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PicoKine®Rat Fractalkine PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl6-gcp2-picokine-trade-elisa-kit-ek0359-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0359.pngHuman CXCL6/GCP2 ELISA Kit PicoKine®Human CXCL6/GCP2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-g-csf-picokine-trade-elisa-kit-ek0360-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0360.pngHuman G-CSF ELISA Kit PicoKine®Human G-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-g-csf-picokine-trade-elisa-kit-ek0361-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0361.pngMouse G-CSF / CSF3 ELISA Kit PicoKine®Mouse G-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gdnf-picokine-trade-elisa-kit-ek0362-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0362.pngHuman GDNF / Glial Derived Neurotrophic Factor ELISA Kit PicoKine®Human GDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-gdnf-picokine-trade-elisa-kit-ek0363-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0363_2.pngRat GDNF ELISA Kit PicoKine®Rat GDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gm-csf-picokine-trade-elisa-kit-ek0364-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0364.pngHuman GM-CSF ELISA Kit PicoKine®Human GM-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-gm-csf-picokine-trade-elisa-kit-ek0365-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0365.pngMouse GM-CSF ELISA Kit PicoKine®Mouse GM-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-gm-csf-picokine-trade-elisa-kit-ek0366-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0366.pngRat GM-CSF ELISA Kit PicoKine®Rat GM-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gp130-il6st-picokine-trade-elisa-kit-ek0367-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0367.jpgHuman Gp130/IL6ST ELISA Kit PicoKine®Human Gp130/IL6ST PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hgf-picokine-trade-elisa-kit-ek0369-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0369.jpgHuman HGF/Hepatocyte growth factor ELISA Kit PicoKine®Human HGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-icam-1-picokine-trade-elisa-kit-ek0370-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0370.pngHuman ICAM-1 / CD54 ELISA Kit PicoKine®Human ICAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-icam-1-picokine-trade-elisa-kit-ek0371-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0371_1.pngMouse ICAM-1 / CD54 ELISA Kit PicoKine®Mouse ICAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-icam-1-picokine-trade-elisa-kit-ek0372-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0372.pngRat ICAM-1 ELISA Kit PicoKine®Rat ICAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ifn-gamma-picokine-trade-elisa-kit-ek0373-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0373.pngHuman IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®Human IFN gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-ifn-gamma-picokine-trade-elisa-kit-ek0374-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0374.pngRat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine®Rat IFN gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ifn-gamma-picokine-trade-elisa-kit-ek0375-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0375.pngMouse IFN Gamma / IFNG / Interferon gamma ELISA Kit PicoKine®Mouse IFN gamma PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-igf-1-picokine-trade-elisa-kit-ek0377-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0377.pngRat IGF-1 ELISA Kit PicoKine®Rat IGF-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-igf-1-picokine-trade-elisa-kit-ek0378-boster.html2026-03-24T05:02:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0378.pngMouse IGF-1 ELISA Kit PicoKine®Mouse IGF-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-igf-2-picokine-trade-elisa-kit-ek0380-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0380.pngRat IGF-2 ELISA Kit PicoKine®Rat IGF-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-igf-2-picokine-trade-elisa-kit-ek0381-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0381.pngMouse IGF-2 ELISA Kit PicoKine®Mouse IGF-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-igfbp-1-picokine-trade-elisa-kit-ek0382-boster.html2026-04-03T05:00:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0382.pngHuman IGFBP-1 ELISA Kit PicoKine®Human IGFBP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-igfbp-1-picokine-trade-elisa-kit-ek0383-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0383_1.pngMouse IGFBP-1 ELISA Kit PicoKine®Mouse IGFBP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-igfbp2-picokine-trade-elisa-kit-ek0384-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0384_1.pngHuman IGFBP2 ELISA Kit PicoKine®Human IGFBP2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-igfbp2-picokine-trade-elisa-kit-ek0385-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0385.jpgMouse IGFBP2 ELISA Kit PicoKine®Mouse IGFBP2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-igfbp-3-picokine-trade-elisa-kit-ek0386-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0386_1.jpgHuman IGFBP-3 ELISA Kit PicoKine®Human IGFBP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-igfbp-3-picokine-trade-elisa-kit-ek0387-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0387.pngMouse IGFBP-3 ELISA Kit PicoKine®Mouse IGFBP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-1-alpha-picokine-trade-elisa-kit-ek0389-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0389.jpgHuman IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®Human IL-1 alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-1-alpha-picokine-trade-elisa-kit-ek0390-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0390.jpgRat IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®Rat IL-1 alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-1-alpha-picokine-trade-elisa-kit-ek0391-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0391.jpgMouse IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine®Mouse IL-1 alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-1-beta-picokine-trade-elisa-kit-ek0392-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0392.jpgHuman IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®Human IL-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-1-beta-picokine-trade-elisa-kit-ek0393-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0393_2.pngRat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®Rat IL-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-1-beta-picokine-trade-elisa-kit-ek0394-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0394.jpgMouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®Mouse IL-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il1r1-picokine-trade-elisa-kit-ek0395-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0395.jpgHuman IL1R1/Il 1 Ri ELISA Kit PicoKine®Human IL1R1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il1r2-picokine-trade-elisa-kit-ek0396-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0396.pngHuman IL1R2/Il 1 Rii ELISA Kit PicoKine®Human IL1R2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-2-picokine-trade-elisa-kit-ek0397-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0397_1.pngHuman IL-2/Interleukin-2 ELISA Kit PicoKine®Human IL-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-2-picokine-trade-elisa-kit-ek0398-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0398.pngMouse IL-2 ELISA Kit PicoKine®Mouse IL-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-2-picokine-trade-elisa-kit-ek0399-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0399.pngRat IL-2/Interleukin-2 ELISA Kit PicoKine®Rat IL-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd25-il-2sr-alpha-picokine-trade-elisa-kit-ek0400-boster.html2026-03-24T05:02:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0400.jpgHuman CD25/IL-2sR Alpha ELISA Kit PicoKine®Human CD25/IL-2sR alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-3-picokine-trade-elisa-kit-ek0402-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0402.pngHuman IL-3 ELISA Kit PicoKine®Human IL-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-3-picokine-trade-elisa-kit-ek0403-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0403.jpgMouse IL-3 ELISA Kit PicoKine®Mouse IL-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-4-picokine-trade-elisa-kit-ek0404-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0404.pngHuman IL-4/Interleukin-4 ELISA Kit PicoKine®Human IL-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-4-picokine-trade-elisa-kit-ek0405-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0405.jpgMouse IL-4/Interleukin-4 ELISA Kit PicoKine®Mouse IL-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-4-picokine-trade-elisa-kit-ek0406-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0406.pngRat IL-4/Interleukin-4 ELISA Kit PicoKine®Rat IL-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-5-picokine-trade-elisa-kit-ek0407-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0407.jpgHuman IL-5/Interleukin-5 ELISA Kit PicoKine®Human IL-5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-5-picokine-trade-elisa-kit-ek0408-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0408.pngMouse IL-5/Interleukin-5 ELISA Kit PicoKine®Mouse IL-5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-6-picokine-trade-elisa-kit-ek0410-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0410.jpgHuman IL-6/Interleukin-6 ELISA Kit PicoKine®Human IL-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-6-picokine-trade-elisa-kit-ek0411-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0411.pngMouse IL-6/Interleukin-6 ELISA Kit PicoKine®Mouse IL-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-6-picokine-trade-elisa-kit-ek0412-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0412.pngRat IL-6/Interleukin-6 ELISA Kit PicoKine®Rat IL-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-8-picokine-trade-elisa-kit-ek0413-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0413.jpgHuman IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine®Human IL-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-10-picokine-trade-elisa-kit-ek0416-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0416.jpgHuman IL-10/Interleukin-10 ELISA Kit PicoKine®Human IL-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-10-picokine-trade-elisa-kit-ek0417-boster.html2026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0417.pngMouse IL-10/Interleukin-10 ELISA Kit PicoKine®Mouse IL-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-10-picokine-trade-elisa-kit-ek0418-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0418.pngRat IL-10/Interleukin-10 ELISA Kit PicoKine®Rat IL-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-11-picokine-trade-elisa-kit-ek0419-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0419.pngHuman IL-11/Interleukin-11 ELISA Kit PicoKine®Human IL-11 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-12-p70-picokine-trade-elisa-kit-ek0421-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0421.jpgHuman IL-12(p70) ELISA Kit PicoKine®Human IL-12(p70) PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-12-p70-picokine-trade-elisa-kit-ek0422-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0422_1.pngMouse IL-12(p70) ELISA Kit PicoKine®Mouse IL-12(p70) PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-13-picokine-trade-elisa-kit-ek0425-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0425.jpgMouse IL-13/Interleukin-13 ELISA Kit PicoKine®Mouse IL-13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-15-picokine-trade-elisa-kit-ek0426-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0426_1.pngHuman IL-15/Interleukin-15 ELISA Kit PicoKine®Human IL-15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-16-picokine-trade-elisa-kit-ek0428-boster.html2026-03-24T05:02:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0428.pngHuman IL-16 ELISA Kit PicoKine®Human IL-16 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-17-il-17a-picokine-trade-elisa-kit-ek0430-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0430_1.pngHuman IL-17/IL-17A ELISA Kit PicoKine®Human IL-17 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-17-il-17a-picokine-trade-elisa-kit-ek0431-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0431.pngMouse IL-17/IL-17A ELISA Kit PicoKine®Mouse IL-17 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-laminin-picokine-trade-elisa-kit-ek0434-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0434_1.pngHuman Laminin ELISA Kit PicoKine®Human Laminin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-laminin-picokine-trade-elisa-kit-ek0435-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0435.pngRat Laminin ELISA Kit PicoKine®Rat Laminin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-laminin-picokine-trade-elisa-kit-ek0436-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0436_2.pngMouse Laminin ELISA Kit PicoKine®Mouse Laminin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-leptin-picokine-trade-elisa-kit-ek0437-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0437.pngHuman Leptin ELISA Kit PicoKine®Human Leptin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-leptin-receptor-picokine-trade-elisa-kit-ek0440-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0440.pngMouse Leptin Receptor ELISA Kit PicoKine®Mouse Leptin receptor PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mcp-1-picokine-trade-elisa-kit-ek0441-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0441.jpgHuman MCP-1 / CCL2 ELISA Kit PicoKine®Human MCP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl8-mcp-2-picokine-trade-elisa-kit-ek0442-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0442_1.pngHuman CCL8/MCP-2 ELISA Kit PicoKine®Human CCL8/MCP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl7-mcp-3-picokine-trade-elisa-kit-ek0443-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0443_1.pngHuman CCL7/MCP-3 ELISA Kit PicoKine®Human CCL7/MCP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-m-csf-picokine-trade-elisa-kit-ek0444-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0444.jpgHuman M-CSF ELISA Kit PicoKine®Human M-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-m-csf-picokine-trade-elisa-kit-ek0445-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0445.pngMouse M-CSF ELISA Kit PicoKine®Mouse M-CSF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl22-mdc-picokine-trade-elisa-kit-ek0446-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0446.pngHuman CCL22/MDC ELISA Kit PicoKine®Human CCL22/MDC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl22-mdc-picokine-trade-elisa-kit-ek0447-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0447.pngMouse CCL22/MDC ELISA Kit PicoKine®Mouse CCL22/MDC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mip-1-alpha-picokine-trade-elisa-kit-ek0448-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0448_1.jpgHuman MIP-1Alpha/CCL3 ELISA Kit PicoKine®Human MIP-1 alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl3-mip1-alpha-picokine-trade-elisa-kit-ek0449-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0449.jpgMouse MIP-1Alpha/CCL3 ELISA Kit PicoKine®Mouse CCL3/MIP1 alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl4-mip-1-beta-picokine-trade-elisa-kit-ek0450-boster.html2026-03-24T05:02:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0450.jpgHuman CCL4/MIP-1 Beta ELISA Kit PicoKine®Human CCL4/MIP-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mip-2-picokine-trade-elisa-kit-ek0452-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0452.pngMouse MIP-2 ELISA Kit PicoKine®Mouse MIP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mip-3-alpha-ccl20-picokine-trade-elisa-kit-ek0453-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0453.jpgHuman MIP-3 Alpha / CCL20 / MIP3a ELISA Kit PicoKine®Human MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mip-3-alpha-ccl20-picokine-trade-elisa-kit-ek0454-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0454.jpgMouse MIP-3 Alpha/CCL20 ELISA Kit PicoKine®Mouse MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-mip-3-alpha-ccl20-picokine-trade-elisa-kit-ek0455-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0455_1.pngRat MIP-3 Alpha/CCL20 ELISA Kit PicoKine®Rat MIP-3 alpha/CCL20 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl19-mip-3-beta-picokine-trade-elisa-kit-ek0456-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0456.pngHuman CCL19/MIP-3 Beta ELISA Kit PicoKine®Human CCL19/MIP-3 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp-1-picokine-trade-elisa-kit-ek0458-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0458_2.pngHuman MMP-1 ELISA Kit PicoKine®Human MMP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp-2-picokine-trade-elisa-kit-ek0459-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0459.jpgHuman MMP-2 ELISA Kit PicoKine®Human MMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mmp-2-picokine-trade-elisa-kit-ek0460-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0460.jpgMouse MMP-2 ELISA Kit PicoKine®Mouse MMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp-3-picokine-trade-elisa-kit-ek0461-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0461_1.pngHuman MMP-3/Stromelysin-1 ELISA Kit PicoKine®Human MMP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mmp-3-picokine-trade-elisa-kit-ek0462-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0462.pngMouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®Mouse MMP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp-7-picokine-trade-elisa-kit-ek0463-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0463_2.pngHuman MMP-7/Matrilysin ELISA Kit PicoKine®Human MMP-7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp-8-picokine-trade-elisa-kit-ek0464-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0464.pngHuman MMP-8/Neutrophil collagenase ELISA Kit PicoKine®Human MMP-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp-9-picokine-trade-elisa-kit-ek0465-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0465.pngHuman MMP-9 ELISA Kit PicoKine®Human MMP-9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mmp-9-picokine-trade-elisa-kit-ek0466-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0466.pngMouse MMP-9 ELISA Kit PicoKine®Mouse MMP-9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp13-picokine-trade-elisa-kit-ek0468-boster.html2026-03-24T05:02:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0468_1.pngHuman MMP13/Collagenase 3 ELISA Kit PicoKine®Human MMP13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ngf-ngf-beta-picokine-trade-elisa-kit-ek0469-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0469_1.pngHuman Beta Nerve Growth Factor / NGF Beta / NGFB ELISA Kit PicoKine®Human NGF/NGF beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ngf-ngf-beta-picokine-trade-elisa-kit-ek0470-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0470_1.pngMouse NGF/NGF Beta ELISA Kit PicoKine®Mouse NGF/NGF beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-ngf-ngf-beta-picokine-trade-elisa-kit-ek0471-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0471.pngRat NGF/NGF Beta ELISA Kit PicoKine®Rat NGF/NGF beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-neurotrophin-3-picokine-trade-elisa-kit-ek0472-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0472.pngHuman Neurotrophin-3 ELISA Kit PicoKine®Human Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-neurotrophin-3-picokine-trade-elisa-kit-ek0473-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0473.pngMouse Neurotrophin-3 ELISA Kit PicoKine®Mouse Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-neurotrophin-3-picokine-trade-elisa-kit-ek0474-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0474.pngRat Neurotrophin-3 ELISA Kit PicoKine®Rat Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-nt-4-picokine-trade-elisa-kit-ek0475-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0475.pngHuman NT-4/NTF4/Neurotrophin-4 ELISA Kit PicoKine®Human NT-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-osm-oncostatin-m-picokine-trade-elisa-kit-ek0478-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0478.pngHuman OSM/Oncostatin M ELISA Kit PicoKine®Human OSM/Oncostatin M PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-opg-picokine-trade-elisa-kit-ek0480-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0480.jpgHuman OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®Human OPG PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-opg-picokine-trade-elisa-kit-ek0481-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0481.jpgMouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®Mouse OPG (TNFRSF11B) PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-opn-picokine-trade-elisa-kit-ek0482-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0482_1.pngHuman OPN / Osteopontin ELISA Kit PicoKine®Human OPN PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-opn-picokine-trade-elisa-kit-ek0483-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0483.pngMouse OPN / Osteopontin ELISA Kit PicoKine®Mouse OPN PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pdgf-ab-picokine-trade-elisa-kit-ek0484-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0484.pngHuman PDGF-AB ELISA Kit PicoKine®Human PDGF-AB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-pdgf-ab-picokine-trade-elisa-kit-ek0485-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0485.pngRat PDGF-AB ELISA Kit PicoKine®Rat PDGF-AB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-pdgf-ab-picokine-trade-elisa-kit-ek0486-boster.html2026-03-24T05:02:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0486.pngMouse PDGF-AB ELISA Kit PicoKine®Mouse PDGF-AB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-plgf-picokine-trade-elisa-kit-ek0490-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0490.pngHuman PLGF/PGF ELISA Kit PicoKine®Human PLGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-rantes-picokine-trade-elisa-kit-ek0494-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0494_1.jpgHuman Rantes ELISA Kit PicoKine®Human Rantes PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-rantes-picokine-trade-elisa-kit-ek0495-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0495.pngMouse Rantes ELISA Kit PicoKine®Mouse Rantes PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-rantes-picokine-trade-elisa-kit-ek0496-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0496.pngRat Rantes ELISA Kit PicoKine®Rat Rantes PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-se-selectin-picokine-trade-elisa-kit-ek0501-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0501_1.jpgHuman sE-Selectin / CD62E ELISA Kit PicoKine®Human sE-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-se-selectin-picokine-trade-elisa-kit-ek0502-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0502.pngMouse sE-Selectin ELISA Kit PicoKine®Mouse sE-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sl-selectin-picokine-trade-elisa-kit-ek0503-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0503.pngHuman sL-Selectin ELISA Kit PicoKine®Human sL-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-sl-selectin-picokine-trade-elisa-kit-ek0504-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0504.pngMouse sL-Selectin ELISA Kit PicoKine®Mouse L-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-p-selectin-picokine-trade-elisa-kit-ek0505-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0505_1.pngHuman P-Selectin / CD62P ELISA Kit PicoKine®Human P-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-p-selectin-picokine-trade-elisa-kit-ek0506-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0506.jpgMouse P-Selectin / CD62P ELISA Kit PicoKine®Mouse P-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-scf-picokine-trade-elisa-kit-ek0509-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0509.pngMouse SCF/KITLG/Kit ligand ELISA Kit PicoKine®Mouse SCF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tgf-alpha-picokine-trade-elisa-kit-ek0511-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0511_1.pngHuman TGF Alpha ELISA Kit PicoKine®Human TGF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tgf-beta-1-picokine-trade-elisa-kit-ek0513-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0513.jpgHuman TGF Beta 1 ELISA Kit PicoKine®Human TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-tgf-beta-1-picokine-trade-elisa-kit-ek0514-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0514.jpgRat TGF Beta 1 ELISA Kit PicoKine®Rat TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tgf-beta-1-picokine-trade-elisa-kit-ek0515-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0515.jpgMouse TGF Beta 1 ELISA Kit PicoKine®Mouse TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-thrombopoietin-tpo-picokine-trade-elisa-kit-ek0517-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0517.jpgMouse Thrombopoietin/TPO ELISA Kit PicoKine®Mouse TPO PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tie2-picokine-trade-elisa-kit-ek0519-boster.html2026-03-29T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0519.pngHuman TIE2/TEK ELISA Kit PicoKine®Human TIE2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-timp-1-picokine-trade-elisa-kit-ek0520-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0520.jpgHuman TIMP-1 ELISA Kit PicoKine®Human TIMP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-timp-2-picokine-trade-elisa-kit-ek0522-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0522.jpgHuman TIMP-2 ELISA Kit PicoKine®Human TIMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-timp-3-picokine-trade-elisa-kit-ek0523-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0523.pngHuman TIMP-3 ELISA Kit PicoKine®Human TIMP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-timp-4-picokine-trade-elisa-kit-ek0524-boster.html2026-03-24T05:02:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0524_1.jpgHuman TIMP-4 ELISA Kit PicoKine®Human TIMP-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnf-alpha-picokine-trade-elisa-kit-ek0525-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0525_2.pngHuman TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®Human TNF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-tnf-alpha-picokine-trade-elisa-kit-ek0526-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0526.pngRat TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®Rat TNF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnf-alpha-picokine-trade-elisa-kit-ek0527-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0527.jpgMouse TNF Alpha/Tumor necrosis factor ELISA Kit PicoKine®Mouse TNF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfsr-i-picokine-trade-elisa-kit-ek0528-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0528.jpgHuman TNFsR I ELISA Kit PicoKine®Human TNFsR I PicoKine ELISA Kit Standard Curve https://www.bosterbio.com/picokine-elisa-kits/human-stnfsr-ii-picokine-trade-elisa-kit-ek0530-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0530.jpgHuman sTNFsR II ELISA Kit PicoKine®Human sTNFsR II PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-stnfsr-ii-picokine-trade-elisa-kit-ek0531-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0531.pngMouse sTNFsR II ELISA Kit PicoKine®Mouse TNFR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-trail-picokine-trade-elisa-kit-ek0532-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0532.jpgHuman TRAIL / CD253 ELISA Kit PicoKine®Human TRAIL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-upa-plau-picokine-trade-elisa-kit-ek0535-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0535.pngHuman uPA / PLAU / URK ELISA Kit PicoKine®Human uPA/PLAU PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-upar-picokine-trade-elisa-kit-ek0536-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0536.jpgHuman uPAR / URKR / uPA Receptor ELISA Kit PicoKine®Human uPAR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-vcam-1-picokine-trade-elisa-kit-ek0537-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0537.jpgHuman VCAM-1 ELISA Kit PicoKine®Human VCAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-vcam-1-picokine-trade-elisa-kit-ek0538-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0538.pngMouse VCAM-1 ELISA Kit PicoKine®Mouse VCAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-vegf-picokine-trade-elisa-kit-ek0539-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0539_2.pngHuman VEGF ELISA Kit PicoKine®Human VEGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-vegf-picokine-trade-elisa-kit-ek0540-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0540.jpgRat VEGF ELISA Kit PicoKine®Rat VEGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-vegf-picokine-trade-elisa-kit-ek0541-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0541.jpgMouse VEGF ELISA Kit PicoKine®Mouse VEGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-svegfr1-sflt1-picokine-trade-elisa-kit-ek0543-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0543_1.pngHuman VEGFR1/sFLT1 ELISA Kit PicoKine®Human VEGFR1/sFLT1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-vegfr2-kdr-picokine-trade-elisa-kit-ek0544-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0544.jpgHuman VEGFR2 / KDR / VEGF Receptor 2 ELISA Kit PicoKine®Human VEGFR2/KDR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl21-6ckine-picokine-trade-elisa-kit-ek0555-boster.html2026-04-01T05:01:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0555.pngHuman CCL21 / 6Ckine / Exodus 2 ELISA Kit PicoKine®Human CCL21/6Ckine PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ace-picokine-trade-elisa-kit-ek0557-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0557.jpgHuman ACE/Cd143 ELISA Kit PicoKine®Human ACE PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ace-picokine-trade-elisa-kit-ek0558-boster.html2026-03-24T05:02:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0558.jpgMouse ACE/Cd143 ELISA Kit PicoKine®Mouse ACE PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-angiopoietin-1-picokine-trade-elisa-kit-ek0559-boster.html2026-03-29T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0559.pngHuman Angiopoietin-1 / ANG1 ELISA Kit PicoKine®Human Angiopoietin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-e-cadherin-picokine-trade-elisa-kit-ek0561-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0561.pngHuman E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®Human E-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-e-cadherin-picokine-trade-elisa-kit-ek0562-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0562.pngMouse E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®Mouse E-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cardiotrophin-1-picokine-trade-elisa-kit-ek0563-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0563.jpgHuman Cardiotrophin-1 / CTF1 ELISA Kit PicoKine®Human Cardiotrophin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl1-picokine-trade-elisa-kit-ek0565-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0565.pngHuman CCL1/I 309 ELISA Kit PicoKine®Human CCL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl1-picokine-trade-elisa-kit-ek0566-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0566.jpgMouse CCL1/I 309 ELISA Kit PicoKine®Mouse CCL1/TCA3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mcp-1-picokine-trade-elisa-kit-ek0568-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0568.pngMouse MCP-1 ELISA Kit PicoKine®Mouse MCP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd30-tnfrsf8-picokine-trade-elisa-kit-ek0570-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0570.pngMouse CD30/TNFRSF8 ELISA Kit PicoKine®Mouse CD30 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd30l-picokine-trade-elisa-kit-ek0572-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0572.pngMouse CD30L ELISA Kit PicoKine®Mouse CD30L PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-scd40l-picokine-trade-elisa-kit-ek0573-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0573.pngHuman CD40 Ligand/TNFSF5/CD40LG ELISA Kit PicoKine®Human sCD40L PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-eg-vegf-picokine-trade-elisa-kit-ek0575-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0575_1.pngHuman EG-VEGF/PK1/PROK1 ELISA Kit PicoKine®Human EG-VEGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl24-eotaxin-2-picokine-trade-elisa-kit-ek0576-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0576.pngMouse CCL24/Eotaxin-2 ELISA Kit PicoKine®Mouse CCL24/Eotaxin-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-gp130-il6st-picokine-trade-elisa-kit-ek0577-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0577.jpgMouse Gp130/IL6ST ELISA Kit PicoKine®Mouse Gp130/IL6ST PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-growth-hormone-picokine-trade-elisa-kit-ek0578-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0578.pngHuman Growth Hormone/GH1 ELISA Kit PicoKine®Human Growth Hormone PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-resistin-picokine-trade-elisa-kit-ek0581-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0581.jpgHuman Resistin ELISA Kit PicoKine®Human Resistin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-resistin-picokine-trade-elisa-kit-ek0582-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0582_1.pngMouse Resistin ELISA Kit PicoKine®Mouse Resistin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-timp-1-picokine-trade-elisa-kit-ek0583-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0583.pngRat TIMP-1 ELISA Kit PicoKine®Rat TIMP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnf-beta-picokine-trade-elisa-kit-ek0584-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0584.pngHuman TNF Beta/Lymphotoxin-alpha ELISA Kit PicoKine®Human TNF beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-vegf-c-picokine-trade-elisa-kit-ek0588-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0588.pngHuman VEGF-C ELISA Kit PicoKine®Human VEGF-C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-vegfr2-kdr-picokine-trade-elisa-kit-ek0590-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0590.pngMouse VEGFR2/KDR ELISA Kit PicoKine®Mouse VEGFR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-18-picokine-trade-elisa-kit-ek0592-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0592.pngRat IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®Rat IL-18 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-prolactin-picokine-trade-elisa-kit-ek0593-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0593.pngHuman Prolactin ELISA Kit PicoKine®Human Prolactin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-prolactin-picokine-trade-elisa-kit-ek0594-boster.html2026-03-24T05:02:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0594.pngMouse Prolactin ELISA Kit PicoKine®Mouse Prolactin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adiponectin-picokine-trade-elisa-kit-ek0595-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0595.jpgHuman Adiponectin ELISA Kit PicoKine®Human Adiponectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-adiponectin-picokine-trade-elisa-kit-ek0596-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0596.jpgMouse Adiponectin ELISA Kit PicoKine®Mouse Adiponectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-20-picokine-trade-elisa-kit-ek0599-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0599_1.pngHuman IL-20/Interleukin-20 ELISA Kit PicoKine®Human IL-20 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-mmp-2-picokine-trade-elisa-kit-ek0639-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0639.jpgRat MMP-2 ELISA Kit PicoKine®Rat MMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-survivin-picokine-trade-elisa-kit-ek0641-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0641.pngHuman Survivin ELISA Kit PicoKine®Human Survivin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tsp2-picokine-trade-elisa-kit-ek0642-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0642.jpgHuman TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®Human TSP2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd105-picokine-trade-elisa-kit-ek0644-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0644.pngHuman Endoglin/CD105/ENG ELISA Kit PicoKine®Human CD105 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-angiopoietin-2-picokine-trade-elisa-kit-ek0657-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0657.pngHuman Angiopoietin-2 / ANG2 ELISA Kit PicoKine®Human Angiopoietin-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-axl-picokine-trade-elisa-kit-ek0659-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0659.pngHuman AXL ELISA Kit PicoKine®Human AXL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-axl-picokine-trade-elisa-kit-ek0660-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0660.pngMouse AXL ELISA Kit PicoKine®Mouse AXL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf17-bcma-picokine-trade-elisa-kit-ek0661-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0661.pngHuman TNFRSF17/BCMA ELISA Kit PicoKine®Human TNFRSF17/BCMA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf17-bcma-picokine-trade-elisa-kit-ek0662-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0662.pngMouse TNFRSF17/BCMA ELISA Kit PicoKine®Mouse TNFRSF17/BCMA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-baff-picokine-trade-elisa-kit-ek0663-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0663.pngHuman BAFF / TNFSF13B ELISA Kit PicoKine®Human BAFF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-baff-picokine-trade-elisa-kit-ek0664-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0664.jpgMouse BAFF ELISA Kit PicoKine®Mouse BAFF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-p-cadherin-picokine-trade-elisa-kit-ek0667-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0667.pngHuman P-Cadherin-3 CDH3 ELISA Kit PicoKine®Human P-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-p-cadherin-picokine-trade-elisa-kit-ek0668-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0668.pngMouse P-Cadherin-3 Cdh3 ELISA Kit PicoKine®Mouse P-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-b-picokine-trade-elisa-kit-ek0670-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0670.pngHuman Cathepsin B ELISA Kit PicoKine®Human Cathepsin B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cathepsin-b-picokine-trade-elisa-kit-ek0671-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0671_2.pngMouse Cathepsin B ELISA Kit PicoKine®Mouse Cathepsin B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-d-picokine-trade-elisa-kit-ek0672-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0672.pngHuman Cathepsin D ELISA Kit PicoKine®Human Cathepsin D PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cathepsin-d-picokine-trade-elisa-kit-ek0673-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0673.jpgMouse Cathepsin D ELISA Kit PicoKine®Mouse Cathepsin D PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cystatin-c-picokine-trade-elisa-kit-ek0678-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0678_1.jpgHuman Cystatin C/CST3 ELISA Kit PicoKine®Human Cystatin C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cystatin-c-picokine-trade-elisa-kit-ek0679-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0679.jpgMouse Cystatin C/CST3 ELISA Kit PicoKine®Mouse Cystatin C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ca9-picokine-trade-elisa-kit-ek0682-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0682.pngHuman CA9/Carbonic anhydrase 9 ELISA Kit PicoKine®Human CA9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl7-mcp-3-picokine-trade-elisa-kit-ek0683-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0683.pngMouse CCL7/MCP-3 ELISA Kit PicoKine®Mouse CCL7/MCP3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl17-tarc-picokine-trade-elisa-kit-ek0684-boster.html2026-03-24T05:02:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0684_1.pngHuman CCL17/TARC ELISA Kit PicoKine®Human CCL17/TARC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl17-tarc-picokine-trade-elisa-kit-ek0685-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0685.jpgMouse CCL17/TARC ELISA Kit PicoKine®Mouse CCL17/TARC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl18-parc-picokine-trade-elisa-kit-ek0686-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0686.jpgHuman CCL18/PARC ELISA Kit PicoKine®Human CCL18/PARC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl27-ctack-picokine-trade-elisa-kit-ek0688-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0688.pngHuman CCL27/CTACK ELISA Kit PicoKine®Human CCL27/CTACK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl28-picokine-trade-elisa-kit-ek0691-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0691.jpgMouse CCL28 ELISA Kit PicoKine®Mouse CCL28 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd14-picokine-trade-elisa-kit-ek0695-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0695.jpgMouse CD14 ELISA Kit PicoKine®Mouse CD14 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd26-dpp4-picokine-trade-elisa-kit-ek0696-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0696.jpgHuman CD26/DPP4 ELISA Kit PicoKine®Human CD26/DPP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd26-dpp4-picokine-trade-elisa-kit-ek0697-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0697.pngMouse CD26/DPP4 ELISA Kit PicoKine®Mouse CD26/DPP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf7-cd27-picokine-trade-elisa-kit-ek0698-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0698.pngHuman TNFRSF7/CD27 ELISA Kit PicoKine®Human TNFRSF7/CD27 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf7-cd27-picokine-trade-elisa-kit-ek0699-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0699_2.pngMouse TNFRSF7/CD27 ELISA Kit PicoKine®Mouse TNFRSF7/CD27 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd36-sr-b3-picokine-trade-elisa-kit-ek0700-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0700.pngHuman CD36/SR-B3/GP4 ELISA Kit PicoKine®Human CD36/SR-B3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd36-sr-b3-picokine-trade-elisa-kit-ek0701-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0701.pngMouse CD36/SR-B3/GP4 ELISA Kit PicoKine®Mouse CD36/SR-B3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd40-tnfrsf5-picokine-trade-elisa-kit-ek0702-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0702.pngHuman CD40/TNFRSF5 ELISA Kit PicoKine®Human CD40/TNFRSF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd40-tnfrsf5-picokine-trade-elisa-kit-ek0703-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0703.pngMouse CD40/TNFRSF5 ELISA Kit PicoKine®Mouse CD40/TNFRSF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd56-ncam-1-picokine-trade-elisa-kit-ek0706-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0706.pngHuman CD56/NCAM-1 ELISA Kit PicoKine®Human CD56/NCAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-b7-1-cd80-picokine-trade-elisa-kit-ek0707-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0707.pngHuman B7-1/CD80 ELISA Kit PicoKine®Human B7-1/CD80 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-b7-1-cd80-picokine-trade-elisa-kit-ek0708-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0708.jpgMouse B7-1/CD80 ELISA Kit PicoKine®Mouse B7-1/CD80 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-b7-1-cd80-picokine-trade-elisa-kit-ek0709-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0709.pngRat B7-1/CD80 ELISA Kit PicoKine®Rat CD80/B7-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cd86-b7-2-picokine-trade-elisa-kit-ek0712-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0712.pngRat CD86/B7-2 ELISA Kit PicoKine®Rat CD86/B7-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il7r-cd127-picokine-trade-elisa-kit-ek0713-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0713.jpgHuman IL7R/CD127 ELISA Kit PicoKine®Human IL7R/CD127 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd163-picokine-trade-elisa-kit-ek0715-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0715.jpgHuman CD163 / M130 ELISA Kit PicoKine®Human CD163 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ctla4-picokine-trade-elisa-kit-ek0717-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0717.pngMouse CTLA4 ELISA Kit PicoKine®Mouse CTLA4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl1-picokine-trade-elisa-kit-ek0722-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0722.jpgHuman CXCL1/Gro Alpha ELISA Kit PicoKine®Human CXCL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl1-picokine-trade-elisa-kit-ek0723-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0723.pngMouse CXCL1/Gro Alpha ELISA Kit PicoKine®Mouse CXCL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cxcl1-picokine-trade-elisa-kit-ek0724-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0724.pngRat CXCL1/Gro Alpha ELISA Kit PicoKine®Rat CXCL1/Gro Alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl4-pf4-picokine-trade-elisa-kit-ek0727-boster.html2026-03-24T05:02:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0727.pngMouse CXCL4/PF4 ELISA Kit PicoKine®Mouse CXCL4/PF4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl5-ena-78-picokine-trade-elisa-kit-ek0728-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0728.pngHuman CXCL5/LIX/ENA-78 ELISA Kit PicoKine®Human CXCL5/ENA-78 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl7-picokine-trade-elisa-kit-ek0729-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0729.pngHuman CXCL7/PPBP/NAP-2 ELISA Kit PicoKine®Human CXCL7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl9-picokine-trade-elisa-kit-ek0732-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0732_1.pngHuman CXCL9/Mig ELISA Kit PicoKine®Human CXCL9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl10-ip-10-picokine-trade-elisa-kit-ek0735-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0735.pngHuman CXCL10/IP-10 ELISA Kit PicoKine®Human CXCL10/IP-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl10-ip-10-picokine-trade-elisa-kit-ek0736-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0736.pngMouse CXCL10/IP-10 ELISA Kit PicoKine®Mouse CXCL10/IP-10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl11-i-tac-picokine-trade-elisa-kit-ek0737-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0737.pngHuman CXCL11/I-TAC ELISA Kit PicoKine®Human CXCL11/I-TAC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl13-blc-picokine-trade-elisa-kit-ek0739-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0739.pngHuman CXCL13 / BLC / BCA1 ELISA Kit PicoKine®Human CXCL13/BLC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl13-blc-picokine-trade-elisa-kit-ek0740-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0740.pngMouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine®Mouse CXCL13/BLC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl16-picokine-trade-elisa-kit-ek0741-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0741.jpgHuman CXCL16 ELISA Kit PicoKine®Human CXCL16 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl16-picokine-trade-elisa-kit-ek0742-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0742.pngMouse CXCL16 ELISA Kit PicoKine®Mouse CXCL16 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-c-met-hgfr-picokine-trade-elisa-kit-ek0744-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0744.pngHuman C-MET/HGFR ELISA Kit PicoKine®Human C-MET/HGFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-hgfr-picokine-trade-elisa-kit-ek0745-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0745.jpgMouse HGFR/c-MET ELISA Kit PicoKine&reg;Mouse C-MET/HGFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-decorin-picokine-trade-elisa-kit-ek0749-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0749.pngHuman Decorin / DCN ELISA Kit PicoKine®Human Decorin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-decorin-picokine-trade-elisa-kit-ek0750-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0750.jpgMouse Decorin ELISA Kit PicoKine®Mouse Decorin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-emmprin-picokine-trade-elisa-kit-ek0751-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0751_3.pngHuman EMMPRIN/CD147/BSG ELISA Kit PicoKine®Human Emmprin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-esm1-endocan-picokine-trade-elisa-kit-ek0752-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0752.pngHuman ESM1/Endocan ELISA Kit PicoKine®Human ESM1/Endocan PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-esm1-endocan-picokine-trade-elisa-kit-ek0753-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0753.jpgMouse ESM1/Endocan ELISA Kit PicoKine®Mouse ESM1/Endocan PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-erbb-2-picokine-trade-elisa-kit-ek0756-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0756.pngHuman ErbB-2 ELISA Kit PicoKine®Human ErbB-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fetuin-a-picokine-trade-elisa-kit-ek0757-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0757.jpgHuman Fetuin A/AHSG ELISA Kit PicoKine®Human Fetuin A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-galectin-1-picokine-trade-elisa-kit-ek0763-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0763_1.pngMouse Galectin-1 ELISA Kit PicoKine®Mouse Galectin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-galectin-3-lgals3-picokine-trade-elisa-kit-ek0764-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0764.pngHuman Galectin-3/LGALS3 ELISA Kit PicoKine®Human Galectin-3/LGALS3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-galectin-3-lgals3-picokine-trade-elisa-kit-ek0765-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0765.pngMouse Galectin-3/LGALS3 ELISA Kit PicoKine®Mouse Galectin-3/LGALS3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gdf-15-picokine-trade-elisa-kit-ek0767-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0767.pngHuman GDF-15 ELISA Kit PicoKine®Human GDF-15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf18-gitr-picokine-trade-elisa-kit-ek0768-boster.html2026-03-24T05:02:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0768.pngHuman TNFRSF18/GITR ELISA Kit PicoKine®Human TNFRSF18/GITR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf18-gitr-picokine-trade-elisa-kit-ek0769-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0769.pngMouse TNFRSF18/GITR ELISA Kit PicoKine®Mouse TNFRSF18/GITR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hbegf-picokine-trade-elisa-kit-ek0770-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0770.jpgHuman HBEGF ELISA Kit PicoKine®Human HBEGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-6r-alpha-picokine-trade-elisa-kit-ek0777-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0777.jpgHuman IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®Human IL-6R alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-7-picokine-trade-elisa-kit-ek0779-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0779.jpgHuman IL-7/Interleukin-7 ELISA Kit PicoKine®Human IL-7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-7-picokine-trade-elisa-kit-ek0780-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0780.pngMouse IL-7/Interleukin-7 ELISA Kit PicoKine®Mouse IL-7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-1ra-picokine-trade-elisa-kit-ek0782-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0782.jpgHuman IL-1RA/IL1RN ELISA Kit PicoKine®Human IL-1RA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-1ra-il1rn-picokine-trade-elisa-kit-ek0783-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0783.pngMouse IL-1RA/IL1RN ELISA Kit PicoKine®Mouse IL-1RA/IL1RN PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-17f-picokine-trade-elisa-kit-ek0796-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0796.pngMouse IL-17F/Interleukin-17F ELISA Kit PicoKine®Mouse IL-17F PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-21-picokine-trade-elisa-kit-ek0797-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0797.pngMouse IL-21/Interleukin-21 ELISA Kit PicoKine®Mouse IL-21 PicoKine ELISA Kit standard curve A https://www.bosterbio.com/picokine-elisa-kits/human-il-27-picokine-trade-elisa-kit-ek0799-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0799.pngHuman IL-27 ELISA Kit PicoKine®Human IL-27 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-27-picokine-trade-elisa-kit-ek0800-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0800.pngMouse IL-27/IL-30 p28 ELISA Kit PicoKine®Mouse IL-27 p28 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-xcl1-lymphotactin-picokine-trade-elisa-kit-ek0802-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0802.pngHuman XCL1/Lymphotactin ELISA Kit PicoKine®Human XCL1/Lymphotactin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-csf1r-m-csfr-picokine-trade-elisa-kit-ek0807-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0807.pngHuman CSF1R/M-CSFR ELISA Kit PicoKine®Human CSF1R/M-CSFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-csf1r-m-csfr-picokine-trade-elisa-kit-ek0808-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0808_1.pngMouse CSF1R/M-CSFR ELISA Kit PicoKine®Mouse CSF1R/M-CSFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl13-mcp4-picokine-trade-elisa-kit-ek0809-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0809.jpgHuman CCL13/MCP4 ELISA Kit PicoKine®Human CCL13/MCP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mer-picokine-trade-elisa-kit-ek0810-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0810.pngHuman Mer ELISA Kit PicoKine®Human Mer PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mer-picokine-trade-elisa-kit-ek0811-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0811.jpgMouse Mer ELISA Kit PicoKine®Mouse MER/MERTK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mica-picokine-trade-elisa-kit-ek0812-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0812.pngHuman MICA ELISA Kit PicoKine®Human MICA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mif-picokine-trade-elisa-kit-ek0813-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0813.pngHuman MIF ELISA Kit PicoKine®Human MIF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-msp-mst1-picokine-trade-elisa-kit-ek0814-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0814.pngHuman MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine®Human MSP/MST1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-kallikrein-3-picokine-trade-elisa-kit-ek0816-boster.html2026-03-24T05:02:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0816.jpgHuman Prostate-specific antigen ELISA kit (PSA/KLK3) PicoKine®Human Kallikrein 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-klk5-picokine-trade-elisa-kit-ek0817-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0817.jpgHuman KLK5/Kallikrein-5 ELISA Kit PicoKine®Human KLK5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-kallikrein-6-picokine-trade-elisa-kit-ek0818-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0818.jpgHuman Kallikrein-6 / KLK6 ELISA Kit PicoKine®Human Kallikrein-6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fgf7-kgf-picokine-trade-elisa-kit-ek0820-boster.html2026-04-01T05:01:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0820.jpgHuman FGF7/KGF ELISA Kit PicoKine®Human FGF7/KGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lox-1-olr1-picokine-trade-elisa-kit-ek0824-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0824.pngHuman LOX-1/OLR1 ELISA Kit PicoKine®Human LOX-1/OLR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-lox-1-olr1-picokine-trade-elisa-kit-ek0825-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0825.pngMouse LOX-1/OLR1 ELISA Kit PicoKine®Mouse LOX-1/OLR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-rage-picokine-trade-elisa-kit-ek0827-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0827.jpgHuman Rage ELISA Kit PicoKine®Human Rage PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-rage-picokine-trade-elisa-kit-ek0828-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0828.jpgMouse RAGE ELISA Kit PicoKine®Mouse RAGE PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-rank-picokine-trade-elisa-kit-ek0829-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0829.pngHuman RANK ELISA Kit PicoKine®Human RANK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-rank-picokine-trade-elisa-kit-ek0830-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0830.pngMouse RANK / CD265 ELISA Kit PicoKine®Mouse RANK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-rbp4-picokine-trade-elisa-kit-ek0831-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0831.jpgHuman RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®Human RBP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-rbp4-picokine-trade-elisa-kit-ek0832-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0832.jpgMouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®Mouse RBP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-nov-ccn3-picokine-trade-elisa-kit-ek0833-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0833.pngHuman NOV/CCN3 ELISA Kit PicoKine®Human NOV/CCN3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-kit-scfr-picokine-trade-elisa-kit-ek0835-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0835.pngHuman KIT / SCFR / CD117 / c Kit ELISA Kit PicoKine®Human KIT/SCFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-kallistatin-serpina4-picokine-trade-elisa-kit-ek0836-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0836.jpgHuman Kallistatin/Serpina4 ELISA Kit PicoKine®Human Kallistatin/Serpina4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-diablo-smac-picokine-trade-elisa-kit-ek0838-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0838.pngHuman Diablo/SMAC ELISA Kit PicoKine®Human Diablo/SMAC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tfpi-picokine-trade-elisa-kit-ek0840-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0840.pngHuman TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®Human TFPI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfsf11-rankl-picokine-trade-elisa-kit-ek0842-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0842.jpgHuman TNFSF11/RANKL ELISA Kit PicoKine®Human TNFSF11/RANKL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfsf11-rankl-picokine-trade-elisa-kit-ek0843-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0843_2.pngMouse TNFSF11/RANKL ELISA Kit PicoKine®Mouse TNFSF11/RANKL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-trka-picokine-trade-elisa-kit-ek0846-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0846.pngHuman TrkA ELISA Kit PicoKine®Human TrkA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-trka-picokine-trade-elisa-kit-ek0847-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0847.jpgRat TrkA ELISA Kit PicoKine®Rat TrkA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-trkb-picokine-trade-elisa-kit-ek0849-boster.html2026-03-24T05:02:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0849.jpgMouse TrkB ELISA Kit PicoKine®Mouse TrkB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mpo-picokine-trade-elisa-kit-ek0850-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0850.jpgHuman MPO/Myeloperoxidase ELISA Kit PicoKine®Human MPO PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd10-neprilysin-picokine-trade-elisa-kit-ek0851-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0851.jpgHuman CD10/Neprilysin ELISA Kit PicoKine®Human CD10/Neprilysin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd10-neprilysin-picokine-trade-elisa-kit-ek0852-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0852.jpgMouse CD10/Neprilysin ELISA Kit PicoKine®Mouse Neprilysin/CD10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lipocalin-2-ngal-picokine-trade-elisa-kit-ek0853-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0853_1.pngHuman Lipocalin-2/NGAL ELISA Kit PicoKine®Human Lipocalin-2/NGAL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-lipocalin-2-ngal-picokine-trade-elisa-kit-ek0854-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0854.jpgMouse Lipocalin-2/NGAL ELISA Kit PicoKine®Mouse Lipocalin-2/NGAL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-lipocalin-2-ngal-picokine-trade-elisa-kit-ek0855-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0855.jpgRat Lipocalin-2/NGAL ELISA Kit PicoKine®Rat Lipocalin-2/NGAL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-nidogen-1-entactin-nid-1-picokine-trade-elisa-kit-ek0856-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0856.pngHuman Nidogen-1 / Entactin / NID-1 / NID1 ELISA Kit PicoKine®Human Nidogen-1/Entactin/NID-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfsf4-ox40l-picokine-trade-elisa-kit-ek0857-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0857_1.pngHuman TNFSF4/OX40L ELISA Kit PicoKine®Human TNFSF4/OX40L PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfsf4-ox40l-picokine-trade-elisa-kit-ek0858-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0858.pngMouse TNFSF4/OX40L ELISA Kit PicoKine®Mouse TNFSF4/OX40L PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pai-1-picokine-trade-elisa-kit-ek0859-boster.html2026-04-03T05:00:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0859.pngHuman PAI-1 ELISA Kit PicoKine®Human PAI-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ptx3-pentraxin-3-picokine-trade-elisa-kit-ek0861-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0861.jpgHuman PTX3/Pentraxin 3 ELISA Kit PicoKine®Human PTX3/Pentraxin 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ptx3-pentraxin-3-picokine-trade-elisa-kit-ek0862-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0862.pngMouse PTX3/Pentraxin 3 ELISA Kit PicoKine®Mouse PTX3/Pentraxin 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-18-picokine-trade-elisa-kit-ek0864-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0864.pngHuman IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®Human IL-18 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-23-picokine-trade-elisa-kit-ek0866-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0866.pngMouse IL-23 ELISA Kit PicoKine®Mouse IL-23 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dkk-1-picokine-trade-elisa-kit-ek0867-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0867.pngHuman DKK-1 / Dickkopf 1 ELISA Kit PicoKine®Human DKK-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-flrg-fstl3-picokine-trade-elisa-kit-ek0869-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0869.pngHuman FLRG/FSTL3 ELISA Kit PicoKine®Human FLRG/FSTL3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pecam-1-cd31-picokine-trade-elisa-kit-ek0873-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0873.jpgHuman PECAM-1/CD31 ELISA Kit PicoKine®Human PECAM-1/CD31 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-pecam-1-cd31-picokine-trade-elisa-kit-ek0874-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0874.pngMouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine®Mouse PECAM-1/CD31 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-kim1-picokine-trade-elisa-kit-ek0880-boster.html2026-04-03T05:00:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0880.pngMouse KIM1 / TIM1 ELISA Kit PicoKine®Mouse KIM1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hsp27-picokine-trade-elisa-kit-ek0881-boster.html2026-03-24T05:02:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0881.pngHuman HSP27/HSPB1/Heat Shock Protein 27 ELISA Kit PicoKine®Human HSP27 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-kim1-picokine-trade-elisa-kit-ek0882-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0882_1.pngRat KIM1 ELISA Kit PicoKine®Rat KIM1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-kim1-picokine-trade-elisa-kit-ek0883-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0883.pngHuman KIM1 / TIM-1 ELISA Kit PicoKine®Human KIM1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-bmp-7-picokine-trade-elisa-kit-ek0884-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0884.pngHuman BMP-7 ELISA Kit PicoKine®Human BMP-7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-endostatin-picokine-trade-elisa-kit-ek0886-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0886.pngHuman Endostatin ELISA Kit PicoKine®Human Endostatin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-p53-picokine-trade-elisa-kit-ek0895-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0895.pngHuman P53 ELISA Kit PicoKine®Human P53 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-plat-tpa-picokine-trade-elisa-kit-ek0897-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0897.pngHuman PLAT/TPA ELISA Kit PicoKine®Human PLAT/TPA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-thbs1-tsp1-picokine-trade-elisa-kit-ek0899-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0899.jpgHuman THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®Human THBS1/TSP1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-13-picokine-trade-elisa-kit-ek0900-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0900.pngRat IL-13/Interleukin-13 ELISA Kit PicoKine®Rat IL-13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-mcp-1-picokine-trade-elisa-kit-ek0902-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0902.pngRat MCP-1 ELISA Kit PicoKine®Rat MCP-1/CCL2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-klk1-picokine-trade-elisa-kit-ek0903-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0903_1.pngHuman KLK1/Kallikrein 1 ELISA Kit PicoKine®Human KLK1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cea-picokine-trade-elisa-kit-ek0904-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0904.jpgHuman CEA / Carcino Embryonic Antigen ELISA Kit PicoKine®Human CEA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-angiostatin-k1-3-picokine-trade-elisa-kit-ek0905-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0905.pngHuman Angiostatin Kringle 1-3/PLG ELISA Kit PicoKine®Human Angiostatin K1-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tlr2-picokine-trade-elisa-kit-ek0906-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0906.pngHuman TLR2/Toll-like receptor 2 ELISA Kit PicoKine®Human TLR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tlr3-picokine-trade-elisa-kit-ek0907-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0907.pngHuman TLR3/Toll-like receptor 3 ELISA Kit PicoKine®Human TLR3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd23-fcer2-picokine-trade-elisa-kit-ek0912-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0912.pngHuman CD23/FCER2 ELISA Kit PicoKine®Human CD23/FCER2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-comp-picokine-trade-elisa-kit-ek0913-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0913_1.pngHuman COMP/Thrombospondin 5 ELISA Kit PicoKine®Human COMP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-clusterin-picokine-trade-elisa-kit-ek0914-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0914.jpgHuman Clusterin ELISA Kit PicoKine®Human Clusterin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd25-il-2sr-alpha-picokine-trade-elisa-kit-ek0915-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0915.jpgMouse CD25/IL-2sR Alpha ELISA Kit PicoKine®Mouse CD25/IL-2sR alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-furin-picokine-trade-elisa-kit-ek0916-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0916.jpgHuman Furin ELISA Kit PicoKine®Human Furin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-thrombomodulin-picokine-trade-elisa-kit-ek0917-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0917.pngHuman Thrombomodulin ELISA Kit PicoKine®Human Thrombomodulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl5-ena-78-picokine-trade-elisa-kit-ek0919-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0919.pngMouse CXCL5 / LIX / ENA-78 / GCP 2 ELISA Kit PicoKine®Mouse CXCL5/ENA-78 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfsf12-picokine-trade-elisa-kit-ek0920-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0920.pngHuman TNFSF12/Tweak ELISA Kit PicoKine®Human TNFSF12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfsf13-april-picokine-trade-elisa-kit-ek0921-boster.html2026-03-24T05:02:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0921.pngHuman TNFSF13/APRIL ELISA Kit PicoKine®Human TNFSF13/APRIL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-scgb1a1-uteroglobin-picokine-trade-elisa-kit-ek0922-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0922.pngHuman SCGB1A1/uteroglobin ELISA Kit PicoKine®Human SCGB1A1/uteroglobin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-clusterin-picokine-trade-elisa-kit-ek0923-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0923.jpgMouse Clusterin / Apolipoprotein J ELISA Kit PicoKine®Mouse Clusterin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-dkk-1-picokine-trade-elisa-kit-ek0925-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0925.jpgMouse DKK-1 ELISA Kit PicoKine®Mouse DKK1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd244-2b4-picokine-trade-elisa-kit-ek0926-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0926.pngHuman CD244/2B4 ELISA Kit PicoKine®Human CD244/2B4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tissue-factor-f3-picokine-trade-elisa-kit-ek0928-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0928.pngHuman Tissue Factor/F3 ELISA Kit PicoKine®Human Tissue factor/F3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-33-picokine-trade-elisa-kit-ek0929-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0929.pngHuman IL-33 ELISA Kit PicoKine®Human IL-33 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-33-picokine-trade-elisa-kit-ek0930-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0930.pngMouse IL-33 ELISA Kit PicoKine®Mouse IL-33 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-12-p40-picokine-trade-elisa-kit-ek0932-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0932.pngMouse IL-12(p40) ELISA Kit PicoKine®Mouse IL-12(p40) PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-22-picokine-trade-elisa-kit-ek0933-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0933_2.pngHuman IL-22/Interleukin-22 ELISA Kit PicoKine®Human IL-22 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adam12-picokine-trade-elisa-kit-ek0934-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0934.pngHuman ADAM12 / MCMP ELISA Kit PicoKine®Human ADAM12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-gdnf-picokine-trade-elisa-kit-ek0935-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0935_1.pngMouse GDNF ELISA Kit PicoKine®Mouse GDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-se-selectin-picokine-trade-elisa-kit-ek0936-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0936.pngRat sE-Selectin ELISA Kit PicoKine®Rat sE-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-sl-selectin-picokine-trade-elisa-kit-ek0937-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0937.jpgRat sL-Selectin ELISA Kit PicoKine®Rat sL-Selectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-angiopoietin-2-picokine-trade-elisa-kit-ek0938-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0938.pngMouse Angiopoietin-2 ELISA Kit PicoKine®Mouse Angiopoietin-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-ccl4-mip-1-beta-picokine-trade-elisa-kit-ek0939-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0939.pngRat CCL4/MIP-1 Beta ELISA Kit PicoKine®Rat CCL4/MIP-1 beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-icam-3-picokine-trade-elisa-kit-ek0940-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0940.pngHuman ICAM-3 / CD50 ELISA Kit PicoKine®Human ICAM-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mia-picokine-trade-elisa-kit-ek0941-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0941_1.pngHuman MIA ELISA Kit PicoKine®Human MIA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-myd88-picokine-trade-elisa-kit-ek0942-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0942.pngHuman MyD88 ELISA Kit PicoKine®Human MyD88 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mpo-picokine-trade-elisa-kit-ek0943-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0943.pngMouse MPO/Myeloperoxidase ELISA Kit PicoKine®Mouse MPO PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-endothelin-1-picokine-trade-elisa-kit-ek0945-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0945.jpgHuman Endothelin ELISA Kit PicoKine®Human Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-kallikrein-14-picokine-trade-elisa-kit-ek0949-boster.html2026-03-24T05:02:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0949.pngHuman Kallikrein 14 ELISA Kit PicoKine®Human Kallikrein 14 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mmp-12-picokine-trade-elisa-kit-ek0950-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0950_1.pngHuman MMP-12 ELISA Kit PicoKine®Human MMP-12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-endothelin-1-picokine-trade-elisa-kit-ek0952-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0952.jpgRat Endothelin 1/EDN1 ELISA Kit PicoKine®Rat Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-endothelin-1-picokine-trade-elisa-kit-ek0953-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0953.jpgMouse Endothelin 1/EDN1 ELISA Kit PicoKine®Mouse Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-egf-picokine-trade-elisa-kit-ek0954-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0954_1.jpgRat EGF ELISA Kit PicoKine®Rat EGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-epcr-picokine-trade-elisa-kit-ek0957-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0957.jpgHuman EPCR ELISA Kit PicoKine®Human EPCR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tslp-picokine-trade-elisa-kit-ek0958-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0958.pngHuman TSLP ELISA Kit PicoKine®Human TSLP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pd-1-picokine-trade-elisa-kit-ek0959-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0959.pngHuman PD-1 ELISA Kit PicoKine®Human PD-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-angptl4-picokine-trade-elisa-kit-ek0960-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0960.jpgHuman ANGPTL4 / Angiopoietin Like 4 / ARP4 ELISA Kit PicoKine®Human ANGPTL4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-s-picokine-trade-elisa-kit-ek0961-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0961.pngHuman Cathepsin S / CTSS ELISA Kit PicoKine®Human Cathepsin S PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-igfbp6-picokine-trade-elisa-kit-ek0962-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0962.pngHuman IGFBP6 ELISA Kit PicoKine®Human IGFBP6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-micb-picokine-trade-elisa-kit-ek0963-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0963.pngHuman MICB ELISA Kit PicoKine®Human MICB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-29-picokine-trade-elisa-kit-ek0964-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0964.pngHuman IL-29 ELISA Kit PicoKine®Human IL-29 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fstl1-picokine-trade-elisa-kit-ek0965-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0965.jpgHuman FSTL1/Tsc 36 ELISA Kit PicoKine®Human FSTL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-complement-c5a-picokine-trade-elisa-kit-ek0966-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0966_1.pngHuman complement C5a ELISA Kit PicoKine®Human complement C5a PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lyve-1-picokine-trade-elisa-kit-ek0967-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0967.pngHuman LYVE-1 ELISA Kit PicoKine®Human LYVE-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-28a-picokine-trade-elisa-kit-ek0969-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0969.jpgHuman IL-28A ELISA Kit PicoKine®Human IL-28A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-rage-picokine-trade-elisa-kit-ek0971-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0971.jpgRat RAGE ELISA Kit PicoKine®Rat RAGE PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-progranulin-picokine-trade-elisa-kit-ek0973-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0973.jpgHuman Progranulin ELISA Kit PicoKine®Human Progranulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-chitinase-3-like-1-ykl-40-picokine-trade-elisa-kit-ek0974-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0974.pngHuman Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®Human Chitinase 3-like 1/YKL-40 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-chitinase-3-like-1-ykl-40-picokine-trade-elisa-kit-ek0975-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0975.jpgMouse Chitinase 3-like 1/YKL-40 ELISA Kit PicoKine®Mouse Chitinase 3-like 1/YKL-40 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tek-picokine-trade-elisa-kit-ek0976-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0976.jpgMouse TIE2/TEK ELISA Kit PicoKine®Mouse TEK/TIE2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-crp-picokine-trade-elisa-kit-ek0977-boster.html2026-03-24T05:02:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0977_2.pngMouse CRP / C Reactive Protein / PTX1 ELISA Kit PicoKine®Mouse CRP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-crp-picokine-trade-elisa-kit-ek0978-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0978_1.pngRat CRP/C Reactive Protein ELISA Kit PicoKine®Rat CRP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-31-picokine-trade-elisa-kit-ek0979-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0979_1.pngHuman IL-31/Interleukin-31 ELISA Kit PicoKine®Human IL-31 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-betacellulin-btc-picokine-trade-elisa-kit-ek0980-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0980.pngHuman Betacellulin/BTC ELISA Kit PicoKine®Human Betacellulin/BTC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tgf-beta-2-picokine-trade-elisa-kit-ek0981-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981.pngHuman TGF-Beta 2 ELISA Kit PicoKine®Human TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-tgf-beta-2-picokine-trade-elisa-kit-ek0982-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0982.pngRat TGF-Beta 2 ELISA Kit PicoKine®Rat TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tgf-beta-2-picokine-trade-elisa-kit-ek0983-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0983_1.pngMouse TGF-Beta 2 ELISA Kit PicoKine®Mouse TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-17rc-picokine-trade-elisa-kit-ek0984-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0984.jpgHuman IL-17RC ELISA Kit PicoKine®Human IL-17RC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-periostin-osf2-picokine-trade-elisa-kit-ek0985-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0985_3.pngHuman Periostin/OSF2 ELISA Kit PicoKine®Human Periostin/OSF2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf13b-taci-picokine-trade-elisa-kit-ek0986-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0986.jpgHuman TNFRSF13B/TACI ELISA Kit PicoKine®Human TNFRSF13B/TACI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-complement-c5a-picokine-trade-elisa-kit-ek0987-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0987.pngMouse Complement C5a ELISA Kit PicoKine®Mouse Complement C5a PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf9-4-1bb-picokine-trade-elisa-kit-ek0988-boster.html2026-04-01T05:01:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0988.pngHuman TNFRSF9/4-1BB ELISA Kit PicoKine®Human TNFRSF9/4-1BB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfsf14-light-picokine-trade-elisa-kit-ek0990-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0990.pngHuman TNFSF14/LIGHT ELISA Kit PicoKine®Human TNFSF14/LIGHT PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-igfbp7-picokine-trade-elisa-kit-ek0991-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0991.jpgHuman IGFBP7/Igfbp Rp1 ELISA Kit PicoKine®Human IGFBP7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd200-picokine-trade-elisa-kit-ek0993-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0993.pngHuman CD200 ELISA Kit PicoKine®Human CD200 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fgf21-picokine-trade-elisa-kit-ek0994-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0994_1.pngHuman FGF21 ELISA Kit PicoKine®Human FGF21 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-alcam-picokine-trade-elisa-kit-ek0995-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0995.jpgHuman ALCAM/CD166 ELISA Kit PicoKine®Human ALCAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mmp-12-picokine-trade-elisa-kit-ek0996-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0996.pngMouse MMP-12 ELISA Kit PicoKine®Mouse MMP-12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf4-ox40-picokine-trade-elisa-kit-ek0998-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0998.pngMouse TNFRSF4/OX40 ELISA Kit PicoKine®Mouse TNFRSF4/OX40 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-icam-2-picokine-trade-elisa-kit-ek0999-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0999.jpgHuman ICAM-2 ELISA Kit PicoKine®Human ICAM-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-31-picokine-trade-elisa-kit-ek1100-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1100_1.pngMouse IL-31/Interleukin-31 ELISA Kit PicoKine®Mouse IL-31 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-seprase-fap-picokine-trade-elisa-kit-ek1101-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1101_1.pngHuman Seprase/FAP ELISA Kit PicoKine®Human Seprase/FAP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tgf-beta-3-picokine-trade-elisa-kit-ek1103-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1103_1.pngHuman TGF-Beta 3 ELISA Kit PicoKine®Human TGF-Beta3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tgf-beta-3-picokine-trade-elisa-kit-ek1104-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1104.pngMouse TGF-Beta 3 ELISA Kit PicoKine®Mouse TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-tgf-beta-3-picokine-trade-elisa-kit-ek1105-boster.html2026-03-24T05:03:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1105.pngRat TGF-Beta 3 ELISA Kit PicoKine®Rat TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf4-ox40-picokine-trade-elisa-kit-ek1106-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1106.pngHuman TNFRSF4/OX40 ELISA Kit PicoKine®Human TNFRSF4/OX40 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cystatin-c-picokine-trade-elisa-kit-ek1109-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1109.jpgRat Cystatin C/CST3 ELISA Kit PicoKine®Rat Cystatin C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-fasl-picokine-trade-elisa-kit-ek1110-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1110.pngRat FASL ELISA Kit PicoKine®Rat FASL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-galectin-9-picokine-trade-elisa-kit-ek1113-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1113.jpgHuman Galectin-9 / Gal 9 ELISA Kit PicoKine®Human Galectin-9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-granzyme-b-picokine-trade-elisa-kit-ek1114-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1114.pngHuman Granzyme B ELISA Kit PicoKine®Human Granzyme B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il1rl1-st2-picokine-trade-elisa-kit-ek1116-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1116_1.pngHuman IL-1 RL1 / ST2 ELISA Kit PicoKine®Human IL1RL1/ST2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-elafin-pi3-picokine-trade-elisa-kit-ek1117-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1117.pngHuman Elafin/PI3 ELISA Kit PicoKine®Human Elafin/PI3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-a2m-alpha2-macroglobulin-picokine-trade-elisa-kit-ek1118-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1118.jpgHuman A2M/Alpha 2-Macroglobulin ELISA Kit PicoKine®Human A2M/alpha2-Macroglobulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-thrombomodulin-picokine-trade-elisa-kit-ek1119-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1119.pngMouse Thrombomodulin ELISA Kit PicoKine®Mouse Thrombomodulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl15-picokine-trade-elisa-kit-ek1120-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1120.pngHuman CCL15/Mip 1 Delta ELISA Kit PicoKine®Human CCL15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf13c-baffr-picokine-trade-elisa-kit-ek1121-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1121.jpgHuman TNFRSF13C/BAFFR ELISA Kit PicoKine®Human TNFRSF13C/BAFFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf13c-baffr-picokine-trade-elisa-kit-ek1122-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1122.jpgMouse TNFRSF13C/BAFFR ELISA Kit PicoKine®Mouse TNFRSF13C/BAFFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl14-hcc-1-picokine-trade-elisa-kit-ek1123-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1123.pngHuman CCL14/HCC-1 ELISA Kit PicoKine®Human CCL14/HCC-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl6-c10-picokine-trade-elisa-kit-ek1125-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1125.pngMouse CCL6/C10 ELISA Kit PicoKine®Mouse CCL6/C10 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl16-hcc-4-picokine-trade-elisa-kit-ek1127-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1127.jpgHuman CCL16/HCC-4 ELISA Kit PicoKine®Human CCL16/HCC-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl12-mcp5-picokine-trade-elisa-kit-ek1128-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1128.pngMouse CCL12/MCP5 ELISA Kit PicoKine®Mouse CCL12/MCP5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tlr1-picokine-trade-elisa-kit-ek1129-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1129.pngHuman TLR1/TIL ELISA Kit PicoKine®Human TLR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tlr2-picokine-trade-elisa-kit-ek1130-boster.html2026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1130.pngMouse TLR2/Toll-like receptor 2 ELISA Kit PicoKine®Mouse TLR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-gdf-15-picokine-trade-elisa-kit-ek1134-boster.html2026-03-27T05:07:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1134.pngMouse GDF-15 ELISA Kit PicoKine®Mouse GDF-15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-17f-picokine-trade-elisa-kit-ek1136-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1136.pngRat IL-17F/Interleukin-17F ELISA Kit PicoKine®Rat IL-17F PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl8-mcp-2-picokine-trade-elisa-kit-ek1137-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1137.pngMouse CCL8/MCP-2 ELISA Kit PicoKine®Mouse CCL8/MCP2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-nesfatin-1-picokine-trade-elisa-kit-ek1138-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1138_1.pngHuman Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®Human Nesfatin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pon1-picokine-trade-elisa-kit-ek1141-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1141_1.pngHuman PON1/Paraoxonase 1 ELISA Kit PicoKine®Human PON1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il15ra-picokine-trade-elisa-kit-ek1142-boster.html2026-03-24T05:03:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1142.jpgMouse IL15RA ELISA Kit PicoKine®Mouse IL15RA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf9-4-1bb-picokine-trade-elisa-kit-ek1143-boster.html2026-04-01T05:01:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1143.pngMouse TNFRSF9/4-1BB ELISA Kit PicoKine®Mouse TNFRSF9/4-1BB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf13b-taci-picokine-trade-elisa-kit-ek1144-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1144.jpgMouse TNFRSF13B/TACI ELISA Kit PicoKine®Mouse TNFRSF13B/TACI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl25-teck-picokine-trade-elisa-kit-ek1145-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1145.pngHuman CCL25/TECK ELISA Kit PicoKine®Human CCL25/TECK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd163-picokine-trade-elisa-kit-ek1146-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1146.jpgMouse CD163 ELISA Kit PicoKine®Mouse CD163 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pcsk9-picokine-trade-elisa-kit-ek1147-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1147.pngHuman PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine®Human PCSK9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-pcsk9-picokine-trade-elisa-kit-ek1148-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1148.pngMouse PCSK9/Proprotein Convertase 9 ELISA Kit PicoKine®Mouse PCSK9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fgf19-picokine-trade-elisa-kit-ek1160-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1160.pngHuman FGF19 ELISA Kit PicoKine®Human FGF19 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-azurocidin-picokine-trade-elisa-kit-ek1161-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1161.pngHuman Azurocidin ELISA Kit PicoKine®Human Azurocidin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-fgf1-picokine-trade-elisa-kit-ek1172-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1172.pngMouse FGF1/Fgf Acidic ELISA Kit PicoKine®Mouse FGF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-fgf1-picokine-trade-elisa-kit-ek1173-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1173.pngRat FGF1/Fgf Acidic ELISA Kit PicoKine®Rat FGF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfsf12-picokine-trade-elisa-kit-ek1176-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1176_2.pngMouse TNFSF12/Tweak ELISA Kit PicoKine®Mouse TNFSF12/TWEAK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-tnfsf12-picokine-trade-elisa-kit-ek1177-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1177.pngRat TNFSF12/Tweak ELISA Kit PicoKine®Rat TNFSF12/TWEAK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-sclerostin-sost-picokine-trade-elisa-kit-ek1179-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1179_1.pngMouse Sclerostin/SOST ELISA Kit PicoKine®Mouse Sclerostin/SOST PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il1rl1-st2-picokine-trade-elisa-kit-ek1180-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1180.pngMouse IL-1 RL1 / ST2 ELISA Kit PicoKine®Mouse IL1RL1/ST2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-17rc-picokine-trade-elisa-kit-ek1181-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1181.pngMouse IL-17RC ELISA Kit PicoKine®Mouse IL-17RC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-igfbp5-picokine-trade-elisa-kit-ek1183-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1183.pngMouse IGFBP5 ELISA Kit PicoKine®Mouse IGFBP5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd200-picokine-trade-elisa-kit-ek1185-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1185.pngMouse CD200 ELISA Kit PicoKine®Mouse CD200 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-sp-d-picokine-trade-elisa-kit-ek1186-boster.html2026-04-01T05:01:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1186_1.jpgMouse SP-D ELISA Kit PicoKine®Mouse SP-D PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-periostin-osf2-picokine-trade-elisa-kit-ek1187-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1187.jpgMouse Periostin/OSF2 ELISA Kit PicoKine®Mouse Periostin/OSF2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ace2-picokine-trade-elisa-kit-ek1188-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1188.pngMouse ACE2 ELISA Kit PicoKine®Mouse ACE2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-msp-mst1-picokine-trade-elisa-kit-ek1189-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1189.pngMouse MSP/MST1 ELISA Kit PicoKine®Mouse MSP/MST1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-saa-picokine-trade-elisa-kit-ek1190-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1190_2.pngMouse SAA/SAA1 ELISA Kit PicoKine®Mouse SAA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-progranulin-picokine-trade-elisa-kit-ek1192-boster.html2026-03-24T05:03:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1192.jpgMouse Progranulin ELISA Kit PicoKine®Mouse Progranulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-betacellulin-btc-picokine-trade-elisa-kit-ek1193-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1193.jpgMouse Betacellulin/BTC ELISA Kit PicoKine®Mouse Betacellulin/BTC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mfge8-lactadherin-picokine-trade-elisa-kit-ek1194-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1194.pngMouse MFGE8 / Lactadherin / Milk Fat Globule 1 ELISA Kit PicoKine®Mouse MFGE8/Lactadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-wisp1-ccn4-picokine-trade-elisa-kit-ek1195-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1195.pngMouse WISP1/CCN4 ELISA Kit PicoKine®Mouse WISP1/CCN4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-gas6-picokine-trade-elisa-kit-ek1196-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1196.pngMouse GAS6 ELISA Kit PicoKine®Mouse GAS6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cfd-picokine-trade-elisa-kit-ek1199-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1199.jpgHuman CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®Human CFD PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lifr-picokine-trade-elisa-kit-ek1200-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1200.pngHuman LIFR/Lif R Alpha ELISA Kit PicoKine®Human LIFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mfge8-lactadherin-picokine-trade-elisa-kit-ek1201-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1201_5.pngHuman MFGE8/Lactadherin ELISA Kit PicoKine®Human MFGE8/Lactadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tgfbi-beta-ig-h3-picokine-trade-elisa-kit-ek1202-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1202.pngHuman beta IG-H3/TGFBI ELISA Kit PicoKine®Human TGFBI/beta IG-H3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cyr61-ccn1-picokine-trade-elisa-kit-ek1203-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1203_1.pngHuman CYR61/CCN1 ELISA Kit PicoKine®Human CYR61/CCN1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-wisp1-ccn4-picokine-trade-elisa-kit-ek1204-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1204.pngHuman WISP1/CCN4 ELISA Kit PicoKine®Human WISP1/CCN4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cystatin-b-picokine-trade-elisa-kit-ek1205-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1205.pngHuman Cystatin B/CSTB ELISA Kit PicoKine®Human Cystatin B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tslp-picokine-trade-elisa-kit-ek1206-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1206.pngMouse TSLP ELISA Kit PicoKine®Mouse TSLP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-psp94-picokine-trade-elisa-kit-ek1207-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1207_1.pngHuman PSP94/MSMB ELISA Kit PicoKine®Human PSP94 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-sap-ptx2-picokine-trade-elisa-kit-ek1208-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1208.jpgMouse SAP/PTX2/APCS ELISA Kit PicoKine®Mouse SAP/PTX2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-sap-ptx2-picokine-trade-elisa-kit-ek1209-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1209.pngRat SAP/PTX2/APCS ELISA Kit PicoKine®Rat SAP/PTX2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sparc-picokine-trade-elisa-kit-ek1210-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1210.jpgHuman SPARC ELISA Kit PicoKine®Human SPARC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-upar-picokine-trade-elisa-kit-ek1215-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1215.pngMouse uPAR / uPA Receptor ELISA Kit PicoKine®Mouse uPAR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd13-aminopeptidase-n-picokine-trade-elisa-kit-ek1216-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1216.jpgHuman CD13/Aminopeptidase N ELISA Kit PicoKine®Human CD13/Aminopeptidase N PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lap-tgf-beta1-picokine-trade-elisa-kit-ek1218-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1218.pngHuman LAP(TGF-Beta1) ELISA Kit PicoKine®Human LAP(TGF-beta1) PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-mip-1alpha-ccl3-picokine-trade-elisa-kit-ek1219-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1219.jpgRat MIP-1Alpha/CCL3 ELISA Kit PicoKine®Rat MIP-1alpha/CCL3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-soluble-cd5-picokine-trade-elisa-kit-ek1220-boster.html2026-03-24T05:03:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1220.pngHuman soluble CD5 ELISA Kit PicoKine®Human soluble CD5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-soluble-cd6-picokine-trade-elisa-kit-ek1221-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1221.pngHuman soluble CD6 ELISA Kit PicoKine®Human soluble CD6 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd13-aminopeptidase-n-picokine-trade-elisa-kit-ek1222-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1222.jpgMouse CD13/Aminopeptidase N ELISA Kit PicoKine®Mouse CD13/Aminopeptidase N PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd56-ncam1-picokine-trade-elisa-kit-ek1223-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1223.jpgMouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine®Mouse CD56/NCAM1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl23-mpif-1-picokine-trade-elisa-kit-ek1224-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1224.pngHuman CCL23/MPIF-1 ELISA Kit PicoKine®Human CCL23/MPIF-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl9-picokine-trade-elisa-kit-ek1225-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1225.jpgMouse CCL9/MIP-1 gamma ELISA Kit PicoKine®Mouse CCL9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf14-hvem-picokine-trade-elisa-kit-ek1226-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1226.pngHuman TNFRSF14/HVEM ELISA Kit PicoKine®Human TNFRSF14/HVEM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf14-hvem-picokine-trade-elisa-kit-ek1227-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1227.pngMouse TNFRSF14/HVEM ELISA Kit PicoKine®Mouse TNFRSF14/HVEM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-jam-a-f11r-picokine-trade-elisa-kit-ek1228-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1228.pngMouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®Mouse JAM-A/F11R PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-relaxin-1-picokine-trade-elisa-kit-ek1231-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1231.jpgMouse Relaxin 1 ELISA Kit PicoKine®Mouse Relaxin 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tff1-picokine-trade-elisa-kit-ek1232-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1232_1.pngHuman TFF1/PS2 ELISA Kit PicoKine®Human TFF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tff2-picokine-trade-elisa-kit-ek1233-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1233_1.pngHuman TFF2/Trefoil factor 2 ELISA Kit PicoKine®Human TFF2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tff3-picokine-trade-elisa-kit-ek1234-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1234_1.pngHuman TFF3/ITF ELISA Kit PicoKine®Human TFF3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-midkine-picokine-trade-elisa-kit-ek1235-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1235.jpgHuman Midkine ELISA Kit PicoKine®Human Midkine PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-spink1-tati-picokine-trade-elisa-kit-ek1241-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1241.pngHuman SPINK1/TATI ELISA Kit PicoKine®Human SPINK1/TATI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-folr1-picokine-trade-elisa-kit-ek1242-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1242.jpgHuman FOLR1/Alpha Fr ELISA Kit PicoKine®Human FOLR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-folr1-picokine-trade-elisa-kit-ek1243-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1243.jpgMouse FOLR1/Alpha Fr ELISA Kit PicoKine®Mouse FOLR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lumican-picokine-trade-elisa-kit-ek1244-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1244.pngHuman Lumican ELISA Kit PicoKine®Human Lumican PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-renin-picokine-trade-elisa-kit-ek1249-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1249.pngHuman Renin / Prorenin ELISA Kit PicoKine®Human Renin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-renin-1-picokine-trade-elisa-kit-ek1250-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1250_1.pngMouse Renin 1/Ren1 ELISA Kit PicoKine®Mouse Renin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-gpnmb-osteoactivin-picokine-trade-elisa-kit-ek1251-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1251.pngHuman GPNMB/Osteoactivin ELISA Kit PicoKine®Human GPNMB/Osteoactivin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tetranectin-clec3b-picokine-trade-elisa-kit-ek1253-boster.html2026-03-24T05:03:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1253.jpgHuman Tetranectin/CLEC3B ELISA Kit PicoKine®Human Tetranectin/CLEC3B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-thioredoxin-picokine-trade-elisa-kit-ek1254-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1254_1.pngHuman Thioredoxin ELISA Kit PicoKine®Human Thioredoxin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd320-8d6a-picokine-trade-elisa-kit-ek1255-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1255.pngHuman CD320 / 8D6A / TCblR ELISA Kit PicoKine®Human CD320/8D6A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ficolin-1-picokine-trade-elisa-kit-ek1257-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1257.jpgHuman Ficolin-1 / FCN1 / M Ficolin ELISA Kit PicoKine®Human Ficolin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ficolin-2-picokine-trade-elisa-kit-ek1258-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1258.jpgHuman Ficolin-2 ELISA Kit PicoKine®Human Ficolin-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ficolin-3-picokine-trade-elisa-kit-ek1259-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1259.jpgHuman Ficolin-3 ELISA Kit PicoKine®Human Ficolin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-18r1-picokine-trade-elisa-kit-ek1260-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1260.pngHuman IL-18R1 ELISA Kit PicoKine®Human IL-18R1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-lumican-picokine-trade-elisa-kit-ek1261-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1261_1.pngMouse Lumican ELISA Kit PicoKine®Mouse Lumican PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-lumican-picokine-trade-elisa-kit-ek1262-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1262_1.pngRat Lumican ELISA Kit PicoKine®Rat Lumican PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-prostasin-picokine-trade-elisa-kit-ek1272-boster.html2026-03-24T05:03:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1272.pngHuman Prostasin ELISA Kit PicoKine®Human Prostasin 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ADAM12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-adam12-picokine-trade-elisa-kit-ek1294-boster.html2026-03-24T05:03:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1294.pngMouse ADAM12 ELISA Kit PicoKine®Mouse ADAM12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-angiopoietin-1-picokine-trade-elisa-kit-ek1295-boster.html2026-03-24T05:03:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1295.pngRat Angiopoietin-1 ELISA Kit PicoKine®Rat Angiopoietin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-angiopoietin-1-picokine-trade-elisa-kit-ek1296-boster.html2026-03-24T05:03:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1296.pngMouse Angiopoietin-1 ELISA Kit PicoKine®Mouse Angiopoietin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-btc-picokine-trade-elisa-kit-ek1297-boster.html2026-03-24T05:03:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1297.jpgRat Betacellulin/BTC ELISA Kit PicoKine®Rat BTC PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-igfbp5-picokine-trade-elisa-kit-ek1298-boster.html2026-03-24T05:03:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1298.pngRat IGFBP5 ELISA Kit PicoKine®Rat IGFBP5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cd36-sr-b3-picokine-trade-elisa-kit-ek1299-boster.html2026-03-24T05:03:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1299_1.pngRat CD36/SR-B3/GP4 ELISA Kit PicoKine®Rat CD36/SR-B3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-hgf-picokine-trade-elisa-kit-ek1301-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1301.pngRat HGF/Hepatocyte growth factor ELISA Kit PicoKine®Rat HGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-timp-2-picokine-trade-elisa-kit-ek1302-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1302_1.pngRat TIMP-2 ELISA Kit PicoKine®Rat TIMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd22-picokine-trade-elisa-kit-ek1303-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1303.pngMouse CD22/Siglec 2 ELISA Kit PicoKine®Mouse CD22 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd169-siglec-1-picokine-trade-elisa-kit-ek1304-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1304_3.pngHuman CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine®Human CD169/SIGLEC-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd169-siglec-1-picokine-trade-elisa-kit-ek1305-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1305.pngMouse CD169/SIGLEC-1/Sialoadhesin ELISA Kit PicoKine®Mouse CD169/SIGLEC-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-e-picokine-trade-elisa-kit-ek1306-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1306_2.pngHuman Cathepsin E ELISA Kit PicoKine®Human Cathepsin E PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tgfbr2-picokine-trade-elisa-kit-ek1307-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1307.pngHuman TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine®Human TGFBR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfsf18-gitrl-picokine-trade-elisa-kit-ek1308-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1308.pngMouse TNFSF18/GITRL ELISA Kit PicoKine®Mouse TNFSF18/GITRL PicoKine ELISA Kit standard curve 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PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd21-cr2-picokine-trade-elisa-kit-ek1315-boster.html2026-03-24T05:03:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1315.pngHuman CD21/CR2 ELISA Kit PicoKine®Human CD21/CR2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-crp-picokine-trade-elisa-kit-ek1316-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1316.jpgHuman CRP/C Reactive Protein ELISA Kit PicoKine®Human CRP PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calponin-antibody-monoclonal-ma1011-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1011-41598_2016_article_bfsrep30230_fig3_html.jpgAnti-Calponin Cnn2 Antibody (Monoclonal, CP-93)MRJP1 expression changed mouse VSMC protoeme setting. (a) Clustered heatmap displayed differentially expressed proteins between control and MRJP1 expressing mouse VSMCs with three replications in each group. (b) The expressions of αSMA, SM22, calponin and PCNA in MRJP1 expressing VSMCs are significantly reduced by Western blotting analysis compared with controls, the band intensities were normalized to GAPDH (n = 3, mean ± S.D) from three independent experiments. * p < 0.05, ** p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep30230'>27444336</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1011-calponin-primary-antibodies-wb-testing-1.jpgAnti-Calponin Cnn2 Antibody (Monoclonal, CP-93) Western blot analysis of Calponin/CNN2 using anti-Calponin/CNN2 antibody (MA1011). