Western Blotting Chromogenic/Chemiluminescent Development Products

antibody pairing

Introduction

The final step of Western blotting is detection; after the enzyme-linked secondary antibody has bound, adding the corresponding substrate causes a change in color or fluorescence that signals the presence of the target protein.

Procedure

After preparing the chromogenic or chemiluminescent substrate according to the instructions on the datasheet, cover the membrane with the substrate and incubate on a shaker until bands appear. Rinse the membrane and cover with film or a glass cover. Photograph the gel or expose the film and enjoy the excellent results of using Boster products.

For a detailed protocol for signal development, see our Western blot handbook.

Tips

Chemiluminescent development tends to be more sensitive, and more expensive than chromogenic development. Be sure to pick the right method for your needs.

SKU AR1170
Pack size 200mL
Applications To detect antigen at low picogram level by reacting with horseradish peroxidase
Availability: Ships next business day
SKU CR0001-5
Pack size10mL (sufficient reagents for 2000 cm2 of membrane)
DescriptionBoster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). 

Availability: Ships in 5-7 business days

SKU CR0001-10
Pack size20mL (sufficient reagents for 4000 cm2 of membrane)
DescriptionBoster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram).

Availability: Ships in 5-7 business days

SKU CR0001-25
Pack size50mL (sufficient reagents for 10000 cm2 of membrane)
DescriptionBoster’s Hypersensitive Western Blot Chemiluminescent Substrate is a luminol-based chemiluminescent substrate and it can detect horseradish peroxidase (HRP) at high sensitivity levels (low picogram). 
Availability: Ships next business day
SKU AR1196
Pack size 50mL
Applications To detect antigen at low-picogram to mid-femtogram level by reacting with horseradish peroxidase

Availability: Ships in 5-7 business days

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