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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Want to have all technical references by your finger tips? Download our FREE eBooks for WB, IHC, ELISA, Flow cytometry and Molecular biology here. These ebooks contain detailed information regarding the principles, protocols troubleshooting tips and optimization tips for their respective assays. Handy for newbies and veterans alike.
Breast Cancer Regulation
Western blotting among the most frequently employed techniques used to detect proteins based on antigen-antibody interactions. The first step of western blotting is sample preparation; your protein of interest must be extracted from the tissue or cell culture it’s expressed in, protected from degradation, and prepared to be run on a gel. Boster provides products for sample preparation, including specialized extraction kits, lysis buffers, and protease inhibitors.
To extract a protein sample from a cell culture on solid medium, first rinse the culture with ice-cold PBS solution, then add ice-cold lysis buffer. Scrape adherent cells off the dish using a cold plastic scraper, or digest the cells with 0.05% trypsin. Once the cells are suspended, centrifuge the sample, draw off the supernatant fluid, and wash the pellet with ice cold PBS buffer. Re-suspend the cells in Boster mammalian cell protein extraction reagent and sonicate the solution for about 10-15 seconds, then lyse the cells with Boster RIPA lysis buffer and additional sonication. Centrifuge the sample to separate lipids and cellular debris from the soluble cell proteins, and aliquot the middle layer from the centrifuged sample into a fresh tube and store it at -20C.
The aliquots of cell lysis supernatant are ready for total protein quantification and gel electrophoresis.
For a detailed protocol of sample preparation starting with tissue samples, see our Western blot handbook.
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