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: chicken heart tissue lysates,<br> Lane 2: chicken heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Calponin/CNN2 antigen affinity purified monoclonal antibody (Catalog # MA1011) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calponin/CNN2 at approximately 33 kDa. The expected band size for Calponin/CNN2 is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-catenin-gamma-plakoglobin-antibody-monoclonal-ma1012-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1012-catenin-gamma-primary-antibodies-wb-testing-1.jpgAnti-Catenin Gamma (Plakoglobin) Jup Antibody (Monoclonal, 15F11) Western blot analysis of Catenin gamma(Plakoglobin) using anti-Catenin gamma(Plakoglobin) antibody (MA1012). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates, <br> Lane 5: human CACO-2 whole cell lysates, <br> Lane 6: human K562 whole cell lysates, <br> Lane 7: human 293T whole cell lysates, <br> Lane 8: human PC-3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Catenin gamma(Plakoglobin) antigen affinity purified monoclonal antibody (Catalog # MA1012) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Catenin gamma(Plakoglobin) at approximately 82 kDa. The expected band size for Catenin gamma(Plakoglobin) is at 82 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-d-antibody-monoclonal-ma1013-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1013-1-IHC-anti-cathepsin-d-antibody.jpgAnti-Cathepsin D Ctsd Monoclonal Antibody IHC analysis of Cathepsin D using anti-Cathepsin D antibody (MA1013). <br> Cathepsin D was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Cathepsin D Antibody (MA1013) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1013-2-IHC-anti-cathepsin-d-antibody.jpgAnti-Cathepsin D Ctsd Monoclonal Antibody IHC analysis of Cathepsin D using anti-Cathepsin D antibody (MA1013). <br> Cathepsin D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Cathepsin D Antibody (MA1013) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1013-3-IHC-anti-cathepsin-d-antibody.jpgAnti-Cathepsin D Ctsd Monoclonal Antibody IHC analysis of Cathepsin D using anti-Cathepsin D antibody (MA1013). <br> Cathepsin D was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Cathepsin D Antibody (MA1013) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1013-ctsd-primary-antibodies-wb-testing-4.jpgAnti-Cathepsin D Ctsd Monoclonal Antibody Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (MA1013). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human HepG2 whole cell lysates, <br> Lane 6: human A431 whole cell lysates, <br> Lane 7: human MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cathepsin D antigen affinity purified monoclonal antibody (Catalog # MA1013) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 28 kDa. The expected band size for Cathepsin D is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-l-antibody-monoclonal-ma1014-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1014-ctsl-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin L Ctsl Antibody (Monoclonal, 33/2) Western blot analysis of Cathepsin L using anti-Cathepsin L antibody (MA1014). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cathepsin L antigen affinity purified monoclonal antibody (Catalog # MA1014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cathepsin L at approximately 25 kDa. The expected band size for Cathepsin L is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd3-antibody-monoclonal-ma1015-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1015-cd3_-primary-antibodies-fc-testing-1.jpgAnti-CD3 CD3E Antibody (Monoclonal, CA-3) Flow Cytometry analysis of human PBMC cells using anti-CD3 antibody (MA1015). <br>Overlay histogram showing human PBMC cells stained with MA1015 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD3 Antibody (MA1015&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse gG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd4-antibody-monoclonal-ma1016-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1016-cd4-primary-antibodies-fc-testing-1.jpgAnti-CD4 Antibody (Monoclonal, CA-4) Flow Cytometry analysis of human PBMC cells using anti-CD4 antibody (MA1016). <br>Overlay histogram showing human PBMC cells stained with MA1016 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD4 Antibody (MA1016&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd8-antibody-monoclonal-ma1017-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1017-cd8-primary-antibodies-fc-testing-1.jpgAnti-CD8 Cd8A Antibody (Monoclonal, CA-8) Flow Cytometry analysis of human PBMC cells using anti-CD8 antibody (MA1017). <br>Overlay histogram showing human PBMC cells stained with MA1017 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD8 Antibody (MA1017&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1017-cd8-primary-antibodies-wb-testing-2.jpgAnti-CD8 Cd8A Antibody (Monoclonal, CA-8) IHC analysis of CD8 using anti-CD8 antibody (MA1017). <br> CD8 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD8 Antibody (MA1017) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc25c-antibody-monoclonal-ma1018-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-CDC25C Antibody (Monoclonal, DCS-193)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc6-antibody-monoclonal-ma1019-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-CDC6 Antibody (Monoclonal, DCS-180)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk4-antibody-monoclonal-ma1020-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1020-cdk4-primary-antibodies-wb-testing-1.jpgAnti-CDK4 Antibody (Monoclonal, DCS-31) Western blot analysis of CDK4 using anti-CDK4 antibody (MA1020). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: rat PC-12 whole cell lysates, <br> Lane 4: mosue NIH/3T3 whole cell lysates, <br> Lane 5: mosue RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK4 antigen affinity purified monoclonal antibody (Catalog # MA1020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk6-antibody-monoclonal-ma1021-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1021-cdk6-primary-antibodies-wb-testing-1.jpgAnti-CDK6 Antibody (Monoclonal, DCS-90) Western blot analysis of CDK6 using anti-CDK6 antibody (MA1021). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK6 antigen affinity purified monoclonal antibody (Catalog # MA1021) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CDK6 at approximately 37 kDa. The expected band size for CDK6 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk7-cak-antibody-monoclonal-ma1022-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1022.jpgAnti-Cdk7/CAK Antibody (Monoclonal, MO-1.1)Anti-Cdk7/CAK antibody (monoclonal)&#44; MA1022&#44; IHC(P)<br> IHC(P): Human Breast Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ceacam5-cd66e-antibody-monoclonal-ma1023-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1023-1-WB-anti-ceacam1-cd66a-antibody.jpgAnti-CEACAM5/Cd66e Monoclonal Antibody Western blot analysis of CEACAM5/Cd66e using anti-CEACAM5/Cd66e antibody (MA1023). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Recombinant Human CEACAM5/Cd66e Protein 10ng<br> Lane 2; Recombinant Human CEACAM5/Cd66e Protein 5ng<br> Lane 3: Recombinant Human CEACAM5/Cd66e Protein 2.5ng<br> Lane 4: Recombinant Human CEACAM5/Cd66e Protein 1.25ng <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CEACAM5/Cd66e antigen affinity purified monoclonal antibody (Catalog # MA1023) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1023-cd66e-primary-antibodies-ihc-testing-2_1.jpgAnti-CEACAM5/Cd66e Monoclonal Antibody IHC analysis of CEACAM5/Cd66e using anti-CEACAM5/Cd66e antibody (MA1023). <br> CEACAM5/Cd66e was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CEACAM5/Cd66e Antibody (MA1023) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1023-cd66e-primary-antibodies-ihc-testing-3_2.jpgAnti-CEACAM5/Cd66e Monoclonal Antibody IHC analysis of CEACAM5/Cd66e using anti-CEACAM5/Cd66e antibody (MA1023). <br> CEACAM5/Cd66e was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with Antibody Diluent overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1023-cd66e-primary-antibodies-ihc-testing-4_1_1.jpgAnti-CEACAM5/Cd66e Monoclonal Antibody IHC analysis of CEACAM5/Cd66e using anti-CEACAM5/Cd66e antibody (MA1023). <br> CEACAM5/Cd66e was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CEACAM5/Cd66e Antibody (MA1023) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-peptide-7-antibody-monoclonal-ma1024-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Cytokeratin Peptide 7 KRT7 Antibody (Monoclonal, LDS-68)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-peptide-18-antibody-monoclonal-ma1026-boster.html2026-04-01T05:01:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1026-krt18-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) Western blot analysis of Cytokeratin Peptide 18 using anti-Cytokeratin Peptide 18 antibody (MA1026). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytokeratin Peptide 18 antigen affinity purified monoclonal antibody (Catalog # MA1026) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cytokeratin Peptide 18 at approximately 48 kDa. The expected band size for Cytokeratin Peptide 18 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1026-2-IHC-anti-cytokeratin-peptide-18-antibody-monoclonal-cy-90.jpgAnti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) IHC analysis of Cytokeratin Peptide 18 using anti-Cytokeratin Peptide 18 antibody (MA1026). <br> Cytokeratin Peptide 18 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Cytokeratin Peptide 18 Antibody (MA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1026-3-IHC-anti-cytokeratin-peptide-18-antibody-monoclonal-cy-90.jpgAnti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) IF analysis of Cytokeratin Peptide 18 using anti-Cytokeratin Peptide 18 antibody (MA1026). <br> Cytokeratin Peptide 18 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Cytokeratin Peptide 18 Antibody (MA1026) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1026-4-IHC-anti-cytokeratin-peptide-18-antibody-monoclonal-cy-90.jpgAnti-Cytokeratin Peptide 18 KRT18 Antibody (Monoclonal, CY-90) IHC analysis of Cytokeratin Peptide 18 using anti-Cytokeratin Peptide 18 antibody (MA1026). <br> Cytokeratin Peptide 18 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Cytokeratin Peptide 18 Antibody (MA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-myc-antibody-monoclonal-ma1028-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-41419_2021_4348_fig3_html.pngAnti-c-Myc Antibody (Monoclonal, 9E10)L1-70 is associated with Top1. A Co-immunoprecipitation of the hippocampus tissue homogenates from 5- to 7-day-old wild-type mice with an antibody against L1 cytoplasmic domain being used for the co-immunoprecipitation and antibody against Top1 being used for western blotting. B ELISA for measuring the interaction between L1-70 and Top1 which were prepared from genetically engineered bacteria expressing recombinant L1-70-Myc and Top1-HA. Antibodies against Myc and HA tags were used for the identification of L1-70 protein and Top1 protein. The absorbance of L1-70-myc increased with the higher concentration of Top1-HA and showed saturation at 1 nM. C Co-expression of L1-70 and Top1 in primary cultured cells from the hippocampus of wild-type newborn mice. Immunofluorescence staining for L1-70 and Top1. L1-70 (green) colocalized with Top1 (red) in the nucleus. D Proximity ligation assay (Duolink) of the interaction between endogenous L1-70 protein and Top1 protein in primary hippocampal neurons from wild-type newborn mice. L1-70/Top1 complexes are indicated by the red dots. E Proximity ligation assay (Duolink) of the interaction between recombinant L1-70-Myc protein and Top1-HA protein transduced in U87 cells (which lack expression of L1) with recombinant adeno-associated virus expressing L1-70-Myc or/and Top1-HA. Antibodies against Myc and HA tags were used in the Duolink analysis. Three groups were transduced with recombinant viruses, AAV-Top1-HA, AAV-L1-70-Myc, or both. Positive signals could be found only in U87 cells transduced with both adeno-associated viruses. Scale bar: 20 µm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04348-6'>35013124</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-41419_2021_4348_fig4_html.pngAnti-c-Myc Antibody (Monoclonal, 9E10)The L1-70/Top1 complex regulates MIF expression in hippocampal neurons. A Western blot analysis of the cell lysates of neuroblastoma N2a cells treated with tacrine. Antibodies against L1 cytoplasmic domain, against MIF and against β-actin were used for immunoblotting. Expression of L1 and L1-70 was induced by tacrine and accompanied by an increase in MIF expression level. B Relative full-length L1, L1-70, and MIF levels were calculated and normalized to β-actin. The data represent means ± SD, * p < 0.05, ** p < 0.01, **** p < 0.0001, one-way ANOVA with Tukey’s post hoc test, three independent experiments. C U87 cells were transduced with lentivirus, encoding a MIF promoter-driven EGFP, for 48 h. Then, AAV-L1-70-Myc , AAV-Top1-HA , and both AAV-L1-70-Myc and AAV-Top1-HA were added. After 36 h, a strong EGFP signal could be found in cells transduced with both AAV-L1-70-Myc and AAV-Top1-HA . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04348-6'>35013124</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-41598_2018_36985_fig1_html.pngAnti-c-Myc Antibody (Monoclonal, 9E10)Comparison of the expression of lncRNA PVT1 and c-myc in GC and normal tissues by ISH and IHC. ( A ) Comparison of the expression of lncRNA PVT1 in GC and normal tissues by TMA and ISH. PVT1 staining was stronger in GC tissues. (a) PVT1 staining in Han GC tissues (40×); (b) PVT1 staining in Han GC tissues (200×, 400× in the lower right corner); (c) PVT1 staining in Han normal gastric tissues (40×); (d) PVT1 staining in Han normal gastric tissues (200×, 400× in the lower right corner); (e) PVT1 staining in Uygur GC tissues (40×); (f) PVT1 staining in Uygur GC tissues (200×, 400× in the lower right corner); (g) PVT1 staining in Uygur normal gastric tissues (40×); (h) PVT1 staining in Uygur normal gastric tissues (200×, 400× in the lower right corner). ( B ) Comparison of c-myc expression in GC and normal tissues by TMA and IHC. Staining of c-myc was stronger in GC tissues. (a) c-myc staining in Han GC tissues (40×); (b) c-myc staining in Han GC tissues (200×, 400× in the lower right corner); (c) c-myc staining in Han normal gastric tissues (40×; (d) c-myc staining in Han normal gastric tissues (200×, 400× in the lower right corner); (e) c-myc staining in Uygur GC tissues (40×); (f) c-myc staining in Uygur GC tissues (200×, 400× in the lower right corner); (g) c-myc staining in Uygur normal gastric tissues (40×); (h) c-myc staining in Uygur normal gastric tissues (200×, 400× in the lower right corner). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-36985-x'>30679629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-41598_2018_36985_fig2_html.pngAnti-c-Myc Antibody (Monoclonal, 9E10)Decreased c-myc expression in BGC823 and AGS cells after interference with PVT1 expression. ( A ) Real time-PCR results show the endogenous PVT1 expression levels in the six GC cell lines BGC823, MGC803, MKN45, SGC7901, AGS and N87. ( B ) RNAi was used to interfere with the expression of PVT1 in BGC823 and AGS cells, and then the efficiencies of PVT1 knockdown were investigated using real time-PCR. ( C , D ) Detection of c-myc protein expression levels by western blotting in BGC823 and AGS cells after silencing of PVT1. * P < 0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-36985-x'>30679629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-c-myc-primary-antibodies-wb-testing-1.jpgAnti-c-Myc Antibody (Monoclonal, 9E10) Western blot analysis of c-Myc using anti-c-Myc antibody (MA1028). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-c-Myc antigen affinity purified monoclonal antibody (Catalog # MA1028) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for c-Myc at approximately 49 kDa. The expected band size for c-Myc is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-c-myc-primary-antibodies-ihc-testing-2.jpgAnti-c-Myc Antibody (Monoclonal, 9E10) IHC analysis of c-Myc using anti-c-Myc antibody (MA1028).<br> c-Myc was detected in paraffin-embedded section of human spleen tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-c-Myc Antibody (MA1028) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-c-myc-primary-antibodies-ihc-testing-3.jpgAnti-c-Myc Antibody (Monoclonal, 9E10) IHC analysis of c-Myc using anti-c-Myc antibody (MA1028).<br> c-Myc was detected in paraffin-embedded section of human colorectal adenocarcinoma tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-c-Myc Antibody (MA1028) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1028-c-myc-primary-antibodies-ihc-testing-4.jpgAnti-c-Myc Antibody (Monoclonal, 9E10) IHC analysis of c-Myc using anti-c-Myc antibody (MA1028).<br> c-Myc was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/ml mouse anti-c-Myc Antibody (MA1028) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-type-iii-antibody-monoclonal-ma1029-boster.html2026-03-24T05:03:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1029-1-WB-anti-collagen-type-iii-antibody-monoclonal-fh-7a.jpgAnti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A)Anti-Collagen&#44; Type III antibody (monoclonal)&#44; MA1029&#44; Western blotting<br>Lane 1: HT1080 Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br><br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1029-2-IHC-anti-collagen-type-iii-antibody-monoclonal-fh-7a.jpgAnti-Collagen, Type III Col3a1 Antibody (Monoclonal, FH-7A)Anti-Collagen&#44; Type III antibody (monoclonal)&#44; MA1029&#44; IHC(F)<br>IHC(F): Mouse Kidney Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-a-antibody-monoclonal-ma1032-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1032-ccna2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) Western blot analysis of Cyclin A using anti-Cyclin A antibody (MA1032). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclin A antigen affinity purified monoclonal antibody (Catalog # MA1032) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cyclin A at approximately 55 kDa. The expected band size for Cyclin A is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1032-13578_2022_744_fig4_html.pngAnti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)AIF blocks hypoxia-induced progression of PH in vitro and in vivo. A Hypoxia increased the viability of PASMCs after growth arrest for 24 h, and this effect was decreased by AIF (n = 4). B Pretreatment with an AIF overexpression plasmid blocked the effects of hypoxia on EdU incorporation in cells (n = 6). Scale bars: 50 μm. C Cell cycle analysis by flow cytometry indicated that hypoxia stimulated cell progression into G 2 /M + S phase, and this effect was inhibited by AIF overexpression (n = 3). D Effects of AIF on the expression of PCNA, Cyclin A and Cyclin D under hypoxia (n = 4–5). E Represents weight ratio of the right ventricular (RV)/left ventricular (LV) + Septum (n = 6); F Represents the right ventricular systolic pressure (RVSP) from mice (n = 5); G pulmonary artery velocity time integral (PAVTI), pulmonary artery acceleration time (PAAT) and left ventricular ejection fraction (LVEF) of the hypoxic mouse model infected with AAV5-NC and AAV5-AIF (n = 6). All data are presented as the means ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001; Nor normoxia, Hyp hypoxia, NC negative control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-022-00744-3'>35090552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1032-2-IF-anti-cyclin-a-antibody-monoclonal-cy-a1.jpgAnti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032). <br> Cyclin A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1032-3-IF-anti-cyclin-a-antibody-monoclonal-cy-a1.jpgAnti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032). <br> Cyclin A was detected in an immunocytochemical section of SMMC-7721 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-d2-antibody-monoclonal-ma1034-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Cyclin D2 Ccnd2 Antibody (Monoclonal, DCS-3)Boster Kit Box https://www.bosterbio.com/products/anti-desmin-antibody-monoclonal-m01948-4-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01948-4-desmin-primary-antibodies-wb-testing-1.jpgAnti-Desmin Antibody (Monoclonal, 35D56)Western blot analysis of Desmin using anti-Desmin antibody (M01948-4). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat skeletal muscle tissue lysates,<br> Lane 3: rat C2C12 whole cell lysates.<br> Lane 4: mouse heart tissue lysates,<br> Lane 5: mouse skeletal muscle tissue lysates.<br> After Electrophoresis, proteins were tDesminsferred to a Nitrocellulose membDesmine at 150mA for 50-90 minutes. Blocked the membDesmine with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membDesmine was incubated with mouse anti-Desmin antigen affinity purified monoclonal antibody (Catalog # M01948-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-elastin-antibody-monoclonal-ma1038-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Elastin ELN Antibody (Monoclonal, BA-4)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-episialin-ema-antibody-monoclonal-ma1039-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1039-3-IHC-anti-episialin-ema-antibody-monoclonal-gp1-4.jpgAnti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4)Anti-Episialin&#44; EMA antibody&#44; MA1039&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1039-1-IHC-anti-episialin-ema-antibody-monoclonal-gp1-4.jpgAnti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4)Anti-Episialin&#44; EMA antibody&#44; MA1039&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1039-2-IHC-anti-episialin-ema-antibody-monoclonal-gp1-4.jpgAnti-Episialin, EMA MUC1 Antibody (Monoclonal, GP1.4)Anti-Episialin&#44; EMA antibody&#44; MA1039&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegfr2-antibody-monoclonal-ma1040-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-VEGFR2/KDR Monoclonal AntibodyBoster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-filensin-antibody-monoclonal-ma1041-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Filensin Bfsp1 Antibody (Monoclonal, FIL-7B10)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gap43-antibody-monoclonal-ma1042-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1042-1-IHC-anti-gap43-antibody-monoclonal-gap-7b10.jpgAnti-GAP43 Antibody (Monoclonal, GAP-7B10)Anti-GAP43 antibody&#44; MA1042&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gelsolin-antibody-monoclonal-ma1044-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1044-1-IHC-anti-gelsolin-antibody-monoclonal-gs-2c4.jpgAnti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4)Anti-Gelsolin antibody&#44; MA1044&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1044-2-IHC-anti-gelsolin-antibody-monoclonal-gs-2c4.jpgAnti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4)Anti-Gelsolin antibody&#44; MA1044&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1044-3.jpgAnti-Gelsolin Gsn Antibody (Monoclonal, GS-2C4) Western blot analysis of Gelsolin using anti-Gelsolin antibody (MA1044). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysate&#44; <br> Lane 2: human A549 whole cell lysate&#44; <br> Lane 3: monkey COS-7 whole cell lysate&#44; <br> Lane 4: human Caco-2 whole cell lysate&#44; <br> Lane 5: human HepG2 whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Gelsolin antigen affinity purified monoclonal antibody (Catalog # MA1044) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 90KD. The expected band size for Gelsolin is at 86KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gfap-antibody-monoclonal-ma1045-boster.html2026-03-24T05:03:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-gfap-primary-antibodies-wb-testing-1.jpgAnti-GFAP Antibody (Monoclonal, G-A-5) Western blot analysis of GFAP using anti-GFAP antibody (MA1045). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: rat C6 whole cell lysates, <br> Lane 4: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GFAP antigen affinity purified monoclonal antibody (Catalog # MA1045) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-2.jpgAnti-GFAP Antibody (Monoclonal, G-A-5) IF analysis of GFAP using anti-GFAP antibody (MA1045) and anti-MBP antibody (PA1050) <br> GFAP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti-GFAP Antibody (MA1045)and anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-12951_2023_2217_fig3_html.pngAnti-GFAP Antibody (Monoclonal, G-A-5)EF-sEVs promoted neurogenesis at the injury site in the spinal cord. A Representative immunofluorescence images showing the staining of neurofilaments (NF, green) and glial fibrillary acidic protein (GFAP, red) in lesion sites in different groups. B Fluorescent immunostaining of choline acetyl transferase (ChAT, green) in lesion sites in different groups. Scale bar=500 μm ( A , B left line before magnification) and 100 μm ( A , B right lines after magnification) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-023-02217-2'>38012570</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-ad-14-5-1853-g3.jpgAnti-GFAP Antibody (Monoclonal, G-A-5)CNTN1 overexpression by AAV stereotactic injection activated microglia and astrocyte in the hippocampus. (A-C) Representative images showed immunostaining against microglial marker Iba1 on brain sections in the hippocampus of mice in different groups. (D&E) Quantiative analysis of Iba1 positive microglia of hippocampus in different groups by total number of microglia (D) and integrated optical density (IOD) (E). (F) Quantitative analysis complexity of Iba1 positive microglia in hippocampus with Sholl analysis in different groups. (G) Quantitative real time qPCR detection of mRNA expression levels of CD11b and CD68 in hippocampus in different groups. (H) Quantitative real time qPCR detection of mRNA expression levels of IL1α, IL6, iNOS and CCL2 in hippocampus in different groups. n=4 per group. (I&J) Representative immunoblot (I) and quantitative analysis (J) of vimentin and GFAP expression in hippocampus in different groups. Data in , E, H, J were analyzed by Kruskal-Wallis statistical test; Data in was analyzed by two-way ANOVA followed by Bonferroni’s multiple comparison test. Data in was analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison tests. Data were presented as mean ± sem. *p < 0.05, **p < 0.01 and ****p < 0.0001. Scale bar: 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10529752/&orig_host=www.ncbi.nlm.nih.gov'>37196127</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-ad-14-5-1853-g5.jpgAnti-GFAP Antibody (Monoclonal, G-A-5)Minocycline inhibited microglia activation and the following astrocyte activation induced by hippocampal CNTN1 overexpression. (A-F) Representative images showed immunostaining against microglial marker Iba1 on brain sections in hippocampus of mice in different groups. (G) Quantiative analysis of Iba1 positive microglia in hippocampus of mice in different groups by integrated optical density (IOD). (H) Quantitative real time qPCR detection of mRNA expression of CD11b and CD68 in hippocampus. (I) Quantitative analyses of complexity with Iba1 positive microglia in hippocampus in different groups. (J) Quantitative real time qPCR detection of mRNA expression of IL1α, IL6 and CCL2 in hippocampus in different groups. n=6 per group. (K&L) Representative immunoblots (K) and quantitative analyses (L) of vimentin and GFAP expression in hippocampus in different groups. Data in , and L were analyzed by Kruskal-Wallis statistical test; Data in . I was analyzed by two-way ANOVA followed by Bonferroni’s multiple comparison tests. Data in , I and J were analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison tests. Data were presented as mean ± sem. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. NS: Saline. Scale bar: 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10529752/&orig_host=www.ncbi.nlm.nih.gov'>37196127</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-12868_2022_756_fig3_html.pngAnti-GFAP Antibody (Monoclonal, G-A-5)CIH induced activation of astrocytes. a GFAP immunohistochemical staining and GFAP + cell count in each subregion of the hippocampus. b Sholl analysis of morphological changes of astrocytes under CIH. Scale bar = 50 μm. Data are presented as the mean ± SD. ANOVA with Tukey’s post hoc test was used to compare the control, CIH 2w, CIH 4w, and CIH 6w group. There were three rat brain slices in each group, and 5–6 visual fields were randomly selected according to different subregions for statistical analysis. * P < 0.05, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12868-022-00756-2'>36437451</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-12868_2022_756_fig4_html.pngAnti-GFAP Antibody (Monoclonal, G-A-5)CIH induced A1/A2 phenotype astrocyte transformation, with the increase of A1 type. a Immunofluorescence images of astrocytes. The astrocytes were labeled by anti-GFAP (green), anti-C3d (red), and anti-S100a10 (red). b Expression of GFAP, C3d, and S100a10 was detected by Western blotting. c The mRNA expression of GFAP, C3d, and S100a10. Scale bar = 50 μm. Data are presented as the mean ± SD. ANOVA with Tukey’s post hoc test was used to compare the control, CIH 2w, CIH 4w, and CIH 6w groups. For RT-qPCR experiments, each group consisted of 4 rats, and the experiment was repeated 3 times; for western blotting, which was repeated 4 times, there were 5 rats per group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12868-022-00756-2'>36437451</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-12868_2022_756_fig7_html.pngAnti-GFAP Antibody (Monoclonal, G-A-5)Inhibition of NLRP3 inflammasome reversed CIH-related A1/A2 astrocyte phenotypic transformation. a GFAP immunohistochemical staining and GFAP + cell count. b Sholl analysis of morphological changes of astrocytes. c-d Expression of GFAP, C3d, and s100a10 detected by Western blotting and RT-qPCR. Scale bar = 50 μm. Data are presented as the mean ± SD. For RT-qPCR experiments, each group consisted of 4 rats, and the experiment was repeated 3 times; for Western blotting, the experiment was repeated 4 times and there were 5 rats per group. For statistical analysis, an unpaired t-test was used. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12868-022-00756-2'>36437451</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-fnagi-14-883503-g0007.jpgAnti-GFAP Antibody (Monoclonal, G-A-5)Effects of MSCs treatment on the activation of microglia and astrocytes. (A,D) Representative images of immunofluorescence staining of Iba-1 and GFAP in the cortex and hippocampus. (B,C,E,F) Quantification of immunofluorescence staining for Iba-1 and GFPAP in the cortex and hippocampus (* P < 0.05, ** P < 0.01, vs. sham-operated group; # P < 0.05, ## P < 0.01, vs. RHRSP group; n = 6/group). scale bar = 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9160459/&orig_host=www.ncbi.nlm.nih.gov'>35663575</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-fnagi-14-883503-g0008.jpgAnti-GFAP Antibody (Monoclonal, G-A-5)Effects of MSCs treatment on the phenotype distribution of microglia and astrocytes. (A–D) Double immunofluorescence staining of Iba-1/iNOS-positive M1 microglia, Iba-1/Arg-1-positive M2 microglia, GFAP/C3-positive A1 astrocytes and GFAP/S100A10-positive A2 astrocytes in hippocampus. (E–H) Representative quantification showing that MSC significantly decreased the number of M1, A1 cells and increased the number of M2, A2 cells in hippocampus. (** P < 0.01, vs. Sham-operated group, ## P < 0.01, vs. RHRSP group; n = 6/group). scale bar = 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9160459/&orig_host=www.ncbi.nlm.nih.gov'>35663575</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-fnins-16-813472-g002.jpgAnti-GFAP Antibody (Monoclonal, G-A-5)Vagus nerve stimulation (VNS) decreases spinal cord tissue damage. (A–C) Representative Immunofluorescence (IF) staining images of each group post injure 28 days, and bar charts show the fluorescence intensity mean value (IMV) for GFAP and Laminin, n = 5 per group, scale bar = 500 or 200 μm. (D,E) Representative Hematoxylin-eosin (HE) staining images 28 days after injury and quantification data of cavity necrotic tissue in each group, n = 5 per group, scale bar = 500 μm. (F,G) Representative Nissl staining images 28 days after injury and quantification of the numbers of Nissl-stained dark neurons in each group, n = 5 per group, scale bar = 20 μm. * P < 0.05, ** P < 0.01, and **** P < 0.0001 between the Sham and Sham-VNS groups, # P < 0.05, ## P < 0.01 between the VNS and VNS-MLA groups.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9022634/&orig_host=www.ncbi.nlm.nih.gov'>35464311</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-41419_2019_1596_fig1_html.pngAnti-GFAP Antibody (Monoclonal, G-A-5)ALK5 is increased in the ischemic hemisphere in a MCAO/R rat model. a Representative images of ALK5 expression in the ischemic hemisphere 24 h after I/R ( n = 6 biological replicates). b Representative images of ALK5 expression in the ischemic hemisphere at 14 d after I/R ( n = 6 biological replicates). c Comparison of mean intensity ratios in Western blot analysis. d Representative images of immunohistochemical staining for ALK5 expression 24 h and 14 d after I/R ( n = 5 biological replicates) (scale bar = 100 μm). e Comparison of the mean density value in immunohistochemical analysis for ALK5 expression. f Representative images of immunofluorescence staining for ALK5 (green), beta-III tubulin (red)/GFAP (red) and cellular nuclei (blue). (scale bar = 100 μm). Arrows show the positive cells, and the inserted images show magnified images of representative cells. * P < 0.05, compared to the sham group at the same time point (Student’s t test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-019-1596-z'>31043581</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-12974_2025_3554_fig4_html.pngAnti-GFAP Antibody (Monoclonal, G-A-5)CCK8 inhibits C1q-mediated microglial phagocytosis of synapses and A1 astrocyte polarisation in SAE model mice. (A) Experimental timeline. (B) Venn diagram and heatmap showing differentially expressed genes in the control and LPS + CCK8 groups compared with the LPS group. (C) mRNA and (D, E) protein levels of C1q were detected by qPCR and western blot, respectively. Immunofluorescence was used to detect the number of PSD95 + C1q + with microglia (F, J), Iba1 fluorescence intensity (G), soma volume (H), branch length (I) and the colocalization of (K, L) GFAP with C3 in the dorsal hippocampal CA1 region of mice. (M, N) Western blot was used to detect GFAP and C3 protein levels in the hippocampus of mice. Data are expressed as the mean ± SD ( n = 4–5 per group). All data were analysed by two-way ANOVA followed by Bonferroni post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. LPS group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-025-03554-9'>41024113</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1045-1-IHC-anti-gfap-antibody-monoclonal-g-a-5.jpgAnti-GFAP Antibody (Monoclonal, G-A-5) IHC analysis of GFAP using anti-GFAP antibody (MA1045). <br> GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-GFAP Antibody (MA1045) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-gfap-primary-antibodies-icc-testing-1.jpgAnti-GFAP Antibody (Monoclonal, G-A-5)IF analysis of GFAP using anti-GFAP antibody (MA1045) . <br> GFAP was detected in an immunocytochemical section of rat C6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with mouse anti-GFAP Antibody (MA1045) at a dilution of 2 μg/mL overnight at 4°C. DyLight®488 Conjugated Goat Anti-mouse IgG (BA1126) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1045-gfap-primary-antibodies-if-testing-1.pngAnti-GFAP Antibody (Monoclonal, G-A-5)IF analysis of GFAP using anti-GFAP antibody (MA1045).<br> GFAP was detected in a paraffin-embedded section of rat spinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GFAP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glucagon-antibody-monoclonal-ma1047-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1047-glucagon-primary-antibodies-ihc-testing-1.jpgAnti-Glucagon Gcg Antibody (Monoclonal, K79bB10)IHC analysis of Glucagon using anti-Glucagon antibody (MA1047). <br> Glucagon was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1047-glucagon-primary-antibodies-ihc-testing-2.jpgAnti-Glucagon Gcg Antibody (Monoclonal, K79bB10)IHC analysis of Glucagon using anti-Glucagon antibody (MA1047). <br> Glucagon was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1047-fphar-12-688073-g004.jpgAnti-Glucagon Gcg Antibody (Monoclonal, K79bB10)Effects of the RBPP-P on islet histology. (A) Immunofluorescence (IF) staining for insulin (green) and glucagon (red). Scale bar, 100 μm. (B) β-cell area/pancreatic area ratio, measured by immunofluorescence staining ( n = 5). (C) Insulin levels in serum ( n = 10). All data are expressed as mean ± s. d. # p < 0.05, vs. C57BL/6J mice; *p < 0.05, vs. ALX mice.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.688073/full'>34262457</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1047-glucagon-primary-antibodies-if-testing-2.jpgAnti-Glucagon Gcg Antibody (Monoclonal, K79bB10) IF analysis of Glucagon using anti-Glucagon antibody (MA1047). <br> Glucagon was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL mouse anti-Glucagon Antibody (MA1047) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp27-antibody-monoclonal-ma1048-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-HSP27 Hspb1 Monoclonal AntibodyBoster Kit Box https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-hsp60-antibody-monoclonal-ma1049-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1049-hsp60-primary-antibodies-wb-testing-1.jpgAnti-HSP60 Hspd1 Antibody (Monoclonal, LK1) Western blot analysis of HSP60 using anti-HSP60 antibody (MA1049). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human U87 whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: mouse Neuro-2a whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP60 antigen affinity purified monoclonal antibody (Catalog # MA1049) at 2 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP60 at approximately 65 kDa. The expected band size for HSP60 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp70-antibody-monoclonal-ma1050-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1050-hsp70-primary-antibodies-wb-testing-1.jpgAnti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) Western blot analysis of HSP70 using anti-HSP70 antibody (MA1050). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human U20S whole cell lysates, <br> Lane 5: rat lung tissue lysates, <br> Lane 6: rat liver tissue lysates, <br> Lane 7: mouse lung tissue lysates, <br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP70 antigen affinity purified monoclonal antibody (Catalog # MA1050) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1050-hsp70-primary-antibodies-ihc-testing-2.jpgAnti-HSP70 Hspa1b Antibody (Monoclonal, BRM-22) IHC analysis of HSP70 using anti-HSP70 antibody (MA1050).<br> HSP70 was detected in paraffin-embedded section of human breast cancer tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-HSP70 Antibody (MA1050) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-insulin-antibody-monoclonal-ma1052-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1052-1-IHC-anti-insulin-antibody-monoclonal-k36ac10.jpgAnti-Insulin Ins2 Antibody (Monoclonal, K36AC10)Anti-Insulin antibody (monoclonal)&#44; MA1052&#44; IHC(P)<br>IHC(P): Rat Pancreas Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1052-2-IHC-anti-insulin-antibody-monoclonal-k36ac10.jpgAnti-Insulin Ins2 Antibody (Monoclonal, K36AC10)Anti-Insulin antibody (monoclonal)&#44; MA1052&#44; IHC(P)<br>IHC(P): Rat Pancreas Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1052-12986_2010_article_257_fig3_html.jpgAnti-Insulin Ins2 Antibody (Monoclonal, K36AC10)HE Staining (×400 magnification) and insulin immunofluorescence staining (×200 magnification) in a rat pancreatic islet . Islet cells in the NC (A1 and B1) and CUGFR (A2 and B2) groups 4 weeks after catch-up growth with HE staining (A1 and A2) and insulin fluorescence staining (B1 and B2). Green fluorescence shows cells positive for insulin. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1743-7075-7-45'>20504302</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1052-12986_2010_article_257_fig4_html.jpgAnti-Insulin Ins2 Antibody (Monoclonal, K36AC10)Effect of catch-up growth on beta cell relative area and mass . After insulin staining of rat pancreatic islet, the area positively stained for insulin was quantified by Image Pro Plus software. Values are means ± SD (n = 6). *P < 0.05 versus NC group. **P < 0.01 versus NC group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1743-7075-7-45'>20504302</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1052-12986_2010_article_257_fig5_html.jpgAnti-Insulin Ins2 Antibody (Monoclonal, K36AC10)Effect of catch-up growth on islet function and GLP-1 concentration . Plasma glucose (A), insulin (B), GLP-1(C) levels and AUC Insulin , AUC Glucose , AUC GLP-1 during a 1-hour OGTT, which was conducted 0 (A1, B1 and C1), 2 (A2, B2 and C2) and 4 (A3, B3 and C3) weeks after catch-up growth in the NC (red) and CUGFR (green) groups, were measured. Data are expressed as means ± SD of six rats in each group. *P ≤ 0.05 versus NC group; **P ≤ 0.01 versus NC group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1743-7075-7-45'>20504302</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1052-12986_2010_article_257_fig7_html.jpgAnti-Insulin Ins2 Antibody (Monoclonal, K36AC10)Time course of the glucose and insulin following oral and IV glucose in the normal and catch-up growth rats . Plasma glucose (A1, A2, A3) and insulin (B1, B2, B3) concentrations in rats of NC group (red) or CUGFR group (green) during oral glucose tolerance test (solid line) and intravenous glucose infusion test (broken lines) designed to match the oral glucose curve in six rats, were detected after catch up growth for 0 (A1, B1), 2(A2, B2) and 4 (A3, B3) weeks. Data are expressed as means ± SD. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1743-7075-7-45'>20504302</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1052-12986_2010_article_257_fig8_html.jpgAnti-Insulin Ins2 Antibody (Monoclonal, K36AC10)Effect of catch-up growth on the incretin effect . Data are expressed as means ± SD. The incretin effect was calculated by comparing the insulin response to oral and matched IV glucose load. * P ≤ 0.05 versus NC group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1743-7075-7-45'>20504302</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-involucrin-antibody-monoclonal-ma1053-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1053-involucrin-primary-antibodies-wb-testing-1.jpgAnti-Involucrin Ivl Antibody (Monoclonal, SY5) Western blot analysis of Involucrin using anti-Involucrin antibody (MA1053). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates, <br> Lane 2: human T-47D whole cell lysates, <br> Lane 3: human A431 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Involucrin antigen affinity purified monoclonal antibody (Catalog # MA1053) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Involucrin at approximately 68 kDa. The expected band size for Involucrin is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1053-1-IHC-anti-involucrin-antibody-monoclonal-sy5.jpgAnti-Involucrin Ivl Antibody (Monoclonal, SY5) IHC analysis of Involucrin using anti-Involucrin antibody (MA1053). <br> Involucrin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Involucrin Antibody (MA1053) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-laminin-antibody-monoclonal-ma1054-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1054-laminin-primary-antibodies-ihc-testing-1.jpgAnti-Laminin LAMA1 Monoclonal Antibody IHC analysis of Laminin using anti-Laminin antibody (MA1054). <br> Laminin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Laminin Antibody (MA1054) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-map-kinase-activated-diphosphorylated-erk-1-2-antibody-monoclonal-ma1055-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1055-mapk1-primary-antibodies-wb-testing-1.jpgAnti-Phospho-MAP Kinase, Activated(Diphosphorylated ERK-1&2) Mapk3 Antibody (Monoclonal, MAPK-YT) Western blot analysis of MAP kinase,activated using anti-MAP kinase,activated antibody (MA1055). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-MAP kinase,activated antigen affinity purified monoclonal antibody (Catalog # MA1055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for MAP kinase,activated at approximately 42 kDa. The expected band size for MAP kinase,activated is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-map2-antibody-monoclonal-ma1057-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1057-map2-primary-antibodies-wb-testing-1.jpgAnti-MAP2 Antibody (Monoclonal, HM-2) Western blot analysis of MAP2 using anti-MAP2 antibody (MA1057). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAP2 antigen affinity purified monoclonal antibody (MA1057) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP2 at approximately 280 kDa. The expected band size for MAP2 is at 200 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1057-1-IHC-anti-map2-antibody-monoclonal-hm-2.jpgAnti-MAP2 Antibody (Monoclonal, HM-2)Anti-MAP2 antibody (monoclonal)&#44; MA1057&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1057-fphar-16-1554945-g011.jpgAnti-MAP2 Antibody (Monoclonal, HM-2)TUDCA promoted neuron regeneration along endogenous NSCs migration at day 7 after SCI. (A) Co-immunofluorescence showed endogenous NSCs (Nestin, green) and reactive astrocytes (GFAP, red) at the margin of the lesion site at day 7 after SCI. (B) Endogenous NSCs (Nestin, green) and neuron (NeuN, red) at the margin of the lesion site at day 7 after SCI. (C, D) Quantitative polymerase chain reaction (qPCR) showing the expression of Nestin, GFAP, NeuN, MAP2 and Oligo 2 at day 7 after SCI. All experiments were performed in triplicated and data were presented means ± SEM, n = 3 per group. *P < 0.05, **P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1057-fpsyt-09-00776-g002.jpgAnti-MAP2 Antibody (Monoclonal, HM-2)Granule cell morphology and synaptic integrity changes between anxiety-like and depression-like behaviors. (A,B) Representative Golgi-Cox staining images of dendritic spines and spine density in the hippocampal DG granule cells in CRS-treated rats, CFA-treated rats, and the controls. n = 10 for each group, bar = 10 μm. (C) Representative images of Golgi-Cox -stained granule cells in the DG area (top) and the reconstruction of its dendritic branches (bottom) from each group. Bar = 50 μm. (D) There were no significant effects of CRS and CFA injection on the dendritic length of the DG granule cells. (E) Sholl analysis of dendritic length in DG granule cells. CRS reduced dendrite intersection in the region 285–345 mm away from the soma compared with the control group. A repeated measures analysis of variance (ANOVA), * p < 0.05 vs. CON, n = 10 for each group; all graphs represent mean ± SEM. (F,G) Expression of SYP and MAP2 in the hippocampus was evaluated by Western blotting. Semi-quantitative analyses of SYP and MAP2 expression were performed. Note that the expression of SYP was significantly reduced in CRS-treated rats but was increased in CFA -treated rats. DG: dentate gyrus; CFA: complete Freund's adjuvant; CRS, chronic restraint stress; SYP, synaptophysin; MAP2, microtubule-associated protein 2. * p < 0.05, vs. control group (one-way ANOVA). All of the data are presented as mean ± SEM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/psychiatry/articles/10.3389/fpsyt.2018.00776/full'>30740068</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1057-41467_2018_5209_fig4_html.pngAnti-MAP2 Antibody (Monoclonal, HM-2)In vitro differentiation potential of Ptf1a-reprogrammed miNSCs. a Schematic showing that miNSCs reprogrammed directly from MEFs by Ptf1a are able to differentiate into neurons, astrocytes, and oligodendrocytes under proper culture conditions. b miNSC10 cells underwent drastic morphological changes to form neuron-like cells when the culture medium was switched from NSC medium to neural differentiation medium. c miNSC10 cells could be differentiated into neurons immunoreactive for Tuj1, Map2, Dcx, NeuN, Tau, Peripherin, or GABA. They were also capable of differentiating into astrocytes (immunoreactive for GFAP) and oligodendrocytes (immunoreactive for O1, CNP, or MBP). d In vitro differentiated neurons were immunoreactive for both Tuj1 and synapsin. Cells in c and d were counterstained with nuclear DAPI. e Quantification of Map2 + neurons, GFAP+ astrocytes, and O1+ oligodendrocytes differentiated from miNSC10 cells under different differentiation conditions. f A merged micrograph showing a typical GFP-tagged neuron differentiated from miNSCs that was chosen for patch-clamp recording. g Voltage-clamp recordings indicated fast activated and inactivated inward sodium currents as well as outward potassium currents on a differentiated neuron. h Current-clamp recordings revealed action potential responses of the differentiated neuron under current injection. i Multiple action potentials were induced after depolarization of the neuron. j Spontaneous postsynaptic currents recorded from an in vitro differentiated neuron. Scale bars, 80 μm ( b ) and 40 μm ( c , d ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-018-05209-1'>30030434</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1057-41467_2018_5209_fig9_html.pngAnti-MAP2 Antibody (Monoclonal, HM-2)Ptf1a reprograms human foreskin fibroblasts (HFF) into tripotent iNSCs. a , b Ectopic expression of Ptf1a in HFFs by lentiviruses induced the formation of neurospheres. c , d In the absence of doxycycline (Dox), Ptf1a-induced neurosphere cells were capable of forming neurospheres before passage 20 ( c ), but lost the capacity after passage 20 and became monolayered ( d ). e – i Ptf1a-induced human neural stem cells (hiNSCs) were highly immunoreactive for PTF1A, SOX2, PAX6, NESTIN, OLIG2, and FABP7. j – p Ptf1a-induced hiNSCs were capable of differentiating into neurons immunoreactive for TUJ1, MAP2, NEUN, TAU, or GABA, astrocytes labeled by GFAP, or oligodendrocytes marked by O1. q Neurons differentiated from hiNSCs were immunoreactive for both Tuj1 and synapsin. Cells in f – q were counterstained with nuclear DAPI. r Voltage-clamp recordings indicated fast activated and inactivated inward sodium currents as well as outward potassium currents on a differentiated neuron. s Current-clamp recordings revealed action potential responses of a differentiated neuron under current injection. t An action potential was induced after depolarization of the neuron. u Spontaneous postsynaptic currents recorded from a differentiated neuron. Scale bars, 80 μm ( a – d ) and 40 μm ( e – q ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-018-05209-1'>30030434</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1057-nrr-8-427-g003.jpgAnti-MAP2 Antibody (Monoclonal, HM-2)Overall expression of nestin, NSE, MAP2, GFAP, TH and VMAT2 in induced rat bone marrow-derived mesenchymal stem cells under different conditions. Expression rate (%) = number of positive cells/total number of cells × 100%. Data were determined by immunocytochemistry and expressed as mean ± SEM. Statistical differences were tested using F -test. a P < 0.05, vs . control group; b P < 0.05, vs . Xiangdan injection group and ATRA + GDNF group. NSE: Neuron-specific enolase; MAP2: microtubule- associated protein 2; GFAP: glial fibrillary acid protein; TH: tyrosine hydroxylase; VMAT2: vesicular monoamine transporter-2; group A: Xiangdan injection; group B: all-trans retinoic acid + glial cell line-derived neurotrophic factor; group C: sonic hedgehog + fibroblast growth factor 8.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4146134/&orig_host=www.ncbi.nlm.nih.gov'>25206684</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm7-antibody-monoclonal-ma1058-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Mcm7 Antibody (Monoclonal, DCS-141)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p-glycoprotein-mdr-antibody-monoclonal-ma1060-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1060-abcb1-primary-antibodies-wb-testing-1.jpgAnti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4) Western blot analysis of ABCB1 using anti-ABCB1 antibody (MA1060). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human DLD1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ABCB1 antigen affinity purified monoclonal antibody (Catalog # MA1060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for ABCB1 at approximately 200 kDa. The expected band size for ABCB1 is at 141 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mucin-gastric-antibody-monoclonal-ma1061-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1061-mucin-gastric-primary-antibodies-ihc-testing-1.jpgAnti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061). <br> Mucin Gastric was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1061-mucin-gastric-primary-antibodies-ihc-testing-2.jpgAnti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061). <br> Mucin Gastric was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1061-mucin-gastric-primary-antibodies-ihc-testing-3.jpgAnti-Mucin Gastric MUC5AC Antibody (Monoclonal, 45M1) IHC analysis of Mucin Gastric using anti-Mucin Gastric antibody (MA1061). <br> Mucin Gastric was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Mucin Gastric Antibody (MA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myoglobin-antibody-monoclonal-ma1062-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1062-myoglobin-primary-antibodies-ihc-testing-1.jpgAnti-Myoglobin Mb Antibody (Monoclonal, MG-1)IHC analysis of Myoglobin using anti-Myoglobin antibody (MA1062). <br> Myoglobin was detected in a paraffin-embedded section of human tongue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Myoglobin Antibody (MA1062) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myosin-skeletal-fast-antibody-monoclonal-ma1063-boster.html2026-03-24T05:03:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1063-1-WB-anti-myosin-skeletal-fast-antibody-monoclonal-my-32.jpgAnti-Myosin(Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32)Anti-Myosin(Skeletal&#44; Fast) antibody (monoclonal)&#44; MA1063&#44; Western blotting<br>WB: Rat Skeletal Muscle Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1063-41419_2015_article_bfcddis2015225_fig5_html.jpgAnti-Myosin(Skeletal, Fast) MYH2 Antibody (Monoclonal, MY-32)miR-411-5p activate p38MAPK pathway by targeting SPRY4-induced terminal differentiation of RMS. ( a ) Western blot analysis of p38MAPK activation (P-p38MAPK: p-38) in RMS cells treated with HA-tagged MKK6EE protein-expressing pcDNA3, SPRY4 siRNA, or both. ( b ) Western blot analysis of p38MAPK activation (P-p38MAPK:p-38) in RMS cells treated with miR-411-5p-M, miR-411-5p-I, and MKK6EE protein-expressing pcDNA3 plus miR-411-5p-M. ( c ) Terminal differentiation of RD cells after treatment with SPRY4 siRNA or miR-411-5p-M for 6 and 48 h. Cells stained with apoptosis marker (caspase-3); cell cycle was analyzed using propidium iodide (PI) and showed changed morphology (MHC and myosin) after activation of p38MAPK caused by SPRY4 siRNA or miR-411-5p-M. Scale bars: panel (Caspase-3)=100 μ m, panel (MHC and Myosin)=20 μ m. ( d ) Quantitation of terminal differentiation of RD cells after treatment with SPRY4 siRNA or miR-411-5p-M for 6 and 48 h. ( e ) Graphical representation of cell cycle phase proportions in RD cells after treatment with miR-411-5p-M for 6 and 48 h. ( f ) Proliferation of RD cells was evaluated by [ 3 H] thymidine incorporation assay after treatment with SPRY4 siRNA or miR-411-5p-M for 4 days. The amount of [ 3 H] thymidine incorporated was measured with a liquid scintillation counter. The OD at 557 nm was determined using a microplate reader. ( g ) Western blot analysis of cleaved caspase-3 in RMS cells treated with SPRY4 siRNA or miR-411-5p-M for 24 and 72 h. Each assay ( a – g ) was conducted at least three times independently. Error bars indicate S.D. * P <0.05; ** P <0.01; *** P <0.005 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis2015225'>26291313</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nebulin-antibody-monoclonal-ma1069-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1069-1-WB-anti-nebulin-antibody-monoclonal-nb2.jpgAnti-Nebulin Antibody (Monoclonal, NB2)Anti-Nebulin antibody&#44; MA1069&#44; Western blotting<br>All lanes: Anti Nebulin (MA1069) at 0.5ug/ml<br> WB: Mouse Skeletal Muscle Tissue Lysate at 50ug<br> Predicted bind size: 773KD<br> Observed bind size: 773KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nf68-antibody-monoclonal-ma1070-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1070-nf68-primary-antibodies-wb-testing-1.jpgAnti-NF68 Nefl Antibody (Monoclonal, NR4) Western blot analysis of NF68 using anti-NF68 antibody (MA1070). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates, <br> Lane 2: human U251 whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NF68 antigen affinity purified monoclonal antibody (Catalog # MA1070) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NF68 at approximately 72 kDa. The expected band size for NF68 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1070-nf68-primary-antibodies-ihc-testing-2.jpgAnti-NF68 Nefl Antibody (Monoclonal, NR4) IHC analysis of NF68 using anti-NF68 antibody (MA1070). <br> NF68 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NF68 Antibody (MA1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1070-nf68-primary-antibodies-ihc-testing-3.jpgAnti-NF68 Nefl Antibody (Monoclonal, NR4) IHC analysis of NF68 using anti-NF68 antibody (MA1070). <br> NF68 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-NF68 Antibody (MA1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nefh-antibody-monoclonal-ma1071-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1071-1_1-IHC-anti-nefh-antibody-monoclonal-n52.jpgAnti-NEFH Antibody (Monoclonal, N52) IHC analysis of NEFH using anti-NEFH antibody (MA1071).<br> NEFH was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NEFH Antibody (MA1071) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-research.0676.fig.002.jpgAnti-NEFH Antibody (Monoclonal, N52)LIPUS improves cerebral WM integrity on day 28 after stroke. (A) Schematic of the localization of the peri-infarct cortex (CTX), external capsule (EC), and striatum (STR). (B) Double-labeled immunofluorescence staining of MBP (green) and SMI32 (red) in 3 brain regions, CTX, EC, and STR, on day 28 after dMCAO (i). Quantification of MBP immunoreactivity (ii) and the SMI32/MBP fluorescence intensity ratio (iii) are presented as the ratio of fold change compared to the Sham control, scale bar = 50 μm, n = 6 mice/group. (C) Representative images depicting that the Western blot analysis of MBPs was obtained for each group (i). The expression levels of the target proteins were normalized relative to β-actin (ii). (D) Representative immunostaining of MBP and SMI32 on day 28 after dMCAO (i), scale bar = 200 μm; (ii) quantification of the ratio of MBP to SMI32 staining-positive areas in ipsilateral injured brain tissue. Data were normalized to stained areas in the contralateral hemisphere, n = 6 mice/group. (E) Representative images of myelin coverage (i), calculated MBP+ myelin coverage area along NF200+ nerve fibers (ii), scale bar = 20 μm, n = 6 mice/group. Data are presented as means ± SD. Statistical analysis for (B) was conducted using 2-way ANOVA and then Tukey’s multiple comparisons test, while (C) and (E) were analyzed using one-way ANOVA and then Tukey’s multiple comparisons test. Analysis for (D) involved unpaired 2-tailed Student’s t test. * P < 0.05, ** P < 0.01, *** P < 0.001,**** P < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12022504/'>40290135</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-ejh-68-4-4137-g005.jpgAnti-NEFH Antibody (Monoclonal, N52)CR immunolabeling in the cochlea at P8. A,B ) A low-magnification view of the cross-sections of the cochlea labeled with CR (green) at P8; the expression of CR in the IHCs and OHCs was observed in the apical turns; nerve fibers within the cochlear modiolus were immunoreactive for CR; many CR-positive SGNs (arrowheads) were membranous and round-shaped. C ) Dual-labeling of CR (green) with efferent terminals marker synaptophysin (red) in the P8 organ of Corti; presynaptic synaptophysin spots underneath the IHCs did not coexpressed with CR-labelled afferent nerve fibers, CR-labeled afferent terminals (arrowheads) was seen in contact with the IHCs. D-F ) Dual-labeling of CR (green) with afferent terminals marker NF200 (red) in the P8 organ of Corti; CR-labelled nerve fibres (arrowheads) innervating the pillar side of the IHC were coexpressed with NF200. G-I ) Double labeling with CR (green) and NF200 (red) in the P8 osseous spiral lamina and the merged image + DAPI; CR was colocalized with NF200 in the P8 osseous spiral lamina. GER, greater epithelial ridge; IHC, inner hair cell; OHC, outer hair cell; SGN, spiral ganglion neuron; CM, cochlear modiolus; CP, cuticular plates; OSL, osseous spiral lamina.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11583137/'>39508782</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-ejh-68-4-4137-g006.jpgAnti-NEFH Antibody (Monoclonal, N52)A,B ) Detail of CR (green) and phalloidin (red) labeling in the middle turn of P14 cochlea; CR was detectable in the P14 IHC and OHC, colocalization of CR with phalloidin was found in the cuticular plates of the hair cells; CR-positive cochlear neural fibres (arrows) preferentially contacted the pillar side of the IHC. C ) CR immunolabeling in the peripheral neurites innervating the IHC was also observed in the basal turn of P14. D-F ) Double labeling with CR (green) and NF200 (red) in the P14 osseous spiral lamina and the merged image + DAPI; CR was colocalized with NF200 in the P14 osseous spiral lamina. G-I ) Double labeling with CR (green) and NF200 (red) expression in the P14 SGNs and the merged image + DAPI; CR immunolabeling was present in the plasma membrane of SGNs; NF200-positive the cytoplasm of SGNs appeared surrounded by CR-positive the plasma membrane of SGNs. IHC, inner hair cell; OHC, outer hair cell; SGN, spiral ganglion neuron; OSL, osseous spiral lamina; SB, spiral limbus.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11583137/'>39508782</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-ejh-68-4-4137-g007.jpgAnti-NEFH Antibody (Monoclonal, N52)A-C ) Detail of CR (green) and NF200 (red) labeling in the middle turn of adult cochlea; afferent terminals (arrows) innervating the IHCs are double positive for CR (green) and NF200 (red). D-F ) Double labeling with CR (green) and NF200 (red) in the adult osseous spiral lamina and the merged image + DAPI. CR was colocalized with NF200 in the adult osseous spiral lamina. G-I ) Double labeling with CR (green) and NF200 (red) expression in the adult SGNs and the merged image + DAPI; CR immunolabeling was present in the plasma membrane of adult SGNs. CR-positive the plasma membrane of SGNs was not colocalized with NF200-positive the cytoplasm of SGNs. J-L ) Double labeling with CR (red) and peripherin (green) in the adult SGNs and the merged image + DAPI; CR was not colocalized with peripherin in the adult SGNs. IHC, inner hair cell; OHC, outer hair cell; SGN, spiral ganglion neuron; OSL, osseous spiral lamina.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11583137/'>39508782</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-ejh-68-4-4137-g008.jpgAnti-NEFH Antibody (Monoclonal, N52)A ) Detail of peripherin (green) immunolabeling in the P1 cochlea; peripherin-positive type II cochlear afferent nerve fibers were found below the IHCs and OHC. B,C ) Double labeling with peripherin (green) and TUJ1 (red) in the P1 SGNs and the merged image + DAPI; type II SGNs was negative for peripherin. D,E ) Peripherin-positive type II cochlear afferent nerve fibers innervated mainly three rows of OHC. F ) Type II SGNs of P5 were immunoreactive for peripherin. G) Peripherin-positive type II cochlear afferent nerve fibers projected beyond the floor of the tunnel of Corti to the OHCs. H,I ) Type II SGNs of P8 were immunoreactive for peripherin, peripherin-immunoreactive type II SGNs did not colocalize with TUJ1-labelled SGNs. G,K,L ) Double labeling with peripherin (green) and NF200 (red) in the adult SGNs and the merged image + DAPI; peripherin was colocalized with NF200 in the adult SGNs. GER, greater epithelial ridge; IHC, inner hair cell; OHC, outer hair cell; SGN, spiral ganglion neuron.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11583137/'>39508782</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-fmed-07-617321-g001.jpgAnti-NEFH Antibody (Monoclonal, N52)Immunofluorescence identification of SCNs. (A) Cell nuclei are identified with DAPI. (B) SCNs are stained with the anti-NF200 monoclonal antibody. More than 90% of the cultured cells show positive expression of NF200 on the day 7 of ex vivo culture. The number of NF200-positive neurons are counted per area and expressed as percentage of total number of cells (Scale bar: 40 μm).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2020.617321/full'>33425964</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-nrr-14-1765-g003.jpgAnti-NEFH Antibody (Monoclonal, N52)GFAP, NF200 and MBP protein expression in nerve grafts 8 weeks after surgery. (A) Western blot assay for GFAP, NF200 and MBP in nerve grafts from over-TrkA, vector, TrkA-shRNA and control groups. (B) The bands were quantified by densitometry and normalized to β-actin. Data are expressed as the mean ± SEM ( n = 5 per group; one-way analysis of variance followed by Student-Newman-Keuls post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001, vs . vector and control groups. NF200: Neurofilament 200; GFAP: glial fibrillary acidic protein; MBP: myelin basic protein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6585565/&orig_host=www.ncbi.nlm.nih.gov'>31169194</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-41598_2018_34755_fig1_html.pngAnti-NEFH Antibody (Monoclonal, N52)Spared nerve injury-induced mechanical hypersensitivity and Nav1.7 activation in rat injured DRGs. ( A , B ) Ipsilateral ( A ) and contralateral ( B ) paw withdrawal threshold (PWL) before or after SNI or sham surgery. ** P < 0.01 vs. the baseline (BL). n = 6 rats/group. Two-way ANOVA followed by post hoc Tukey, F 1,5 = 22.63 in ( A ), F 1,5 = 1.809 in ( B ). ( C ) Levels of SCN9A mRNA in L4-6 DRGs at days shown after SNI or sham surgery in rats. n = 3 rats per group per time point. One-way ANOVA followed by post hoc Tukey, F 3,8 = 51.67 for SNI group, F 3,8 = 2.026 for sham group. ( D ) Levels of Nav1.7 protein in L4-6 DRGs at days shown after SNI surgery in rats. One-way ANOVA followed by post hoc Tukey, F 3,8 = 0.9785 in contralateral side (Cont), F 3,8 = 109.7 in ipsilateral side (Ipsi) * P < 0.05, ** P < 0.01, *** P < 0.001 vs. the zero day. n = 3 for each time point. ( E ) Co-localization of Nav1.7 with CGRP, IB4, and NF200 in rat L5 DRG, respectively. Scale bar: 100 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-34755-3'>30425258</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-2.jpgAnti-NEFH Antibody (Monoclonal, N52) Western blot analysis of NEFH using anti-NEFH antibody (MA1071). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NEFH antigen affinity purified monoclonal antibody (Catalog # MA1071)) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for NEFH at approximately 200KD. The expected band size for NEFH is at 112KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1071-nefh-primary-antibodies-ihc-testing-3.jpgAnti-NEFH Antibody (Monoclonal, N52) IHC analysis of NEFH using anti-NEFH antibody (MA1071).<br> NEFH was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-NEFH Antibody (MA1071) overnight at 4°C. Peroxidase Conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nitric-oxide-synthase-brain-1-181-nos1-antibody-monoclonal-ma1072-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Nitric Oxide Synthase, Brain(1-181) NOS1 Antibody (Monoclonal, NOS-B1)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p16ink4a-cdkn2-antibody-monoclonal-ma1074-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1074-1-s2.0-s0147651325012515-gr1.jpgAnti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)BPA exposure induces SW1353 cell senescence. (A) The effect of BPA on cell viability was examined by CCK-8 assay (n = 6). (B) Cellular senescence in SW1353 was detected by SA-β-gal staining (n = 6). (C, D) The gene and protein expression levels of cellular senescence markers p53, p21, p16 and BAG1 (n = 3). Data were presented as mean ± SD. * P < 0.05, ** P < 0.01 vs. control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S0147651325012515'>40848421</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1074-1-s2.0-s0147651325012515-gr3.jpgAnti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)BPA exposure induces cellular senescence by inhibiting SIRT1 in SW1353 cells. (A) SIRT1 protein expression in control and BPA-treated cells (n = 6). (B) Cell senescence in SW1353 cells was assayed by SA-β-gal staining (n = 6). (C) The gene expressions of p53, p21, p16 and BAG1 (n = 6). (D) The protein expressions of SIRT1, p53 and BAG1 in SW1353 cells (n = 5). Data were presented as mean ± SD. * P < 0.05 vs. control; # P < 0.05 vs. BPA group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S0147651325012515'>40848421</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1074-1-s2.0-s0147651325012515-gr6.jpgAnti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)BPA induces cell senescence and cell cycle arrest via repression of SIRT1 by miR-449a. (A) MiR-449a expression was determined by qPCR (n = 6). * P < 0.05 vs. control group, # P < 0.05 vs. miR-449a+SIRT1-WT. (B) Cell senescence in SW1353 cells was assayed by SA-β-gal staining (n = 6). (C, D) The protein expressions and quantification of SIRT1, p53 and BAG1 (n = 3). (E) The mRNA expression of SIRT1, p53, p21, p16 and BAG1 (n = 6). (F, H) The gene and protein expressions of cyclinD1, CDK4 and CDK6 (n = 3). (I) The gene expression of IL-1α, IL-1β, TGF-β and MMP13 (n = 6). Data were presented as mean ± SD. * P < 0.05 vs. NC; # P < 0.05 vs. BPA group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S0147651325012515'>40848421</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1074-cdkn2-primary-antibodies-ihc-testing-1.jpgAnti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)IHC analysis of CDKN2 using anti-CDKN2 antibody (MA1074).<br> CDKN2 was detected in a paraffin-embedded section of non-small cell lung cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with mouse anti-CDKN2 Antibody (MA1074) at 5 μg/ml overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p19ink4d-antibody-monoclonal-ma1075-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1075-p19ink4d-primary-antibodies-wb-testing-1.jpgAnti-p19INK4d Cdkn2d Antibody (Monoclonal, DCS-100) Western blot analysis of p19INK4d using anti-p19INK4d antibody (MA1075). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MOLT-4 whole cell lysates, <br> Lane 2: human HEL whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p19INK4d antigen affinity purified monoclonal antibody (Catalog # MA1075) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p19INK4d at approximately 18 kDa. The expected band size for p19INK4d is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p27kip1-antibody-monoclonal-ma1076-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk1-antibody-monoclonal-ma1077-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-CDK1 Antibody (Monoclonal, A17)Boster Kit Boxhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1077-41598_2016_article_bfsrep39047_fig1_html.jpgAnti-CDK1 Antibody (Monoclonal, A17)Real-time qPCR analysis of As-cdc20 and As-cdc23 expression levels at different developmental stages of A. sinica . Significant differences at different development stages ( P < 0.05) were analyzed by multi-factor analysis of variance (MANOVA) and indicated by lowercase letters (a, b and c). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep39047'>27991546</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1077-41598_2016_article_bfsrep39047_fig2_html.jpgAnti-CDK1 Antibody (Monoclonal, A17)The results of prokaryotic expression and antibody detection of As -CDC20 protein. (a) Expression analysis of the recombinant As -CDC20 protein. (b) Detection of the solubility of the recombinant As -CDC20 protein. (c) Western blotting analysis of the specific binding of the purified recombinant protein with antiserum antibodies. The full-length gels are presented in . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep39047'>27991546</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1077-41598_2016_article_bfsrep39047_fig3_html.jpgAnti-CDK1 Antibody (Monoclonal, A17)The results of prokaryotic expression and antibody detection of As -CDC23 protein. (a) Expression of the recombinant As -CDC23 protein. (b) Detection of the solubility of the recombinant As -CDC23 protein. (c) Purification of the recombinant As -CDC23 protein. (d) Western blotting analysis of the specific binding of the purified recombinant protein with antiserum antibodies. The full-length gels are presented in . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep39047'>27991546</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1077-41598_2016_article_bfsrep39047_fig4_html.jpgAnti-CDK1 Antibody (Monoclonal, A17)Western blotting analysis of As -CDC20, As -CDH1, As -CDC23, As -CDC14B, As -Securin, As -Cyclin B, As -CDK1 and As -Geminin. (a) Western blotting showing the expression of proteins at different developmental stages of Artemia sinica . (b) Values are expressed as arbitrary units of relative value. Significant differences ( P < 0.05) were analyzed by MANOVA and indicated by lowercase letters (a,b,c and d). The images are cropped, and the full-length blots are presented in . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep39047'>27991546</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1077-41598_2016_article_bfsrep39047_fig6_html.jpgAnti-CDK1 Antibody (Monoclonal, A17)Immunolocalization analysis of As -CDC23 at the embryo and sub-adult stages in A. sinica . A-A3: 0 h; B-B3: 15 h; C-C3: 3d; ( A–C ) represent anti-CDC23-label; A1-C1: single-labeling with DAPI; A2-C2 represent the image overlay of the samples dual-labeled with polyclonal anti-CDC23 and DAPI. A3-C3 represent control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep39047'>27991546</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1077-41598_2016_article_bfsrep39047_fig7_html.jpgAnti-CDK1 Antibody (Monoclonal, A17)The double-labeled immunofluorescence analysis of As -CDC20 and As -CDH1 in A. sinica . A–A3: gastrula stage; B-B3: embryonic stage; C-C3: metanauplius stage; ( A,B and C ) represent single-labeling with anti-CDC20; A1-C1: single-labeling with anti-CDH1; A2-C2 represent the image overlay of dual-labeled samples. A3–C3 represent the control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep39047'>27991546</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p53-antibody-monoclonal-ma1078-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1078-p53-primary-antibodies-wb-testing-1.jpgAnti-P53 Tp53 Monoclonal Antibody Western blot analysis of p53 using anti-p53 antibody (MA1078). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human Daudi whole cell lysates, <br> Lane 4: human A375 whole cell lysates, <br> Lane 5: human CCRF-CEM whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-p53 antigen affinity purified monoclonal antibody (Catalog # MA1078) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for p53 at approximately 53 kDa. The expected band size for p53 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1078-oncotarget-06-1020-g004.jpgAnti-P53 Tp53 Monoclonal AntibodyThe effect of CXCR4 on protein expression and cell cycle associated proteins in TNBC cells. (A) MDA-MB-231-NC and MDA-MB-231-shCXCR4 cells were treated with cisplatin (0, 10 μM) for 48 h, cellular protein was harvested, and Western blot analysis was performed to investigate the levels of mutant p53, Bax, Bcl-2 and caspase-3. The β-Actin was detected as a loading control. (B) MDA-MB-468-NC and MDA-MB-468-CXCR4 cells were treated with cisplatin (0, 10 μM) for 48 h, cellular protein was harvested, and Western blot analysis was performed to investigate the levels of mutant p53, Bax, Bcl-2 and caspase-3. The β-Actin was detected as a loading control. (C) Immunohistochemical staining of xenograft tumors for p53, Bax, Bcl-2 and caspase-3 (magnification = 40×).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4359214/&orig_host=www.ncbi.nlm.nih.gov'>25544759</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1078-2-IHC-anti-p53-antibody.jpgAnti-P53 Tp53 Monoclonal Antibody IHC analysis of p53 using anti-p53 antibody (MA1078). <br> p53 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-p53 Antibody (MA1078) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1078-3.jpgAnti-P53 Tp53 Monoclonal Antibody IF analysis of p53 using anti-p53 antibody (MA1078). <br> p53 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-p53 Antibody (MA1078) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pan-cadherin-antibody-monoclonal-ma1079-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-P-Cadherin-3 CDH3-Antibody (Monoclonal, CH-19)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-paxillin-antibody-monoclonal-ma1080-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1080-paxillin-primary-antibodies-wb-testing-1.jpgAnti-Paxillin Pxn Antibody (Monoclonal, PXC-10) Western blot analysis of Paxillin using anti-Paxillin antibody (MA1080). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human CACO-2 whole cell lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: rat RH35 whole cell lysates, <br> Lane 7: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Paxillin antigen affinity purified monoclonal antibody (Catalog # MA1080) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Paxillin at approximately 65 kDa. The expected band size for Paxillin is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pck-antibody-monoclonal-ma1081-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1081-1-IHC-anti-pck-antibody-monoclonal-c-11-pck-26-cy-90-ks-1a3-m20-a53-b-a2.jpgAnti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) IHC analysis of PCK using anti-PCK antibody (MA1081). <br> PCK was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 0.5 μg/ml mouse anti-PCK Antibody (MA1081) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1081-12906_2021_3213_fig1_html.pngAnti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)The characterization of PDLCs. Primary culture of PDLCs at the Day 7 (×100) ( a ); Morphology of PDLCs at Passage 1 (× 100) ( b ); Immunohistochemistry staining results of PDLCs, positive for vimentin (× 100) ( c ) and negative for cytokeratin (× 100) ( d ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12906-021-03213-5'>33485352</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1081-pck-primary-antibodies-wb-testing-2.jpgAnti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) Western blot analysis of PCK using anti-PCK antibody (MA1081). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human NCL-H460 whole cell lysates.<br> Lane 2: human Hela whole cell lysates.<br> Lane 3: human A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PCK antigen affinity purified monoclonal antibody (Catalog # MA1081) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCK at approximately 45-60 kDa. The expected band size for PCK is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pan-cytokeratin-antibody-monoclonal-ma1082-boster.html2026-03-24T05:03:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1082-ajtr0009-5708-f2.jpgAnti-Pan cytokeratin Antibody (Monoclonal, PCK-26)Identification of human periodontal ligament cells (PDLCs). Immunofluorescence staining showed that cultured PDLCs were positive for fibronectin and vimentin, and negative for pan cytokeratin, indicating their mesenchymal origin.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5752921/&orig_host=www.ncbi.nlm.nih.gov'>29312523</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1082-pan-cytokeratin-primary-antibodies-wb-testing-1.jpgAnti-Pan cytokeratin Antibody (Monoclonal, PCK-26) Western blot analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human NCL-H460 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: mouse skin tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Pan cytokeratin antigen affinity purified monoclonal antibody (Catalog # MA1082) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Pan cytokeratin at approximately 54 kDa. The expected band size for Pan cytokeratin is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1082-pan-cytokeratin-primary-antibodies-ihc-testing-2.jpgAnti-Pan cytokeratin Antibody (Monoclonal, PCK-26) IHC analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082). <br> Pan cytokeratin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml mouse anti-Pan cytokeratin Antibody (MA1082) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1082-pan-cytokeratin-primary-antibodies-if-testing-3.jpgAnti-Pan cytokeratin Antibody (Monoclonal, PCK-26) IF analysis of Pan cytokeratin using anti-Pan cytokeratin antibody (MA1082). <br> Pan cytokeratin was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL mouse anti-Pan cytokeratin Antibody (MA1082) overnight at 4°C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody at 1: 500 and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pcna-antibody-monoclonal-ma1083-boster.html2026-03-31T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-1_1.jpgAnti-PCNA Antibody (Monoclonal, PC 10) Western blot analysis of PCNA using anti-PCNA antibody (MA1083). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates&#44;<br> Lane 2: human MDA-MB-231 whole cell lysates&#44; <br> Lane 3: human Jurkat whole cell lysates&#44; <br> Lane 4: human HT1080 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PCNA antigen affinity purified monoclonal antibody (Catalog # MA1083) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCNA at approximately 35KD. The expected band size for PCNA is at 29KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-kvir_a_2384564_f0003_oc.jpgAnti-PCNA Antibody (Monoclonal, PC 10)PRRSV infection induced testicular growth and developmental disruption, apoptosis, and hormonal imbalances. (a) Immunostaining of porcine testis sections for SOX9 and PCNA expression. Scale bars = 50 μm. (b) Counts of SOX9+ and SOX9+ PCNA+ cells per cross-section of seminiferous cords/tubules in porcine testes are shown. (c) Immunostaining of porcine testis sections for β-catenin and ZO-1. Scale bars = 50 μm. (d) Detection of apoptosis in the testes by performing TUNEL assays. Scale bars = 50 μm. (e) ELISA analysis of serum T and AMH concentration. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant. (f) Analysis of dif-mRNAs revealed enrichment for GO terms associated with testicular growth and development, apoptosis, and hormone secretion. The top 20 GO pathways are shown. (g) Analysis of dif-mRNAs revealed enrichment for KEGG pathways associated with testicular growth and development, apoptosis, and hormone secretion. The top 15 KEGG pathways are shown. The rich factor indicates the ratio of dif-mRNAs enriched within a specific pathway. The sizes and colours of the solid circles represent the number of enriched dif-mRNAs and the significance of the enrichment, respectively. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.1080/21505594.2024.2384564'>39072452</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-gr6.jpgAnti-PCNA Antibody (Monoclonal, PC 10)KAZN overexpression inhibits cell proliferation and induces apoptosis.<br> (A and B) Western blot analysis (upper) and quantification (lower) of cleaved-caspase 3 (A) and PCNA (B) levels in A549 cells overexpressing KAZN.<br> (C and D) Western blot analysis (upper) and quantification (lower) of cleaved-caspase 3 (C) and PCNA (D) levels in NCI-H1299 cells overexpressing KAZN.<br> (E and F) EdU staining of A549 cells overexpressing KAZN and quantification of EdU-positive cells.<br> (G and H) EdU staining of NCI-H1299 cells overexpressing KAZN and quantification of EdU-positive cells. Scale bar: 200 μm.<br> Data were presented as mean ± SD from at least 3 independent experiments. p values were calculated using the unpaired Student’s t test. ∗p < 0.05.<br><br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/iscience/fulltext/S2589-0042(25)01846-2'>41126879</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1083-2-IHC-anti-pcna-antibody-monoclonal-pc-10.jpgAnti-PCNA Antibody (Monoclonal, PC 10) IHC analysis of PCNA using anti-PCNA antibody (MA1083).<br> PCNA was detected in paraffin-embedded section of human Rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1083-3-IF-anti-pcna-antibody-monoclonal-pc-10.jpgAnti-PCNA Antibody (Monoclonal, PC 10) IHC analysis of PCNA using anti-PCNA antibody (MA1083).<br> PCNA was detected in immunocytochemical section of human HELA Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-40168_2025_2128_fig6_html.jpgAnti-PCNA Antibody (Monoclonal, PC 10)5-MTP protects the gut barrier by promoting epithelial cell proliferation. A Survival rates of sham or CLP mice treated with 5-MTP or phosphate-buffered saline (PBS) ( n =5–10). B – D Serum TNF, PCT, and glucose concentration of CLP mice treated with 5-MTP or PBS ( n =6). E Serum FD-4 concentration in CLP mice treated with 5-MTP or PBS ( n =6). F Ileal H&E staining (left panel), AB-PAS staining (middle panel), and IF staining of TJ protein OCLN (right panel). The bar chart shows the quantification of ileal goblet cells ( n =6). G H&E staining, histopathological evaluation, and the wet/dry ratio of the lungs (scale bar= 50 μm, n =4–6). H PAS staining, histopathological evaluation of the kidneys, and the serum creatinine levels of the mice (scale bar= 50 μm, n =4–6). I Quantification of ileal 5-ethynyl-20-deoxyuridine (EdU) assay of 5-MTP or PBS-treated CLP mice (scale bar= 50 μm, n =6). J Ileal proliferating cell nuclear antigen (PCNA) protein level by Western blot ( n =6). K A schematic diagram illustrating the role of ACE2 in promoting intestinal epithelial cell repair in sepsis via the key microbial metabolite 5-MTP. Data are presented as the mean ± SD. ns=no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 by two-tailed Student’s t test or Mann-Whitney test. Intergroup survival differences were analyzed by the log-rank test. Abbreviations: 5-MTP, 5-methoxytryptophan; TNF, tumor necrosis factor; PCT, procalcitonin; FD-4, fluorescein isothiocyanate-dextran; Scr, serum creatinine<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12123736/'>40442816</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-fonc-15-1528004-g002.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Combination of PO and PD-1 blockade inhibited proliferation and induced apoptosis of tumor in vivo . (A) Paraffin sections of CT26 tumor tissues were analyzed by H&E staining (n = 3). (B) Expression and quantification of PCNA-positive staining in CT26 tumor tissues was examined by IHC using Image-Pro Plus 6.0 and in three random fields (n = 3). Scale bar, 50 μm. (C) TUNEL staining and the quantification of TUNEL-positive cells in CT26 tumor tissues (n = 3). Scale bar, 20 μm *p < 0.05, **p < 0.01, versus as indicated. ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11842225/'>39990679</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-41467_2025_56327_fig4_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)Less dynamic gel-like MSX1 phase separation impairs EPM proliferation. A , B HEPM ectopically expressing mEGFP-Vector, MSX-FL-mEGFP or its mutants, including R150S and R157S. A Flow cytometry assessing the cell cycle of HEPM. n = 3 biologically independent experiments. B RT-qPCR assessing the expression of proliferation marker genes, including CCND1, CCNA2, PCNA, and MKI67, in HEPM. n = 3 biologically independent experiments. C , D HEPM ectopically expressing mEGFP-Vector, MSX-FL-mEGFP or its mutants, including R150S, R157S, R150F, R157F, R150K and R157K. C Representative immunofluorescence staining images of PCNA in HEPM. Scale bars, 200 μm. D Quantification of PCNA-positive cells in HEPM. n = 4 biologically independent experiments. E – H HEPM transfected with siNC and siPRMT1. E Flow cytometry assessing the cell cycle of HEPM. F Quantification of flow cytometry in HEPM. n = 3 biologically independent experiments. G RT-qPCR assessing the expression of proliferation marker genes, including CCND1, CCNA2, PCNA, and MKI67. n = 3 biologically independent experiments. H Representative immunofluorescence staining images and quantification of PCNA in HEPM. Scale bars, 200 μm. n = 3 biologically independent experiments. I – L HEPM transfected with mEGFP-Vector, MSX-FL-mEGFP, MSX-FL-mEGFP+siNC, and MSX-FL-mEGFP+siPRMT1. I Flow cytometry assessing the cell cycle of HEPM. J Quantification of flow cytometry in HEPM. n = 3 biologically independent experiments. K RT-qPCR assessing the expression of proliferation marker genes, including CCND1, CCNA2, PCNA, and MKI67. n = 3 biologically independent experiments. L Representative immunofluorescence staining images and quantification of PCNA in HEPM. Scale bars, 200 μm. n = 4 biologically independent experiments. All data in this figure are represented as mean ± SD from at least three biologically independent experiments with similar results. Two-tailed Student’s t-test for ( F – H ), One-way ANOVA with Dunnett’s multiple comparisons test for ( A , B , D ), and Turkey’s multiple comparisons test for ( J – L ). All data are representative of at least three independent experiments. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-56327-6'>39843447</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12885_2024_12790_fig10_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)Knockdown of KRT23 inhibited tumor proliferation in vivo. ( A ) Western blotting analysis was conducted to detect the expression of KRT23 in BLCA tissues and adjacent normal tissues ( n = 6). ( B ) The tumor volumes were measured at the indicated time points. ( C ) The representative images of tumor. Tumor weight was measured after isolation from the mice. ( D ) IHC of PCNA and Ki67 in the tumors. *** p < 0.01, ** p < 0.01, * p < 0.05. Original blots are presented in supplementary Fig S6 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-024-12790-w'>39160525</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-jcav15p0176g011.jpgAnti-PCNA Antibody (Monoclonal, PC 10)knockdown of RIPK2 suppressed GC cell proliferation and apoptosis in vivo. (A-D) IHC was used to detect the expression of RIPK2 and PCNA in tumor section, and quantification. (E-F) TUNEL was performed to detect the apoptosis cells in tumor section, and the quantification of fluorescent intensity. * P < 0.05, ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10751663/&orig_host=www.ncbi.nlm.nih.gov'>38164277</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-41419_2022_5171_fig4_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)FGF-2 impedes the AAD-induced anti-EC effect via FGFR1-ERK-MYC signaling. A Cell growth of human ECs receiving the conditioned medium of scrambled- or FGF2 shRNA-transfected NPC tumor cells ( n = 5 samples per group). B Representative micrographs of PCNA + proliferative cells and DAPI signals in ECs treated with vehicle or recombinant human FGF-2. Scale bar = 50 μm. Quantification of PCNA + signals in and human ECs ( n = 8 random fields per group). C Vehicle- or VEGF-treated ECs were challenged with or without sunitinib or FGF-2. Phosphorylation of AKT and ERK in ECs was detected. β-actin marks the loading level in each lane ( n = 3 samples per group). D QPCR quantification of Fgfr1 , Fgfr2 , Fgfr3 , and Fgfr4 mRNA levels in ECs ( n = 3 samples per group). E Vehicle- or FGF-2-treated ECs were challenged with or without various FGFR inhibitors. Phosphorylation of ERK in ECs was detected. β-actin marks the loading level in each lane ( n = 3 samples per group). F Downstream of VEGF signaling transcription factors were selected and detected in vehicle- or FGF-2-treated ECs. Heatmap of qPCR array screened out Myc as the highest upregulated transcription factor. G Correlation of FGF2 and MYC transcriptomic expression levels of human NPCs (NPC, n = 113 samples). Data was extracted from dataset GSE102349. H QPCR quantification of Myc mRNA levels in isolated CD31 + ECs from scrambled- or FGF2 shRNA-transfected NPC tumor tissues ( n = 3 samples per group). I QPCR quantification of Myc mRNA levels in various groups of ECs ( n = 3 samples per group). J Vehicle- or VEGF-treated ECs were treated with or without AAD or FGF-2. MYC expression in ECs was detected. β-actin marks the loading level in each lane ( n = 3 samples per group). K QPCR quantification of Myc mRNA levels in vehicle- or FGF-2-treated ECs, with or without various inhibitors ( n = 3 samples per group). L Diagram of ETS-binding site prediction. M ChIP detection of ETS binding to the Myc gene promoter. Nonimmune IgG and Myc exon 2 regions served as controls ( n = 3 samples per group). N QPCR quantification of EC proliferative marker Kdr , Plxnd1 , Ptgs2 , Robo4 in scrambled- or Myc siRNA-transfected ECs administrated with vehicle or FGF-2 ( n = 3 samples per group). * P < 0.05; ** P < 0.01; *** P < 0.001. NS not significant. Data presented as mean ± SD. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-022-05171-3'>35985991</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-fphar-13-913408-g005.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Immunohistochemistry staining of wound tissues on days 7 and 14. (A) Representative images for CD31, PCNA, α-SMA, and CD68 staining (scale bar = 100 μm). (B–E) Quantification of CD31, PCNA, α-SMA, and CD68 protein expressions, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001 vs . control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs . CAH group as statistically significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9243309/&orig_host=www.ncbi.nlm.nih.gov'>35784748</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-fbioe-10-810244-g008.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Effect of asiaticoside on the transformation of fibroblasts into myofibroblasts, collagen deposition and fibroblast proliferation in vivo . Immunohistochemical staining of tissue around silicone rubber (SR) and carbon silicone rubber (C-SR) with α-SMA, vimentin, PCNA and COL-1A1 (100X).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9133697/&orig_host=www.ncbi.nlm.nih.gov'>35646845</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-fcell-10-820520-g002.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Increase proliferation of muscle cells in tibialis anterior in DMD mice. (A) Immunohistochemical staining of muscle cells was performed with the PCNA antibody. PCNA positive cells are shown as brown (red arrow). The PCNA positive cells were counted under microscope. Numbers of PCNA positive cells were demonstrated in the visual field Data were presented at means ± SEM ( n = 6) with independent sample t -test. ** p < 0.01. (B) Gene expression of tibialis anterior cells in WT and DMD mice was detected by RT-PCR. Data are expressed as the mean ± SEM ( n = 6). ** p < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.820520/full'>35372342</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-fcell-10-820520-g006.