SDS-Polyacrylamide Gel Electrophoresis Kits and Reagents

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Introduction

Once your protein sample has been extracted, prepared, and quantified, it is time to perform gel electrophoresis. With proper preparation and execution, gel electrophoresis will cleanly separate the proteins by size, allowing you to measure not only your protein’s presence, but it’s relative size as well. Boster provides SDS-PAGE Gel Preparation kits and other high-quality reagents so you can get the most out of your samples.

Procedure

To prepare your sample for electrophoresis, mix the sample with Boster dual color protein loading buffer and denature the resulting mixture in a 100°C water bath. Once your sample is ready, fill your electrophoresis chamber with enough Boster electrophoresis buffer to cover the gel. Carefully pipette your sample into the wells, using enough volume to ensure consistent amounts of protein, and making sure not to poke the pipette tip into the gel. Connect your electrophoresis chamber to the power supply of your choice, and run the gel for enough time to adequately separate your protein sample.

After your gel has run for an appropriate amount of time, us one of Boster’s protein visualization reagents to verify the success and quality of your electrophoresis experiment.

For a detailed protocol for gel electrophoresis, see our Western blot handbook.

Tips

  1. For better results, optimize the gel concentration, SDS concentration, and run time and temperature prior to running the gel
  2. Ensure that each well has the same amount of protein before running the gel
  3. If you encounter streaked, distorted, or smile bands, check your electrophoresis parameters. See our troubleshooting guides for more information
SKU AR0145
Product name Coomassie (Bradford) Protein Assay Kit(for 100-1500 μg/ml protein)
Form Solution (liquid)
Applications Western blotting, ELISA, immunofluorescence; protein expression assays, protein profiling and characterization; protein quantitation assays
Availability: Ships next business day
SKU AR0171
Pack size 1 kit (for 30 assays to stain the gel of 5 X8.5)
FormLiquid
Application To detect protein in polyacrylamide gels

Availability: Ships in 5-7 business days

SKU AR0170
Pack size100 mL(for 10 assays to stain gels of 5 x 8.5cm)
FormLiquid
ApplicationsFor protein staining on polyacrylamide gels.
Availability: Ships next business day
SKU AR0140
Pack size1kit (for 10 assays to stain gels of 5 x 8.5cm)
FormLiquid
ApplicationsFor protein staining on polyacrylamide gels.

Availability: Ships in 5-7 business days

SKU AR0138
Pack size 1kit (Enough for 30-50 pieces of gel.)
Description Boster’s SDS-PAGE Gel Preparation Kit, Western Blotting Related Reagent.
Availability: Ships next business day
SKU AR0139
Pack size 1L/pack (for 5 assyas)
Form Dry-blend powder, sufficient to make 1L of Tris-Glycine SDS Running Buffer
Applications Running buffer for Tris-glycine gel electrophoresis
Availability: Ships next business day
SKUAR1142
Pack size 1mL X 6 (sufficient to make 24mL of protein solution)
Description Boster’s Dual Color Protein Loading Buffer 4X is a complete solution for the preparation of protein samples prior to SDS-PAGE and monitoring protein transfer during Western blotting.
Availability: Ships next business day
SKU AR1112
Product nameSDS-PAGE Protein Loading Buffer 5X (Reducing)
Pack size 3mL
Form Liquid
Availability: Ships next business day
SKU AR0131
Pack size 6mL
FormLiquid
Availability: Ships next business day
SKU AR1143
Pack size 6mL (6*1mL)
FormLiquid
Availability: Ships next business day
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