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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
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The following guide serves as a checklist for the possible causes and solutions with respect to some of the most commonly encountered problems from molecular biology experimental techniques. We at Boster Bio are committed to helping our customers get better results. While the troubleshooting guide below covers a multitude of problems encountered while performing in the lab, we do not expect it to be the exclusive solution to any problems during your specific experiment. We hope that you will find the information beneficial to you and useful as a reference guide in troubleshooting any problems you may encounter. If you ever need more assistance with your experiments, please contact the Boster Support Team by email at firstname.lastname@example.org
Increase sample volume
Increase lysis time or add enzymatic lysis step
Increase lysis time in 10 min
Make sure that vortex and resuspension steps allow a good homogenization
Suspend DNA or RNA in less volume
Repeat extraction protocol from precipitation process
>>Did you know Boster has a convenient Miniprep Kit For Plasmid DNA Extraction And Purification?
Perform a temperature gradient PCR
Make new primer work solution
Increase template concentration
Decrease Tm temperature
Increase cDNA concentration
Check DNA template quality in Nanodrop
Verify time and temperature settings
Use new template
Increase Tm temperature
Avoid self-complementary sequences within primers
Avoid stretches of 4 or more of the same nucleotide or dinucleotide repeats
Lower primer concentration
Follow general rules of primer design
Decrease the number of cycles
Amplification in negative control
Use new reagents, namely buffer and polymerase
“Homemade” polymerases usually contain genetic contaminants. Try a commercial polymerase instead.
Make sure to use sterile tips
Low yields of PCR product
Increase number of cycles by 10
High quantification in standards
Use new diluted standards
Check pipettes calibration
High variability in replicates
Verify pipettes calibration
Use fresh diluted standards
Calibrate optics of the system
Repeat with rox-normalisation dye
Bands and smear are very intense
Reduce the number of cycles
Incorrect product size
Look for additional primer complementary sequences in the template
Make new primer work solutions
>>Did you know Boster has a convenient PCR Master Mix? Save your time and reduce contamination with our pre-mixed formulation!