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Effects of GT3 on proliferation of muscle cells. (A) The tibialis anterior was extracted 48 h after GT3 treatment in 40-week-old WT and DMD mice. PCNA positive cells were stained with brown (red arrow, left panel). Numbers of PCNA positive cells were counted in each field. Data were presented at means ± SEM ( n = 6) with two-way ANOVA analysis. ** p < 0.01, *** p < 0.001. (B) Gene expression of tibialis anterior muscle cells was detected by RT-PCR after GT3 treatment. Data were presented at means ± SEM ( n = 6) with two-way ANOVA analysis. * p < 0.05, ** p < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2022.820520/full'>35372342</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-13578_2022_744_fig4_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)AIF blocks hypoxia-induced progression of PH in vitro and in vivo. A Hypoxia increased the viability of PASMCs after growth arrest for 24 h, and this effect was decreased by AIF (n = 4). B Pretreatment with an AIF overexpression plasmid blocked the effects of hypoxia on EdU incorporation in cells (n = 6). Scale bars: 50 μm. C Cell cycle analysis by flow cytometry indicated that hypoxia stimulated cell progression into G 2 /M + S phase, and this effect was inhibited by AIF overexpression (n = 3). D Effects of AIF on the expression of PCNA, Cyclin A and Cyclin D under hypoxia (n = 4–5). E Represents weight ratio of the right ventricular (RV)/left ventricular (LV) + Septum (n = 6); F Represents the right ventricular systolic pressure (RVSP) from mice (n = 5); G pulmonary artery velocity time integral (PAVTI), pulmonary artery acceleration time (PAAT) and left ventricular ejection fraction (LVEF) of the hypoxic mouse model infected with AAV5-NC and AAV5-AIF (n = 6). All data are presented as the means ± standard deviation. *p < 0.05; **p < 0.01; ***p < 0.001; Nor normoxia, Hyp hypoxia, NC negative control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-022-00744-3'>35090552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-13578_2022_744_fig5_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)AIF blocks hypoxia-induced pulmonary vascular remodeling in vivo. A Morphological analysis of the pulmonary artery was performed using HE staining and Masson staining, and the thickness of pulmonary vascular medium was measured by α-SMA staining (n = 5). B Increased proliferation of the pulmonary vascular cells was visualized by PCNA-positive staining per vascular area under hypoxia compared with exposure to normal conditions at the same time, these effects were reversed by the administration of AAV5-AIF (n = 3). C Homology analysis of the AIF gene among humans, mice and rats. Nor normoxia, Hyp hypoxia, NC negative control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-022-00744-3'>35090552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12935_2021_2374_fig2_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)SOX1 inhibits CCA cell proliferation in vitro and suppresses tumor growth in vivo. A TFK-1 and HUCCT-1 cells were transfected with NC-SOX1, LV-SOX1 and SOX1-KD for 72 h. SOX1 protein level was assessed by Western blotting. B Representative images of colony formation assay (left panel) and analysis of colony numbers (right panel). *p  < 0.05, **p  < 0.01, ****p  < 0.0001. C Cell proliferation was assessed by CCK-8 assay. ***p  < 0.001, ****p  < 0.0001. D Above panel: Xenograft tumors at day 18 after implantation of NC-SOX1 or LV-SOX1 cells into the right flank of nude mice. Below panel: comparison of tumor volumes between NC-SOX1 and LV-SOX1 xenograft mice. **p  < 0.01, ***p  < 0.001. E Protein of xenograft tumors was extract and PCNA, BCL2, SOX1 was assessed by Western blotting. F Flow cytometry detected apoptotic cells after cells were transfected with LV-SOX1 and NC-SOX1 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-021-02374-0'>34876142</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12935_2021_2374_fig4_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)Mir-155-5p inhibits SOX1 leading to activation of the Raf/MEK/ERK pathway. A Cells were transfected with lentiviral negative control vector (NC-SOX1) or lentiviral SOX1 (LV-SOX1) for 72 h. Protein expressions of SOX1, HES1, PROX1, p-AKT, p-JNK, and p-P38 were examined by Western blot. B Above panel: protein levels of ERK and p-ERK in HUCCT-1 and TFK-1 cells transfected with miR-negative control (miR-NC), miR-155-5p-mimic (miR-155-5p), and miR-155-5p-inhibitor (miR-155-5pI). Below panel: TFK-1 and HUCCT-1 cells was treated with different concentrations of miR-155-5pI. The protein level of ERK and p-ERK were detected by Western blot. C Protein levels of central members of MAPK/ERK signaling (RAF, p-RAF, MEK, p-MEK, ERK and p-ERK) and downstream of ERK (PCNA) in TFK-1 and HUCCT-1 cells were detected by Western blot. D TFK-1 and HUCCT-1 cells was transfected with miR-negative control (miR-NC) and miR-15-5p inhibitor (miR-155-5pI), then protein levels of central members of MAPK/ERK signaling (RAF, p-RAF, MEK, p-MEK, ERK and p-ERK) and PCNA were detected by Western blot. E TFK-1 and HUCCT-1 cells was transfected with lenvisual carrying SOX1-RNAi (SOX1-KD), then protein levels of central members of MAPK/ERK signaling (RAF, p-RAF, MEK, p-MEK, ERK and p-ERK) and PCNA were detected by Western blot. F Cells were infected with NC-SOX1, LV-SOX1, LV-SOX1  +  NC-ERK and LV-SOX1  +  LV-ERK, and then detect changes in ERK and PCNA protein levels by western blot. G Protein levels of central members of MAPK/ERK signaling (RAF, p-RAF, MEK, p-MEK, ERK and p-ERK) was detected in xenograft tumor samples which had been transfected with NC-SOX1 and LV-SOX1 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-021-02374-0'>34876142</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-13045_2021_1045_fig10_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)Immunohistochemical staining to evaluate the activity of carcinoma-associated fibroblast, the status of mediated epithelial-mesenchymal transition of cancer cells, as well as the proliferation and apoptosis of cancer cells. a H&E staining. b Anti-α-SMA staining. c Picrosirius red staining. d Anti-E-cadherin staining. e Anti-Vimentin staining. f Anti-Ki-67 staining. g Anti-PCNA staining. h Anti-cleaved-Caspase 3. For quantitative analysis, the integral optical density (IOD) of values the IHC stainings were calculated. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 denote the significant difference relative to YM101 treatment. Scale bars, 100 μm. α-TGF-β: anti-TGF-β, α-PD-L1: anti-PD-L1 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13045-021-01045-x'>33593403</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12957_2020_1896_fig1_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)Representative images of different LAIR-1 immunohistochemistry staining intensities in OS tissues. The proportion of positively stained cells for LAIR-1 was calculated by assessing the entire image. Based on the LAIR-1 staining intensities in OS tumor samples, the staining patterns were categorized as follows: weak (+), moderate (++), and intense (+++). Upper panel, original magnification × 200; lower panel, original magnification × 400. b Kaplan–Meier plot of survival rates of patients with tumors exhibiting high (blue line) or low (red line) LAIR-1 expression; data were obtained using the R2 platform. c Western blotting for determining LAIR-1 expression and PCNA proliferation marker levels in HOS cells following LV-NC or LV-LAIR-1 lentivirus infection or without treatment (blank). β-actin was used as a loading control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-020-01896-7'>32563267</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12951_2019_541_fig2_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)Anti-TNBC efficacy of Nano-DOX in comparison with DOX. a Effects of Nano-DOX and DOX on the viability of 4T1 cells in vitro assayed by the CCK-8 test. b Effects of Nano-DOX and DOX on the proliferation of 4T1 cells in vitro assayed by CFSE staining. c , d Apoptosis of 4T1 cells after 24-h treatment of Nano-DOX or DOX assayed by annexin V immunofluorescent staining and FACS. e , f Size and weight of orthotopic 4T1 tumor xenografts in mice at the end of 3-week treatment of Nano-DOX or DOX. g Immunohistochemical staining of Ki67, PCNA (markers of cancer cell proliferation), and caspase 3 (marker of cancer cell apoptosis) in mouse orthotopic 4T1 tumor xenografts at the end of 3-week treatment of Nano-DOX or DOX. (Duration of Nano-DOX or DOX treatment was 24 h for the in vitro cell experiments.) In FACS analysis, geometric means were used to quantify fluorescence intensity. Values were mean ± SD (n = 3 for in vitro experiments and n = 8 for in vivo experiments, *p < 0.05, **p < 0.01) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-019-0541-8'>31623629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-13287_2019_1368_fig4_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)hAECs facilitated endometrial recovery in the IUA mouse model. A IHC staining of vWF reflected the MVD of the endometrium. The microvessels, which were vWF-positive, are indicated by arrows in the figure. MVD was reduced in the IUA group and increased in the hAEC-treated group. B IHC staining showed that the expression of VEGF was higher in the hAEC-treated group than in the IUA group. C The expression of PCNA decreased in the IUA group and reached almost normal levels in the hAEC-treated group. a–c, scale bar = 100 μm; d–f, scale bar = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-019-1368-9'>31412924</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-13287_2019_1368_fig5_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)hAECs facilitated endometrial recovery in the IUA mouse model. A According to IHC staining, the number of ER-positive cells was higher in the hAEC-treated group than in the IUA group. B There was no difference in PR expression among these three groups. C VEGF expression was semi-quantified, and the number of positive cells per field was calculated. D MVD was valued by counting microvascular vessels, which were vWF-positive. E – G . PCNA, ER, and PR expression levels were semi-quantified by calculating the percentage of positive cells per field (* p < 0.05; ** p < 0.01; *** p < 0.001; NS, p ≥ 0.05). a–c, scale bar = 100 μm; d–f, scale bar = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-019-1368-9'>31412924</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-13287_2019_1368_fig8_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)hAECs promoted autophagy in hEnSCs in vitro. A The cell viability of H 2 O 2 -treated hEnSCs significantly decreased. B hEnSCs were cocultured with hAECs in a Transwell system. C After 2.5 h of H 2 O 2 treatment and another 24 h of culture, hEnSCs shrank severely, but hAEC coculture repaired the cell morphology of hEnSCs damaged by H 2 O 2 . D Western blot analysis showed that p62 expression increased significantly in H 2 O 2 -treated hEnSCs and decreased in hEnSCs cocultured with hAECs. The relative expression of LC3-II/LC3-I was decreased in H 2 O 2 -treated hEnSCs and increased in hEnSCs cocultured with hAECs. The expression level of ER was downregulated in H 2 O 2 -treated hEnSCs but upregulated in hEnSCs cocultured with hAECs. The expression of VEGF, PCNA, and PR did not change prominently. E The grayscale values of the western blots were evaluated. The ratios of LC3-II/LC3-I were standardized to those of the control group. The protein levels of p62, PCNA, VEGF, ER, and PR were normalized to that of β-tubulin ( n = 3; * p < 0.05; ** p < 0.01; *** p < 0.001; NS, p ≥ 0.05) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-019-1368-9'>31412924</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-13048_2019_541_fig5_html.pngAnti-PCNA Antibody (Monoclonal, PC 10)The effect of TEAS on irradiation-induced expression of Bax, Bcl-2 and PCNA proteins in ovary. a The protein expression levels of Bax, Bcl-2, PCNA were determined by western blot analysis. b – d Quantitative analysis of total proteins was represented using a bar graph. The data represent the mean ± SEM. * P < 0.05, ** P < 0.01 vs. control- group; # P < 0.05, ## P < 0.01 vs. IR 2D- group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-019-0541-1'>31324205</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-fphar-10-00128-g004.jpgAnti-PCNA Antibody (Monoclonal, PC 10)PNU-282987 treatment prevents and reveres pulmonary vascular remodeling. (A) Representative images of hematoxylin and eosin (HE), immunostaining of alpha-smooth muscle actin (α-SMA) and proliferating cell nuclear antigen (PCNA) from the lung in the sham, MCT, prevention and treatment groups. HE shows the thickness of the pulmonary artery. Brown staining with α-SMA indicates pulmonary artery smooth muscle, whereas brown-stained cells with PCNA represent proliferating pulmonary artery smooth muscle. The arrow indicates PCNA positive cells of the pulmonary artery. Occlusion (%), %vessels and PCNA positive cell (%) were calculated in a millimeter from 10 separate images of different fields. Original magnification: x400. (B) Graph showing the percentage of the median thickness of the arteriole. (C) Graph showing percentage of muscularization after α-SMA immunostaining. (D) Graph showing the percentage of PCNA positive cells. The data are summarized as means ± SD. ∗ P < 0.05 versus the sham group and # P < 0.05 versus the MCT group. Results shown are from one experiment (sham group, n = 15; MCT group, n = 8; MCT+protective group, n = 13; MCT+treatment group, n = 11).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00128/full'>30863307</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-wjg-21-5465-g004.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Pathway of phycocyanobilin-accelerated liver regeneration. A-E: Results of real-time quantitative PCR detection at 1, 2, 3 and 5 d after CCl 4 treament. A: The expression of HGF; B: The expression of TGF-α; C: The expression of TGF-β; D: The expression of TNF-α; E: The expression of IL-6; F: The western blotting result of PCNA, TNF-α, and cytochrome C in liver tissue. C1-C5 indicates the results of the control group from day 1 to day 5, D1-D5 indicates the results of the PCB group from day 1 to day 5. a P < 0.05, b P < 0.01 and d P < 0.001 vs control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4427667/&orig_host=www.ncbi.nlm.nih.gov'>25987768</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12885_2013_article_4302_fig7_html.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Tumorigenicity assay in nude mice. A : The growth rates of tumors formed from untransfected MFE-296 cells (MFE-296) and MFE-296 cells transfected with NC (MFE-296/NC) or shFOXA1 (MFE-296/shFOXA1). After injection, tumor volumes were calculated every seven days. B and C : Six weeks after injection of MFE-296, MFE-296-NC, and MFE-296-shFOXA1 cells, tumors were removed, and the tumor weights and volumes were determined. Arithmetic means and SD are shown. D : Staining with hematoxylin and eosin (H&E) or immunohistochemical staining for FOXA1, AR, Notch1, Hes1, Ki67, and PCNA in mouse tumor tissues (immunohistochemical staining, 200×). *p < 0.05 compared with the NC group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1471-2407-14-78'>24512546</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-medscimonit-18-10-br394-g005.jpgAnti-PCNA Antibody (Monoclonal, PC 10)AdSmad7-uPA treatment promoted hepatocyte proliferation and HGF maturation. ( A ) Liver sections were subjected to immunohistochemical staining using an anti-PCNA antibody to determine hepatocyte proliferation activity (×200). ( B ) Quantitation of the hepatic PCNA-labeling cells was shown. ( C ) Immunoblot analysis of mature HGF levels in livers using anti-HGF-β antibody that recognized only active HGF. ( D ) Quantitation of the mature HGF levels after normalization to β-actin. Data represent the mean ± standard deviation obtained from eight rats. # P<0.05 compared with AdGFP group; ¶ P<0.05 compared with AdSmad7 group; * P<0.01 compared with AdGFP group; § P<0.01 compared with AdSmad7 group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC3560566/&orig_host=www.ncbi.nlm.nih.gov'>23018346</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12868_2010_article_1915_fig2_html.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Localization of transplanted cells and in situ proliferation at the infarct border in experimental model . (A-C) are images of coronal cerebral slices showing the impact of occlusion 6 hours post-occlusion, with the infarct appearing as pale area marked with broken line. Hematoxylin and eosin (H.E.) stain illustrates loss of relatively large cells and infiltration of small cells at the border of the infarct (D). Paired cellular profiles are seen peripheral to the infarct border or the infarct penumbra (E). At low magnification, a large number of transplanted bone marrow cells pre-labeled by PKH26 (red fluorescence) are present in the infarct penumbra 7 days post lesion (F). The labeled cells are small and may occur in cluster (G). No fluorescent cells exist in the cerebral cortex of mice received vehicle infusion (H). In situ cell division reflected by immunoreactivity of proliferating cell nuclear antigen (PCNA) occurs predominantly at the infarct border (I) and penumbra (J), appearing as brown immunoreactive nuclei in hematoxylin counter-stained section. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-11-138'>20973978</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-12868_2010_article_1915_fig3_html.jpgAnti-PCNA Antibody (Monoclonal, PC 10)Identification of PKH26-labeled cells with proliferating cell nuclear antigen and doublecortin after transplantation . Colocalization of PKH26-labeled bone marrow cells with proliferating cell nuclear antigen (PCNA) (A-D) and doublecortin (DCX) (E-G) around the infarct penumbra 7 and 14 days following transplantation. PKH26 and PCNA double-labeled cells are small and occur in pair or small cluster (C, D). PKH26 and DCX double-labeled cells have round or oval somata with visible neuronal processes. PKH26 fluorescence appears weaker in these double-labeled cells relative to those seen at 7 days post cell transplantation. Scale bar (in B) = 200 μm for A, B and 50 μm for C-G. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://bmcneurosci.biomedcentral.com/articles/10.1186/1471-2202-11-138'>20973978</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-2-pcna-primary-antibodies-icc-testing-1.pngAnti-PCNA Antibody (Monoclonal, PC 10)IF analysis of PCNA using anti-PCNA antibody (MA1083). <br> PCNA was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:1000 mouse anti-PCNA Antibody (MA1083) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a laser confocol. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1083-2-pcna-primary-antibodies-ihc-review-1.pngAnti-PCNA Antibody (Monoclonal, PC 10) IHC analysis of PCNA using anti-PCNA antibody (MA1083).<br> PCNA was detected in paraffin-embedded section of HepG2 subcutaneous xenograft in nude mice tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 mouse anti-PCNA Antibody (MA1083) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkb-alpha-antibody-monoclonal-ma1085-boster.html2026-03-24T05:03:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1085-12868_2019_521_fig4_html.pngAnti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)Plantar incision induced a time-dependent increase of spinal pAkt expression in male mice, but not in female mice. The expression of pAkt and Akt was assayed at 0, 0.5, 2, 4, 8, 12, 24 h time-points after plantar incision in male mice ( a ) or female mice ( b ). The representative bands (top) for the expression of pAkt in the spinal cord at different time points after plantar incision and the quantitative data (bottom) for the expression of pAkt are shown. The fold change for the density of pAkt was normalized to total Akt for each sample, respectively. The fold change of pAkt in the 0-time point group was set at 1 for quantification. * P < 0.05, ** P < 0.01, compared with 0-time point group. (one-way ANOVA, followed by Bonferroni’s multiple comparison test; n = 4 mice in each group). Data are presented as mean ± SEM <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://bmcneurosci.biomedcentral.com/articles/10.1186/s12868-019-0521-9'>31366324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1085-12868_2019_521_fig5_html.jpgAnti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)Cellular localization of spinal-activated Akt induced plantar incision in male mice. Double immunofluorescence staining of pAkt (red) was performed with cell-specific markers: neuronal nuclei (NEUN, green) for neurons, ionized calcium-binding adapter molecule 1 (IBA1, green) for microglia, and glial fibrillary acidic protein (GFAP, green) for astrocytes at 2 h after plantar incision in male mice. Immunochemistry with pAkt antibody indicated increased pAkt immunoreactivity levels in the spinal dorsal horn ( B ). Double immunofluorescence staining showed that spinal pAkt was expressed in neurons and microglia (localization with NEUN or IBA1, respectively), but not expressed by astrocytes (no colocalization between pAkt and GFAP). Yellows: colocalization of pAkt with respective cell markers. n = 4 mice in each group. Scale bar = 200 μm ( A , B ); 50 μm ( C – K ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://bmcneurosci.biomedcentral.com/articles/10.1186/s12868-019-0521-9'>31366324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1085-12868_2019_521_fig6_html.pngAnti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)Post-surgical inhibition of PI3K attenuated spinal Akt activation induced by plantar incision in male mice. PI3K inhibitor wortmannin (0.4 μg) or LY294002 (5 μg) or DMSO was intrathecally injected at 45 min after plantar incision. The expression of spinal pAkt was assayed at the 2-h time-point after plantar incision. Post-surgical inhibition of PI3K with both wortmannin ( a ) and LY294002 ( b ) attenuated spinal Akt activation induced by plantar incision. The representative bands (top) and the quantitative data (bottom) for the expression of spinal pAkt after plantar incision. The fold change for the density of each pAkt was normalized to total Akt for each sample. The fold change of pAkt level in Sham-DMSO group was set at 1 for quantifications. ## P < 0.01 compared with Sham-DMSO group; * P < 0.05, compared with Incision-DMSO group (one-way ANOVA, followed by Bonferroni’s multiple comparison test; n = 4 mice in each group). Data are presented as mean ± SEM <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://bmcneurosci.biomedcentral.com/articles/10.1186/s12868-019-0521-9'>31366324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1085_fonc-15-1582040-g005.jpgAnti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)AMP treatment regulates the PI3K/AKT and MAPK signaling pathways. Lung cells (A549, NCI-H1299, and NCI-H520) with stable CHRM3 overexpression (A) or knockdown (B) were treated with or without 100 μg/mL AMPs for 48 h. The expression levels of PI3K/AKT pathway proteins and MAPK pathway proteins were detected using western blot.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12289679/'>40718823</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1085-akt1-primary-antibodies-wb-testing-1.jpgAnti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) Western blot analysis of PKB alpha using anti-PKB alpha antibody (MA1085). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat PC-12 whole cell lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PKB alpha antigen affinity purified monoclonal antibody (Catalog # MA1085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PKB alpha at approximately 60 kDa. The expected band size for PKB alpha is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pstair-antibody-monoclonal-ma1087-boster.html2026-03-24T05:03:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1087-pstair-primary-antibodies-wb-testing-1.jpgAnti-PSTAIR Antibody (Monoclonal, PSTAIR) Western blot analysis of PSTAIR using anti-PSTAIR antibody (MA1087). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates, <br> Lane 4: human THP-1 whole cell lysates, <br> Lane 5: rat C6 whole cell lysates, <br> Lane 6: rat PC-12 whole cell lysates, <br> Lane 7: mouse RAW264.7 whole cell lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PSTAIR antigen affinity purified monoclonal antibody (Catalog # MA1087) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PSTAIR at approximately 34 kDa. The expected band size for PSTAIR is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s-100-beta-subunit-antibody-monoclonal-ma1088-boster.html2026-03-24T05:03:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1088-1_2-IHC-anti-s-100-beta-subunit-antibody-monoclonal-sh-b1.jpgAnti-S-100(Beta-subunit) S100b Antibody (Monoclonal, SH-B1)Anti-S-100(beta-subunit) antibody (monoclonal)&#44; MA1088&#44; IHC(P)<br>IHC(P): Rat Brain Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-spectrin-alpha-and-beta-antibody-monoclonal-ma1090-boster.html2026-03-24T05:03:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Spectrin(Alpha And Beta) SPTB Antibody (Monoclonal, SB-SP1)Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-synaptophysin-antibody-monoclonal-ma1091-boster.html2026-03-24T05:03:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1091-1-WB-anti-synaptophysin-antibody-monoclonal-svp-38.jpgAnti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)Anti-Synaptophysin antibody (monoclonal)&#44; MA1091&#44; Western blotting<br>WB: Rat Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1091-4-IHC-anti-synaptophysin-antibody-monoclonal-svp-38.jpgAnti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)Anti-Synaptophysin antibody (monoclonal)&#44; MA1091&#44; IHC(P)<br>IHC(P): Human meningeoma Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1091-2-IHC-anti-synaptophysin-antibody-monoclonal-svp-38.jpgAnti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)Anti-Synaptophysin antibody (monoclonal)&#44; MA1091&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1091-3-IHC-anti-synaptophysin-antibody-monoclonal-svp-38.jpgAnti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)Anti-Synaptophysin antibody (monoclonal)&#44; MA1091&#44; IHC(P)<br>IHC(P): Human Glioma Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-talin-antibody-monoclonal-ma1092-boster.html2026-03-28T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1092-talin-primary-antibodies-wb-testing-1.jpgAnti-Talin TLN2 Antibody (Monoclonal, 8d4) Western blot analysis of Talin using anti-Talin antibody (MA1092). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates,<br> Lane 2: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Talin antigen affinity purified monoclonal antibody (Catalog # MA1092) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Talin at approximately 272 kDa. The expected band size for Talin is at 272 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tenascin-antibody-monoclonal-ma1094-boster.html2026-03-24T05:03:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1094-1-IHC-anti-tenascin-antibody-monoclonal-bc-24.jpgAnti-Tenascin TNN Antibody (Monoclonal, BC-24)Anti-Tenascin antibody (monoclonal)&#44; MA1094&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tropomyosin-36-39-kda-antibody-monoclonal-ma1095-boster.html2026-03-24T05:03:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1095-tropomyosin-primary-antibodies-wb-testing-1.jpgAnti-Tropomyosin(36/39 kDa) Tpm1 Antibody (Monoclonal, TM31) Western blot analysis of Tropomyosin(36/39kDa) using anti-Tropomyosin(36/39kDa) antibody (MA1095). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue lysates, <br> Lane 2: rat heart tissue lysates, <br> Lane 3: mouse skeletal muscle tissue lysates, <br> Lane 4: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tropomyosin(36/39kDa) antigen affinity purified monoclonal antibody (Catalog # MA1095) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tropomyosin(36/39kDa) at approximately 35-45 kDa. The expected band size for Tropomyosin(36/39kDa) is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-adaptin-antibody-monoclonal-ma1105-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1105-_-adaptin-primary-antibodies-wb-testing-1.jpgAnti-Alpha-Adaptin AP2A1 Antibody (Monoclonal, 100/2) Western blot analysis of α-Adaptin using anti-α-Adaptin antibody (MA1105). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates, <br> Lane 2: human U-87MG whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human 293T whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat C6 whole cell lysates, <br> Lane 7: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-α-Adaptin antigen affinity purified monoclonal antibody (Catalog # MA1105) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for α-Adaptin at approximately 108 kDa. The expected band size for α-Adaptin is at 108 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-hcg-antibody-monoclonal-ma1111-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ma1111-1-IHC-anti-beta-hcg-antibody-monoclonal-pc-2.jpgAnti-Beta-HCG CGB Antibody (Monoclonal, PC-2)Anti-beta-HCG antibody (monoclonal)&#44; MA1111&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gamma-tubulin-antibody-monoclonal-ma1114-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/a/ma1114-gamma-tubulin-primary-antibodies-wb-testing-1.jpgAnti-Gamma-Tubulin Antibody (Monoclonal, GTU-88) Western blot analysis of Gamma-Tubulin using anti-Gamma-Tubulin antibody (MA1114). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human COLO-320 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human U-87MG whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat C6 whole cell lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse HEPA1-6 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Gamma-Tubulin antigen affinity purified monoclonal antibody (Catalog # MA1114) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Gamma-Tubulin at approximately 55 kDa. The expected band size for Gamma-Tubulin is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fibronectin-antibody-monoclonal-ma1116-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Fibronectin Fn1 Monoclonal AntibodyBoster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-tnf-alpha-antibody-rp1000-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1000-1-WB-anti-tnf-alpha-antibody.jpgAnti-TNF alpha Antibody Picoband&reg;Figure. Western blot analysis of TNF alpha using anti-TNF alpha antibody (RP1000).<br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V(Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br><br> Lane: Recombinant Mouse TNFα Protein 0.5ng&#44;<br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # RP1000) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 17KD. The expected band size for TNF alpha is at 17KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1000-12865_2019_324_fig2_html.pngAnti-TNF alpha Antibody Picoband&reg;The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p < 0.05) or two ( p < 0.01) asterisks <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12865-019-0324-x'>31952480</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1000-12865_2019_324_fig3_html.pngAnti-TNF alpha Antibody Picoband&reg;miR32-5p up-regulated TNFɑ producing in BV2 cells. a The mRNA expression of TNFɑ in BV2 cells after LPS stimulated by qRT-PCR analyzed. b The protein expression of TNFɑ in BV2 cells after LPS stimulated by Dot-ELISA analyzed. c The gray value analysis of Dot-ELISA. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p < 0.05) or two ( p < 0.01) asterisks <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12865-019-0324-x'>31952480</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1000-12865_2019_324_fig5_html.pngAnti-TNF alpha Antibody Picoband&reg;The influence of PIKfyve for TNFɑ production in BV2 cells by qRT-PCR and Dot-ELISA analysis. a The expression changes of PIKfyve after treated with YM201636 by qRT-PCR analysis. b The expression changes of TNFɑ after treated with YM201636 by qRT-PCR analysis. c The expression changes of TNFɑ after treated with YM201636 by Dot-ELISA analysis. d The gray value analysis for Dot-ELISA. Bars represent mean ± S.D. (n ≥ 3). Significant difference marked with one ( p < 0.05) or two ( p < 0.01) asterisks <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12865-019-0324-x'>31952480</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rat-ifn-gamma-antibody-rp1001-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1001-1-WB-anti-ifn-gamma-antibody.jpgAnti-IFN gamma Antibody Picoband&reg;Figure. Western blot analysis of IFN gamma using anti-IFN gamma antibody (RP1001).<br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br><br> Lane : Recombinant Rat IFN gamma Protein 0.5ng <br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN gamma antigen affinity purified polyclonal antibody (Catalog # RP1001) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 22KD. The expected band size for IFN gamma is at 22KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-ifn-gamma-antibody-rp1002-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1002-1-WB-anti-ifn-gamma-antibody.jpgAnti-IFN gamma Antibody Picoband&reg;Figure . Western blot analysis of IFN gamma using anti-IFN gamma antibody (RP1002). <br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br><br> Lane: Recombinant Human IFN gamma Protein 0.5ng <br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN gamma antigen affinity purified polyclonal antibody (Catalog # RP1002) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 17KD. The expected band size for IFN gamma is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_221_fig1_html.jpgAnti-IFN gamma Antibody Picoband&reg;Percentages of NK (CD3 − CD56 + ) cells ( a – d , m ) in PBMC, NK cell group 2D receptor (NKG2D) + ( e – h , n ) and IFN-γ + ( i – l , o ) NK cells within total NK cells. HCs, healthy controls; IT, chronic HBV carriers; IA, CHB patients; IH, HBV-ACLF patients. Student-Newman-Keuls q test following one-way ANOVA were used for comparing percentages of NK cells, and Nemenyi test following Kruskal-Wallis H test were used for comparing percentages of NKG2D + and IFN-γ + NK cells between two groups. Compared with HCs group, ◆ ◆ ◆ P < 0.01; Compared with IT group, ★ ★ ★ P < 0.01, ★ ★ P < 0.05; Compared with IA group, ▲▲▲ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00221-9'>28273905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_221_fig2_html.jpgAnti-IFN gamma Antibody Picoband&reg;Intrahepatic expression of NKG2D mRNA ( a ) and IFN-γ mRNA ( b ). HCs, healthy controls (the relative expression were defined as 1.00); IT, chronic HBV carriers; IA, CHB patients; IH, HBV-ACLF. Nemenyi test following Kruskal-Wallis H test were used for comparing mRNA expressions of NKG2D and IFN-γ between two groups. Compared with HCs group, ◆ ◆ ◆ P < 0.01; Compared with IT group, ★ ★ ★ P < 0.01; Compared with IA group, ▲▲▲ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00221-9'>28273905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_221_fig3_html.jpgAnti-IFN gamma Antibody Picoband&reg;Representative graphs of intrahepatic IFN-γ + cells (A, 200×) and NKG2D + cells (B, 200×) expressions. ( a ) HCs, healthy controls, ( b ) IT, chronic HBV carriers, ( c ) IA, CHB patients, ( d ) IH, HBV-ACLF patients. ( e ) Collective analysis of results from all 4 groups. IFN-γ + cells were distributed mainly in the inflammatory sites and periportal areas that were infiltrated with lymphocytes. NKG2D + cells were mainly distributed in Disse’s space of hepatic lobule in HCs and chronic HBV carriers, and mainly in periportal areas in CHB and HBV-ACLF group. Nemenyi test following Kruskal-Wallis H test were used for comparing intrahepatic IFN-γ + and NKG2D + cells expressions between two groups. Compared with HCs group, ◆ ◆ ◆ P < 0.01; Compared with IT group, ★ ★ ★ P < 0.01; Compared with IA group, ▲▲▲ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00221-9'>28273905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_221_fig4_html.jpgAnti-IFN gamma Antibody Picoband&reg;Analysis of NKG2D and IFN-γ mRNA levels in co-cultured cells (NK + HepG2/HBV-HepG2) of Group A ( a , NK + HepG2) and Group B ( b , NK + HBV-HepG2). Nemenyi test following Kruskal-Wallis H test were used for comparing mRNA expressions of NKG2D and IFN-γ between two compared groups. Compared with Control group (NK + HepG2 or NK + HBV-HepG2), ◆ ◆ ◆ P < 0.01; Compared with Control + NKG2D mAb group, ★ ★ ★ P < 0.01; Compared with Control + IFN-α group, ▲▲▲ P < 0.01; Analysis of the levels of NKG2D and IFN-γ protein in different groups ( c ). The density of NKG2D and IFN-γ protein was the highest in group of NK + HBV-HepG2 + IFN-α, followed by group of NK + HBV-HepG2 + IFN-α + NKG2DmAb and group of NK + HBV-HepG2 + NKG2DmAb. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00221-9'>28273905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_221_fig5_html.jpgAnti-IFN gamma Antibody Picoband&reg;Serum Levels of IFN-γ, TNF-α, perforin and granzyme B in different clinical stages of chronic HBV-infected patients. HCs, healthy controls; IT, chronic HBV carriers; IA, CHB patients; IH, HBV-ACLF. Nemenyi test following Kruskal-Wallis H test were used for comparing IFN-γ, TNF-α, perforin and granzyme B levels between two compared groups. Compared with HCs group, ◆ ◆ ◆ P < 0.01, ◆ ◆ P < 0.05; Compared with IT group, ★ ★ ★ P < 0.01, ★ ★ P < 0.05; Compared with IA group, ▲▲▲ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00221-9'>28273905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_221_fig6_html.jpgAnti-IFN gamma Antibody Picoband&reg;Levels of TNF-α ( a ) and IFN-γ ( b ) in the supernatants with or without co-cultured NK cells. Student-Newman-Keuls q test following one-way ANOVA were used for comparing IFN-γ, TNF-α, perforin and granzyme B levels between two compared groups. Compare with HepG2 cells group, ◆ ◆ ◆ P < 0.01; Compare with HBV-HepG2 cells group, ◇ ◇ ◇ P < 0.01; Compare with NK cells group, ★ ★ ★ P < 0.01. Levels of perforin ( c ) and granzyme B ( d ) in the supernatants in the presence IFN-α with or without NKG2D blocking. Compare with control group, ▲▲▲ P < 0.01; Compare with + IFN-α group, ■■■ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00221-9'>28273905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_article_bfsrep40404_fig1_html.jpgAnti-IFN gamma Antibody Picoband&reg;Serum levels of IP-10, IFN-γ, IL-4, and TGF-β1 as well as the IFN-γ/IL-4 ratio in chronic hepatitis B patients with or without fibrosis. The levels of serum IP-10, IFN-γ, IL-4, and TGF-β1 in CHB patients with or without liver fibrosis were determined by ELISA, and the IFN-γ/IL-4 ratio was calculated. ♦♦♦ Differs from controls (the F0 group), P < 0.05; ★ ★ ★ differs from mild or minimal fibrosis (the F1–2 group), P < 0.05; ▴ ▴ ▴ differs from moderate fibrosis (the F3–4 group), P < 0.05. ( a ) IP-10; ( b ) IFN-γ; ( c ) IL-4; ( d ) TGF-β1; ( e ) the IFN-γ/IL-4 ratio. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_article_bfsrep40404_fig2_html.jpgAnti-IFN gamma Antibody Picoband&reg;Statistical analysis of the correlation between the serum IP-10 level or the IFN-γ/IL-4 ratio with liver fibrosis among chronic hepatitis B patients. Spearman’s correlation analysis of the association between ( a ) IP-10; ( b ) IFN-γ; ( c ) the IFN-γ/IL-4 ratio and TGF-β1. Spearman’s correlation analysis of the association between serum ( d ) IFN-γ; ( e ) IL-4; ( f ) the IFN-γ/IL-4 ratio and IP-10. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_article_bfsrep40404_fig3_html.jpgAnti-IFN gamma Antibody Picoband&reg;ROC curve analysis for evaluating the sensitivity and specificity of the IP-10 level, IFN-γ/IL-4 ratio, or their combination to predict significant fibrosis among CHB patients. ( a ) ROC curve analysis for serum IP-10 (with the cut-of f value of 300 pg/mL), the serum IFN-γ/IL-4 ratio (with the cut off value of 1.8), and the combination of IP-10 and the IFN-γ/IL-4 ratio; ( b ) Specificity and sensitivity for IP-10, the IFN-γ/IL-4 ratio, and their combination to predict significant liver fibrosis among patients with CHB. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_article_bfsrep40404_fig4_html.jpgAnti-IFN gamma Antibody Picoband&reg;Intrahepatic mRNA levels of IP-10, IFN-γ, and IL-4 in chronic hepatitis B patients with or without fibrosis. Real-time qRT-PCR was conducted to quantify the mRNA levels of intrahepatic IP-10, IFN-γ, and IL-4 in the CHB patients without or with fibrosis as described in the Materials and Methods section. The relative mRNA levels of intrahepatic IP-10, IFN-γ, and IL-4 were calculated by comparative Ct analysis after normalization for the quantity of GAPDH in the same samples and were represented as 2 - △ △ Ct values for controls (the F0 group), which were set equal 1. ♦♦♦ Differs from controls (the F0 group), P < 0.05; ★ ★ ★ differs from mild or minimal fibrosis (the F1–2 group), P < 0.05; ▴ ▴ ▴ differs from moderate fibrosis (the F3–4 group), P < 0.05. (a) IP-10; (b) IFN-γ; (c) IL-4. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-41598_2017_article_bfsrep40404_fig5_html.jpgAnti-IFN gamma Antibody Picoband&reg;Intrahepatic protein expression of IP-10, IFN-γ, IL-4, TGF-β1, and α-SMA as well as the IFN-γ/IL-4 ratio in chronic hepatitis B patients with or without fibrosis. The protein expression of intrahepatic (a, b, c, and d) IP-10, (e, f, g, and h) IFN-γ, and (i, g, k, and l) IL-4. In addition, the protein levels of intrahepatic IP-10, IFN-γ, IL-4, TGF-β1, and α-SMA were quantified based on the value of integrated optical density (IOD) and represented as histograms, from which the IFN-γ/IL-4 ratio was calculated. ♦♦♦ Differs from controls (the F0 group), P < 0.05; ★ ★ ★ differs from mild or minimal fibrosis (the F1–2 group), P < 0.05; ▴ ▴ ▴ differs from moderate fibrosis (the F3–4 group), P < 0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1002-ifn-gamma-primary-antibodies-ihc-testing-2.jpgAnti-IFN gamma Antibody Picoband&reg; IHC analysis of IFN gamma using anti-IFN gamma antibody (RP1002). <br> IFN gamma was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-IFN gamma Antibody (RP1002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-bcl-2-antibody-rp1003-boster.html2026-03-24T05:03:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1003-1-WB-anti-human-bcl-2-antibody.jpgAnti-Bcl2 alpha Antibody Picoband&reg;Figure. Western blot analysis of BCL-2 using anti-BCL-2 antibody (RP1003). <br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br><br> Lane : Recombinant Human Bcl-2 Protein 0.5ng&#44;<br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL-2 antigen affinity purified polyclonal antibody (Catalog # RP1003) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL-2 at approximately 26KD. The expected band size for BCL-2 is at 26KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-epo-antibody-rp1004-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1004-1-WB-anti-epo-erythropoietin-antibody.jpgAnti-EPO/Erythropoietin Antibody Picoband&reg;Anti-human EPO antibody&#44; RP1004&#44; Western blotting<br>Lane 1: Recombinant human EPO Protein 10ng <br>Lane 2: Recombinant human EPO Protein 5ng <br>Lane 3: Recombinant human EPO Protein 2https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1004-2-IHC-anti-epo-erythropoietin-antibody.jpgAnti-EPO/Erythropoietin Antibody Picoband&reg;Anti-human EPO antibody&#44; RP1004&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-fgf1-antibody-rp1005-boster.html2026-03-25T05:21:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1005-2-IHC-anti-fgf1-fgf-acidic-antibody.jpgAnti-Fibroblast growth factor 1 FGF1 Antibody Picoband&reg;Anti-human FGF1 antibody&#44; RP1005&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1005-1_1.jpgAnti-Fibroblast growth factor 1 FGF1 Antibody Picoband&reg;Anti-human FGF1 antibody&#44; RP1005&#44; Western blotting<br>Lane 1: Recombinant Human FGF1 Protein 10ng<br>Lane 2: Recombinant Human FGF1 Protein 5ng<br>Lane 3: Recombinant Human FGF1 Protein 2 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-fgf2-antibody-rp1006-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1006-fgf2-primary-antibodies-ihc-testing-1_1.jpgAnti-Fibroblast growth factor 2 FGF2 Antibody Picoband&reg;IHC analysis of FGF2 using anti-FGF2 antibody (RP1006). <br> FGF2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FGF2 Antibody (RP1006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1006-fgf2-primary-antibodies-wb-testing-1.jpgAnti-Fibroblast growth factor 2 FGF2 Antibody Picoband&reg; Western blot analysis of FGF2 using anti-FGF2 antibody (RP1006). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SKOV3 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates, <br> Lane 4: human u87 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # RP1006) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17-21 kDa. The expected band size for FGF2 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1006-fgf2-primary-antibodies-if-testing-2.jpgAnti-Fibroblast growth factor 2 FGF2 Antibody Picoband&reg; IF analysis of FGF2 using anti-FGF2 antibody (RP1006). <br> FGF2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FGF2 Antibody (RP1006) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1006-fgf2-primary-antibodies-fcm-testing-3.jpgAnti-Fibroblast growth factor 2 FGF2 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-FGF2 antibody (RP1006). <br> Overlay histogram showing SiHa cells stained with A04887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FGF2 Antibody (RP1006, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rat-growth-hormone-antibody-rp1007-boster.html2026-03-25T05:21:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1007-gh1-primary-antibodies-wb-testing-1.jpgAnti-Growth Hormone/GH1 Antibody Picoband&reg; Western blot analysis of GH1 using anti-GH1 antibody (RP1007). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 5 ng of sample under reducing conditions. <br> Lane 1: rat GH1 recombinant protein.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GH1 antigen affinity purified polyclonal antibody (Catalog # RP1007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 22 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1007-gh1-primary-antibodies-ihc-testing-2.jpgAnti-Growth Hormone/GH1 Antibody Picoband&reg; IHC analysis of GH1 using antiGH1 antibody (RP1007). <br> GH1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GH1 Antibody (RP1007) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il-3-antibody-rp1008-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1008-1-WB-anti-il-3-antibody.jpgAnti-Interleukin-3 IL3 Antibody Picoband&reg;Figure. Western blot analysis of IL-3 using anti-IL-3 antibody (RP1008). <br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br><br> Lane : Recombinant Human IL-3 Protein 0.5ng<br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-3 antigen affinity purified polyclonal antibody (Catalog # RP1008) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-3 at approximately 15KD. The expected band size for IL-3 is at 15KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il-4-antibody-rp1009-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1009-1-WB-anti-il-4-antibody.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Figure. Western blot analysis of IL-4 using anti-IL-4 antibody (RP1009). <br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br><br> Lane: Recombinant Human IL-4 Protein 0.5ng&#44;<br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-4 antigen affinity purified polyclonal antibody (Catalog # RP1009) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-4 at approximately 14KD. The expected band size for IL-4 is at 14KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1009-12951_2021_1236_fig4_html.pngAnti-Interleukin-4 IL4 Antibody Picoband&reg;Protective effect of M2 macrophages induced by hUCMSCs-EVs on OA chondrocytes in vitro. A IL-1β-induced OA chondrocytes were co-cultured with the supernatant of M2 macrophages (M2S) induced by hUCMSCs-EVs, or platelet-rich plasma (PRP) for 48 h, relative mRNA expression of the key genes TNF-α, MMP13, SOX9, and ACAN was measured by quantitative RT-PCR analysis; the experiment was performed triplicate; *p < 0.05, **p < 0.01, ***p < 0.001. B Western blot was performed to evaluate the expression of TNF-α, MMP13, and IL-4 proteins in PBS, M2S, or PRP-treated OA chondrocytes; GAPDH was employed as the loading control; *p < 0.05, **p < 0.01, ***p < 0.001. C The influence of M2S or PRP on the viability of chondrocytes was detected by the cell live/death experiment; green represents live cells while red represents dead cells; Scale bar: 1 mm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-021-01236-1'>35057811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1009-41598_2017_article_bfsrep40404_fig1_html.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Serum levels of IP-10, IFN-γ, IL-4, and TGF-β1 as well as the IFN-γ/IL-4 ratio in chronic hepatitis B patients with or without fibrosis. The levels of serum IP-10, IFN-γ, IL-4, and TGF-β1 in CHB patients with or without liver fibrosis were determined by ELISA, and the IFN-γ/IL-4 ratio was calculated. ♦♦♦ Differs from controls (the F0 group), P < 0.05; ★ ★ ★ differs from mild or minimal fibrosis (the F1–2 group), P < 0.05; ▴ ▴ ▴ differs from moderate fibrosis (the F3–4 group), P < 0.05. ( a ) IP-10; ( b ) IFN-γ; ( c ) IL-4; ( d ) TGF-β1; ( e ) the IFN-γ/IL-4 ratio. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1009-41598_2017_article_bfsrep40404_fig2_html.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Statistical analysis of the correlation between the serum IP-10 level or the IFN-γ/IL-4 ratio with liver fibrosis among chronic hepatitis B patients. Spearman’s correlation analysis of the association between ( a ) IP-10; ( b ) IFN-γ; ( c ) the IFN-γ/IL-4 ratio and TGF-β1. Spearman’s correlation analysis of the association between serum ( d ) IFN-γ; ( e ) IL-4; ( f ) the IFN-γ/IL-4 ratio and IP-10. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1009-41598_2017_article_bfsrep40404_fig3_html.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;ROC curve analysis for evaluating the sensitivity and specificity of the IP-10 level, IFN-γ/IL-4 ratio, or their combination to predict significant fibrosis among CHB patients. ( a ) ROC curve analysis for serum IP-10 (with the cut-of f value of 300 pg/mL), the serum IFN-γ/IL-4 ratio (with the cut off value of 1.8), and the combination of IP-10 and the IFN-γ/IL-4 ratio; ( b ) Specificity and sensitivity for IP-10, the IFN-γ/IL-4 ratio, and their combination to predict significant liver fibrosis among patients with CHB. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1009-41598_2017_article_bfsrep40404_fig4_html.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Intrahepatic mRNA levels of IP-10, IFN-γ, and IL-4 in chronic hepatitis B patients with or without fibrosis. Real-time qRT-PCR was conducted to quantify the mRNA levels of intrahepatic IP-10, IFN-γ, and IL-4 in the CHB patients without or with fibrosis as described in the Materials and Methods section. The relative mRNA levels of intrahepatic IP-10, IFN-γ, and IL-4 were calculated by comparative Ct analysis after normalization for the quantity of GAPDH in the same samples and were represented as 2 - △ △ Ct values for controls (the F0 group), which were set equal 1. ♦♦♦ Differs from controls (the F0 group), P < 0.05; ★ ★ ★ differs from mild or minimal fibrosis (the F1–2 group), P < 0.05; ▴ ▴ ▴ differs from moderate fibrosis (the F3–4 group), P < 0.05. (a) IP-10; (b) IFN-γ; (c) IL-4. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1009-41598_2017_article_bfsrep40404_fig5_html.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Intrahepatic protein expression of IP-10, IFN-γ, IL-4, TGF-β1, and α-SMA as well as the IFN-γ/IL-4 ratio in chronic hepatitis B patients with or without fibrosis. The protein expression of intrahepatic (a, b, c, and d) IP-10, (e, f, g, and h) IFN-γ, and (i, g, k, and l) IL-4. In addition, the protein levels of intrahepatic IP-10, IFN-γ, IL-4, TGF-β1, and α-SMA were quantified based on the value of integrated optical density (IOD) and represented as histograms, from which the IFN-γ/IL-4 ratio was calculated. ♦♦♦ Differs from controls (the F0 group), P < 0.05; ★ ★ ★ differs from mild or minimal fibrosis (the F1–2 group), P < 0.05; ▴ ▴ ▴ differs from moderate fibrosis (the F3–4 group), P < 0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep40404'>28067328</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-scf-antibody-rp1010-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1010-1-WB-anti-scf-antibody.jpgAnti-SCF/KITLG Antibody Picoband&reg;Figure. Western blot analysis of SCF using anti-SCF antibody (RP1010). <br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br><br> Lane : Recombinant Human SCF Protein 0.5ng&#44;<br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCF antigen affinity purified polyclonal antibody (Catalog # RP1010) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCF at approximately 18KD. The expected band size for SCF is at 18KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1010-scf-primary-antibodies-ihc-testing-1.jpgAnti-SCF/KITLG Antibody Picoband&reg;IHC analysis of SCF using anti-SCF antibody (RP1010). <br>SCF was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCF Antibody (RP1010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-ddt-antibody-rp1011-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1011-ddt-primary-antibodies-wb-testing-1.jpgAnti-D-dopachrome decarboxylase DDT Antibody Picoband&reg; Western blot analysis of DDT using anti-DDT antibody (RP1011, Left) and anti-DDT antibody (PA1449, Right). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, <br> Lane 2: rat liver tissue lysates,<br> Lane 3: mouse liver tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # RP1011) and rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # PA1449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 13-15 kDa. The expected band size for DDT is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1011-2.jpgAnti-D-dopachrome decarboxylase DDT Antibody Picoband&reg; IHC analysis of DDT using anti-DDT antibody (RP1011). <br> DDT was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDT Antibody (RP1011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il-6-antibody-rp1012-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1012-il-6-primary-antibodies-wb-testing-1.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg; Western blot analysis of IL-6 using anti-IL-6 antibody (RP1012). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human IL6 protein 10 ng,<br> Lane 2: recombinant human IL6 protein 5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1012-nrr-12-1632-g003.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Effect of glycogen synthase kinase 3 beta (GSK-3β) on Bax, Bcl-2, interleukin (IL)-6, IL-8, and IL-10 immunoreactivity in the brain of diabetic rats with myocardial ischemia/reperfusion injury (%). (A–E) Representative images (original magnification, 400×) and quantification of Bax, Bcl-2, IL-6, IL-8, and IL-10 immunoreactivities. Arrows show Bax-, Bcl-2-, IL-6-, IL-8-, and IL-10-immunoreactive cells. ** P < 0.01, vs . NS group; ## P < 0.01, vs . NIR group; $$ P < 0.01, vs . NIPost group; && P < 0.01, vs . NIPostI group; §§ P < 0.01, vs . DS group; †† P < 0.01, vs . DIR group; ££ P < 0.01, vs . DIPost group (mean ± SD, n = 4, one-way analysis of variance and the Student-Newman-Keuls test). The results for the NIPostI group resembled those for the NIR group. Histopathological changes were more severe in diabetic vs . non-diabetic rats. Only the NS and DS groups received a thoracotomy. Myocardial ischemia reperfusion was conducted in the NIR and DIR groups by blocking the left anterior descending coronary artery. The NIPost and DIPost groups were subjected to three cycles of 10-second reperfusion followed by a 10-second ischemia treatment immediately at the onset of reperfusion. The NIPostI and DIPostI groups were intraperitoneally injected with GSK-3β inhibitor 10 minutes before receiving MIRI, and received three cycles of 10-second reperfusion followed by a 10-second ischemia treatment immediately at the onset of reperfusion. NS group: Normal sham group; NIR group: normal myocardial ischemia reperfusion group; NIPost group: normal ischemic post-conditioning group; NIPostI group: normal ischemic post-conditioning + GSK-3β inhibitor group; DS group: diabetic sham group; DIR group: diabetic myocardial ischemia reperfusion group; DIPost group: diabetic ischemic post-conditioning group; DIPostI group: diabetic ischemic post-conditioning + GSK-3β inhibitor group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5696844/&orig_host=www.ncbi.nlm.nih.gov'>29171428</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1012-13046_2009_article_237_fig2_html.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Real-time PCR analysis of upregulated or downregulated gene expression in response to HIF-1alpha (A) Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time PCR . In three pairwise comparisons, the upregulation-folds of IGFBP5, IRS4, TNFAIP6, SOCS1, IL-6, VEGF-A mRNA expression were calculated. The mean and standard error are shown (p < 0.05). (B) Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time PCR. In three pairwise comparisons, the downregulation-folds of IGFBP3, ZNF569, SOCS2, SIRPa and XRCC4 mRNA were calculated. The mean and standard error are shown (p < 0.05). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-28-150'>20003295</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1012-13046_2009_article_237_fig3_html.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Western blot analysis of regulation of protein expression by HIF-1alpha in NCI-H446 cells . According to different treatments, all the cells were divided into four groups: control group (the cells cultured under normoxic conditions of 20% O2), Ad5-HIF-1alpha transfection group, hypoxia group (the cells cultured under normoxic conditions of 1% O2) and Ad5-siHIF-1alpha transfection group (after transfection, the cells were cultured under normoxic conditions of 1% O2). (A) Western blot analysis for IGFBP5 protein expressed by the cells of four groups. (B) Western blot analysis for SOCS1 protein expressed by the cells of four groups. (C) Densitometric analysis of the IGFBP5 and SOCS1 bands compared to the corresponding β-actin bands (*p < 0.05 expression of IGFBP5 or SOCS1 protein in Ad5-HIF-1alpha group vs. control group; ** p < 0.05 expression of IGFBP5 or SOCS1 protein in hypoxia group vs. control group; *** p < 0.05 expression of IGFBP5 or SOCS1 protein in Ad5-siHIF-1alpha group vs. control group). (D) Western blot analysis for IL-6 protein expressed by the cells of four groups. (E) Western blot analysis for STAT3 protein expressed by the cells of four groups. (F) Densitometric analysis of the IL-6 and STAT3 bands compared to the corresponding β-actin bands (*p < 0.05 expression of IL-6 or STAT3 protein in Ad5-HIF-1alpha group vs. Ad5-siHIF-1alpha group group.) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-28-150'>20003295</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1012-jingwen_zhang.pngAnti-Interleukin-6 IL6 Antibody Picoband&reg; Western blot analysis of IL-6 using anti-IL-6 antibody (RP1012). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1-4: human HK-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # RP1012) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il7-antibody-rp1013-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1013-1-WB-anti-il-7-antibody.jpgAnti-Interleukin-7 IL7 Antibody Picoband&reg;Anti-human IL7 antibody&#44; RP1013&#44; Western blotting<br>Lane 1: Recombinant Human IL-7 Protein 10ng <br>Lane 2: Recombinant Human IL-7 Protein 5ng<br>Lane 3: Recombinant Human IL-7 Protein 2https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1013-2.jpgAnti-Interleukin-7 IL7 Antibody Picoband&reg; Western blot analysis of IL-7 using anti-IL-7 antibody (RP1013). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates&#44; <br> Lane 2: human Hela whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-7 antigen affinity purified polyclonal antibody (Catalog # RP1013) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-7 at approximately 20KD. The expected band size for IL-7 is at 20KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il-10-antibody-rp1014-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1014-il10-primary-antibodies-wb-testing-1.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg;Western blot analysis of IL10 using anti-IL10 antibody (RP1014). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human IL10 protein 10 ng,<br> Lane 2: recombinant human IL10 protein 5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (RP1014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1014-il10-primary-antibodies-ihc-testing-1.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg;IHC analysis of IL10 using anti-IL10 antibody (RP1014). <br> IL10 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1014-il10-primary-antibodies-ihc-testing-2_1.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg;IHC analysis of IL10 using anti-IL10 antibody (RP1014). <br> IL10 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-il-10-antibody-rp1015-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1015-1-WB-anti-il-10-antibody.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg;Anti-mouse IL-10 antibody&#44; RP1015&#44; Western blotting<br>Lane 1: Recombinant Mouse IL-10 Protein 10ng<br>Lane 2: Recombinant Mouse IL-10 Protein 5ng<br>Lane 3: Recombinant Mouse IL-10 Protein 2https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1015-fmicb-11-622354-g002.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg;The infiltration of MPO + neutrophils, and the cellular distribution and relative expression level detection of the TNF and IL-10 in the small intestinal and colonic mucosa at 7 days after the termination of DSS administration. (A) The MPO immunohistochemistry staining of the small intestinal mucosa: (A1) the normal group: few neutrophils were observed in the small intestinal mucosa; (A2) the DSS group: a number of accumulative MPO + neutrophils (brown) infiltrated into the mucosa epithelium; (A3) the DSS + B. subtilis- fermented milk group: only limited neutrophil infiltration could be observed in the small intestinal mucosa. (B) The MPO immunohistochemistry staining of the colonic mucosa: (B1) the normal group: few neutrophils were observed in the colonic mucosa; (B2) the DSS group: colonic epithelium and the glands disappeared, and the ulcer was locally replaced by scars and a number of accumulative MPO + neutrophils (brown) were observed in the scars; (B3) the DSS + B. subtilis -fermented milk group: only limited MPO + neutrophils observed in the colonic mucosa. (C) The TNF immunohistochemistry staining of the small intestinal mucosa: (C1) the normal group: the epithelium was integrated with faint yellow staining, suggesting low expression of TNF; (C2) the DSS group: the villus structure is not integrated, and the epithelial cells showed black brown, suggesting overexpression of TNF; (C3) the DSS + B. subtilis -fermented milk group: the villus and the glands were almost integrated, and the staining of epithelial cells was similar to that of the normal group (C1) , suggesting low expression of TNF. (D) The TNF immunohistochemistry staining of the colonic mucosa: (D1) the normal colonic mucosa: the epithelium was integrated with low TNF expression (faint yellow); ( D2 ) the DSS group: the epithelium structure and the glands were destroyed and replaced by a scar, and there were a number of TNF + inflammatory cells (black brown) in the scar; (D3) the DSS + B. subtilis -fermented milk group: the recovered epithelium showed faint yellow, suggesting low TNF expression. (E) The IL-10 immunohistochemistry staining of the small intestinal mucosa: (E1) the normal small intestinal mucosa: the IL-10 staining dispersed in the villi and the crypts with faint yellow, suggesting low-level expression of IL-10; (E2) the DSS group, the residual epithelium and the crypts were light brown, suggesting mid-level of IL-10 expression; (E3) the DSS + B. subtilis -fermented milk group: the dark brown staining of the regenerative epithelium represented high-level expression of IL-10. (F) The IL-10 immunohistochemistry staining of the colonic mucosa: (F1) the normal group: the IL-10 staining dispersed in the glands with bright yellow, suggesting low-level expression of IL-10; (F2) the DSS group: there were few IL-10 + cells in the scars; (F3) the DSS + B. subtilis -fermented milk group, the dark brown staining of the epithelial cells represented high-level expression of IL-10. (G,H) Western blotting analysis for the expression of MPO, TNF, and IL-10 in the samples containing equivalent ileum and colon. The expression level of MPO, TNF, and IL-10 in the DSS group was significantly higher than that of the normal (control) group. The expression level of MPO and TNF in the DSS + B. subtilis -fermented milk (FM) group was significantly lower than that of the DSS group, while the expression level of IL-10 in the DSS + B. subtilis -fermented milk (FM) group was significantly higher than that of the DSS group ( n = 5, * represents p < 0.05, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rat-il-10-antibody-rp1016-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1016-ajtr0008-2210-f4.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg;Activation of STAT3 participates in the effects of PTX in attenuating lung injury. (A, B) The levels of TNF-α (A) and IL-10 (B) in BAL fluids collected from rats in were measured. (C) Lung tissues were subjected to Western blot analysis, and the density of each band was normalized to GAPDH. *P < 0.05 and **P < 0.001 indicate a significant difference. ‡P < 0.05 and ‡‡P < 0.001 indicate a significant reduction; while #P < 0.05 and ##P < 0.001, a significant increase from vehicle-treated IAC rats.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4891433/&orig_host=www.ncbi.nlm.nih.gov'>27347328</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1016-il10-primary-antibodies-wb-testing-1.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg; Western blot analysis of IL10 using anti-IL10 antibody (RP1016). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant rat IL10 protein 10 ng,<br> Lane 2: recombinant rat IL10 protein 5 ng,<br> Lane 3: recombinant rat IL10 protein 1 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # RP1016) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1016-il10-primary-antibodies-ihc-testing-2_1.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg; IHC analysis of IL10 using anti-IL10 antibody (RP1016). <br> IL10 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL10 Antibody (RP1016) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-il-18-antibody-rp1017-boster.html2026-04-01T05:01:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-thnov10p9702g003.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Fgl2 deficiency attenuated liver inflammatory injury in NASH mice. In MCD-fed or HFD-fed WT and fgl2-/- mice, HE staining was performed to detect histological changes in the liver (A). The NAFLD activity score was evaluated (B). BMDMs were isolated from WT or fgl2-/- mice and injected into macrophage-depleted WT NASH mice (C). Histological changes were detected by HE staining (D, arrows indicate inflammatory infiltration). Serum ALT, AST, LDH (E) and fasting glucose (F) were tested by an automatic biochemical analyzer (n=10 in each group). The levels of serum insulin were tested by an ELISA kit (F). The levels of the proinflammatory cytokines TNF-α, MCP-1, IL-6, IL-1β and IL-18 in the liver were tested by ELISAs (G). For bar graphs, n=6-10 in each group. The data represent the mean ± SD from at least three independent experiments. Statistical differences were determined by two-way ANOVA. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7449923/&orig_host=www.ncbi.nlm.nih.gov'>32863955</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-thnov10p9702g005.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Fgl2 deficiency reduced lipid accumulation in hepatocytes by inhibiting the secretion of proinflammatory cytokines in macrophages. BMDMs from WT and fgl2-/- mice were stimulated with LPS (100 ng/ml) or FFA (800 μmol/L). The levels of proinflammatory cytokines, including TNF-α, MCP-1, IL-6, IL-1β and IL-18, in the supernatant of cell cultures were tested by ELISAs (A). Primary hepatocytes were isolated from C57BL/6J livers and incubated with LPS- or FFA-BMDM-CM for 24 hours. The brief experimental procedure is shown in a diagram. Oil red O staining was used to detect fat deposition in primary hepatocytes after treatment with BMDM-CM (B). Then, the mRNA levels of genes involved in lipogenesis (Fasn, SREBP-2) (C) or lipolysis (PPARα, CPT1A) (D) in primary hepatocytes were tested by real-time PCR. For bar graphs, n=6 in each group. The data represent the mean ± SD from at least three independent experiments. Statistical differences were determined by two-way ANOVA. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7449923/&orig_host=www.ncbi.nlm.nih.gov'>32863955</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-thnov10p9702g007.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Fgl2 disruption inhibited activation of the NLRP3 inflammasome in NASH. Total protein was obtained from liver tissues of MCD-fed or HFD-fed WT and fgl2-/- mice. MCS-fed and chow-fed mice were used as controls. NLRP3, pro-caspase-1, cleaved caspase-1 (caspase-1 p10), pro-IL-1β, mature IL-1β and IL-18 were analyzed by western blotting (A). BMDMs from WT and fgl2-/- mice were stimulated with LPS or FFA and tested for inflammasomes by western blotting (B). Image density was quantified using ImageLab software. For bar graphs, n=6 in each group. The data represent the mean ± SD from at least three independent experiments. Statistical differences were determined by two-way ANOVA. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7449923/&orig_host=www.ncbi.nlm.nih.gov'>32863955</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-wjg-11-4524-g001.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Gut ischemia/reperfusion results in increased levels of IL-18 in serum and lung. (A and B) The circulating levels of IL-18 after gut ischemia/reperfusion. Mice were subjected to gut ischemia/reperfusion or sham. Serum IL-18 at the indicated times after reperfusion was collected, part of which was immunoprecipitated with polyclonal rabbit anti-murine IL-18 antibody and analyzed by immunoblotting (A), and another part was assayed with ELISA (B) ( n = 5). (C) Gut ischemia/reperfusion results in elevated levels of IL-18 in lung tissue. Immunohistochemistry of lung sections obtained from normal (a, ×100, and b, ×200), 1 h (c) or 3 h (d) I/R model. The positive staining for IL-18 shows as dark brown. Magnification (c and d) ×200.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4398702/&orig_host=www.ncbi.nlm.nih.gov'>16052682</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-wjg-11-4524-g002.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;The effect of exogenous IL-18 on the ischemia/reperfusion-induced lung injury. A: IL-18 injection further remarkably enhanced the neutrophil sequestration in lung. After indicated times of reperfusion, the MPO activity was determined in lung tissue ( n = 5), a P <0.05 vs I/R. B: Comparison of pulmonary histopathology. Lungs from IL-18-nontreated (a, for 1 h of reperfusion and b, for 3 h) or IL-18-treated (c, for 1 h and d, for 3 h of reperfusion) mice subjected to gut I/R and stained with HE. Magnification ×100.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4398702/&orig_host=www.ncbi.nlm.nih.gov'>16052682</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-wjg-11-4524-g003.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;The effect of anti-IL-18 antibody on the ischemia-induced lung injury. A: Anti-IL-18 antibody injection remarkably inhibited the lung MPO activity. After 3 h of reperfusion, the MPO activity was determined in lung tissues ( n = 5), a P <0.05 vs others. B: Comparison of pulmonary histopathology. Lungs from ischemic mice (a, for 3 h of reperfusion), and ischemic mice treated with IL-18Ab (b, for 3 h of reperfusion) and stained with HE. Magnification ×100.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4398702/&orig_host=www.ncbi.nlm.nih.gov'>16052682</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-il18-primary-antibodies-wb-testing-1_1.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg; Western blot analysis of IL18 using anti-IL18 antibody (RP1017). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse IL18 recombinant protein,<br> Lane 2: mouse J774A.1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # RP1017) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1017-il18-primary-antibodies-ihc-testing-2.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg; IHC analysis of IL18 using anti-IL18 antibody (RP1017). <br> IL18 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (RP1017) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-ceacam5-antibody-rp1018-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1018-1_1.jpgAnti-CEACAM5 Antibody Picoband&reg; Western blot analysis of CEA using anti-CEA antibody (RP1018).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human SW620 whole cell lysates&#44; <br> Lane 2: human Caco-2 whole cell lysates&#44; <br> Lane 3: mouse small intestine tissue lysates&#44; <br> Lane 4: mouse stomach tissue lysates&#44;<br> Lane 5: mouse lung tissue lysates&#44;<br> Lane 6: mouse liver tissue lysates&#44;<br> Lane 7: mouse NIH3T3 whole cell lysates&#44;<br> Lane 8: mouse HEPA1-6 whole cell lysates&#44;<br> Lane 9: mouse SP20 whole cell lysates&#44;<br> Lane 10: rat RH35 whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEA antigen affinity purified polyclonal antibody (Catalog # RP1018) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEA at approximately 120-200KD. The expected band size for CEA is at 77KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1018-cea-primary-antibodies-ihc-testing-2.jpgAnti-CEACAM5 Antibody Picoband&reg; IHC analysis of CEA using anti-CEA antibody (RP1018). <br> CEA was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CEA Antibody (RP1018) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-il-17-antibody-rp1019-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1019-1-WB-anti-il-17-antibody.jpgAnti-IL17/IL17A Antibody Picoband&reg;Figure. Western blot analysis of IL-17 using anti-IL-17 antibody (RP1019). <br><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br><br> Lane : Recombinant Mouse IL-17 Protein 0.5ng<br><br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-17 antigen affinity purified polyclonal antibody (Catalog # RP1019) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-17 at approximately 30KD. The expected band size for IL-17 is at 30KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-mif-antibody-rp1020-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1020-mif-primary-antibodies-wb-testing-1.jpgAnti-MIF Antibody Picoband&reg; Western blot analysis of MIF using anti-MIF antibody (RP1020). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (Catalog # RP1020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-transferrin-antibody-rp1022-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1022-tf-primary-antibodies-ihc-testing-3.jpgAnti-Transferrin/TF Antibody Picoband&reg;IHC analysis of Transferrin/TF using anti-Transferrin/TF antibody (RP1022). <br>Transferrin/TF was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Transferrin/TF Antibody (RP1022) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1022-tf-primary-antibodies-wb-testing-1.jpgAnti-Transferrin/TF Antibody Picoband&reg; Western blot analysis of Transferrin/TF using anti-Transferrin/TF antibody (RP1022). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat RH-35 whole cell lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse Hepa1/6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Transferrin/TF antigen affinity purified polyclonal antibody (Catalog # RP1022) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Transferrin/TF at approximately 77 kDa. The expected band size for Transferrin/TF is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1022-tf-primary-antibodies-ihc-testing-2_1.jpgAnti-Transferrin/TF Antibody Picoband&reg; IHC analysis of Transferrin/TF using anti-Transferrin/TF antibody (RP1022). <br> Transferrin/TF was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Transferrin/TF Antibody (RP1022) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1022-tf-primary-antibodies-fcm-testing-3.jpgAnti-Transferrin/TF Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Transferrin/TF antibody (RP1022). <br> Overlay histogram showing HepG2 cells stained with RP1022 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Transferrin/TF Antibody (RP1022, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-growth-hormone-antibody-rp1023-boster.html2026-03-25T05:21:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1023-2-IHC-anti-growth-hormone-antibody.jpgAnti-Growth Hormone/GH1 Antibody Picoband&reg;Anti-human Growth Hormone antibody&#44; RP1023&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1023-1_1.jpgAnti-Growth Hormone/GH1 Antibody Picoband&reg; Western blot analysis of GH1 using anti-GH1 antibody (RP1023). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GH1 antigen affinity purified polyclonal antibody (Catalog # RP1023) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 22KD. The expected band size for GH1 is at 25KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-growth-hormone-antibody-rp1024-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1024-1-WB-anti-growth-hormone-antibody.jpgAnti-Growth Hormone/GH1 Antibody Picoband&reg;Anti-mouse Growth Hormone antibody&#44; RP1024&#44; Western blotting<br>Lane 1: Recombinant Mouse GH Protein 10ng<br>Lane 2: Recombinant Mouse GH Protein 5ng<br>Lane 3: Recombinant Mouse GH Protein 2https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1024-dddt_a_12469766_f0007_c.jpgAnti-Growth Hormone/GH1 Antibody Picoband&reg;Verification results of the WB and ELISA test. Notes: (A) Test procedure; (B) WB strip map; (C) WB gray value; (D) ELISA test results. The data are shown as the means ± SDs (n = 5). *P < 0.05, **P < 0.01 and ***P < 0.001 denote significant differences from the control group. #P < 0.05, ##P < 0.01 and ###P < 0.001 denote significant differences from the Model group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/DDDT.S537918'>40859969</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-ngf-antibody-rp1025-boster.html2026-03-24T05:03:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1025-1-WB-anti-ngf-ngf-beta-antibody.jpgAnti-Beta-nerve growth factor NGF Antibody Picoband&reg;Anti-human NGF antibody&#44; RP1025&#44; Western blotting<br>Lane 1: Recombinant Human NGFB Protein 10ng<br>Lane 2: Recombinant Human NGFB Protein 5ng<br>Lane 3: Recombinant Human NGFB Protein 2 https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-survivin-antibody-rp1026-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1026-birc5-primary-antibodies-wb-testing-1.jpgAnti-survivin/BIRC5 Antibody Picoband&reg; Western blot analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin/BIRC5 antigen affinity purified polyclonal antibody (Catalog # RP1026) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin/BIRC5 at approximately 16 kDa. The expected band size for Survivin/BIRC5 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1026-birc5-primary-antibodies-ihc-testing-2.jpgAnti-survivin/BIRC5 Antibody Picoband&reg; IHC analysis of Survivin/BIRC5 using anti-Survivin/BIRC5 antibody (RP1026). <br> Survivin/BIRC5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/BIRC5 Antibody (RP1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-angiostatin-k1-3-antibody-rp1027-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1027-plg-primary-antibodies-wb-testing-1.jpgAnti-Angiostatin K1-3/PLG Antibody Picoband&reg; Western blot analysis of Angiostatin K1-3 using anti-Angiostatin K1-3 antibody (RP1027). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: human CACO-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiostatin K1-3 antigen affinity purified polyclonal antibody (Catalog # RP1027) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiostatin K1-3 at approximately 90-100 kDa. The expected band size for Angiostatin K1-3 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1027-2.jpgAnti-Angiostatin K1-3/PLG Antibody Picoband&reg; IHC analysis of Angiostatin K1-3 using anti-Angiostatin K1-3 antibody (RP1027).<br> Angiostatin K1-3 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Angiostatin K1-3 Antibody (RP1027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1027-3.jpgAnti-Angiostatin K1-3/PLG Antibody Picoband&reg; IHC analysis of Angiostatin K1-3 using anti-Angiostatin K1-3 antibody (RP1027).<br> Angiostatin K1-3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Angiostatin K1-3 Antibody (RP1027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abi1-antibody-pa1001-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1001-abi1-primary-antibodies-wb-testing-1.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; Western blot analysis of ABI1 using anti-ABI1 antibody (PA1001). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human U87 whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse Neuro-2a whole cell lysates,<br> Lane 8: mouse C2C12 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI1 antigen affinity purified polyclonal antibody (Catalog # PA1001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABI1 at approximately 60-65 kDa. The expected band size for ABI1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1001-abi1-primary-antibodies-ihc-testing-2.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IHC analysis of ABI1 using anti-ABI1 antibody (PA1001). <br> ABI1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABI1 Antibody (PA1001) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1001-abi1-primary-antibodies-if-testing-5.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IF analysis of ABI1 using anti-ABI1 antibody (PA1001). <br> ABI1 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ABI1 Antibody (PA1001) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1001-abi1-primary-antibodies-ihc-testing-3.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IHC analysis of ABI1 using anti-ABI1 antibody (PA1001). <br> ABI1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABI1 Antibody (PA1001) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1001-abi1-primary-antibodies-if-testing-6.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IF analysis of ABI1 using anti-ABI1 antibody (PA1001). <br> ABI1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ABI1 Antibody (PA1001) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1001-abi1-primary-antibodies-if-testing-4.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IF analysis of ABI1 using anti-ABI1 antibody (PA1001). <br> ABI1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABI1 Antibody (PA1001) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1001-abi1-primary-antibodies-fcm-testing-7.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-ABI1 antibody (PA1001). <br> Overlay histogram showing MCF-7 cells stained with PA1001 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABI1 Antibody (PA1001, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alkaline-phosphatase-antibody-pa1004-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1004-alpl-primary-antibodies-ihc-testing-3.jpgAnti-Alkaline Phosphatase/ALPL Antibody Picoband&reg;IHC analysis of ALPL using anti-ALPL antibody (PA1004). <br> ALPL was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALPL Antibody (PA1004) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1004-alpl-primary-antibodies-wb-testing-1.jpgAnti-Alkaline Phosphatase/ALPL Antibody Picoband&reg; Western blot analysis of ALPL using anti-ALPL antibody (PA1004). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALPL antigen affinity purified polyclonal antibody (Catalog # PA1004) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALPL at approximately 80 kDa. The expected band size for ALPL is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1004-alpl-primary-antibodies-ihc-testing-4.jpgAnti-Alkaline Phosphatase/ALPL Antibody Picoband&reg;IHC analysis of ALPL using anti-ALPL antibody (PA1004). <br> ALPL was detected in a paraffin-embedded section of human acinic cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALPL Antibody (PA1004) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1004-alpl-primary-antibodies-ihc-testing-2.jpgAnti-Alkaline Phosphatase/ALPL Antibody Picoband&reg; IHC analysis of ALPL using anti-ALPL antibody (PA1004). <br> ALPL was detected in a paraffin-embedded section of human parotid acinar cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALPL Antibody (PA1004) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1004-alpl-primary-antibodies-if-testing-1.jpgAnti-Alkaline Phosphatase/ALPL Antibody Picoband&reg;IF analysis of ALPL using anti-ALPL antibody (PA1004). <br> ALPL was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALPL Antibody (PA1004) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alkaline-phosphatase-antibody-pa1004-1-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1004-1-1-WB-anti-alkaline-phosphatase-antibody.jpgAnti-Alkaline Phosphatase/ALPL Antibody Picoband&reg;All lanes: Anti ALPL (PA1004-1) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: Rat Brain Tissue Lysate at 50ug<br>Predicted bind size: 57KD<br>Observed bind size: 57KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alkaline-phosphatase-antibody-pa1004-2-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1004-2-1-WB-anti-alkaline-phosphatase-antibody.jpgAnti-Alkaline Phosphatase/ALPL Antibody Picoband&reg;All lanes: Anti ALPL (PA1004-2) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: HT1080 Whole Cell Lysate at 40ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 57KD<br>Observed bind size: 57KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angiopoietin-2-antibody-pa1005-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1005-1-WB-anti-angiopoietin-2-antibody.jpgAnti-Angiopoietin 2/ANGPT2 Antibody Picoband&reg;Anti-Angiopoietin 2 antibody&#44; PA1005&#44; Western blotting<br>Lane 1: Recombinant Human ANG2 Protein 10ng<br>Lane 2: Recombinant Human ANG2 Protein 5ng<br>Lane 3: Recombinant Human ANG2 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a1-antibody-pa1006-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1006-2-IHC-anti-annexin-a1-antibody.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg;Anti-Annexin A1 antibody&#44; PA1006&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1006-anxa1-primary-antibodies-wb-testing-1.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg; Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (PA1006). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: monkey COS-7 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A1/ANXA1 antigen affinity purified polyclonal antibody (Catalog # PA1006) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 39KD. The expected band size for Annexin A1/ANXA1 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1006-fonc-10-537322-g003.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg;Germacrone induced apoptosis in gastric cancer cells. (A–D) Annexin IV-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining was used to assess the effect of germacrone on apoptosis by flow cytometry (FCM). (E) Hoechst 33258 staining was used to detect apoptosis. (F, G) Changes in the expression levels of BAX, Bcl-2, caspase-3, and cleaved caspase-3 were detected by western blot. (H, I) Changes in the BAX/Bcl-2 and cleaved caspase-3/caspase-3 ratios were analyzed after germacrone treatment. Data are the means ± SD of three experiments. * P < 0.05; ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.537322/full'>33244453</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1006-fonc-10-537322-g005.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg;Label-free proteomic and bioinformatic analysis of proteins associated with the cell cycle, apoptosis, and autophagy. (A–C) The DAVID database was used for analysis of molecular function, cellular component, and biological process. (D) A heat map was constructed based on the abundance of 111 proteins. D1, D2, and D3 represent the DMSO 1, DMSO 2, and DMSO 3 groups; G1, G2, and G3 represent the germacrone 1, germacrone 2, and germacrone 3 groups. (E) A protein interaction network was constructed in Cytoscape based on the information provided by the STRING database. Red indicates increased expression of the protein in the germacrone group; green indicates reduced expression of the protein in the germacrone group. (F, G) . The expression of HBXIP, HSP70, and ANXA1 was detected to verify the accuracy of the proteomics results.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.537322/full'>33244453</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1006-fonc-10-537322-g007.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg;Overexpression of HBXIP regulated autophagy and reversed the germacrone-induced cell cycle arrest and apoptosis. (A–D) Propidium iodide (PI) staining was used for flow cytometric (FCM) analysis of the effect of germacrone and overexpression of HBXIP on the cell cycle. (E–H) Annexin IV-fluorescein isothiocyanate (FITC)/PI staining was used to detect the effect of germacrone and overexpression of HBXIP on apoptosis by FCM. (I, J) The expression of HBXIP, p-62, LC3I, and LC3II was detected by western blot. (K) A schematic of the effect of germacrone on gastric cancer cells. Germacrone inhibits gastric cancer cell proliferation involving HBXIP-mediated regulation of the cell cycle, apoptosis, and autophagy. Data are the means ± SD of three experiments. * P < 0.05; ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.537322/full'>33244453</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1006-3-IHC-anti-annexin-a1-antibody.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg;Anti-Annexin A1 antibody&#44; PA1006&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1006-anxa1-primary-antibodies-if-testing-4.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg; IF analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (PA1006). <br> Annexin A1/ANXA1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Annexin A1/ANXA1 Antibody (PA1006) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1006-anxa1-primary-antibodies-fcm-testing-5.pngAnti-Annexin A1/ANXA1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-Annexin A1/ANXA1 antibody (PA1006). <br>Overlay histogram showing A431 cells stained with PA1006 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin A1/ANXA1 Antibody (PA1006, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-iv-antibody-pa1007-1-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1007-1-anxa4-primary-antibodies-wb-testing-1.jpgAnti-Annexin IV/ANXA4 Antibody Picoband&reg; Western blot analysis of Annexin IV/ANXA4 using anti-Annexin IV/ANXA4 antibody (PA1007-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates, <br> Lane 2: human SiHa whole cell lysates, <br> Lane 3: human Hacat whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human U251 whole cell lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: human PC-3 whole cell lysates, <br> Lane 8: rat lung tissue lysates, <br> Lane 9: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin IV/ANXA4 antigen affinity purified polyclonal antibody (Catalog # PA1007-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin IV/ANXA4 at approximately 36 kDa. The expected band size for Annexin IV/ANXA4 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1007-1-2-IHC-anti-annexin-iv-antibody.jpgAnti-Annexin IV/ANXA4 Antibody Picoband&reg; IHC analysis of Annexin IV/ANXA4 using anti-Annexin IV/ANXA4 antibody (PA1007-1). <br> Annexin IV/ANXA4 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin IV/ANXA4 Antibody (PA1007-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1007-1-3_1-IHC-anti-annexin-iv-antibody.jpgAnti-Annexin IV/ANXA4 Antibody Picoband&reg; IHC analysis of Annexin IV/ANXA4 using anti-Annexin IV/ANXA4 antibody (PA1007-1). <br> Annexin IV/ANXA4 was detected in a paraffin-embedded section of Rat Pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin IV/ANXA4 Antibody (PA1007-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-v-antibody-pa1008-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1008-2-IHC-anti-annexin-v-antibody.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg;Anti-Annexin V antibody&#44; PA1008&#44; IHC(P)<br>IHC(P): Rat Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1008-3-IF-anti-annexin-v-antibody.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg;Anti-Annexin V antibody&#44; PA1008&#44; ICC<br>ICC: HCT116 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1008-4-IF-anti-annexin-v-antibody.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg;Anti-Annexin V antibody&#44; PA1008&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1008-1_1.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg;Anti-Annexin V antibody&#44; PA1008&#44; Western blotting<br>All lanes: Anti Annexin V (PA1008) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Lane 3: Rat Ovary Tissue Lysate at 50ug<br>Lane 4: Rat Lung Tissue Lysate at 50ug<br>Lane 5: MCF-7 Whole Cell Lysate at 40ug<br>Lane 6: SMMC Whole Cell Lysate at 40ug<br>Lane 7: A549 Whole Cell Lysate at 40ug<br>Lane 8: JURKAT Whole Cell Lysate at 40ug<br>Lane 9: SGC Whole Cell Lysate at 40ug<br>Lane 10: HT1080 Whole Cell Lysate at 40ug<br>Predicted bind size: 36KD<br>Observed bind size: 36KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apoptosis-inhibitor-5-antibody-pa1009-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1009-1_1.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; Western blot analysis of API5 using anti-API5 antibody (PA1009). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: Rat Cardiac Muscle Tissue Lysate Lane 2: Rat Brain Tissue Lysate,<br> Lane 3: Rat Testis Tissue Lysate,<br> Lane 4: Rat Placenta Tissue Lysate,<br> Lane 5: MCF-7 Cell Lysate,<br> Lane 6: HELA Cell Lysate,<br> Lane 7: CEM Cell Lysate,<br> Lane 8: SMMC Cell Lysate,<br> Lane 9: COLO320 Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-API5 antigen affinity purified polyclonal antibody (Catalog # PA1009) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for API5 at approximately 59 kDa. The expected band size for API5 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1009-2-IHC-anti-apoptosis-inhibitor-5-antibody.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; IHC analysis of API5 using anti-API5 antibody (PA1009). <br> API5 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-API5 Antibody (PA1009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1009-3-IF-anti-apoptosis-inhibitor-5-antibody.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; ICC analysis of API5 using anti-API5 antibody (PA1009). <br> API5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-API5 Antibody (PA1009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1009-4-IHC-anti-apoptosis-inhibitor-5-antibody.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; IHC analysis of API5 using anti-API5 antibody (PA1009). <br> API5 was detected in a frozen section of Rat Cardiac Muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-API5 Antibody (PA1009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1009-5.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; IHC analysis of API5 using anti-API5 antibody (PA1009). <br> API5 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-API5 Antibody (PA1009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1009-6.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; IHC analysis of API5 using anti-API5 antibody (PA1009). <br> API5 was detected in paraffin-embedded section of rat intestines tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-API5 Antibody (PA1009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1009-7.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; IHC analysis of API5 using anti-API5 antibody (PA1009). <br> API5 was detected in paraffin-embedded section of mouse intestines tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-API5 Antibody (PA1009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9128-api5-primary-antibodies-if-testing-8.jpgAnti-Apoptosis inhibitor 5/API5 Antibody Picoband&reg; IF analysis of API5 using anti-API5 antibody (PA1009). <br> API5 was detected in an immunocytochemical section of SK-OV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-API5 Antibody (PA1009) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-1-antibody-pa1010-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1010-aqp1-primary-antibodies-wb-testing-1.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; Western blot analysis of AQP1 using anti-AQP1 antibody (PA1010). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates,<br> Lane 2: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP1 antigen affinity purified polyclonal antibody (Catalog # PA1010) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP1 at approximately 29 kDa. The expected band size for AQP1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1010-aqp1-primary-antibodies-ihc-testing-2_1.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; IHC analysis of AQP1 using anti-AQP1 antibody (PA1010). <br> AQP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PA1010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1010-aqp1-primary-antibodies-ihc-testing-3_1.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; IHC analysis of AQP1 using anti-AQP1 antibody (PA1010). <br> AQP1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PA1010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1010-aqp1-primary-antibodies-ihc-testing-4.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; IHC analysis of AQP1 using anti-AQP1 antibody (PA1010). <br> AQP1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PA1010) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1010-aqp1-primary-antibodies-fcm-testing-5.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-AQP1 antibody (PA1010). <br> Overlay histogram showing Hela cells stained with PA1010 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP1 Antibody (PA1010, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bax-antibody-pa1013-1-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-bax-primary-antibodies-wb-testing-1.jpgAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Western blot analysis of BAX using anti-BAX antibody (PA1013-1). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAX antigen affinity purified polyclonal antibody (PA1013-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BAX at approximately 21 kDa. The expected band size for BAX is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-bax-primary-antibodies-ihc-testing-1.jpgAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;IHC analysis of BAX using anti-BAX antibody (PA1013-1). <br>BAX was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAX Antibody (PA1013-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-bax-primary-antibodies-ihc-testing-2.jpgAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;IHC analysis of BAX using anti-BAX antibody (PA1013-1). <br>BAX was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BAX Antibody (PA1013-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-bax-primary-antibodies-if-testing-1.jpgAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;IF analysis of BAX using anti-BAX antibody (PA1013-1). <br> BAX was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BAX Antibody (PA1013-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-bax-primary-antibodies-ip-testing-1.jpgAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Immunoprecipitating (IP) BAX in Hela whole cell lysate.