Protein Transfer



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  1. WB Stripping Buffer AR0153
    $60.00
    • Western Blotting Reagents, Protein Transfer
    • 100ml
    • boster_tick.pngShips next business day
    Boster’s WB Stripping Buffer is a ready-to-use solution for removing primary and secondary antibodies from probed Western blot membranes to allow chemiluminescent Western blots to be reprobed. Learn More
  2. Western Blotting Filter Paper, 9 cm × 7.5 cm AR0173
    $15.00
    • Western Blotting Reagents, Protein Transfer
    • 9 cm × 7.5 cm, 100 sheets
    • boster_tick.pngShips next business day
    Boster’s Western Blotting Filter Paper is pre-cut cotton fiber for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes. These filter papers are manufactured with ultrapure water and contain no additives that can interfere with any application. The smooth sheets are suitable for use with alcohol and other organic solvents involved in protein transfer and nucleic acid blotting. The papers also provide a uniform flow of buffer through the gel to the transfer membrance in a blotting sandwich. Learn More
  3. Western Blotting Filter Paper, 12.5 cm×12.5 cm AR0172
    $20.00
    • Western Blotting Reagents, Protein Transfer
    • 12.5 cm × 12.5 cm, 100 sheets
    • boster_tick.pngShips next business day
    Boster’s Western Blotting Filter Paper is pre-cut cotton fiber for wet or semi-dry, passive or electrophoretic transfer of proteins from polyacrylamide gels (SDS-PAGE) to PVDF, nitrocellulose, or other membranes. These filter papers are manufactured with ultrapure water and contain no additives that can interfere with any application. The smooth sheets are suitable for use with alcohol and other organic solvents involved in protein transfer and nucleic acid blotting. The papers also provide a uniform flow of buffer through the gel to the transfer membrance in a blotting sandwich. Learn More
  4. Tris-Glycine SDS Buffer Pack AR1149
    $5.00
    • Western Blotting Reagents, Protein Transfer
    • 1L
    • boster_tick.pngShips next business day
    Boster’s Tris-Glycine SDS Buffer Pack is a pouch of dry-blend powder that is sufficient to make 1L of standard transfer buffer for wet or semi-dry electrophoretic protein transfer from gel to blotting membrane. Learn More
  5. Nitrocellulose Membrane, 0.45 µm, 9 cm x 10cm AR0135-04
    $45.00
    • Western Blotting Reagents, Protein Transfer
    • 0.45um, 9cm × 10 cm; 20 sheets
    • boster_tick.pngShips next business day
    Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilize proteins, glycoproteins, or nucleic acids. Boster’s Nitrocellulose Membrane, 0.45 µm, 9 cm x 10cm is used for a wide range of protein molecular weights and nucleic acids >500 bp. Learn More
  6. Nitrocellulose Membrane, 0.25 µm, 9 cm x 10cm AR0135-02
    $45.00
    • Western Blotting Reagents, Protein Transfer
    • 0.22um, 9cm × 10 cm; 20 sheets
    • boster_tick.pngShips next business day
    Nitrocellulose membranes are a popular matrix used in protein blotting because of their high protein-binding affinity, compatibility with a variety of detection methods (chemiluminescence, chromogenic, and fluorescence), and the ability to immobilize proteins, glycoproteins, or nucleic acids. Boster’s Nitrocellulose Membrane, 0.25 µm, 9 cm x 10cm is used for low molecular–weight proteins and nucleic acids <500 bp. Learn More

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Western Blotting Transfer Products

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Introduction

In order to visualize your target protein through antibody binding, the separated protein sample produced by gel electrophoresis must be transferred to a membrane. Using an electric field, the proteins in the PAGE gel can be pushed out of the gel matrix and onto a nitrocellulose membrane while maintaining their position in separate bands. This exposes them on the surface of the membrane, allowing effective incubation, washing, and substrate development, unimpeded by the matrix of the PAGE gel.

Procedure

After soaking your membrane in methanol, immerse it in Boster transfer buffer until it become saturated and sinks. Assemble the transfer cassette from the bottom up, starting with the cathode plate, and building up in the following order: buffer-soaked Boster transfer pad, filter paper, gel, membrane, filter paper, Boster transfer pad, anode plate. Take great care to avoid trapping any bubbles between the layers, and avoid moving the membrane after it has contacted the gel.

Run the transfer setup for approximately 30 minutes at 25V.

For a detailed protocol for membrane transfer, see our Western blot handbook.

Tips

  1. Optimize the transfer type to get the best results for your application
  2. To avoid ghost bands, streaks, and splotches, take care to avoid trapped bubbles between the membrane and gel, and do not move the membrane after it has contacted the gel. See our troubleshooting guides for more information.