
Description Boster’s SDS-PAGE Gel Preparation Kit, Western Blotting Related Reagent.
Availability: Ships next business day
SKU AR0139
Pack size 1L/pack (for 5 assyas)
Form Dry-blend powder, sufficient to make 1L of Tris-Glycine SDS Running Buffer
Applications Running buffer for Tris-glycine gel electrophoresis
Availability: Ships next business day
SKUAR1142
Pack size 1mL X 6 (sufficient to make 24mL of protein solution)
Description Boster’s Dual Color Protein Loading Buffer 4X is a complete solution for the preparation of protein samples prior to SDS-PAGE and monitoring protein transfer during Western blotting.

Availability: Ships in 7-10 business days

SKU AR1112
Product nameSDS-PAGE Protein Loading Buffer 5X (Reducing)
Pack size 3mL
Form Liquid
Availability: Ships next business day
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