<br> Western blot analysis of BAX using anti-BAX antibody (PA1013-1); <br> Lane 1: Hela whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-BAX antibody in Hela whole cell lysate;<br> Lane 3: anti-BAX antibody (2μg) + Hela whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BAX antigen affinity purified polyclonal antibody (PA1013-1) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for BAX at approximately 21 kDa. The expected band size for BAX is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-bax-primary-antibodies-fcm-testing-1.jpgAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Flow Cytometry analysis of MCF-7 cells using anti-BAX antibody (PA1013-1). <br>Overlay histogram showing MCF-7 cells stained with PA1013-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAX Antibody (PA1013-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-11658_2022_330_fig4_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;MiR-742-3p relieved hypoxia-induced H9c2 cell injury. H9c2 cells were transfected with or without miR-NC (50 nM) or miR-742-3p mimic (50 nM), and then treated with hypoxia. Untreated H9c2 cells were used as control. Cell viability, migrated and invaded cell numbers, and cell apoptosis rate were measured using CCK8 assay ( A ), transwell assay ( B , C ) and flow cytometry ( D ). E , F The protein levels of Bcl-2, Bax and Cleaved-caspase 3 were determined using WB analysis. All experiments were repeated three times. *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s11658-022-00330-y'>35346026</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-11658_2022_330_fig5_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Effects of circRbms1 silencing and miR-742-3p inhibitor on hypoxia-induced H9c2 cell injury. A After transfecting with anti-NC (50 nM) or anti- miR-742-3p (50 nM) into H9c2 cells, the expression of miR-742-3p was assessed by qRT-PCR. B–F H9c2 cells were transfected with si-NC (50 nM), si- circRbms1 (50 nM), si- circRbms1 (50 nM) + anti-NC (50 nM), or si- circRbms1 (50 nM) + anti- miR-742-3p (50 nM), and then treated with hypoxia. Untreated H9c2 cells were used as control. CCK8 assay ( B ), transwell assay ( C , D ), and flow cytometry ( E ) were employed to examine cell viability, migrated and invaded cell numbers, and cell apoptosis rate, respectively. F WB analysis was used to test the protein levels of Bcl-2, Bax, and Cleaved-caspase 3. All experiments were repeated three times. ** P < 0.01, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s11658-022-00330-y'>35346026</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-13046_2025_3365_fig6_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Exploration of the mechanism of cuproptosis induction. ( A ) After the transient overexpression of FDX1, a CCK-8 assay was used to detect changes in cellular resistance to cuproptosis. ( B ) After the transient overexpression of circKIAA1797, a CCK-8 assay was performed to detect changes in cellular resistance to cuproptosis. ( C ) Western blot analysis of Lip-DLAT, DLAT, LIAS, TOM20, and FDX1 protein expression after the stable silencing of circKIAA1797. ( D ) Immunofluorescence staining showed the colocalisation of the DLAT and FDX1 proteins. ( E ) Immunofluorescence staining was performed to detect the colocalisation of DLAT with the LIPT1 protein. ( F ) Western blot results of the Co-IP assay used to detect FDX1 binding to LIAS. ( G ) Western blot analysis of LIAS protein expression levels after the gradient overexpression of FDX1. ( H ) Western blot results of the Co-IP assay for detecting FDX1 binding to LIPT1. ( I ) Western blot results of Co-IP experiments for detecting FDX1 binding to DLAT. ( J ) Gradient overexpression of FDX1 followed by Western blot detection of DLAT and LIPT1 protein expression levels. ( K ) Western blot results of the Co-IP assay used to detect LIPT1 binding to DLAT. ( L ) After the overexpression of FDX1, Co-IP experiments were performed to detect changes in the ability of lipoic acid to bind DLAT. ( M ) Cells were treated with 0 nM, 50 nM, 100 nM and 200 nM elesclomol-Cu, and Western blot was performed to examine the protein expression of LIAS and FDX1. ( N ) circKIAA1797 stably transfected cells were treated with 50 nM elesclomol-Cu, and FDX1 protein expression was examined by Western blot. ( O ) Western blot analysis of BAX and Bcl2 protein expression in cell lines with stable circKIAA1797 silencing. ( P ) Flow cytometry detection of changes in the mitochondrial membrane potential after the transient silencing of circKIAA1797. ( Q ) Fluorescence microscopy comparing the changes in JC monomer and multimer levels after the transient silencing of circKIAA1797. ( R ) After the overexpression of Bcl2, Western blot assays were performed to detect Lip-DLAT, DLAT, LIAS, and FDX1 protein expression. ( S ) After the overexpression of Bcl2, a CCK-8 assay was performed to detect changes in cellular resistance to cuproptosis <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-025-03365-z'>40176113</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-11658_2022_330_fig2_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Knockdown of circRbms1 alleviated hypoxia-induced H9c2 cell injury. A , B qRT-PCR was used to assess circRbms1 expression to evaluate the transfection efficiency of si-circRbms1 (50 nM) or circRbms1 overexpression vector (4.0 µg) in H9c2 cells. C – G H9c2 cells were transfected with or without si-NC (50 nM), si- circRbms1 (50 nM), vector (4.0 µg) or circRbms1 (4.0 µg), and then treated with hypoxia. Untreated H9c2 cells were used as control. CCK8 assay ( C ), transwell assay ( D , E ) and flow cytometry ( F ) were used to determine cell viability, migrated and invaded cell numbers, and cell apoptosis rate, respectively. G WB analysis was performed to test the protein levels of Bcl-2, Bax, and Cleaved-caspase 3. All experiments were repeated three times. ** P < 0.01, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s11658-022-00330-y'>35346026</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-40364_2022_407_fig5_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;MiR-148a-3p Reversed the Regulatory Effect of circ_0009043 on A549 and HCC827 cells. A549 and HCC827 cells were transfected with shCirc, shCirc + NC, or shCirc + miR-148a-3p inhibitor. (A) MiR-148a-3p mRNA levels in A549 and HCC827 were determined with RT-qPCR assay. B Viability in A549 and HCC827 cells at 0, 24, and 48 h. C EdU assay to detect A549 and HCC827 cell proliferation. (D) FSC assay to detect A549 and HCC827 cell apoptosis. E Protein levels of Bax and Bcl-2 in A549 and HCC827 cells with the indicated transfection were determined by western blot. GAPDH is a loading control. Data are presented as mean ± standard deviation. ** P < 0.01; *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40364-022-00407-y'>35974419</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-40364_2022_407_fig7_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Overexpression of DNAJB4 inhibits the proliferation but promotes apoptosis abilities of NSCLC cells. A DNAJB4 mRNA expression in HCC827 and A549 cells transfected with pcDNA4.0 vector, pcDNA4.0- DNAJB4 vector, CTRL‑shRNA, or DNAJB4 ‑shRNA were determined by RT-qPCR. B DNAJB4 protein expression in HCC827 and A549 cells transfected with indicated vectors, which divided into five groups, including Control, OE-CTRL, OE-DNAJB4, shCTRL and shDNAJB4. C CCK8 assay was used to compare the cell proliferation of CTRL, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups in HCC827 and A549 cells. D Edu assay of Control, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups in HCC827 and A549 cells, respectively. E FSC assay to detect cell apoptosis of Control, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups in HCC827 and A549 cells. F Expression level of Bax, Bcl-2 and Cytochrome C in the HCC827 and A549 cells of the 5 groups (Control, OE-CTRL, OE- DNAJB4, shCTRL and sh DNAJB4 groups) were determined by western blotting. Control negative control, OE over expression. Data represent mean values ± SD from three replicates of each sample; ** P < 0.01, *** P < 0.001, **** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40364-022-00407-y'>35974419</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12951_2023_2204_fig8_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;PDGF-BB promotes MSC migration and protects MSCs against apoptosis via PI3K/Akt signaling. ( A - B ) Western blot analysis of total and phosphorylated PI3K, Akt, and ERK in MSCs with or without PDGF-BB treatment. PDGF-BB, 50 ng/ml. Values are the mean ± SEM. Significant differences were determined by Student’s t test. N = 6/group. *P < 0.05 vs. vehicle. ( C - D ) Western blot analysis of CXCR4 in MSCs. LY294002, 10 µM, U0126, 10 µM, 2 h before PDGF-BB treatment. Values are the mean ± SEM. Significant differences were determined by using one-way ANOVA. N = 6/group. *p < 0.01 vs. vehicle; # p < 0.01 vs. PDGF-BB; & p < 0.01 vs. PDGF-BB/LY294002. ( E - F ). MSC migratory capacities were evaluated by Transwell assay. LY294002, 50 µM. AMD3100, 44 nM. PDGF-BB, 50 ng/ml. Bar, 100 μm. Values are the mean ± SEM. Significant differences were determined by using one-way ANOVA. N = 6/group. *p < 0.01 vs. vehicle; # p < 0.01 vs. PDGF-BB; & p < 0.01 vs. PDGF-BB/AMD3100. ( G - H ). MSC apoptosis was evaluated by TUNEL assay. Bar, 50 μm. ( I - J ) Western blot analysis of activated caspase-3 in MSCs. ( K - L ) Bax and BCL-2 mRNA expression levels were determined by RT‒PCR in MSCs. H 2 O 2 , 200 µM, 6 h. LY294002, 30 µM. Values are the mean ± SEM. Significant differences were determined by using one-way ANOVA. N = 6/group. *p < 0.01 vs. vehicle; # p < 0.01 vs. H 2 O 2 ; & p < 0.01 vs. H 2 O 2 /PDGF-BB <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-023-02204-7'>38102643</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12276_2022_735_fig6_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;IGF2BP3 regulates RCC2 expression in an m6A-dependent manner. a , b RCC2 expression was positively correlated with IGF2BP3 expression in the GSE37642 and TARGET datasets. c , d Kaplan–Meier survival analysis revealed that high RCC2 expression indicated a poor prognosis in AML patients. e , f Protein expression level of RCC2 following knockdown or overexpression of IGF2BP3 in HL-60 and KG-1 cells. g , h The interference efficiency of the siRNAs was evaluated to confirm the feasibility of the siRNAs, and si-RCC2#2 was found to be effective in reducing RCC2 expression. i , j Apoptosis was detected by flow cytometry. RCC2 deficiency promoted the induction of apoptosis by IGF2BP3 overexpression. k The mRNA of RCC2 was enriched by the anti-IGF2BP3 antibody compared to IgG in the HL-60 and KG-1 cell lines. l The mRNA of RCC2 was enriched by the m6A-specific antibody compared to IgG in the HL-60 and KG-1 cell lines. m Overexpression of IGF2BP3 restored the increases in the levels of proapoptotic proteins (Bax and cleaved Caspase 3) caused by silencing RCC2, and the level of the antiapoptotic protein Bcl-2 was slightly decreased. n The potential m6A sites in RCC2 were predicted by SRAMP. The different colored lines indicate different confidence levels. o , p Loss of IGF2BP3 reduced RCC2 stability in HL-60 and KG-1 cells. Transfected cells were treated with 5 µg/ml actinomycin D for 0 h, 3 h, or 6 hours prior to RNA extraction. * P < 0.05; ** P < 0.01; *** P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs12276-022-00735-x'>35217832</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12951_2023_2204_fig6_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Transplantation of PDGF-BB-primed MSCs via UTMD reduces cardiomyocyte apoptosis and improves angiogenesis in rat hearts post-MI. ( A ) Representative images of TUNEL-positive cardiomyocytes in the ischemic area 30 days after MI. Apoptotic nuclei were identified as TUNEL positive (green fluorescence), and total nuclei were identified by DAPI counterstaining (blue fluorescence). Myocardium was stained using a monoclonal antibody against cardiac troponin I (red fluorescent). Bar, 20 μm. ( B ). Quantification of TUNEL-positive cardiomyocytes. ( C - F ) Western blotting of activated caspase 3, Bax, and BCL-2 in the ischemic heart. GAPDH was used as a loading control. ( G ) Representative images of CD31 staining and α-SMA staining in the ischemic hearts of rats 30 days post-MI. Bar, 20 μm. ( H ) Quantitative analysis of the capillary density in the ischemic heart. (I) Quantitative analysis of the arteriole density in the ischemic heart. ( J - M ) Protein expression of VEGF, bFGF and IGF-1 determined by Western blotting in ischemic myocardium, with GAPDH as the internal control. Values are the mean ± SEM. Significant differences were determined by using one-way ANOVA. N = 5/group. *p < 0.01 vs. MI; # p < 0.01 vs. MI-MSC-vehicle <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-023-02204-7'>38102643</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-40364_2022_407_fig2_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Overexpression of circ_0009043 inhibits the proliferation, while accelerates apoptosis of NSCLC cells. A circ_0009043 mRNA expression in HCC827 and A549 cells transfected with pcDNA4.0 vector (OE-CTRL), pcDNA4.0- circ_0009043 vector (OE-Circ), CTRL‑shRNA (shCTRL), or circ_0009043‑shRNA (shCirc) were determined by RT-qPCR. B CCK8 assay was used to compare the cell proliferation of Control, OE-CTRL, OE-Circ, shCTRL, and shCirc groups in HCC827 and A549 cells. C Edu assay of Control, OE-CTRL, OE-Circ, shCTRL, and shCirc groups in HCC827 and A549 cells. D-E The ratio of apoptosis in the HCC827 and A549 cells transfected with indicated vectors, which consisting of the OE-CTRL, OE- Circ, shCTRL and shCirc groups were detected by flow cytometry. Comparison of the ratio of apoptosis in the afore mentioned 5 groups. Each bar indicates the mean apoptosis rate ± standard deviation per group. F-G Expression level of Bax, Bcl-2 and Cytochrome C in the HCC827 and A549 cells of the 5 groups (CTRL, OE-CTRL, OE- Circ, shCTRL and shCirc groups) were determined by western blotting. Data was normalized to GAPDH. All results were representative of three separate experiments. Data represent mean values ± SD from three replicates of each sample; * P < 0.05, ** P < 0.01, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40364-022-00407-y'>35974419</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12967_2024_5423_fig6_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;circSORBS1 inhibits lung cancer development through the RUFY3/YWHAE/BAD/BCL2 pathway. A , B qPCR detection of the transient overexpression efficiency of BCL2 mRNA in H226 versus H1299 cells. C , D . Western blot analysis of YWHAE protein expression after transient silencing and overexpression of RUFY3 mRNA and grey value analysis. E A CCK-8 assay was used to detect the viability of H1299 and H226 cells after transfection with BCL2. F , G Flow cytometry was used to detect the apoptotic capacity after BCL2 backfilling. H circSORBS1 acts as a miR-6779-5p sponge and indirectly inhibits RUFY3 mRNA degradation, directly binds to RUFY3 mRNA and enhances its stability, which in turn increases RUFY3 protein expression, activates the YWHAE/BAD/BCL2 apoptotic signalling pathway, and inhibits lung cancer progression <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05423-0'>38915053</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12967_2024_5423_fig5_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;circSORBS1 regulates apoptosis via the RUFY3/YWHAE pathway. A KEGG pathway analysis of genes downstream of RUFY3. B–E Correlation analysis of circSORBS1 with RUFY3 and key proteins of the apoptotic pathways YWHAE, BAD, and BCL2 in the UCSC database. F Results of the Western blot analysis for Co-IP experiments. G IF was used to detect the colocalization of the BCL2 protein with the BAD protein. H IF was used to detect the colocalization of YWHAE with the BAD protein. I Western blot analysis of YWHAE and RUFY3 protein expression after transient RUFY3 silencing and overexpression. J Western blot analysis of YWHAE and RUFY3 protein expression after transient circSORBS1 silencing and overexpression. K–R Immunohistochemistry was performed to detect the protein expression levels of RUFY3, YWHAE, BAD, and BCL2 in nude mouse tumours. S Western blot analysis of RUFY3 protein expression in the tumour tissue of nude mice <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05423-0'>38915053</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12967_2024_5423_fig3_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;circSORBS1 inhibits lung cancer development in vivo. A Construction of a circSORBS1 stable overexpression cell line by lentiviral transfection of H226 cells. B qPCR detection of circSORBS1 overexpression efficiency in H226 cells stably overexpressing circSORBS1. C , D Nude tumour formation experiments and tumour size determination. E Statistical analysis of tumour weight in nude mice. F Growth of the nude mice. G – N Immunohistochemical analysis of the protein expression of Ki67, CDK4, BAX, and E-cadherin in nude tumours <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05423-0'>38915053</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-11658_2022_330_fig7_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Effects of miR-742-3p and FOXO1 on hypoxia-induced H9c2 cell injury. A H9c2 cells were transfected with pcDNA3.1 and pcDNA3.1- FOXO1 , and the protein expression of FOXO1 was detected by WB analysis. B – F H9c2 cells were transfected with miR-NC (50 nM), miR-742-3p (50 nM), miR-742-3p (50 nM) + pcDNA3.1 (4.0 µg), or miR-742-3p (50 nM) + pcDNA3.1- FOXO1 (4.0 µg), and then treated with hypoxia. Untreated H9c2 cells were used as control. Cell viability, migrated and invaded cell numbers, and cell apoptosis rate were determined by CCK8 assay ( B ), transwell assay ( C , D ), and flow cytometry ( E ). F WB analysis was employed to examine the protein levels of Bcl-2, Bax and Cleaved-caspase 3. All experiments were repeated three times. ** P < 0.01, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s11658-022-00330-y'>35346026</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-40364_2022_407_fig4_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;MiR-148a-3p promotes proliferation, while inhibits apoptosis in A549 and HCC827 cells. A qPCR assay confirming the transfection efficiency of the miR-148a-3p mimic and miR-148a-3p inhibitor in A549 and HCC827 cells. B Viability in A549 and HCC827 cells transfected with miR-NC, miR-148a-3p mimic, miR-148a-3p inhibitor NC, or miR-148a-3p inhibitor. C Edu assay of cell proliferation in A549 and HCC827 cells transfected with miR-NC, miR-148a-3p mimic, miR-148a-3p inhibitor NC, or miR-148a-3p inhibitor, respectively. D-E Cell apoptosis rates of A549 and HCC827 cells with the indicated transfection were determined with FSC assay. Data are presented as mean ± standard deviation. The experiments were repeated three times. F-G Protein levels of Bax, Bcl-2 and Cytochrome in A549 and HCC827 cells with the indicated transfection were determined by western blot. Data are presented as mean ± standard deviation. The experiments were repeated three times. * P < 0.05; ** P < 0.01; *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40364-022-00407-y'>35974419</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12276_2022_735_fig2_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Knockdown of IGF2BP3 significantly inhibits AML progression in vitro. a The protein expression level of IGF2BP3 in various hematologic tumor cell lines was measured by western blotting. b The knockdown efficiency of IGF2BP3 shRNAs (shIGF2BP3#1 and shIGF2BP3#2) delivered via lentiviral vectors in HL-60 and KG-1 cell lines was confirmed by western blotting. GAPDH was used as the internal reference. c Cell proliferation was measured by a CCK-8 assay at different time points (0, 24, 48, 72, and 96 h) in HL-60 and KG-1 cells after shRNA transduction. d The transduction efficiency after puromycin selection was evaluated by GFP fluorescence imaging in both cell lines. e Flow cytometry (representative images are presented) was used to confirm the induction of apoptosis by IGF2BP3 knockdown. f Western blotting was used to explore apoptosis-related protein levels. The levels of cleaved caspase-3 and Bax were increased but the level of Bcl-2 was decreased under shIGF2BP3 treatment compared with control treatment. g Flow cytometry (representative images are presented) was used to analyze the cell cycle distribution. * P < 0.05; ** P < 0.01; *** P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs12276-022-00735-x'>35217832</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-40364_2022_407_fig9_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;Circ_0009043 inhibits tumor growth via targeting the miR-148a-3p / DNAJB4 pathway in vivo. A, C Tumors formed 6 weeks post-injection in BALB/C nude mice. Tumors in the CTRL, OE-CTRL, OE-Circ, shCTRL and shCirc groups were isolated from mice at the endpoint of experiments. B, D Tumor growth was assessed by tumor volume measurement over time in the 5 afore mentioned groups (mean ± SD; n = 5). ** P < 0.01. Mice were anesthetized and sacrificed at experimental endpoints. Tumors were subsequently dissected. E, F Circ_0009043 and miR-148a-3p mRNA expression in tumors from CTRL, OE-CTRL, OE-Circ, shCTRL and shCirc groups, respectively; n = 5. G DNAJB4 protein expression in tumors from CTRL, OE-CTRL, OE-Circ, shCTRL and shCirc groups; n = 5. H Representative images of DNAJB4 IHC in CTRL, OE-CTRL, OE-Circ, shCTRL and shCirc groups, respectively. (× 200, scale bars, 100 µm). I-J TUNEL staining assay was applied to compare the cell apoptosis of CTRL, OE-CTRL, OE-Circ, shCTRL and shCirc groups in tumors (scale bar, 100 μm). K Expression level of Bax, Bcl-2, and Cytochrome C in the cells of the 5 groups (CTRL, OE-CTRL, OE-Circ, shCTRL and shCirc groups) were determined by western blotting. ** P < 0.01, *** P < 0.001, ### P < 0.001; #### P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40364-022-00407-y'>35974419</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1013-1-12872_2025_4762_fig4_html.pngAnti-Apoptosis regulator BAX Bax Antibody Picoband&reg;AC attenuated MIRI by inhibiting apoptosis, and regulting the expressions of Bcl-2, Bax and caspase-3 in myocardial tissue. ( A ) Representative TUNEL assay sections of myocardial tissue in each group. Magnification×200, scale bar, 100 μm. ( B ) The relative fluorescence ratio of TUNEL in each group was quantified using Image J. ( C ) Representative immunohistochemistry sections of caspase-3 expression in myocardial tissue of each group. Magnification×200, scale bar:100 μm; Magnification×400, scale bar:50 μm. ( D ) The expression of caspase-3 in each group was illustrated by statistical histograms. ( E ) The protein expressions of Bcl-2 and Bax in myocardial tissue were presented by representative Western blot bands. ( F and G ) The protein expressions of Bcl-2 and Bax in each group were illustrated by statistical histograms. Data was expressed as mean ± standard deviation ( n = 5). * P < 0.05; ** P < 0.01; *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12872-025-04762-0'>40287651</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bdnf-antibody-pa1014-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1014-1_1-WB-anti-bdnf-antibody.jpgAnti-BDNF Antibody Picoband&reg;Anti-BDNF antibody&#44; PA1014&#44; Western blotting<br>All lanes: Anti BDNF (PA1014) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Lane 3: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 28KD<br>Observed bind size: 37KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calretinin-antibody-pa1015-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1015-1-WB-anti-calretinin-antibody.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg; Western blot analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calretinin/CALB2 antigen affinity purified polyclonal antibody (Catalog # PA1015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calretinin/CALB2 at approximately 29 kDa. The expected band size for Calretinin/CALB2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1015-2-IHC-anti-calretinin-antibody.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg; IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). <br> Calretinin/CALB2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1015-calb2-primary-antibodies-ihc-testing-3.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg; IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). <br> Calretinin/CALB2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1015-calb2-primary-antibodies-ihc-testing-4.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg; IHC analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). <br> Calretinin/CALB2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1015-calb2-primary-antibodies-if-testing-5.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg; IF analysis of Calretinin/CALB2 using anti-Calretinin/CALB2 antibody (PA1015). <br> Calretinin/CALB2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Calretinin/CALB2 Antibody (PA1015) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr5-antibody-pa1016-boster.html2026-03-24T05:03:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1016-2_1.jpgAnti-C-C chemokine receptor type 5 CCR5 Antibody Picoband&reg;Anti-CCR5 antibody&#44; PA1016&#44; Western blotting<br>All lanes: Anti CCR5 (PA1016) at 0.5ug/ml<br>WB: Mouse Liver Tissue Lysate at 50ug<br>Predicted bind size: 40KD<br>Observed bind size: 40KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1016-1_1.jpgAnti-C-C chemokine receptor type 5 CCR5 Antibody Picoband&reg;Anti-CCR5 antibody&#44; PA1016&#44; Western blotting<br>Lane 1: JURKAT Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr5-antibody-pa1016-1-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1016-1-1-WB-anti-ccr5-antibody.jpgAnti-C-C chemokine receptor type 5 CCR5 Antibody Picoband&reg;Anti-CCR5 antibody&#44; PA1016-1&#44; Western blotting<br>Lane 1: COLO320 Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: SMMC Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr5-antibody-pa1016-2-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1016-2-1-WB-anti-ccr5-antibody.jpgAnti-C-C chemokine receptor type 5 CCR5 Antibody Picoband&reg;Anti-CCR5 antibody&#44; PA1016-2&#44; Western blotting<br>Lane 1: Mouse Lung Tissue Lysate<br>Lane 2: Mouse Intestine Tissue Lysate<br>Lane 3: Mouse Kidney Tissue Lysate <br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd22-antibody-pa1018-1-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1018-1-cd22-primary-antibodies-wb-testing-1.jpgAnti-B-cell receptor CD22 CD22 Antibody Picoband&reg; Western blot analysis of CD22 using anti-CD22 antibody (PA1018-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Ramos whole cell lysates, <br> Lane 2: human Daudi whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD22 antigen affinity purified polyclonal antibody (Catalog # PA1018-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD22 at approximately 130 kDa. The expected band size for CD22 is at 95,130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1018-1-2-IHC-anti-cd22-siglec-2-antibody.jpgAnti-B-cell receptor CD22 CD22 Antibody Picoband&reg;Anti-CD22 antibody&#44; PA1018-1&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd40-antibody-pa1019-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1019-1-WB-anti-cd40-tnfrsf5-antibody.jpgAnti-CD40 Antibody Picoband&reg;Anti-CD40 antibody&#44; PA1019&#44; Western blotting<br>Lane 1: Recombinant Mouse CD 40 Protein 10ng<br>Lane 2: Recombinant Mouse CD 40 Protein 5ng<br>Lane 3: Recombinant Mouse CD 40 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd40-antibody-pa1019-1-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1019-1-cd40-primary-antibodies-wb-testing-1.jpgAnti-CD40 Antibody Picoband&reg; Western blot analysis of CD40 using anti-CD40 antibody (PA1019-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates, <br> Lane 2: human Raji whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40 antigen affinity purified polyclonal antibody (Catalog # PA1019-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40 at approximately 40 kDa. The expected band size for CD40 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1019-1-cd40-primary-antibodies-fcm-testing-2_2.jpgAnti-CD40 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-CD40 antibody (PA1019-1). <br>Overlay histogram showing THP-1 cells stained with PA1019-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40 Antibody (PA1019-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd40l-antibody-pa1020-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1020-1_1-WB-anti-scd40l-antibody.jpgAnti-CD40L/CD40LG Antibody Picoband&reg; Western blot analysis of sCD40L using anti-sCD40L antibody (PA1020). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: MCF-7 Whole Cell Lysate&#44;<br> Lane 2: HELA Whole Cell Lysate&#44;<br> Lane 3: JURKAT Whole Cell Lysate&#44;<br> Lane 4: HMY2 Whole Cell Lysate&#44;<br> Lane 5: COLO320 Whole Cell Lysate.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-sCD40L antigen affinity purified polyclonal antibody (Catalog # PA1020) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for sCD40L at approximately 29KD. The expected band size for sCD40L is at 29KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1020-2-IHC-anti-scd40l-antibody.jpgAnti-CD40L/CD40LG Antibody Picoband&reg; IHC analysis of sCD40L using anti-sCD40L antibody (PA1020).<br> sCD40L was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-sCD40L Antibody (PA1020) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1020-3-IHC-anti-scd40l-antibody.jpgAnti-CD40L/CD40LG Antibody Picoband&reg; IHC analysis of sCD40L using anti-sCD40L antibody (PA1020).<br> sCD40L was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-sCD40L Antibody (PA1020) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd44-antigen-cd44-antibody-pa1021-2-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1021-2-fphar-15-1216199-g004.jpgAnti-CD44 antigen CD44 Antibody Picoband&reg;Representative pictures of caspase-3, Bax, Bcl-2, Ki67, VEGFA, VEGFR-2, MDA, CD24, CD44, ALDH1A1, EpCam, H3K4m3, H3K9m3, H4K20m3, and H4K16ac expressions gained from rat BC samples. For analysis, polyclonal caspase-3 antibody (Bioss, Woburn, USA), polyclonal Bax and Bcl-2 antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA), monoclonal Ki67 antibody (Dako, Glostrup, Denmark), monoclonal VEGFA and VEGFR-2 antibodies (Santa Cruz Biotechnology, Paso Robles, CA, USA), polyclonal CD24 antibody (GeneTex, Irvine, CA, USA), polyclonal CD44 antibody (Boster, Pleasanton, CA, USA), polyclonal ALDH1A1 antibody (ThermoFisher, Rockford, IL, USA), polyclonal MDA, EpCAM, H3K4m, H3K9m3, and H4K20m3 antibodies (Abcam, Cambridge, MA, USA), and monoclonal H4K16ac antibody (Abcam, Cambridge, MA, USA) were applied. The final microscope magnification of ×400 was used.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1216199/full'>38464730</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1021-2-cd44-primary-antibodies-fcm-testing-5.jpgAnti-CD44 antigen CD44 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-CD44 antibody (PA1021-2). <br>Overlay histogram showing HL-60 cells stained with PA1021-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD44 Antibody (PA1021-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1021-2-cd44-primary-antibodies-wb-testing-1.jpgAnti-CD44 antigen CD44 Antibody Picoband&reg; Western blot analysis of CD44 using anti-CD44 antibody (PA1021-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: rat PC-12 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (Catalog # PA1021-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 82 kDa. The expected band size for CD44 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1021-2-cd44-primary-antibodies-wb-testing-6.pngAnti-CD44 antigen CD44 Antibody Picoband&reg; Western blot analysis of CD44 using anti-CD44 antigen CD44 antibody (PA1021-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw) for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen CD44 antibody (PA1021-2) at 1 ug/ml in 5% milk/BPS/Tw overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at at 1:3,000 in 5% milk/PBS/Tw for 1.5 hour at RT. The signal is developed using a chemiluminescence: West Pico from Thermo Scientific. A specific band was detected for CD44 at approximately 81 kDa. The expected band size for CD44 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1021-2-cd44-primary-antibodies-ihc-testing-2.jpgAnti-CD44 antigen CD44 Antibody Picoband&reg; IHC analysis of CD44 using anti-CD44 antibody (PA1021-2). <br> CD44 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD44 Antibody (PA1021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1021-2-cd44-primary-antibodies-ihc-testing-3.jpgAnti-CD44 antigen CD44 Antibody Picoband&reg; IHC analysis of CD44 using anti-CD44 antibody (PA1021-2). <br> CD44 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD44 Antibody (PA1021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1021-2-cd44-primary-antibodies-ihc-testing-4.jpgAnti-CD44 antigen CD44 Antibody Picoband&reg; IHC analysis of CD44 using anti-CD44 antibody (PA1021-2). <br> CD44 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD44 Antibody (PA1021-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gjb2-antibody-pa1025-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1025-1-WB-anti-gjb2-connexin-26-antibody.jpgAnti-Gap junction beta-2 protein GJB2 Antibody Picoband&reg;Anti-GJB2 antibody&#44; PA1025&#44; Western blotting<br>WB: Rat Liver Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-connexin-43-gja1-antibody-pa1026-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-wb-testing-1.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;Western blot analysis of GJA1 using anti-GJA1 antibody (PA1026). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat H9C2 cells whole cell lysates,<br> Lane 4: mouse heart tissue lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA1 antigen affinity purified polyclonal antibody (PA1026) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GJA1 at approximately 43 kDa. The expected band size for GJA1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-ihc-testing-1.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;IHC analysis of GJA1 using anti-GJA1 antibody (PA1026). <br>GJA1 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-ihc-testing-2.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;IHC analysis of GJA1 using anti-GJA1 antibody (PA1026). <br>GJA1 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-ihc-testing-3_1.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;IHC analysis of GJA1 using anti-GJA1 antibody (PA1026). <br>GJA1 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-ihc-testing-4.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;IHC analysis of GJA1 using anti-GJA1 antibody (PA1026). <br>GJA1 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-ihc-testing-5.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;IHC analysis of GJA1 using anti-GJA1 antibody (PA1026). <br>GJA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-ihc-testing-6_1.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;IHC analysis of GJA1 using anti-GJA1 antibody (PA1026). <br>GJA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-gja1-primary-antibodies-if-testing-1.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg;IF analysis of GJA1 using anti-GJA1 antibody (PA1026). <br> GJA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-12872_2020_1385_fig5_html.pngAnti-Connexin 43/GJA1 Antibody Picoband&reg;Expression levels of the p-Cx43, t-Cx43, G-CSFR, p-JAK2, t-JAK2, p-STAT3, and t-STAT3 proteins in rabbit myocardium from each group. The expression levels of each protein were determined using western blot. Left: representative immunoblots showing the expression of the various proteins in each group. Right: quantification of the protein bands. Data are shown as means ± standard deviations ( n = 10/group, except n = 9 in the AG490 group). * P < 0.05, ** P < 0.01 vs. Sham group; # P < 0.05, ## P < 0.01 vs. CME group; && P < 0.01 vs. G-CSF group (one-way ANOVA with LSD post-hoc test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12872-020-01385-5'>32066388</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-12872_2020_1385_fig4_html.pngAnti-Connexin 43/GJA1 Antibody Picoband&reg;Expressions of p-Cx43, t-Cx43, and G-CSFR proteins in rabbit myocardium from each group. The expression levels of each protein were determined using immunohistochemistry. Positive staining is shown in brown; the nuclei are stained blue-black. a p-Cx43. b t-Cx43. c G-CSFR. Quantification of expression was performed under high magnification (400×). The black arrows indicate representative enriched areas, but not all areas are indicated by arrows in the images. Data are shown as means ± standard deviations ( n = 10/group, except n = 9 in the AG490 group). * P < 0.05, ** P < 0.01 vs. Sham group; # P < 0.05, ## P < 0.01 vs. CME group (one-way ANOVA with LSD post-hoc test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12872-020-01385-5'>32066388</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-connexin-43-gja1-antibody-pa1026-1-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1026-1-1-IHC-anti-connexin-43-gja1-antibody.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).<br> Connexin 43/GJA1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1026-1-2-IHC-anti-connexin-43-gja1-antibody.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).<br> Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1026-1-3-IHC-anti-connexin-43-gja1-antibody.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).<br> Connexin 43/GJA1 was detected in frozen section of rat cardiac muscle tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-1-4_1.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates&#44; <br> Lane 2: rat brain tissue lysates&#44; <br> Lane 3: rat hear tissue lysates&#44; <br> Lane 4: mouse brain tissue lysates&#44;<br> Lane 5: mouse heart tissue lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody (Catalog # PA1026-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 43/GJA1 at approximately 43KD. The expected band size for Connexin 43/GJA1 is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-1-5.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).<br> Connexin 43/GJA1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-1-6.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).<br> Connexin 43/GJA1 was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-1-7.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).<br> Connexin 43/GJA1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1026-1-8.jpgAnti-Connexin 43/GJA1 Antibody Picoband&reg; IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026-1).<br> Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-reactive-protein-antibody-pa1028-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1028-1-WB-anti-crp-c-reactive-protein-antibody.jpgAnti-C Reactive Protein/CRP Antibody Picoband&reg;Anti-C Reactive Protein antibody&#44; PA1028&#44; Western blotting<br>Lane 1: SMMC Cell Lysate<br>Lane 2: HT1080 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcr2-antibody-pa1029-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1029-cxcr2-primary-antibodies-wb-testing-1.jpgAnti-CXCR2 Antibody Picoband&reg; Western blot analysis of CXCR2 using anti-CXCR2 antibody (PA1029). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: human COLO320 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR2 antigen affinity purified polyclonal antibody (Catalog # PA1029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR2 at approximately 45 kDa. The expected band size for CXCR2 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dut-antibody-pa1030-1-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1030-1-dut-primary-antibodies-wb-testing-1.jpgAnti-DUT Antibody Picoband&reg;Western blot analysis of DUT using anti-DUT antibody (PA1030-1). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human SH-SY5Y whole cell lysates,<br> Lane 4: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUT antigen affinity purified polyclonal antibody (PA1030-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DUT at approximately 18 kDa. The expected band size for DUT is at 18, 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1030-1-dut-primary-antibodies-ihc-testing-1.jpgAnti-DUT Antibody Picoband&reg;IHC analysis of DUT using anti-DUT antibody (PA1030-1). <br>DUT was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DUT Antibody (PA1030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1030-1-dut-primary-antibodies-ihc-testing-2.jpgAnti-DUT Antibody Picoband&reg;IHC analysis of DUT using anti-DUT antibody (PA1030-1). <br>DUT was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DUT Antibody (PA1030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1030-1-dut-primary-antibodies-ihc-testing-3.jpgAnti-DUT Antibody Picoband&reg;IHC analysis of DUT using anti-DUT antibody (PA1030-1). <br>DUT was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DUT Antibody (PA1030-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1030-1-dut-primary-antibodies-if-testing-1.jpgAnti-DUT Antibody Picoband&reg;IF analysis of DUT using anti-DUT antibody (PA1030-1) and anti-Alpha Tubulin antibody (M03989-3). <br>DUT was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DUT Antibody (PA1030-1) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf4-antibody-pa1033-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1033-1-WB-anti-fgf4-antibody.jpgAnti-Fibroblast growth factor 4 FGF4 Antibody Picoband&reg;Anti-FGF4 antibody&#44; PA1033&#44; Western blotting<br>WB: HELA Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gap43-antibody-pa1037-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1037-1_1-WB-anti-gap43-antibody.jpgAnti-Neuromodulin GAP43 Antibody Picoband&reg;Anti-GAP43 antibody&#44; PA1037&#44; Western blotting<br>All lanes: Anti GAP43 (PA1037) at 0.5ug/ml<br>Lane 1: U87 Whole Cell Lysate at 40ug<br>Lane 2: Rat Brain Tissue Lysate at 50ug<br>Lane 3: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 43KD<br>Observed bind size: 43KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1037-2_1-IHC-anti-gap43-antibody.jpgAnti-Neuromodulin GAP43 Antibody Picoband&reg;Anti-GAP43 antibody&#44; PA1037&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1037-4-IHC-anti-gap43-antibody.jpgAnti-Neuromodulin GAP43 Antibody Picoband&reg;Anti-GAP43 antibody&#44; PA1037&#44; IHC(P)<br>IHC(P): Human Meningeoma Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1037-3-IHC-anti-gap43-antibody.jpgAnti-Neuromodulin GAP43 Antibody Picoband&reg;Anti-GAP43 antibody&#44; PA1037&#44; IHC(P)<br>IHC(P): Human Glioma Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gst3-gst-pi-antibody-pa1040-boster.html2026-03-24T05:03:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1040-gstp1-primary-antibodies-wb-testing-1.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; Western blot analysis of GSTP1 using anti-GSTP1 antibody (PA1040). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: rat lung tissue lysates,<br> Lane 5: mouse lung tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTP1 antigen affinity purified polyclonal antibody (Catalog # PA1040) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTP1 at approximately 23 kDa. The expected band size for GSTP1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1040-gstp1-primary-antibodies-ihc-testing-2.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; IHC analysis of GSTP1 using anti-GSTP1 antibody (PA1040). <br> GSTP1 was detected in a paraffin-embedded section of human highly differentiated adenocarcinoma of the colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1040-gstp1-primary-antibodies-ihc-testing-3.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; IHC analysis of GSTP1 using anti-GSTP1 antibody (PA1040). <br> GSTP1 was detected in a paraffin-embedded section of human moderately differentiated adenocarcinoma of the cervical canal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1040-gstp1-primary-antibodies-ihc-testing-4.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; IHC analysis of GSTP1 using anti-GSTP1 antibody (PA1040). <br> GSTP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GSTP1 Antibody (PA1040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmgb4-antibody-pa1042-boster.html2026-03-24T05:03:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1042-1-WB-anti-hmgb4-antibody.jpgAnti-High mobility group protein B4 HMGB4 Antibody Picoband&reg;Anti-HMGB4 antibody&#44; PA1042&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Testis Tissue Lysate<br>Lane 3: JURKAT Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-alpha-1-antibody-pa1045-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1045-1-itga1-primary-antibodies-wb-testing-1.jpgAnti-Integrin alpha 1/ITGA1 Antibody Picoband&reg; Western blot analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Alpha 1/ITGA1 antigen affinity purified polyclonal antibody (Catalog # PA1045-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Alpha 1/ITGA1 at approximately 190-200 kDa. The expected band size for Integrin Alpha 1/ITGA1 is at 190-200 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1045-1-itga1-primary-antibodies-ihc-testing-2.jpgAnti-Integrin alpha 1/ITGA1 Antibody Picoband&reg; IHC analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). <br> Integrin Alpha 1/ITGA1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Integrin Alpha 1/ITGA1 Antibody (PA1045-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1045-1-itga1-primary-antibodies-ihc-testing-3.jpgAnti-Integrin alpha 1/ITGA1 Antibody Picoband&reg; IHC analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). <br> Integrin Alpha 1/ITGA1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Integrin Alpha 1/ITGA1 Antibody (PA1045-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1045-1-itga1-primary-antibodies-ihc-testing-4.jpgAnti-Integrin alpha 1/ITGA1 Antibody Picoband&reg; IHC analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). <br> Integrin Alpha 1/ITGA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Integrin Alpha 1/ITGA1 Antibody (PA1045-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1045-1-itga1-primary-antibodies-ihc-testing-5.jpgAnti-Integrin alpha 1/ITGA1 Antibody Picoband&reg; IHC analysis of Integrin Alpha 1/ITGA1 using anti-Integrin Alpha 1/ITGA1 antibody (PA1045-1). <br> Integrin Alpha 1/ITGA1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Integrin Alpha 1/ITGA1 Antibody (PA1045-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kcnn4-antibody-pa1047-1-boster.html2026-03-24T05:03:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1047-1-kcnn4-primary-antibodies-wb-testing-1.jpgAnti-KCNN4 Antibody Picoband&reg; Western blot analysis of KCNN4 using anti-KCNN4 antibody (PA1047-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNN4 antigen affinity purified polyclonal antibody (Catalog # PA1047-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNN4 at approximately 48 kDa. The expected band size for KCNN4 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lamin-b1-antibody-pa1048-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-wb-testing-1_1.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human SH-SY5Y whole cell lysates, <br> Lane 4: rat spleen tissue lysates, <br> Lane 5: rat PC-12 whole cell lysates, <br> Lane 6: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (Catalog # PA1048) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 70-72 kDa. The expected band size for Lamin B1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-ihc-testing-1.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-ihc-testing-2.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-ihc-testing-3.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-ihc-testing-4.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-ihc-testing-5.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-if-testing-1_1.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IF analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-if-testing-2_1.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IF analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-if-testing-3.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IF analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-if-testing-4.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;IF analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048). <br> Lamin B1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Lamin B1 Antibody (PA1048) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1048-lmnb1-primary-antibodies-ip-testing-1.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;Immunoprecipitating (IP) Lamin B1 in Hela whole cell lysate.<br> Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PA1048); <br> Lane 1: Hela whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Lamin B1 antibody in Hela whole cell lysate;<br> Lane 3: anti-Lamin B1 antibody (2μg) + Hela whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (PA1048) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Lamin B1 at approximately 70-72 kDa. The expected band size for Lamin B1 is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mapk1-3-antibody-pa1049-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1049-mapk1-3-primary-antibodies-wb-testing-1.jpgAnti-MAPK1/3 Antibody Picoband&reg; Western blot analysis of MAPK1/3 using anti-MAPK1/3 antibody (PA1049). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: human U2OS whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat C6 whole cell lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse Neuro-2a whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK1/3 antigen affinity purified polyclonal antibody (PA1049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAPK1/3 at approximately 38, 42 kDa. The expected band size for MAPK1/3 is at 36, 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1049-mapk1-3-primary-antibodies-if-testing-2.jpgAnti-MAPK1/3 Antibody Picoband&reg; IF analysis of MAPK1/3 using anti-MAPK1/3 antibody (PA1049) and anti-Tubulin Alpha antibody (M03989-3).<br> MAPK1/3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MAPK1/3 Antibody (PA1049) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1049-mapk1-3-primary-antibodies-fcm-testing-3.jpgAnti-MAPK1/3 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-MAPK1/3 antibody (PA1049). <br> Overlay histogram showing Hela cells stained with PA1049 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAPK1/3 Antibody (PA1049, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myelin-basic-protein-antibody-pa1050-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-wb-testing-1.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;Western blot analysis of MBP using anti-MBP antibody (PA1050). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MBP antigen affinity purified polyclonal antibody (PA1050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MBP at approximately 15-22 kDa. The expected band size for MBP is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-1.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-nrr-14-1765-g003.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;GFAP, NF200 and MBP protein expression in nerve grafts 8 weeks after surgery. (A) Western blot assay for GFAP, NF200 and MBP in nerve grafts from over-TrkA, vector, TrkA-shRNA and control groups. (B) The bands were quantified by densitometry and normalized to β-actin. Data are expressed as the mean ± SEM ( n = 5 per group; one-way analysis of variance followed by Student-Newman-Keuls post hoc test). * P < 0.05, ** P < 0.01, *** P < 0.001, vs . vector and control groups. NF200: Neurofilament 200; GFAP: glial fibrillary acidic protein; MBP: myelin basic protein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6585565/&orig_host=www.ncbi.nlm.nih.gov'>31169194</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-fneur-09-00282-g007.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;Thrombin receptor antagonist peptide promoted remyelination and neural functional recovery after subarachnoid hemorrhage (SAH). (A,B) Representative images of transmission electron microscopy and myelin basic protein (MBP) expression at 3 days after SAH are shown. Scale bar = 20 µm. (C) Respective myelin g -ratios, n = 125 for each group. (D) Respective modified neurological severity scale (mNSS) scores in each group at 1, 3, 5, 7, 9, 12, and 14 days after SAH, n = 10 for each group. The data are presented as the mean ± SEM. * P < 0.05 versus SAH + vehicle group, *** P < 0.001 versus SAH + vehicle group. n.s. indicates no significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2018.00282/full'>29922213</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-12993_2025_282_fig3_html.pngAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;Post-treatment immunostaining intensity of MBP protein with immunohistochemistry in medial PFC. A MBP protein in the control group; B . MBP protein in the CUS group; C . MBP protein in the CUS + FLU group; D . MBP protein in the CUS + EEgroup; E . MBP protein in the CUS + FLU + EE group. F . Immunohistochemical Quantification of MBP Protein in medial PFC. All scale bars represent 50 μm; “→”: positive staining in representative medial PFC. Significant differences were revealed with * P < 0.05, ** P < 0.01, n = 6 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12993-025-00282-1'>40500721</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-12993_2025_282_fig5_html.pngAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;Post-treatment protein expression levels of MBP, CNP, and IL-1βwith western-blot in PFC. A Western blot bands of MBP, CNP, and IL-1βin PFC; B . Quantitative Analysis of MBP Protein by western blot; C . Quantitative Analysis of IL-1β Protein by western blot; D . Quantitative Analysis of CNP Protein by western blot. Significant differences were revealed with * P < 0.05, ** P < 0.01, *** P < 0.001, n = 6 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12993-025-00282-1'>40500721</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-12993_2025_282_fig6_html.pngAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;Post-treatment mRNA expression levels of MBP, CNP, and IL-1β in PFC. A Post-treatment mRNA level of MBP with qRT-PCR; B . Post-treatment mRNA level of CNP with qRT-PCR; C . Post-treatment mRNA level of IL-1β with qRT-PCR; Significant differences were revealed with * P < 0.05, ** P < 0.01, *** P < 0.001, n = 6 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12993-025-00282-1'>40500721</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050_jcmm-29-e70744-g007.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;BBR efficacy in reducing glial activation and the secretion of inflammatory cytokines in the hippocampus following anaesthesia and surgical interventions. (A) Utilises immunofluorescence staining to measure expression levels of GFAP across hippocampal regions CA1, CA3 and dentate gyrus (DG). Magnification: 400×. Scale bar = 50 μm. (B) Quantification of the GFAP‐positive fluorescence intensity in CA1, CA3 and DG areas. (C, D) Display the results of western blot analyses detailing the relative concentrations of TNF‐α and IL‐1β proteins in the hippocampus. Statistical values are expressed as mean ± SD, n = 3, significance indicated by * p < 0.05, ** p < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12308226/'>40735856</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-2.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-3.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-4.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-5.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-6.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-7.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-ihc-testing-11.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IHC analysis of Myelin basic protein/MBP using anti-Myelin basic protein/MBP antibody (PA1050). <br>Myelin basic protein/MBP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myelin basic protein/MBP Antibody (PA1050) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-if-testing-1.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IF analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-if-testing-2.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IF analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-if-testing-3.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IF analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-if-testing-4.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IF analysis of MBP using anti-MBP antibody (PA1050). <br> MBP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. HRP Conjugated Goat Anti-Rabbit IgG (BA1054) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Tyramide signal amplification was performed using TSA 570 reagent at 1:200 dilution at room temperature for 10 minutes. Fluorescence signals were visualized using a fluorescence microscope with filter sets appropriate for TSA 570 and DAPI.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-if-testing-5.jpgAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IF analysis of MBP using anti-MBP antibody (PA1050).<br> MBP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 10 μg/mL rabbit anti-MBP Antibody (PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1050-mbp-primary-antibodies-if-testing-6.pngAnti-Myelin Basic Protein/MBP Antibody Picoband&reg;IF analysis of MBP using anti-MBP antibody ( PA1050). <br> ACAD9 was detected in a Frozen section of mouse spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 3% BSA. The tissue section was then incubated with 1:100 rabbit anti-MBP Antibody (PA1050) overnight at 4°C. CY3-conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a laser focol. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mif-antibody-pa1052-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1052-mif-primary-antibodies-wb-testing-1_1_1.jpgAnti-MIF Antibody Picoband&reg; Western blot analysis of MIF using anti-MIF antibody (PA1052). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: rat NRK whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse kidney tissue lysates,<br> Lane 7: mouse HBZY-1 whole cell lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (Catalog # PA1052) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1052-mif-primary-antibodies-ihc-testing-2.jpgAnti-MIF Antibody Picoband&reg; IHC analysis of MIF using anti-MIF antibody (PA1052). <br> MIF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIF Antibody (PA1052) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1052-mif-primary-antibodies-ihc-testing-3.jpgAnti-MIF Antibody Picoband&reg; IHC analysis of MIF using anti-MIF antibody (PA1052). <br> MIF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIF Antibody (PA1052) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1052-mif-primary-antibodies-fcm-testing-4.jpgAnti-MIF Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-MIF antibody (PA1052). <br> Overlay histogram showing K562 cells stained with PA1052 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIF Antibody (PA1052, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-morg1-antibody-pa1053-boster.html2026-03-24T05:03:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1053-1-WB-anti-morg1-antibody.jpgAnti-Morg1/WDR83 Antibody Picoband&reg;Anti-Morg1 antibody&#44; PA1053&#44; Western blotting<br>WB: Rat Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1053-2-IHC-anti-morg1-antibody.jpgAnti-Morg1/WDR83 Antibody Picoband&reg;Anti-Morg1 antibody&#44; PA1053&#44; IHC(P)<br>IHC(P): Rat Lung Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mpo-antibody-pa1054-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-wb-testing-1.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; Western blot analysis of MPO using anti-MPO antibody (PA1054). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HL-60 whole cell lysates, <br> Lane 2: rat thymus tissue lysates, <br> Lane 3: mouse spleen tissue lysates, <br> Lane 4: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # PA1054) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 60 kDa. The expected band size for MPO is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-ihc-testing-2.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PA1054). <br> MPO was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody (PA1054) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41598_2017_851_fig3_html.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Effects of NMN on microglia activation and neutrophil infiltration in cICH model. ( A ) Representative images and quantitative analysis of Iba-1 (microglia marker) immunohistochemistry staining at 24 hours post cICH. ** P < 0.01, n = 5 per group. ( B ) Representative images and quantitative analysis of MPO-1 (neutrophil marker) immunohistochemistry staining at 24 hours post cICH. ** P < 0.01, n = 5 per group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00851-z'>28386082</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-ihc-testing-3.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PA1054). <br> MPO was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody (PA1054) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41467_2024_52812_fig6_html.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;RC NP-mediated modulation of immune cell dynamics post-MI. a Representative IHC images and quantitative analysis of apoptotic (TUNEL + , green) neutrophils (MPO + , pink) in infarcted rat hearts 1 day post-MI ( n = 3 for sham, 6 for other groups). Scale bars, 25 µm. b Representative IHC images and quantitative analysis of neutrophils (MPO + , red) that have undergone NETosis (CitH3 + , green) in infarcted rat hearts 3 days post-MI ( n = 3 for sham, 6 for other groups). Scale bars, 25 µm. c Representative IHC images and quantitative analysis of iNOS + (green) macrophages (CD68 + , red) in infarcted hearts 3 days post-MI ( n = 3 for sham, 6 for other groups). Scale bars, 25 µm. d Representative IHC images and quantitative analysis of CD206 + (green) macrophages (CD68 + , red) in infarcted hearts 3 days post-MI ( n = 3 for sham, 6 for other groups). Scale bars, 25 µm. e CD86 and CD80 expression in macrophages (CD68 + cells) 3 and 5 days after MI as determined by flow cytometry (biological replicates n = 4 for D3/D5 sham, D3 R NP, D3 Free R, D5 C NP, and D5 RC NP, 5 for D3 PBS, D3 C NP, D3 RC NP, D5 R NP, and D5 Free R, and 6 for D5 PBS). f Serum levels of NET-associated chemoattractant protein S100A9 1 and 3 days post-MI as determined by ELISA ( n = 3 biological replicates). g Serum levels of neutrophil granule-derived LL-37 1 day post-MI as determined by ELISA ( n = 3 biological replicates). * p < 0.05 vs PBS; § p < 0.05 vs C NP; ‡ p < 0.05 vs R NP; # p < 0.05 vs Free R; ⊤ p < 0.05 vs RC NP; ⊥ p < 0.05 vs Sham. All data presented as mean ± SD. One-way ANOVA was used for all comparisons. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-52812-6'>39353987</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-ihc-testing-4.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PA1054). <br> MPO was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody (PA1054) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-if-testing-5.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IF analysis of MPO using anti-MPO antibody (PA1054). <br> MPO was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MPO Antibody (PA1054) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41467_2024_54685_fig7_html.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Clonal relationships and developmental trajectory of CD8 + T cells. a The doughnut charts showing the distribution of the clonal status of CD8 + T cells across four groups (5 CBL, 8 NP-BL, 4 CIT, and 8 NP samples) in our dataset. b The index scores of the abundance-based coverage estimator (ACE), Chao1 richness estimator (Chao), inverse Simpson index (Inv.Simpson), inverse Pielou evenness index (Inv.Pielou), and Shannon entropy of CD8 + T cells across four groups (5 CBL, 8 NP-BL, 4 CIT, and 8 NP samples). Box plots show median, quantiles, minimum and maximum. Two-sided Kruskal-Wallis with Dunn’s multiple comparisons test. c Cell counts of CD8 + T cell subsets across four groups (up); The distribution of clone status in CD8 + T cell subsets across four groups (down) (5 CBL, 8 NP-BL, 4 CIT, and 8 NP samples). d Top 100 clonal types relative to GZMK + CD8 + T cells in different tissues, showing from top to bottom: cell counts of GZMK + CD8 + T cell in each clonotype, the cell composition of each clonotype, the total cell distribution and GZMK + CD8 + T cell distribution of each clonotype across different sample types. e Single T cell analysis by RNA sequencing and TCR tracking (STARTRAC) analysis estimating the clonal expansion (STARTRAC-expa) and state transition (STARTRAC-tran) of CD8 + T cell clusters across four groups (5 CBL, 8 NP-BL, 4 CIT, and 8 NP samples). f Pseudotime and developmental trajectory of CD8 + T cells inferred by Monocle 3 on UMAP plot. g Two-dimensional plots displaying expression scores for three typical gene signatures in cells of paths 1 (brown), path 2 (yellow) and path 3 (red), along the Pseudotime, respectively. h Potential antigens imputed from the top 10 clonotypes of GZMK + CD8 + T cells from NPs in TCRmatch. According to the instructions of TCRmatch, score >0.97 were considered as reliable. CBL control blood sample, CD8 + Tpex CD8 + progenitor exhausted cells, CIT control inferior turbinate sample, MAIT mucosal associated invariant T cell, NP nasal polyp, NP-BL blood sample from CRSwNP patient. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54685-1'>39614076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-if-testing-6.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IF analysis of MPO using anti-MPO antibody (PA1054). <br> MPO was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MPO Antibody (PA1054) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41467_2024_54685_fig6_html.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Interaction between GZMK + CD8 + T cells and fibroblasts contributes to neutrophilic inflammation in nasal polyps. a Representative immunofluorescence staining of collagen I (COL1A1, green), CD8 (red), and GZMK (yellow) in NPs. The right image shows a greater magnification of the outlined area. b Spatial distribution analysis of GZMK + CD8 + T and COL1A1 + cells in the same tissue field demonstrated in ( a ) using HALO software. c–d The number of COL1A1 + fibroblasts within a radius of 25 μm from the nuclear center of GZMK + CD8 + T, GZMB + CD8 + T, CD4 + T, or CD19 + B cells in CIT group (left, n = 10 samples) and NP group (right, n = 10 samples) ( c ). Average distance from the indicated cell types to the closest COL1A1 + fibroblasts in CIT group (left, n = 10 samples) and NP group (right, n = 10 samples) ( d ). e DEGs between NP-derived primary fibroblasts (NPDF) treated with and without recombinant human GZMK ( n = 4). Two-sided Wald test (default for DESeq2 r-package) was used for differential expression analysis utilizing standard cutoffs of https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41467_2024_54685_fig5_html.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Spatial proximity between GZMK + CD8 + T cells and fibroblasts revealed by Visium HD. a HE staining of the nasal polyp specimen from a representative CRSwNP patient (HD_NP4) in Visium HD (left). Cellular annotation of each 16 × 16 µm bin in the tissue specimen by deconvolution using the public ScRNA-seq dataset (right). b Spatial distribution of CD8T_GZMK (left) and GZMK expression (right) in the HD_NP4 sample. c Spatial distribution of CD8T_GZMB (left) and GZMB expression (right) in the HD_NP4 sample. d Spatial distribution of epithelial cells (left) and KRT19 expression (right) in the HD_NP4 sample. e Spatial distribution of fibroblasts (left) and FBLN1 expression (right) in the HD_NP4 sample. f Bar plots showing the cell type composition in each sample from control participants and patients with CRSwNP. g Bar plots showing the total counts of each cell type in CIT (left, n = 4 samples) and NP (right, n = 6 samples) groups. h , i , j Proportions of CD8T_GZMK and CD8T_GZMB in CD8 + T cells in nasal tissue samples in the indicated group (4 CIT and 6 NP samples) ( h ). Proportions of the major immune cells (B cells and CD4T cells) detected in the indicated group (4 CIT and 6 NP samples) ( i ). Proportions of the major structural cells (fibroblasts, epithelial cells, and SMCs) detected in the indicated group (4 CIT and 6 NP samples) ( j ). In ( h – j ), Box plots show median, quantiles, minimum and maximum. Two-sided unpaired Wilcoxon test. k Neighborhood enrichment analysis between cell types in spatial coordinates. The “CD8T_GZMK” and “fibroblasts” show a positive enrichment score. l Dot plots showing interactions between chemokine ligands (in structural cells) and receptors (in CD8 + T subsets). P values are computed from one-sided permutation test (default for CellChat r-package). Dot size represents P value. The color represents the communication possibility between CD8 + T subsets and structural cells. CIT control inferior turbinate sample, ECs endothelial cells, ILC innate lymphoid cell, NK natural killer cell, NP nasal polyp, pDCs plasmacytoid dendritic cells, SMCs smooth muscle cells. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54685-1'>39614076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41467_2024_54685_fig3_html.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;GZMK + CD8 + T cells are the primary cellular source of GZMK in nasal polyps. a The UMAP depicting the expression pattern of GZMK in CD45 + lymphocytes in 25 samples (5 CBL, 8 NP-BL, 4 CIT, and 8 NP samples) of our dataset. b The doughnut chart showing the composition of GZMK expressing cells in our dataset. c The UMAP depicting the expression pattern of GZMK in all CD8 + T cells from our dataset. d Violin plot showing the expression levels of GZMK in the indicated cell types. e The doughnut chart showing the composition of GZMK expressing CD8 + T cells in different sample groups in our dataset. Representative flow cytometry plots ( f ) and cumulative data ( g ) showing GZMK and GZMB expression among CD8 + T cells from indicated groups (22 CBL, 27 NP-BL, 14 CIT, and 30 NP samples). Data are presented as median with interquartile ranges; Two-sided Kruskal-Wallis with Dunn’s multiple comparisons test. h Dot plot displaying the expression of selected cell surface markers and transcription factors in GZMK + CD8 + T and GZMB + CD8 + T cells from NPs. i , j Flow cytometry showing mean fluorescence intensity (MFI) of CXCR4, PD-1, EOMES, and T-bet protein levels in GZMK + CD8 + T and GZMB + CD8 + T cells from NPs ( n = 17 samples). Two-sided paired t test. k , l Representative immunofluorescence staining of GZMK (green) and CD8 (red) colocalization in control inferior turbinate tissues (CIT, left) and NP samples (NP, right) ( k ). Quantified results of GZMK and CD8 double-positive cells in the indicated group (17 CIT and 57 NP samples) ( l ). Scale bar: 40 μm. HPF high power field. Data are presented as median with interquartile ranges; Two-sided unpaired Wilcoxon test. m , n Representative immunofluorescence staining of GZMB (green) and CD8 (red) colocalization in CIT (left) and NP samples (right) ( m ). Quantified results of GZMB and CD8 double-positive cells in the indicated group (10 CIT and 10 NP samples) ( n ). Scale bar: 40 μm. HPF high power field. Data are presented as median with interquartile ranges; Two-sided unpaired Wilcoxon test. CBL control blood sample, CD8 + Tpex, CD8 + progenitor exhausted cells, CIT control inferior turbinate sample, MAIT mucosal associated invariant T cell, NP nasal polyp, NP-BL blood sample from CRSwNP patient, Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54685-1'>39614076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41467_2024_54685_fig2_html.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;GZMK + CD8 + T cells are preferentially increased in NPs with a distinct transcriptional program. a UMAP plots showing that 81,202 CD8 + T cells from 25 samples (5 CBL, 8 NP-BL, 4 CIT, and 8 NP samples) are separated into 16 clusters (upper left). Clusters are annotated into nine major cell types by canonical markers (upper right) and colored by different sampling locations (lower left and right). b Dot plots showing the scaled expression of selected canonical marker genes in the indicated cell types. c Feature plots and violin plots illustrating expression of naive, effector memory and cytotoxicity curated gene signatures across CD8 + T cell clusters. d Bar plots showing the compositions of major cell types in each sample across different sampling locations in control participants and patients with CRSwNP. e Tissue prevalence of major cell types in the indicated group (13 blood samples (including 5 CBL and 8 NP-BL samples) and 12 nasal tissue samples (including 4 CIT and 8 NP samples)) is estimated by Ro/e score. f Scatter-plot shows differentially expressed genes (DEGs) between GZMK + CD8 + T cells and other CD8 + T cells. Two-sided Wilcoxon rank-sum tests with Bonferroni correction. Genes with https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-41467_2024_54685_fig1_html.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Distinct immune cell compositions between control participants and CRSwNP patients. a Schematic diagram of the study design for ScRNA-seq and spatial transcriptomics. Part of this figure was created by figdraw.com. b Uniform manifold approximation and projection (UMAP) plots showing that 208,757 cells recovered from 25 samples (5 control blood samples, 4 control inferior turbinate samples, 8 blood samples from patients with CRSwNP, and 8 nasal polyp samples) are separated into 25 cell clusters (upper left). Clusters are annotated into eight major immune cell types by canonical markers (upper right) and colored by different sampling locations (lower left and right). c Dot plots showing the scaled expression of selected canonical marker genes in indicated cell types. The dot size represents percentage of cells expressing the genes in each cell type. The color represents the scaled gene expression level. d Pie charts displaying the cellular frequencies of the eight major cell types in blood ( n = 13 samples) (left) and nasal tissue ( n = 12 samples) (right). e , Bar plots showing the compositions of major immune cell types in each sample across different sampling locations in control participants and patients with CRSwNP. f Tissue prevalence of major cell types in the indicated group (13 blood samples (including 5 CBL and 8 NP-BL samples) and 12 nasal tissue samples (including 4 CIT and 8 NP samples)) is estimated by Ro/e score = (observed cell numbers/expected cell numbers). C control, CBL control blood sample, CIT control inferior turbinate sample, FACS fluorescence-activated cell sorting, ILC2 group 2 innate lymphoid cell, NK natural killer cell, NP nasal polyp, NP-BL blood sample from CRSwNP patient, P patient. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-54685-1'>39614076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-medscimonit-25-794-g003.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg;( A ) Brain tissue inflammatory cell infiltration was examined by HE staining (100×). The expression levels of ( B ) MPO and ( C ) IBA-1 were determined by IHC (400×).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6362757/&orig_host=www.ncbi.nlm.nih.gov'>30686819</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-fmicb-11-622354-g002.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg;The infiltration of MPO + neutrophils, and the cellular distribution and relative expression level detection of the TNF and IL-10 in the small intestinal and colonic mucosa at 7 days after the termination of DSS administration. (A) The MPO immunohistochemistry staining of the small intestinal mucosa: (A1) the normal group: few neutrophils were observed in the small intestinal mucosa; (A2) the DSS group: a number of accumulative MPO + neutrophils (brown) infiltrated into the mucosa epithelium; (A3) the DSS + B. subtilis- fermented milk group: only limited neutrophil infiltration could be observed in the small intestinal mucosa. (B) The MPO immunohistochemistry staining of the colonic mucosa: (B1) the normal group: few neutrophils were observed in the colonic mucosa; (B2) the DSS group: colonic epithelium and the glands disappeared, and the ulcer was locally replaced by scars and a number of accumulative MPO + neutrophils (brown) were observed in the scars; (B3) the DSS + B. subtilis -fermented milk group: only limited MPO + neutrophils observed in the colonic mucosa. (C) The TNF immunohistochemistry staining of the small intestinal mucosa: (C1) the normal group: the epithelium was integrated with faint yellow staining, suggesting low expression of TNF; (C2) the DSS group: the villus structure is not integrated, and the epithelial cells showed black brown, suggesting overexpression of TNF; (C3) the DSS + B. subtilis -fermented milk group: the villus and the glands were almost integrated, and the staining of epithelial cells was similar to that of the normal group (C1) , suggesting low expression of TNF. (D) The TNF immunohistochemistry staining of the colonic mucosa: (D1) the normal colonic mucosa: the epithelium was integrated with low TNF expression (faint yellow); ( D2 ) the DSS group: the epithelium structure and the glands were destroyed and replaced by a scar, and there were a number of TNF + inflammatory cells (black brown) in the scar; (D3) the DSS + B. subtilis -fermented milk group: the recovered epithelium showed faint yellow, suggesting low TNF expression. (E) The IL-10 immunohistochemistry staining of the small intestinal mucosa: (E1) the normal small intestinal mucosa: the IL-10 staining dispersed in the villi and the crypts with faint yellow, suggesting low-level expression of IL-10; (E2) the DSS group, the residual epithelium and the crypts were light brown, suggesting mid-level of IL-10 expression; (E3) the DSS + B. subtilis -fermented milk group: the dark brown staining of the regenerative epithelium represented high-level expression of IL-10. (F) The IL-10 immunohistochemistry staining of the colonic mucosa: (F1) the normal group: the IL-10 staining dispersed in the glands with bright yellow, suggesting low-level expression of IL-10; (F2) the DSS group: there were few IL-10 + cells in the scars; (F3) the DSS + B. subtilis -fermented milk group, the dark brown staining of the epithelial cells represented high-level expression of IL-10. (G,H) Western blotting analysis for the expression of MPO, TNF, and IL-10 in the samples containing equivalent ileum and colon. The expression level of MPO, TNF, and IL-10 in the DSS group was significantly higher than that of the normal (control) group. The expression level of MPO and TNF in the DSS + B. subtilis -fermented milk (FM) group was significantly lower than that of the DSS group, while the expression level of IL-10 in the DSS + B. subtilis -fermented milk (FM) group was significantly higher than that of the DSS group ( n = 5, * represents p < 0.05, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-fmicb-11-622354-g008.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Difference in the mean proportions of the major composition of the intestinal flora. (A) Mean proportions of the top 25 genus in the intestinal flora at 7 days after termination of DSS intake and the statistical difference between group 3 (DSS group) and group 5 (DSS + B. subtilis -fermented milk group) ( n = 5). (B) Significantly different taxa as measured by LEfSe analysis (threshold > 3.5).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-ihc-testing-4.pngAnti-Myeloperoxidase/MPO Antibody Picoband&reg;IHC analysis of MPO using anti-MPO antibody (PA1054). <br> MPO was detected in a paraffin-embedded section of mouse dorsal skin (normal group) and burned skin (model group) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 9.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-MPO Antibody (PA1054) overnight at 4°C. Polymer Anti-Rabbit IgG–HRP IHC Kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1054-mpo-primary-antibodies-fcm-testing-7.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-MPO antibody (PA1054). <br>Overlay histogram showing HL-60 cells stained with PA1054 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody (PA1054, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ngf-antibody-pa1056-boster.html2026-03-24T05:03:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1056-1-WB-anti-ngf-ngf-beta-antibody.jpgAnti-Beta-nerve growth factor NGF Antibody Picoband&reg;Anti-NGF antibody&#44; PA1056&#44; Western blotting<br>WB: Rat Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1056-2-WB-anti-ngf-ngf-beta-antibody.jpgAnti-Beta-nerve growth factor NGF Antibody Picoband&reg;Anti-NGF antibody&#44; PA1056&#44; Western blotting<br>Lane 1: Recombinant Human NGFB Protein 10ng<br>Lane 2: Recombinant Human NGFB Protein 5ng<br> https://www.bosterbio.com/products/primary-antibodies/anti-nmdar2a-antibody-pa1058-1-boster.html2026-03-24T05:03:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1058-1-grin2a-primary-antibodies-ihc-testing-3.jpgAnti-NMDAR2A/GRIN2A Antibody Picoband&reg;IHC analysis of NMDAR2A/GRIN2A using anti-NMDAR2A/GRIN2A antibody (PA1058-1). <br>NMDAR2A/GRIN2A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NMDAR2A/GRIN2A Antibody (PA1058-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1058-1-grin2a-primary-antibodies-ihc-testing-4.jpgAnti-NMDAR2A/GRIN2A Antibody Picoband&reg;IHC analysis of NMDAR2A/GRIN2A using anti-NMDAR2A/GRIN2A antibody (PA1058-1). <br>NMDAR2A/GRIN2A was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NMDAR2A/GRIN2A Antibody (PA1058-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1058-1-grin2a-primary-antibodies-wb-testing-1.jpgAnti-NMDAR2A/GRIN2A Antibody Picoband&reg; Western blot analysis of GRIN2A using anti-GRIN2A antibody (PA1058-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIN2A antigen affinity purified polyclonal antibody (Catalog # PA1058-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIN2A at approximately 180 kDa. The expected band size for GRIN2A is at 165 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1058-1-grin2a-primary-antibodies-ihc-testing-2.jpgAnti-NMDAR2A/GRIN2A Antibody Picoband&reg; IHC analysis of GRIN2A using anti-GRIN2A antibody (PA1058-1). <br> GRIN2A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRIN2A Antibody (PA1058-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmdar2b-antibody-pa1059-boster.html2026-03-24T05:03:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1059-1-WB-anti-nmdar2b-antibody.jpgAnti-NMDAR2B/GRIN2B Antibody Picoband&reg;Anti-NMDAR2B antibody&#44; PA1059&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: Mouse Brain Tissue Lysate<br>Lane 4: Mouse Brain Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmdar2b-antibody-pa1059-1-boster.html2026-03-24T05:03:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1059-1-1-WB-anti-nmdar2b-antibody.jpgAnti-NMDAR2B/GRIN2B Antibody Picoband&reg;Anti-NMDAR2B antibody&#44; PA1059-1&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br>Lane 3: U87 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nse-antibody-pa1061-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1061-1-WB-anti-nse-antibody.jpgAnti-NSE/ENO2 Antibody Picoband&reg;Anti-NSE antibody&#44; PA1061&#44; Western blotting<br>WB: Rat Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1061-eno2-primary-antibodies-ihc-testing-3.jpgAnti-NSE/ENO2 Antibody Picoband&reg;IHC analysis of NSE/ENO2 using anti-NSE/ENO2 antibody (PA1061). <br>NSE/ENO2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NSE/ENO2 Antibody (PA1061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1061-13046_2011_article_497_fig2_html.jpgAnti-NSE/ENO2 Antibody Picoband&reg;Macroscopic examination of the CAM and implanted human NCI-H446 cells . The entire experimental process from the implantation of NCI-H446 cells on the CAM and the formation of the transplantation tumor is shown. (A) Irregular window made in the egg shell of a 7-day-old chick embryo. (B) Elimination of the chick embryo in the CAM was observed. (C) The CAM was peeled for the assay. (D) Diagram of the technique for the implantation of NCI-H446 cells onto the CAM. (E) Diagram of the technique for the formation of the transplantation tumor. (F) The transplantation tumor (white mass was pointed by the tip) was formed on the side facing the chick embryo. (G-H) Histological evaluation of the transplanted tumor on the CAM by hematoxylin-eosin staining is shown:(G) The structure of the transplantation tumor and peripheral vessels (50 ×). (H) Pathological appearance of the transplantation tumor (200 ×). (I) Specific analysis was carried out by immunohistochemistry for the expression of NSE. The cellular nucleus was irregular, and positive expression for NSE was found in the intercellular substance or endochylema (400 ×). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-30-77'>21843314</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1061-2.jpgAnti-NSE/ENO2 Antibody Picoband&reg; IHC analysis of NSE using anti-NSE antibody (PA1061). <br> NSE was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NSE Antibody (PA1061) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neurotrophin-3-antibody-pa1062-boster.html2026-04-03T05:00:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1062-1.jpgAnti-Neurotrophin 3/NTF3 Antibody Picoband&reg;Anti-Neurotrophin 3 antibody&#44; PA1062&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: MCF-7 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1062-2.jpgAnti-Neurotrophin 3/NTF3 Antibody Picoband&reg;Anti-Neurotrophin 3 antibody&#44; PA1062&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1062-fncel-15-629356-g002.jpgAnti-Neurotrophin 3/NTF3 Antibody Picoband&reg;Lentivirus-mediated overexpression or interference of NT-3 in BMSCs. (A,C) The protein level of NT-3 in BMSCs transfected by lentivirus was detected by western blot assay. β-actin was used as a loading control. (B,D) The densitometric analysis results were shown. The data were expressed as means ± SD ( n = 3). The results displayed were obtained from at least three independent experiments. *** P < 0.001, versus the vector group or sh-NC group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2021.629356/full'>33642999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1062-fncel-15-629356-g003.jpgAnti-Neurotrophin 3/NTF3 Antibody Picoband&reg;Effect of NT-3 on the differentiation of BMSCs into neurons. (A) The morphology changes of BMSCs before and after neuronal differentiation induction were observed under the microscope. (B–D) The expressions of NSE, NF-200, and class III β-tubulin were assessed by immunofluorescence staining. Scale bars represent 50 μm. The results displayed were obtained from at least three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2021.629356/full'>33642999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1062-fncel-15-629356-g004.jpgAnti-Neurotrophin 3/NTF3 Antibody Picoband&reg;Effect of NT-3 on Wnt/β-catenin signaling pathway in BMSCs. (A) The mRNA expression of NT-3 in BMSCs was assessed by qPCR. (B) The levels of total and nuclear β-catenin in BMSCs were determined by western blot assay. β-actin and Lamin B were used as loading controls. (C,D) The densitometric analysis results were shown. (E) The expression of β-catenin in BMSCs was detected by immunofluorescence staining. Scale bars represent 50 μm. The data were expressed as means ± SD ( n = 3). The results displayed were obtained from at least three independent experiments. *** P < 0.001, vs. the vector group. ## P < 0.01; ### P < 0.001, vs. the sh-NC group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2021.629356/full'>33642999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1062-fncel-15-629356-g005.jpgAnti-Neurotrophin 3/NTF3 Antibody Picoband&reg;Effect of NT-3 genetic modified BMSCs transplantation on the cognitive function and Wnt/β-catenin pathway in rats with AD. (A) The protein levels of NT-3 in brain tissues were assessed by western blot assay. β-actin was used as a loading control. (B) The densitometric analysis results were shown. (C) The cognitive function of the AD rats were determined by MWM. The mean escape latency of each group was shown. (D) The levels of total and nuclear β-catenin in brain tissues were determined by western blot assay. β-actin and Lamin B were used as loading controls. (E,F) The densitometric analysis results were shown. The data were expressed as means ± SD ( n = 4). The results displayed were obtained from at least three independent experiments. * P < 0.05, ** P < 0.01; *** P < 0.001, vs. the PBS group. # P < 0.05; ## P < 0.01; ### P < 0.001, vs. the BMSCs group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2021.629356/full'>33642999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1062-fncel-15-629356-g006.jpgAnti-Neurotrophin 3/NTF3 Antibody Picoband&reg;Effect of NT-3 genetic modified BMSCs transplantation on neurogenesis in brain tissues. The expressions of NSE (A) and NF-200 (B) in the brain tissues were determined by immunofluorescence staining. Scale bars represent 50 μm. The results displayed were obtained from at least three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2021.629356/full'>33642999</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-alpha-antibody-pa1065-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1065-prkca-primary-antibodies-wb-testing-1.jpgAnti-PKC alpha/PRKCA Antibody Picoband&reg; Western blot analysis of PKC alpha using anti-PKC alpha antibody (PA1065). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates, <br> Lane 2: human HEL whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC alpha antigen affinity purified polyclonal antibody (Catalog # PA1065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC alpha at approximately 77 kDa. The expected band size for PKC alpha is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1065-2-IHC-anti-pkc-alpha-antibody.jpgAnti-PKC alpha/PRKCA Antibody Picoband&reg; IHC analysis of PKC alpha using anti-PKC alpha antibody (PA1065). <br> PKC alpha was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PKC alpha Antibody (PA1065) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pmvk-antibody-pa1067-boster.html2026-04-01T05:01:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1067-pmvk-primary-antibodies-ihc-testing-3.jpgAnti-Phosphomevalonate kinase PMVK Antibody Picoband&reg;IHC analysis of PMVK using anti-PMVK antibody (PA1067). <br>PMVK was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PMVK Antibody (PA1067) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1067-pmvk-primary-antibodies-ihc-testing-4.jpgAnti-Phosphomevalonate kinase PMVK Antibody Picoband&reg;IHC analysis of PMVK using anti-PMVK antibody (PA1067). <br>PMVK was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PMVK Antibody (PA1067) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1067-1-wb-anti-pmvk-phosphomevalonate-kinase-antibody.jpgAnti-Phosphomevalonate kinase PMVK Antibody Picoband&reg; Western blot analysis of PMVK using anti-PMVK antibody (PA1067). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat cardiac muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMVK antigen affinity purified polyclonal antibody (PA1067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PMVK at approximately 22 kDa. The expected band size for PMVK is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1067-2-ihc-anti-pmvk-phosphomevalonate-kinase-antibody.jpgAnti-Phosphomevalonate kinase PMVK Antibody Picoband&reg; IHC analysis of PMVK using anti-PMVK antibody (PA1067).<br> PMVK was detected in paraffin-embedded section of rat skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PMVK Antibody (PA1067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pp2a-alpha-antibody-pa1068-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1068-ppp2ca-primary-antibodies-wb-testing-1.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; Western blot analysis of PP2A-alpha using anti-PP2A-alpha antibody (PA1068). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human CACO-2 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse lung tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse brain tissue lysates,<br> Lane 9: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PP2A-alpha antigen affinity purified polyclonal antibody (Catalog # PA1068) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PP2A-alpha at approximately 36 kDa. The expected band size for PP2A-alpha is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1068-2-IHC-anti-pp2a-alpha-antibody.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IHC analysis of PP2A-alpha using anti-PP2A-alpha antibody (PA1068). <br> PP2A-alpha was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PP2A-alpha Antibody (PA1068) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1068-3-IHC-anti-pp2a-alpha-antibody.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IHC analysis of PP2A-alpha using anti-PP2A-alpha antibody (PA1068). <br> PP2A-alpha was detected in a paraffin-embedded section of Rat Kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PP2A-alpha Antibody (PA1068) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rage-antibody-pa1069-boster.html2026-04-01T05:01:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1069-rage-primary-antibodies-wb-testing-1.jpgAnti-RAGE/AGER Antibody Picoband&reg; Western blot analysis of RAGE using anti-RAGE antibody (PA1069). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE antigen affinity purified polyclonal antibody (Catalog # PA1069) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE at approximately 43 kDa. The expected band size for RAGE is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1069-2-IHC-anti-rage-antibody.jpgAnti-RAGE/AGER Antibody Picoband&reg;Anti-RAGE antibody&#44; PA1069&#44; IHC(P)<br>IHC(P): Rat Lung Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-secretogranin-3-antibody-pa1071-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1071-1-WB-anti-secretogranin-3-antibody.jpgAnti-Secretogranin 3/SCG3 Antibody Picoband&reg;Anti-Secretogranin 3 antibody&#44; PA1071&#44; Western blotting<br>WB: HELA Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1071-2-IHC-anti-secretogranin-3-antibody.jpgAnti-Secretogranin 3/SCG3 Antibody Picoband&reg;Anti-Secretogranin 3 antibody&#44; PA1071&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1071-3-IHC-anti-secretogranin-3-antibody.jpgAnti-Secretogranin 3/SCG3 Antibody Picoband&reg;Anti-Secretogranin 3 antibody&#44; PA1071&#44; IHC(P)<br>IHC(P): Human Rectal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1071-4.jpgAnti-Secretogranin 3/SCG3 Antibody Picoband&reg;Western blot analysis of SCG3 using anti-SCG3antibody (PA1071).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Marker 1113,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat kidney tissue lysates, <br> Lane 4: rat PC-12 whole cell lysates.<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse kidney tissue lysates, <br> Lane 7: mouse pancreas tissue lysates,<br> Lane 8: mouse NEURO-2A whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCG3 antigen affinity purified polyclonal antibody (Catalog # PA1071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCG3 at approximately 62-65KD. The expected band size for SCG3 is at 53KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sonic-hedgehog-antibody-pa1072-1-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1072-1-1-WB-anti-sonic-hedgehog-antibody.jpgAnti-Sonic Hedgehog/SHH Antibody Picoband&reg;Anti-Sonic Hedgehog antibody&#44; PA1072-1&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Rat Intestine Tissue Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: A549 Cell Lysate<br>Lane 5: SMMC Cell Lysate<br>Lane 6: MM231 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1072-1-2-IHC-anti-sonic-hedgehog-antibody.jpgAnti-Sonic Hedgehog/SHH Antibody Picoband&reg;Anti-Sonic Hedgehog antibody&#44; PA1072-1&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1072-1-3-IHC-anti-sonic-hedgehog-antibody.jpgAnti-Sonic Hedgehog/SHH Antibody Picoband&reg;Anti-Sonic Hedgehog antibody&#44; PA1072-1&#44; IHC(P)<br>IHC(P): Human Mammary Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat1-antibody-pa1075-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1075-stat1-primary-antibodies-wb-testing-1.jpgAnti-STAT1 Antibody Picoband&reg; Western blot analysis of STAT1 using anti-STAT1 antibody (PA1075). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: rat NRK whole cell lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # PA1075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 87 kDa. The expected band size for STAT1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1075-stat1-primary-antibodies-if-testing-2.jpgAnti-STAT1 Antibody Picoband&reg; IF analysis of STAT1 using anti-STAT1 antibody (PA1075). <br> STAT1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAT1 Antibody (PA1075) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1075-stat1-primary-antibodies-fcm-testing-3.jpgAnti-STAT1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-STAT1 antibody (PA1075). <br> Overlay histogram showing A549 cells stained with PA1075 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (PA1075, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp2-antibody-pa1076-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1076-1-IHC-anti-timp-2-antibody.jpgAnti-Metalloproteinase inhibitor 2 TIMP2 AntibodyAnti-TIMP2 antibody&#44; PA1076&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1076-ijn-12-263fig5.jpgAnti-Metalloproteinase inhibitor 2 TIMP2 AntibodyLong-term intravenous exposure to c-SWNTs promotes deposition of collagens and ECM remodeling by upregulating both MMP-2 and TIMP-2. Notes: Immunohistochemical examination of Col I and Col III, MMP-2, and TIMP-2 ( A ) in the rat lungs after intravenous injection at indicated periods (positive signal: brown; c-SWNT aggregations: red arrows; magnification: 100×). Mean optical density of Col I, Col III, MMP-2, and TIMP-2 ( B ) based on immunohistochemical assay. Data represent mean ± SEM. * P <0.05, ** P <0.01, and *** P <0.001 vs control. Abbreviations: c-SWNTs, carboxylated single-walled carbon nanotubes; ECM, extracellular matrix; MMP-2, matrix metalloproteinase-2; TIMP-2, tissue inhibitor of metalloproteinase-2; Col I, type-I collagen; Col III, type-III collagen; SEM, standard error of the mean.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5221802/&orig_host=www.ncbi.nlm.nih.gov'>28115845</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp3-antibody-pa1077-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1077-1-WB-anti-timp-3-antibody.jpgAnti-Metalloproteinase inhibitor 3 TIMP3 Antibody Picoband&reg;Anti-TIMP3 antibody&#44; PA1077&#44; Western blotting<br>Lane 1: Recombinant Human TIMP3 Protein 10ng<br>Lane 2: Recombinant Human TIMP3 Protein 5ng<br>Lane 3: Recombinant Human TIMP3 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp4-antibody-pa1078-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1078-1-WB-anti-timp-4-antibody.jpgAnti-Metalloproteinase inhibitor 4 TIMP4 Antibody Picoband&reg;Anti-TIMP4 antibody&#44; PA1078&#44; Western blotting<br>All lanes: Anti TIMP4 (PA1078) at 0.5ug/ml<br>Lane 1: Rat Kidney Tissue Lysate at 50ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 25KD<br>Observed bind size: 25KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1078-2-IHC-anti-timp-4-antibody.jpgAnti-Metalloproteinase inhibitor 4 TIMP4 Antibody Picoband&reg;Anti-TIMP4 antibody&#44; PA1078&#44; IHC(P)<br> IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1078-3-IHC-anti-timp-4-antibody.jpgAnti-Metalloproteinase inhibitor 4 TIMP4 Antibody Picoband&reg;Anti-TIMP4 antibody&#44; PA1078&#44; IHC(P)<br> IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp4-antibody-pa1078-1-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1078-1-1-WB-anti-timp-4-antibody.jpgAnti-Metalloproteinase inhibitor 4 TIMP4 Antibody Picoband&reg;Anti-TIMP4 antibody&#44; PA1078-1&#44; Western blotting<br>Lane 1: HT1080 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: SMMC Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegf-antibody-pa1080-boster.html2026-03-24T05:03:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-13048_2025_1679_fig5_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;YJD affected the VEGF/VEGFR-2/FAK pathway in vivo. ( A – B ) Germ cell markers MVH and Oct4 were detected by IF. ( C ) The expression of VEGF, VEGFR-2, and FAK was detected by qRT-PCR. ( D ) VEGF, VEGFR-2, FAK expression in the ovaries was examined by IHC; Black arrows point to areas of positive staining. ( E - F ) The expression of VEGF, VEGFR-2, FAK, p-VEGFR-2 and p-FAK were assessed by WB. ** P < 0.01, *** P < 0.001 vs. Control; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. TP; & P < 0.05, && P < 0.01 vs. TP + M-YJD <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-025-01679-2'>40287776</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-13048_2025_1679_fig6_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;YJD affected the VEGF/VEGFR-2/FAK pathway and KGN cell injury in vitro. ( A ) Screening of YJD for medicated serum concentrations utilized the CCK-8 assay. ( B ) The cell viability of each group was measured by CCK-8. ( C – G ) Serum levels of the hormones P, E2, FSH, LH, and AMH were examined by ELISA. ( H ) The expression of VEGF, VEGFR-2, and FAK was detected by qRT-PCR. ( I - J ) The expression of VEGF, VEGFR-2, FAK, p-VEGFR-2 and p-FAK were assessed by WB. ( K ) Apoptosis index was detected by Annexin V-FITC flow cytometry. ( L ) The expression of Bcl-2, pro-Caspase-3, Caspase-3 and AIF were assessed by WB. ** P < 0.01, *** P < 0.001 vs. Control; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. TP <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-025-01679-2'>40287776</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-13048_2025_1679_fig7_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;YJD affected POF by regulating the VEGF/VEGFR-2/FAK signaling pathway. ( A – E ) Serum levels of the hormones P, E2, FSH, LH, and AMH were examined by ELISA. Control and TP groups were set up, and the remaining groups were co-treated with TP and 15% medicated serum, along with VEGF inhibitor (Avastin), VEGFR-2 inhibitor (SU5408), or FAK inhibitor (Y15). ( F - G ) Serum levels of indicators of oxidative stress, MDA and SOD were assessed by ELISA. ( H - J ) The expression of VEGF, VEGFR-2, and FAK was detected by qRT-PCR. ( K - L ) The expression of VEGF, VEGFR-2, FAK, p-VEGFR-2 and p-FAK were assessed by WB. ( M ) Apoptosis rate was detected by Annexin V-FITC flow cytometry. ( N ) The expression of Bcl-2, pro-Caspase-3, Caspase-3 and AIF were assessed by western blotting. ** P < 0.01, *** P < 0.001 vs. Control; ## P < 0.01, ### P < 0.001 vs. TP; & P < 0.05, && P < 0.01, &&& P < 0.001 vs. TP + 15% medicated serum <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13048-025-01679-2'>40287776</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-fphar-15-1487183-g005.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;The promotion of NA for the expression of CD31 and VEGF in vivo . (A 1 –A 3 ) Immunofluorescence staining indicating the CD31 expression in the healed skins on 11 days post-treatment. (A 4 ) The semi-quantification of CD31 in vivo on day 11. (B 1 –B 3 ) Immunofluorescence staining indicating the VEGF expression in the healed skins on 11 days post-treatment. (B 4 ) The semi-quantification of VEGF in vivo on day 11. Statistical significance is indicated as ns p ˃ 0.05, **** p < 0.0001 versus the Control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1487183/full'>39502529</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-12951_2023_2204_fig1_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;Schematic representation of PDGF-BB-primed MSC transplantation by UTMD for infarcted myocardium repair. UTMD enhanced the delivery of MSCs into the infarcted myocardium by upregulating SDF-1 expression. Compared with UTMD alone, UTMD combined with PDGF-BB pretreatment increased the therapeutic effect of grafted cells by improving MSC migration and survival, reducing cardiomyocyte apoptosis, decreasing fibrosis, increasing microvessel density (via upregulation of VEGF, bFGF and IGF-1) and improving cardiac function. PDGF-BB promotes the survival/retention and cardioprotection of engrafted MSCs in rat models of MI via the PI3K/Akt pathway and CXCR4 activation <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-023-02204-7'>38102643</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-12951_2023_2204_fig6_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;Transplantation of PDGF-BB-primed MSCs via UTMD reduces cardiomyocyte apoptosis and improves angiogenesis in rat hearts post-MI. ( A ) Representative images of TUNEL-positive cardiomyocytes in the ischemic area 30 days after MI. Apoptotic nuclei were identified as TUNEL positive (green fluorescence), and total nuclei were identified by DAPI counterstaining (blue fluorescence). Myocardium was stained using a monoclonal antibody against cardiac troponin I (red fluorescent). Bar, 20 μm. ( B ). Quantification of TUNEL-positive cardiomyocytes. ( C - F ) Western blotting of activated caspase 3, Bax, and BCL-2 in the ischemic heart. GAPDH was used as a loading control. ( G ) Representative images of CD31 staining and α-SMA staining in the ischemic hearts of rats 30 days post-MI. Bar, 20 μm. ( H ) Quantitative analysis of the capillary density in the ischemic heart. (I) Quantitative analysis of the arteriole density in the ischemic heart. ( J - M ) Protein expression of VEGF, bFGF and IGF-1 determined by Western blotting in ischemic myocardium, with GAPDH as the internal control. Values are the mean ± SEM. Significant differences were determined by using one-way ANOVA. N = 5/group. *p < 0.01 vs. MI; # p < 0.01 vs. MI-MSC-vehicle <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-023-02204-7'>38102643</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2021_85295_fig5_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;Effect of miR-542-3p overexpression on angiogenetic capacity of HESCs. ( a ) q-PCR analysis of VEGF, MMP9.COX2 mRNA levels in control or miR-542-3p mimic or mimics NC groups. ( b ) Representative western blots showing VEGF, MMP9.COX2 protein levels in control or miR-542-3p mimic or mimics NC groups. ( c ) Capillary-like tube formation performed by HUVECs with /without the supernatant of miR-542-3p-overexpressing HESCs. Representative images (100X) are showed. Number of Tube area (tube meshes and total master segment length) were acquired automatically. *p < 0.05, **p < 0.01. Values are expressed as mean ± SD of three independent experiments. