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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Want to have all technical references by your finger tips? Download our FREE eBooks for WB, IHC, ELISA, Flow cytometry and Molecular biology here. These ebooks contain detailed information regarding the principles, protocols troubleshooting tips and optimization tips for their respective assays. Handy for newbies and veterans alike.
Breast Cancer Regulation
In order to visualize your target protein through antibody binding, the separated protein sample produced by gel electrophoresis must be transferred to a membrane. Using an electric field, the proteins in the PAGE gel can be pushed out of the gel matrix and onto a nitrocellulose membrane while maintaining their position in separate bands. This exposes them on the surface of the membrane, allowing effective incubation, washing, and substrate development, unimpeded by the matrix of the PAGE gel.
After soaking your membrane in methanol, immerse it in Boster transfer buffer until it become saturated and sinks. Assemble the transfer cassette from the bottom up, starting with the cathode plate, and building up in the following order: buffer-soaked Boster transfer pad, filter paper, gel, membrane, filter paper, Boster transfer pad, anode plate. Take great care to avoid trapping any bubbles between the layers, and avoid moving the membrane after it has contacted the gel.
Run the transfer setup for approximately 30 minutes at 25V.
For a detailed protocol for membrane transfer, see our Western blot handbook.
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