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-85295-2'>33785768</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-13287_2018_917_fig4_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;Effects of deer TMSB10 on cell proliferation, tube formation, and motility of HUVECs as well as on DRG outgrowth. a HUVECs overexpressing deer TMSB10 compared with vector alone. HUVECs overexpressing deer TMSB10 had significantly higher growth rates than that of the vector control group. b Determination of HUVEC proliferation using the MTT assay. HUVEC cells were exposed for 24, 48, and 72 h in the presence of deer exogenous TMSB10 (eTMSB10: 50, 100, or 150 ng/ml) and compared with control. The growth rates of the HUVECs treated with deer eTMSB10 (50, 100, and 150 ng/ml) were significantly higher than in controls. c Representative images (inverted phase contrast) of tube formation assays. Scale bars = 200 μm. d Quantification of tube formation by calculating the average number of branched vessels per field of view in response to exogenous TMSB10 (eTMSB10: 50, 100, or 150 ng/ml), overexpressed TMSB10 (TMSB10), and vascular endothelial growth factor (VEGF) as a positive control. Results showed that deer eTMSB10 and deer TMSB10 overexpressing HUVECs showed more tube formation in the HUVECs compared with controls or vector. e Transwell migration assays with HUVECs treated with deer eTMSB10 (50, 100, and 150 ng/ml) or overexpressing deer TMSB10 were compared with their controls. Results showed that deer eTMSB10 and deer TMSB10 overexpressing HUVECs showed more migration compared with the control or vector. f Lamellipodium emerging from a DRG neuron after treatment for 2 days or 5 days with 50, 100, and 150 ng/ml eTMSB10 or nerve growth factor (NGF; 50 ng/ml) as a positive control. Scale bar = 100 μm. g Quantification of average length of lamellipodium. Results showed that deer eTMSB10 significantly increased the growth of lamellipodium from DRG compared with the control. Data represent mean ± SD of three or four experiments. * P < 0.05, ** P < 0.01, compared with control or vector (control or vector control = 100%). OD optical density <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-018-0917-y'>29921287</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-13287_2018_917_fig5_html.pngAnti-VEGF/VEGFA Antibody Picoband&reg;Vascular endothelial growth factor (VEGF) ligands and receptors were found to be essential for the angiogenesis-promoting role of deer TMSB10 in HUVECs. a Representative images of VEGFA, VEGFB, VEGFC, VEGFD, FLT1, KDR, and FLT4 protein levels in HUVECs overexpressing deer TMSB10 after Western blot analysis. b Quantification of Western blotting for the VEGF family. The protein levels were normalized with GAPDH. The relative levels of proteins were calculated and plotted. The data are expressed as mean ± SD, n = 3 experiments. * P < 0.05, ** P < 0.01, compared with vector (control set at 100%). c VEGF family involved in the angiogenic process <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-018-0917-y'>29921287</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-medscimonit-23-3932-g006.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Regulation of HIF-1α expression by MTA1. ( A, B ) Real-time PCR and Western blot analysis results indicated that MTA1 correlates well with many lung development-related factors such as VEGF, AQP5, SPB, and β-catenin. ( C, D ) Western blot and real-time PCR showed significant upregulation of HIF-1α and VEGF in 293T and MLE-12 cells overexpressing MTA1 36 h after transfection. n=3, * P <0.05, ** P <0.01, *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5567764/&orig_host=www.ncbi.nlm.nih.gov'>28808223</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep36551_fig1_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Expression of CD31, IL-17, IL-6, IL-8, and VEGF protein in human lung adenocarcinoma tissues. Immunohistochemical determination of CD31, IL-17, IL-6, IL-8, VEGF protein in 30 patients with lung adenocarcinoma. ( A – F ) High CD31, IL-17, IL-6, IL-8, VEGF protein expression were presented in tumor tissue of case 58. ( G – L ) Low CD31, IL-17, IL-6, IL-8, VEGF protein expression were showed in tumor tissue of case 34. No staining was observed when an isotype-matched control mAb ( A , G ) was used (magnification, × 200). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep36551'>27819281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep36551_fig2_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Correlation between MVD and IL-17, IL-6, IL-8, VEGF in human lung adenocarcinoma tissues. IL-17, IL-6, IL-8, VEGF mRNA and protein and CD31 protein levels were determined in human lung adenocarcinoma tissues by qRT-PCR or IHC, respectively. (A) Spearman’s correlation analysis was performed to analyse the correlation between IL-17 (a), IL-6 (b), IL-8 (c), VEGF (d) protein expression and tumour microvessel density (MVD) by CD31 staining in tumor tissues with human lung adenocarcinoma. (B) Spearman’s correlation analysis was performed to analyse the correlation between IL-17 (a), IL-6 (b), IL-8 (c), VEGF (d) expression and tumour microvessel density by CD31 staining in 28 tissues with human lung adenocarcinoma. mRNA expression levels were calculated using the −ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep36551'>27819281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-73529-g001.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Increased expression of TSP-1, TGF-β1, CTGF and VEGF in supernatants of RA-FLS and HDMECs co-culture compared to NH-FLS and HDMECs co-culture. Normal human (NH) FLS and rheumatoid arthritis (RA) FLS were co-cultured with HDMECs for 48 h, respectively. A. ELISA analysis demonstrated significant increase in the concentrations of TSP-1, TGF-β1, CTGF and VEGF in supernatants of RA-FLS and HDMECs co-culture ( n = 3) compared with those from NH-FLS and HDMECs co-culture ( n = 3; p < 0.05). B. Real-time PCR analysis showed increased mRNA expression of TSP-1, TGF-β1, CTGF and VEGF in RA-FLS co-cultured ( n = 3) compared to NH-FLS co-cultured ( n = 3; * p < 0.05, ** p < 0.01, *** p < 0.001). C. and D. Transwell assay (C; n = 3) and tube formation test (D; n = 3) for 6 h demonstrated significant up-regulation in migration and capillary-like structure formation of HDMECs respectively under treatment of supernatants from RA-FLS and HDMECs co-culture ( n = 3) compared to those from NH-FLS and HDMECs co-culture ( n = 3; * p < 0.05). E. aortic rings were placed on GFR-Matrigel-coated plates and incubated in 1% FBS EGM-2. On 3 rd day, the EGM-2 were exchanged with supernatants from FLS and HDMECs co-cuture and further incubated for 3 days. Ex vivo aortic ring angiogenesis assay showed significant up-regulation in microvessel sprouting under treatment of supernatants from RA-FLS and HDMECs co-culture ( n = 3) compared to those from NH-FLS and HDMECs co-culture ( n = 3; * p < 0.05). Bars = 300 μm. Original magnification = ×5. Results are expressed as the mean ± S.E.M.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>29088724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep36551_fig3_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Correlation between IL-17 and IL-6, IL-8, VEGF in human lung adenocarcinoma tissues. IL-17, IL-6, IL-8, VEGF mRNA and protein protein levels were determined in human lung adenocarcinoma tissues by qRT-PCR or IHC, respectively. (A) Spearman’s correlation analysis was performed to analyse the correlation between IL-17 protein expression and IL-6 (a), IL-8 (b), VEGF (c) protein in tumour tissues of patients with lung adenocarcinoma. (B) Pearson’s correlation analysis was used to analyse the relationship between IL-17 mRNA expression and IL-6 (a), IL-8 (b), VEGF (c) mRNA expression in tumour tissues of patients with lung adenocarcinoma.. mRNA expression levels were calculated using the −ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep36551'>27819281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-73529-g002.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;The modulation of TSP-1, TGF-β1, CTGF and VEGF expression alongside interventions of TSP-1, TGF-β1, CTGF and VEGF stimulation or knockdown. A. The effects of TSP-1, TGF-β1, CTGF and VEGF stimulation/silencing in RA-FLS on TSP-1, TGF-β1, CTGF, and VEGF proteins expression in the supernatants were determined by ELISA. RA-FLS were stimulated with CTGF (500 ng/ml), TGF-β1 (5ng/ml), TSP-1 (1,000 ng/ml) and VEGF (50 ng/ml) respectively for 48 h. Stimulation ( n = 3)/silencing ( n = 3) of TGF-β1 in RA-FLS resulted in significant increase/decrease in the supernatants protein expression of TSP-1, CTGF, and VEGF ( p < 0.05). Stimulation ( n = 3)/silencing ( n = 3) of VEGF in RA-FLS leaded to similar changes of the other three protein expression in supernatants ( p < 0.05). Addition(n = 3)/silencing( n = 3) of CTGF in RA-FLS caused significant up-/down-regulation in the supernatants protein expression of TSP-1 and VEGF ( p < 0.05). And stimulation ( n = 3)/knockdown ( n = 3) of TSP-1 in RA-FLS didn’t lead to significant increase/decrease in the expression of supernatants protein TGF-β1, CTGF and VEGF. B. TSP-1, TGF-β1, CTGF and VEGF mRNA expression levels were analyzed by real-time PCR after TSP-1, TGF-β1, CTGF and VEGF stimulation/silencing. Results showed similar alterations of TSP-1, TGF-β1, CTGF and VEGF mRNA expression as demonstrated in supernatants protein regulation after TSP-1, TGF-β1, CTGF and VEGF stimulation/silencing ( n = 3 respectively; p < 0.05). Results are expressed as the mean ± S.E.M. * p < 0.05, ** p < 0.01, *** p < 0.001 versus Veh, # p < 0.05, ## p < 0.01, ### p < 0.001 versus Scr siRNA. Veh = vehicle control. Scr siRNA = scramble siRNA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>29088724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep36551_fig4_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;The effect of IL-17 on IL-6, IL-8, VEGF expression in human lung adenocarcinoma cells in vitro . A549/H292 cells were incubated with IL-17 or IL-17 (100 ng/ml) for 6 or 48 h. The IL-6, IL-8, and VEGF mRNA and protein levels were determined by qRT-PCR or ELISA, respectively. (A) IL-6, IL-8, and VEGF mRNA levels in A549/H292 cells mRNA expression levels were calculated using the 2 −ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene. The data are presented as the mean ± SEM of three independent experiments. (B) IL-6, IL-8, and VEGF protein levels in A549/H292 cells by ELISA. The results shown are representative of four independent experiments and are presented the mean ± SEM. Comparisons were performed using the t-test. *p < 0.05; **p < 0.01; and ***p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep36551'>27819281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-73529-g003a.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;As 2 O 3 inhibited angiogenesis by modulating TSP-1, TGF-β1, CTGF and VEGF expression in RA-FLS. RA-FLS and HDMECs co-cultures were respectively treated with As 2 O 3 alone or together with TNF-α (100ng/ml) for 48 h. A. TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures were analyzed by ELISA. Results showed that concentrations of TSP-1, TGF-β1, CTGF and VEGF increased significantly after treatment with TNF-α (100 ng/ml) ( n = 3) compared to vehicle control group ( n = 3; & p < 0.05), and then significantly decreased concentrations of TSP-1, TGF-β1, CTGF and VEGF were observed after the treatment of As 2 O 3 at doses of 1.0 μM and 2.0 μM ( n = 3, respectively; # p < 0.05, ## p < 0.01). TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures without TNF-α addition also decreased significantly after treatment of As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) compared to vehicle control ( n = 3; * p < 0.05). B. mRNA levels of TSP1, TGF-β1, CTGF and VEGF expression in RA-FLS co-cultured were performed by real-time PCR. Results showed similar changes of TSP-1, TGF-β1, CTGF and VEGF mRNA expression as demonstrated in protein regulation after treatment of As 2 O 3 alone ( n = 3) or together with TNF-α (100ng/ml) ( n = 3; * p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01). C. and D. Transwell assay and tube formation test were performed by applying supernatants from RA-FLS and HDMECs co-culture to HDMECs for 6 h respectively. Results showed that migration and capillary-like structure formation of HDMECs significantly increased after TNF-α (100ng/ml) stimulation ( n = 3, respectively; * p < 0.05), while As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) played a significantly opposite role in migration and tube formation with or without TNF-α (* p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01), which were also in a dose dependent manner. E. Ex vivo aortic ring angiogenesis assay showed similar changes of microvessel sprouting as demonstrated in migration and tube formation of HDMECs in the transwell assay and tube formation test above ( n = 3, respectively; * p < 0.05, ** p < 0.01, # p < 0.05). Bars = 300μm. Original magnification = ×5. Results are expressed as the mean ± S.E.M. & p < 0.05 versus Veh. * p < 0.05, ** p < 0.01 versus Veh, # p < 0.05, ## p < 0.01 versus TNF-α. Veh = vehicle control under treatment of 1% FBS DMEM alone. TNF-α = control group under treatment of TNF-α (100ng/ml).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>29088724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-73529-g003b.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;As 2 O 3 inhibited angiogenesis by modulating TSP-1, TGF-β1, CTGF and VEGF expression in RA-FLS. RA-FLS and HDMECs co-cultures were respectively treated with As 2 O 3 alone or together with TNF-α (100ng/ml) for 48 h. A. TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures were analyzed by ELISA. Results showed that concentrations of TSP-1, TGF-β1, CTGF and VEGF increased significantly after treatment with TNF-α (100 ng/ml) ( n = 3) compared to vehicle control group ( n = 3; & p < 0.05), and then significantly decreased concentrations of TSP-1, TGF-β1, CTGF and VEGF were observed after the treatment of As 2 O 3 at doses of 1.0 μM and 2.0 μM ( n = 3, respectively; # p < 0.05, ## p < 0.01). TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures without TNF-α addition also decreased significantly after treatment of As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) compared to vehicle control ( n = 3; * p < 0.05). B. mRNA levels of TSP1, TGF-β1, CTGF and VEGF expression in RA-FLS co-cultured were performed by real-time PCR. Results showed similar changes of TSP-1, TGF-β1, CTGF and VEGF mRNA expression as demonstrated in protein regulation after treatment of As 2 O 3 alone ( n = 3) or together with TNF-α (100ng/ml) ( n = 3; * p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01). C. and D. Transwell assay and tube formation test were performed by applying supernatants from RA-FLS and HDMECs co-culture to HDMECs for 6 h respectively. Results showed that migration and capillary-like structure formation of HDMECs significantly increased after TNF-α (100ng/ml) stimulation ( n = 3, respectively; * p < 0.05), while As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) played a significantly opposite role in migration and tube formation with or without TNF-α (* p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01), which were also in a dose dependent manner. E. Ex vivo aortic ring angiogenesis assay showed similar changes of microvessel sprouting as demonstrated in migration and tube formation of HDMECs in the transwell assay and tube formation test above ( n = 3, respectively; * p < 0.05, ** p < 0.01, # p < 0.05). Bars = 300μm. Original magnification = ×5. Results are expressed as the mean ± S.E.M. & p < 0.05 versus Veh. * p < 0.05, ** p < 0.01 versus Veh, # p < 0.05, ## p < 0.01 versus TNF-α. Veh = vehicle control under treatment of 1% FBS DMEM alone. TNF-α = control group under treatment of TNF-α (100ng/ml).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>29088724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-73529-g003c.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;As 2 O 3 inhibited angiogenesis by modulating TSP-1, TGF-β1, CTGF and VEGF expression in RA-FLS. RA-FLS and HDMECs co-cultures were respectively treated with As 2 O 3 alone or together with TNF-α (100ng/ml) for 48 h. A. TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures were analyzed by ELISA. Results showed that concentrations of TSP-1, TGF-β1, CTGF and VEGF increased significantly after treatment with TNF-α (100 ng/ml) ( n = 3) compared to vehicle control group ( n = 3; & p < 0.05), and then significantly decreased concentrations of TSP-1, TGF-β1, CTGF and VEGF were observed after the treatment of As 2 O 3 at doses of 1.0 μM and 2.0 μM ( n = 3, respectively; # p < 0.05, ## p < 0.01). TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures without TNF-α addition also decreased significantly after treatment of As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) compared to vehicle control ( n = 3; * p < 0.05). B. mRNA levels of TSP1, TGF-β1, CTGF and VEGF expression in RA-FLS co-cultured were performed by real-time PCR. Results showed similar changes of TSP-1, TGF-β1, CTGF and VEGF mRNA expression as demonstrated in protein regulation after treatment of As 2 O 3 alone ( n = 3) or together with TNF-α (100ng/ml) ( n = 3; * p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01). C. and D. Transwell assay and tube formation test were performed by applying supernatants from RA-FLS and HDMECs co-culture to HDMECs for 6 h respectively. Results showed that migration and capillary-like structure formation of HDMECs significantly increased after TNF-α (100ng/ml) stimulation ( n = 3, respectively; * p < 0.05), while As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) played a significantly opposite role in migration and tube formation with or without TNF-α (* p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01), which were also in a dose dependent manner. E. Ex vivo aortic ring angiogenesis assay showed similar changes of microvessel sprouting as demonstrated in migration and tube formation of HDMECs in the transwell assay and tube formation test above ( n = 3, respectively; * p < 0.05, ** p < 0.01, # p < 0.05). Bars = 300μm. Original magnification = ×5. Results are expressed as the mean ± S.E.M. & p < 0.05 versus Veh. * p < 0.05, ** p < 0.01 versus Veh, # p < 0.05, ## p < 0.01 versus TNF-α. Veh = vehicle control under treatment of 1% FBS DMEM alone. TNF-α = control group under treatment of TNF-α (100ng/ml).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>29088724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-73529-g003d.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;As 2 O 3 inhibited angiogenesis by modulating TSP-1, TGF-β1, CTGF and VEGF expression in RA-FLS. RA-FLS and HDMECs co-cultures were respectively treated with As 2 O 3 alone or together with TNF-α (100ng/ml) for 48 h. A. TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures were analyzed by ELISA. Results showed that concentrations of TSP-1, TGF-β1, CTGF and VEGF increased significantly after treatment with TNF-α (100 ng/ml) ( n = 3) compared to vehicle control group ( n = 3; & p < 0.05), and then significantly decreased concentrations of TSP-1, TGF-β1, CTGF and VEGF were observed after the treatment of As 2 O 3 at doses of 1.0 μM and 2.0 μM ( n = 3, respectively; # p < 0.05, ## p < 0.01). TSP-1, TGF-β1, CTGF and VEGF protein expression in the supernatants from RA-FLS and HDMECs co-cultures without TNF-α addition also decreased significantly after treatment of As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) compared to vehicle control ( n = 3; * p < 0.05). B. mRNA levels of TSP1, TGF-β1, CTGF and VEGF expression in RA-FLS co-cultured were performed by real-time PCR. Results showed similar changes of TSP-1, TGF-β1, CTGF and VEGF mRNA expression as demonstrated in protein regulation after treatment of As 2 O 3 alone ( n = 3) or together with TNF-α (100ng/ml) ( n = 3; * p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01). C. and D. Transwell assay and tube formation test were performed by applying supernatants from RA-FLS and HDMECs co-culture to HDMECs for 6 h respectively. Results showed that migration and capillary-like structure formation of HDMECs significantly increased after TNF-α (100ng/ml) stimulation ( n = 3, respectively; * p < 0.05), while As 2 O 3 at doses of 1.0 μM ( n = 3) and 2.0 μM ( n = 3) played a significantly opposite role in migration and tube formation with or without TNF-α (* p < 0.05, ** p < 0.01, # p < 0.05, ## p < 0.01), which were also in a dose dependent manner. E. Ex vivo aortic ring angiogenesis assay showed similar changes of microvessel sprouting as demonstrated in migration and tube formation of HDMECs in the transwell assay and tube formation test above ( n = 3, respectively; * p < 0.05, ** p < 0.01, # p < 0.05). Bars = 300μm. Original magnification = ×5. Results are expressed as the mean ± S.E.M. & p < 0.05 versus Veh. * p < 0.05, ** p < 0.01 versus Veh, # p < 0.05, ## p < 0.01 versus TNF-α. Veh = vehicle control under treatment of 1% FBS DMEM alone. TNF-α = control group under treatment of TNF-α (100ng/ml).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>29088724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-73529-g006.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;As 2 O 3 suppressed TSP-1, TGF-β1, CTGF and VEGF expression and microvessel density in synovial tissue of CIA mice. Positive staining appears as brown color. A. - D. , F. Immunohistochemical analysis demonstrated increased percentage (%) of positive cells for CTGF, TGF-β1, TSP-1 and VEGF in synovial tissue of CIA mice ( n = 6; A-D, II, CIA control mice) compared to synovium of normal mice ( n = 6; A-D, I, normal mice; F, *** p < 0.001), while CIA mice treated with As 2 O 3 at a dose of 5.0 mg/kg/day ( n = 6; A-D, III) and MTX 1.5mg/kg/week ( n = 6; A-D, IV) showed decreased % of positive cells for CTGF, TGF-β1, TSP-1 and VEGF in synovial tissue compared to CIA control mice ( n = 6; A-D, II; F, ### p < 0.001). Original magnification = ×40. Bars = 25μm. E. , G. Immunohistochemical analysis for vWF showed a significant increase in number of microvessels in synovial tissue of CIA control mice ( n = 6; E, II) compared to the synovium of normal mice ( n = 6; E, I; G, *** p < 0.001), while CIA mice treated with As 2 O 3 at a dose of 5.0 mg/kg/day ( n = 6; E, III) and MTX 1.5 mg/kg/week ( n = 6; E, IV) demonstrated decreased number of microvessels in synovial tissue compared to CIA control mice ( n = 6; E, II; G, ## p < 0.01). Original magnification = ×40. Bars = 25μm. Data are expressed as the mean ± S.E.M. *** p < 0.001 versus NC, # p < 0.05, ## p < 0.01, ### p < 0.001 versus Con. NC = normal control mice. Con = CIA control mice. MTX = methotrexate.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>29088724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep36551_fig6_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;STAT1 inhibitor attenuated IL-17-induced IL-6, IL-8, and VEGF production in human lung adenocarcinoma. A549/H292 cells were incubated with IL-17 or IL-17 plus a STAT1 inhibitor for 6 or 48 h (100 ng/ml IL-17; and 30 μM inhibitor). The IL-6, IL-8, and VEGF mRNA and protein levels were determined by qRT-PCR and ELISA, respectively. (A) IL-6, IL-8, and VEGF mRNA levels in A549/H292 cells, mRNA expression levels were calculated using the 2 −ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene. The data are presented as the mean ± SEM of three independent experiments. (B) IL-6, IL-8, and VEGF protein levels in A549/H292 cells. The data are presented as the mean ± SEM of three independent experiments. Comparisons were performed using the t-test. *p < 0.05; **p < 0.01; and ***p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5650279/&orig_host=www.ncbi.nlm.nih.gov'>27819281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep36551_fig7_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;STAT1 siRNA reversed IL-17-induced IL-6, IL-8, and VEGF expression in human lung adenocarcinoma cells in vitro . A549/H292 cells (2 × 10 5 cells/well) were transfected with STAT1 siRNA for 48 h. (A) STAT1 protein expression was determined by WB. Then, A549/H292 cells (5 × 10 5 cells/well) were transfected with siRNA for 48 h and subsequently treated with human IL-17 (100 ng/ml) for an additional 6 or 48 h. The IL-6, IL-8, and VEGF mRNA and protein expression levels were determined by qRT-PCR and WB, respectively. (B) IL-6, IL-8, VEGF mRNA levels in A549/H292 cells. mRNA expression levels were calculated using the 2 −ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene. The data are presented as the mean ± SEM of three independent experiments. (C) IL-6, IL-8, and VEGF protein levels in A549/H292 cells and the results are presented as the mean ± SEM of three independent experiments. Comparisons were performed using the t-test. *p < 0.05; **p < 0.01; and ***p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep36551'>27819281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep36551_fig9_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;IL-6, IL-8, VEGF and STAT1 expression were augmented in A549-IL-17 cell-bearing nude tissues. (A , B) The relative protein expression of IL-6, IL-8, VEGF and STAT1 in tumour tissues of A549-IL-17 vs. A549-Neo cell-bearing nude mice was determined by WB. (C) The relative mRNA expression of IL-6, IL-8, VEGF and STAT1 in tumour tissues of A549-IL-17 vs. A549-Neo cell-bearing nude mice was determined by qRT-PCR (n = 5). mRNA expression levels were calculated using the 2 −ΔΔCt method, and target gene expression was normalized to the GAPDH housekeeping gene. The data are presented as the mean ± SEM for five mice per group, and the results are representative of two independent experiments. *p < 0.05; **p < 0.01; and ***p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep36551'>27819281</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-oncotarget-08-22406-g003.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Increased VEGF and VEGFR2 expression in tumors from CD47-deficient mice. ( A ) Representative images of tumor sections stained for VEGF-A (brown). Scale bar, 20 μm. ( B ) Percentages of VEGFR2 + area by counting positive cells in six randomly selected fields (400×) using Image Pro Plus 6.0 software. ( C ) mRNA levels of VEGF-A in tumor samples from WT and CD47 −/− mice ( n = 4 per group). ( D ) Representative images of tumor sections stained for VEGFR2 + (brown). Scale bar, 20 μm. ( E ) Percentages of VEGFR2 + area by counting positive cells in six randomly selected fields (400×) using Image Pro Plus 6.0 software. ( F ) mRNA levels of VEGFR2 in tumor samples from WT and CD47 −/− mice ( n = 4 per group). Data are mean ± SDs. * P < 0.05, ** P < 0.01; *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5410232/&orig_host=www.ncbi.nlm.nih.gov'>27283989</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-41598_2016_article_bfsrep26722_fig7_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;The anti-angiogenic activity of SPS on HUVECs in vitro . ( a ) Dose- and time-dependent growth-inhibiting effects of SPS on HUVECs. Cells were cultured in 96-well plate and treated with different concentrations of SPS (0–1.5 mg/ml) for 12 and 24 h. The cell viability was analyzed by MTT assay. Each data indicated the mean ± SD of three independent experiments (n = 3). * P < 0.05, ** P < 0.01 versus medium control. After A549 cells were treated without (I) or with 0.2 (II), 0.3 (III) and 0.4 (IV) mg/ml of SPS for 8–10 h, cell migration was analyzed using scratch-wound assay ( b ) as well as Transwell assay ( c ). Quantitative evaluations of HUVECs migration induced by SPS in the scratch-wound assay ( d ) and Transwell assay ( e ). ( f ) SPS inhibited the formation of new capillary tube of HUVECs. HUVECs were plated on the surface of the Matrigel in a 96-well plate, where endothelial cells could align and form cords and treated without (I) or with 0.2 (II), 0.3 (III) and 0.4 (IV) mg/ml of SPS for 6 h. ( g ) SPS did not disrupt the preformed vascular network of HUVECs. After the new tube-like structures established, various concentrations of SPS, 0 (I), 0.2 (II), 0.4 (III) and 0.6 (IV) mg/ml were added and incubated for another 12 h. The disruption on the preformed tubes was observed and recorded using an inverted microscope. ( h ) After treated with SPS for 24 h, HUVECs were lysed and western blotting analysis was performed to detect the expressions of VEGF and VEGFR. ( i ) Histograms showed the quantitative evaluation of VEGF and VEGFR protein levels, which were measured by Image J and results were normalized to untreated cells. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep26722'>27216943</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-13046_2011_article_497_fig5_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;RT-PCR analysis of human and chicken angiogenic factors mRNA . Microarray analysis was performed to screen out the angiogenic factors affected by HIF-1α in SCLC cells (table 2). Afterwards, RT-PCR analysis was used to detect the expression of angiogenic factors affected by HIF-1a in the transplantation tumors of CAM in vivo . (A), Human and chicken VEGF-A, TNFAIP6, PDGFC, FN1, MMP28, MMP14, SOCS2 and IGFBP3 mRNA expression: Representative images of three independent experiments (Lane 1: control group-no human mRNA expression, Lane 2: transplantation tumor of NCI-H446 cells transduction by empty vector Ad5-NCI-H446 cells group, Lane 3: ransplantation tumor of NCI-H446 cells with transduction by HIF-1α-NCI-H446/HIF-1α group, Lane 4: transplantation tumor of NCI-H446 cells with transduction by siHIF-1α-NCI-H446/siHIF-1α group). (B and C), Relative expression levels of mRNA in NCI-H446/HIF-1α group and NCI-H446/siHIF-1α group compared with that in control group and NCI-H446 cells group (p < 0.05). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-30-77'>21843314</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-13046_2011_article_497_fig7_html.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Western blot analysis of the human and chicken VEGF-A protein in the CAM . In the NCI-H446/HIF-1α and NCI-H446/siHIF-1α groups, the SCLC cells were transduced with Ad-HIF-1α or Ad-siHIF-1α (MOI = 50) for 60 h before implanting onto the CAM to form transplantation tumors. Western blots were performed to detect the VEGF-A protein level in the tumors and peripheral tissues on day 17 of incubation. Data are presented as means ± SD. (A) Representative images of three independent experiments (Lane A - human VEGF-A protein expression in the tumors from the NCI-H446 group; Lane B - human VEGF-A protein expression in the tumors from the NCI-H446/HIF-1α group; and Lane C - human VEGF-A protein expression in the tumors from the NCI-H446/siHIF-1α group) (human - * p < 0.05 group C vs. group B; ** p < 0.05 group C vs. group D) (chicken - * p < 0.05 group C vs. group B; ** p < 0.05 group C vs. group D). (B) Representative images of three independent experiments (Lane A - chicken VEGF-A protein expression of control group; Lane B - chicken VEGF-A protein expression in the tumors from the NCI-H446 group; Lane C - chicken VEGF-A protein expression in the tumors from the NCI-H446/HIF-1α group; and Lane D - Chicken VEGF-A protein expression in tumors from the NCI-H446/siHIF-1α group). (C) Densitometry analysis of the relative expression of VEGF-A protein compared to the corresponding β-actin in each group (p < 0.05). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-30-77'>21843314</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-wjg-14-2308-g004.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;A: Effect of AMD3100 on expression of MMP-2, MMP-9 and VEGF in SW480 cells. Protein samples extracted from SW480 cells treated for 26 h with AMD3100 were subjected to Western blotting for MMP-2, MMP-9, VEGF and GAPDH proteins. AMD3100 significantly decreased MMP-9 and VEGF protein expression in SW480 cells in a dose-dependent manner. The level of GAPDH expression was determined as a control for equivalent protein loading; B: RNA samples extracted from SW480 cells treated for 26 h with AMD3100 were subjected to RT-PCR for MMP-2 , MMP-9 , VEGF and β -actin . AMD3100 also significantly decreased expression of MMP-9 and VEGF mRNAs in SW480 cells, and the inhibitory effect was dose-dependent. RT-PCR for β -actin was performed in parallel to show an equal amount of total RNA in the sample.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC2705083/'>18416455</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-gr4.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;ZGF Promotes VEGF/Notch1/Noggin Pathway Activation. (A) RT-PCR was used to detect NOG, Notch1, and VEGF mRNA expression levels in the tibial metaphysis of Wistar and GK rats. (B–C) Target proteins NOG, Notch1, and VEGF expression levels were measured by Western blot in the tibial metaphysis of Wistar and GK rats. (Uncropped Western blot images are included in Additional file 1.) Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 VS. MC group. #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 VS. PC group. ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, ^^^^P < 0.0001 VS. BC group, (n = 3). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)04045-3'>38524608</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-1-s2.0-s2590006425008841-gr2.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;CircPUM1 promotes angiogenesis in ovarian cancer as the form of exosomes. (A) The expression of circPUM1 was positively related with MVD in ovarian cancer tissue. (B) Experimental design schematic (Created in BioRender. Guan, X. (2025) https://BioRender.com/f5br0q0): HUVEC were co-cultured with exosomes derived from circPUM1-overexpressing and control OVCAR3 cells. (C) RT-qPCR showed that circPUM1 was highly expressed in HUVEC after co-cultured with exosomal circPUM1. Exosomal circPUM1 enhanced migration ability (D, E), viability (F), invasiveness (G), and tube formation ability (H) of HUVECs. (I) Circular RNA pull-down assays using biotinylated circPUM1 probes confirmed co-enrichment of circPUM1 and miR-607. (J) Dual-luciferase reporter assays showed miR-607 targeting 3′-UTR in both VEGFA and RAB27B. (K) Western blot revealed that overexpression of circPUM1 upregulated VEGFA and RAB27B, whereas miR-607 downregulated these targets in OVCAR3 cells. Exosomal circPUM1 elevated intracellular VEGFA expression in HUVECs. (L) Molecular schematic (Created in BioRender. Guan, X. (2025) https://BioRender.com/5eldsz5): In ovarian cancer, the highly expressed circPUM1 sponges miR-607, upregulating expression of VEGFA and RAB27B. This dual regulation establishes a pro-tumorigenic cascade through two distinct pathways: (1) RAB27B-mediated secretion of exosomal circPUM1, and (2) VEGFA-induced activation of angiogenic signaling.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2590006425008841'>41050095</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-1-s2.0-s2590006425008841-gr4.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;In vitro antitumor performance of P-Nb2C@si-circPUM1 in ovarian cancer cells. (A) Confocal microscopy images showed intracellular uptake of Cy3-labeled circPUM1 siRNA. Cell apoptosis assays (B), Transwell assays (C), wound healing assays (E) and EdU proliferation assays (F) showed that P-Nb2C-loaded circPUM1 siRNA effectively suppressed ovarian cancer cell phenotypes. (G) RT-qPCR demonstrated a significant downregulation of circPUM1 expression in lipo2000-transfected and P-Nb2C-loaded siRNA groups. Immunofluorescence (D) and Western blot (H) validated the downregulation of VEGFA and RAB27B expression in cells treated with P-Nb2C@si-circPUM1. (I) CCK8 assays showed inhibition of ovarian cancer cell viability in lipo2000-transfected and P-Nb2C-loaded siRNA groups.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2590006425008841'>41050095</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-1-s2.0-s2590006425008841-gr5.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;In vitro anti-angiogenic performance of P-Nb2C@si-circPUM1 in HUVECs. (A) QPCR showed a reduction of circPUM1 levels in exosomes derived from P-Nb2C@si-circPUM1-treated ovarian cancer cells. (B) Intracellular circPUM1 level in HUVEC treated with Exo-P-Nb2C@si-circPUM1 were markedly lower than those in Exo-control and Exo-P-Nb2C@si-scramble groups. CCK-8 and EdU assays demonstrated that Exo-P-Nb2C@si-circPUM1 suppressed cell viability (C) and DNA replication capacity (D, E) in HUVECs. Wound healing, Transwell assay and tube formation assay indicated that Exo-P-Nb2C@si-circPUM1 treatment significantly attenuated HUVEC migration (F), invasion (G), and angiogenic potential (I) compared to control groups. Immunofluorescence (H, J) and Western blot (K) confirmed downregulation of VEGFA in Exo-P-Nb2C@si-circPUM1 treated HUVECs.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2590006425008841'>41050095</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-1-s2.0-s2590006425008841-gr7.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Molecular mechanism validation of P-Nb2C-PEG@si-circPUM1 anti-angiogenic performance in vivo. (A) H&E staining showed increased necrotic loci within tumors of P-Nb2C-PEG@si-circPUM1 group. (B, D) IHC staining of Ki-67 demonstrated a clear reduction in Ki-67-positive cells in P-Nb2C-PEG@si-circPUM1 group. (C, F) IHC staining of CD31 indicated a marked decrease in MVD within tumors of P-Nb2C-PEG@si-circPUM1 group. (H) QPCR showed a significant downregulation of circPUM1 expression in the P-Nb2C-PEG@si-circPUM1 treated group. IHC (E, G) and Western blot (I, J) demonstrated significant down-regulation of RAB27B and VEGFA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2590006425008841'>41050095</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-vegfa-primary-antibodies-wb-testing-1.jpgAnti-VEGF/VEGFA Antibody Picoband&reg; Western blot analysis of VEGFA using anti-VEGFA antibody (PA1080). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFA antigen affinity purified polyclonal antibody (Catalog # PA1080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFA at approximately 27 kDa. The expected band size for VEGFA is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-vegfa-primary-antibodies-ihc-testing-2.jpgAnti-VEGF/VEGFA Antibody Picoband&reg; IHC analysis of VEGFA using anti-VEGFA antibody (PA1080). <br> VEGFA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGFA Antibody (PA1080) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1080-vegfa-primary-antibodies-ihc-testing-3.jpgAnti-VEGF/VEGFA Antibody Picoband&reg; IHC analysis of VEGFA using anti-VEGFA antibody (PA1080). <br> VEGFA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGFA Antibody (PA1080) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-skp2-antibody-pa1102-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1102-skp2-primary-antibodies-wb-testing-1.jpgAnti-SKP2 Antibody Picoband&reg;Western blot analysis of SKP2 using anti-SKP2 antibody (PA1102). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP2 antigen affinity purified polyclonal antibody (PA1102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SKP2 at approximately 48 kDa. The expected band size for SKP2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1102-skp2-primary-antibodies-if-testing-1.jpgAnti-SKP2 Antibody Picoband&reg;IF analysis of SKP2 using anti-SKP2 antibody (PA1102) and anti-Tubulin Alpha antibody (M03989-3).<br> SKP2 was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SKP2 Antibody (PA1102) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1102-skp2-primary-antibodies-fcm-testing-1.jpgAnti-SKP2 Antibody Picoband&reg;Flow Cytometry analysis of HepG2 cells using anti-SKP2 antibody (PA1102). <br> Overlay histogram showing HepG2 cells stained with PA1102 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SKP2 Antibody (PA1102, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lamin-a-c-antibody-pa1103-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1103-4-IHC-anti-lamin-a-c-antibody.jpgAnti-Lamin A + C/LMNA Antibody Picoband&reg;Anti-Lamin A+C antibody&#44; PA1103&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1103-lamin_ac-primary-antibodies-wb-testing-1.jpgAnti-Lamin A + C/LMNA Antibody Picoband&reg; Western blot analysis of Lamin A/C using anti-Lamin A/C antibody (PA1103). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: human CACO-2 whole cell lysates, <br> Lane 6: rat spleen tissue lysates, <br> Lane 7: mouse liver tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin A/C antigen affinity purified polyclonal antibody (Catalog # PA1103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin A/C at approximately 70-74KD. The expected band size for Lamin A/C is at 74KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1103-2-IHC-anti-lamin-a-c-antibody.jpgAnti-Lamin A + C/LMNA Antibody Picoband&reg;Anti-Lamin A+C antibody&#44; PA1103&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1103-3-IHC-anti-lamin-a-c-antibody.jpgAnti-Lamin A + C/LMNA Antibody Picoband&reg;Anti-Lamin A+C antibody&#44; PA1103&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp60-antibody-pa1106-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1106-hsp60-primary-antibodies-wb-testing-1.jpgAnti-HSP60/HSPD1 Antibody Picoband&reg; Western blot analysis of HSP60 using anti-HSP60 antibody (PA1106). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates.<br> Lane 5: rat lung tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse lung tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP60 antigen affinity purified polyclonal antibody (Catalog # PA1106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP60 at approximately 61 kDa. The expected band size for HSP60 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1106-2-IHC-anti-hsp60-antibody.jpgAnti-HSP60/HSPD1 Antibody Picoband&reg;Anti-HSP60 antibody&#44; PA1106&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1106-3-IF-anti-hsp60-antibody.jpgAnti-HSP60/HSPD1 Antibody Picoband&reg;Anti-HSP60 antibody&#44; PA1106&#44; ICC<br>ICC: SMMC Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1106-hspd1-primary-antibodies-ihc-testing-5.jpgAnti-HSP60/HSPD1 Antibody Picoband&reg;IHC analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (PA1106). <br>HSP60/HSPD1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP60/HSPD1 Antibody (PA1106) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1106-hspd1-primary-antibodies-ihc-testing-6.jpgAnti-HSP60/HSPD1 Antibody Picoband&reg;IHC analysis of HSP60/HSPD1 using anti-HSP60/HSPD1 antibody (PA1106). <br>HSP60/HSPD1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP60/HSPD1 Antibody (PA1106) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1106-hspd1-primary-antibodies-if-testing-4_1.jpgAnti-HSP60/HSPD1 Antibody Picoband&reg; IF analysis of HSPD1 using anti-HSPD1 antibody (PA1106). <br> HSPD1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- HSPD1 Antibody (PA1106) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1106-hspd1-primary-antibodies-fcm-testing-5_1.pngAnti-HSP60/HSPD1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-HSPD1 antibody (PA1106). <br>Overlay histogram showing A549 cells stained with PA1106 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPD1 Antibody (PA1106, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ifitm1-antibody-pa1112-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1112-1-WB-anti-ifitm1-antibody.jpgAnti-IFITM1 Antibody Picoband&reg;Anti-IFITM1 antibody&#44; PA1112&#44; Western blotting<br>Lane 1: SW620 Cell Lysate<br>Lane 2: CEM Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1112-2-IHC-anti-ifitm1-antibody.jpgAnti-IFITM1 Antibody Picoband&reg;Anti-IFITM1 antibody&#44; PA1112&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-2-antibody-pa1113-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1113-1-WB-anti-caspase-2-antibody.jpgAnti-Caspase-2/CASP2 Antibody Picoband&reg;Anti-Caspase-2 antibody&#44; PA1113&#44; Western blotting<br>Lane 1: CEM Cell Lysate<br>Lane 2: SMMC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-shp2-antibody-pa1114-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1114-1-WB-anti-shp2-antibody.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg;Anti-SHP2 antibody&#44; PA1114&#44; Western blotting<br>WB: JURKAT Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1114-2-IHC-anti-shp2-antibody.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg;Anti-SHP2 antibody&#44; PA1114&#44; IHC(P)<br>IHC(P): Human Thyroid Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gamma-catenin-antibody-pa1117-1-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1117-1-jup-primary-antibodies-ihc-testing-1.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg;IHC analysis of Gamma Catenin/JUP using anti-Gamma Catenin/JUP antibody (PA1117-1). <br>Gamma Catenin/JUP was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Gamma Catenin/JUP Antibody (PA1117-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1117-1-jup-primary-antibodies-wb-testing-1.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; Western blot analysis of JUP using anti-JUP antibody (PA1117-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human T47D whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUP antigen affinity purified polyclonal antibody (Catalog # PA1117-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JUP at approximately 82 kDa. The expected band size for JUP is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1117-1-jup-primary-antibodies-if-testing-2.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; IF analysis of JUP using anti-JUP antibody (PA1117-1). <br> JUP was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-JUP Antibody (PA1117-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1117-1-jup-primary-antibodies-fcm-testing-3.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-JUP antibody (PA1117-1). <br>Overlay histogram showing A431 cells stained with PA1117-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JUP Antibody (PA1117-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytochrome-c-antibody-pa1118-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (PA1118). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human HT1080 whole cell lysates, <br> Lane 5: rat kidney tissue lysates, <br> Lane 6: rat skeletal muscle tissue lysates, <br> Lane 7: mouse kidney tissue lysates, <br> Lane 8: mouse skeletal muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (PA1118) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-ihc-testing-6.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg;IHC analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (PA1118). <br>Cytochrome c/CYCS was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cytochrome c/CYCS Antibody (PA1118) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-5.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Cytochrome C was detected in paraffin-embedded section of human skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytochrome C Antibody (PA1118) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-6.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Cytochrome C was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytochrome C Antibody (PA1118) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-ihc-testing-3.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IHC analysis of Aspartate Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Aspartate Cytochrome C was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytochrome C Antibody (PA1118) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-if-testing-7.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IF analysis of CYCS using anti-CYCS antibody (PA1118). <br> CYCS was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CYCS Antibody (PA1118) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-ihc-testing-4.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IHC analysis of Aspartate Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Aspartate Cytochrome C was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytochrome C Antibody (PA1118) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-ihc-testing-5.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IHC analysis of Aspartate Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Aspartate Cytochrome C was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytochrome C Antibody (PA1118) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-ihc-testing-6.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IHC analysis of Aspartate Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Aspartate Cytochrome C was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytochrome C Antibody (PA1118) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-ihc-testing-8.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IHC analysis of Aspartate Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Aspartate Cytochrome C was detected in a paraffin-embedded section of human cervial tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytochrome C Antibody (PA1118) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-ihc-testing-7.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IHC analysis of Aspartate Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Aspartate Cytochrome C was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytochrome C Antibody (PA1118) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-if-testing-1_1.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IF analysis of Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Cytochrome C was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytochrome C Antibody (PA1118) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-if-testing-2.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IF analysis of Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Cytochrome C was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytochrome C Antibody (PA1118) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-if-testing-3.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IF analysis of Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Cytochrome C was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytochrome C Antibody (PA1118) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-if-testing-4.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IF analysis of Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Cytochrome C was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytochrome C Antibody (PA1118) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-if-testing-5.pngAnti-Cytochrome C/CYCS Antibody Picoband&reg;IF analysis of Cytochrome C using anti-Cytochrome C antibody (PA1118). <br> Cytochrome C was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytochrome C Antibody (PA1118) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1118-cycs-primary-antibodies-fcm-testing-8.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-CYCS antibody (PA1118). <br>Overlay histogram showing CACO-2 cells stained with PA1118 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYCS Antibody (PA1118, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fas-antibody-pa1119-boster.html2026-03-24T05:03:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1119-1-WB-anti-fas-cd95-antibody.jpgAnti-Fas Antibody Picoband&reg;Anti-Fas antibody&#44; PA1119&#44; Western blotting<br>Lane 1: Recombinant Mouse FAS Protein 10ng<br>Lane 2: Recombinant Mouse FAS Protein 5ng<br>Lane 3: Recombinant Mouse FAS Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1119-2-IHC-anti-fas-cd95-antibody.jpgAnti-Fas Antibody Picoband&reg;Anti-Fas antibody&#44; PA1119&#44; IHC(P)<br>IHC(P): Rat Spleen Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1119-3-WB-anti-fas-cd95-antibody.jpgAnti-Fas Antibody Picoband&reg;Western blot analysis of FAS expression in rat liver extract (lane 1)&#44; rat spleen extract (lane 2)&#44; rat brain extract (lane 3) and rat cardiac muscle extract (lane 4). FAS at 50KD was detected using rabbit anti-FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1119-4-WB-anti-fas-cd95-antibody.jpgAnti-Fas Antibody Picoband&reg;Western blot analysis of FAS expression in mouse liver extract (lane 1)&#44; mouse spleen extract (lane 2)&#44; mouse brain extract (lane 3) mouse kidney extract (lane 4)&#44; mouse thymus extract (lane 5)&#44; mouse lung extract (lane 6)&#44; HEPA1-6 whole cell lysates (lane 7) and NIH3T3 whole cell lysates (lane 8). FAS at 50KD was detected using rabbit anti-FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1119-41419_2017_article_bfcddis201791_fig6_html.jpgAnti-Fas Antibody Picoband&reg;Ovarian sections stained for FasL and Fas expression. Representative micrographs of antral follicles immunofluorescently stained for FasL ( a–f ) and Fas ( i–n ) expression in 17- and 40-week-old BRE +/+ , BRE +/− and BRE −/− ovaries. Higher-magnification images are highlighted at top right corners of c , f , k and n . ( g and h ) Bar charts showing areas that are FasL + over the total granulosa cell area of antral follicles, in 17- (G) and or 40- ( h ) week-old BRE +/+ , BRE +/− and BRE −/− ovaries. ( o and p ) Bar charts showing areas that are Fas + over the total granulosa cell area of antral follicles, in 17- ( o ) and or 40- ( p ) week-old BRE +/+ , BRE +/− and BRE −/− ovaries. AF, antral follicle; GC, granulosa cells. Scale bar=100 μ m in a – n <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis201791'>28333135</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1119-41419_2017_article_bfcddis201791_fig7_html.jpgAnti-Fas Antibody Picoband&reg;Ovarian sections stained for γ -H2AX. ( a–f ) Representative micrographs of 17- and 40-week-old BRE +/+ , BRE +/− and BRE −/− ovarian sections immunohistochemically stained for γ -H2AX (marker for DNA double-strand breaks). Higher-magnification image at the top right corner is taken from c and f . ( g and h ) Bar charts comparing the average number of γ -H2AX + granulosa cells in antral follicles of 17- ( g ) and 40- ( h ) week-old BRE +/+ , BRE +/− and BRE −/− ovaries. ( i ) Semiquantitative RT-PCR analysis showing ATM, PUMA, Fas and p53 expression in 40-week-old BRE +/+ and BRE −/− ovaries. The accompanying bar chart indicates the normalized and averaged expression of these genes. Scale bar=100 μ m in a – f <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis201791'>28333135</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1119-5.jpgAnti-Fas Antibody Picoband&reg; IHC analysis of FAS using anti-FAS antibody (PA1119). <br> FAS was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FAS Antibody (PA1119) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp16-antibody-pa1123-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1123-mmp16-primary-antibodies-wb-testing-1_1.jpgAnti-Matrix metalloproteinase-16 MMP16 Antibody Picoband&reg; Western blot analysis of MMP16 using anti-MMP16 antibody (PA1123). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP16 antigen affinity purified polyclonal antibody (Catalog # PA1123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP16 at approximately 70 kDa. The expected band size for MMP16 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1123-2-IHC-anti-mmp16-mt3-mmp-antibody.jpgAnti-Matrix metalloproteinase-16 MMP16 Antibody Picoband&reg; IHC analysis of MMP16 using anti-MMP16 antibody (PA1123).<br> MMP16 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1123-3.jpgAnti-Matrix metalloproteinase-16 MMP16 Antibody Picoband&reg; IHC analysis of MMP16 using anti-MMP16 antibody (PA1123). <br> MMP16 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1123-4.jpgAnti-Matrix metalloproteinase-16 MMP16 Antibody Picoband&reg; IHC analysis of MMP16 using anti-MMP16 antibody (PA1123). <br> MMP16 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP16 Antibody (PA1123) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nf-kb-p65-antibody-pa1125-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1125-1-WB-anti-nf-kb-p65-antibody.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Anti-NF-kB p65 antibody&#44; PA1125&#44; Western blotting<br>All lanes: Anti NF-kB p65 (PA1125) at 0.5ug/ml<br>Lane 1: Human Colon Cancer Tissue Lysate at 50ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 65KD<br>Observed bind size: 65KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-estrogen-receptor-beta-antibody-pa1126-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1126-1-WB-anti-estrogen-receptor-beta-antibody.jpgAnti-Estrogen Receptor beta/ESR2 Antibody Picoband&reg;Anti-Estrogen Receptor beta antibody&#44; PA1126&#44; Western blotting<br>WB: MCF-7 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-topoisomerase-ii-alpha-antibody-pa1127-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1127-1-WB-anti-topoisomerase-ii-alpha-antibody.jpgAnti-Topoisomerase II alpha/TOP2A Antibody Picoband&reg;Anti-Topoisomerase II alpha antibody&#44; PA1127&#44; Western blotting<br>All lanes: Anti Topoisomerase II alpha (PA1127) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 174KD<br>Observed bind size: 174KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hif-2-alpha-antibody-pa1129-2-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1129-2-wb_image.jpgAnti-HIF-2-alpha/EPAS1 Antibody Picoband&reg;<b> Western blot analysis of HIF2A using anti-HIF2A antibody (PA1129-2). </b><br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat thymus tissue lysates&#44; <br>Lane 2: rat testis tissue lysates&#44; <br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF2A antigen affinity purified polyclonal antibody (Catalog # PA1129-2) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF2A at approximately 110-120KD. The expected band size for HIF2A is at 96KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1129-2-2.jpgAnti-HIF-2-alpha/EPAS1 Antibody Picoband&reg; Western blot analysis of HIF-2A using anti-HIF-2A antibody (PA1129-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates&#44; <br> Lane 2: human Hela whole cell lysates&#44; <br> Lane 3: human Jurkat whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody (Catalog # PA1129-2) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1129-2-3.jpgAnti-HIF-2-alpha/EPAS1 Antibody Picoband&reg; Western blot analysis of HIF-2A using anti-HIF-2A antibody (PA1129-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse thymus tissue lysates&#44; <br> Lane 2: mouse lun tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody (Catalog # PA1129-2) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1129-2-4_.jpgAnti-HIF-2-alpha/EPAS1 Antibody Picoband&reg; Western blot analysis of HIF-2A using anti-HIF-2A antibody (PA1129-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat RH35 whole cell lysates&#44; <br> Lane 3: rat small intestine tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2A antigen affinity purified polyclonal antibody (Catalog # PA1129-2) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-2A at approximately 120KD. The expected band size for HIF-2A is at 96KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1129-2-epas1-primary-antibodies-wb-testing-5.jpgAnti-HIF-2-alpha/EPAS1 Antibody Picoband&reg; Western blot analysis of EPAS1 using anti-HIF-2-alpha/EPAS1 antibody (PA1129-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk for 2 hour at RT. The membrane was incubated with rabbit anti-HIF-2-alpha/EPAS1 antibody (PA1129-2) at 1:1000 in 5% milk at 4℃ rotating , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat-anti-mouse HRP secondary antibody at a dilution of 1:2000 for 2 hour rotating at RT. The signal is developed using a SuperSignal West Femto Maximum Sensitivity Substrate. A specific band was detected for EPAS1 at approximately 120 kDa. The expected band size for EPAS1 is at 120 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr6-antibody-pa1201-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1201-1-WB-anti-ccr6-antibody.jpgAnti-CCR6/CCR6 Antibody Picoband&reg;Anti-CCR6 antibody&#44; PA1201&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: SW620 Cell Lysate<br>Lane 3: CEM Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-chk2-antibody-pa1202-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1202-1_1-WB-anti-chk2-antibody.jpgAnti-Chk2/CHEK2 Antibody Picoband&reg; Western blot analysis of Chk2 using anti-Chk2 antibody (PA1202). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human MDA-MB-231 whole cell lysates&#44; <br> Lane 2: human MDA-MB-453 whole cell lysates&#44; <br> Lane 3: human HepG2 whole cell lysates&#44; <br> Lane 4: human K562 whole cell lysates&#44;<br> Lane 5: human Caco-2 whole cell lysates&#44;<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk2 antigen affinity purified polyclonal antibody (Catalog # PA1202) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk2 at approximately 69KD. The expected band size for Chk2 is at 61KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nicotinic-acetylcholine-receptor-alpha-1-antibody-pa1203-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1203-1-WB-anti-nicotinic-acetylcholine-receptor-alpha-1-antibody.jpgAnti-Nicotinic Acetylcholine Receptor alpha 1/CHRNA1 Antibody Picoband&reg;Anti-Nicotinic Acetylcholine Receptor alpha 1 antibody&#44; PA1203&#44; Western blotting<br>WB: Rat Skeletal Muscle Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hrh3-antibody-pa1204-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1204-1_1.jpgAnti-Histamine H3 receptor HRH3 Antibody Picoband&reg; Western blot analysis of HRH3 using anti-HRH3 antibody (PA1204). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U-87MG whole cell lysates<br> Lane 2: human SHG-44 whole cell lysates<br> Lane 3: mouse RAW246.7 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRH3 antigen affinity purified polyclonal antibody (Catalog # PA1204) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HRH3 at approximately 49KD. The expected band size for HRH3 is at 49KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mrgx1-antibody-pa1208-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1208-1-WB-anti-mrgx1-antibody.jpgAnti-MRGX1/MRGPRX1 Antibody Picoband&reg;Anti-MRGX1 antibody&#44; PA1208&#44; Western blotting<br>Lane 1: Marker<br>Lane 2: Rat Heart Tissue Lysate<br>Lane 3: Rat Skeletal Muscle Tissue Lysate<br>Lane 4: MCF-7 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-receptor-i-antibody-pa1210-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1210-2-IHC-anti-tnfsr-i-antibody.jpgAnti-TNF Receptor I/TNFRSF1A Antibody Picoband&reg;Anti-TNF Receptor I antibody&#44; PA1210&#44; IHC(P)<br>IHC(P): Human Mammary Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1210-1_1.jpgAnti-TNF Receptor I/TNFRSF1A Antibody Picoband&reg; Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (PA1210).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: human SW579 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # PA1210) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50-60KD. The expected band size for TNF Receptor I is at 50KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-catenin-antibody-pa1212-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1212-1-WB-anti-beta-catenin-antibody.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;Anti-beta Catenin antibody&#44; PA1212&#44; Western blotting<br>Lane 1: MM453 Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1212-2-IHC-anti-beta-catenin-antibody.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;Anti-beta Catenin antibody&#44; PA1212&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1212-3-IHC-anti-beta-catenin-antibody.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;Anti-beta Catenin antibody&#44; PA1212&#44; IHC(F)<br>IHC(F): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1212-41598_2024_63055_fig4_html.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;TOP2A is involved in the progression and development of NSCLC via the Wnt/β-catenin signaling cascade. ( A ) TCGA enrichment analysis revealed that TOP2A expression is enriched in the Wnt signaling pathway (n = 1037). ( B ) Several NSCLC cell lines exhibited a clear association between the transcription of TOP2A and β-catenin. ( C ) The online GEPIA exhibited a correlation between TOP2A and WNT3A gene expression levels. ( D,E ) TOP2A overexpression and knockdown groups were examined for gene and protein levels of TOP2A, Wnt3a, and β-catenin. ( F ) Immunohistochemical assessment of Wnt3a and β-catenin expression in the TOP2A-positive and TOP2A-negative groups (200×). ( G ) Patients with high Wnt3a overexpression had an abysmal survival rate. *P < 0.05, **P < 0.01, or ***P < 0.001. All experiments were repeated three times independently.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11133405/&orig_host=www.ncbi.nlm.nih.gov'>38806610</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1212-41598_2024_63055_fig5_html.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;TOP2A promotes non-small cell lung cancer progression via Wnt/β-catenin signaling pathway. ( A ) After Wnt3a gene knockdown, the mobility and invading capabilities of TOP2A-overexpressing cells were evaluated using the transwell assay. ( B,C ) Expression of components of Wnt/β-catenin signaling pathway and EMT-related proteins was examined using western blotting. ( D ) Flow cytometry was utilized to examine the cell cycle. ( E ) Analysis of apoptosis via flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001 or ****P < 0.0001; ns: not signiffcant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11133405/&orig_host=www.ncbi.nlm.nih.gov'>38806610</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1212_13020_2025_1171_fig7_html.pngAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;SM impedes traits of CSCs partially through suppressing β-catenin in CRC. A Western blotting, B ALDH + staining and C tumorsphere formation assay were performed after CRC cells transduced with lentiviral β-catenin (encoded by the CTNNB1 gene) cDNA-expressing construct in the presence or absence of SM. Scale bar: 100 μm. D Western blotting, E ALDH + staining and F tumorsphere formation assays were conducted after CRC cells stably transduced with lentiviral shRNA against β-catenin with or without SM treatment. Scale bar: 100 μm. The values are represented as mean ± SD. * p < 0.05, *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01171-5'>40646619</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1212_13020_2025_1171_fig8_html.pngAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;SM inhibits hepatic metastasis of CRC cells partially by attenuating β-catenin. A Transwell migration and B matrigel invasion assays were conducted followed by CRC cells transduced with lentiviral β-catenin-encoding construct with or without SM treatment. Scale bar: 100 μm. C Transwell migration and D matrigel invasion assays were conducted followed by CRC cells transduced with lentiviral shRNA against β-catenin in the presence or absence of SM. Scale bar: 100 μm. E The overexpression efficiency of β-catenin in MC38-Luc cells was examined by Western blotting analysis. F Representative images and G quantitative analysis of photon flux on day 21 of C57BL/6 mice followed by intrasplenic injection of MC38-Luc cells stably express β-catenin. n = 5 per group. H Representative images and quantitative analysis of liver metastasis in C57BL/6 mice (n = 5) measured by bioluminescence imaging. I Representative images of metastatic livers and ( J ) the number of surface foci in the livers in C57BL/6 mice (n = 5) are shown. Arrows indicate the presence of metastatic nodules. K Representative photographs of H&E staining of the liver section and L the number of liver metastatic foci in microscopic fields are presented (n = 3). Scale bar: 200 μm. The values are represented as mean ± SD. * p < 0.05, *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01171-5'>40646619</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1212-beta-catenin-primary-antibodies-if-testing-4.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg; IF analysis of Beta Catenin using anti-Beta Catenin antibody (PA1212). <br> Beta Catenin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Beta Catenin Antibody (PA1212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-catenin-antibody-pa1212-1-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1212-1-1-WB-anti-beta-catenin-antibody.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;Anti-beta Catenin antibody&#44; PA1212-1&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Heart Tissue Lysate<br>Lane 3: Rat Testis Tissue Lysate<br>Lane 4: MCF-7 Cell Lysate<br>Lane 5: HELA Cell Lysate<br>Lane 6: M453 Cell Lysate<br>Lane 7: M231 Cell Lysate<br> Lane 8: HT1080 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1212-1-2-IHC-anti-beta-catenin-antibody.jpgAnti-beta Catenin/CTNNB1 Antibody Picoband&reg;Anti-beta Catenin antibody&#44; PA1212-1&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp70-antibody-pa1214-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-1_1.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; Western blot analysis of Hsp70 using anti-Hsp70 antibody (PA1214). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates&#44; <br> Lane 2: human Caco-2 whole cell lysates&#44; <br> Lane 3: human PC-3 whole cell lysates&#44; <br> Lane 4: human THP-1 whole cell lysates&#44; <br> Lane 5: human U2OS whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antigen affinity purified polyclonal antibody (Catalog # PA1214) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp70 at approximately 70KD. The expected band size for Hsp70 is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-hsp70-primary-antibodies-ihc-testing-6.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg;IHC analysis of HSP70 using anti-HSP70 antibody (PA1214). <br>HSP70 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSP70 Antibody (PA1214) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-41598_2025_95332_fig3_html.pngAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg;Interactions between 2-undecanone and the target proteins Hsp70 A and V-ATPase A. ( A ) Fluorescence counts of Hsp70 A-RED protein. ( B ) Dose-response curve for the binding interaction between Hsp70 A and 2-undecanone by using MST analysis. ( C ) Fluorescence counts of V-ATPase-A-RED protein. ( D ) Dose-response curves for the binding interaction between V-ATPase-A and 2-undecanone by using MST analysis. ( E ) Sensitivity of hsp-1 , vha-13 or hsp-1 / vha-13 RNAi worms to 2-undecanone. Data represents means ± SD from triplicate experiments. * P < 0.05, ** P < 0.01 ( F ) hsp-1 , vha-13 or hsp-1 / vha-13 RNAi worms stained by intestinal lysosomal marker Lyso-Tracker. N = 6, scale bars, 20 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-95332-z'>40269070</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-fonc-10-537322-g005.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg;Label-free proteomic and bioinformatic analysis of proteins associated with the cell cycle, apoptosis, and autophagy. (A–C) The DAVID database was used for analysis of molecular function, cellular component, and biological process. (D) A heat map was constructed based on the abundance of 111 proteins. D1, D2, and D3 represent the DMSO 1, DMSO 2, and DMSO 3 groups; G1, G2, and G3 represent the germacrone 1, germacrone 2, and germacrone 3 groups. (E) A protein interaction network was constructed in Cytoscape based on the information provided by the STRING database. Red indicates increased expression of the protein in the germacrone group; green indicates reduced expression of the protein in the germacrone group. (F, G) . The expression of HBXIP, HSP70, and ANXA1 was detected to verify the accuracy of the proteomics results.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.537322/full'>33244453</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-13046_2019_1437_fig5_html.pngAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg;Exosomal circWHSC1 promotes peritoneal dissemination and regulates expression of MUC1 in peritoneum. Exosome specific markers, CD63, CD9, HSP70 and TSG101 were expressed in the extracted exosome pellets ( a ). Electron microscopy confirmed diameters of the extracted exosomes ranged from 10 to 100 nm and with cup shape appearance ( b ). The morphology of HMrSV5 cells incubated with exosomal circWHSC1 was converted into fibroblast-like ( c ). N-cadherin and MUC1 were up-regulated, while E-cadherin was down-regulated in exosome-treated HMrSV5 cells ( d ). After injection with circWHSC1 exosomes, the number of tumor nodules was significantly increased in abdominal cavity ( e ). HE staining showed that the peritoneum was covered with a monolayer of mesothelial cells with intact intercellular junctions from two different perspectives ( f ). After the treatment of exosomal circWHSC1, mesothelial cells arranged loosely, and the stromal layer was reactively thicken surrounding infiltration of tumor cells, and the left and right graphs represent two different perspectives ( g ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1437-z'>31666098</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1214-2-IHC-anti-hsc70-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). <br> Hsp70 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1214-3-IHC-anti-hsc70-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). <br> Hsp70 was detected in paraffin-embedded section of mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1214-4-IF-anti-hsc70-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). <br> Hsp70 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1214-5-IHC-anti-hsc70-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IHC analysis of Hsp70 using anti-Hsp70 antibody (PA1214). <br> Hsp70 was detected in frozen section of rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-6.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; Western blot analysis of Hsp70 using anti-Hsp70 antibody (PA1214). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat liver tissue lysates&#44; <br> Lane 3: rat spleen tissue lysates&#44; <br> Lane 4: rat PC-12 whole cell lysates&#44; <br> Lane 5: mouse brain tissue lysates&#44; <br> Lane 6: mouse liver tissue lysates&#44; <br> Lane 7: mouse spleen tissue lysates&#44; <br> Lane 8: mouse RAW246.7 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antigen affinity purified polyclonal antibody (Catalog # PA1214) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp70 at approximately 70KD. The expected band size for Hsp70 is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-7.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IF analysis of Hsp70 using anti-Hsp70 antibody (PA1214). <br> Hsp70 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hsp70 Antibody (PA1214) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1214-8.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-Hsp70 antibody (PA1214).<br>Overlay histogram showing 293T cells stained with PA1214 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp70 Antibody (PA1214,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-notch1-antibody-pa1215-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1215-1_1-WB-anti-notch1-antibody.jpgAnti-Notch1 Antibody Picoband&reg;Anti-Notch1 antibody&#44; PA1215&#44; Western blotting<br>All lanes: Anti Notch1 (PA1215) at 0.5ug/ml<br>Lane 1: MCF-7 Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Lane 4: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 272KD<br>Observed bind size: 272KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf8-antibody-pa1216-boster.html2026-03-24T05:03:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1216-1-WB-anti-fgf8-antibody.jpgAnti-Fibroblast growth factor 8 FGF8 Antibody Picoband&reg;Anti-FGF8 antibody&#44; PA1216&#44; Western blotting<br>WB: Rat Ovary Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1216-2-IHC-anti-fgf8-antibody.jpgAnti-Fibroblast growth factor 8 FGF8 Antibody Picoband&reg;Anti-FGF8 antibody&#44; PA1216&#44; IHC(P)<br>IHC(P): Rat Ovary Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1216-3.jpgAnti-Fibroblast growth factor 8 FGF8 Antibody Picoband&reg; IHC analysis of FGF8 using anti-FGF8 antibody (PA1216). <br> FGF8 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FGF8 Antibody (PA1216) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xaf1-antibody-pa1218-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1218-xaf1-primary-antibodies-wb-testing-1_1.jpgAnti-XIAP-associated factor 1 XAF1 Antibody Picoband&reg; Western blot analysis of XAF1 using anti-XAF1 antibody (PA1218). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XAF1 antigen affinity purified polyclonal antibody (PA1218) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for XAF1 at approximately 35 kDa. The expected band size for XAF1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1218-xaf1-primary-antibodies-if-testing-2.jpgAnti-XIAP-associated factor 1 XAF1 Antibody Picoband&reg; IF analysis of XAF1 using anti-XAF1 antibody (PA1218). <br> XAF1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-XAF1 Antibody (PA1218) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1218-xaf1-primary-antibodies-fcm-testing-3.jpgAnti-XIAP-associated factor 1 XAF1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-XAF1 antibody (PA1218). <br> Overlay histogram showing SiHa cells stained with PA1218 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XAF1 Antibody (PA1218, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmdar1-antibody-pa1222-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1222-1-WB-anti-nmdar1-antibody.jpgAnti-NMDAR1/GRIN1 Antibody Picoband&reg;Anti-NMDAR1 antibody&#44; PA1222&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: Rat Liver Tissue Lysate<br>Lane 4: Rat Heart Tissue Lysate<br>Lane 5: MM453 Cell Lysate<br>Lane 6: MM231 Cell Lysate<br>Lane 7: HELA Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1222-2-IHC-anti-nmdar1-antibody.jpgAnti-NMDAR1/GRIN1 Antibody Picoband&reg;Anti-NMDAR1 antibody&#44; PA1222&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1222-grin1-primary-antibodies-ihc-testing-3.jpgAnti-NMDAR1/GRIN1 Antibody Picoband&reg;IHC analysis of GRIN1 using anti-GRIN1 antibody (PA1222). <br>GRIN1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRIN1 Antibody (PA1222) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-runx2-antibody-pa1224-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1224-1_1.jpgAnti-RUNX2 Antibody Picoband&reg; Western blot analysis of RUNX2 using anti-RUNX2 antibody (PA1224). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U-87MG whole cell lysate&#44; <br> Lane 2: rat liver tissue lysate&#44; <br> Lane 3: mouse liver tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PA1224) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 62KD. The expected band size for RUNX2 is at 58KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-liver-fabp-antibody-pa1229-1-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1229-1-1-WB-anti-liver-fabp-antibody.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg;Anti-liver FABP antibody&#44; PA1229-1&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Rat Kidney Tissue Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: NEURO Cell Lysate<br>Lane 5: SMMC Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1229-1-2-IHC-anti-liver-fabp-antibody.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg;Anti-liver FABP antibody&#44; PA1229-1&#44; IHC(P)<br>IHC(P): Rat Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1229-1-3-IHC-anti-liver-fabp-antibody.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg;Anti-liver FABP antibody&#44; PA1229-1&#44; IHC(P)<br>IHC(P): Human Liver Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-5-antibody-pa1230-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1230-1-WB-anti-aquaporin-5-antibody.jpgAnti-Aquaporin 5/AQP5 Antibody Picoband&reg;Anti-Aquaporin 5 antibody&#44; PA1230&#44; Western blotting<br>Working concentration: 0.5μg/ml<br>Lane 1: Rat Lung Tissue Lysate<br>Lane 2: Rat Testis Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1230-2-IHC-anti-aquaporin-5-antibody.jpgAnti-Aquaporin 5/AQP5 Antibody Picoband&reg;Anti-Aquaporin 5 antibody&#44; PA1230&#44; IHC(P)<br>IHC(P): Rat Lung Tissue Lysate<br>Working concentration: 1μg/ml https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcl-xs-antibody-pa1232-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1232-bcl2l1-primary-antibodies-wb-testing-1.jpgAnti-Bcl-XS/BCL2L1 Antibody Picoband&reg;Western blot analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XS/BCL2L1 antigen affinity purified polyclonal antibody (PA1232) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Bcl-XS/BCL2L1 at approximately 18-20 kDa. The expected band size for Bcl-XS/BCL2L1 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1232-bcl2l1-primary-antibodies-wb-testing-2.jpgAnti-Bcl-XS/BCL2L1 Antibody Picoband&reg;Western blot analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat pancrease tissue lysates, Lane 2: mouse pancrease tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-XS/BCL2L1 antigen affinity purified polyclonal antibody (PA1232) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Bcl-XS/BCL2L1 at approximately 18-20 kDa. The expected band size for Bcl-XS/BCL2L1 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1232-2-IHC-anti-bcl-xs-antibody.jpgAnti-Bcl-XS/BCL2L1 Antibody Picoband&reg;Anti-Bcl-XS antibody&#44; PA1232&#44; IHC(P)<br>IHC(P): Human Colon Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1232-bcl2l1-primary-antibodies-ihc-testing-3.jpgAnti-Bcl-XS/BCL2L1 Antibody Picoband&reg;IHC analysis of Bcl-XS/BCL2L1 using anti-Bcl-XS/BCL2L1 antibody (PA1232). <br>Bcl-XS/BCL2L1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bcl-XS/BCL2L1 Antibody (PA1232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cullin-4b-antibody-pa1233-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1233-2-IHC-anti-cullin-4b-antibody.jpgAnti-Cullin 4B/CUL4B Antibody Picoband&reg;Anti-Cullin 4B antibody&#44; PA1233&#44; IHC(P)<br>IHC(P): Zebrafish Body Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1233-1_1.jpgAnti-Cullin 4B/CUL4B Antibody Picoband&reg; Western blot analysis of Cullin 4B using anti-Cullin 4B antibody (PA1233). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysate&#44; <br> Lane 2: human A549 whole cell lysate&#44; <br> Lane 3: rat thymus tissue lysate&#44; <br> Lane 4: rat kidney tissue lysate&#44; <br> Lane 5: mouse kidney tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4B antigen affinity purified polyclonal antibody (Catalog # PA1233) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4B at approximately 104KD. The expected band size for Cullin 4B is at 104KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-gamma-antibody-pa1234-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1234-1-WB-anti-pkc-gamma-antibody.jpgAnti-PKC gamma/PRKCG Antibody Picoband&reg;Anti-PKC gamma antibody&#44; PA1234&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ssx2-antibody-pa1235-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1235-1-WB-anti-ssx2-antibody.jpgAnti-Protein SSX2 SSX2 Antibody Picoband&reg;Anti-SSX2 antibody&#44; PA1235&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: HELA Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1235-2-IHC-anti-ssx2-antibody.jpgAnti-Protein SSX2 SSX2 Antibody Picoband&reg;Anti-SSX2 antibody&#44; PA1235&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcr4-antibody-pa1237-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1237-1_1.jpgAnti-CXCR4 Antibody Picoband&reg; Western blot analysis of CXCR4 using anti-CXCR4 antibody (PA1237). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates&#44; <br> Lane 2: human Hela whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR4 antigen affinity purified polyclonal antibody (Catalog # PA1237) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR4 at approximately 40-43KD. The expected band size for CXCR4 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1237-oncotarget-08-63461-g002.jpgAnti-CXCR4 Antibody Picoband&reg;Panc02-H7-derived exosomes promote metastatis-related characteristics in vitro . Panc02 cells tookup PKH67-labeled Panc02-H7EXOs. Numerous green fluorescently-labeled exosomes were observed inside cells after 5 h (400× magnification). (A) The MTT cell adhesion assay indicated that Panc02-H7 EXOs decrease Panc02 cell adhesion. (B) Wound-healing assays indicated that Panc02-H7 EXOs enhanced Panc02 cell migration (200×magnification). (C) Transwell chamber invasion assays showed that Panc02-H7 EXOs increased Panc02 cell invasion (200×magnification). (D) Western blotting indicated that Panc02-H7 EXOs increased Panc02 cell migration and invasion via CXCR4 and MMP-9 signaling. (E) n=3/group.*P<0.05,**P<0.01, ***P<0.001 compared to control; #P<0.05, ##P<0.01, ###P<0.001 compared to EXO-D.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5609937/&orig_host=www.ncbi.nlm.nih.gov'>28969005</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1237-2-IHC-anti-cxcr4-antibody.jpgAnti-CXCR4 Antibody Picoband&reg; IHC analysis of CXCR4 using anti-CXCR4 antibody (PA1237). <br> CXCR4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CXCR4 Antibody (PA1237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gfap-antibody-pa1239-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-gfap-primary-antibodies-wb-testing-1.jpgAnti-GFAP Antibody Picoband&reg; Western blot analysis of GFAP using anti-GFAP antibody (PA1239). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (Catalog # PA1239) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-gfap-primary-antibodies-ihc-testing-5.jpgAnti-GFAP Antibody Picoband&reg;IHC analysis of GFAP using anti-GFAP antibody (PA1239). <br>GFAP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-fphar-16-1554945-g007.jpgAnti-GFAP Antibody Picoband&reg;TUDCA regulated monocytes distribution and impacted glial scar formation. Co-immunofluorescence images showed reactive astrocytes (GFAP, green) and monocytes (CD11b, red) at day 3 and day 7 after SCI.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-fphar-16-1554945-g009.jpgAnti-GFAP Antibody Picoband&reg;TUDCA regulated macrophages and reactive astrocytes distribution. Co-immunofluorescence images showed macrophages (CD68, red) and reactive astrocytes (GFAP, green) at day 3 and day 7 after SCI.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-fphar-16-1554945-g011.jpgAnti-GFAP Antibody Picoband&reg;TUDCA promoted neuron regeneration along endogenous NSCs migration at day 7 after SCI. (A) Co-immunofluorescence showed endogenous NSCs (Nestin, green) and reactive astrocytes (GFAP, red) at the margin of the lesion site at day 7 after SCI. (B) Endogenous NSCs (Nestin, green) and neuron (NeuN, red) at the margin of the lesion site at day 7 after SCI. (C, D) Quantitative polymerase chain reaction (qPCR) showing the expression of Nestin, GFAP, NeuN, MAP2 and Oligo 2 at day 7 after SCI. All experiments were performed in triplicated and data were presented means ± SEM, n = 3 per group. *P < 0.05, **P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1554945/full'>40276612</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12974_2025_3406_fig6_html.pngAnti-GFAP Antibody Picoband&reg;ErbB4 small molecule agonist can improve hippocampal neuroinflammation in mice exposed to PS-MPs via the TLR4/NLRP3 pathway. ( A ) Levels of IBA1 were detected through immunofluorescence staining. ( B ) Levels of GFAP were detected through immunofluorescence staining. ( C ) Western blot analysis was utilized to detect protein levels associated with the TLR4/NLRP3 signaling pathway in the hippocampus of mice. ( D ) Statistical chart was generated to display the protein levels of TLR4/NLRP3. GFAP and IBA1 are both indicated in red, with a scale bar of 20 μm. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons, and the data are reported as means ± SEM, with significance levels denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001. Each group comprised n = 4 samples <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-025-03406-6'>40089796</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12974_2024_3084_fig3_html.pngAnti-GFAP Antibody Picoband&reg;NSA or Mdivi-1 attenuated the activation of microglia and astrocytes and the release of inflammatory cytokines in the hippocampus of SAE mice. A Representative images of Iba-1 (green) in the hippocampal CA1 region. B Quantification of Iba-1 fluorescence hippocampal CA1 region. C Representative images of GFAP (red) in the hippocampal CA1 region. D Quantification of GFAP fluorescence hippocampal CA1 region. Data are presented as mean ± SEM ( n = 4–6 mice/group). DAPI staining is shown in blue. Scale bar = 50 μm. E – G Representative western blotting and quantitative analysis of the protein levels of IL-1β, IL-18 and TNF-a in the hippocampus. Data are presented as the mean ± SEM ( n = 4 mice/group). * P < 0.05, ** P < 0.01 versus the indicated groups <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-024-03084-w'>38627764</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-fphar-14-1188893-g004.jpgAnti-GFAP Antibody Picoband&reg;LWD reduced the Aβ and inflammatory response in the hippocampus of the APP/PS1 mice and the activated number of glial cells. (A) Positive expression of Aβ in each group in immunofluorescence. (B) Quantitative histogram of Aβ positive expression in each group. (C) Positive expression of IBA1 in each group in immunofluorescence. (D) Quantitative histogram of IBA1 positive expression in each group. (E) Positive expression of GFAP in each group in immunofluorescence. (F) Quantitative histogram of GFAP positive expression in each group. (G–I) Protein expression and quantitative histogram of pro-inflammatory factors IL-1β and IL-10 in each group. ( n = 3, compared with the WT group, # p < 0.05, ## p < 0.01, ### p < 0.001; compared with the APP/PS1 group, * p < 0.05, ** p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10619154/&orig_host=www.ncbi.nlm.nih.gov'>37920210</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-40824_2023_339_fig6_html.pngAnti-GFAP Antibody Picoband&reg;EX-Netrin1 improved functional recovery and reduced histopathological injury in SCI rats. ( A ) MRI was used to detect spinal cord injury in rats after 28 days. The red dotted box indicated the site of spinal cord injury. ( B ) BBB score was used to evaluate the functional recovery of hind limbs in rats. ( C ) After 28 days, spinal cord tissue was collected and ELISA was performed to detect the contents of TNF-α, IL-1β and IL-6 in spinal cord. ( D ) HE and Nissl staining of spinal cord tissues. Red arrows indicated Nissl bodies. Scale bar: 50 μm. ( E - F ) Representative images showing neurofilaments (NFs, green) and glial fibrillary acidic protein (GFAP, red) staining of spinal cord tissues, and (F) the density ratios of NFs. (Error bars showed means ± SD; n = 8. *P < 0.05, **P < 0.01, ***P < 0.001, vs. Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. Model+PBS group; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001, vs. Model+EX-MSCs group; $ P < 0.05, $$ P < 0.01, $$$ P < 0.001, vs. Model+EX-NC group) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40824-023-00339-0'>36647161</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41467_2022_34662_fig1_html.pngAnti-GFAP Antibody Picoband&reg;Optogenetic stimulation of ChR2-expressing astrocytes attenuates KA-induced neocortical seizures. a Schematic of viral injection, stimulation and EEG recording in GFAP-ChR2 M1 mice. b Fluorescent image showed restricted expression of ChR2-EYFP in M1. Scale bar, 200 μm. c Left, fluorescent image of ChR2 (green) showed co-localization with GFAP + astrocytes (red). Right, fluorescent image of ChR2 (green) showed no co-localization with NeuN + neurons (red). Scale bar, 20 μm. d Light pulses induced inward currents in ChR2-expressing astrocyte in a cortical slice. Voltage responses from a ChR2-expressing astrocyte evoked by 10-pA step current from 0 to 300 pA. e Paradigms of blue light stimulation in different phases of KA-induced seizures. f Effects of optogenetic stimulation of astrocytes on the development of seizure stage during 90 min after KA injection. g – j Effects of different-phase optogenetic stimulation of astrocytes on seizure stage ( g ), EEG onset ( h ), latency to GS ( i ) and number of GSs ( j ) in KA-induced seizures. k Representative EEGs and corresponding energy spectra recorded from the M1 during KA-induced seizures. l Schematic of viral injection (LM1), stimulation (LM1) and EEG recording (RM1) in GFAP-ChR2 M1 mice. m Effects of optogenetic stimulation of LM1 astrocytes on the development of seizure stage during 90 min after KA injection. n – q Effects of optogenetic stimulation of LM1 astrocytes on seizure stage ( n ), EEG onset ( o ), latency to GS ( p ) and number of GSs ( q ) after RM1 KA injection. ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared with EYFP control. Data shown as mean ± s.e.m. The number of mice used is indicated in figures. For detailed statistical information, see Supplementary Data . Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-34662-2'>36414629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12886_2022_2592_fig1_html.pngAnti-GFAP Antibody Picoband&reg;Cell identification results: The results of cell identification showed these cells were astrocytes through positive staining for GFAP, vimentin and Connexin43(CX43). Negative staining for Aquaporin 4 (AQP4), OSP and F4/80 was also shown. Scale bar: 100 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12886-022-02592-8'>36114477</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41467_2022_34662_fig2_html.pngAnti-GFAP Antibody Picoband&reg;Optogenetic stimulation of ChR2-expressing astrocytes activity-dependently inhibits firing of pyramidal neurons. a Schematic of in vivo single-unit recording in naïve GFAP-ChR2 M1 mice. Astrocyte (green), Neuron (red). b The online recording process and offline sorting process of in vivo single-unit recording of putative pyramidal neuron with wide waveform. Inset panel, corresponding protrude autocorrelation of a representative putative pyramidal neuron. c Summary data of the responses of recorded M1 pyramidal neurons during astrocyte stimulation. d – f Representative peri-event raster histograms and time-frequency statistical charts showed the response of blue light stimulation in different frequency groups. g Schematic of in vivo single-unit recording in GFAP-ChR2 M1 mice in KA-induced seizure status. h Left panel, representative spikes and local field potentials of M1 pyramidal neurons during interictal and seizure phases. Right panel, quantification of the frequency of M1 pyramidal neurons during interictal and seizure phases. i Summary data of the responses of recorded M1 pyramidal neurons during astrocyte stimulation in KA-induced seizure status. j Time-frequency statistical charts showed the response of blue-light stimulation in different frequency groups in KA-induced seizure status. k Schematic of in vivo single-unit recording in RM1 after RM1 KA injection and LM1 blue light stimulation. l Summary data of the responses of recorded RM1 pyramidal neurons during astrocytes stimulation after RM1 KA injection and LM1 blue light stimulation. m , n Time-frequency statistical charts showed the response of blue-light stimulation in different frequency groups in KA-induced seizure status. ** p < 0.01 compared with interictal phase. Data shown as mean ± s.e.m. The number of mice used is indicated in figures. For detailed statistical information, see Supplementary Data . Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-34662-2'>36414629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12886_2022_2592_fig7_html.pngAnti-GFAP Antibody Picoband&reg;Western blot analysis of specific protein expression ( n = 3). ( A ) Colored bands of GFAP, LRP6, THBS1 and CD81; ( B ) Expression levels of LRP6, THBS1 and CD81 gradually decreased with the increase of the stretch amplitude, while the expression level of GFAP gradually increased with the increase of the stretch amplitude. Error bars indicate standard error of the mean (SEM); * P < 0.05 and ** P < 0.01 indicate statistical significance <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12886-022-02592-8'>36114477</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41467_2022_34662_fig4_html.pngAnti-GFAP Antibody Picoband&reg;Astrocytic calcium signaling is not required to produce the anti-seizure effects induced by optogenetic stimulation of astrocytes. a GFAP-Cre mice were injected with Cre-dependent virus expressing GCAMP6m-GFP or ChR2-EYFP in the M1 and implanted with cannula and electrode for drug injection, blue light stimulation and EEG recording. b Typical calcium signals and corresponding seizure EEGs in KA or KA + TG/BAPTA-AM injection groups. Δ F/F represents the change in signal amplitude from the base level. c Effects of optogenetic stimulation of astrocytes on the development of seizure stage, in the condition of calcium signaling inhibition by TG or BAPTA-AM injection. d – g Effects of optogenetic stimulation of astrocytes on seizure stage ( d ), EEG onset ( e ), latency to GS ( f ) and number of GSs ( g ), in the condition of calcium signaling inhibition by TG or BAPTA-AM injection. h GFAP-Cre mice were injected with Cre-dependent virus expressing GCAMP6m-GFP and/or hM3D(Gq)-mCherry in the M1 and implanted with cannula and electrode for KA injection and EEG recording. i Typical calcium signals before and after CNO injection indicating hM3Dq-mediated increase of calcium signaling. Δ F/F represents the change in signal amplitude from the base level. j Effects of chemogenetic activation of astrocytes on the development of seizure stage during 90 min after KA injection. k – n Effects of chemogenetic activation of astrocytes on the seizure stage ( k ), EEG onset ( l ), latency to GS ( m ) and number of GSs ( n ) in different groups. ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared with each control group. l * p < 0.05 compared with mCherry-CNO group, # p < 0.05 compared with hM3Dq-Saline group. Data shown as mean ± s.e.m. The number of mice used is indicated in figures. For detailed statistical information, see Supplementary Data . Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-34662-2'>36414629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-13020_2022_639_fig6_html.pngAnti-GFAP Antibody Picoband&reg;BSHX decoction reduced reactive astrocytosis after SCI. A Immunofluorescence images showed the reactive astrocytosis (GFAP, red) at day 3 and 7 after SCI. B Immunofluorescence images showed the neural stem cell (Nestin, green) in the lesion epicenter at day 3 and 7 after SCI. C Quantitative polymerase chain reaction (qPCR) showing the expression of GFAP after SCI. D Quantification of Nestin positive cells in the lesion epicenter. All experiments were performed in triplicated and data were presented as means ± SD, n = 3 per group. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-022-00639-y'>35820953</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41467_2022_34662_fig5_html.pngAnti-GFAP Antibody Picoband&reg;Astrocytic Na + -K + -ATPase mediates the anti-seizure effect of optogenetic stimulation of astrocytes. a GFAP-Cre mice were injected with Cre-dependent ChR2-mCherry and/or pAAV-shortGFAP-MCS-EGFP-3FLAG-mir30shRNA(Atp1α2) in the M1 and implanted with cannula and electrode for KA injection, blue light stimulation and EEG recording. b Fluorescent images showed that shRNA (green) showed co-localization with GFAP + astrocytes (red), and ATP1A2 protein co-localization with GFAP + shRNA(NC) + astrocytes from control shRNA(NC) injected cortical tissue. ATP1A2 protein expression in the GFAP + astrocytes is significantly reduced in shRNA(Atp1α2) injected cortical tissue. Scale bar, 20 μm. c Effects of optogenetic stimulation of astrocytes on the development of seizure stage, in the condition of astrocytic Na + -K + -ATPase inhibition. d – g Effects of optogenetic stimulation of astrocytes on seizure stage ( d ), EEG onset ( e ), latency to GS ( f ) and number of GSs ( g ), in the condition of astrocytic Na + -K + -ATPase inhibition. h – j Representative peri-event raster histograms and statistical data of firing rate of pyramidal neurons in GFAP-ChR2 M1 mice in response to blue light stimulation during in vivo single-unit recording, in the condition of Na + -K + -ATPase inhibition by ouabain ( h , i ) or shRNA-knockdown ( j , k ). l , m Rest membrane potential of astrocytes ( l ) and their changes ( m ) before and after blue light stimulation in GFAP-ChR2 M1 slices under normal and KA incubation conditions. n In vivo CSF potassium concentration before and after KA incubation in GFAP-ChR2 M1 mice under sham and blue light stimulation conditions. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; # p < 0.05, ## p < 0.01, ### p < 0.001. Data shown as mean ± s.e.m. The number of mice used is indicated in figures. For detailed statistical information, see Supplementary Data . Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-34662-2'>36414629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-13020_2022_639_fig7_html.jpgAnti-GFAP Antibody Picoband&reg;BSHX decoction decreased the damage of tissue and promoted axon regeneration after SCI. A Co-immunofluorescence images showed GFAP (red) and MAP2 (green) at day 14 after SCI. B Co-immunofluorescence images showed the axonal regeneration (GFAP, red; GAP43, green) in the lesion site at day after SCI. FS Fibrotic scar <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-022-00639-y'>35820953</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41467_2022_34662_fig6_html.pngAnti-GFAP Antibody Picoband&reg;Optogenetic-driven astrocytic Na + -K + -ATPase rescues seizure susceptibility of FCD rats. a Establishment of FCD model in SD rats and timeline design for behavioral experiments. b Representative immunostaining images showed NeuN + cells in nondysplastic (control) and dysplastic (FCD) cortex. Scale bar, 50 μm and 25 μm. c Representative cortical EEG traces recorded from dysplastic FCD rat showed several forms of spontaneous epileptic waves. d Representative energy spectra from control and FCD rats injected with PTZ. e – h Seizure susceptibility of control and FCD rats was tested after PTZ injection (i.p. injection, 60 mg/kg) and seizure stage ( e ), EEG onset ( f ), latency to GS ( g ) and GS duration ( h ) were recorded. i Fluorescent images showed the expression pattern of Na + -K + -ATPase protein (ATP1A2) in control and FCD cortical tissue. j Representative western blot bands and quantification of Na + -K + -ATPase protein (ATP1A2) expression in control and FCD cortex. GAPDH was used as the internal control. k FCD rats were injected with ChR2-EYFP and/or pAAV-shortGFAP-MCS-EGFP-3FLAG-mir30shRNA(Atp1α2) in the M1 and implanted with fiber and electrode for blue light stimulation and EEG recording. l Fluorescent images showed shRNA(Atp1α2) co-localization with GFAP + astrocytes and knockdown of astrocytic Na + -K + -ATPase protein (ATP1A2) expression in FCD cortical tissue. Scale bar, 20 μm. m – p Effects of optogenetic stimulation of astrocytes on seizure stage ( m ), EEG onset ( n ), latency to GS ( o ) and GS duration ( p ) in FCD-ChR2 M1 rats, in the condition of astrocytic Na + -K + -ATPase inhibition by shRNA-knockdown. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with each control group. Data shown as mean ± s.e.m. The number of mice used is indicated in figures. For detailed statistical information, see Supplementary Data . Uncropped version of western blots that labeled with the relevant panel and antibody were provided in Source data western blot. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-34662-2'>36414629</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-13020_2022_639_fig10_html.pngAnti-GFAP Antibody Picoband&reg;BSHX decoction promoted NSCs proliferation and neuronal differentiation. A , D BrdU labeling analysis in NSCs proliferation. B , E Immunofluorescent staining of GFAP after neural differentiation. C , F Immunofluorescent staining of NeuN after neural differentiation. All experiments were performed in triplicated and data were presented as means ± SD, n = 3 per group. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-022-00639-y'>35820953</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-fnagi-14-866336-g003.jpgAnti-GFAP Antibody Picoband&reg;Physical exercise decreased the number of A1 neurotoxic astrocytes. (A) Representative immunofluorescence images for GFAP (green) and C3 (red) double staining of rats in each group in the corpus callosum. Scale bar = 50 μm. (B) The quantification of GFAP positive astrocytes and (C) C3/GFAP positive A1 astrocytes. n = 6. Experimental data are expressed as the means ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. GFAP, glial fibrillary acidic protein; C3, complement 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9198634/&orig_host=www.ncbi.nlm.nih.gov'>35721009</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-fnagi-14-866336-g004.jpgAnti-GFAP Antibody Picoband&reg;Physical exercise enhanced the expression of A2 neuroprotective astrocytes. (A) Representative immunofluorescence images for GFAP (green) and S100A10 (red) double staining of rats in each group in the corpus callosum. Scale bar = 50 μm. (B) The quantification of GFAP positive astrocytes and (C) S100A10/GFAP positive A2 astrocytes. n = 6. Experimental data are expressed as the means ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. S100A10, S100 calcium binding protein A10.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9198634/&orig_host=www.ncbi.nlm.nih.gov'>35721009</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-fnagi-14-866336-g005.jpgAnti-GFAP Antibody Picoband&reg;Physical exercise augmented the expression and length of primary cilia in astrocytes. (A) Representative immunofluorescence images for GFAP (green) and ARL13B (red) double staining of rats in each group in the corpus callosum. Scale bar = 50 μm. (B) Quantification of the percentage of astrocytes containing primary cilia. (C) Quantification of length of astrocytic primary cilia. n = 6. Experimental data are expressed as the means ± SD. * P < 0.05. ARL13B, ADP-ribosylation factor-like 13B.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9198634/&orig_host=www.ncbi.nlm.nih.gov'>35721009</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12974_2021_2248_fig4_html.pngAnti-GFAP Antibody Picoband&reg;TUDCA decreased the damage of tissue and neurons after SCI. A Co-immunofluorescence images showed GFAP (red) and MAP2 (green) 14 days after SCI. B Quantification of the distance from neurons to the lesion center from MAP2 immunofluorescence. C Quantification of the fibrotic scar surrounding by reactive astrocytes of spinal cord from GFAP immunofluorescence. ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-021-02248-2'>34544428</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12974_2021_2248_fig5_html.pngAnti-GFAP Antibody Picoband&reg;TUDCA promoted axonal regeneration after SCI. A Co-immunofluorescence images showed the loss of neurons NeuN (red) and axon regeneration GAP43 (green) in the lesion site 14 days after SCI. B Co-immunofluorescence images showed the axonal regeneration (GAP43, green; GFAP, red) in the fibrotic scar on day 14 post-SCI. C – E Western blot analysis and quantification of GFAP and GAP43 expression. All experiments were performed in triplicated and data were presented as means ± SD, n =3 per group. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-021-02248-2'>34544428</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12974_2021_2248_fig6_html.pngAnti-GFAP Antibody Picoband&reg;TUDCA treatment promoted remyelination. A . Immunofluorescent images of spinal cord on day 14 post-SCI showing the distribution of MBP (green) and GFAP in the lesion site. B , C Western blot analysis and quantification data of MBP expression in each group. All experiments were performed in triplicated and data were presented as means ± SD, n =3 per group. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-021-02248-2'>34544428</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-ajtr0012-5752-f1.jpgAnti-GFAP Antibody Picoband&reg;In vitro differentiation of NSCs. Immunofluorescence staining of adherent neurospheres showed positive staining for Nestin (A). Immunofluorescence staining of differentiated NSCs showed positive staining for astrocyte marker GFAP (B) and neuron marker Tuj1 (C). Cell nuclei were counterstained with DAPI (blue). DAPI, 4’,6-diamidino-2-phenylindole.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7540113/&orig_host=www.ncbi.nlm.nih.gov'>33042454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-ajtr0012-5752-f2.jpgAnti-GFAP Antibody Picoband&reg;The increase of let-7f expression level is age-dependent. A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 in brains of rats at three different ages (Postnatal Day 1 (P1), P8 and P14), with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected by qRT-PCR in brains of P1, P8 and P14 rats. Data shown as mean ± SD. * P <0.05 vs. P1; # P <0.05 vs. P8. Data representative of three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7540113/&orig_host=www.ncbi.nlm.nih.gov'>33042454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-ajtr0012-5752-f3.jpgAnti-GFAP Antibody Picoband&reg;The increase of let-7f expression level is associated with differentiation of NSCs in vitro . A. Representative western blotting showing the protein expressions of Nestin, GFAP and Tuj1 following the induction of NSCs on days 0, 3, 5 and 7, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. B. Let-7f expression level detected in NSCs subject to differentiation on days 0, 3, 5 and 7 by qRT-PCR. Data shown as mean ± SD. * P <0.05 vs. 0 d; # P <0.05 vs. 3 d; Ψ P <0.05 vs. 5 d. Data representative of three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7540113/&orig_host=www.ncbi.nlm.nih.gov'>33042454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-ajtr0012-5752-f5.jpgAnti-GFAP Antibody Picoband&reg;Leverage of let-7 expression level has an impact on the differentiation of NSCs. A. Expression of EGFP was observed in transduced cells under fluorescence microscope. Uninfected cells were used as controls. B. Representative western blotting showing the expressions of Nestin, GFAP and Tuj1 in uninfected and infected differentiated NSCs, with densitometric analysis reflecting the expression levels of Nestin, GFAP and Tuj1. Data shown as mean ± SD. * P <0.05 vs. uninfected; # P <0.05 vs. vector; Ψ P <0.05 vs. let-7f mimic. Data representative of three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7540113/&orig_host=www.ncbi.nlm.nih.gov'>33042454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41419_2019_1434_fig2_html.pngAnti-GFAP Antibody Picoband&reg;TIGAR is specifically increased during differentiation of NSCs. a – c Quantification of the mRNA levels of Tigar , Nestin , and Sox2 during proliferation of NSCs. d – f Quantification of the mRNA levels of Tigar , Gfap and Map2 during differentiation of NSCs. * p < 0.05, ** p < 0.01, one-way ANOVA. Data represent the mean of at least three independent experiments ± SEM <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-019-1434-3'>30814486</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41419_2019_1434_fig3_html.pngAnti-GFAP Antibody Picoband&reg;TIGAR regulates the differentiation of NSCs. a Quantification of the mRNA levels of Tigar , Map2 , Gfap , Neurod1 , Ngn1 , and Rest in the Lenti- siSCR - and Lenti- siTigar -treated groups of cultured NSCs. b , c Representative immunoblots and relative quantification of Tuj1, GFAP, and TIGAR after knockdown of TIGAR in NSCs. d Quantification of the mRNA levels of Tigar , Map2 , Gfap , Neurod1 , Ngn1 , and Rest in the Lenti- Gfp - and Lenti- Tigar -treated groups in cultured NSCs. e , f Representative immunoblots and relative quantification of Tuj1, GFAP, and TIGAR after overexpression of TIGAR in NSCs. g Double immunofluorescent staining showed that knockdown of TIGAR (GFP, green) decreased the expression of Tuj1 (red) and impaired neuronal differentiation of NSCs. The right panel shows the quantitative ratio of Tuj1 + GFP + to GFP + cells in the Lenti- siSCR and Lenti- siTigar groups. h Double immunofluorescent staining revealed that knockdown of TIGAR (GFP, green) also decreased expression of GFAP (red) and inhibited astrocytes differentiation of NSCs. The right panel shows the quantitative ratio of GFAP + GFP + to GFP + cells in the Lenti- siSCR and Lenti- siTigar groups. Scale bar = 50 μm. * p < 0.05, ** p < 0.01, t -test. Data represent the mean of at least three independent experiments ± SEM <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-019-1434-3'>30814486</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41419_2019_1434_fig4_html.pngAnti-GFAP Antibody Picoband&reg;TIGAR has no effect on the cell survival or proliferation of NSCs. a , b Quantification of the mRNA levels of Sox2 , Nestin , Ki67 , Map2 , and Gfap in the Lenti- Gfp - and Lenti- Tigar -treated groups of proliferated NSCs. c TUNEL staining (green) in cultured NSCs treated with Lenti- siSCR or Lenti- siTigar during the differentiation stage. The right panel shows the quantification of TUNEL-positive cells in the Lenti- siSCR and Lenti- siTigar groups. Scale bar = 50 μm. d During the differentiation stage, there was no significant difference in the number of dividing NSCs in the Lenti- siSCR and Lenti- siTigar groups. The right panel shows the quantification of EdU-positive cells in the Lenti- siSCR and Lenti- siTigar groups. Scale bar = 50 μm. * p < 0.05, ** p < 0.01, t -test. Data represent the mean of at least three independent experiments ± SEM <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-019-1434-3'>30814486</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41419_2019_1434_fig6_html.pngAnti-GFAP Antibody Picoband&reg;TIGAR promotes NSC differentiation by upregulating the level of acetyl-CoA and the acetylation of H3K9. a Western blot analysis of whole-cell lysates showed that H3K9 acetylation was reduced after treatment with Lenti- siTigar . b Western blot analysis showed that Lenti- Tigar significantly increased H3K9 acetylation during NSC differentiation. c Lenti- siTigar decreased the accumulation of H3K9ac on the promoters of Ngn1 , Neurod1 , and Gfap . NSCs were immunoprecipitated with anti-acetyl-H3K9 antibody and analyzed using gene-specific ChIP primers. Rabbit IgG was used as a negative control. DNA from each ChIP sample was normalized to the corresponding input sample. d Acetyl-CoA production in Lenti- siSCR , Lenti- siSCR + Acetate, and Lenti- siTigar + Acetate group. e Western blot analysis showed that supplementation of acetate in cultured medium increased H3K9 acetylation and rescued the effect of Lenti- siTigar on the decrease in H3K9 acetylation in NSCs. f Quantification of the mRNA levels of Map2 and Gfap after treatment with acetate and Lenti- siTigar in cultured NSCs. Acetyl-CoA can be generated from acetate by acetyl-CoA synthetase independently of citrate. g Western blot analysis showed that acetate rescued the effect of Lenti- siTigar on the decrease in Tuj1 and GFAP protein levels. * p < 0.05, ** p < 0.01. Data represent the mean of at least three independent experiments ± SEM <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-019-1434-3'>30814486</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12974_2018_1260_fig5_html.pngAnti-GFAP Antibody Picoband&reg;Adam10 overexpression suppresses hippocampal neuroinflammation in TLE mice. a Western blotting showing the protein levels of inflammation-related proteins iNOS and COX-2 and NF-κB in the hippocampus of Vehicle Ctrl, AAV-Ctrl, and AAV-Adam10-treated TLE mice. b – d Bar graphs showing the quantification of iNOS ( F 2,12 = 9.86, p = 0.024, AAV-Adam10 vs Vehicle Ctrl; p = 0.003, AAV-Adam10 vs AAV-Ctrl), COX-2 ( F 2,12 = 11.27, p = 0.003, AAV-Adam10 vs Vehicle Ctrl; p = 0.007, AAV-Adam10 vs AAV-Ctrl), and NF-κB ( F 2,12 = 11.05, p = 0.004, AAV-Adam10 vs Vehicle Ctrl; p = 0.005, AAV-Adam10 vs AAV-Ctrl), which were represented as the intensity ratios of these proteins to β-actin ( n = 5). e , f Bar graphs showing the concentration of IL-1β ( F 2,12 = 0.59, p = 0.572) and TNF-α ( F 2,12 = 10.11, p = 0.004, AAV-Adam10 vs Vehicle Ctrl; p = 0.009, AAV-Adam10 vs AAV-Ctrl) in the hippocampus of Ctrl, AAV-Ctrl, and AAV-Adam10-treated TLE mice as detected by ELISA ( n = 5). g Representative images of the immunostaining of Iba-1 and GFAP in the hippocampal CA1 region of the Ctrl, AAV-Ctrl, and AAV-Adam10 mice, respectively. h , i Bar graphs showing the quantification of Iba-1- ( F 2,12 = 7.31, p = 0.012, AAV-Adam10 vs Vehicle Ctrl; p = 0.024, AAV-Adam10 vs AAV-Ctrl) and GFAP ( F 2,12 = 4.61, p = 0.039, AAV-Adam10 vs Vehicle Ctrl; p = 0.042, AAV-Adam10 vs AAV-Ctrl)-positive cells in the hippocampal CA1 region of the Ctrl, AAV-Ctrl, and AAV-Adam10 mice, respectively ( n = 4). * p < 0.05, ** p < 0.01, and one-way ANOVA. Scale bar = 50 μm in g <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-018-1260-z'>30075790</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41598_2015_article_bfsrep12943_fig4_html.jpgAnti-GFAP Antibody Picoband&reg;Chronic noise exposure causes persistent overexpression of GFAP in the hippocampus. ( A ) Comparison of GFAP mRNA expression levels at different time points in control and noise-exposed rats by quantitative real-time PCR. ( B,D ) Western blot analysis of GFAP and IBA1 under C (control) and N (chronic noise exposure) conditions at different time points. GAPDH was used as a loading control. The immunoreactive band density was quantified and presented as the percent change relative to control samples ( C,E ). Bars represent means ± S.D. *p < 0.05 and **p < 0.01, compared with respective controls by Student’s t -test (n = 6 per group). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep12943'>26251361</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-12974_2018_1260_fig8_html.pngAnti-GFAP Antibody Picoband&reg;Adam10 knockdown exacerbates hippocampal neuroinflammation in TLE mice. a Western blotting showing the protein levels of the inflammation-related proteins iNOS and COX-2 and NF-κB in the hippocampus of Vehicle Ctrl, shRNA-Ctrl, and shRNA-Adam10-treated TLE mice. b – d Bar graphs showing the quantification of iNOS ( F 2,12 = 32.09, p < 0.001, shRNA-Adam10 vs Vehicle Ctrl; p < 0.001, shRNA-Adam10 vs shRNA-Ctrl), COX-2 ( F 2,12 = 5.32, p = 0.035, shRNA-Adam10 vs Vehicle Ctrl; p = 0.041, shRNA-Adam10 vs shRNA-Ctrl), and NF-κB ( F 2,12 = 5.82, p = 0.020, shRNA-Adam10 vs Vehicle Ctrl; p = 0.049, shRNA-Adam10 vs shRNA-Ctrl), which were represented as the intensity ratios of these proteins to β-actin ( n = 5). e , f Bar graphs showing the concentration of IL-1β ( F 2,12 = 12.78, p = 0.003, shRNA-Adam10 vs Vehicle Ctrl; p = 0.003, shRNA-Adam10 vs shRNA-Ctrl) and TNF-α ( F 2,12 = 5.66, p = 0.035, shRNA-Adam10 vs Vehicle Ctrl; p = 0.030, shRNA-Adam10 vs shRNA-Ctrl) in the hippocampus of Vehicle Ctrl, shRNA-Ctrl, and shRNA-Adam10-treated TLE mice, as detected by ELISA ( n = 5). g Representative images of the immunostaining of Iba-1 and GFAP in the hippocampal CA1 region of the Vehicle Ctrl, shRNA-Ctrl, and shRNA-Adam10 mice, respectively. h , i Bar graphs showing the quantification of Iba-1- ( F 2,12 = 4.70, p = 0.047, shRNA-Adam10 vs shRNA-Ctrl) and GFAP ( F 2,12 = 4.88, p = 0.039, shRNA-Adam10 vs shRNA-Ctrl)-positive cells in the hippocampal CA1 region of the Vehicle Ctrl, shRNA-Ctrl, and shRNA-Adam10 mice, respectively ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001, and one-way ANOVA. Scale bar = 50 μm in g <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-018-1260-z'>30075790</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-41398_2018_176_fig4_html.jpgAnti-GFAP Antibody Picoband&reg;Neuron and astrocyte immunohistochemical analysis and mRNA expression of GABA A receptor subunits. a Outline of sagittal section of mouse brain, showing the brain regions analyzed using immunohistochemical staining. b – i Coronal sections stained through fluorescence-labeling of: b NeuN in anterior cingulate cortex (ACC); c NeuN in hippocampus (HC); d DCX in dentate gyrus (DG); e parvalbumin (PV) in ACC; f PV in piriform cortex (PC); g PV in DG; h GFAP in CA1 region; and i GFAP in DG. The DG, CA1, and CA3 regions in hippocampus are indicated in part ( c ). NeuN and PV immunofluorescence are displayed in green (scale bar = 300 μm), and that of DCX and GFAP and DCX displayed in red (scale bar = 120 μm). The plots show immunofluorescence densities for WT (green dots) and KO (red dots) estimated in terms of average area optical density (in parts b , c , e , h , and i ), or in terms of the number of immunoreactive cells (in parts d , f , g ); in each instance, five randomly selected images from each of five WT or KO male mice were examined. The levels of mRNA expression for 13 different GABA A receptor subunits in WT and KO mouse cerebrum ( j ) and cerebellum ( k ) were measured using quantitative RT-PCR, and the mRNA levels in KO mice were normalized to the average expression level in WT ( n = 10 in each group). The measured expression levels in WT, KO as well as HT brains are given in Supplementary Table . Statistical analysis was performed using unpaired t -test for immunohistochemical staining and one-way ANOVA with Newman–Keuls post-hoc test for mRNA quantitation. Average y values ± SEM in the different plots are represented by horizontal bars; WT is represented by green dots and KO by red dots. N.D. represents non-detectable; * p < 0.05, ** p < 0.01, *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41398-018-0176-9'>30013074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1239-2-IHC-anti-gfap-antibody.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PA1239).<br> GFAP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-gfap-primary-antibodies-ihc-testing-3_1.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PA1239).<br> GFAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-gfap-primary-antibodies-ihc-testing-4.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PA1239).<br> GFAP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-3_5.jpgAnti-GFAP Antibody Picoband&reg; IF analysis of GFAP using anti-GFAP antibody (PA1239).<br> GFAP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-gfap-primary-antibodies-ihc-testing-6.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PA1239). <br> GFAP was detected in a spinal cord. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% horse serum. The tissue section was then incubated with anti-GFAP antibody (PA1239) at 1:500 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using confocal microscope.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-gfap-primary-antibodies-ihc-testing-7.pngAnti-GFAP Antibody Picoband&reg;IF analysis of GFAP using anti-GFAP antibody (PA1239). <br> GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. DyLight 550-conjugated Goat Anti-Rabbit was used as secondary antibody incubated for 1 hour at RT. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-8-antibody-pa1240-boster.html2026-03-24T05:03:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1240-krt8-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 8/KRT8 Antibody Picoband&reg; Western blot analysis of KRT8 using anti-KRT8 antibody (PA1240). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human CACO-2 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: huamn Jurkat whole cell lysates,<br> Lane 5: rat RH35 whole cell lysates,<br> Lane 6: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT8 antigen affinity purified polyclonal antibody (Catalog # PA1240) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT8 at approximately 54 kDa. The expected band size for KRT8 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1240-krt8-primary-antibodies-ihc-testing-2.jpgAnti-Cytokeratin 8/KRT8 Antibody Picoband&reg; IHC analysis of ITGB3 using anti-ITGB3 antibody (PA1240). <br> ITGB3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGB3 Antibody (PA1240) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1240-krt8-primary-antibodies-fcm-testing-3.jpgAnti-Cytokeratin 8/KRT8 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-KRT8 antibody (PA1240). <br> Overlay histogram showing CACO-2 cells stained with PA1240 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KRT8 Antibody (PA1240, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-receptor-ii-antibody-pa1243-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1243-1-WB-anti-stnfsr-ii-antibody.jpgAnti-TNF Receptor II/TNFRSF1B Antibody Picoband&reg;Anti-TNF Receptor II antibody&#44; PA1243&#44; Western blotting<br>Lane 1: MM453 Cell Lysate<br>Lane 2: JURKAT Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1243-2-IHC-anti-stnfsr-ii-antibody.jpgAnti-TNF Receptor II/TNFRSF1B Antibody Picoband&reg;Anti-TNF Receptor II antibody&#44; PA1243&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-d1-antibody-pa1245-1-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1245-1-1-WB-anti-cyclin-d1-antibody.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;Anti-Cyclin D1 antibody&#44; PA1245-1&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: Rat Ovary Tissue Lysate<br>Lane 3: Rat Brain Tissue Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: MM231 Cell Lysate<br>Lane 6: SW620 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1245-1-medscimonit-24-2384-g002.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;Up-regulation of LncRNA SRA promoted cell proliferation. ( A ) Cell proliferation ability was determined by CCK-8 assay. ( B ) Cell cycle was assessed by flow cytometry. ( C ) Western blot analysis of Cyclin B, Cyclin E, and Cyclin D1 expression. Data shown as mean ±SD. ** P <0.01; *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5928913/'>29674607</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lxr-alpha-antibody-pa1246-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1246-1-WB-anti-lxr-alpha-antibody.jpgAnti-LXR alpha/NR1H3 Antibody Picoband&reg;Anti-LXR alpha antibody&#44; PA1246&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: MCF-7 Cell Lysate <br>Lane 3: HELA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lxr-alpha-antibody-pa1246-1-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1246-1-1-WB-anti-lxr-alpha-antibody.jpgAnti-LXR alpha/NR1H3 Antibody Picoband&reg;Anti-NR1H3 antibody&#44; PA1246-1&#44; Western blotting<br>All lanes: Anti NR1H3 (PA1246-1) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: Rat Lung Tissue Lysate at 50ug<br>Lane 3: Rat Spleen Tissue Lysate at 50ug<br>Lane 4: Rat Kidney Tissue Lysate at 50ug<br>Lane 5: Human Placenta Tissue Lysate at 50ug<br>Predicted bind size: 51KD<br>Observed bind size: 51KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tfpi2-antibody-pa1248-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1248-tfpi2-primary-antibodies-wb-testing-1.jpgAnti-TFPI2 Antibody Picoband&reg;Western blot analysis of TFPI2 using anti-TFPI2 antibody (PA1248). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFPI2 antigen affinity purified polyclonal antibody (PA1248) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFPI2 at approximately 27-30 kDa. The expected band size for TFPI2 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1248-tfpi2-primary-antibodies-ihc-testing-1.jpgAnti-TFPI2 Antibody Picoband&reg;IHC analysis of TFPI2 using anti-TFPI2 antibody (PA1248). <br>TFPI2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TFPI2 Antibody (PA1248) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-3-p10-antibody-pa1302-1-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1302-1-1-IHC-anti-caspase-3-p10-antibody.jpgAnti-Caspase-3 (P10)/CASP3 Antibody Picoband&reg;Anti-Caspase-3(P10)&#44; PA1302-1&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1302-1-2-WB-anti-caspase-3-p10-antibody.jpgAnti-Caspase-3 (P10)/CASP3 Antibody Picoband&reg;Anti-Caspase-3(P10)&#44; PA1302-1&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: SMMC Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1302-1-3-IHC-anti-caspase-3-p10-antibody.jpgAnti-Caspase-3 (P10)/CASP3 Antibody Picoband&reg;Anti-Caspase-3(P10)&#44; PA1302-1&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1302-1-fphar-10-01096-g006.jpgAnti-Caspase-3 (P10)/CASP3 Antibody Picoband&reg;Effects of ZXC on the mRNA levels of the Bcl-2/Bax ratio (A) , caspase-3 (B) , nuclear factor (NF)-кB (C) , and p38 (D) in the prefrontal cortex of ischemia-reperfusion injury rats. The data are expressed as mean ± standard deviation (n = 3). I-30, ischemia for 30 min; I-90, ischemia for 90 min; I-90+R-30, ischemia for 90 min, then reperfusion for 30 min; I-90+R-180, ischemia for 90 min, then reperfusion for 180 min. * P < 0.05 vs. sham group; # P < 0.05 vs. model group; ## P < 0.01 vs. model group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.01096/full'>31611791</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1302-1-fphar-10-01096-g007.jpgAnti-Caspase-3 (P10)/CASP3 Antibody Picoband&reg;Western blotting results for Sham, Model and ZXC groups (A) . Effects of ZXC on the protein expressions Bcl-2 (B) , Bax (C) , Caspase-3 (D) , NF-кB (E) , and p38 (F) in brain tissue of ischemia-reperfusion injury rats induced by MCAO. The data are expressed as mean ± standard deviation (n = 4). I-30, ischemia for 30 min; I-90, ischemia for 90 min; I-90+R-30, ischemia for 90 min, then reperfusion for 30 min. *P < 0.05 vs. sham group; # P < 0.05 vs. model group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.01096/full'>31611791</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1302-1-4.jpgAnti-Caspase-3 (P10)/CASP3 Antibody Picoband&reg; ICC analysis of Caspase-3(P10) using anti-Caspase-3(P10) antibody (PA1302-1). <br> Caspase-3(P10) was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Caspase-3(P10) Antibody (PA1302-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1302-1-p57_cdkn1c-primary-antibodies-ihc-testing-4.jpgAnti-Caspase-3 (P10)/CASP3 Antibody Picoband&reg; IHC analysis of caspase-3 (P10)/CASP3 using anti-caspase-3 (P10)/CASP3 antibody (PA1302-1). <br> caspase-3 (P10)/CASP3 was detected in a rat spinal cord. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with peroxidase blocker. The tissue section was then incubated with 2 μg/ml anti-caspase-3 (P10)/CASP3 antibody (PA1302-1) at 1:200 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated at 1:500 for 1h at room temperature. The tissue section was developed using fluorescent microscope. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s100-beta-antibody-pa1303-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1303-1-WB-anti-s100-beta-antibody.jpgAnti-S100 beta/S100B Antibody Picoband&reg;Anti-S100 beta antibody&#44; PA1303&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: MCF-7 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: SMMC Cell Lysate<br>Lane 6: JURKAT Cell Lysate<br>Lane 7: COLO320 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnfaip1-antibody-pa1305-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1305-tnfaip1-primary-antibodies-wb-testing-1.jpgAnti-TNFAIP1 Antibody Picoband&reg; Western blot analysis of TNFAIP1 using anti-TNFAIP1 antibody (PA1305). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: mouse HEPA1-6 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFAIP1 antigen affinity purified polyclonal antibody (Catalog # PA1305) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFAIP1 at approximately 36 kDa. The expected band size for TNFAIP1 is at 25,36 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1305-2-IHC-anti-tnfaip1-antibody.jpgAnti-TNFAIP1 Antibody Picoband&reg; IHC analysis of TNFAIP1 using anti-TNFAIP1 antibody (PA1305). <br> TNFAIP1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFAIP1 Antibody (PA1305) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1305-3-IF-anti-tnfaip1-antibody.jpgAnti-TNFAIP1 Antibody Picoband&reg; IF analysis of TNFAIP1 using anti-TNFAIP1 antibody (PA1305). <br> TNFAIP1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-TNFAIP1 Antibody (PA1305) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1305-4.jpgAnti-TNFAIP1 Antibody Picoband&reg; IHC analysis of TNFaIP1 using anti-TNFaIP1 antibody (PA1305). <br> TNFaIP1 was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFaIP1 Antibody (PA1305) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1305-5.jpgAnti-TNFAIP1 Antibody Picoband&reg; IHC analysis of TNFaIP1 using anti-TNFaIP1 antibody (PA1305). <br> TNFaIP1 was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFaIP1 Antibody (PA1305) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1305-6.jpgAnti-TNFAIP1 Antibody Picoband&reg; IHC analysis of TNFaIP1 using anti-TNFaIP1 antibody (PA1305). <br> TNFaIP1 was detected in paraffin-embedded section of human pancreas cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TNFaIP1 Antibody (PA1305) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-8-p18-antibody-pa1308-boster.html2026-03-24T05:03:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1308-1-WB-anti-caspase-8-p18-antibody.jpgAnti-Caspase-8 (P18)/CASP8 Antibody Picoband&reg;Anti-Caspase-8(P18) antibody&#44; PA1308&#44; Western blotting<br>All lanes: Anti Caspase-8(P18) (PA1308) at 0.5ug/ml<br>Lane 1: Rat Thymus Tissue Lysate at 50ug<br>Lane 2: Rat Liver Tissue Lysate at 50ug<br>Lane 3: MCF-7 Whole Cell Lysate at 40ug<br>Lane 4: HELA Whole Cell Lysate at 40ug<br>Lane 5: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 55KD<br>Observed bind size: 55KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pln-antibody-pa1309-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1309-pln-primary-antibodies-wb-testing-1.jpgAnti-Phospholamban/PLN Antibody Picoband&reg; Western blot analysis of PLN using anti-PLN antibody (PA1309). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates, <br> Lane 2: rat heart tissue lysates, <br> Lane 3: mouse heart tissue lysates, <br> Lane 4: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLN antigen affinity purified polyclonal antibody (Catalog # PA1309) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected respectively for PLN at approximately 24 kDa and 12 kDa. The expected band size for PLN is at 6 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erk2-antibody-pa1310-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1310-erk2-primary-antibodies-wb-testing-1.jpgAnti-ERK2/MAPK1 Antibody Picoband&reg;Western blot analysis of ERK2/MAPK1 using anti-ERK2/MAPK1 antibody (PA1310). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human U2OS whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERK2/MAPK1 antigen affinity purified polyclonal antibody (PA1310) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERK2/MAPK1 at approximately 41 kDa. The expected band size for ERK2/MAPK1 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erk2-antibody-pa1310-1-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1310-1-1-WB-anti-erk2-antibody.jpgAnti-ERK2/MAPK1 Antibody Picoband&reg;Anti-ERK2 antibody&#44; PA1310-1&#44; All Western blotting<br>All lanes: Anti-MAPK1(PA1310-1) at 0.5ug/ml<br> Lane 1: Human Placenta Tissue Lysate at 40ug<br> Lane 2: Rat Liver Tissue Lysate at 40ug<br> Lane 3: Rat Brain Tissue Lysate at 40ug<br> Lane 4: Rat Thymus Tissue Lysate at 40ug<br> Lane 5: HELA Whole Cell Lysate at 40ug<br> Lane 6: SMMC Whole Cell Lysate at 40ug<br> Lane 7: COLO320 Whole Cell Lysate at 40ug<br> Lane 8: MM231 Whole Cell Lysate at 40ug<br> Lane 9: CEM Whole Cell Lysate at 40ug<br> Predicted bind size: 41KD<br> Observed bind size: 41KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-puma-antibody-pa1313-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1313-1-WB-anti-puma-antibody.jpgAnti-PUMA/BBC3 Antibody Picoband&reg;Anti-PUMA antibody&#44; PA1313&#44; Western blotting<br>All lanes: Anti PUMA(PA1313) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: Rat Kidney Tissue Lysate at 50ug<br>Predicted bind size: 21KD<br>Observed bind size: 21KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-decorin-antibody-pa1314-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1314-1_1.jpgAnti-Decorin/DCN Antibody Picoband&reg; Western blot analysis of Decorin using anti-Decorin antibody (PA1314). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human SW620 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat PC-12 whole cell lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse SP2/0 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody (Catalog # PA1314) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for Decorin at approximately 40-50KD and 80-140KD. The expected band sizes for Decorin are at 40-50KD and 80-140KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-snap25-antibody-pa1315-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1315-1-WB-anti-snap25-antibody.jpgAnti-SNAP25 Antibody Picoband&reg;Anti-SNAP25 antibody&#44; PA1315&#44; Western blotting<br>WB: Rat Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1315-2-IHC-anti-snap25-antibody.jpgAnti-SNAP25 Antibody Picoband&reg;Anti-SNAP25 antibody&#44; PA1315&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mtco1-antibody-pa1317-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1317-mtco1-primary-antibodies-wb-testing-1.jpgAnti-MTCO1/MT-CO1 Antibody Picoband&reg; Western blot analysis of MTCO1 using anti-MTCO1 antibody (PA1317). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human COLO320 whole cell lysates, <br> Lane 3: human SMMC whole cell lysates, <br> Lane 4: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCO1 antigen affinity purified polyclonal antibody (Catalog # PA1317) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37 kDa. The expected band size for MTCO1 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mtco1-antibody-pa1317-1-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1317-1-1_1.jpgAnti-MTCO1/MT-CO1 Antibody Picoband&reg; Western blot analysis of MTCO1 using anti-MTCO1 antibody (PA1317-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The samples were loaded under reducing conditions. <br> Lane 1: human HeLa mitochondria lysates at 20ug&#44; <br> Lane 2: human HeLa whole cell lysates at 20ug&#44; <br> Lane 3: human Caco-2 whole cell lysates at 50ug&#44;<br> Lane 4: rat heart tissue lysates at 50ug. <br> Lane 5: mouse heart tissue lysates at 50ug. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTCO1 antigen affinity purified polyclonal antibody (Catalog # PA1317-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTCO1 at approximately 37KD. The expected band size for MTCO1 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1317-1-2-IHC-anti-mtco1-antibody.jpgAnti-MTCO1/MT-CO1 Antibody Picoband&reg; IHC analysis of MTCO1 using anti-MTCO1 antibody (PA1317-1).<br> MTCO1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTCO1 Antibody (PA1317-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1317-1-3-IF-anti-mtco1-antibody.jpgAnti-MTCO1/MT-CO1 Antibody Picoband&reg; IHC analysis of MTCO1 using anti-MTCO1 antibody (PA1317-1).<br> MTCO1 was detected in immunocytochemical section of C6 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-MTCO1 Antibody (PA1317-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mtco1-antibody-pa1317-2-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1317-2-1-WB-anti-mtco1-antibody.jpgAnti-MTCO1/mt-Co1 Antibody Picoband&reg;Anti-MTCO1 antibody&#44; PA1317-2&#44; Western blotting<br>Lane 1: Rat Heart Tissue Lysate<br>Lane 2: Mouse Heart Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1317-2-2-IHC-anti-mtco1-antibody.jpgAnti-MTCO1/mt-Co1 Antibody Picoband&reg;Anti-MTCO1 antibody&#44; PA1317-2&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1317-2-3-IHC-anti-mtco1-antibody.jpgAnti-MTCO1/mt-Co1 Antibody Picoband&reg;Anti-MTCO1 antibody&#44; PA1317-2&#44; IHC(P)<br>IHC(P): Mouse Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1317-2-4-IHC-anti-mtco1-antibody.jpgAnti-MTCO1/mt-Co1 Antibody Picoband&reg;Anti-MTCO1 antibody&#44; PA1317-2&#44; IHC(P)<br>IHC(P): Mouse Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1317-2-5-IF-anti-mtco1-antibody.jpgAnti-MTCO1/mt-Co1 Antibody Picoband&reg;Anti-MTCO1 antibody&#44; PA1317-2&#44; ICC<br>ICC: HEPA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1317-2-mt-co1-primary-antibodies-ihc-testing-4.jpgAnti-MTCO1/mt-Co1 Antibody Picoband&reg;IHC analysis of MT-CO1 using anti-MT-CO1 antibody (PA1317-2). <br>MT-CO1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MT-CO1 Antibody (PA1317-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-fos-antibody-pa1318-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1318-1-WB-anti-c-fos-antibody.jpgAnti-c-Fos Antibody Picoband&reg;Anti-c-Fos antibody&#44; PA1318&#44; Western blotting<br>Lane 1: HT1080 Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1318-fcell-12-1503481-g002.jpgAnti-c-Fos Antibody Picoband&reg;DFSC-EVs regulated tooth eruption by inhibiting osteoclast differentiation. (A) Schematic illustration of RAW264.7 and DFSC co-culture system. (B) Representative images of TRAP staining. Scale bar = 200 μm. (C) Quantitative analysis of TRAP-positive area. (D) The mRNA level of ACP5 , CTSK and CFOS in RAW264.7 cultured with DFSC. (E) The protein level of ACP5, CTSK and CFOS in RAW264.7 cultured with DFSC. (F) Western blotting quantification. (G) Schematic illustration of RAW264.7 and DFSC-EVs co-culture system. (H) Representative images of TRAP staining. Scale bar = 200 μm. (I) Quantitative analysis of TRAP-positive area. (J) The mRNA level of ACP5 , CTSK and CFOS in RAW264.7 cultured with DFSC-EVs. (K) The protein level of ACP5, CTSK and CFOS in RAW264.7 cultured with DFSC-EVs. (L) Western blotting quantification. ns, not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1318-fcell-12-1503481-g003.jpgAnti-c-Fos Antibody Picoband&reg;ANXA1 was the core factor of DFSC-EVs regulating osteoclast differentiation. (A) Gene ontology enrichment analysis of DFSC-EVs protein profiles. (B) The top proteins of Cadherin related to regulating osteoblast differentiation based on expression level. (C) The mRNA level of ANXA1 . (D) The protein level of ANXA1. (E) Western blotting quantification. (F) Schematic illustration of RAW264.7 and siANXA1-EVs co-culture system. (G) Representative images of TRAP staining. Scale bar = 200 μm. (H) Quantitative analysis of TRAP-positive area. (I) The mRNA level of ACP5 , CTSK and CFOS in RAW264.7 cultured with siANXA1-EVs. (J) The protein level of ACP5, CTSK and CFOS in RAW264.7 cultured with siANXA1-EVs. (K) Western blotting quantification. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1318-fcell-12-1503481-g004.jpgAnti-c-Fos Antibody Picoband&reg;ANXA1 mediated PPARγ-CEBPα pathway to regulate osteoclast differentiation (A) The mRNA level of PPARγ in RAW264.7 cultured with siANXA1-EVs. (B) The mRNA level of CEBPα in RAW264.7 cultured with siANXA1-EVs. (C) The protein level of PPARγ and CEBPα in RAW264.7 cultured with siANXA1-EVs. (D) Quantitative analysis of PPARγ protein expression. (E) Quantitative analysis of CEBPα protein expression. (F) Schematic illustration of PPARγ inhibited RAW264.7 and DFSC-EVs co-culture system. (G) Representative images of TRAP staining. Scale bar = 200 μm. (H) Quantitative analysis of TRAP-positive area. (I) PPARγ inhibited RAW264.7 construction. (J) The mRNA level of CEBPα in PPARγ inhibited RAW264.7. (K) The protein level of PPARγ and CEBPα in PPARγ inhibited RAW264.7. (L) Quantitative analysis of PPARγ protein expression. (M) Quantitative analysis of CEBPα protein expression. (N) The mRNA level of ACP5 , CTSK and CFOS in PPARγ inhibited RAW264.7. (O) The protein level of ACP5, CTSK and CFOS in PPARγ inhibited RAW264.7. (P) Western blotting quantification. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1318-fphar-14-1181133-g006.jpgAnti-c-Fos Antibody Picoband&reg;The pretreatment of RD-6 inhibited the IL-17 signaling pathway in indomethacin-induced GU rats. The expression of IL17RA, FOS, IL1B, and PTGS2 determined in gastric tissue by qRT-PCR (A) and western bloting (B) . Data are expressed as mean ± S.E.M ( n = 3). One-way ANOVA with the uncorrected Fisher’s LSD test was used to evaluate multiple comparisons. # p < 0.05, ## p < 0.01 vs. NC group; * p < 0.05, ** p < 0.01 vs. IND group. NC, normal control; IND, indomethacin; RD-6-L, M, and H represent Ruda-6 at low, medium and high doses, respectively.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10449537/&orig_host=www.ncbi.nlm.nih.gov'>37637418</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1318-13020_2022_627_fig10_html.pngAnti-c-Fos Antibody Picoband&reg;Effects of JTG on expression of associated proteins and NF-κB pathway of osteoclast induced from BMMs with RANKL and LPS. BMMs were incubated with RANKL and JTG for 48 h, the proteins were extracted to analyze associated proteins of osteoclast by Western blot. A : a Western blot imagines for expression of NFATc1, c-Fos, Cathepsin K and MMP9. A : b – e The quantification analysis of NFATc1, c-Fos, Cathepsin K and MMP9 based on the results of A : a by ECL detection system, respectively. B : a The images of Western blot for TRAF6, P-P65, P65 and IκBα. B : b – d The quantification analysis of TRAF6, P-P65/P65 and IκBα based on the results of B : a by using an ECL detection system, respectively. Each point represents the mean ± SD (n = 3). The experiments were repeated for three times. * P < 0.05, ** P < 0.01 compared with control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-022-00627-2'>36195960</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppar-gamma-antibody-pa1320-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1320-1-WB-anti-ppar-gamma-antibody.jpgAnti-PPAR gamma/PPARG Antibody Picoband&reg;Anti-PPAR gamma antibody&#44; PA1320&#44; Western blotting<br>Lane 1: MM453 Cell Lysate<br>Lane 2: MM231 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br>Lane 5: HT1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nphs2-antibody-pa1322-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1322-1-WB-anti-nphs2-podocin-antibody.jpgAnti-Podocin NPHS2 Antibody Picoband&reg;Anti-NPHS2 antibody&#44; PA1322&#44; Western blotting<br>WB: Rat Kidney Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nphs2-antibody-pa1322-1-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1322-1-1-WB-anti-nphs2-podocin-antibody.jpgAnti-Podocin NPHS2 Antibody Picoband&reg; Western blot analysis of NPHS2 using anti-NPHS2 antibody (PA1322-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPHS2 antigen affinity purified polyclonal antibody (Catalog # PA1322-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPHS2 at approximately 45KD. The expected band size for NPHS2 is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1322-1-2-IHC-anti-nphs2-podocin-antibody.jpgAnti-Podocin NPHS2 Antibody Picoband&reg; IHC analysis of NPHS2 using anti-NPHS2 antibody (PA1322-1).<br> NPHS2 was detected in frozen section of rat kidney tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NPHS2 Antibody (PA1322-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1322-1-nphs2-primary-antibodies-ihc-testing-4.jpgAnti-Podocin NPHS2 Antibody Picoband&reg;IHC analysis of NPHS2 using anti-NPHS2 antibody (PA1322-1). <br>NPHS2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPHS2 Antibody (PA1322-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1322-1-3.jpgAnti-Podocin NPHS2 Antibody Picoband&reg; Western blot analysis of NPHS2 using anti-NPHS2 antibody (PA1322-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 70 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPHS2 antigen affinity purified polyclonal antibody (Catalog # PA1322-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPHS2 at approximately 45KD. The expected band size for NPHS2 is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1322-1-nphs2-primary-antibodies-ihc-testing-5.jpgAnti-Podocin NPHS2 Antibody Picoband&reg;IHC analysis of NPHS2 using anti-NPHS2 antibody (PA1322-1). <br>NPHS2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NPHS2 Antibody (PA1322-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1322-1-4.jpgAnti-Podocin NPHS2 Antibody Picoband&reg; IHC analysis of NPHS2 using anti-NPHS2 antibody (PA1322-1). <br> NPHS2 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NPHS2 Antibody (PA1322-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1322-1-5.jpgAnti-Podocin NPHS2 Antibody Picoband&reg; IHC analysis of NPHS2 using anti-NPHS2 antibody (PA1322-1). <br> NPHS2 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NPHS2 Antibody (PA1322-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1322-1-13659_2024_446_fig4_html.pngAnti-Podocin NPHS2 Antibody Picoband&reg;Scutellarin Restored Podocyte Injury of the DN Mice. a Representative images of immunohistochemistry for NPHS1 and NPHS2 of the mice treated with vehicle, scutellarin or empagliflozin (× 200; scale bar = 50 µm). b Representative images of Western-blotting for NPHS1, NPHS2. c Quantitative plot of the expression of NPHS1 of the mice. d Quantitatification of NPHS1 expression of the mice. e Representative images of Western-blotting for β-catenin, Axin2, snail and DKK1 of the mice. f – i Quantifications of the protein levels for β-catenin, Axin2, snail and DKK1 from E. All data are presented as the mean ± S.D.; n = 4–6 for each group, “n” stands for the number of animals; p vs. the model group (STZ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s13659-024-00446-y'>38656633</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-muscarinic-acetylcholine-receptor-2-antibody-pa1325-1-boster.html2026-03-24T05:03:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1325-1-1-IHC-anti-muscarinic-acetylcholine-receptor-2-antibody.jpgAnti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband&reg;Anti-Muscarinic Acetylcholine Receptor 2 antibody&#44; PA1325-1&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1325-1-chrm2-primary-antibodies-wb-testing-2.jpgAnti-Muscarinic Acetylcholine Receptor 2/CHRM2 Antibody Picoband&reg; Western blot analysis of Muscarinic Acetylcholine Receptor 2 using anti-Muscarinic Acetylcholine Receptor 2 antibody (PA1325-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Muscarinic Acetylcholine Receptor 2 antigen affinity purified polyclonal antibody (Catalog # PA1325-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Muscarinic Acetylcholine Receptor 2 at approximately 71 kDa. The expected band size for Muscarinic Acetylcholine Receptor 2 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ly6al-antibody-pa1327-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1327-1-WB-anti-ly6al-antibody.jpgAnti-Ly6al Antibody Picoband&reg;Anti-Ly6al antibody&#44; PA1327&#44; Western blotting<br>Lane 1: Rat Cardiac Muscle Tissue Lysate<br>Lane 2: Rat Liver Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-n-cadherin-antibody-pa1328-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-wb-testing-1.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;Western blot analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (PA1328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140 kDa. The expected band size for CDH2 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-ihc-testing-1.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;IHC analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-ihc-testing-2_1.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;IHC analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-ihc-testing-3_1.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;IHC analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-ihc-testing-8.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;IHC analysis of CDH2 using anti-CDH2 antibody (PA1328).<br> CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-1.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-8.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-9.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-10.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-11.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-12.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-13.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-14.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-if-testing-15.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; IF analysis of CDH2 using anti-CDH2 antibody (PA1328). <br> CDH2 was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDH2 Antibody (PA1328) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-cdh2-primary-antibodies-fcm-testing-1.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;Flow Cytometry analysis of A549 cells using anti-CDH2 antibody (PA1328). <br> Overlay histogram showing A549 cells stained with PA1328 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CDH2 Antibody (PA1328, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-13045_2018_638_fig4_html.pngAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;The effects of Ube2v1 on epithelial mesenchymal transition and autophagy program in colorectal cancer. a , b Expressions of E-cadherin, β-catenin, Vimentin, Fibronectin, N-cadherin, Twist1, and Snai1were detected after Ube2v1 was overexpressed ( a ) or knocked down ( b ) in DLD-1 and SW480 cells. c Tumor exacted from xenograft model were used to detect expressions of E-cadherin and LC3-II in both DLD-1 and SW480 cells with Ube2v1 stable overexpression by western blotting. d Endogenous LC3 puncta and expressions of E-cadherin, β-catenin, and Vimentin were observed after Ube2v1 was knocked down in SW480cells by immunofluorescent analysis. e Expressions of Ube2v1, ATG5, ATG7,LC3-II, E-cadherin, β-catenin, Vimentin, Fibronectin, and N-cadherin were observed after Ube2v1 was knocked down in HCT116 and SW480 cells with or without knockdown of ATG5 or ATG7 using RNA interference <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13045-018-0638-9'>30016968</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-41598_2022_23837_fig9_html.pngAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;Interfering with DLGAP4 inhibits the PPARβ/δ signalling pathway and the expression of proliferation- and metastasis-related proteins. Western blotting was performed to measure the protein expression of DLGAP4, PPARβ/δ, CyclinD1, E-cadherin and N-cadherin in shNC or shDLGAP4 HepG2 and HCCLM3 cells. The data were obtained from the average of three independent experiments. *P < 0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-022-23837-y'>36396671</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-99188-g006.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;SLC16A8 siRNA reverses the effects of hypoxia on cell endothelial-mesenchymal transition of HUVECs. A and B: The effect of SLC16A8 siRNA on the proliferation of hypoxia treated cells; C and D: SLC16A8 siRNA can affect the migration (C) and invasion (D) of hypoxic HUVECs; E: SLC16A8 siRNA can affect the expression level of E-cadherin, N-cadherin and Vimentin in hypoxia treated cells; F: Effect of SLC16A8 siRNA on the ability of endothelial cells to form tubes after co culture of colorectal cancer cells with hypoxia. a P < 0.05, b P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1328-99188-g003.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg;Effect of hypoxia on endothelial-mesenchymal transition of HUVEC co-cultured with colorectal cancer cells. A and B: Effect of hypoxia on cell viability; C and D: Effect of hypoxia on migration (C) and invasion (D) of HUVECs; E: Effect of hypoxia on the ability of endothelial cells’ tube formation; F: Hypoxia affected the expression of E-cadherin, N-cadherin and Vimentin in HUVECs. a P < 0.05, b P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/c/a/casestudy_pa1328.jpgAnti-N-Cadherin-2 CDH2 CD325-Antibody Picoband&reg; https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nnos-neuronal-antibody-pa1329-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1329-1-WB-anti-nnos-neuronal-antibody.jpgAnti-nNOS (neuronal)/NOS1 Antibody Picoband&reg;Anti-nNOS(neuronal) antibody&#44; PA1329&#44; Western blotting<br>All lanes: Anti nNOS(neuronal)(PA1329) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 160KD<br>Observed bind size: 160KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1329-2-IHC-anti-nnos-neuronal-antibody.jpgAnti-nNOS (neuronal)/NOS1 Antibody Picoband&reg;Anti-nNOS(neuronal) antibody&#44; PA1329&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegfd-antibody-pa1332-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1332-1-WB-anti-vegfd-antibody.jpgAnti-VEGFD/FIGF Antibody Picoband&reg;Anti-VEGFD antibody&#44; PA1332&#44; Western blotting<br>Lane 1: SW620 Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: 6T-CEM Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd34-antibody-pa1334-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-cd34-primary-antibodies-wb-testing-1.jpgAnti-CD34 Antibody Picoband&reg; Western blot analysis of CD34 using anti-CD34 antibody (PA1334). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PA1334) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105 kDa. The expected band size for CD34 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-10020_2025_1178_fig3_html.pngAnti-CD34 Antibody Picoband&reg;H&E ( A – B ) and Trichrome AZAN ( C – D ) stainings of representative endometriotic lesions in DCI-treated mice 28 days p.t. by LM. A , D mag. 10X; bar: 100 µm. B , E mag. 20X; bar: 50 µm. C , F mag. 40X; bar: 20 µm ( E – F ) and Trichrome AZAN ( G , H ) stainings of representative endometriotic lesions in DG + DCI-treated mice 28 days p.t. by LM. E , G mag. 20X; bar: 50 µm. F , H mag. 40X; bar: 20 µm. H&E ( I – K ) and Trichrome AZAN ( J – L ) stainings of representative endometriotic lesions in DG-treated mice 28 days p.t. by LM. I , J mag. 20X; bar: 50 µm. K , L mag. 40X; bar: 20 µm. M – T PCNA immunoreactivity in endometriotic lesions 28 days p.t. of control EMS ( M , N ), DCI-treated ( O , P ), DG + DCI ( Q , R ) and DG-treated ( S , T )-treated mice by LM. M , O , Q , S LM, mag. 20X; bar: 50 µm. N , P , R , T mag. 40X; bar: 20 µm. U – AB CD34 immunoreactivity in endometriotic lesions 28 days p.t. of control EMS ( U , V ), DCI-treated ( W , X ), DG + DCI ( Y , Z ) and DG-treated ( AA , AB )-treated mice by LM. U , W , Y , AA mag. 20X; bar: 50 µm. V , X , Z , AB mag. 40X; bar: 20 µm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01178-6'>40211112</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-10020_2025_1178_fig6_html.pngAnti-CD34 Antibody Picoband&reg;A – D CD34 immunoreactivity in endometriotic ovary 28 days p.t. of control EMS ( A ), DCI ( B ), DG + DCI ( C ) and DG ( D )-treated mice by LM. mag. 20X; bar: 50 µm; E – H ) PCNA immunoreactivity in endometriotic ovary 28 days p.t. of control EMS ( E ), DCI ( F ), DG + DCI ( G ) and DG ( H )-treated mice by LM. mag. 20X; bar: 50 µm. I – L IL-1β immunoreactivity in endometriotic ovary 28 days p.t. of control EMS ( I ), DCI ( J ), DG + DCI ( K ) and DG-treated ( L )-treated mice by LM. mag. 20X; bar: 50 µm. M – P E-Cadherin immunoreactivity in endometriotic ovary 28 days p.t. of control EMS ( M ), DCI ( N ), DG + DCI ( O ) and DG ( P )-treated mice by LM. mag. 20X; bar: 50 µm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01178-6'>40211112</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-ijn-16-7609-g0007.jpgAnti-CD34 Antibody Picoband&reg;Therapeutic effect of NPs composed of PBAE546 and CRISPR/Cas9 recombinant plasmids targeting HPV16 E7 in the vaginas of HPV16 transgenic mice. ( A and B ) Representative images of H&E and IHC staining of the cervical epithelium between the HPV16 transgenic mouse control group and the HPV16 E7 NPs (PBAE546/plasmid 60:1, 10 μg of plasmid per day for 20 days) treatment group. IHC staining indicators included HPV16 E7 , P16 , RB1 , Ki67 , CDK2 , CD34 and E2F1 . Scale bars, 20 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8606985/&orig_host=www.ncbi.nlm.nih.gov'>34819726</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-oncotarget-09-1957-g005.jpgAnti-CD34 Antibody Picoband&reg;Effects of three therapies on angiogenesis, cell proliferation, and apoptosis of SOI tumor. Representative pictures of blood vessels and lymphatic stained with CD34, D2-40, proliferative cells stained with Ki-67, Bcl-2, Bcl-6, and apoptotic cells stained with Tunel antibodies in Control, DOX, PDOX group. Original magnification 40×, treatment with PDOX resulted in decreased Ki-67-positive cells.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5788612/&orig_host=www.ncbi.nlm.nih.gov'>29416744</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-medscimonit-23-3932-g005.jpgAnti-CD34 Antibody Picoband&reg;MTA1 deficiency impairs the formation of pulmonary capillary beds. ( A, B ) Immunohistochemistry results showed fewer CD34-positive cells in the lungs of MTA1 -KO animals compared to wild-type mice at E18.5 and 2M. n=10, Scale bar, 50 μm, * P <0.05, ** P <0.01. ( C ) The percentages of ERG positive cells were similar in the alveolar walls of wild-type and MTA 1-KO mice at E18.5 but were increased significantly in the MTA1-deficient mice of 2M. n=10, Scale bar, 50 μm. ( D ) Western blot analysis highlights the downregulation of CD34 in the lungs of MTA1 -KO mice at E18.5 and 2M compared to wild-type animals. ERG levels were similar at E18.5 but increased significantly in the MTA1-deficient mice of 2M. ( E, F ) The same expression tendencies of CD34 and ERG at mRNA levels were confirmed at the E18.5 and 2M stage by real-time PCR analysis. n=10, * P <0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5567764/&orig_host=www.ncbi.nlm.nih.gov'>28808223</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-ijbsv13p0471g001.jpgAnti-CD34 Antibody Picoband&reg;DPPA inhibits 4T1 subcutaneous tumor growth in vivo . (A) The structure of DPPA. 4T1 cells were injected into the mammary fat pads of BALB/c mice, and 9 days later, DPPA (3 mg/kg body weight) or DMSO was injected once every two days for two weeks. DPPA significantly suppressed tumor volume (B) and tumor weight (C) in a mouse 4T1 subcutaneous tumor model. DPPA inhibited tumor cell proliferation and angiogenesis, which were measured by Ki67 (D) and CD34 (E) staining, respectively, using an IHC assay. N = 8, * P < 0.05, *** P < 0.001. Scale bars: 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5436567/&orig_host=www.ncbi.nlm.nih.gov'>28529455</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-ijbsv13p0471g002.jpgAnti-CD34 Antibody Picoband&reg;DPPA inhibits MDA-MB-231 subcutaneous tumor growth in vivo . MDA-MB-231 cells were injected into the mammary fat pads of athymic nude mice, and 7 days later, DPPA (3 mg/kg body weight) or DMSO was injected once every two days for two weeks. DPPA significantly suppressed tumor volume (A) and tumor weight (B). DPPA inhibited tumor cell proliferation and angiogenesis, which were measured using Ki67 (C) and CD34 (D) staining, respectively, in an IHC assay. N = 8, * P < 0.05, ** P < 0.01 and *** P < 0.001. Scale bars: 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5436567/&orig_host=www.ncbi.nlm.nih.gov'>28529455</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-oncotarget-07-39302-g001.jpgAnti-CD34 Antibody Picoband&reg;Primary culture and characterization of rat EPCs. (A) Rat BM-MNCs from bone marrow after 7 days in culture (100×). (B) Rat BM-MNCs after 14 days of culture (100×). (C) CD31, CD34, CD133 and VEGFR-2 expression was detected using immunofluorescence (200×). (D) EPCs differentiation potential into adipocytes was demonstrated using oil red-O staining (100×). (E) EPCs differentiation potential into smooth muscle cells was demonstrated using immunofluorescence staining (200×). (F) The expression of eNOS in EPCs was identified using Western blotting analyses. Human umbilical vein endothelial cells was used as positive control. Cavernous smooth muscle cells was used as negative control. (G) Tube formation assay was examined microscopically (100×).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5129934/&orig_host=www.ncbi.nlm.nih.gov'>27283992</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-ijbsv10p0404g002.jpgAnti-CD34 Antibody Picoband&reg;Andro inhibits cell proliferation and angiogenesis and induces cell apoptosis in insulinoma. (A) Andro inhibited the tumor cell proliferation, which was measured using the BrdU cell proliferation assay, in RIP1-Tag2 mice. (B) Cell apoptosis was increased, which was examined through TUNEL staining, in Andro treated tumor tissue compared with control groups. (C) and (D) Andro suppressed the tumor angiogenesis, which was measured using the immunohistochemical staining of CD34 and VEGF, in RIP1-Tag2 mice. n=6, * P < 0.05, ** P < 0.01. Bar, 20 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC3979993/&orig_host=www.ncbi.nlm.nih.gov'>24719558</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1334-2-IHC-anti-cd34-antibody.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in a paraffin-embedded section of Rat Kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1334-3-IHC-anti-cd34-antibody.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-cd34-primary-antibodies-ihc-testing-4.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-cd34-primary-antibodies-ihc-testing-5.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-cd34-primary-antibodies-ihc-testing-6.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-7.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-8-ihc-anti-cd34-antibody.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in a frozen section of Rat Placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-9-ihc-anti-cd34-antibody.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PA1334). <br> CD34 was detected in a frozen section of Rat Kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD34 Antibody (PA1334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1334-cd34-primary-antibodies-fcm-testing-10.jpgAnti-CD34 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-CD34 antibody (PA1334). <br> Overlay histogram showing HEL cells stained with PA1334 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD34 Antibody (PA1334, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-19-antibody-pa1335-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1335-2-IHC-anti-cytokeratin-19-antibody.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335). <br> Cytokeratin 19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1335-krt19-primary-antibodies-wb-testing-1_1.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human MDA-MB-453 whole cell lysates,<br> Lane 3: human placenta tissue lysates,<br> Lane 4: human RT4 whole cell lysates,<br> Lane 5: human T-47D whole cell lysates,<br> Lane 6: human A431 whole cell lysates,<br> Lane 7: human HL-60 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 19 antigen affinity purified polyclonal antibody (Catalog # PA1335) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for Cytokeratin 19 at approximately 44 kDa. The expected band size for Cytokeratin 19 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1335-3-IF-anti-cytokeratin-19-antibody.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; ICC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335). <br> Cytokeratin 19 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1335-4.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335).<br> Cytokeratin 19 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cardiac-fabp-antibody-pa1336-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1336-4-IHC-anti-cardiac-fabp-antibody.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband&reg;Cardiac FABP was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-Cardiac FABP Antigen Affinity purified polyclonal antibody (Catalog # PA1336) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1336-2_1-IHC-anti-cardiac-fabp-antibody.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband&reg;Cardiac FABP was detected in paraffin-embedded sections of rat cardiac muscle tissues using rabbit anti-Cardiac FABP Antigen Affinity purified polyclonal antibody (Catalog # PA1336) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1336-3-IHC-anti-cardiac-fabp-antibody.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband&reg;Cardiac FABP was detected in paraffin-embedded sections of mouse skeletal muscletissues using rabbit anti-Cardiac FABP Antigen Affinity purified polyclonal antibody (Catalog # PA1336) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1336-1-WB-anti-cardiac-fabp-antibody.jpgAnti-Cardiac FABP/FABP3 Antibody Picoband&reg; Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (PA1336). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysate&#44; <br> Lane 2: mouse heart tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody (Catalog # PA1336) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grp94-antibody-pa1340-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1340-hsp90b1-primary-antibodies-wb-testing-1.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; Western blot analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (PA1340). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human K562 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP94/HSP90B1 antigen affinity purified polyclonal antibody (Catalog # PA1340) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP94/HSP90B1 at approximately 100 kDa. The expected band size for GRP94/HSP90B1 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1340-2-IHC-anti-grp94-antibody.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; ICC analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (PA1340). <br> GRP94/HSP90B1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-GRP94/HSP90B1 Antibody (PA1340) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1340-3-IHC-anti-grp94-antibody.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; IHC analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (PA1340). <br> GRP94/HSP90B1 was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GRP94/HSP90B1 Antibody (PA1340) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1340-4-IHC-anti-grp94-antibody.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; IHC analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (PA1340). <br> GRP94/HSP90B1 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GRP94/HSP90B1 Antibody (PA1340) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1340-grp94-primary-antibodies-wb-testing-5.pngAnti-GRP94/HSP90B1 Antibody Picoband&reg; Western blot analysis of GRP94/HSP90B1 using anti-GRP94/HSP90B1 antibody (PA1340). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP94/HSP90B1 antibody (PA1340) at 1:2000 in TBST/5% BSA overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP94/HSP90B1 at approximately 92 kDa. The expected band size for GRP94/HSP90B1 is at 92 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-1-beta-antibody-pa1341-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1341-1-WB-anti-il-1-beta-antibody.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;Anti-IL-1 beta antibody&#44; PA1341&#44; Western blotting<br>Lane 1: Recombinant Human IL-1 beta Protein 20ng<br>Lane 2: Recombinant Human IL-1 beta Protein 10ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-18-antibody-pa1342-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1342-1-WB-anti-il-18-antibody.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Anti-IL-18 antibody&#44; PA1342&#44; Western blotting<br>Lane 1: Recombinant Human IL-18 Protein 10ng<br>Lane 2: Recombinant Human IL-18 Protein 5ng<br>Lane 3: Recombinant Human IL-18 Protein 2.5ng<br> Lane 4: RAJI Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1342-2-WB-anti-il-18-antibody.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Western blot analysis of IL-18 expression in HELA whole cell lysates (lane 1). IL-18 at 22KD was detected using rabbit anti-IL-18 Antigen Affinity purified polyclonal antibody (Catalog # PA1342) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1342-41419_2023_6068_fig2_html.pngAnti-Interleukin-18 IL18 Antibody Picoband&reg;Melanoma specimens reduce PRGs positive cells in epidermal clinically. A Overall survival analysis based on K-M was calculated to identify the potential PRGs clinically. B PPI via MCODE and CytoNCA algorithm was established, in which PRGs were screened out as central protein functioning in melanoma deficiency. C GZMA, GSDMB, CHMP4A, NLRP1, and IL18 protein expression of melanoma and control patients detected by ELISA. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n = 5, compared with the normal group. D Representative IHC stanning of GZMA + , GSMDB + , CHMP4A + , NLRP1 + , and IL18 + in melanoma specimens from the clinic. Scale bar = 100 μm, n = 5 samples per group. E The quantification of PRGs protein in epidermal areas, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared with the control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-023-06068-5'>37620327</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1342-41419_2023_6068_fig5_html.pngAnti-Interleukin-18 IL18 Antibody Picoband&reg;PRGs were primarily expressed in immune cells. A Feature plots depicting the expression of key pyroptosis, violin plots were also displayed to determine the cell type. B Representative IHC stanning of GZMA + , GSMDB + , CHMP4A + , NLRP1 + , and IL18 + in melanoma specimens, especially in lymphocyte areas. Scale bar = 100 μm, n = 4 samples per group. C The quantification of PRGs protein in dermal areas, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared with the control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-023-06068-5'>37620327</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erk1-antibody-pa1343-boster.html2026-03-24T05:03:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1343-1-WB-anti-erk1-antibody.jpgAnti-ERK1/MAPK3 Antibody Picoband&reg;Anti-ERK1 antibody&#44; PA1343&#44; Western blotting<br>Lane 1: Rat Spleen Tissue Lysate<br>Lane 2: Rat Thymus Tissue Lysate<br>Lane 3: Rat Skeletal Muscle Tissue Lysate<br>Lane 4: Rat Kidney Tissue Lysate<br>Lane 5: HELA Cell Lysate<br>Lane 6: JURKAT Cell Lysate<br>Lane 7: RAJI Cell Lysate<br>Lane 8: COLO320 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1343-2-IHC-anti-erk1-antibody.jpgAnti-ERK1/MAPK3 Antibody Picoband&reg;Anti-ERK1 antibody&#44; PA1343&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1343-3-IHC-anti-erk1-antibody.jpgAnti-ERK1/MAPK3 Antibody Picoband&reg;Anti-ERK1 antibody&#44; PA1343&#44; IHC(P)<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1343-4.jpgAnti-ERK1/MAPK3 Antibody Picoband&reg; IHC analysis of ERK1 using anti-ERK1 antibody (PA1343). <br> ERK1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ERK1 Antibody (PA1343) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-upa-receptor-antibody-pa1344-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1344-plaur-primary-antibodies-wb-testing-1.jpgAnti-uPA Receptor/PLAUR Antibody Picoband&reg; Western blot analysis of PLAUR using anti-PLAUR antibody (PA1344). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAUR antigen affinity purified polyclonal antibody (Catalog # PA1344) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAUR at approximately 45-55 kDa. The expected band size for PLAUR is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1344-plaur-primary-antibodies-ihc-testing-2.jpgAnti-uPA Receptor/PLAUR Antibody Picoband&reg; IHC analysis of PLAUR using anti-PLAUR antibody (PA1344). <br> PLAUR was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLAUR Antibody (PA1344) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sod1-antibody-pa1345-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-sod1-primary-antibodies-wb-testing-1.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg;Western blot analysis of SOD1 using anti-SOD1 antibody (PA1345). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MDA-MB-453 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat spleen tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse spleen tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PA1345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-fphar-09-00497-g005.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg;Effects of acacetin on antioxidant-related proteins in cells with hypoxia/reoxygenation (H/R) exposure. Western blots and mean relative levels of Nrf2 (A) , HO-1 (B) , SOD1 (C) , SOD2 (D) in neonatal rat cardiomyocytes without (control) or with hypoxia/reoxygenation exposure in the absence (V, vehicle) or presence of 0.3, 1, or 3 μM acacetin. Western blots and mean relative levels of Nrf2 (E) , HO-1 (F) , SOD1 (G) , SOD2 (H) in H9C2 cardiomyoblasts without (control) or with hypoxia/reoxygenation exposure in the absence or presence of 0.3, 1, or 3 μM acacetin. Data were expressed as mean ± SEM and analyzed by one-way ANOVA followed by Bonferroni-test ( n = 5 individual experiments, ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. hypoxia/reoxygenation alone).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00497/full'>29867499</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-fphar-09-00497-g007.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg;Effects of silencing Nrf2 on antioxidant proteins in cells with hypoxia/reoxygenation insult. (A) Western blots and relative levels of Nrf2 in H9C2 cardiomyoblasts transfected with control siRNA or Nrf2 siRNA and subjected to hypoxia/reoxygenation insult in the absence (V, vehicle) or presence of 3 μM acacetin (Aca). (B) Western blots and relative levels of HO-1 in H9C2 cardiomyoblasts with the treatment used in (A) . (C) Western blots and relative levels of SOD1 in H9C2 cardiomyoblasts with the treatment used in (A) . (D) Western blots and relative levels of SOD2 in H9C2 cardiomyoblasts with the treatment used in (A) . Data were expressed as mean ± SEM and analyzed by one-way ANOVA followed by Bonferroni-test ( n = 5 individual experiments, ∗ P < 0.05, ∗∗ P < 0.01 vs. vehicle of control siRNA; ## P < 0.01 vs. control siRNA with acacetin).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00497/full'>29867499</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-sod1-primary-antibodies-ihc-testing-2.jpg.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg; IHC analysis of SOD1 using anti-SOD1 antibody (PA1345). <br> SOD1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD1 Antibody (PA1345) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-sod1-primary-antibodies-ihc-testing-3.jpg.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg; IHC analysis of SOD1 using anti-SOD1 antibody (PA1345). <br> SOD1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD1 Antibody (PA1345) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-sod1-primary-antibodies-ihc-testing-4.jpg.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg; IHC analysis of SOD1 using anti-SOD1 antibody (PA1345). <br> SOD1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD1 Antibody (PA1345) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-sod1-primary-antibodies-ihc-testing-5.jpg.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg; IHC analysis of SOD1 using anti-SOD1 antibody (PA1345). <br> SOD1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD1 Antibody (PA1345) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-sod1-primary-antibodies-ihc-testing-6.jpg.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg; IHC analysis of SOD1 using anti-SOD1 antibody (PA1345). <br> SOD1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD1 Antibody (PA1345) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1345-sod1-primary-antibodies-if-testing-7.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg; IF analysis of SOD1 using anti-SOD1 antibody (PA1345). <br> SOD1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SOD1 Antibody (PA1345) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sftpa1-antibody-pa1347-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1347-1_1.jpgAnti-SFTPA1 Antibody Picoband&reg; Western blot analysis of SFTPA1 using anti-SFTPA1 antibody (PA1347). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody (Catalog # PA1347) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26-35KD. The expected band size for SFTPA1 is at 26KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a2-antibody-pa1348-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1348-anxa2-primary-antibodies-wb-testing-1.jpgAnti-Annexin A2/ANXA2 Antibody Picoband&reg;Western blot analysis of ANXA2 using anti-ANXA2 antibody (PA1348). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human placenta tissue lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse kidney tissue lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA2 antigen affinity purified polyclonal antibody (PA1348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ANXA2 at approximately 36 kDa. The expected band size for ANXA2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1348-anxa2-primary-antibodies-ihc-testing-1.jpgAnti-Annexin A2/ANXA2 Antibody Picoband&reg;IHC analysis of ANXA2 using anti-ANXA2 antibody (PA1348). <br> ANXA2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANXA2 Antibody (PA1348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1348-anxa2-primary-antibodies-fcm-testing-1.jpgAnti-Annexin A2/ANXA2 Antibody Picoband&reg;Flow Cytometry analysis of Hela cells using anti-ANXA2 antibody (PA1348). <br> Overlay histogram showing Hela cells stained with PA1348 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ANXA2 Antibody (PA1348, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdac2-antibody-pa1350-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1350-hdac2-primary-antibodies-wb-testing-1.jpgAnti-Histone deacetylase 2 HDAC2 Antibody Picoband&reg; Western blot analysis of HDAC2 using anti-HDAC2 antibody (PA1350). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human THP-1 whole cell lysates, <br> Lane 3: rat brian tissue lysates, <br> Lane 4: rat lung tissue lysates, <br> Lane 5: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC2 antigen affinity purified polyclonal antibody (Catalog # PA1350) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC2 at approximately 60 kDa. The expected band size for HDAC2 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-1-beta-antibody-pa1351-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1351-1-WB-anti-il-1-beta-antibody.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;Anti-IL-1 beta antibody&#44; PA1351&#44; Western blotting<br>Lane 1: Recombinant Mouse IL-1 beta Protein 10ng<br>Lane 2: Recombinant Mouse IL-1 beta Protein 5ng<br>Lane 3: Recombinant Mouse IL-1 beta Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-6-antibody-pa1352-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-gr5.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Validation of PPD animal models by evaluating uterine inflammation (n = 8). (A) HE staining of the uterus from the female mice (200 × ); (B) Mean endometrial thickness; (C) Immunohistochemical staining of the uterus (400 × ); AOD values of (D) IL-6 and (E) TNF-α in the uterus. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)11394-1'>39166014</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-dddt_a_12305505_f0004_c.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;(A) Immunohistochemical images of IL-1β, IL-6, TNF-α, and NF-κB; Mean density: (B) IL-1β; (C) IL-6; (D) TNF-α; (E) NF-κB; (F) Western blot of IL-1β, IL-6, and TNF-α expression; (G) Peripheral blood IL-1β content. Data are presented as mean ± SD. Vs NC, ##P <0.01; Vs PCOS, *P <0.05, **P <0.01; n = 6 per group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/DDDT.S484531'>39247793</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-12951_2025_3634_fig9_html.pngAnti-Interleukin-6 IL6 Antibody Picoband&reg;( a ) Hematoxylin-eosin (H&E). Immunohistochemical analysis for CD68, TNF-α, and MCP-1 in the kidney samples from each group. ( b ) Periodic acid-Schiff (PAS) staining was conducted, in addition to immunohistochemical analysis for IL-6, MPO, and Caspase-3 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03634-1'>40855423</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-40860947.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Histological evaluation and statistical analysis of diabetic wound after Mn3O4 treatment by immunohistochemical staining. Immunohistochemical staining of (A) IL-6, (B) CD86 and (C) CD206 in the wound bed at day 7. (D–F) Statistical results of immunohistochemical staining in each group. Data are presented as mean ± SD. Statistical significance was determined using t-test (n = 3, ns = no significance, ***P < 0.001, ****P < 0.0001).<br><b>Index in Regenerative Biomaterials under a CC BY license. DOI: <a href='https://academic.oup.com/rb/advance-article-abstract/doi/10.1093/rb/rbaf089/8240290'>10.1093/rb/rbaf089</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-kvir_a_2384564_f0004_oc.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;PRRSV infection induced testicular inflammatory responses. (a) TSI changes in each group. (b) Rectal and testicular temperature changes in each group from 1 to 30 dpc. (c) The expression levels of IFN-γ, TNF-α, IL-6, IL-1α, IL-1β, and IL-10 in the testes were measured via ELISA analysis. ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns, not significant. (d) IHC staining for IFN-γ in porcine testis tissue sections. Scale bars = 100 μm. (e) IHC staining for IL-6 in porcine testis tissue sections. Scale bars = 100 μm. (f) GO-enrichment analysis of dif-mRNAs associated with inflammation. The top 20 GO pathways are shown. (g) KEGG enrichment analysis of dif-mRNAs associated with inflammation. The top 15 KEGG pathways are shown. The sizes and colours of the solid circles represent the number of enriched dif-mRNAs and the significance of the enrichment, respectively. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.1080/21505594.2024.2384564'>39072452</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-research.0445.fig.006.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Collagen deposition, inflammation, and angiogenesis analyses in acute large-area wounds. (A) Masson staining and immunofluorescence of IL-6 (red) and CD31 (red) and a-SMA (green) in wound samples. Scale bars: 100 μm (top) and 50 μm (middle and bottom). (B to D) Quantification assessment of (B) collagen deposition, (C) IL-6 expression, and (D) CD31 expression. **P < 0.01, ***P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://spj.science.org/doi/full/10.34133/research.0445'>39109247</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-il6-primary-antibodies-wb-testing-1.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Western blot analysis of IL6 using anti-IL6 antibody (PA1352). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant rat IL6 protein 5 ng.<br> Lane 2: recombinant rat IL6 protein 10 ng.<br> Lane 3: recombinant rat IL6 protein 0.5 ng.<br> Lane 4: recombinant rat IL6 protein 0.1 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-il6-primary-antibodies-wb-testing-2.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Western blot analysis of IL6 using anti-IL6 antibody (PA1352). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 28-30 kDa. The expected band size for IL6 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-il6-primary-antibodies-wb-testing-3.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Western blot analysis of IL6 using anti-IL6 antibody (PA1352). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: rat spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 28-30 kDa. The expected band size for IL6 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-il6-primary-antibodies-wb-testing-4.pngAnti-Interleukin-6 IL6 Antibody Picoband&reg;Western blot analysis of IL6 using anti-IL6 antibody (PA1352). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: 2h drug treated-human MCF-7 whole cell lysates,<br> Lane 3: 4h drug treated-human MCF-7 whole cell lysates,<br> Lane 4: 12h drug treated-human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1352) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:2000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system system. A specific band was detected for IL6 at 24 kDa. The expected band size for IL6 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-il6-primary-antibodies-ihc-testing-1.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;IHC analysis of IL6 using anti-IL6 antibody (PA1352). <br>IL6 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL6 Antibody (PA1352) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-fneur-12-750908-g004.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Relative expression of IL-6, caspase-3, and cleaved-caspase-3. (A) Immunoblot results of IL-6, caspase-3, and cleaved-caspase-3. (B) Analysis of IL-6 relative expression. One-way ANOVA showed differences among the five groups, F = 10.86, p < 0.0001. (C) Analysis of caspase-3 relative expression. One-way ANOVA showed differences among the five groups, F = 8.50, p = 0.0004. (D) Analysis of cleaved-caspase-3 relative expression. One-way ANOVA showed differences among the five groups, F = 17.36, p < 0.0001. ANOVA, analysis of variance; caspase-3, cysteinyl aspartate specific proteinase 3; IL-6, interleukin 6.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2021.750908/full'>34975719</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-fneur-12-750908-g001.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Experimental workflow. One hundred four rats were randomly divided into five groups: group S (sham, n = 20), group M (middle cerebral artery occlusion [MCAO], n = 28), group H2M (intermittent hypobaric hypoxia preconditioned MCAO group, 2 h/day, n = 20), group H6M (intermittent hypobaric hypoxia preconditioned MCAO group, 6 h/day, n = 28), and group HpM (persistent hypobaric hypoxia preconditioned MCAO group, n = 28). Behavioral tests and morphological staining (TTC staining) were used to analyze the severity of infarction. Total protein expression of NeuN (a specific marker of mature neurons), caspase-3, cleaved-caspase-3, and IL-6 was estimated using western blotting, which explained the severity of injury from different perspectives. Ultrastructural changes were observed under a transmission electron microscope. The most effective pretreatment group was selected for further label-free proteomic study and provided a reliable direction for mechanism exploration. Western blotting was used to verify the expression of the target protein, and key markers for the biological process were detected using immunofluorescence. caspase-3, cysteinyl aspartate specific proteinase 3; IL-6, interleukin 6; NeuN, neuron-specific nuclear protein; TTC, 2,3,5-triphenyl tetrazolium chloride.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2021.750908/full'>34975719</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-41536_2022_254_fig6_html.pngAnti-Interleukin-6 IL6 Antibody Picoband&reg;Inhibitive effect of nanofiber membranes on anti-inflammation in Il-1β induced chondrocytes. a Relative mRNA expression levels of Il6, Tnf-a, iNos, Mmp1, Mmp3, and Mmp13 in IL-1β induced chondrocytes cultured on P, PGF, PF, PPF, or PPGF nanofiber membranes. b Protein expression of MMP13, TNF-a, and IL6 in IL-1β induced chondrocytes cultured on P, PGF, PF, PPF, or PPGF nanofiber membranes (Scale bars, 100 µm). The values were presented as mean ± SD ( n = 3; statistics: one-way ANOVA; # means p < 0.01, ** , ## means p < 0.01, *** , ### means p < 0.001, * is the statistical difference compare with P group and # is the statistical difference between the pairwise comparison). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41536-022-00254-3'>36323709</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-fphar-15-1430599-g003.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;MT inhibited the production of pro-inflammatory proteins in sleep-deprived rats. (A) Western blot bands showing the protein expression levels of IL-1β, IL-6, TNF-α, iNOS, and COX2 in the HP, respectively. (B–F) Relative protein expression level of IL-1β, IL-6, TNF-α, iNOS, and COX2 in the HP, respectively. The data are expressed as the means ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001 vs Control group; * p < 0.05, ** p < 0.01 vs. Model group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1430599/full'>39101143</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-thnov15p6553g008.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Therapeutic effectiveness evaluated after administering AE@SiO 2 -MTX. (A) Knee joints were stained with HE and safranin O. Scale bar: 100 μm. (B) TNF-α, IL-6, MMP3 and MMP13 and DAPI staining applied to knee sections. (C) Line profile analysis verified the co-expression and co-localization of TNF-α and IL-6, MMP3 and MMP13. Scale bar: 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12160022/'>40521182</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-thnov15p6553g007.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Intravenous administration of AE@SiO 2 -MTX alleviated arthritis symptoms in CIA mice. (A) Visual outline of the in vivo treatment procedure. (B-D). Quantification of paw thickness, paw volume and arthritis score at multiple intervals after treatments. (E) Illustrative images of hindlimbs from each group before and after treatments. (F) Micro-CT images in 3D of fore paws, hind paws, and knee joints for different treatment groups. (G-H) The evaluation of BMD levels in six groups post-treatments. (I-J) Plasma levels of IL-6 and IL-10 after treatments. Data were expressed as mean±SD. Data were analyzed for statistical significance via One-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12160022/'>40521182</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1352-13287_2022_2980_fig7_html.pngAnti-Interleukin-6 IL6 Antibody Picoband&reg;PF-127/hADSCs-Exos complex treatment inhibits inflammatory reaction. a Representative images of TNF-α immunostaining at 4, 7, and 10 days after treatment. Scale bar = 20 µm. b Quantification of TNF-α + IHC stained tissues. c Representative images illustrating IHC results of IL-6 at 4, 7, and 10 days after surgery. Scale bar = 20 µm. d Quantification of IL-6 + IHC stained tissues. e IHC images of wound sections stained with CD68 on days 4, 7, and 10 post-wounding. Scale bar = 20 µm. f Quantification of the number of CD68 positive cells in the wound area on days 4, 7, and 10. g IHC images of wound sections stained with CD206 at days 4, 7, and 10 post-wounding. Scale bar = 20 µm. h Quantification of the number of CD206 positive cells in the wound area on days 4, 7, and 10. In b, d, and f , data are shown as mean ± SEM; n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus vehicle control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-022-02980-3'>35941707</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-8-antibody-pa1353-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1353-il8-primary-antibodies-wb-testing-1.jpgAnti-IL8/CXCL8 Antibody Picoband&reg; Western blot analysis of IL8 using anti-IL8 antibody (PA1353). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human IL8 protein 10ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL8 antigen affinity purified polyclonal antibody (Catalog # PA1353) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 11 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1353-il8-primary-antibodies-ihc-testing-2.jpgAnti-IL8/CXCL8 Antibody Picoband&reg; IHC analysis of IL8 using anti-IL8 antibody (PA1353). <br> IL8 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL8 Antibody (PA1353) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-10-antibody-pa1354-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1354-1-WB-anti-il-10-antibody.jpgAnti-Interleukin-10 IL10 Antibody Picoband&reg;Anti-IL-10 antibody&#44; PA1354&#44; Western blotting<br>Lane 1: Recombinant Human IL-10 Protein 10ng<br>Lane 2: Recombinant Human IL-10 Protein 5ng <br>Lane 3: Recombinant Human IL-10 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp-9-antibody-pa1357-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1357-1-WB-anti-mmp-9-antibody.jpgAnti-Matrix metalloproteinase-9 MMP9 Antibody Picoband&reg;Anti-MMP-9 antibody&#44; PA1357&#44; Western blotting<br>Lane 1: Rat Embryo Tissue Lysate<br>Lane 2: MM453 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: SMMC Cell Lysate<br>Lane 5: JURKAT Cell Lysate<br>Lane 6: HT1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-presenilin-2-antibody-pa1358-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1358-1-WB-anti-presenilin-2-antibody.jpgAnti-Presenilin 2/PSEN2 Antibody Picoband&reg;Anti-Presenilin 2 antibody&#44; PA1358&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate <br>Lane 2: Rat Brain Tissue Lysate <br>Lane 3: MCF-7 Cell Lysate <br>Lane 4: HELA Cell Lysate <br>Lane 5: SMMC Cell Lysate <br>Lane 6: CEM Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1358-2-IHC-anti-presenilin-2-antibody.jpgAnti-Presenilin 2/PSEN2 Antibody Picoband&reg;Anti-Presenilin 2 antibody&#44; PA1358&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1358-4-IHC-anti-presenilin-2-antibody.jpgAnti-Presenilin 2/PSEN2 Antibody Picoband&reg;Anti-Presenilin 2 antibody&#44; PA1358&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1358-3-IHC-anti-presenilin-2-antibody.jpgAnti-Presenilin 2/PSEN2 Antibody Picoband&reg;Anti-Presenilin 2 antibody&#44; PA1358&#44; IHC(P)<br>IHC(P): Rat Kidney Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-l-selectin-antibody-pa1359-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1359-1_1-WB-anti-l-selectin-antibody.jpgAnti-CD62L/SELL Antibody Picoband&reg;Anti-L-selectin antibody&#44; PA1359&#44; Western blotting<br>All lanes: Anti L-selectin (PA1359) at 0.5ug/ml<br> Lane 1: Rat Spleen Tissue Lysate at 50ug<br> Lane 2: Mouse Spleen Tissue Lysate at 50ug<br> Predicted bind size: 42KD<br> Observed bind size: 70KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tgf-alpha-antibody-pa1360-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1360-1-WB-anti-tgf-alpha-antibody.jpgAnti-TGF alpha/TGFA Antibody Picoband&reg;Anti-TGF alpha antibody&#44; PA1360&#44; Western blotting<br>All lanes: Anti TGF alpha (PA1360) at 0.5ug/ml<br>Lane 1: Recombinant Human TGF a Protein 10ng<br>Lane 2: Recombinant Human TGF a Protein 5ng<br>Lane 3: Recombinant Human TGF a Protein 2.5ng<br>Predicted bind size: 6KD<br>Observed bind size: 6KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-beta-antibody-pa1361-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1361-1-WB-anti-tnf-beta-antibody.jpgAnti-TNF beta/LTA Antibody Picoband&reg;Anti-TNF beta antibody&#44; PA1361&#44; Western blotting<br>Lane 1: Recombinant Human TNF beta Protein 10ng<br>Lane 2: Recombinant Human TNF beta Protein 5ng<br>Lane 3: Recombinant Human TNF beta Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p-cadherin-antibody-pa1363-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1363-cdh3-primary-antibodies-wb-testing-1.jpgAnti-P-Cadherin-3 CDH3-Antibody Picoband&reg; Western blot analysis of CDH3 using anti-CDH3 antibody (PA1363). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hacat whole cell lysates,<br> Lane 2: human A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH3 antigen affinity purified polyclonal antibody (Catalog # PA1363) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH3 at approximately 120 kDa. The expected band size for CDH3 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1363-2-IHC-anti-p-cadherin-antibody.jpgAnti-P-Cadherin-3 CDH3-Antibody Picoband&reg; IHC analysis of CDH3 using anti-CDH3 antibody (PA1363). <br> CDH3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CDH3 Antibody (PA1363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1363-3-IF-anti-p-cadherin-antibody.jpgAnti-P-Cadherin-3 CDH3-Antibody Picoband&reg; IHC analysis of CDH3 using anti-CDH3 antibody (PA1363). <br> CDH3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CDH3 Antibody (PA1363) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calpain-1-antibody-pa1364-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1364-capn1-primary-antibodies-wb-testing-1.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; Western blot analysis of CAPN1 using anti-CAPN1 antibody (PA1364). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human RT4 whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPN1 antigen affinity purified polyclonal antibody (Catalog # PA1364) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAPN1 at approximately 82 kDa. The expected band size for CAPN1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1364-2-IHC-anti-calpain-1-antibody.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; IHC analysis of Calpain 1 using anti-Calpain 1 antibody (PA1364). <br> Calpain 1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpain 1 Antibody (PA1364) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1364-3-IF-anti-calpain-1-antibody.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; IHC analysis of Calpain 1 using anti-Calpain 1 antibody (PA1364). <br> Calpain 1 was detected in immunocytochemical section of HELA Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Calpain 1 Antibody (PA1364) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1364-4-IHC-anti-calpain-1-antibody.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; IHC analysis of Calpain 1 using anti-Calpain 1 antibody (PA1364). <br> Calpain 1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpain 1 Antibody (PA1364) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1364-5.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; IHC analysis of Calpain 1 using anti-Calpain 1 antibody (PA1364). <br> Calpain 1 was detected in frozen section of rat cardiac muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calpain 1 Antibody (PA1364) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1364-capn1-primary-antibodies-if-testing-6.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; IF analysis of CAPN1 using anti- CAPN1 antibody (PA1364). <br> CAPN1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CAPN1 Antibody (PA1364) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd80-antibody-pa1365-boster.html2026-03-24T05:03:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1365-cd80-primary-antibodies-wb-testing-1_1.jpgAnti-CD80 Antibody Picoband&reg; Western blot analysis of CD80 using anti-CD80 antibody (PA1365). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human Daudi whole cell lysates,<br> Lane 3: human Ramos whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD80 antigen affinity purified polyclonal antibody (Catalog # PA1365) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD80 at approximately 70 kDa. The expected band size for CD80 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1365-cd80-primary-antibodies-wb-testing-2.jpgAnti-CD80 Antibody Picoband&reg; Western blot analysis of CD80 using anti-CD80 antibody (PA1549). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD80 antigen affinity purified polyclonal antibody (Catalog # PA1549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD80 approximately 70 kDa. The expected band size for CD80 is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd80-antibody-pa1365-1-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1365-1-1-WB-anti-b7-1-cd80-antibody.jpgAnti-CD80 Antibody Picoband&reg;Anti-CD80 antibody&#44; PA1365-1&#44; Western blotting<br>All lanes: Anti CD80 (PA1365-1) at 0.5ug/ml<br>WB: Rat Thymus Tissue Lysate at 50ug<br>Predicted bind size: 34KD<br>Observed bind size: 50KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc42-antibody-pa1366-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1366-1-WB-anti-cdc42-antibody.jpgAnti-CDC42 Antibody Picoband&reg;Anti-CDC42 antibody&#44; PA1366&#44; Western blotting<br>Lane 1: Recombinant Human CDC42 Protein 10ng<br>Lane 2: Recombinant Human CDC42 Protein 5ng<br>Lane 3: Recombinant Human CDC42 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1366-2-IHC-anti-cdc42-antibody.jpgAnti-CDC42 Antibody Picoband&reg;Anti-CDC42 antibody&#44; PA1366&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-connexin-32-gjb1-antibody-pa1367-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1367-1-WB-anti-connexin-32-gjb1-antibody.jpgAnti-Connexin 32/GJB1 Antibody Picoband&reg;Anti-Connexin 32/GJB1 antibody&#44; PA1367&#44; Western blotting<br>Lane 1: Rat Cardiac Muscle Tissue Lysate<br>Lane 2: Rat Cardiac Muscle Tissue Lysate<br>Lane 3: Rat Skeletal Muscle Tissue Lysate<br>Lane 4: Rat Brain Tissue Lysate<br>Lane 5: MCF-7 Cell Lysate<br>Lane 6: HELA Cell Lysate<br> Lane 7: SMMC Cell Lysate<br> Lane 8: JURKAT Cell Lysate<br> Lane 9: COLO320 Cell Lysate https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1367-2-IHC-anti-connexin-32-gjb1-antibody.jpgAnti-Connexin 32/GJB1 Antibody Picoband&reg;Anti-Connexin 32/GJB1 antibody&#44; PA1367&#44; IHC(P)<br>IHC(P):Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-gjb1-primary-antibodies-ihc-testing-3.jpgAnti-Connexin 32/GJB1 Antibody Picoband&reg;IHC analysis of Connexin-32/GJB1 using anti-Connexin-32/GJB1 antibody (PA1367). <br>Connexin-32/GJB1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Connexin-32/GJB1 Antibody (PA1367) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig1_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Expression and distribution of Cx32, Cx26 and Cx43 in patients with HCC. a. The protein expression levels of Cx32, Cx26 and Cx43 were determined by western blot analysis. β-actin was used as the loading control. b. The expression of Cx32 was correlated with increased TNM stages, as revealed by western blot analysis. β-actin was used as the loading control. c . Statistical analysis of the relative expression levels of Cxs in HCC tissues, peritumoral tissues, and normal liver tissues. ** , P < 0.01; ## , P < 0.01. d. Statistical analysis of the relative expression levels of Cx32 in peritumoral tissues and HCC tissues with different TNM stages. * , P < 0.05. e. Representative IHC staining of Cx32, Cx26 and Cx43 protein in normal liver tissues (left panels), peritumoral tissues (middle panels) and HCC tissues (right panels) (400×). Scale bars: 50 μm. f. Representative IHC staining of Cx32 in normal liver tissues, cirrhotic tissues and early and advanced HCC tissues (400×). Scale bars: 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig2_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Kaplan-Meier analysis. Patients in the low Cx32 group ( n = 48) had significantly longer overall survival (OS) times than those in the high Cx32 group ( n = 48) ( P = 0.014, log-rank test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig3_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Cx32 regulates the expression of Bcl-2 family proteins in HCC cell lines. a. Cx32 expression was knocked down in HepG2 cells by siRNA transfection. siCx32_2 showed the greatest efficiency in reducing Cx32 expression. b. Transient plasmid transfection into SMMC-7721 cells induced Cx32 overexpression. c. Silencing Cx32 expression in HepG2 cells increased the expression levels of Bax and Bak but decreased the expression level of Bcl-2 ( n = 3). ** , P < 0.01 versus NC. d. Overexpression of Cx32 in SMMC-7721 cells caused the upregulation of Bcl-2 expression and the downregulation of Bax and Bak expression (n = 3). ** , P < 0.01 versus Vector <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig4_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Cx32 exerts an anti-apoptotic effect on HepG2 cells in a GJ-independent manner. a. When GJ function was inhibited by pretreatment with 2-APB (50 μm, 2 h), knockdown of Cx32 promoted the SN-induced increase in the levels of cleaved-caspase3 and cleaved-PRAR in HepG2 cells, as revealed by western blot analysis (n = 3). ** , P < 0.01; ## , P < 0.01. b. Silencing the expression of Cx32 promoted SN-induced apoptosis in HepG2 cells when GJ function was inhibited by 2-APB, as assessed by flow cytometry (n = 3). ## , P < 0.01 versus NC; ** , P < 0.01 versus NC + 2-APB + SN. c. When GJs were physically eliminated by low-density culture conditions, Cx32 silencing enhanced the SN-induced increase in the levels of cleaved-caspase3 and cleaved-PARP in HepG2 cells, as revealed by western blot analysis (n = 3). ** , P < 0.01; ## , P < 0.01. d. Cx32 silencing facilitated SN-induced apoptosis in HepG2 cells in low-density culture, as assessed by flow cytometry (n = 3). ** , P < 0.01 versus NC + SN <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig5_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Overexpressed Cx32 exerts an anti-apoptotic effect on SMMC-7721 cells in a GJ-independent manner. a. When GJ function was inhibited by pretreatment with 2-APB, overexpression of Cx32 alleviated the SN-induced increase in the levels of cleaved-caspase3 and cleaved-PARP in SMMC-7721 cells, as revealed by western blot analysis (n = 3). ** , P < 0.01; ## , P < 0.01. b. Upregulation of Cx32 expression suppressed SN-induced apoptosis in SMMC-7721 cells when GJ function was inhibited by 2-APB, as revealed by flow cytometry ( n = 3). ** , P < 0.01 versus Cx32 + 2-APB + SN. c. When GJ were physically eliminated by low-density culture conditions, Cx32 overexpression reduced SN-induced increase in the levels of cleaved-caspase3 and cleaved-PARP in SMMC-7721 cells ( n = 3). ** , P < 0.01; ## , P < 0.01. d. Upregulation of Cx32 expression inhibited SN-induced apoptosis in SMMC-7721 cells in low-density culture, as revealed by flow cytometry ( n = 3). ** , P < 0.01 versus Cx32 + SN <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig6_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Cx32 exerts anti-apoptotic effects by activating EGFR signaling pathway. a. In 30 HCC specimens, the expression of EGFR was significantly correlated with the expression of Cx32 ( r = 0.662, P < 0.01) . b. The expression level of EGFR was significantly higher in HepG2 cells than in SMMC-7721 cells. c and d. The effects of Cx32 knockdown or overexpression on the EGFR signaling pathway in HepG2 cells and SMMC-7721 cells were determined by western blot analysis ( n = 3, respectively). ** , P < 0.01 versus HepG2 NC ( c ) or SMMC-7721 Vector ( d ). e . In rescue experiments, cotransfection of siRNA-Cx32 and EGFR-expression vectors into HepG2 cells reversed the pro-apoptotic effects of Cx32 knockdown. ** , P < 0.01; ## , P < 0.01. f. In rescue experiments, cotransfection of Cx32 expression vectors and siRNA-EGFR into SMMC-7721 cells reversed the anti-apoptotic effects of Cx32 overexpression. ** , P < 0.01; ## , P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig7_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Cx32 upregulates the expression and activation of EGFR by binding with Src. a and b. In HCC cell lines, silencing or overexpressing Cx32 caused Src downregulation or upregulation, respectively. ** , P < 0.01 versus HepG2 NC ( a ) or SMMC-7721 Vector ( b ). c . Silencing Cx32 by siCx32 transfection caused a decrease in the EGFR and Src mRNA levels in HepG2 cells, as determined by qPCR. GAPDH was used as the loading control. ** , P < 0.01 versus NC. d . Overexpression of Cx32 in SMMC-7721 cells upregulated the mRNA levels of Cx32 (left panel), EGFR and Src (right panel), as determined by qPCR. GAPDH was used as the loading control. ** , P < 0.01 versus Vector. e . The decrease in the levels of EGFR, p-EGFR and Src mediated by siCx32 was reversed by cotransfection of the Src overexpression vector in HepG2 cells. ** , P < 0.01 versus NC. f. The increase in the levels of EGFR, p-EGFR and Src induced by Cx32 overexpression was rescued by cotransfection of siSrc in SMMC-7721 cells. ** , P < 0.01 versus Vector. g and h. Cx32, p-EGFR and Src interacted with each other, as detected by CO-IP experiments in HepG2 cells ( g ) and SMMC-7721 cells ( h ). Lysate supernatants incubated without antibody, termed Input, were used as the positive control, and proteins precipitated by IgG were used as the negative control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1367-13046_2019_1142_fig8_html.pngAnti-Connexin 32/GJB1 Antibody Picoband&reg;Overexpression of Cx32 promotes the proliferation of SMMC-7721 cells and protects cells from SN-induced apoptosis in vivo. a. Representative images of the nude xenograft model. b. Tumor growth curves. Overexpression of Cx32 promoted the tumor growth in nude mice and significantly reduced the growth suppression mediated by intragastric injection of SN (0.5 mg/kg). c. Representative images of tumors from the sacrificed nude mice. d. Representative images of IHC for Cx32, EGFR and Src in tumors generated from SMMC-Vector and SMMC-Cx32 cells. Scale bars: 50 μm. e. Overexpression of Cx32 inhibited the SN-induced increase in the levels of cleaved-caspase3 by increasing the levels of EGFR and Src. ## , P < 0.01 vs Vector +SN. ** , P < 0.01 versus Vector. f. Representative images of IHC for cleaved-caspase3 in each group. Overexpression of Cx32 inhibited the SN-induced increase in the level of cleaved-caspase3. Scale bars: 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1142-y'>30947731</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-connexin-40-gja5-antibody-pa1368-boster.html2026-04-03T05:00:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1368-1-WB-anti-connexin-40-gja5-antibody.jpgAnti-Connexin 40/GJA5 Antibody Picoband&reg;Anti-Connexin 40/GJA5 antibody&#44; PA1368&#44; Western blotting<br>Lane 1: Mouse Heart Tissue Lysate<br>Lane 2: Mouse Heart Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1368-2-IHC-anti-connexin-40-gja5-antibody.jpgAnti-Connexin 40/GJA5 Antibody Picoband&reg;Anti-Connexin 40/GJA5 antibody&#44; PA1368&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsd11b1-antibody-pa1372-1-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1372-1-hsd11b1-primary-antibodies-wb-testing-1.jpgAnti-HSD11B1 Antibody Picoband&reg; Western blot analysis of HSD11B1 using anti-HSD11B1 antibody (PA1372-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br> Lane 3: monkey liver tissue lysates,<br> Lane 4: rat liver tissue lysates,<br> Lane 5: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B1 antigen affinity purified polyclonal antibody (Catalog # PA1372-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B1 at approximately 36 kDa. The expected band size for HSD11B1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1372-1-hsd11b1-primary-antibodies-ihc-testing-2.jpgAnti-HSD11B1 Antibody Picoband&reg; IHC analysis of HSD11B1 using anti-HSD11B1 antibody (PA1372-1). <br> HSD11B1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B1 Antibody (PA1372-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-5ht2a-receptor-antibody-pa1373-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1373-1-WB-anti-5ht2a-receptor-antibody.jpgAnti-5HT2A Receptor/HTR2A Antibody Picoband&reg;Anti-5HT2A Receptor antibody&#44; PA1373&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: Mouse Brain Tissue Lysate<br>Lane 4: Mouse Brain Tissue Lysate<br>Lane 5: U87 Cell Lysate<br>Lane 6: SMMC Cell Lysate<br>Lane 7: HT1080 Cell Lysate<br> Lane 8: COLO320 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1373-2-IHC-anti-5ht2a-receptor-antibody.jpgAnti-5HT2A Receptor/HTR2A Antibody Picoband&reg;Anti-5HT2A Receptor antibody&#44; PA1373&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igf-1-antibody-pa1374-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1374-1-WB-anti-igf-1-antibody.jpgAnti-Insulin-like growth factor I IGF1 Antibody Picoband&reg;Anti-IGF-1 antibody&#44; PA1374&#44; Western blotting<br>WB: Recombinant Human IGF-1 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1374-2-IHC-anti-igf-1-antibody.jpgAnti-Insulin-like growth factor I IGF1 Antibody Picoband&reg;Anti-IGF-1 antibody&#44; PA1374&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1374-41598_2017_article_bfsrep45002_fig3_html.jpgAnti-Insulin-like growth factor I IGF1 Antibody Picoband&reg;Analysis of the target genes of miR-18a in pancreatic progenitor cells. Schematic of the base pairing of miR-18a and its predicted target sequences on the 3′UTR of CTGF, Nedd9, IGF1, and CDK19 ( A ). miR-18a or NC, pMIR-target 3′UTR-luciferase or pMIR-target 3′UTR mut-luciferase, and Renilla pRL-SV40 vectors were co-transfected into 3T3 cells. Forty-eight hours later, Luciferase activity was determined using a Dual-Luciferase Reporter Assay System Kit. All Firefly luciferase values were normalized to the co-transfected Renilla luciferase values ( B ). Pancreatic progenitor cells were transfected with miR-18a or NC. Seventy-two hours after transfection, western blots were performed to determine the expression of CTGF, Nedd9, CDK19, and IGF1. β-actin was used as an endogenous control ( C ). Pancreatic progenitor cells were transfected with miR-18a or NC. Forty-eight hours after transfection, real-time quantitative RT-PCR was performed to determine the expression of CTGF, Nedd9, CDK19 , and IGF1 mRNA ( D ). The data from the miRNA transfected groups were normalized to that of control group. * P < 0.05, ** P < 0.01. Uncropped images for the blots were shown in . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep45002'>28332553</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1374-41598_2017_article_bfsrep45002_fig4_html.jpgAnti-Insulin-like growth factor I IGF1 Antibody Picoband&reg;Effect of miR-18a target gene knock-down on the proliferation of pancreatic progenitor cells. Pancreatic progenitor cells were transfected with siCTGF-1/2, siNedd9-1/2, siCDK19-1/2, and siIGF1-1/2 or sicontrol (NC) for 72 h, and the knockdown of these genes was confirmed by western blot assay ( A ). The proliferation of pancreatic progenitor cells that knocked down the target genes were evaluated by CCK8 assay ( B ) and Ki67 immunofluorescent staining ( C ). The Ki67-positive cells were divided by DAPI-positive cells. The data from the siRNA transfected groups were normalized to that of control group. * P < 0.05, ** P < 0.01. Uncropped images for the blots were shown in . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep45002'>28332553</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek1-antibody-pa1376-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1376-map2k1-primary-antibodies-wb-testing-1.jpgAnti-MEK1/MAP2K1 Antibody Picoband&reg;Western blot analysis of MAP2K1 using anti-MAP2K1 antibody (PA1376). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Daudi whole cell lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K1 antigen affinity purified polyclonal antibody (PA1376) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP2K1 at approximately 45 kDa. The expected band size for MAP2K1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1376-map2k1-primary-antibodies-ihc-testing-1.jpgAnti-MEK1/MAP2K1 Antibody Picoband&reg;IHC analysis of MAP2K1 using anti-MAP2K1 antibody (PA1376). <br>MAP2K1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP2K1 Antibody (PA1376) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1376-map2k1-primary-antibodies-if-testing-1.jpgAnti-MEK1/MAP2K1 Antibody Picoband&reg;IF analysis of MAP2K1 using anti-MAP2K1 antibody (PA1376). <br> MAP2K1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MAP2K1 Antibody (PA1376) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek3-antibody-pa1377-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1377-map2k3-primary-antibodies-wb-testing-1.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg;Western blot analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Caco-2 whole cell lysates,<br> Lane 3: human RT4 whole cell lysates,<br> Lane 4: rat kidney tissue lysates,<br> Lane 5: mouse kidney tissue lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K3 antigen affinity purified polyclonal antibody (PA1377) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP2K3 at approximately 39 kDa. The expected band size for MAP2K3 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1377-map2k3-primary-antibodies-ihc-testing-1.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg;IHC analysis of MAP2K3 using anti-MAP2K3 antibody (PA1377). <br>MAP2K3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP2K3 Antibody (PA1377) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek3-antibody-pa1377-1-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1377-1-1-WB-anti-mek3-antibody.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg;Anti-MEK3 antibody&#44; PA1377-1&#44; All Western blotting<br>All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml<br> Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug<br> Lane 2: Rat Kidney Tissue Lysate at 40ug<br> Lane 3: Rat Spleen Tissue Lysate at 40ug<br> Lane 4: HELA Whole Cell Lysate at 40ug<br> Lane 5: A549 Whole Cell Lysate at 40ug<br> Lane 6: JURKAT Whole Cell Lysate at 40ug<br> Lane 7: CEM Whole Cell Lysate at 40ug<br> Predicted bind size: 39KD<br> Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mdm2-antibody-pa1378-1-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1378-1-1_2.jpgAnti-MDM2 Antibody Picoband&reg; Western blot analysis of MDM2 using anti-MDM2 antibody (PA1378-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: Human Caco-2 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antigen affinity purified polyclonal antibody (Catalog # PA1378-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1378-1-mdm2-primary-antibodies-wb-testing-2.pngAnti-MDM2 Antibody Picoband&reg; Western blot analysis of MDM2 using anti-MDM2 antibody (PA1378-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with non-fat dry milk (5%) in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDM2 antibody (PA1378-1) at 8µL in 6 mLs (not because it needed that concentration but because that was the size of my frozen aliquots) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Cell Signaling Technology's 7074s murine anti-rabbit conformation specific antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an SuperSignal West Pico Plus with a BioRad imager. A specific band was detected for MDM2 at approximately 55 kDa. The expected band size for MDM2 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccl4-mip-1-beta-pa1379-boster.html2026-03-25T05:21:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1379-ccl4-primary-antibodies-wb-testing-1.jpgAnti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband&reg;Western blot analysis of CCL4 using anti-CCL4 antibody (PA1379). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL4 antigen affinity purified polyclonal antibody (PA1379) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCL4 at approximately 15 kDa. The expected band size for CCL4 is at 10 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp10-antibody-pa1380-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1380-1-WB-anti-mmp10-antibody.jpgAnti-Stromelysin-2 MMP10 Antibody Picoband&reg;Anti-MMP10 antibody&#44; PA1380&#44; Western blotting<br>Lane 1: Recombinant Human MMP-10 Protein 10ng<br>Lane 2: Recombinant Human MMP-10 Protein 5ng<br>Lane 3: Recombinant Human MMP-10 Protein 2.5ng<br> Lane 4: HELA Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rank-antibody-pa1382-boster.html2026-03-24T05:03:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1382-1-WB-anti-rank-antibody.jpgAnti-RANK/TNFRSF11A Antibody Picoband&reg;Anti-RANK antibody&#44; PA1382&#44; Western blotting<br>Lane 1: Recombinant Human RANK Protein 10ng<br>Lane 2: Recombinant Human RANK Protein 5ng<br>Lane 3: Recombinant Human RANK Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1382-2-IHC-anti-rank-antibody.jpgAnti-RANK/TNFRSF11A Antibody Picoband&reg;Anti-RANK antibody&#44; PA1382&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp-1-antibody-pa1385-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1385-2-IHC-anti-timp-1-antibody.jpgAnti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband&reg;Anti-TIMP-1 antibody&#44; PA1385&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1385-1_1.jpgAnti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband&reg; Western blot analysis of TIMP1 using anti-TIMP1 antibody (PA1385). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U-87MG whole cell lysates<br> Lane 2: human U2OS whole cell lysates<br> Lane 3: human PC-3 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP1 antigen affinity purified polyclonal antibody (Catalog # PA1385) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP1 at approximately 23KD. The expected band size for TIMP1 is at 23KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1385-metalloproteinase_inhibitor_1_timp1-primary-antibodies-wb-testing-3.jpgAnti-Metalloproteinase inhibitor 1 TIMP1 Antibody Picoband&reg; Western blot analysis of metalloproteinase inhibitor 1 TIMP1 using anti-Metalloproteinase inhibitor 1 TIMP1 antibody (PA1385). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk in TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-Metalloproteinase inhibitor 1 TIMP1 antibody (PA1385) at 1:250 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:2000 for 2 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for metalloproteinase inhibitor 1 TIMP1 at approximately 28 kDa. The expected band size for metalloproteinase inhibitor 1 TIMP1 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsd17b6-antibody-pa1386-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1386-1-WB-anti-hsd17b6-17beta-hsd6-antibody.jpgAnti-HSD17B6 Antibody Picoband&reg;Anti-HSD17B6 antibody&#44; PA1386&#44; Western blotting<br>All lanes: Anti HSD17B6 (PA1386) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 35KD<br>Observed bind size: 35KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1386-2.jpgAnti-HSD17B6 Antibody Picoband&reg; IHC analysis of HSD17B6 using anti-HSD17B6 antibody (PA1386). <br> HSD17B6 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSD17B6 Antibody (PA1386) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apolipoprotein-d-antibody-pa1388-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1388-1_1-WB-anti-apolipoprotein-d-antibody.jpgAnti-Apolipoprotein D/APOD Antibody Picoband&reg;Anti-Apolipoprotein D antibody&#44; PA1388&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: SMMC Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-baff-receptor-antibody-pa1391-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1391-1_1.jpgAnti-BAFF Receptor/TNFRSF13C Antibody Picoband&reg; Western blot analysis of BAFFR using anti-BAFFR antibody (PA1391). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates&#44; <br> Lane 2: human HEK293 whole cell lysates&#44; <br> Lane 3: human K562 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFFR antigen affinity purified polyclonal antibody (Catalog # PA1391) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFFR at approximately 40KD. The expected band size for BAFFR is at 19KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ip-10-cxcl10-antibody-pa1396-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1396-1-WB-anti-cxcl10-ip-10-antibody.jpgAnti-IP10/CXCL10 Antibody Picoband&reg;Anti-IP10 antibody&#44; PA1396&#44; Western blotting<br>Lane 1: Recombinant Human CXCL10 Protein 10ng<br>Lane 2: Recombinant Human CXCL10 Protein 5ng<br>Lane 3: Recombinant Human CXCL10 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1396-2-IHC-anti-cxcl10-ip-10-antibody.jpgAnti-IP10/CXCL10 Antibody Picoband&reg;Anti-IP10 antibody&#44; PA1396&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egf-antibody-pa1398-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1398-1-WB-anti-egf-antibody.jpgAnti-Pro-epidermal growth factor EGF Antibody Picoband&reg;Anti-EGF antibody&#44; PA1398&#44; Western blotting<br>Lane 1: Recombinant Mouse EGF Protein 10ng<br>Lane 2: Recombinant Mouse EGF Protein 5ng<br>Lane 3: Recombinant Mouse EGF Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1398-2-IHC-anti-egf-antibody.jpgAnti-Pro-epidermal growth factor EGF Antibody Picoband&reg;Anti-EGF antibody&#44; PA1398&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegfr1-flt1-antibody-pa1399-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1399-1-WB-anti-vegfr1-flt1-antibody.jpgAnti-VEGF Receptor 1/FLT1 Antibody Picoband&reg;Anti-FLT1 antibody&#44; PA1399&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: SGC Cell Lysate<br>Lane 3: MM231 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fractalkine-cx3cl1-antibody-pa1400-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1400-1-WB-anti-fractalkine-cx3cl1-antibody.jpgAnti-Fractalkine CX3CL1 Antibody Picoband&reg;Anti-CX3CL1 antibody&#44; PA1400&#44; Western blotting<br>Lane 1: Recombinant Human Fractalkine Protein 10ng<br>Lane 2: Recombinant Human Fractalkine Protein 5ng<br>Lane 3: Recombinant Human Fractalkine Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fractalkine-cx3cl1-antibody-pa1401-boster.html2026-03-24T05:03:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1401-1-WB-anti-fractalkine-cx3cl1-antibody.jpgAnti-Fractalkine CX3CL1 Antibody Picoband&reg;Anti-CX3CL1 antibody&#44; PA1401&#44; Western blotting<br>Lane 1: Recombinant Mouse Fractalkin Protein 10ng<br>Lane 2: Recombinant Mouse Fractalkin Protein 5ng<br>Lane 3: Recombinant Mouse Fractalkin Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1401-2-IHC-anti-fractalkine-cx3cl1-antibody.jpgAnti-Fractalkine CX3CL1 Antibody Picoband&reg;Anti-CX3CL1 antibody&#44; PA1401&#44; IHC(P)<br>IHC(P): Rat Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gad65-antibody-pa1403-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1403-gad2-primary-antibodies-wb-testing-1.jpgAnti-GAD65/GAD2 Antibody Picoband&reg; Western blot analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (PA1403). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD65/GAD2 antigen affinity purified polyclonal antibody (Catalog # PA1403) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD65/GAD2 at approximately 65 kDa. The expected band size for GAD65/GAD2 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1403-gad2-primary-antibodies-ihc-testing-2.jpgAnti-GAD65/GAD2 Antibody Picoband&reg; IHC analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (PA1403). <br> GAD65/GAD2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAD65/GAD2 Antibody (PA1403) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mapk8-9-antibody-pa1407-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1407-1-WB-anti-mapk8-9-antibody.jpgAnti-MAPK8/9 Antibody Picoband&reg;Anti-MAPK8/9 antibody&#44; PA1407&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Thymus Tissue Lysate<br>Lane 3: MCF-7 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: JURKAT Cell Lysate<br>Lane 6: MM231 Cell Lysate<br>Lane 7: CEM Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1407-2-IHC-anti-mapk8-9-antibody.jpgAnti-MAPK8/9 Antibody Picoband&reg;Anti-MAPK8/9 antibody&#44; PA1407&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1407-3-IHC-anti-mapk8-9-antibody.jpgAnti-MAPK8/9 Antibody Picoband&reg;Anti-MAPK8/9 antibody&#44; PA1407&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pi-3-kinase-p85-alpha-antibody-pa1410-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1410-1-WB-anti-pi-3-kinase-p85-alpha-antibody.jpgAnti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband&reg;Anti-PI 3 Kinase p85 alpha antibody&#44; PA1410&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: COLO-320 whole cell lysate<br>Lane 4: SW620 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1410-2.jpgAnti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband&reg; Western blot analysis of PI 3 Kinase p85 alpha using anti-PI 3 Kinase p85 alpha antibody (PA1410). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates<br> Lane 2: rat lung tissue lysates<br> Lane 3: rat PC-12 whole cell lysates<br> Lane 4: mouse brain tissue lysates<br> Lane 5: mouse HEPA1-6 whole cell lysates<br> Lane 6: mouse RAW246.7 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI 3 Kinase p85 alpha antigen affinity purified polyclonal antibody (Catalog # PA1410) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PI 3 Kinase p85 alpha at approximately 85KD. The expected band size for PI 3 Kinase p85 alpha is at 85KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-podoplanin-gp36-antibody-pa1411-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1411-1_1-WB-anti-podoplanin-gp36-antibody.jpgAnti-Podoplanin/gp36/PDPN Antibody Picoband&reg;Anti-Podoplanin/gp36 antibody&#44; PA1411&#44; Western blotting<br>All lanes: Anti Podoplanin/gp36 (PA1411) at 0.5ug/ml<br>Lane 1: SMMC Whole Cell Lysate at 40ug<br>Lane 2: 293T Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 36KD<br>Observed bind size: 36KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-progesterone-receptor-antibody-pa1413-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1413-1-WB-anti-progesterone-receptor-antibody.jpgAnti-Progesterone Receptor/PGR Antibody Picoband&reg;Anti-Progesterone Receptor antibody&#44; PA1413&#44; Western blotting<br>WB: HELA Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1413-2-IHC-anti-progesterone-receptor-antibody.jpgAnti-Progesterone Receptor/PGR Antibody Picoband&reg;Anti-Progesterone Receptor antibody&#44; PA1413&#44; IHC(P)<br>IHC(P): Human Rectal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pleiotrophin-antibody-pa1414-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1414-ptn-primary-antibodies-ihc-testing-3.jpgAnti-Pleiotrophin/PTN Antibody Picoband&reg;IHC analysis of PTN using anti-PTN antibody (PA1414). <br>PTN was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTN Antibody (PA1414) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1414-ptn-primary-antibodies-wb-testing-1.jpgAnti-Pleiotrophin/PTN Antibody Picoband&reg; Western blot analysis of PTN using anti-PTN antibody (PA1414). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brian tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTN antigen affinity purified polyclonal antibody (PA1414) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTN at approximately 19 kDa. The expected band size for PTN is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1414-ptn-primary-antibodies-ihc-testing-2.jpgAnti-Pleiotrophin/PTN Antibody Picoband&reg; IHC analysis of PTN using anti-PTN antibody (PA1414). <br> PTN was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PTN Antibody (PA1414) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ndrg1-antibody-pa1416-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1416-ndrg1-primary-antibodies-wb-testing-1.jpgAnti-Protein NDRG1 NDRG1 Antibody Picoband&reg;Western blot analysis of NDRG1 using anti-NDRG1 antibody (PA1416). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG1 antigen affinity purified polyclonal antibody (PA1416) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDRG1 at approximately 43 kDa. The expected band size for NDRG1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1416-ndrg1-primary-antibodies-if-testing-1.jpgAnti-Protein NDRG1 NDRG1 Antibody Picoband&reg;IF analysis of NDRG1 using anti-NDRG1 antibody (PA1416). <br> NDRG1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDRG1 Antibody (PA1416) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thrombospondin-2-antibody-pa1417-boster.html2026-03-24T05:03:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-ajcr0014-3433-f1.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg;THBS2 expression is upregulated in GC and predicts poor survival in GC patients. A. A comparison between three datasets with 1213, 1738, and 1305 DEGs, revealed a total of 137 common DEGs between GC and normal tissues. B. Network of the top 10 genes, with a higher ranking indicated by a more intense red. C. Genetic mutation analysis of 10 hub genes in GC. D. Expression of THBS2 mRNA in different tumor types including gastric cancer (GC) and normal adjacent tissue, according to TCGA database analysis. E. Relationship between THBS2 and T stage of tumor. F. Expression of THBS2 mRNA in GC according to GEO databases analysis. G, H. Expression of THBS2 mRNA and protein in gastric cancer cell line and GES-1. I. The relative expression level of THBS2 mRNA in 30 pairs of GC and normal adjacent tissues. J, K. THBS2 protein expression and IHC analysis in GC and adjacent normal tissues. L. The prognostic survival of THBS2 was analyzed based on K-M plotter (OS, n=875; FPS, n=640; PPS, n=498). Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ns = no significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11301304/'>39113869</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-ajcr0014-3433-f2.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg;THBS2 regulates the progression and stemness of GC cells in vitro. A, B. WB and qRT-PCR validate the efficiency of constructed THBS2 knockdown and overexpression gastric cancer cell lines. C, D. The migration and invasion ability of sh-THBS2 and OE-THSB2 groups were detected by Transwell assay. E, F. Wound healing assay was used to detect the migration distance of sh-THBS2 and OE-THSB2 groups. G, H. The proliferation ability of sh-THBS2 and OE-THSB2 groups was detected by CCK8 assay. I, J. The proliferative potential of sh-THBS2 and OE-THSB2 groups was verified by colony formation experiment. K, L. Apoptosis assay showed the proportions of apoptotic HGC-27 and AGS cells in sh-THBS2 and OE-THSB2 groups. M, N. The cell cycle of sh-THBS2 and OE-THSB2 groups was detected by flow cytometry. O. The Sphere-forming abilities was detected in HGC-27 and AGS cells after THBS2 intervention. P. The expression of CD44 in HGC-27 and AGS cells after THBS2 intervention was detected by flow cytometry. Q. Expression of proliferation, apoptosis, invasion and EMT-related proteins in HGC-27 and AGS cells after THBS2 intervention. Data represent the mean ± SD. **P < 0.01, ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11301304/'>39113869</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-ajcr0014-3433-f4.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg;THBS2 reversed Mir-29b-3p-mediated inhibition of GC. A, B. The protein expression of THBS2 in HGC-27 and AGS that co-transfected miR-29b-3p mimic or inhibitor and THBS2 knockdown or overexpression plasmid. C, D. The proliferation of HGC-27 and AGS cells that co-transfected miR-29b-3p mimic or inhibitor and THBS2 knockdown or overexpression plasmid was determined by CCK-8 assay. E, F. The proliferation of HGC-27 and AGS cells that co-transfected miR-29b-3p mimic or inhibitor and THBS2 knockdown or overexpression plasmid was determined by colony formation experiment. G, H. The migration and invasion ability of HGC-27 and AGS cells that co-transfected miR-29b-3p mimic or inhibitor and THBS2 knockdown or overexpression plasmid was determined by transwell assay. I, J. The migration distance of HGC-27 and AGS cells that co-transfected miR-29b-3p mimic or inhibitor and THBS2 knockdown or overexpression plasmid was determined by wound healing assay. Data represent the mean ± SD. **P < 0.01, ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11301304/'>39113869</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-ajcr0014-3433-f3.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg;MiR-29b-3p directly targeted the 3’-UTR of THBS2 mRNA and down-regulated THBS2 in GC. A. Bioinformatics predicts THBS2-targeting miRNAs. B. THBS2 mRNA expression of 4 candidate miRNAs transfected into gastric cancer cells. C. The heatmap was drawn to exhibit the decrease extent of THBS2 mRNA. D. The protein expression of THBS2 GC cells after transfection with different kinds of miRNA mimics. E, F. Relative expression of miR-29b-3p in GC and normal tissues in TCGA. G. Expression of THBS2 protein after miR-29b-3p transfected into GC cell lines and GES-1. H. The relative expression level of miR-29b-3p in 30 pairs of GC and normal adjacent tissues. I, J. Correlation between miR-29b-3p andTHBS2 mRNA expression in GC tissues in TCGA and 30 pairs clinic tissues. K, L. The mRNA Expression of THBS2 in HGC-27 and AGS after transfection with miR-29b-3p inhibitor and mimics. Data represent the mean ± SD. **P < 0.01. M. The potential miR-29b-3p binding sites in 3’-UTR of THBS2 mRNA predicted by TargetScan and the sequences of the WT-THBS2 and MUT-THBS2 3’-UTR reporter plasmids. N. Dual luciferase reporter assay validated of miR-29b-3p targeting THBS2. O, P. Colony formation experiment for AGS and HGC-27 cells with miR-29b-3p mimic or inhibitor. Q, R. CCK8 assay for AGS and HGC-27 cells with miR-29b-3p mimic or inhibitor. S, T. Wound healing assay for AGS and HGC-27 cells with miR-29b-3p mimic or inhibitor. U, V. Transwell assay for AGS and HGC-27 cells with miR-29b-3p mimic or inhibitor. Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11301304/'>39113869</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-ajcr0014-3433-f5.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg;THBS2 affects the progression and stemness of GC through Notch signaling pathway. A. GSEA showed the relationship between the expression of THBS2 and Notch signaling pathway. B. Correlations among THBS2 and Notch1, Notch2, Notch3, and Notch4 analyzed on the basis of TCGA database. C. The protein level of Notch3, Notch target genes or EMT markers (such as NICD, Hey1, Hes1, E-cadherin, N-cadherin, and Vimentin) was assessed in sh-THBS2 and OE-THBS2 groups vs. control by Western Blotting. D. The Sphere-forming abilities was detected in Vector, THBS2 or THBS2 + DAPT groups. Data represent the mean ± SD. ***P < 0.001. E. The Sphere-forming abilities was detected in knockdown THBS2 HGC-27 cells with or without overexpressing Notch3. Data represent the mean ± SD. **P < 0.01. F. The expression levels of Notch3, Hey1 and Hes1 proteins in AGS cell with Vector, OE-THBS2 or OE-THBS2 + DAPT groups were detected by WB. G. The Notch3, Nanog, Sox2, and OCT4 protein expression levels in knockdown THBS2 HGC-27 cells with or without overexpressing Notch3 were analyzed by WB.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11301304/'>39113869</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-ajcr0014-3433-f6.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg;Down-regulation of THBS2 inhibits GC tumorigenesis in vivo. A. Images of tumors from nude mice in the sh-Ctrl and sh-THBS2 groups. B, C. Tumor weights and volumes in the two groups. Data represent the mean ± SD. **P < 0.01, ***P < 0.001. D. Images of liver metastatic tumors after injection of HGC-27 cells (sh-Ctrl and sh-THBS2 groups) into the spleen. E. H&E staining about liver metastasis of spleen injected mice, 2× and 20×. F. The mRNA level of THBS2, EMT markers and Notch3 in established xenograft model assessed by qRT-PCR. Data represent the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. G. The protein level of THBS2, EMT markers and Notch3 in established xenograft model assessed by WB. H. Overall schematic presentation of miR-29b-3p/THBS2/NOTCH3 cascade in gastric carcinogenesis.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11301304/'>39113869</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-gr4.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg;GSEA of hub genes. (A).Enrichment plot of THBS2 from GSEA in IPF group. (B).Enrichment plot of THBS2 from GSEA in RA-UIP group. (C).Enrichment plot of TIMP1 from GSEA in IPF group. (D).Enrichment plot of TIMP1 from GSEA in RA-UIP group. (E).Enrichment plot of POSTN from GSEA in IPF group. (F).Enrichment plot of POSTN from GSEA in RA-UIP group. (G). Enrichment plot of CD19 from GSEA in IPF group. (H).Enrichment plot of CD19 from GSEA in RA-UIP group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)04119-7'>38571583</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1417-thbs2-primary-antibodies-wb-testing-1.jpgAnti-Thrombospondin 2/THBS2 Antibody Picoband&reg; Western blot analysis of THBS2 using anti-THBS2 antibody (PA1417). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human U87 whole cell lysates,<br> Lane 4: human U251 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THBS2 antigen affinity purified polyclonal antibody (Catalog # PA1417) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THBS2 at approximately 160 kDa. The expected band size for THBS2 is at 130 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ubiquitin-antibody-pa1420-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1420-1-WB-anti-ubiquitin-antibody.jpgAnti-Ubiquitin/UBB Antibody Picoband&reg;Anti-Ubiquitin antibody&#44; PA1420&#44; Western blotting<br>Lane 1: Rat Thymus Tissue Lysate<br>Lane 2: Human MCF-7 Cell Lysate<br>Lane 3: MM231 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: SMMC Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1420-2-IHC-anti-ubiquitin-antibody.jpgAnti-Ubiquitin/UBB Antibody Picoband&reg;Anti-Ubiquitin antibody&#44; PA1420&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fetuin-a-antibody-pa1421-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1421-ahsg-primary-antibodies-wb-testing-1.jpgAnti-Alpha-2-HS-glycoprotein AHSG Antibody Picoband&reg; Western blot analysis of Fetuin A using anti-Fetuin A antibody (PA1421). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HCCT tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fetuin A antigen affinity purified polyclonal antibody (Catalog # PA1421) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fetuin A at approximately 55-60 kDa. The expected band size for Fetuin A is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-galectin-1-antibody-pa1422-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-wb-testing-1_1.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; Western blot analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human A375 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat kidney tissue lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGALS1 antigen affinity purified polyclonal antibody (Catalog # PA1422) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LGALS1 at approximately 15 kDa. The expected band size for LGALS1 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-ihc-testing-2.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> LGALS1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LGALS1 Antibody (PA1422) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-ihc-testing-3.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> LGALS1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LGALS1 Antibody (PA1422) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-ihc-testing-4.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> LGALS1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LGALS1 Antibody (PA1422) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-ihc-testing-5.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> LGALS1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LGALS1 Antibody (PA1422) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-ihc-testing-6.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> LGALS1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LGALS1 Antibody (PA1422) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-ihc-testing-7.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> LGALS1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LGALS1 Antibody (PA1422) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-if-testing-8.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IF analysis of LGALS1 using anti-LGALS1 antibody (PA1422). <br> LGALS1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LGALS1 Antibody (PA1422) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1422-lgals1-primary-antibodies-fcm-testing-9.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-LGALS1 antibody (PA1422).<br>Overlay histogram showing PC-3 cells stained with PA1422 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LGALS1 Antibody (PA1422,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-3-antibody-pa1423-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1423-1-WB-anti-il-3-antibody.jpgAnti-Interleukin-3 IL3 Antibody Picoband&reg;Anti-IL-3 antibody&#44; PA1423&#44; Western blotting<br>Lane 1: Recombinant Human IL-3 Protein 10ng<br>Lane 2: Recombinant Human IL-3 Protein 5ng<br>Lane 3: Recombinant Human IL-3 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-4-antibody-pa1424-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1424-1-WB-anti-il-4-antibody.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Anti-IL-4 antibody&#44; PA1424&#44; Western blotting<br>Lane 1: Recombinant Human IL-4 Protein 10ng<br>Lane 2: Recombinant Human IL-4 Protein 5ng<br>Lane 3: Recombinant Human IL-4 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-carbonic-anhydrase-i-antibody-pa1425-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1425-ca1-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; Western blot analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (PA1425). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates, <br> Lane 2: rat spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase I/CA1 antigen affinity purified polyclonal antibody (Catalog # PA1425) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 29 kDa. The expected band size for Carbonic Anhydrase I/CA1 is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcl2l2-antibody-pa1426-1-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1426-1-1_2.jpgAnti-Bcl-2-like protein 2 Bcl2L2 Antibody Picoband&reg; Western blot analysis of Bcl2L2 using anti-Bcl2L2 antibody (PA1426-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates&#44;<br> Lane 2: human K562 whole cell lysates&#44;<br> Lane 3: human U-937 whole cell lysates&#44; <br> Lane 4: human Hela whole cell lysates.&#44;<br> Lane 5: human A431 whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl2L2 antigen affinity purified polyclonal antibody (Catalog # PA1426-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl2L2 at approximately 21KD. The expected band size for Bcl2L2 is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1426-1-2-IHC-anti-bcl2l2-bcl-w-antibody.jpgAnti-Bcl-2-like protein 2 Bcl2L2 Antibody Picoband&reg; IHC analysis of BCL2L2 using anti-BCL2L2 antibody (PA1426-1).<br> BCL2L2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BCL2L2 Antibody (PA1426-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1426-1-bcl2l2-primary-antibodies-fc-testing-3.jpgAnti-Bcl-2-like protein 2 Bcl2L2 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-BCL2L2 antibody (PA1426-1). <br>Overlay histogram showing PC-3 cells stained with PA1426-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCL2L2 Antibody (PA1426-1&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk4-antibody-pa1428-1-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1428-1-cdk4-primary-antibodies-wb-testing-1.jpgAnti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband&reg; Western blot analysis of CDK4 using anti-CDK4 antibody (PA1428-1, Left) and anti-CDK4 antibody (PB9535, Right). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: rat PC-12 whole cell lysates, <br> Lane 4: mosue NIH/3T3 whole cell lysates, <br> Lane 5: mosue RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PA1428-1) and rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PB9535)at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1428-1-cdk4-primary-antibodies-if-testing-2.jpgAnti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband&reg; IF analysis of CDK4 using anti-CDK4 antibody (PA1428-1) and anti-Tubulin Alpha antibody (M03989-3).<br>CDK4 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CDK4 Antibody (PA1428-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1428-1-cdk4-primary-antibodies-fcm-testing-3.jpgAnti-Cyclin-dependent kinase 4 Cdk4 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-CDK4 antibody (PA1428-1). <br>Overlay histogram showing MCF-7 cells stained with PA1428-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK4 Antibody (PA1428-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. <a href="https://www.bosterbio.com/blog/post/antibody-isotypes-igg-iga-igm-ige-igd">Isotype control antibody</a> (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcl9-antibody-pa1429-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1429-1-WB-anti-cxcl9-mig-antibody.jpgAnti-MIG/CXCL9 Antibody Picoband&reg;Anti-CXCL9 antibody&#44; PA1429&#44; Western blotting<br>Lane 1: Recombinant Human CXCL9 Protein 10ng<br>Lane 2: Recombinant Human CXCL9 Protein 5ng<br>Lane 3: Recombinant Human CXCL9 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1429-2-IHC-anti-cxcl9-mig-antibody.jpgAnti-MIG/CXCL9 Antibody Picoband&reg;Anti-CXCL9 antibody&#44; PA1429&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-osteopontin-antibody-pa1431-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1431-spp1-primary-antibodies-wb-testing-1.jpgAnti-Osteopontin/SPP1 Antibody Picoband&reg; Western blot analysis of OPN/SPP1 using anti-OPN/SPP1 antibody (PA1431). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPN/SPP1 antigen affinity purified polyclonal antibody (Catalog # PA1431) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPN/SPP1 at approximately 65 kDa. The expected band size for OPN/SPP1 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angiopoietin-like-4-pa1435-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1435-angptl4-primary-antibodies-wb-testing-1.jpgAnti-Angiopoietin-like 4/ANGPTL4 Antibody Picoband&reg; Western blot analysis of ANGPTL4 using anti-ANGPTL4 antibody (PA1435). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human U87 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPTL4 antigen affinity purified polyclonal antibody (Catalog # PA1435) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPTL4 at approximately 45 kDa. The expected band size for ANGPTL4 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-vi-antibody-pa1436-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1436-1-WB-anti-annexin-vi-antibody.jpgAnti-Annexin VI/ANXA6 Antibody Picoband&reg;Anti-Annexin VI antibody&#44; PA1436&#44; Western blotting<br>Lane 1: Rat Ovary Tissue Lysate<br>Lane 2: Rat Liver Tissue Lysate<br>Lane 3: Rat Intestinum Tenue Tissue Lysate<br>Lane 4: Rat Brain Tissue Lysate<br>Lane 5: A549 Cell Lysate<br>Lane 6: JURKAT Cell Lysate<br> Lane 7: RAJI Cell Lysate<br> Lane 8: CEM Cell Lysate<br> Lane 9: SMMC Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1436-2-IHC-anti-annexin-vi-antibody.jpgAnti-Annexin VI/ANXA6 Antibody Picoband&reg;Anti-Annexin VI antibody&#44; PA1436&#44; IHC(P)<br>IHC(P): Rat Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1436-3-IHC-anti-annexin-vi-antibody.jpgAnti-Annexin VI/ANXA6 Antibody Picoband&reg;Anti-Annexin VI antibody&#44; PA1436&#44; IHC(F)<br>IHC(F): Rat Spleen Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bak-antibody-pa1437-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1437-bak1-primary-antibodies-wb-testing-1.jpgAnti-BAK/BAK1 Antibody Picoband&reg; Western blot analysis of BAK/BAK1 using anti-BAK/BAK1 antibody (PA1437). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human CACO-2 whole cell lysates, <br> Lane 5: human U20S whole cell lysates, <br> Lane 6: human HEPG2 whole cell lysates, <br> Lane 7: human HELA whole cell lysates, <br> Lane 8: human A549 whole cell lysates, <br> Lane 9: rat heart tissue lysates, <br> Lane 10: rat lung tissue lysates, <br> Lane 11: mouse heart tissue lysates, <br> Lane 12: mouse lung tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK/BAK1 antigen affinity purified polyclonal antibody (Catalog # PA1437) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAK/BAK1 at approximately 23KD. The expected band size for BAK/BAK1 is at 23KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1437-bak1-primary-antibodies-ihc-testing-2.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK/BAK1 using anti-BAK/BAK1 antibody (PA1437). <br> BAK/BAK1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BAK/BAK1 Antibody (PA1437) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1437-bak1-primary-antibodies-ihc-testing-3.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK/BAK1 using anti-BAK/BAK1 antibody (PA1437). <br> BAK/BAK1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-BAK/BAK1 Antibody (PA1437) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1437-bak1-primary-antibodies-if-testing-4.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IF analysis of BAK/BAK1 using anti-BAK/BAK1 antibody (PA1437). <br> BAK/BAK1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-BAK/BAK1 Antibody (PA1437) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ca3-antibody-pa1439-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1439-ca3-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase III/CA3 Antibody Picoband&reg;Western blot analysis of CA3 using anti-CA3 antibody (PA1439). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat fat tissue lysates,<br> Lane 4: mouse liver tissue lysates,<br> Lane 5: mouse fat tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA3 antigen affinity purified polyclonal antibody (PA1439) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CA3 at approximately 30 kDa. The expected band size for CA3 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1439-2-IHC-anti-ca3-carbonic-anhydrase-iii-antibody.jpgAnti-Carbonic Anhydrase III/CA3 Antibody Picoband&reg;Anti-CA3 antibody&#44; PA1439&#44; IHC(P)<br>IHC(P): Rat Skeletal Muscle Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-1-p20-antibody-pa1440-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1440-2-IHC-anti-caspase-1-p20-antibody.jpgAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg;Anti-Caspase-1(P20) antibody&#44; PA1440&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1440-1_1-WB-anti-caspase-1-p20-antibody.jpgAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg;Anti-Caspase-1(P20) antibody&#44; PA1440&#44; Western blotting<br>Lane 1: JURKAT Cell Lysate <br>Lane 2: RAJI Cell Lysate<br>Lane 3: CEM Cell Lysate <br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-1-p20-antibody-pa1440-1-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1440-1-12931_2025_3222_fig2_html.pngAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg;Impact of PITX1 on pyroptosis in hypoxia-induced PASMCs. A GSEA: The functions of PITX1 were enriched mainly in the inflammasome signaling pathway under hypoxic conditions. B , C Western blot: The interference efficiency of siPITX1 and the overexpression efficiency of the PITX1 plasmid. D , I Western blot: Representative images and statistical data of CASP1, GSDMD-N, IL-1β, and IL-18 levels. E , J YPI/PI staining: YPI is a green fluorescent dye permeable to the membrane of apoptotic cells, and PI staining of necrotic cells with compromised membrane integrity may result in the emission of red fluorescence. Scale bar: 100 μm. F , K DiO staining: Green fluorescence staining of the cell membrane; and the membranes of live cells exhibit green fluorescence. Scale bar: 50 μm. G , H LDH assay: Determination of the number of dead cells by measuring the amount of LDH released from the membrane of damaged cells. Nor normoxic, Hyp hypoxic, NC noncoding nucleotides, siPITX PITX1 siRNA, and p-PITX PITX1 plasmid. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1440-1-12931_2025_3222_fig3_html.pngAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg;Reversal of pulmonary vascular remodeling and pyroptosis in the hypoxic PH and SuHx PAH mouse models by overexpressing PITX1. A Construction of the PITX1 AAV5 plasmid and establishment of the SuHx PAH model under normoxic and hypoxic (10% O 2 ) conditions with the overexpression of PITX1 via AAV5 in mice. B , I Western blot: Representative images of the overexpression efficiency of PITX1 plasmid in the SuHx PAH and hypoxic groups. C , J Immunofluorescence staining: Overexpression of PITX1 in the SuHx PAH and hypoxic groups as well as statistical analysis. Scale bar: 50 μm. D , K Representative echocardiograms (left) and statistical analysis of PAAT (right 1), and PAVTI (right 2), as measured by echocardiography. E , L Right heart catheterization and the right ventricular hypertrophy index. F , G , M , N Representative images of H&E and Masson’s trichrome staining in the lung tissues of the modeled mice. Scale bar: 50 μm. H , O Western blot: Representative images as well as statistical data of NLRP3, ASC, CASP1, GSDMD-N and IL18 levels. PAAT pulmonary artery acceleration time, PAVTI pulmonary artery velocity time integral, AAV AAV5, adeno-associated virus 5. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1440-1-12931_2025_3222_fig6_html.pngAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg;Modulation of pyroptosis by influencing DUSP4 through its SE. A , B Construction of the dual-luciferase plasmid as well as statistical analysis of luciferase activity in mPASMCs cotransfected with PITX1 plasmid and three segments of SE-DUSP4 or corresponding mutants for 24 h. C , E RT‒qPCR: Changes in the transcript level of DUSP4 in mPASMCs at the 24-h time point under hypoxic conditions after transfection with PITX1 plasmid and three segments of SE-DUSP4 after the addition of inhibitors JQ1 and iBET. D , F Western blot: Representative images as well as statistical data of the protein expression of DUSP4 in mPASMCs at the 24-h time point under hypoxic conditions after transfection with PITX1 plasmid and three segments of SE-DUSP4 after the addition of inhibitors JQ1 and iBET. G Representative images and statistical data of positive CASP1 staining. Scale bar: 50 μm. H Western blot: Representative images as well as statistical analysis of the protein levels of GSDMD-N, IL-1β and IL-18. Nor normoxic, Hyp hypoxic. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1440-1-12931_2025_3222_fig7_html.pngAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg;PITX1 modulating pulmonary vascular remodeling and pyroptosis through DUSP4. A Establishment of a hypoxic (10% O 2 ) mouse model with overexpression of PITX1 and knockdown of DUSP4 via AAV5. B Representative echocardiogram (left) as well as statistical analysis of PAAT (right 1) and PAVTI (right 2). C Statistical analysis of right ventricular pressure and right ventricular hypertrophy index. D , E Representative images of H&E and Masson’s trichrome staining in the lung tissue of the modeled mice. Scale bar: 50 μm. F Western blot: Representative images as well as statistical analysis of NLRP3, ASC, CASP1, GSDMD-N and IL-1β levels. G , H Immunofluorescence staining of PITX1 (red) with an antibody and DUSP4 (green) with fluorescence in situ hybridization (FISH) probes to demonstrate their colocalization in the lung tissues and mPASMCs of mice. Nuclei were counterstained with DAPI (blue). Scale bars: 25 μm, and 100 μm. I Western blot: Representative image showing the interference efficiency of siDUSP4. J LDH assay: Determination of the number of dead mPASMCs by quantifying the amount of LDH released from the membrane of damaged cells. K SEM: Morphological observation of nuclear, cytoplasmic, and membrane integrity as well as pore formation following PITX1 overexpression combined with DUSP4 knockdown, compared with those under hypoxic conditions and with PITX1 overexpression alone. Scale bar: 5 μm. L Western blot: Representative images as well as statistical data of CASP1, GSDMD-N, IL-1β, and IL-18 levels. PAAT pulmonary artery acceleration time, PAVTI pulmonary artery velocity time integral, Nor normoxic, Hyp hypoxic, NC noncoding nucleotides, shDUSP4 shRNADUSP4, AAV AAV5, adeno-associated virus 5. All values are presented as means ± SEMs (* p < 0.05, and ** p < 0.01; n ≥ 3) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-025-03222-9'>40241046</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1440-1-12974_2020_1718_fig5_html.pngAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg;The application of SR9009 for 7 days reduced the expression of NLRP3 inflammasome-related proteins and inflammatory cytokines in the hippocampus after SE. a The expression of NLRP3, ASC, Caspase1, IL-1β, IL-18, IL-6, and TNF-α was detected by western blotting assay. b Densitometric analysis results of NLRP3, ASC, Caspase1, IL-1β, IL-18, IL-6, and TNF-α in the four groups. n = 5 for each group. The data presented as the mean ± SD. NS, not significant, * p < 0.05, † p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-1718-7'>32005256</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1440-1-casp1-primary-antibodies-wb-testing-1.jpgAnti-Caspase-1(P20)/CASP1 Antibody Picoband&reg; Western blot analysis of CASP1 using anti-CASP1 antibody (PA1440-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: rat C6 whole cell lysates,<br> Lane 3: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 antigen affinity purified polyclonal antibody (Catalog # PA1440-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP1 at approximately 46 kDa. The expected band size for CASP1 is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-6-p18-antibody-pa1441-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1441-casp6-primary-antibodies-wb-testing-1.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg; Western blot analysis of Caspase-6(P18) using anti-Caspase-6(P18) antibody (PA1441). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-6(P18) antigen affinity purified polyclonal antibody (Catalog # PA1441) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-6(P18) at approximately 35 kDa. The expected band size for Caspase-6(P18) is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1441-casp6-primary-antibodies-ihc-testing-2.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg; IHC analysis of Caspase-6(P18) using anti-Caspase-6(P18) antibody (PA1441). <br> Caspase-6(P18) was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase-6(P18) Antibody (PA1441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1441-casp6-primary-antibodies-fcm-testing-4.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-Caspase-6(P18) antibody (PA1441). <br>Overlay histogram showing SiHa cells stained with PA1441 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caspase-6(P18) Antibody (PA1441, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1441-casp6-primary-antibodies-ihc-testing-3.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg; IHC analysis of Caspase-6(P18) using anti-Caspase-6(P18) antibody (PA1441). <br> Caspase-6(P18) was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase-6(P18) Antibody (PA1441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-6-p18-antibody-pa1441-1-boster.html2026-03-24T05:03:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1441-1-2-IHC-anti-caspase-6-p18-antibody.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg;Anti-Caspase-6(P18) antibody&#44; PA1441-1&#44; IHC(P)<br>IHC(P): Rat Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1441-1-3-IHC-anti-caspase-6-p18-antibody.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg;Anti-Caspase-6(P18) antibody&#44; PA1441-1&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1441-1-4-IHC-anti-caspase-6-p18-antibody.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg;Anti-Caspase-6(P18) antibody&#44; PA1441-1&#44; IHC(P)<br>IHC(P): Mouse Lung Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1441-1-5-IHC-anti-caspase-6-p18-antibody.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg;Anti-Caspase-6(P18) antibody&#44; PA1441-1&#44; IHC(F)<br>IHC(F): Rat Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1441-1-1_1.jpgAnti-Caspase-6(P18)/CASP6 Antibody Picoband&reg;Anti-Caspase-6(P18) antibody&#44; PA1441-1&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Rat Kidney Tissue Lysate<br>Lane 3: Rat Testis Tissue Lysate<br>Lane 4: NRK Cell Lysate<br>Lane 5: Mouse Liver Tissue Lysate<br>Lane 6: Mouse Kidney Tissue Lysate<br>Lane 7: Mouse Testis Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-7-p11-antibody-pa1442-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1442-1_1-WB-anti-caspase-7-p11-antibody.jpgAnti-Caspase-7(P11)/CASP7 Antibody Picoband&reg;Anti-Caspase-7(P11) antibody&#44; PA1442&#44; Western blotting<br>All lanes: Anti CASP7(p11) (PA1442) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Lane 3: Rat Liver Tissue Lysate at 50ug<br>Lane 4: Rat Kidney Tissue Lysate at 50ug<br>Predicted bind size: 34KD<br>Observed bind size: 34KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1442-2-IHC-anti-caspase-7-p11-antibody.jpgAnti-Caspase-7(P11)/CASP7 Antibody Picoband&reg;Anti-Caspase-7(P11) antibody&#44; PA1442&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd14-antibody-pa1443-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1443-cd14-primary-antibodies-wb-testing-1.jpgAnti-CD14 Antibody Picoband&reg; Western blot analysis of CD14 using anti-CD14 antibody (PA1443). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 antigen affinity purified polyclonal antibody (Catalog # PA1443) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD14 at approximately 50 kDa. The expected band size for CD14 is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pf4-antibody-pa1446-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1446-cxcl4-primary-antibodies-wb-testing-1.jpgAnti-Platelet factor 4 PF4 Antibody Picoband&reg;Western blot analysis of CXCL4/PF4 using anti-CXCL4/PF4 antibody (PA1446). <br>Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human CXCL4 protein 5ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL4/PF4 antigen affinity purified polyclonal antibody (PA1446) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CXCL4/PF4 at approximately 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1446-cxcl4-primary-antibodies-ihc-testing-1.jpgAnti-Platelet factor 4 PF4 Antibody Picoband&reg;IHC analysis of CXCL4/PF4 using anti-CXCL4/PF4 antibody (PA1446). <br>CXCL4/PF4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL4/PF4 Antibody (PA1446) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddt-antibody-pa1449-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1449-ddt-primary-antibodies-wb-testing-1.jpgAnti-D-dopachrome decarboxylase DDT Antibody Picoband&reg; Western blot analysis of DDT using anti-DDT antibody (RP1011, Left) and anti-DDT antibody (PA1449, Right). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, <br> Lane 2: rat liver tissue lysates,<br> Lane 3: mouse liver tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # RP1011) and rabbit anti-DDT antigen affinity purified polyclonal antibody (Catalog # PA1449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDT at approximately 13-15 kDa. The expected band size for DDT is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1449-2-IHC-anti-ddt-antibody.jpgAnti-D-dopachrome decarboxylase DDT Antibody Picoband&reg; IHC analysis of DDT using anti-DDT antibody (PA1449). <br> DDT was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDT Antibody (PA1449) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-defensin-1-antibody-pa1450-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1450-1-IHC-anti-beta-defensin-1-antibody.jpgAnti-beta Defensin 1/DEFB1 Antibody Picoband&reg;Anti-beta Defensin 1 antibody&#44; PA1450&#44; IHC(P)<br>IHC(P): Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1450-2-WB-anti-beta-defensin-1-antibody.jpgAnti-beta Defensin 1/DEFB1 Antibody Picoband&reg;Anti-beta Defensin 1 antibody&#44; PA1450&#44;Western blotting<br>All lanes: Anti Defensin 1 (PA1450) at 0.5ug/ml<br>WB : COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 7KD<br>Observed bind size: 7KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eotaxin-3-antibody-pa1451-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1451-1-WB-anti-eotaxin-3-antibody.jpgAnti-Eotaxin 3/CCL26 Antibody Picoband&reg;Anti-Eotaxin3 antibody&#44; PA1451&#44; Western blotting<br>All lanes: Anti Eotaxin3 (PA1451) at 0.5ug/ml<br>Lane 1: COLO320 Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Predicted bind size: 11KD<br>Observed bind size: 11KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf9-antibody-pa1453-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1453-1-WB-anti-fgf9-antibody.jpgAnti-Fibroblast growth factor 9 FGF9 Antibody Picoband&reg;Anti-FGF9 antibody&#44; PA1453&#44; Western blotting<br>All lanes: Anti FGF9 (PA1453) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 23KD<br>Observed bind size: 23KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1453-2-IHC-anti-fgf9-antibody.jpgAnti-Fibroblast growth factor 9 FGF9 Antibody Picoband&reg;Anti-FGF9 antibody&#44; PA1453&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf10-antibody-pa1454-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1454-1-WB-anti-fgf10-antibody.jpgAnti-Fibroblast growth factor 10 FGF10 Antibody Picoband&reg;Anti-FGF10 antibody&#44; PA1454&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: A519 Cell Lysate<br>Lane 4: 293T Cell Lysate<br>Lane 5: HELA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf22-antibody-pa1455-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1455-1_1.jpgAnti-Fibroblast growth factor 22 FGF22 Antibody Picoband&reg; Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates&#44; <br> Lane 2: human Caco-2 whole cell lysates&#44; <br> Lane 3: human U-87MG whole cell lysates&#44; <br> Lane 4: human PC-3 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1455-2.jpgAnti-Fibroblast growth factor 22 FGF22 Antibody Picoband&reg; Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat testicular tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gm-csf-antibody-pa1456-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1456-1-WB-anti-gm-csf-antibody.jpgAnti-GM-CSF/CSF2 Antibody Picoband&reg;Anti-GM-CSF antibody&#44; PA1456&#44; Western blotting<br>All lanes: Anti GM-CSF (PA1456) at 0.5ug/ml<br>Lane 1: JURKAT Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: HT1080Whole Cell Lysate at 40ug<br>Predicted bind size: 16KD<br>Observed bind size: 35KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sftpa1-antibody-pa1458-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1458-sftpa1-primary-antibodies-wb-testing-1.jpgAnti-SFTPA1 Antibody Picoband&reg; Western blot analysis of SFTPA1 using anti-SFTPA1 antibody (PA1458). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody (Catalog # PA1458) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26-35 kDa. The expected band size for SFTPA1 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1458-2-IHC-anti-sftpa1-antibody.jpgAnti-SFTPA1 Antibody Picoband&reg; IHC analysis of SFTPA1 using anti-SFTPA1 antibody (PA1458).<br> SFTPA1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1 Antibody (PA1458) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1458-3-IHC-anti-sftpa1-antibody.jpgAnti-SFTPA1 Antibody Picoband&reg; IHC analysis of SFTPA1 using anti-SFTPA1 antibody (PA1458).<br> SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1 Antibody (PA1458) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcl16-antibody-pa1460-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1460-1-WB-anti-cxcl16-antibody.jpgAnti-C-X-C motif chemokine 16 CXCL16 Antibody Picoband&reg;Anti-CXCL16 antibody&#44; PA1460&#44; Western blotting<br>Lane 1: Recombinant Mouse CXCL16 Protein 10ng<br>Lane 2: Recombinant Mouse CXCL16 Protein 5ng<br>Lane 3: Recombinant Mouse CXCL16 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1460-2-IHC-anti-cxcl16-antibody.jpgAnti-C-X-C motif chemokine 16 CXCL16 Antibody Picoband&reg;Anti-CXCL16 antibody&#44; PA1460&#44; IHC(P)<br>IHC(P): Mouse Spleen Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dkk1-antibody-pa1462-boster.html2026-03-24T05:03:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1462-dkk1-primary-antibodies-wb-testing-1.jpgAnti-Dickkopf-related protein 1 DKK1 Antibody Picoband&reg; Western blot analysis of DKK1 using anti-DKK1 antibody (PA1462). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates&#44; <br> Lane 2: human A431 whole cell lysates&#44; <br> Lane 3: human Hela whole cell lysates&#44; <br> Lane 4: human PC-3 whole cell lysates&#44; <br> Lane 5: human Caco-2 whole cell lysates&#44; <br> Lane 6: human A549 whole cell lysates&#44; <br> Lane 7: rat spleen tissue lysates&#44; <br> Lane 8: mouse thymus tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # PA1462) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 39KD. The expected band size for DKK1 is at 29KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dld-antibody-pa1463-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1463-2-IHC-anti-dld-antibody.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg;Anti-DLD antibody&#44; PA1463&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1463-3-IF-anti-dld-antibody.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg;Anti-Lipoamide Dehydrogenase antibody&#44; PA1463&#44; ICC<br>ICC: MCF-7 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1463-dld-primary-antibodies-wb-testing-1.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; Western blot analysis of DLD using anti-DLD antibody (PA1463). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates,<br> Lane 4: human U251 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat heart tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLD antigen affinity purified polyclonal antibody (Catalog # PA1463) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLD at approximately 54 kDa. The expected band size for DLD is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1463-4.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; IF analysis of DLD using anti-DLD antibody (PA1463) <br> DLD was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-DLD Antibody (PA1463) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1463-dld-primary-antibodies-if-testing-5.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; IF analysis of DLD using anti-DLD antibody (PA1463). <br> DLD was detected in immunocytochemical section of HCT116 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-DLD Antibody (PA1463) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1463-dld-primary-antibodies-wb-testing-6.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; Western blot analysis of DLD using anti-DLD antibody (PA1463). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human SiHa whole cell lysates, <br> Lane 4: human U251 whole cell lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: rat heart tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLD antigen affinity purified polyclonal antibody (PA1463) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for DLD at approximately 54 kDa. The expected band size for DLD is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fadd-antibody-pa1464-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1464-fadd-primary-antibodies-wb-testing-1.jpgAnti-FADD Antibody Picoband&reg; Western blot analysis of FADD using anti-FADD antibody (PA1464). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HT1080 whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: human HepG2 whole cell lysates,<br> Lane 6: human Hela whole cell lysates,<br> Lane 7: human MCF-7 whole cell lysates,<br> Lane 8: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # PA1464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADD at approximately 28 kDa. The expected band size for FADD is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1464-fadd-primary-antibodies-wb-testing-2.jpgAnti-FADD Antibody Picoband&reg; Western blot analysis of FADD using anti-FADD antibody (PA1464). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates,<br> Lane 2: rat PC-12 whole cell lysates,<br> Lane 3: mouse spleen tissue lysates,<br> Lane 4: mouse kidney tissue lysates,<br> Lane 5: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADD antigen affinity purified polyclonal antibody (Catalog # PA1464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FADD at approximately 28 kDa. The expected band size for FADD is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gdnf-antibody-pa1465-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1465-12951_2022_1337_fig9_html.pngAnti-GDNF Antibody Picoband&reg;SPION-mediated magnetic actuation upregulates the expression of neurotrophic factors associated with repair phenotypes in Schwann cells. The protein expression of repair phenotype-related neurotrophic factors BDNF, GDNF, Olig1 and VEGF in different experimental groups was detected by immunohistochemical staining at 3 (a) , 7 (d) , 14 (g) and 21 days (j) after crush injury, and the protein expression levels were quantitatively analyzed ( b , e , h , k ). c , f , i , l The protein expression levels of such neurotrophic factors at the above time points were detected by ELISA, and the results were consistent with the immunohistochemical analysis. Each experiment was carried out in triplicate. The values are represented as the mean ± SD. Scale bar = 50 µm in panels a , d , g , j . * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-022-01337-5'>35351151</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1465-gdnf-primary-antibodies-wb-testing-1.jpgAnti-GDNF Antibody Picoband&reg; Western blot analysis of GDNF using anti-GDNF antibody (PA1465). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human GDNF protein 10 ng,<br> Lane 2: recombinant human GDNF protein 5 ng,<br> Lane 3: recombinant human GDNF protein 2.5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDNF antigen affinity purified polyclonal antibody (Catalog # PB9064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDNF at approximately 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1465-gdnf-primary-antibodies-ihc-testing-2.jpgAnti-GDNF Antibody Picoband&reg; IHC analysis of GDNF using anti-GDNF antibody (PA1465). <br> GDNF was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1465-gdnf-primary-antibodies-ihc-testing-3.jpgAnti-GDNF Antibody Picoband&reg; IHC analysis of GDNF using anti-GDNF antibody (PA1465). <br> GDNF was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1465-gdnf-primary-antibodies-ihc-testing-4.jpgAnti-GDNF Antibody Picoband&reg; IHC analysis of GDNF using anti-GDNF antibody (PA1465). <br> GDNF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GDNF Antibody (PA1465) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-2-antibody-pa1466-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1466-1-WB-anti-il-2-antibody.jpgAnti-Interleukin-2 IL2 Antibody Picoband&reg;Anti-IL-2 antibody&#44; PA1466&#44; Western blotting<br>Lane 1: Recombinant Mouse IL-2 Protein 10ng <br>Lane 2: Recombinant Mouse IL-2 Protein 5ng<br>Lane 3: Recombinant Mouse IL-2 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il7-antibody-pa1467-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1467-2-IHC-anti-il-7-antibody.jpgAnti-Interleukin-7 IL7 Antibody Picoband&reg;Anti-IL7 antibody&#44; PA1467&#44; IHC(P)<br>IHC(P): Mouse Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1467-1_1.jpgAnti-Interleukin-7 IL7 Antibody Picoband&reg; Western blot analysis of IL7 using anti-IL7 antibody (PA1467). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PA1467) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 20KD. The expected band size for IL7 is at 20KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-12-p40-antibody-pa1468-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1468-1-WB-anti-il-12-p40-antibody.jpgAnti-IL12 p40/IL12B Antibody Picoband&reg;Anti-IL-12(p40) antibody&#44; PA1468&#44; Western blotting<br>Lane 1: Recombinant Human IL-12 Protein 10ng<br>Lane 2: Recombinant Human IL-12 Protein 5ng<br>Lane 3: Recombinant Human IL-12 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-13-antibody-pa1469-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1469-1-WB-anti-il-13-antibody.jpgAnti-Interleukin-13 IL13 Antibody Picoband&reg;Anti-IL-13 antibody&#44; PA1469&#44; Western blotting<br>All lanes: Anti IL-13 (PA1469) at 0.5ug/ml<br>Lane 1: Recombinant Rat IL-13 Protein 5ng<br>Lane 2: Recombinant Rat IL-13 Protein 2.5ng<br>Predicted bind size: 13KD<br>Observed bind size: 13KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il23-p19-pa1470-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1470-1-WB-anti-il-23-p19-antibody.jpgAnti-IL23 P19/IL23A Antibody Picoband&reg;All lanes: Anti-IL23 P19 antibody&#44; PA1470<br> Lane 1: Recombinant Mouse IL-23 Protein 10ng<br> Lane 2: Recombinant Mouse IL-23 Protein 5ng<br> Lane 3: Recombinant Mouse IL-23 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppid-antibody-pa1472-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1472-ppid-primary-antibodies-wb-testing-1.jpgAnti-PPID Antibody Picoband&reg; Western blot analysis of PPID using anti-PPID antibody (PA1472). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human HEL whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human MOLT-4 whole cell lysates, <br> Lane 5: human PC-3 whole cell lysates, <br> Lane 6: human HL-60 whole cell lysates, <br> Lane 7: human THP-1 whole cell lysates, <br> Lane 8: rat liver tissue lysates. <br> Lane 9: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPID antigen affinity purified polyclonal antibody (Catalog # PA1472) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPID at approximately 38 kDa. The expected band size for PPID is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1472-2-IHC-anti-ppid-cyclophilin-40-antibody.jpgAnti-PPID Antibody Picoband&reg; IHC analysis of PPID using anti-PPID antibody (PA1472). <br> PPID was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PPID Antibody (PA1472) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-resistin-antibody-pa1473-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1473-1-WB-anti-resistin-antibody.jpgAnti-Resistin/RETN Antibody Picoband&reg;Anti-Resistin antibody&#44; PA1473&#44; Western blotting<br>Lane 1: Recombinant Mouse Resistin Protein 10ng<br>Lane 2: Recombinant Mouse Resistin Protein 5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-survivin-antibody-pa1474-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1474-birc5-primary-antibodies-wb-testing-1.jpgAnti-survivin/BIRC5 Antibody Picoband&reg; Western blot analysis of BIRC5 using anti-BIRC5 antibody (PA1474). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates,<br> Lane 2: human U20S whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 antigen affinity purified polyclonal antibody (Catalog # PA1474) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BIRC5 at approximately 16 kDa. The expected band size for BIRC5 is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fatty-acid-binding-protein-5-antibody-pa1475-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1475-1-WB-anti-fatty-acid-binding-protein-5-antibody.jpgAnti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband&reg;Anti-Fatty Acid Binding Protein 5 antibody&#44; PA1475&#44; Western blotting<br>All lanes: Anti Fatty Acid Binding Protein 5 (PA1475) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: HEPG2 Whole Cell Lysate at 40ug<br>Predicted bind size: 15KD<br>Observed bind size: 15KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1475-2-IHC-anti-fatty-acid-binding-protein-5-antibody.jpgAnti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband&reg;Anti-Fatty Acid Binding Protein 5 antibody&#44; PA1475&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf19-antibody-pa1476-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1476-1_3-WB-anti-fgf19-antibody.jpgAnti-Fibroblast growth factor 19 FGF19 Antibody Picoband&reg;Anti-FGF19 antibody&#44; PA1476&#44; Western blotting<br>All lanes: Anti FGF19 (PA1476) at 0.5ug/ml<br>Lane 1: U87 Whole Cell Lysate at 40ug<br>Lane 2: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 24KD<br>Observed bind size: 24KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1476-2-IHC-anti-fgf19-antibody.jpgAnti-Fibroblast growth factor 19 FGF19 Antibody Picoband&reg;Anti-FGF19 antibody&#44; PA1476&#44; IHC(P)<br>IHC(P): Human Gallbladder Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgfr1-antibody-pa1477-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1477-1-WB-anti-fgfr1-antibody.jpgAnti-FGFR1 Antibody Picoband&reg;Anti-FGFR1 antibody&#44; PA1477&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: SMMC Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br>Lane 5: MM231 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1477-2-IHC-anti-fgfr1-antibody.jpgAnti-FGFR1 Antibody Picoband&reg;Anti-FGFR1 antibody&#44; PA1477&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fos-b-antibody-pa1478-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1478-1-WB-anti-fos-b-antibody.jpgAnti-Fos B/FOSB Antibody Picoband&reg;Anti-Fos B antibody&#44; PA1478&#44; Western blotting<br>Lane 1: HT1080 Cell Lysate <br>Lane 2: SW620 Cell Lysate <br>Lane 3: HELA Cell Lysate <br>Lane 4: SMMC Cell Lysate <br>Lane 5: MM453 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adamts4-antibody-pa1479-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1479-1-WB-anti-adamts4-antibody.jpgAnti-ADAMTS4/ADAMTS4 Antibody Picoband&reg;Anti-ADAMTS4 antibody&#44; PA1479&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igfbp-1-antibody-pa1480-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1480-igfbp1-primary-antibodies-wb-testing-1.jpgAnti-IGFBP1 Antibody Picoband&reg; Western blot analysis of IGFBP1 using anti-IGFBP1 antibody (PA1480). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human U87 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP1 antigen affinity purified polyclonal antibody (Catalog # PA1480) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP1 at approximately 30 kDa. The expected band size for IGFBP1 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aimp2-p38-antibody-pa1481-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1481-aimp2-primary-antibodies-wb-testing-1.jpgAnti-AIMP2/p38 Antibody Picoband&reg; Western blot analysis of AIMP2/P38 using anti-AIMP2/P38 antibody (PA1481). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human U2OS whole cell lysates, <br> Lane 3: hman A549 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat lung tissue lysates, <br> Lane 6: mouse brain tissue lysates, <br> Lane 7: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIMP2/P38 antigen affinity purified polyclonal antibody (PA1481) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AIMP2/P38 at approximately 36 kDa. The expected band size for AIMP2/P38 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1481-2-IHC-anti-aimp2-p38-antibody.jpgAnti-AIMP2/p38 Antibody Picoband&reg; IHC analysis of AIMP2 using anti-AIMP2 antibody (PA1481). <br> AIMP2 was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIMP2 Antibody (PA1481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1481-3.jpgAnti-AIMP2/p38 Antibody Picoband&reg; IHC analysis of AIMP2 using anti-AIMP2 antibody (PA1481). <br> AIMP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIMP2 Antibody (PA1481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tau-antibody-pa1482-boster.html2026-03-24T05:03:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1482-1_1-WB-anti-tau-antibody.jpgAnti-Tau/MAPT Antibody Picoband&reg;Anti-Tau antibody&#44; PA1482&#44; Western blotting<br>All lanes: Anti (PA1482) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Lane 3: HT1080 Whole Cell Lysate at 40ug<br>Lane 4: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 79KD<br>Observed bind size: 79KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mta1-antibody-pa1483-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-mta1-primary-antibodies-wb-testing-1.jpgAnti-MTA1 Antibody Picoband&reg; Western blot analysis of MTA1 using anti-MTA1 antibody (PA1483). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human HELA whole cell lysates, <br> Lane 4: human PC-3 whole cell lysates, <br> Lane 5: human Raji whole cell lysates, <br> Lane 6: human K562 whole cell lysates, <br> Lane 7: rat brain tissue lysates, <br> Lane 8: mouse brain tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA1 antigen affinity purified polyclonal antibody (Catalog # PA1483) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTA1 at approximately 80KD. The expected band size for MTA1 is at 80KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-12894_2020_731_fig1_html.pngAnti-MTA1 Antibody Picoband&reg;The expression of MTA1 is up-regulated in RCCs. a Immunohistochemistry was used to characterize the expression of MTA1 in RCC and adjacent tissues. MTA1 was highly expressed in ccRCCs, compared to very weak staining in adjacent tissue. b Image Pro Plus was used for the statistical analysis of the positive signal (IOD) of MTA1 expression in ccRCCs and the adjacent tissue. The expression of MTA1 was significantly up-regulated in ccRCCs. ** p < 0.01; scale bar: 100 µm and 50 µm. c The expression of MTA1 in RCC cell lines. Normal renal cell line HEK293T and RCC cell lines A498 and 768-O cell lysates were used in western blotting analysis with MTA1 and GAPDH antibodies. The bands of MTA1 were from Additional file : and gapdh were from Additional file : <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12894-020-00731-1'>33059651</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-12894_2020_731_fig2_html.pngAnti-MTA1 Antibody Picoband&reg;MTA1 promotes A498 cell migration. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, cells in the four conditions were subjected to the wound healing assay. Macrographs were taken under ×100 magnification <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12894-020-00731-1'>33059651</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-12894_2020_731_fig3_html.pngAnti-MTA1 Antibody Picoband&reg;MTA1 enhances the invasion of A498 cells. a A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 24 h, the transwell cell invasion assay using A498 cells was performed, and macrographs were taken under ×40 magnification. Scale bar: 50 µm. b Image J was used to count the transmigrated cells and Student’s t test to analyze differences. ** p < 0.01; *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12894-020-00731-1'>33059651</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-12894_2020_731_fig4_html.pngAnti-MTA1 Antibody Picoband&reg;MTA1 regulated the expression of metastasis-related factors via the NFκB pathway. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. a Protein levels of MTA1; cells were collected for WB with MTA1 and GAPDH antibodies. The bands of over-expressed MTA1 were from Additional file : Fig. 2A and down-expressed MTA1 were from Additional file : Fig. 2B with arrow. b Cells were lysed and used in qRT-PCR assays to measure the E-cadherin mRNA expression. Statistical analysis was performed using Student’s t test. b , c A498 cells were transfected with pcDNA3.1-Flag and Flag-MTA1. After 36 h, the Flag-MTA1 group was treated with 10 nM PDTC for 12 h. Then cells were harvested for qRT-PCR evaluation to measure the mRNA expression of MMP2 ( b ) and MMP9 ( c ). Statistical analysis was performed using ANOVA. d A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, siNC, and si-MTA1. After 48 h, cells were subjected to western blotting analysis using antibodies targeting p-p65, p65 and ACTB. The bands of p-p65, p65 and ACTB were from Additional file : Fig. 3. e, f Image J was used to calculate the gray scanned bands of p-p65 ( b ) and p65 (C). * p < 0.05; ** p < 0.01; *** p < 0.001, **** p < 0.0001; NS not significant <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12894-020-00731-1'>33059651</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-12894_2020_731_fig5_html.pngAnti-MTA1 Antibody Picoband&reg;MTA1 regulated the migration and invasion of RCC cells via NF-κB. A498 cells were transfected with pcDNA3.1-Flag, Flag-MTA1, and Flag-MTA1 + PDTC (10 nM). a In the wound healing assay, MTA1 improved the migration of RCC cells, while the addition of the inhibitor PDTC blocked the effect of MTA1 on migration. Scale bar: 100×. b In the transwell assay, MTA1 markedly induced invasion of A498 cells, but compared to the MTA1 group, the invasion of MTA1 + PDTC A498 cells was lower. ** p < 0.01; *** p < 0.001; Scale bar: ×40 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12894-020-00731-1'>33059651</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1483-2-IHC-anti-mta1-antibody.jpgAnti-MTA1 Antibody Picoband&reg;Anti-MTA1 antibody&#44; PA1483&#44; IHC(P)<br>IHC(P): Human Rectal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-4_.jpgAnti-MTA1 Antibody Picoband&reg; IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). <br> MTA1 was detected in paraffin-embedded section of rat ovary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1483-3-IHC-anti-mta1-antibody.jpgAnti-MTA1 Antibody Picoband&reg;Anti-MTA1 antibody&#44; PA1483&#44; IHC(F)<br>IHC(F): Rat Ovary Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-5.jpgAnti-MTA1 Antibody Picoband&reg; IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). <br> MTA1 was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-6.jpgAnti-MTA1 Antibody Picoband&reg; IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). <br> MTA1 was detected in frozen section of mouse brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-mta1-primary-antibodies-ihc-testing-7_1.jpgAnti-MTA1 Antibody Picoband&reg; IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). <br> MTA1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-mta1-primary-antibodies-ihc-testing-8.jpgAnti-MTA1 Antibody Picoband&reg; IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). <br> MTA1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1483-mta1-primary-antibodies-ihc-testing-9.jpgAnti-MTA1 Antibody Picoband&reg; IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). <br> MTA1 was detected in paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr4-antibody-pa1484-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1484-1-WB-anti-tlr4-antibody.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Anti-TLR4 antibody&#44; PA1484&#44; Western blotting<br>Lane 1: HELA Cell Lysate <br>Lane 2: SMMC Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1484-2-IHC-anti-tlr4-antibody.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Anti-TLR4 antibody&#44; PA1484&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-40168_2019_761_fig3_html.pngAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;OTA promotes liver inflammation. a Relative mRNA expressions of TLR4, MYD88, IKBα, IL-6, and TNF-α in the liver after OTA oral gavage ( n = 6, mean with SEM). b Relative protein abundances of TLR4, MYD88, p-IKBα, p-IKBα/IKBα, and p-p65 in the liver after OTA oral gavage ( n = 6, mean with SEM). c Effect of OTA on levels of liver cytokines, including IL-1β, IL-6, TNF-α, IL-8, IL-10, and IFN-γ ( n = 6, mean with SEM). d Representative images of H&E-stained liver sections in CON and OTA group (magnification × 400, scale bar 100 μm, n = 6). e Statistical analysis of the percentage of inflammatory cells in different groups shown in d ( n = 6, mean with SEM). f Effect of OTA on serum levels of AST, ALT, ALP, and LDH ( n = 6, mean with SEM). g Serum LPS level with or without OTA treatment ( n = 6, mean with SEM). h Effect of OTA on serum levels of IL-1β, IL-6, TNF-α, and IL-10 ( n = 6, mean with SEM). Data in a , b , c , e , f , g , and h were analyzed with unpaired t test, * P < 0.05; ** P < 0.01, *** P < 0.001, P <0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40168-019-0761-z'>31779704</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-40168_2019_761_fig5_html.pngAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;OTA fails to promote liver inflammation in antibiotics-treated ducks. a Liver LPS level in different groups ( n = 6, mean with SEM). b Relative mRNA expressions of TLR4, MYD88, IKBα, IL-6, and TNF-α in the liver of antibiotics-treated ducks with or without OTA ( n = 6, mean with SEM). c Levels of IL-1β, IL-6, TNF-α, IL-8, IL-10, and IFN-γ in the liver of antibiotics-treated ducks with or without OTA ( n = 6, mean with SEM). d Representative images of H&E-stained liver sections (magnification × 400; scale bar 100 μm; n = 6). e Statistical analysis of the percentage of inflammatory cells in different groups shown in d ( n = 6, mean with SEM). f Effect of OTA on serum levels of AST, ALT, ALP and LDH in antibiotics-treated ducks ( n = 6, mean with SEM). g Serum LPS level with or without OTA treatment in antibiotics-treated ducks ( n = 6, mean with SEM). h Effect of OTA on serum levels of IL-1β, IL-6, TNF-α, and IL-10 ( n = 6, mean with SEM). Data were analyzed with unpaired t test. n.s., not significant <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40168-019-0761-z'>31779704</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-40168_2019_761_fig7_html.pngAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;OTA-originated microbiota induces liver inflammation. a Liver LPS level in FMT (CON) and FMT (OTA) ducks. b Relative mRNA expressions of TLR4, MYD88, IKBα, IL-6, and TNF-α in the liver after FMT ( n = 6, mean with SEM). c Relative protein abundance of TLR4, MYD88, p-IKBα, p-IKBα/IKBα, and p-p65 in the liver after FMT ( n = 6, mean with SEM). d Effect of FMT on the liver levels of IL-1β, IL-6, TNF-α, and IL-10 ( n = 6, mean with SEM). e Representative H&E-stained liver sections. f Statistical analysis of the percentage of inflammatory cells in different groups shown in e ( n = 6, mean with SEM). g Serum levels of AST, ALT, ALP, and LDH in different groups ( n = 6, mean with SEM). h Serum LPS level in different FMT groups ( n = 6, mean with SEM). i Serum levels of IL-1β, IL-6, and TNF-α after FMT ( n = 6, mean with SEM). FMT (CON): ducks received the CON group fecal microbiota. FMT (OTA): ducks received the OTA group fecal microbiota. Data were analyzed with unpaired t test, n = 6. * P < 0.05; ** P < 0.01, *** P < 0.001, **** P <0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40168-019-0761-z'>31779704</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-thnov07p2537g001.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Effect of LPS on Rab26 and TLR4 expression in HPMVECs. A. Rab26 and TLR4 proteins expression was detected by western blot analyses after HPMVECs were treated with the indicated doses of LPS for 24 h. The upper, middle and lower panels show representative bands of Rab26, TLR4 and β-actin proteins, respectively. B. The histograms show the quantitative analysis of the Rab26 and TLR4 levels, which were normalized to the β-actin levels. *, p < 0.05 versus the LPS 0 μg/mL group. C. TLR4 surface expression in HPMVECs was analyzed by FACS analysis after the cells were incubated with LPS at the indicated dose for 24 h. D. The histograms show the mean fluorescence intensity (MFI) of surface TLR4 staining. *, p < 0.05 versus the control group. ^, p < 0.05 versus the LPS 0.1 μg/mL group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5525755/&orig_host=www.ncbi.nlm.nih.gov'>28744333</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-thnov07p2537g007.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Effect of Rab26 on the TLR4 signaling pathway in HPMVECs. A. HPMVECs were cultured in 10% serum ECM for 24 h and then divided into six groups. The first group was cultured in fresh ECM for 60 h as control. Two groups were transfected with 50 nM siRab26-DYM for 12 h, then cultured in fresh ECM for another 24 h, and treated with or without 1 μg/mL LPS for 24 h. Two groups were infected with MOI100 Adv. Rab26 for 6 h, then cultured in fresh ECM for 24 h, and treated with or without 1 μg/mL LPS for 24 h. The last group was cultured in fresh ECM for 36 h and treated with 1 μg/mL LPS for 24 h. All cells were collected for western blotting detection. These panels show representative bands of the Rab26, TLR4, MyD88, TRAF6, NF-κB p65 and β-actin proteins. B. The histograms show the quantitative analysis of the Rab26 and TLR4 levels, which were normalized to the β-actin levels. *, p < 0.05 versus the control group. ^, p < 0.05 versus the LPS group. C. Quantitative analysis of MyD88, TRAF6 and NF-κB p65 levels, which were normalized to β-actin levels. *, p < 0.05 versus the control group. ^, p < 0.05 versus the LPS group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5525755/&orig_host=www.ncbi.nlm.nih.gov'>28744333</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-thnov07p2537g006.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Effects of Rab26 on cell surface TLR4 in HPMVECs . A. FACS analysis of the surface level of TLR4. Cells were first cultured in 10% serum ECM for 24 h and then divided into five groups. Two groups were transfected with 50 nM siRab26-DYM or 50 nM siNC-DYM for 12 h; two other groups were infected with an MOI of 100 of Adv. Rab26 or Adv. Vector for 6 h. Then all cells were culture in fresh ECM for 48 h, collected respectively and analyzed using FACS analysis. The histograms show the mean fluorescence intensity of surface TLR4 staining. The data are presented as the means ± S.E. of three separate experiments (n = 3) *, p< 0.05 versus the control group. B. TLR4 expression on the HPMVEC surface when cells were treated with 1 μg/mL LPS for 24 h. Cells were first cultured in 10% serum ECM for 24 h and divided into six groups. Two group were transfected with 50 nM siRab26-DYM or 50 nM siNC-DYM for 12 h and cultured in fresh ECM for 24 h, then treated with 1 μg/mL LPS for 24 h; two other groups were infected with MOI100 Adv. Rab26 or MOI100 Adv. Vector for 6 h and cultured in fresh ECM for 24 h, then treated with 1 μg/mL LPS for 24 h, The last group was cultured in fresh ECM for 36 h and treated with 1 μg/mL LPS for 24 h. All groups were then collected respectively and subjected to FACS analysis. The histograms show the mean fluorescence intensity of surface TLR4 staining. The data are representative of three separate experiments (mean ± S.E., n = 3). *, p < 0.05 versus the control group; ^, p < 0.05 versus the LPS group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5525755/&orig_host=www.ncbi.nlm.nih.gov'>28744333</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-fncel-11-00099-g004.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Effect of Prdx6-iPLA2 on Toll-like receptor 2 (TLR2) and TLR4 expression in microglia in response to OGD/R. TLR2 and TLR4 were activated during OGD/R exposure. Prdx6-iPLA2 siRNA or MJ33 treatment could inhibit TLR2 and TLR4 mRNA (A,B) and protein expression (C–E) . Results are expressed as the mean ± SEM of three independent experiments. ** p < 0.01 vs. Control; # p < 0.05 vs. Scramble; ## p < 0.01 vs. Scramble.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2017.00099/full'>28424593</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-fncel-11-00099-g009.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Expression of TLR2 and TLR4 in response to Prdx6 siRNA and MJ33. Real-time PCR showed that TLR2 (A) and TLR4 (B) expression increased after MCAO or giving Prdx6-siRNA. Combined treatment with Prdx6-siRNA and MJ33 downregulated TLR2 and TLR4 levels. Representative Western blot (C) and semi-quantitative analyses of the levels of TLR2 (D) and TLR4 (E) in the cortex after MCAO. Results are expressed as the mean ± SEM of three independent experiments. & p < 0.01 vs. Sham; ** p < 0.01 vs. Scramble; # p < 0.05, vs. Prdx6 siRNA; ## p < 0.01 vs. Prdx6 siRNA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2017.00099/full'>28424593</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1484-thnov07p2537g008.jpgAnti-Toll-like receptor 4 TLR4 Antibody Picoband&reg;Rab26 regulated the nuclear translocation of Foxo1 in HPMVECs. A. HPMVECs were cultured in 10% serum ECM for 24 h and then divided into six groups. One group was cultured in fresh ECM for 60 h as control. Two groups were transfected with 50 nM siRab26-DYM for 12 h, then cultured in fresh ECM for 24 h, and treated with or without 1 μg/mL LPS for 24 h. Two groups were infected with MOI100 Adv. Rab26 for 6 h, then culture in fresh ECM for 24 h, and treated with or without 1 μg/mL LPS for 24 h. The last group was cultured in fresh ECM for 36 h and directly treated with 1 μg/mL LPS for 24 h. All cells were collected for Western blotting detection. Five panels show representative bands of p-Foxo1, T-Foxo1, β-actin, N-Foxo1 and HRPT. B. Quantitative analysis of p-Foxo1, T-Foxo1 levels, which were normalized to β-actin levels. *, p < 0.05 versus the control group. ^, p < 0.05 versus the LPS group. C. Quantitative analysis of N-Foxo1 levels, which were normalized to HRPT levels. *, p < 0.05 versus the control group. ^, p < 0.05 versus the LPS group. D. HPMVECs were cultured in 10% serum ECM for 24 h waiting for cell attachment and then divided into three groups. The first group was cultured in fresh ECM for 60 h as control. The second group was transfected with 50 nM siRab26-DYM for 12 h, then cultured in fresh ECM for 48 h. The last group was treated with Foxo1 siRNA for 12 h first, and then transfected with 50 nM siRab26-DYM, then cultured in fresh ECM for 24 h. All cells were collected as sample for Western blotting detection. Panels show representative bands of Rab26, p-Foxo1, T-Foxo1, TLR4, β-actin, N-Foxo1, HRPT. E. Quantitative analysis of Rab26, p-Foxo1, T-Foxo1, TLR4 levels, which were normalized to β-actin levels. *, p < 0.05 versus the control group. ^, p < 0.05 versus the siRab26-DYM group. F. Quantitative analysis of N-Foxo1 levels, which were normalized to HRPT levels. *, p < 0.05 versus the control group. ^, p < 0.05 versus the siRab26-DYM group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5525755/&orig_host=www.ncbi.nlm.nih.gov'>28744333</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-amylase-1-antibody-pa1486-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1239-amy1a-primary-antibodies-wb-testing-1.jpgAnti-Alpha Amylase 1/AMY1A Antibody Picoband&reg;Figure 1. Western blot analysis of Alpha Amylase 1 using anti-Alpha Amylase 1 antibody (PA1486). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human PANC-1 whole cell lysates,<br> Lane 2: human pancreatic cancer tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Amylase 1 antigen affinity purified polyclonal antibody (Catalog # PA1486) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Amylase 1 at approximately 55-58 kDa. The expected band size for Alpha Amylase 1 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angptl1-antibody-pa1487-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1487-1-WB-anti-angptl1-antibody.jpgAnti-Angiopoietin-related protein 1 ANGPTL1 Antibody Picoband&reg;Anti-ANGPTL1 antibody&#44; PA1487&#44; Western blotting<br>Lane 1: A549 Cell Lysate<br>Lane 2: SW620 Cell Lysate<br>Lane 3: MCF-7 Cell Lysate<br>Lane 4: MM231 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-3-antibody-pa1488-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-wb-testing-1_2.jpgAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; Western blot analysis of AQP3 using anti-AQP3 antibody (PA1488). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: rat kidney tissue lysates, <br> Lane 3: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP3 antigen affinity purified polyclonal antibody (PA1488) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AQP3 at approximately 33 kDa. The expected band size for AQP3 is at 31.5 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-wb-testing-2.pngAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; Western blot analysis of AQP3 using anti-AQP3 antibody (PA1488). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: normal group-rat colon tissue lysates, <br> Lane 2: control group-rat colon tissue lysates, <br> Lane 3: traditional Chinese medicine treatment-rat colon tissue lysates, <br> Lane 4: Western medicine treatment-rat colon tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP3 antigen affinity purified polyclonal antibody (PA1488) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for AQP3 at approximately 35 kDa. The expected band size for AQP3 is at 31.5 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-ihc-testing-2_2.jpgAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; IHC analysis of AQP3 using anti-AQP3 antibody (PA1488). <br> AQP3 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP3 Antibody (PA1488) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-ihc-testing-3_2.jpgAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; IHC analysis of AQP3 using anti-AQP3 antibody (PA1488). <br> AQP3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP3 Antibody (PA1488) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-ihc-testing-4_2.jpgAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; IHC analysis of AQP3 using anti-AQP3 antibody (PA1488). <br> AQP3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP3 Antibody (PA1488) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-ihc-testing-5.jpgAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; IHC analysis of AQP3 using anti-AQP3 antibody (PA1488). <br> AQP3 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP3 Antibody (PA1488) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-if-testing-6_1_1.jpgAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; IF analysis of AQP3 using anti-AQP3 antibody (PA1488). <br> AQP3 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- AQP3 Antibody (PA1488) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1488-aqp3-primary-antibodies-fcm-testing-7.pngAnti-Aquaporin 3/AQP3 Antibody Picoband&reg; Flow Cytometry analysis of RT4 cells using anti-AQP3 antibody (PA1488). <br> Overlay histogram showing RT4 cells stained with PA1488 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP3 Antibody (PA1488, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aromatase-antibody-pa1489-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1489-1-WB-anti-aromatase-antibody.jpgAnti-Aromatase/CYP19A1 Antibody Picoband&reg;Anti-Aromatase antibody&#44; PA1489&#44; Western blotting<br>Lane 1: Human Placenta Tissue Lysate<br>Lane 2: Human Placenta Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1489-2-IHC-anti-aromatase-antibody.jpgAnti-Aromatase/CYP19A1 Antibody Picoband&reg;Anti-Aromatase antibody&#44; PA1489&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-4-antibody-pa1490-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1490-1-WB-anti-caspase-4-antibody.jpgAnti-Caspase 4/CASP4 Antibody Picoband&reg;Anti-Caspase 4 antibody&#44; PA1490&#44; Western blotting<br>All lanes: Anti Caspase 4 (PA1490) at 0.5ug/ml<br> Lane 1: MCF-7 Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: JURKAT Whole Cell Lysate at 40ug<br> Lane 4: CEM Whole Cell Lysate at 40ug<br> Lane 5: SW620 Whole Cell Lysate at 40ug<br> Predicted bind size: 43KD<br> Observed bind size: 20KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1490-2-IHC-anti-caspase-4-antibody.jpgAnti-Caspase 4/CASP4 Antibody Picoband&reg;Anti-Caspase 4 antibody&#44; PA1490&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gpr2-ccr10-antibody-pa1491-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1491-1-WB-anti-gpr2-ccr10-antibody.jpgAnti-GPR2/CCR10 Antibody Picoband&reg;Anti-GPR2/CCR10 antibody&#44; PA1491&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: SW620 Cell Lysate<br>Lane 3: A549 Cell Lysate<br>Lane 4: MM231 Cell Lysate<br>Lane 5: SMMC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ifn-gamma-antibody-pa1493-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1493-1-WB-anti-ifn-gamma-antibody.jpgAnti-Interferon gamma/IFNG Antibody Picoband&reg;Anti-IFN gamma antibody&#44; PA1493&#44; Western blotting<br>All lanes: Anti IFN gamma (PA1493) at 0.5ug/ml<br>Lane 1: Recombinant Human IFN gamma Protein 10ng<br>Lane 2: Recombinant Human IFN gamma Protein 5ng<br>Lane 3: Recombinant Human IFN gamma Protein 2.5ng<br>Lane 4: Recombinant Human IFN gamma Protein 1.25ng<br>Predicted bind size: 19KD<br>Observed bind size: 19KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ape1-antibody-pa1494-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-wb-testing-1.jpgAnti-APE1/APEX1 Antibody Picoband&reg; Western blot analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human RT4 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse HEPA1-6 whole cell lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates,<br> Lane 9: mouse ANA-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody (Catalog # PA1494) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-ihc-testing-2.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-ihc-testing-3.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-ihc-testing-4.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-ihc-testing-5.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-ihc-testing-6.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-if-testing-7.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IF analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-if-testing-8.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IF analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. DyLight 647 Conjugated Goat Anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1494-apex1-primary-antibodies-if-testing-9.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IF analysis of APEX1 using anti-APEX1 antibody (PA1494). <br> APEX1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-APEX1 Antibody (PA1494) overnight at 4°C. DyLight 647 Conjugated Goat Anti-rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igfbp-3-antibody-pa1498-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1498-1-WB-anti-igfbp-3-antibody.jpgAnti-IGFBP3 Antibody Picoband&reg;Anti-IGFBP-3 antibody&#44; PA1498&#44; Western blotting<br>All lanes: Anti IGFBP-3 (PA1498) at 0.5ug/ml<br>Lane 1: 293T Whole Cell Lysate at 40ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Lane 3: A549 Whole Cell Lysate at 40ug<br>Lane 4: SW620 Whole Cell Lysate at 40ug<br>Predicted bind size: 32KD<br>Observed bind size: 40KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1498-2_1.jpgAnti-IGFBP3 Antibody Picoband&reg; Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (PA1498). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates&#44; <br> Lane 2: rat kidney tissue lysates&#44; <br> Lane 3: rat heart tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (Catalog # PA1498) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 40KD. The expected band size for IGFBP3 is at 32KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mekk2-antibody-pa1499-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1499-1_1.jpgAnti-MEKK2/MAP3K2 Antibody Picoband&reg; Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates<br> Lane 2: human K562 whole cell lysates<br> Lane 3: human THP-1 whole cell lysates<br> Lane 4: human Raji whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1499-2.jpgAnti-MEKK2/MAP3K2 Antibody Picoband&reg; Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates<br> Lane 2: rat brain tissue lysates<br> Lane 3: rat heart tissue lysates<br> Lane 4: rat C6 whole cell lysates<br> Lane 5: mouse kidney tissue lysates<br> Lane 6: mouse brain tissue lysates<br> Lane 7: mouse heart tissue lysates<br> Lane 8: mouse Neuro-2a whole cell lysates<br> Lane 9: mouse NIH/3T3 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apelin-antibody-pa1501-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1501-1-WB-anti-apelin-antibody.jpgAnti-Apelin/APLN Antibody Picoband&reg;Anti-Apelin antibody&#44; PA1501&#44; Western blotting<br>Working concentration of primary antibody: 0.5 μg/ml; 40μg protein was loaded. <br>Lane 1: U87 Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: MM453 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcat2-antibody-pa1502-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1502-bcat2-primary-antibodies-wb-testing-1.jpgAnti-BCAT2 Antibody Picoband&reg;Western blot analysis of BCAT2 using anti-BCAT2 antibody (PA1502). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: monkey COS-7 whole cell lysates,<br> Lane 5: human RT4 whole cell lysates,<br> Lane 6: human 293T whole cell lysates,<br> Lane 7: human A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT2 antigen affinity purified polyclonal antibody (PA1502) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCAT2 at approximately 41 kDa. The expected band size for BCAT2 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1502-bcat2-primary-antibodies-ihc-testing-1.jpgAnti-BCAT2 Antibody Picoband&reg;IHC analysis of BCAT2 using anti-BCAT2 antibody (PA1502). <br>BCAT2 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT2 Antibody (PA1502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1502-bcat2-primary-antibodies-ihc-testing-2.jpgAnti-BCAT2 Antibody Picoband&reg;IHC analysis of BCAT2 using anti-BCAT2 antibody (PA1502). <br>BCAT2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT2 Antibody (PA1502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1502-bcat2-primary-antibodies-if-testing-1.jpgAnti-BCAT2 Antibody Picoband&reg;IF analysis of BCAT2 using anti-BCAT2 antibody (PA1502). <br> BCAT2 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BCAT2 Antibody (PA1502) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1502-bcat2-primary-antibodies-fcm-testing-1.jpgAnti-BCAT2 Antibody Picoband&reg;Flow Cytometry analysis of MCF-7 cells using anti-BCAT2 antibody (PA1502). <br> Overlay histogram showing MCF-7 cells stained with PA1502 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCAT2 Antibody (PA1502, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcl1-antibody-pa1503-boster.html2026-03-24T05:03:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1503-1-WB-anti-mcl1-antibody.jpgAnti-MCL1 Antibody Picoband&reg;Anti-MCL1 antibody&#44; PA1503&#44; Western blotting<br>All lanes: Anti MCL1 (PA1503) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 37KD<br> Observed bind size: 37KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcl10-antibody-pa1504-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1504-1-WB-anti-bcl10-antibody.jpgAnti-B-cell lymphoma/leukemia 10 Bcl10 Antibody Picoband&reg;Anti-Bcl10 antibody&#44; PA1504&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: COLO320 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bone-sialoprotein-antibody-pa1505-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1505-1_1-IHC-anti-bone-sialoprotein-antibody.jpgAnti-Bone Sialoprotein/IBSP Antibody Picoband&reg;Anti-Bone Sialoprotein antibody&#44; PA1505&#44; IHC(P)<br>IHC(P): Human Osteosarcoma Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1505-41368_2020_88_fig4_html.pngAnti-Bone Sialoprotein/IBSP Antibody Picoband&reg;Analysis of the mRNA and protein expression of osteogenic differentiation markers during the osteogenic differentiation of DFCs. a Histograms showing the relative mRNA levels of ALP, BSP, OCN , and RUNX2 during the induction of differentiation. The mRNA levels were determined using RT-PCR with GAPDH as a reference for normalization. b Representative western blots showing the protein levels of ALP, BSP, OCN, and RUNX2. c Histograms showing the relative protein levels of ALP, BSP, OCN, and RUNX2 during the induction of differentiation. Values are means ± SD of three independent experiments (* P < 0.05 and ** P < 0.01) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41368-020-00088-z'>32737282</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bob1-antibody-pa1506-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1506-1-WB-anti-bob1-antibody.jpgAnti-BOB1/POU2AF1 Antibody Picoband&reg;Anti-BOB1 antibody&#44; PA1506&#44; Western blotting<br>Lane: Rat Spleen Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1506-2-IHC-anti-bob1-antibody.jpgAnti-BOB1/POU2AF1 Antibody Picoband&reg;Anti-BOB1 antibody&#44; PA1506&#44; IHC(P)<br>IHC(P): Rat Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ribonuclease-a-antibody-pa1507-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1507-1-WB-anti-ribonuclease-a-antibody.jpgAnti-Ribonuclease A/RNASE1 Antibody Picoband&reg;Anti-Ribonuclease A antibody&#44; PA1507&#44; Western blotting<br>All lanes: Anti Ribonuclease A (PA1507) at 0.5ug/ml<br>Lane 1: MCF-7 Whole Cell Lysate at 40ug<br>Lane 2: U87 Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Lane 4: MM231 Whole Cell Lysate at 40ug<br>Lane 5: JURKAT Whole Cell Lysate at 40ug<br>Lane 6: SMMC Whole Cell Lysate at 40ug<br>Lane 7: COLO320 Whole Cell Lysate at 40ug<br>Lane 8: SW620 Whole Cell Lysate at 40ug<br>Lane 9: CEM Whole Cell Lysate at 40ug<br>Predicted bind size: 18KD<br>Observed bind size: 18KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat1-antibody-pa1508-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1508-stat1-primary-antibodies-wb-testing-1.jpgAnti-STAT1 Antibody Picoband&reg; Western blot analysis of STAT1 using anti-STAT1 antibody (PA1508). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human Daudi whole cell lysates,<br> Lane 5: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # PA1508) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91 kDa. The expected band size for STAT1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1508-stat1-primary-antibodies-fcm-testing-2_1.jpgAnti-STAT1 Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-STAT1 antibody (PA1508). <br>Overlay histogram showing U251 cells stained with PA1508 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (PA1508, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a3-antibody-pa1510-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1510-anxa3-primary-antibodies-wb-testing-1.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; Western blot analysis of ANXA3 using anti-ANXA3 antibody (PA1510). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human CACO-2 whole cell lysates.<br> Lane 2: human Hela whole cell lysates.<br> Lane 3: human A431 whole cell lysates.<br> Lane 4: human SH-SY5Y whole cell lysates.<br> Lane 5: rat lung tissue lysates.<br> Lane 6: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA3 antigen affinity purified polyclonal antibody (Catalog # PA1510) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANXA3 at approximately 36 kDa. The expected band size for ANXA3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1510-anxa3-primary-antibodies-ihc-testing-2.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of ANXA3 using anti-ANXA3 antibody (PA1510). <br> ANXA3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANXA3 Antibody (PA1510) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1510-anxa3-primary-antibodies-ihc-testing-3.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of ANXA3 using anti-ANXA3 antibody (PA1510). <br> ANXA3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANXA3 Antibody (PA1510) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-8-antibody-pa1511-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1511-1-WB-anti-aquaporin-8-antibody.jpgAnti-Aquaporin 8/AQP8 Antibody Picoband&reg; Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1511-aqp8-primary-antibodies-wb-testing-2.jpgAnti-Aquaporin 8/AQP8 Antibody Picoband&reg; Western blot analysis of AQP8 using anti-AQP8 antibody (PA1511). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP8 antigen affinity purified polyclonal antibody (Catalog # PA1511) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP8 at approximately 27KD. The expected band size for AQP8 is at 27KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xiap-antibody-pa1512-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1512-2-IHC-anti-xiap-antibody.jpgAnti-XIAP Antibody Picoband&reg;Anti-XIAP antibody&#44; PA1512&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1512-1_1.jpgAnti-XIAP Antibody Picoband&reg; Western blot analysis of XIAP using anti-XIAP antibody (PA1512). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysate&#44; <br> Lane 2: human Raji whole cell lysate&#44; <br> Lane 3: rat C6 whole cell lysate&#44; <br> Lane 4: mouse Neuro-2a whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XIAP antigen affinity purified polyclonal antibody (Catalog # PA1512) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XIAP at approximately 57KD. The expected band size for XIAP is at 57KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-10-antibody-pa1513-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1513-1-WB-anti-caspase-10-antibody.jpgAnti-Caspase-10/CASP10 Antibody Picoband&reg;Anti-Caspase-10 antibody&#44; PA1513&#44; Western blotting<br>Lane 1: COLO320 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: SW620 Cell Lysate<br>Lane 4: RAJI Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caveolin-1-antibody-pa1514-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1514-cav1-primary-antibodies-wb-testing-1.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PA1514). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human Hacat whole cell lysates,<br> Lane 4: human U87 whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-1/CAV1 antigen affinity purified polyclonal antibody (Catalog # PA1514) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-1/CAV1 at approximately 22 kDa. The expected band size for Caveolin-1/CAV1 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1514-cav1-primary-antibodies-ihc-testing-2.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PA1514). <br> Caveolin-1/CAV1 was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caveolin-1/CAV1 Antibody (PA1514) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1514-cav1-primary-antibodies-fcm-testing-4.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-Caveolin-1/CAV1 antibody (PA1514). <br>Overlay histogram showing A549 cells stained with PA1514 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1/CAV1 Antibody (PA1514, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1514-cav1-primary-antibodies-if-testing-3.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PA1514). <br> Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Caveolin-1/CAV1 Antibody (PA1514) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr4-antibody-pa1517-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1517-1-WB-anti-ccr4-antibody.jpgAnti-C-C chemokine receptor type 4 CCR4 Antibody Picoband&reg;Anti-CCR4 antibody&#44; PA1517&#44; Western blotting<br>All lanes: Anti CCR4 (PA1517) at 0.5ug/ml<br>Lane 1: Rat Thymus Tissue Lysate at 50ug<br>Lane 2: Rat Spleen Tissue Lysate at 50ug<br>Lane 3: HEPA Whole Cell Lysate at 40ug<br>Predicted bind size: 41KD<br>Observed bind size: 41KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd68-antibody-pa1518-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-wb-testing-1_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; Western blot analysis of CD68 using anti-CD68 antibody (PA1518). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates,<br> Lane 2: mouse spleen tissue lysates,<br> Lane 3: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-ihc-testing-3_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; IHC analysis of CD68 using anti-CD68 antibody (PA1518). <br> CD68 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (PA1518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-ihc-testing-2_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; IHC analysis of CD68 using anti-CD68 antibody (PA1518). <br> CD68 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (PA1518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-ihc-testing-4_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; IHC analysis of CD68 using anti-CD68 antibody (PA1518). <br> CD68 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (PA1518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-ihc-testing-5_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; IHC analysis of CD68 using anti-CD68 antibody (PA1518). <br> CD68 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (PA1518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-ihc-testing-6_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; IHC analysis of CD68 using anti-CD68 antibody (PA1518). <br> CD68 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (PA1518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-ihc-testing-7.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; IHC analysis of CD68 using anti-CD68 antibody (PA1518). <br> CD68 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (PA1518) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-if-testing-8_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; IF analysis of CD68 using anti-CD68 antibody (PA1518). <br> CD68 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD68 Antibody (PA1518) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127)) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-cd68-primary-antibodies-fcm-testing-9_1.jpgAnti-Macrosialin CD68 Antibody Picoband&reg; Flow Cytometry analysis of RAW264.7 cells using anti-CD68 antibody (PA1518). <br> Overlay histogram showing RAW264.7 cells stained with PA1518 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD68 Antibody (PA1518, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fphar-16-1649902-g009.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;CS attenuates MMT in RIF rats. (A) Co-staining of the α-SMA (green) with CD68 (red) by Double Immunofluorescence. (B) Co-localization analysis of CD68+α-SMA + MMT cells. (C) Staining intensity of CD68+α-SMA + MMT cells was quantified. (D) Co-staining of the α-SMA (green) with CD206 (red) by Double Immunofluorescence. (E) Co-localization analysis of CD206+α-SMA + MMT cells. (F) Staining intensity of CD206+α-SMA + MMT cells was quantified (n = 4). (G) mRNA Expression of AKT1, EGFR, IL-6, and IL-10 in RIF rats kidney (n = 3). ## p < 0.01 vs. the sham group; * p < 0.05 vs. the model group; ** p < 0.01 vs. the model group. Scale bars (40 μm, 10 μm).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1649902/full'>41142245</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518_genes-16-00817-g007.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Super-enhancer analysis reveals potential targets in SPP1+ TAMs. (A) Immunofluorescence staining of CD68 (yellow) and SPP1 (green) in liver tissues from control and HCC mice (n = 3 per group). Quantification shows significant increase in SPP1+ TAMs in HCC. Data are shown as mean ± SD; *** p < 0.001. (B) Heatmap showing normalized signal intensity of 133 super-enhancers (rows) across seven macrophage subtypes (columns), revealing subtype-specific enrichment in SPP1+ TAMs. Each row represents one SE region, and each column corresponds to one TAM subtype. (C) Correlation between SE signal and SPP1 expression. R and p-values were calculated by Pearson correlation. (D) Motif enrichment analysis of TFs on peaks within SE region. The TFs were ranked based on statistical significance using hypergeometric testing. (E) TF–SE–target gene regulatory network in SPP1+ TAMs. Nodes in red represent TFs, nodes in blue represent SEs, and nodes in green represent SE target genes. Node size of TFs corresponds to the significance of motif enrichment within SE-associated peaks, measured as −log10(P) from hypergeometric testing. Gene nodes labeled in red indicate known markers of SPP1+ TAMs. Inner edges represent regulatory association between each TF and SE calculated by linking scores. Outer edges reflect the correlation between chromatin accessibility and expression. (F) Structural modeling of the SPI1 protein bound to the SE region that regulates SPP1 gene, as predicted by AlphaFold3 and PDBePISA. (G) Drug–gene interaction analysis of SE target genes in (E). The y-axis indicates druggability score from DGIdb. Dot size reflects the number of drug interactions, and dot color indicates hazard ratio in HCC.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.mdpi.com/2073-4425/16/7/817'>40725473</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-gr2.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;DSS-induced colitis in Dhpsfl/fl and DhpsΔmye mice. Animals were treated or not with 4 % DSS. Expression of CD68 (red) and DHPS (green) (A) or CD68 (red) and EIF5AHyp (green) (B) was assessed in the colon by IF; merged, yellow; nucleus, blue. These pictures are representative of staining performed on 3 control mice and 4–5 DSS-treated mice per genotype. Body weights were measured daily and are shown as percentage of initial body weight (C); *P < 0.05 versus Dhpsfl/fl mice + DSS and §P < 0.05, §§§P < 0.001 compared to DSS-treated DhpsΔmye mice, by two-way ANOVA and Tukey test. At sacrifice, colons were dissected, washed, weighed, and measured (D). Swiss-rolls were stained with H&E (E; scale bars, 50 μm) and analyzed to determine the histologic injury score (F). In (D) and (F), ***P < 0.001, ****P < 0.0001 by one-way ANOVA and Tukey test; Dhpsfl/fl, n = 5; DhpsΔmye, n = 4; Dhpsfl/fl + DSS, n = 18; DhpsΔmye + DSS, n = 18. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)09869-4'>39027559</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518_fnut-12-1649407-g005.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Subcellular localization of ABCA1 in lung tissue. (A) Co-staining of ABCA1 (red) and SFTPC (green, alveolar type II marker); nuclei stained with DAPI (blue). (B) Co-staining of ABCA1 (red) and CD68 (green, macrophage marker) to determine cell-type-specific expression. n = 3–5 mice/group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1649407/full'>40808841</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518_fimmu-16-1623182-g001.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;BFJD promotes the clearance of persistent MRSA infection and macrophage M1 polarization in vivo . (A) Schematic illustration of a 42-day long-term persistent MRSA infection model. Mice were inoculated with 1×10 8 CFU of MRSA in 0.2 ml of PBS via a lateral tail vein. At day 28 post- MRSA inoculation, mice were administered BFJD and linezolid by oral gavage intervention, and after 14 days of treatment, the mice were euthanized for sampling. (B) Bacterial burdens in the lungs, liver, and kidneys of mice (n=6). (C) The gating strategy of macrophages (CD11b+F4/80+) in mouse lung tissue flow cytometry. (D) The percentage of macrophages in the Ly6G - cell population and relative count in total harvested cells (n=5). (E) Representative FACS plots of macrophage polarization. (F) The percentage of M1- type (CD80+CD206-) macrophages and MFI of CD80 (n=5). (G) Representative immunofluorescence micrograph of lung tissue stained with indicated antibodies for CD68, CD86 and CD206. Nuclei were revealed by DAPI staining. Scale bars, 200 μm. Data are presented as the mean ± SD. Differences were analyzed applying ordinary one-way ANOVA followed by Dunnett´s multiple comparisons test (comparison with the Infected Model). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1623182/full'>40746551</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518_sciadv.adt1318-f6.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Evaluating the repair efficacy of mitoEVs in CKD. (A) Experimental scheme of the CKD study. (B) Representative picrosirius red stained images of kidneys on day 14 after CKD (scale bar = 2 mm). (C) Quantitative analysis of the renal fibrosis area (n = 6). (D) IHC staining images for TFAM and TOM20 on day 14 after CKD (scale bar = 50 μm). (E) Quantitative analysis of TFAM and TOM20 protein expression (n = 6). (F) qPCR analysis of mitochondrial-related gene expression in the kidneys of the mice (n = 6). (G) Images of IHC staining for CD68 and ICAM on day 14 after CKD induction (scale bar = 50 μm). (H) Quantitative analysis of CD68 and ICAM protein expression (n = 6). (I) qPCR analysis of inflammation-related gene expression in the kidneys of the mice (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001, and NS = not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.science.org/doi/abs/10.1126/sciadv.adt1318'>40668934</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fphar-16-1583334-g004.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;AMPs improves APAP-induced liver inflammation in mice. (A) Immunohistochemical staining results of CD68 in mouse liver tissue. (B) Statistical results of CD68-positive area in mouse liver tissue. (C–F) The IL-1β mRNA, Nlrp3 mRNA, IL-6 mRNA and TNF-α mRNA expression levels in mouse liver tissue (n = 5). (G) Protein expression level of IL-1β in mouse liver tissue. (H) Fold change of IL-1β protein. Data of biochemical indexes were presented as mean ± SD (n = 5). # represents compared with CON group; ## p < 0.01, ### p < 0.001; * indicates compared with APAP group; * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1583334/full'>40612747</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fnut-12-1562509-g005.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/nutrition/articles/10.3389/fnut.2025.1562509/full'>40626231</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-12-758052-g005.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Immunofluorescence staining for CD68 + -Gal-9 + and CD68 + -Tim-3 + macrophages in the liver tissues of Pb ANKA-infected mice with or without α-lactose treatment. Double immunofluorescence showed expression of Gal-9 or Tim-3 on CD68 + macrophages. (A) CD68 + -Gal-9 + macrophages in the liver tissues of malarial mice and malarial mice with α-lactose treatment on day 7 p.i. (B) CD68 + -Tim-3 + macrophages in the liver tissues of malarial mice and malarial mice with α-lactose treatment on day 7 p.i. in comparison of naive and α-lactose-control. Positive cells were indicated with white arrow heads. Original magnification × 1,000.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.758052/full'>34899708</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-12974_2021_2248_fig7_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;TUDCA treatment promotes microglia polarization toward the M2 phenotype. A , C Immunofluorescent staining of Iba-1(red) /CD163(green) or CD68(red)/CD163(green) in the lesion site of the spinal cord 14 days after SCI. B , D Quantification the number of Iba-1 + /CD163 + or CD68 + /CD163 − cells in spinal cord. All experiments were performed in triplicated and data were presented as means ± SD, n =3 per group. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-021-02248-2'>34544428</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41598_2022_10973_fig7_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Inhibition of the PI3K–AKT pathway attenuates the phagocytic activation of microglia induced by sTREM-1. (A) The experimental plan was visualized. (B) The mRNA expression levels of Dlg4 , SYN1, and Homer1 in mouse hippocampus detected by Q-PCR, n = 4. (C) Representative western blot images of Dlg4, SYN1, and Homer1 protein expression in mouse hippocampus. (D) Quantitative analysis of PSD95, SYN1, and Homer1 protein level in mouse hippocampus, n = 4. (E) Representative image of IBA-1 and CD68 immunofluorescence double staining of the hippocampus in per group mice, scale bar is 200 μm. (F) Quantitative analysis of IBA-1 and CD68 double-positive cells, n = 6. (G) Representative histograms of BV2 cells phagocytosis of latex beads in different treatment groups detected by flow cytometry. (H) Phagocytosis in different BV2 cells groups detected by flow cytometry. (I) Representative western blot images of SYN1 and Homer1 protein expression in different HT22 cells groups. (J) Quantitative analysis of SYN1 and Homer1 protein level in different HT22 cells groups. Data are presented as means ± SEM of at least three separate experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-022-10973-8'>35487953</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fendo-16-1528768-g004.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Effects of 1,25 VD3 supplementation on macrophage polarization and PPARγ expression in NASH rats. (A) M1-type KCs identified by double immunofluorescence staining; green arrows indicate CD68-positive cells, red arrows indicate CD11c-positive cells, and yellow arrows indicate CD11c/CD68 double-positive cells (M1KCs). (B) M2-type KCs identified by double immunofluorescence staining; green arrows indicate CD68-positive cells, red arrows indicate CD163-positive cells, and yellow arrows indicate CD163/CD68 double-positive cells (M2KCs). Antibodies were diluted as follows: anti-CD68 (1:100), anti-CD11c (1:100), and anti-CD163 (1:200). (C) The M1/M2 macrophage ratio was determined by quantifying the proportion of M1 and M2 KCs in liver tissue, as identified through double immunofluorescence staining. (D) PPARγ levels in liver tissue of the indicated groups were determined by qPCR and expressed as relative mRNA levels following normalization to GAPDH mRNA levels. Data are presented as mean ± SEM, n=6. *p < 0.05 and ***p < 0.001 vs CG; ##p < 0.01 and ###p < 0.001 vs CDG.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2025.1528768/full'>40190400</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-oncotarget-08-106790-g008.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;miR-126-3p agomir promoted reendothelialization in vivo . ( A ) Representative Evans Blue staining photomicrographs of vessel wall harvested from normal and vein arterialization rats at 14 days after surgery. ( B ) Immunofluorescence staining for vascular endothelial cell marker CD34 (red) was used to assess endothelial recovery of the vein graft for each group. Nuclei were stained with DAPI (blue). Scale bars represent 200 μm. ( C ) Representative staining of CD68 within the intima layer 28 days after surgery is shown. Scale bars represent 50 μm. ( D ) The ratio of non-stained area (white) to the total en face area of the vein graft was used to evaluate reendothelialization. ( E ) Quantification of the reendothelialized areas was performed as a percentage of the CD34-positive surface to the total luminal surface ( F ) Quantitative evaluation of the number of accumulating CD68-positive monocytes/macrophages within the vascular wall. Values are expressed as the mean ± SEM. n =6 per group. * P < 0.05, ** P < 0.01vs NC agomir and vein graft groups.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5739774/&orig_host=www.ncbi.nlm.nih.gov'>29290989</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fphar-13-913408-g005.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Immunohistochemistry staining of wound tissues on days 7 and 14. (A) Representative images for CD31, PCNA, α-SMA, and CD68 staining (scale bar = 100 μm). (B–E) Quantification of CD31, PCNA, α-SMA, and CD68 protein expressions, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001 vs . control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs . CAH group as statistically significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9243309/&orig_host=www.ncbi.nlm.nih.gov'>35784748</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-14-1289795-g001.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;MVP positively correlates with CD68 + /Arg-1 + /CD206 + TAMs in HCC samples. (A) The histogram of Rho between MVP expression and 22 types of immune cell infiltration levels in LIHC from the TIMER2.0 database. The red box highlights the immune cells with the highest positive correlation. (B) The scatter plot of the correlation between MVP expression level with tumor purity and M2 macrophage infiltration level determined by the TIMER2.0. TPM: Transcripts Per Kilobase of exon model per Million mapped reads. (C–E) Immunofluorescence representative images and co-localization quantitative analysis of MVP (green) with CD68 (red) (C) , MVP (green) with Arg-1 (red) (D) , and MVP (green) with CD206 (red) (E) in adjacent nontumorous tissues (ANT) and HCC tumor tissues (n=10). Scale bar, 100 µm (MVP/CD68/Arg-1/CD206/Merge) and 20 µm (Zoom in). All levels of co-localization are indicated by Pearson’s correlation coefficients calculated using Image J. Data are expressed as means ± SEM, two-tailed Student’s t-test, (***P < 0.001, **P < 0.01, *P < 0.05, ns, no significance). See also and .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fimmu.2023.1289795/full'>38264642</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41598_2020_75414_fig2_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Obesity-induced pathological changes in lungs. ( a–d ) Oil Red O staining of lung in RCD and HFD mice. Less fats accumulated in the lung tissue (arrows pointing) in RCD mice ( a ), RCD + LPS mice ( c ). A large quantity of lipid droplets (arrows pointing) were accumulated in lung tissue in HFD mice ( b ) and less fat deposits in HFD + LPS mice ( d ). ( a – d ) scale bars represent 20 μm, the nuclei were re-stained blue with hematoxylin. ( e–h ) HE staining of lungs in RCD and HFD mice. HE staining of RCD mice 24 h after intratracheal administration of saline ( e ). HE staining of HFD mice 24 h after intratracheal administration of saline ( f ). HE staining of RCD mice 24 h after intratracheal administration of LPS ( g ). HE staining of HFD mice 24 h after intratracheal administration of LPS, arrows designate the alveolar inflammatory infiltration and alveolar wall thickening after LPS challenge ( h ). ( e–h ) scale bars represent 20 μm. ( i–l ) Lung immunofluorescence staining of CD68 + macrophages in RCD and HFD mice. CD68 + macrophages were stained red (arrow pointing). Lipid droplets were stained green (arrow head pointing) by Bodipy. Representative image of CD68 + macrophages observed in HFD ( j ) mice, the merged images showed the lipid droplets were located in the macrophages of the HFD mice ( j ) and the lipid droplets decreased in macrophages of HFD + LPS ( l ) mice. Representative images of CD68 + macrophages observed in RCD ( i ) and RCD + LPS ( k ) mice, the merged images indicated there were less lipid droplets in macrophages. ( i–l ) scale bars represent 10 μm. ( m–p ) Lung immunofluorescence staining of F4/80 + macrophages in RCD and HFD mice. F4/80 + macrophages were stained red (arrow pointing). Lipid droplets were stained green (arrow head pointing) by Bodipy. Representative image of F4/80 + macrophages were observed in HFD ( n ) mice, the merged images showed the lipid droplets located in the F4/80 + macrophages in HFD ( n ) mice and the lipid droplets decreased in macrophages of HFD + LPS ( p ) mice. Representative images of F4/80 + macrophages were observed in RCD ( m ), RCD + LPS ( o ) mice, the merged images indicated there were less lipid droplets in F4/80 + macrophages. ( m–p) scale bars represent 10 μm. ( q–r ) Quantification analysis of lipid accumulation in each group (n = 240 cells/12 slices/4 mice for each group). Data were normalized to the averaged signal intensity from RCD mice for each group. ( q ) Lipid intensity in CD68 + macrophages, RCD: 1.0 ± 0.09; HFD: 2.44 ± 0.27; RCD + LPS: 0.97 ± 0.16; HFD + LPS: 1.57 ± 0.19. ( r ) Lipid intensity in F4/80 + macrophages, RCD: 1.0 ± 0.13; HFD: 2.17 ± 0.31; RCD + LPS: 0.95 ± 0.18; HFD + LPS: 1.27 ± 0.19. *** P < 0.001, significant differences between each group (ANOVA, LSD-t post hoc). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-020-75414-w'>33110180</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41598_2025_86810_fig3_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Inflammation in the liver after long exposure to NMPs. ( A ) Representative images of IHC for CD68 (left panel) and semi-quantification of CD68 based on IHC staining (right panel) (n = 8). The mRNA levels of Il-1 ( B ), Il-6 ( C ), Tnf-a ( D ), Cxcl2 ( E ), Cxcl12 ( F ), and Areg ( G ) were analyzed by qPCR and normalized by β-actin (n = 6). ( H ) The protein level of IL-1β was performed by western blotting and normalized by β-actin (n = 5). ( I ) The protein level of IL-6 was performed by western blotting and normalized by β-actin (n = 5). Scale bar, 100 µm for × 200 and 50 µm for × 400. * p < 0.05, ** p < 0.01, *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-86810-5'>39833454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-15-1489679-g004.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;miR-27a-3p promotes the M2 polarization of macrophage. (A) Representative images for the immunohistochemical staining of macrophage maker CD68 and CD206 in nude mice xenografts, bar = 100 μm; and the number of CD68, CD206 positive cells per 200 × field for each group (mean ± SEM). **P < 0.01, ***P < 0.001 vs control. Below each group of images is an image showing the magnification of the corresponding box area above. (B) The expression of miR-27a-3p in mimic-miR-27a-3p or anti-miR-27a-3p treated THP1 cells was evaluated by qRT-PCR 24 h after transfection, normalized to U6 snRNA (mean ± SEM). **P < 0.01, ****P < 0.0001 vs. control. (C, D) The expression of CD206 was evaluated in mimic-miR-27a-3p or anti-miR-27a-3p treated THP1 cells by western blotting normalized to β-actin (mean ± SEM). *P < 0.05, ****P < 0.0001 vs. control. The data were from three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1489679/full'>39742261</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-ajtr0013-1290-f6.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;SJMHE1 induced M2 polarization of macrophages. A, B. Images of double immunofluorescence staining of CD206 + (green)/CD68 + (red) and CCR7 + (green)/CD68 + (red) macrophages in cross-sections of the regenerated nerve region at the midpoint of the nerve conduit. Scale bar (left) = 500 μm. The round and boxed areas are higher-magnification images. Scale bar = 200 μm and 20 μm. DAPI (blue)-stained nuclei. C, D. Quantitative analysis of the number of CD206 + /CD68 + and CCR7 + /CD68 + macrophages in different groups. The ratio of CD206 + cells to CD68 + cells was calculated as the ratio of M2 macrophages. The ratio of CCR7 + cells to CD68 + cells was calculated as the ratio of M1 macrophages. The SJMHE1 group vs the control group; n = 6 for each group. E. Immunofluorescence staining of macrophages with the phenotypic markers iNOS and Arg1. DAPI (blue)-stained nuclei. Scale bar = 100 μm. n = 3. F. Western blot analysis of iNOS and Arg1 expression in different groups. The LPS group represents the positive control group. G. Statistical analysis of the relative expression of iNOS and Arg1 proteins. The SJMHE1 group vs the control group or LPS group; n = 3 for each group; * P <0.05, ** P <0.01, *** P <0.001 (ANOVA). The data are presented as the mean ± SEM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014408/'>33841657</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41598_2024_76563_fig4_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Effects of co-culture of trophoblasts and macrophages treated with hypoxia on cellular functions. A. THP-1-derived macrophages (lower chamber) were co-cultured with HTR-8/Svneo cells (upper chamber). B. The levels of IL-1α, IL-12, and TNF-α in cell supernatants were detected by ELISA. C. Identification of the macrophage phenotype by flow cytometry. D. Identification of the macrophage phenotype by western blot analysis of phenotype markers: iNOS and CD68 for the M1-phenotype; Arg1 and CD206 for the M2-phenotype. Furthermore, THP-1-derived macrophages were treated with the supernatants of hypoxia-pretreated or non-treated HTR-8/Svneo cells E. The levels of IL-1α, IL-12, and TNF-α in cell supernatants were detected by ELISA. F. Relative expression of miR-141-3p in macrophages. G . Identification of the phenotypes of macrophages by flow cytometry. H. Identification of the phenotypes of macrophages by western blot analysis: iNOS and CD68 for the M1-phenotype; Arg1 and CD206 for the M2-phenotype. HTR-8/Svneo: HTR-8/Svneo cells cultured alone; THP-1: THP-1-derived macrophages cultured alone; HTR-8/Svneo + THP-1: HTR-8/Svneo cells co-cultured with THP-1-derived macrophages; Hyo-HTR-8/Svneo + THP-1: hypoxia-treated HTR-8/Svneo cells co-cultured with THP-1-derived macrophages; HTR-8/Svneo supernatant + THP-1: THP-1-derived macrophages treated with supernatant from HTR-8/Svneo cells; Hypo-HTR-8/Svneo supernatant + THP-1: THP-1-derived macrophages treated with supernatant from hypoxia-treated HTR-8/Svneo cells. ns: no significance; * p < 0.05, *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-76563-y'>39424901</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-12967_2020_2602_fig5_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Histological analysis. a Representative images of en face analysis. n = 6. b Quantitative analysis of lesion areas in whole aortas. Differences were assessed by unpaired students’ t -test. c Representative images of Oil Red O staining of aortic root sections. d Quantitative analysis of lesion areas in aortic root sections. e Representative images of macrophage (CD68) analysis ( b ) Quantitative analysis of lesions area in macrophage analysis. f Representative images of SMC (SMA) analysis ( g ) Quantitative analysis of lesions area in SMC. Differences were assessed by unpaired students’ t -test. * p < 0.05 vs Vehicle, ** p < 0.01 vs Vehicle. # p < 0.05 vs clopidogrel. Scale bar = 250 μm. n = 3. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-020-02602-7'>33187537</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-10020_2023_676_fig6_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Effect of Sirt6 on the inflammation of BV2 cells treated with OA&PA for 24 h. A Expression of M1-type microglia surface markers ( Cd16, Cd86 ) and M2-type microglia surface markers ( Ym1/2 ) in BV2 cells while Sirt6 was knocked down. B Expression of M1-type microglia surface markers ( Cd16, Cd86 ) and M2-type microglia surface markers ( Ym1/2 ) in BV2 cells while Sirt6 was over-expressed. C mRNA levels of Tnf-α , Il-6 , and Il-1β in BV2 cells while Sirt6 was over-expressed. D Inflammatory cytokine (TNF-α, IL-6, and IL-1β) content in culture medium while Sirt6 was over-expressed. E, F Immunofluorescence images of CD68 and TNF-α while Sirt6 was over-expressed. Scale bars: 10 μm. G, H Quantitative analysis of immunofluorescent intensity of CD68 and TNF-α while Sirt6 was over-expressed. A-D, G-H Two-way ANOVA analysis was performed, followed by the Tukey post hoc test. n = 4–8/group. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-023-00676-9'>37582706</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-ajtr0010-1817-f2.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;P210-Ab reduced plasma levels of hsCRP, MCP-1, TNF-α, and IL-6, and affected the expression of FcR (CD64) and CR1 (CD35) on PBMM. A. The titer of antibodies against P210 changes over time in ApoE -/- mice. The titer of P210-Ab started to increase significantly after the first injection and continued to increase gradually with the following booster immunization in the P210 and P210-Ab groups but increased slowly in the BSA and F-adjuvant groups. B. P210-Ab affected the plasma levels of hsCRP, MCP-1, TNF-α, and IL-6 in ApoE -/- mice at the age of 23 weeks. The plasma levels of hsCRP, MCP-1, TNF-α, and IL-6 were determined by ELISA. C. Immunization had an impact on PBMM of ApoE -/- mice. The number of CD68 + CD64 + and CD68 + CD35 + cells in P210-Ab group and BSA group (control group), respectively, are shown. Data are expressed as mean ± SEM. *P<0.05 versus BSA group; #P<0.05 versus F-adjuvant group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6038070/&orig_host=www.ncbi.nlm.nih.gov'>30018722</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-15-1363278-g001.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Transient changes in cardiac function are accompanied by changes in the number of cardiac macrophages. (A) Schematic diagram of animal modeling and experimental procedures. (B) Comparisons on Heart (g) to Tibia (cm) ratio a and Lung (g) to Heart (g) ratio b (Sham, n=12; D7, n=11; D35, n=12). Data were pooled from three independent experiments. (C) Representative images of echocardiography in cardiac function. M-mode, blood flow doppler and tissue doppler images views. (Sham, n=15; D7, n=16; D35, n=16). Data were pooled from three independent experiments. (D) Echocardiographic analysis of systolic function by M-mode: interventricular septum in diastole (IVSd b ), interventricular septum in systole (IVSs b ) and left ventricular (LV) Mass (corrected) a . (E) Echocardiographic analysis of diastolic function by blood flow doppler and tissue doppler: the speed E peak a (left ventricular early-diastolic fast filling) and the speed A peak a (left ventricular late-diastolic filling). (F) Representative images of immunofluorescent staining: CD68+ macrophages (Red), CTNT+ cardiomyocytes (Gray), DAPI+ nucleus (Blue). The yellow arrows point to macrophages. Scale bar, 50 μm. (n=4 in each group). (G) The statistical plot a of panel (F) . (H) Flow cytometry gating scheme used to identify cardiac immune cells. (I) Comparison on number of immune cells in per mg heart tissue by flow analysis. Ly6C high Monocytes c ; Neutrophils c ; the other cell types a . (J) Comparison on percentage of each cell type by flow analysis. Immune cells/All live cells (%) c ; Ly6C high Monocytes/Myeloid cells (%) c ; Neutrophils/Myeloid cells (%) b ; the other parameters a . Results of (I, J) were from three independent flow analyses. (Sham: n=12; D7: n=11; D35: n=12). “a”: Ordinary one-way ANOVA test; “b”: Kruskal-Wallis test; “c”: Welch ANOVA test. * P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1363278/full'>38601160</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-oncotarget-08-30100-g001.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Effects of macrophage depletion with clodronate liposomes on transplantation-induced arterial remodeling and inflammation. ( A ) Time course of early adventitial macrophage infiltration and subsequent neointimal hyperplasia revealed by hematoxylin and eosin (H&E) staining and immunohistochemistry. Macrophages were stained with anti-CD68; T cells stained with anti-CD3; and proliferating cells stained with anti-PCNA. ( B ) Histology images showing that macrophage depletion inhibited the growth of neointima, diminished infiltrating macrophages in the adventitia, reduced the number of PCNA + cells, and reduced inflammatory responses (samples of day 14). ( C ) Effects of clodronate on the expression levels of various inflammatory molecules measured with qPCR. ( D ) Proportion of smooth muscle cells (stained with anti-a-SM actin, indicated by arrows) and non-muscle cells (arrowheads) in the neointima of untreated and clodronate-treated vessels. Data are mean ± SD. * P < 0.05 versus control, unpaired t-test , n = 6. Dashed lines indicate the border of adventitia and media; the solid lines indicate the border between media and neointima. Bar represents 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5444729/&orig_host=www.ncbi.nlm.nih.gov'>28415796</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-10020_2023_676_fig8_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;NRF2 antagonists counteract the ameliorating effects of Sirt6 on inflammation and oxidative stress. A Immunofluorescence images of Nfκb-p65, CD68, and TNF-α in BV2 cells with combined Sirt6 overexpression and NRF2 antagonist treatment. Scale bars: 10 μm. B Quantitative analysis of immunofluorescent intensity of Nfκb-p65, CD68, and TNF-α in BV2 cells with combined Sirt6 overexpression and NRF2 antagonist treatment. n = 4/group. C Expression of inflammatory cytokines TNF-α and IL-6. n = 6–7/group. D mRNA expression of Tnf-α , Il-6 , Il-1β , and Cox-2 . n = 9–12/group. E Immunofluorescence images of ROS. Scale bars: 100 μm. F mRNA expression levels of Gclm and Sod2 . n = 9/group. B-D, F Two-way ANOVA analysis followed by the Tukey post hoc test were performed. ns: not significant. Data are presented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-023-00676-9'>37582706</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41598_2022_10973_fig3_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;sTREM-1 promotes the activation of microglia in the hippocampus of mice. (A) The gating strategy of resting and activating microglia in the mouse hippocampus. (B) Quantitative analysis of the proportion of resting microglia in the hippocampus of per group mice, n = 5. (C) Quantitative analysis of the proportion of activated microglia in the hippocampus of each group mice, n = 4. (D) Representative image of IBA-1 and CD68 immunofluorescence double staining of the hippocampus in per group mice, where the white arrows indicated CD68 + IBA-1 + cells, scale bar is 200 μm. (E) Quantitative analysis of IBA-1 + , IBA-1 and CD68 double-positive cells, n = 6; Data are presented as means ± SEM of at least three separate experiments, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-022-10973-8'>35487953</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-14-1121549-g002.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;(A) ELISA determination of IL-1β levels in the serum of young and aged SD rats. (B) Local IL-1β gene expression in young and aged SD rats. (C) Immunohistochemical detection of IL-1β secretion in Bio-Oss® and its surrounding tissues on days 7 and 14 to assess differences in the local inflammatory microenvironment of defects in young and aged rats; Red square: positive cells. (D) CD68 (red) and cl-caspase-1 (green) co-immunolabeling to evaluate NLRP3 inflammasome expression in macrophages; nuclei were stained with DAPI; White square: double-positive cells. (E, G) CD68 (red), i-NOS (green), CD68 (red) and CD206 (green) co-immunolabeling was used to evaluate macrophage phenotypes; nuclei were stained with DAPI; White square: double-positive cells. (F, H) TNF-α and IL-10 were used to assess changes in the local inflammatory microenvironment; Red square: positive cells. Aged rats showed a significant increase in M1 macrophages and a significant decrease in M2 macrophages in the local microenvironment at the sites of bone defects, suggesting a delayed local inflammatory phase, elevated systemic and local IL-1β expression, and increased NLRP3 inflammasome activation in macrophages in aged rats. *P<0.05, **P<0.01, ***P<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10157059/&orig_host=www.ncbi.nlm.nih.gov'>37153554</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-12-758157-g002.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;IL-6 neutralization prevents atrial inflammation and the alterations in Ca 2+ handling proteins in SP rats. (A, B) Representative images of atrial MPO staining (A) and quantification (B) in the 4 indicated groups. Five visual fields are taken in each sample, and the number of immune cells from these fields is quantified with ImagePro 6.0 software and is averaged to make a statistical analysis. n = 6-7/group. Scale bar: 50 µm. (C, D) Representative images of atrial CD68 staining (C) and quantification (D) . n = 6-7/group. Scale bar: 50 µm. (E–I) Original Western blot (E) and quantification of the expression of RyR2, p-RyR2 (Ser2808), p-RyR2 (Ser2814) (F) , the expression of SERCA and NCX (G) , SERCA activity (H) , the expression of PLB, p-PLB (Ser16), and p-PLB (Thr17) (I) , in atrial tissue of 4 indicated groups. n =6-8/group. * P < 0.05, *** P < 0.001 vs . Sham; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . IgG, determined by Student t-test or one-way ANOVA with Bonferroni’s post-hoc test. (F–H) .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.758157/full'>34975847</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-14-1121549-g004.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Local immunomodulation by IL-4 (A) H&E and (B) Masson’s trichrome staining. (C, D) CD68 (red), i-NOS (green), CD68 (red), and CD206 (green) co-immunolabeling was used to evaluate macrophage phenotypes; nuclei were stained with DAPI. (E, F) TNF-α and IL-10 were used to assess changes in the local inflammatory microenvironment. IL-4 recruited more macrophages at the sites of bone defects in aged rats and altered the M1/M2 ratio to form a promoted healing microenvironment by promoting the polarization of M1-type macrophages to M2-type macrophages. *P<0.05, **P<0.01, ***P<0.001. ns, no significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10157059/&orig_host=www.ncbi.nlm.nih.gov'>37153554</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-12-758052-g004.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Immunohistochemical staining for CD68 + macrophages in the livers of Pb ANKA-infected mice with or without α-lactose treatment. (A) Immunohistochemical staining of CD68 + macrophages in the livers of uninfected mice, uninfected mice with α-lactose treatment, malarial mice, and malarial mice with α-lactose treatment on day 7 p.i. Positive cells were indicated with red arrow heads. Original magnification × 1,000. (B) Morphometric analysis of liver tissues. Shown are CD68 + macrophages per square millimeter. Data are presented as means ± SD; experiments were performed with three mice per group. ### P < 0.001, malarial mice on day 5 p.i., malarial mice with α-lactose treatment on day 5 p.i., malarial mice on day 7 p.i., and malarial mice with α-lactose treatment on day 7 p.i. vs . naive group; *** P < 0.001, malarial mice with α-lactose treatment on day 5 p.i. vs . malarial mice on day 5 p.i.; *** P < 0.001, malarial mice with α-lactose treatment on day 7 p.i. vs . malarial mice on day 7 p.i.; ξ P < 0.05, malarial mice with α-lactose treatment on day 7 p.i. vs . malarial mice with α-lactose treatment on day 5 p.i.; ξξ P < 0.01, malarial mice on day 7 p.i. vs . malarial mice on day 5 p.i.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.758052/full'>34899708</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fimmu-14-1121549-g005.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Local immunomodulation by IL-4 (A) ELISA detection of IL-1β in the serum of aged SD rats. (B) Changes in local IL-1β gene expression in aged SD rats. (C) Immunohistochemical detection of IL-1β secretion in Bio-Oss® and its surrounding tissues on days 7 and 14 to assess differences in the local inflammatory microenvironment of defects in aged rats. (D) CD68 (red) and cl-caspase-1 (green) co-immunolabeling was used to evaluate NLRP3 inflammasome expression in macrophages; nuclei were stained with DAPI. (E, G) Immunohistochemical detection of BMP-2 and MMP-9 secretion in Bio-Oss® and its surrounding tissues on day 7 and day 14. (F, H) Local BMP-2 and MMP-9 gene expression in aged SD rats was used to assess the dynamic balance of local bone regeneration and bone resorption in the defects of aged rats. Local immunomodulation in aged rats resulted in reduced M1 numbers, inhibition of NLRP3 inflammasome activation, downregulation of IL-1β expression, reduced osteoclast fractionation, changes in the M1/M2 ratio, and increased BMP-2 expression, thereby promoting bone regeneration. *P<0.05, **P<0.01, ***P<0.001. ns, no significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10157059/&orig_host=www.ncbi.nlm.nih.gov'>37153554</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41419_2021_4180_fig7_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;LncRNA NLRP3/miR-138-5p/NLRP3 functions via the ceRNET during the NLRP3-triggered inflammatory response in vivo. Rat lungs were injected with PBS in the control group and LPS-treated rats were further treated with si-r-lncRNA NLRP3, Lv-lncRNA NLRP3, agomiR-138-5p, antagomiR-138-5p, Lv-lncRNA NLRP3 + agomiR-138-5p, and si-r-lncRNA NLRP3 + antagomiR-138-5p. A Lung tissue samples were collected 6 h after establishing LPS-induced ALI to analyse the histopathological changes (×200, ×400). The black arrow indicates neutrophil infiltration, pulmonary oedema, alveolar wall thickening, and alveolar haemorrhage. B The lung injury score was determined via H&E staining, a representative histological analysis ( n = 6 animals per group). C ELISA was used to measure the BALF albumin content. D Detection of the lung W/D ratio in rats. E MPO activity in the lung tissues of rats. F , G Immunohistochemical detection of the NLRP3 contents in rat lung tissues (×200, ×400). H The inflammatory response in NR8383 AM cells was suppressed by si-r-lncRNA NLRP3 and miR-138-5p mimics alone or in combination, as shown by the decreased number of cells colabeled with CD68 (green) and NLRP3 (red). LncRNA NLRP3 overexpression, miR-138-5p inhibition, and NLRP3 augmented the inflammatory response in LPS-induced ALI with more NLRP3 and CD68 anchored in the plasma membrane of the AM cells. The data are presented as mean ± SE ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41419_2023_5741_fig5_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Exogenous LPA enhanced inflammatory response in vitro and vivo. A Effect of si-GPAT3 or si-N.C. on TG content in KCs stimulated with LPS (1 μg/ml) for 24 h ( n = 3). B Bodipy 493/503 fluorescence visualized by fluorescence microscopy (scale bars represent 50 μm) ( n = 3). C LPA concentrations in the supernatant of KCs after LPS (100 ng/ml 12 h) stimulated and with or without si-GPAT3 ( n = 3). D The mRNA expression of IL-1β, NLRP3, IL-1α and IL-6 in KCs treated with LPA (30 μM 12 h) ( n = 3). E, F The effects of exogenous LPA (30 μM 12 h) on the protein expression of inflammatory factors IL-1β, NLRP3, COX2, IL-1α and IL-6 in KCs ( n = 3). G Experimental design of mice treated with LPA. H, I The contents of LDH, IL-6 and IL-1βin plasma of LPA treated with mice ( n = 6). J, K Representative images and quantification of HE staining (Scale bars represent 100 μm) and CD68 IHC (Scale bars represent 200 μm) after LPA treatment in liver of mice ( n = 3). Data represents mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-023-05741-z'>36964139</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-ajtr0013-1290-f4.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Chlorophosphate liposomes reduced the therapeutic effect of SJMHE1 in an in vivo model. A. Immunofluorescence staining of cross-sections of the regenerated nerve for CD68 (red) 35 days after nerve injury. n = 3. Scale bar = 50 μm. B. Quantitative analysis of the number of CD68-positive cells in different groups. C. Immunofluorescence staining for CD68 (red) in longitudinal sections of the regenerated nerve 10 days after nerve injury. CLP: chlorophosphate liposome. LP: liposome (negative control). n = 6. Scale bar = 50 μm. D. Quantitative analysis of the number of CD68-positive cells in different groups. E. Immunofluorescence staining for β-tubulin III (green) in longitudinal sections of the regenerated nerve. The length of regenerative axons from the proximal end was measured for different groups. Scale bar = 2000 μm. The boxed areas are higher-magnification images. Scale bar = 500 μm. DAPI (blue)-stained nuclei. F. Statistical analysis of the length of regenerative axons. SJMHE1+CLP vs SJMHE1+LP; n = 6 for each group; * P <0.05, ** P <0.01 (Student’s t test; ANOVA). The data are presented as the mean ± SEM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8014408/'>33841657</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-11658_2023_422_fig3_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;LIPUS strengthened the anti-inflammatory effect of BMSCs-derived EVs. A The protein expression levels of pro-inflammatory cytokines (IL-10 and NF-κB) in both C-EVs and LIPUS-EVs detected by ELISA assay ( n = 6 per group). B The protein expression of IL-10 and NF-κB in RAW264.7 cells measured by ELISA. And the mRNA level of IL-10 and NF-κB in RAW264.7 cells by qRT-PCR. ( n = 6 per group). C The protein expression of IL-10 and NF-κB in skin allografts measured by ELISA. The mRNA expression of IL-10 and IL-6 in skin allografts measured by qRT-PCR. ( n = 4 per group). D H&E-stained sections of skin allograft. Scale bar = 50 μm. Immunohistochemistry staining of the skin allograft in control group contained massive infiltrates of CD3 + , CD11b + and CD68 + cells in comparison to the C-EVs and LIPUS-EVs group. (Representative images from 3 different mice per group). Scale bar = 100 μm. Error bars represent mean ± SD. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Statistical significance assessed by unpaired two-tailed t -test. EVs, extracellular vesicles <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s11658-023-00422-3'>36717768</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fonc-11-620993-g003.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Infiltration of immune cells in bronchoalveolar lavage fluids and lung tissue. (A) Increased BALF cell count in urethane treated high-fat diet fed mice compared to urethane treated control diet fed mice (n=3 mice/group). **P < 0.01. (B, C) Increased expression of CD68 in lung tissue of urethane treated HFD-fed mice compared to urethane treated ND-fed mice. (B) Representative images of lung sections in urethane treated mice. Brown dots indicate the CD68 positive macrophages. (Original magnification: ×200). (C) Quantification analysis of immunohistochemistry related to CD68 between two diet groups by ImageJ. (n=4 mice/ group). *P < 0.05. (D, E) Increased expression of CD206 in lung tissue of urethane treated HFD-fed mice compared to urethane treated ND-fed mice. (D) Representative images of lung sections in urethane treated mice. Brown dots indicate the CD206 positive macrophages. (Original magnification: ×200). (E) Quantification analysis of immunohistochemistry related to CD206 between two diet groups by ImageJ. (n =4-5 mice/ group). *P < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.620993/full'>33708630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-hc9-7-e0039-g005.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Sodium cholate suppresses hepatic inflammation and fibrosis in mice fed an HFHC diet. (A) Immunohistochemical staining of CD68 in the livers of the indicated mice fed the normal chow or HFHC diet for 20 weeks. n=5 per group. Scale bar, 50 μm. (B) Quantitative real-time PCR analysis of the transcript levels of genes related to inflammation ( Il-1β, Ccl2 , and Ccl5 ). n=5 per group. (C) Representative images showing PSR staining in the livers of the indicated mice fed the normal chow or HFHC diet for 20 weeks. n=5 per group. Scale bar, 50 μm. The data are presented as the mean±SD. #indicates a significant difference between the NC group and the HFHC group ( t test); *indicates a significant difference between the L-SC (Low dose-Sodium Cholate: 90 mg/kg)/(High dose-Sodium Cholate: 180 mg/kg)/ART group and the HFHC group (one-way ANOVA). ### p <0.001 versus NC mice; * p <0.05, ** p <0.01, *** p <0.001 versus mice fed by HFHC. Abbreviations: ART, atorvastatin; CCL2, C-C motif chemokine ligand 2; CCL5, C-C motif chemokine ligand 5; CD68, fatty acid translocase CD68; HFHC, high-fat and high-cholesterol; NC, normal chow; NS, no significance; PSR, picrosirius red.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9988322/&orig_host=www.ncbi.nlm.nih.gov'>36706173</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fcimb-09-00066-g004.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Regulation of NOS2 and arginase-1. NOS2, arginase-1, and CD68 were immunodetected in colonic tissues from C57BL/6 mice ± DSS or C. rodentium ± Arg 0 , Arg NL , or Arg HIGH diets. In each panel, CD68 is depicted in red, NOS2 (A) or arginase-1 (B) in green, and the nuclei in blue; CD68 + NOS2 + and CD68 + ARG1 + cells are shown in yellow. The data shown are representative photomicrographs of at least 3 animals per condition. Scale bar, 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2019.00066/full'>30972302</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fendo-13-900392-g003.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Constant light exposure promotes renal inflammation in HFD-fed rats. (A–C) Renal mRNA expression of TNF-α , IL-6 and IL-1β in rats. (D) IHC staining of CD68 and quantification of CD68 + macrophages. Values represent mean ± SEM (n=6 for A–C , n=8 for D ). Differences were determined using a two-way ANOVA followed by a Bonferroni post hoc analysis. # p <0.05, ## p <0.01, ### p <0.001, vs. ND counterpart. * p <0.05, ** p <0.01, vs. LD counterpart. p (diet), main effect of diet; p (light), main effect of light; p (light × diet), interaction effect of light and diet.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9372432/&orig_host=www.ncbi.nlm.nih.gov'>35966094</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41419_2019_1969_fig6_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;The comparison of pancreatic injury and inflammation between Gpihbp1−/− mice with different HTG severity in caerulein-induced acute pancreatitis. a Triglyceride concentration of plasma before caerulein treatment between wild-type, HTG1 (triglyceride concentration, 1000–2000 mg/dL) and HTG2 mice(triglyceride concentration, å 2000 mg/dL). b , c Plasma amylase and lipase activity at 12 th hour after the first injection of caerulein (50 μg/kg, 10 times, hourly) between these three groups. d Representative photomicrographs of H&E-stained section of pancreas in wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. Scale bar = 500 or 100 μm. e Pathological scores of the pancreas in wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. f Incidence rates of pancreatic necrosis between these three groups after acute pancreatitis induction. Each value was the mean ± SEM for n = 6. g Immunohistochemistry evaluation for myeloperoxidase and CD68 in pancreas between wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. Scale bar = 100 μm. i Semiquantitative results of the area ratio of myeloperoxidase and CD68-positive cells among wild-type, HTG1 and HTG2 mice after acute pancreatitis induction. Each value was the mean ± SEM for n = 3–5. * p < 0.05 or ** p < 0.01 or *** p < 0.001 vs wild-type group. # p < 0.05 or ## p < 0.01 or ### p < 0.001 vs HTG1 group. WT wild-type, TG triglyceride, Ed edema, Necr necrosis, Vac vacuolisation, Infl inflammation, FFAs free fatty acids, MPO myeloperoxidase <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-019-1969-3'>31570698</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-13020_2022_639_fig8_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;BSHX decoction promoted microglia polarization from M1 to M2. A Immunofluorescent staining of CD68 (red)/CD163 (green) in the lesion site of the spinal cord 14 days after SCI. B Immunofluorescent staining of Iba-1 (red)/CD163 (green) in the lesion site of the spinal cord 14 days after SCI. C , D Quantification the number of M1 (CD68 + /CD163 − ) or M2 (Iba-1 + /CD163 + ) cells in spinal cord. All experiments were performed in triplicated and data were presented as means ± SD, n = 3 per group. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-022-00639-y'>35820953</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-ajtr0010-1817-f4.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;P210-Ab reduced atherosclerotic lesion and affected macrophages and the content of collagen in the aortic sinus. (A, B) The inhibitory effect of P210-Ab on the atherosclerosis of ApoE -/- mice at the age of 23 weeks. Representative images of en face (A) and aortic sinus sections (B) by Oil-red-O staining. Quantitative analyses of en face and aortic sinus sections (100 ×) located in the right column. (C, D) Representative images of aortic sinus section after immunohistochemistry for CD68 (C) (100 ×) and Masson staining (D) (100 ×). Quantitative analyses of CD68 and collagen in the aortic sinus. Data are expressed as mean ± SEM; n = 5, 8, or 9; * P <0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6038070/&orig_host=www.ncbi.nlm.nih.gov'>30018722</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-fendo-13-864703-g004.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Effects of FTZ on metabolic inflammation and fibrosis in the livers of mice. (A) Representative images showing PSR and immunohistochemical staining of CD68 staining in the livers of the indicated mice (n=6). (B) Relative mRNA levels of inflammatory genes in the livers of the indicated mice (n=7–8). (C) Relative mRNA levels of profibrotic genes in the livers of the indicated mice (n=7-8). (D) The expression of CD68, TLR-4, and β-actin was analyzed by Western blotting. β-actin served as a loading control (n=3). (E) The expression of α-SMA, p-Smad3, Smad2/3, and β-actin was analyzed by Western blotting. β-actin served as a loading control (n=3). Data are represented as means ± SEM. # indicates a significant difference between the NCD group and the HFD group (t-test); * indicates a significant difference between the FTZ (600 mg/kg)/FTZ (1,200mg/kg)/ATV (10 mg/kg) group and the HFD group (one-way ANOVA). ## P < 0.01 versus NCD mice; * P < 0.05, ** P < 0.01 versus mice fed by HFD. ns indicates no significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9243428/&orig_host=www.ncbi.nlm.nih.gov'>35784533</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-oncotarget-08-83872-g009.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;KX2-391 inhibits peritoneal macrophage infiltration in the injured peritoneum. Peritoneal membrane was collected at 21 days after CG injury with or without administration of KX2-391(KX2) ( A , B ). (A) Photomicrographs illustrate immunohistochemical staining of CD68 in the submesothelial compact zone. (B) The number of CD68-positive cells (yellow) was calculated from 10 random fields (200 X) of six rat peritoneal samples. Data are means ± S.E.M. ( n = 6). Bars with different letters (a–c) are significantly different from one another ( P < 0.05).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5663561/&orig_host=www.ncbi.nlm.nih.gov'>29137389</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-41413_2021_172_fig8_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;Mice lacking ProCT display increased numbers of macrophages in the bone marrow after iPTH treatment. a Ccl2 expression levels (fold differences) in the indicated tissues following 4 weeks of iPTH treatment (100 μg·kg −1 ). BM = whole bone marrow. n = 3-4 mice per group (one-way ANOVA followed by Tukey’s post hoc test). b Flow cytometric analyses and ( c ) representative flow cytometry plots of the indicated cell populations in flushed bone marrow from mice of the indicated genotypes following 4 weeks of iPTH (100 μg·kg −1 ) and/or ProCT (10 μg·kg −1 ) treatment. Please also refer to Supplementary Fig. for the gating strategy. n = 5–9 mice per group. For Calca −/− mice, the data were pooled from two independent experiments. d Representative images of immunohistochemical staining of tibia sections derived from the same mice using a Cd68-specific antibody. Scale bar = 100 μm. Quantification of intramedullary and endocortical Cd68-positive cells per bone marrow area is displayed on the right. n = 4-5 mice per group. In ( b – d ), one-way ANOVA followed by Tukey’s post hoc test was applied. e Schematic representation of the proposed function of Calca -derived peptides in regulating the therapeutic effect of iPTH. Left: iPTH (teriparatide) induces ProCT expression in osteoblasts, which impairs the recruitment of osteoclast precursors (i.e., monocytes and macrophages) to the bone surface and thus inhibits bone resorption. Right: Circulating CT impairs the osteoanabolic effect of iPTH based on tonic inhibition of bone formation, presumably via osteoclasts <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41413-021-00172-y'>35087025</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-13020_2022_639_fig9_html.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;BSHX decoction promoted remyelination activity. Immunofluorescent staining of activated microglia (CD68 positive, red) and oligodendrocytes (MBP positive, green) in the lesion site of the spinal cord 14 days after SCI <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-022-00639-y'>35820953</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1518-13287_2022_2980_fig7_html.pngAnti-Macrosialin CD68 Antibody Picoband&reg;PF-127/hADSCs-Exos complex treatment inhibits inflammatory reaction. a Representative images of TNF-α immunostaining at 4, 7, and 10 days after treatment. Scale bar = 20 µm. b Quantification of TNF-α + IHC stained tissues. c Representative images illustrating IHC results of IL-6 at 4, 7, and 10 days after surgery. Scale bar = 20 µm. d Quantification of IL-6 + IHC stained tissues. e IHC images of wound sections stained with CD68 on days 4, 7, and 10 post-wounding. Scale bar = 20 µm. f Quantification of the number of CD68 positive cells in the wound area on days 4, 7, and 10. g IHC images of wound sections stained with CD206 at days 4, 7, and 10 post-wounding. Scale bar = 20 µm. h Quantification of the number of CD206 positive cells in the wound area on days 4, 7, and 10. In b, d, and f , data are shown as mean ± SEM; n = 6 for each group. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 versus vehicle control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-022-02980-3'>35941707</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-camkk-antibody-pa1520-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1520-camkk1-primary-antibodies-wb-testing-1.jpgAnti-CaMKK/CAMKK1 Antibody Picoband&reg;Western blot analysis of CAMKK1 using anti-CAMKK1 antibody (PA1520). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human U87 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAMKK1 antigen affinity purified polyclonal antibody (PA1520) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAMKK1 at approximately 56 kDa. The expected band size for CAMKK1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1520-2-IHC-anti-camkk-antibody.jpgAnti-CaMKK/CAMKK1 Antibody Picoband&reg;Anti-CaMKK antibody&#44; PA1520&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1520-camkk1-primary-antibodies-ihc-testing-3.jpgAnti-CaMKK/CAMKK1 Antibody Picoband&reg;IHC analysis of CAMKK1 using anti-CAMKK1 antibody (PA1520). <br>CAMKK1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CAMKK1 Antibody (PA1520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-canstatin-antibody-pa1521-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1521-canstatin-primary-antibodies-wb-testing-1.jpgAnti-Canstatin Antibody Picoband&reg; Western blot analysis of Canstatin using anti-Canstatin antibody (PA1521). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Canstatin antigen affinity purified polyclonal antibody (Catalog # PA1521) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Canstatin at approximately 24-35 kDa. The expected band size for Canstatin is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1521-canstatin-primary-antibodies-ihc-testing-2.jpgAnti-Canstatin Antibody Picoband&reg; IHC analysis of Canstatin using anti-Canstatin antibody (PA1521). <br> Canstatin was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Canstatin Antibody (PA1521) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1521-canstatin-primary-antibodies-ihc-testing-3.jpgAnti-Canstatin Antibody Picoband&reg; IHC analysis of Canstatin using anti-Canstatin antibody (PA1521). <br> Canstatin was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Canstatin Antibody (PA1521) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1521-canstatin-primary-antibodies-ihc-testing-4.jpgAnti-Canstatin Antibody Picoband&reg; IHC analysis of Canstatin using anti-Canstatin antibody (PA1521). <br> Canstatin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Canstatin Antibody (PA1521) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-1-p10-antibody-pa1522-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1522-2-IHC-anti-caspase-1-p10-antibody.jpgAnti-Caspase-1(P10)/CASP1 Antibody Picoband&reg;Anti-Caspase-1(P10) antibody&#44; PA1522&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1522-1_1-WB-anti-caspase-1-p10-antibody.jpgAnti-Caspase-1(P10)/CASP1 Antibody Picoband&reg;Anti-Caspase-1(P10) antibody&#44; PA1522&#44; Western blotting<br>All lanes: Anti CASP1(P10) (PA1522) at 0.5ug/ml<br>Lane 1: MCF-7 Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: SW620 Whole Cell Lysate at 40ug<br>Predicted bind size: 45KD<br>Observed bind size: 45KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-8-p10-antibody-pa1524-boster.html2026-03-24T05:03:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1524-1-WB-anti-caspase-8-p10-antibody.jpgAnti-Caspase-8(P10)/CASP8 Antibody Picoband&reg;Anti-Caspase-8(P10) antibody&#44; PA1524&#44; Western blotting<br>WB: HELA Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1524-2-IHC-anti-caspase-8-p10-antibody.jpgAnti-Caspase-8(P10)/CASP8 Antibody Picoband&reg;Anti-Caspase-8(P10) antibody&#44; PA1524&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-i-309-antibody-pa1525-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1525-1-WB-anti-ccl1-i-309-antibody.jpgAnti-I-309/CCL1 Antibody Picoband&reg;Anti-I-309 antibody&#44; PA1525&#44; Western blotting<br>All lanes: Anti I-309 (PA1525) at 0.5ug/ml<br> Lane 1: SCG Whole Cell Lysate at 40ug<br> Lane 2: COLO320 Whole Cell Lysate at 40ug<br> Lane 3: JURKAT Whole Cell Lysate at 40ug<br> Predicted bind size: 11KD<br> Observed bind size: 18KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd147-antibody-pa1526-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1526-bsg-primary-antibodies-wb-testing-1.jpgAnti-CD147/BSG Antibody Picoband&reg; Western blot analysis of CD147/BSG using anti-CD147/BSG antibody (PA1526). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/BSG antigen affinity purified polyclonal antibody (Catalog # PA1526) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147/BSG at approximately 35-60 kDa. The expected band size for CD147/BSG is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1526-bsg-primary-antibodies-ihc-testing-2.jpgAnti-CD147/BSG Antibody Picoband&reg; IHC analysis of CD147/BSG using anti-CD147/BSG antibody (PA1526). <br> CD147/BSG was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD147/BSG Antibody (PA1526) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1526-bsg-primary-antibodies-ihc-testing-3.jpgAnti-CD147/BSG Antibody Picoband&reg; IHC analysis of CD147/BSG using anti-CD147/BSG antibody (PA1526). <br> CD147/BSG was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD147/BSG Antibody (PA1526) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1526-bsg-primary-antibodies-ihc-testing-4.jpgAnti-CD147/BSG Antibody Picoband&reg; IHC analysis of CD147/BSG using anti-CD147/BSG antibody (PA1526). <br> CD147/BSG was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD147/BSG Antibody (PA1526) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1526-bsg-primary-antibodies-ihc-testing-5.jpgAnti-CD147/BSG Antibody Picoband&reg; IHC analysis of CD147/BSG using anti-CD147/BSG antibody (PA1526). <br> CD147/BSG was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD147/BSG Antibody (PA1526) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1526-bsg-primary-antibodies-ihc-testing-6.jpgAnti-CD147/BSG Antibody Picoband&reg; IHC analysis of CD147/BSG using anti-CD147/BSG antibody (PA1526). <br> CD147/BSG was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD147/BSG Antibody (PA1526) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stra8-antibody-pa1528-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1528-1-WB-anti-stra8-antibody.jpgAnti-Stra8 Antibody Picoband&reg;Anti-Stra8 antibody&#44; PA1528&#44; Western blotting<br>All lanes: Anti Stra8(PA1528) at 0.5ug/ml<br>WB : Rat Testis Tissue Lysate at 50ug<br>Predicted bind size: 37KD<br>Observed bind size: 37KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-4-antibody-pa1529-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1529-1-WB-anti-il-4-antibody.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Anti-IL-4 antibody&#44; PA1529&#44; Western blotting<br>Lane 1: Recombinant Mouse IL-4 Protein 10ng<br>Lane 2: Recombinant Mouse IL-4 Protein 5ng<br>Lane 3: Recombinant Mouse IL-4 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eotaxin-antibody-pa1531-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1531-1-WB-anti-eotaxin-antibody.jpgAnti-Eotaxin/CCL11 Antibody Picoband&reg;Anti-Eotaxin antibody&#44; PA1531&#44; Western blotting<br>Lane 1: Recombinant mouse Eotaxin Protein 10ng <br>Lane 2: Recombinant mouse Eotaxin Protein 5ng<br>Lane 3: Recombinant mouse Eotaxin Protein 2.5ng<br> Lane 4: Recombinant mouse Eotaxin Protein 1.25ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-1-beta-antibody-pa1533-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1533-il1b-primary-antibodies-wb-testing-1.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;Western blot analysis of IL1B using anti-IL1B antibody (PA1533). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant rat IL1B protein 10 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1B antigen affinity purified polyclonal antibody (PA1533) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL1B at approximately 17 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tbp-antibody-pa1534-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1534-1-WB-anti-tbp-tata-binding-protein-tbp-antibody.jpgAnti-TATA binding protein TBP Antibody Picoband&reg;Anti-TBP antibody&#44; PA1534&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: MM231 Cell Lysate<br>Lane 3: MM231 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adam10-antibody-pa1535-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1535-1-WB-anti-adam10-antibody.jpgAnti-ADAM10/ADAM10 Antibody Picoband&reg;Anti-ADAM10 antibody&#44; PA1535&#44; Western blotting<br>Lane 1: Recombinant Human ADAM10 Protein 10ng<br>Lane 2: Recombinant Human ADAM10 Protein 5ng<br>Lane 3: Recombinant Human ADAM10 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1535-oncotarget-07-70092-g003.jpgAnti-ADAM10/ADAM10 Antibody Picoband&reg;Gemcitabine-mediated shedding of ULBP2 is ADAM10-dependent. A. ADAM10 expression of PANC-1 and MIA PACA-2 cells was determined when 2 μmol/l gemcitabine was added into cell culture. B. Cells were transfected with ADAM10 siRNA or control siRNA for 48 h and the expression of mRNA and protein of ADAM10 was evaluated by Q-PCR and western blot. C. sULBP2 in the culture supernatant was evaluated by ELISA. *P<0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5342537/&orig_host=www.ncbi.nlm.nih.gov'>27602753</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1535-oncotarget-07-70092-g004.jpgAnti-ADAM10/ADAM10 Antibody Picoband&reg;Immunohistochemical staining for ADAM10. The ADAM10 were principally localized in cytoplasm of tumor cells with varying staining intensity. A. High expression of ADAM10 (200x). B. High expression of ADAM10 (400x). C. Low expression of ADAM10 (200x). D. Low expression of ADAM10 (400x). E. Negative ADAM10 expression (200x). F. Partial enlargement of ADAM10 staining with the magnifying power of 400x.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5342537/&orig_host=www.ncbi.nlm.nih.gov'>27602753</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atf4-antibody-pa1537-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1537-1-WB-anti-atf4-antibody.jpgAnti-ATF4 Antibody Picoband&reg;Anti-ATF4 antibody&#44; PA1537&#44; Western blotting<br>All lanes: Anti ATF4 (PA1537) at 0.5ug/ml<br> Lane 1: A431 Whole Cell Lysate at 40ug<br> Lane 2: RAJI Whole Cell Lysate at 40ug<br> Lane 3: CEM Whole Cell Lysate at 40ug<br> Lane 4: HUT Whole Cell Lysate at 40ug<br> Predicted bind size: 39KD<br> Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcat1-antibody-pa1538-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-bcat1-primary-antibodies-wb-testing-1_1.jpgAnti-BCAT1 Antibody Picoband&reg;Western blot analysis of BCAT1 using anti-BCAT1 antibody (PA1538). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human MOLT-4 whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAT1 antigen affinity purified polyclonal antibody (Catalog # PA1538) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCAT1 at approximately 43 kDa. The expected band size for BCAT1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-bcat1-primary-antibodies-ihc-testing-1.jpgAnti-BCAT1 Antibody Picoband&reg;IHC analysis of BCAT1 using anti-BCAT1 antibody (PA1538). <br> BCAT1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (PA1538) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-bcat1-primary-antibodies-ihc-testing-2_1.jpgAnti-BCAT1 Antibody Picoband&reg;IHC analysis of BCAT1 using anti-BCAT1 antibody (PA1538). <br> BCAT1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCAT1 Antibody (PA1538) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-bcat1-primary-antibodies-fcm-testing-1.jpgAnti-BCAT1 Antibody Picoband&reg;Flow Cytometry analysis of Jurkat cells using anti-BCAT1 antibody (PA1538). <br> Overlay histogram showing Jurkat cells stained with PA1538 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BCAT1 Antibody (PA1538, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-41419_2020_2930_fig6_html.pngAnti-BCAT1 Antibody Picoband&reg;ZNF423 maintaines the stable expression of BCAT1 by binding the AU-rich elements (AREs) of the 3′-UTR of BCAT1 mRNA in hypoxic PASMCs. a The binding sites for ZNF423 in the 3′-UTR of BCAT1 mRNA. b The correlation between ZNF423 and BCAT1 mRNA was detected by real-time PCR after RNA immunoprecipitation (RIP) ( n = 3). c Reporter constructs containing luciferase, and the 3′-UTR of BCAT1 mRNA and mutated 3ʹ-UTR of BCAT1 mRNA were used to estimate the activity of various luciferase reporter genes ( n = 3). Nor normoxia, Hyp hypoxia, Con con083 control vector, 3′-UTR 3′-UTR luciferase reporter plasmid, 3′-UTR mut 3′-UTR ARE mutant luciferase reporter plasmid. Statistical analysis was performed with two-way ANOVA. All values are presented as the mean ± SEM. ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-41419_2020_2930_fig5_html.pngAnti-BCAT1 Antibody Picoband&reg;Hypoxia leads to the transfer of ZNF423 from the nucleus to the cytoplasm, where it bound BCAT1 to promote autophagy activity. a Bioinformatics analysis of proteins associated with BCAT1. Upside: According to the JASPAR database and LASAGNA-Search 2.0 database, there was 28 genes that may bind to bcat1, and the binding ability of ZNF423, STAT1, Pou5f1, STAT3, SP1, SOX9, and TEAD1 was strong. Underside: RT-PCR analysis of the mRNA levels of ZNF423, STAT1, Pou5f1, STAT3, SP1, SOX9, and TEAD1 with rat β-actin serving as the standard in PASMCs under NOR or HYP for 24 h ( n = 5). b Western blot analysis of the expression of ZNF423 in PASMCs under NOR or HYP for 24 h ( n = 6). c ZNF423 protein levels were assayed in pulmonary arterial tissues of hypoxic model rats ( n = 4). d Coimmunoprecipitation of whole-cell lysates of PASMCs exposed to normoxia or hypoxia for 24 h with anti-ZNF423, followed by probing with anti-BCAT1 ( n = 3). e Western blot analysis of BCAT1 expression in PASMCs transfected with ZNF423 siRNA under NOR or HYP for 24 h ( n = 4). f PASMCs were exposed to HYP for 24 h, and the colocalization between BCAT1 and ZNF423 was determined by immunofluorescence. GFP-BCAT1 (green), ZNF423 (red), and DAPI (blue). Scale bar = 50 μm ( n = 3). g The translocation of ZNF423 between the nucleus and cytoplasm in PASMCs transfected with BCAT1 siRNA or gabapentin ( n = 3). h Western blot analysis of the expression of BECN1 and Atg5 in PASMCs transfected with ZNF423 siRNA under HYP for 24 h ( n = 4). i Autophagic flux of PASMCs cotransfected with eGFP-mRFP-LC3 plasmid and control siRNA or ZNF423 siRNA under HYP for 24 h. Scale bar = 50 μm ( n = 5). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, H + si-ZNF423 hypoxia plus ZNF423 siRNA, IP immunoprecipitation, IB immunoblotting. Statistical analysis was performed with one-way ANOVA or the Student’s t test. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-41419_2020_2930_fig4_html.pngAnti-BCAT1 Antibody Picoband&reg;BCAT1 regulates autophagy during hypoxia by activating ERs via the IRE1-XBP1-RIDD axis. a Western blot analysis of BECN1 and Atg5 in PASMCs cotransfected with BCAT1 and IRE1 siRNA ( n = 5). b Autophagic flux was monitored in PASMCs cotransfected with eGFP-mRFP-LC3 plasmid and control siRNA or IRE1 siRNA that were then exposed to HYP for 24 h. Scale bar = 50 μm ( n = 3). c , d RT-PCR analysis of the mRNA levels of XBP1-s, sparc, pmp2, and Scara3 with rat β-actin serving as the standard ( n = 5). e The formation of autophagosomes was detected, and autophagic activity was estimated in cells in which the expression of XBP1 was knocked down with XBP1 siRNA under HYP for 24 h. Scale bar = 50 µm ( n = 5). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, H + SI-IRE1 hypoxia plus IRE1 siRNA, H + SI-XBP1 hypoxia plus XBP1 siRNA, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid, H + Con+NC hypoxia plus control vector plus control siRNA, H + B + Si-IRE hypoxia plus BCAT1 plasmid plus IRE1 siRNA. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-41419_2020_2930_fig3_html.pngAnti-BCAT1 Antibody Picoband&reg;BCAT1 regulates autophagy through the endoplasmic reticulum stress pathway. a Expression of BCAT1 and ER-Tracker Red staining in PASMCs exposed to NOR or HYP for 24 h. Scale bar = 50 μm ( n = 3). b Western blot analysis of PERK, IRE1, ATF6, and GRP78 protein expression in the ERs pathway in PASMCs treated with gabapentin ( n = 8). c Western blot analysis of IRE1, PERK, ATF6, and GRP78 expression in PASMCs transfected with BCAT1 siRNA ( n = 8). d Western blot analysis of BECN1 and Atg5 in PASMCs treated with the ERs pathway inhibitor 4-PBA and BCAT1 plasmid ( n = 8). e Coimmunoprecipitation of the whole-cell lysates of PASMCs exposed to normoxia or hypoxia for 24 h with anti-IRE1, followed by probing with anti-BCAT1 ( n = 3). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, N + Con normoxia plus control vector, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid, H + Con+4 hypoxia plus control vector plus 4-phenylbutyric acid, H + B + 4 hypoxia plus BCAT1 plasmid plus 4-phenylbutyric acid, IP immunoprecipitation, IB immunoblotting. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-41419_2020_2930_fig2_html.pngAnti-BCAT1 Antibody Picoband&reg;Upregulation of BCAT1 expression induced by hypoxia leads to PASMC autophagy. a Western blot analysis of BECN1 and Atg5 protein expression in PASMCs treated with the inhibitor gabapentin (20 µM) ( n = 8). b Western blot analysis of BECN1 and Atg5 protein expression in PASMCs transfected with BCAT1 siRNA or BCAT1 plasmid ( n = 8). c , d Immunofluorescence staining for BECN1 and Atg5 in PASMCs. BECN1 and Atg5 (green), α-SMA (red), and DAPI (blue). Scale bar = 50 μm ( n = 3). e Western blot analysis of BECN1 and Atg5 expression in the pulmonary arterial tissues of hypoxia model rats treated with gabapentin ( n = 7). f Measurement of autophagic flux in PASMCs transfected with eGFP-mRFP-LC3 plasmid and exposed under NOR or HYP for 24 h treated with BCAT1 siRNA or the BCAT1 inhibitor gabapentin. Yellow and red dots indicate autolysosomes and autophagosomes, respectively. Scale bar = 50 μm ( n = 6). Nor normoxia, Hyp hypoxia, Mct monocrotaline, H + G hypoxia plus gabapentin, M + G monocrotaline plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1538-41419_2020_2930_fig1_html.pngAnti-BCAT1 Antibody Picoband&reg;Hypoxia resultes in the increased expression of BCAT1. a Western blot analysis of BCAT1 expression in hypoxic PASMCs ( n = 8). b Subcellular distribution of BCAT1 in PASMCs determined by immunofluorescence analysis. Scale bars: 50 μm ( n = 3). c The cellular expression of BCAT1 in the smooth muscle layer of lung tissues from hypoxic model rats determined by immunofluorescence staining analysis. Scale bar = 100 μm ( n = 3). d BCAT1 protein levels in pulmonary arterial tissues of hypoxia model rats ( n = 8). e Time course of BCAT1 expression of PASMCs at 0, 6, 12, 24, 48, and 72 h after hypoxia treatment ( n = 6). Nor normoxia, Hyp hypoxia, Mct monocrotaline. Statistical analysis was performed with one-way ANOVA or the Student’s t test. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; ns not significant. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caveolin-2-antibody-pa1540-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1540-1-WB-anti-caveolin-2-antibody.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg;Anti-Caveolin-2 antibody&#44; PA1540&#44; Western blotting<br>Lane 1: Rat Heart Tissue Lysate<br>Lane 2: Rat lung Tissue Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: A431 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1540-2-WB-anti-caveolin-2-antibody.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg;Anti-Caveolin-2 antibody&#44; PA1540&#44; Western blotting<br>All lanes: Anti Caveolin-2 (PA1540) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: SMMC Whole Cell Lysate at 40ug<br> Lane 3: COLO320 Whole Cell Lysate at 40ug<br> Predicted bind size: 17KD<br> Observed bind size: 17KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1540-3-IHC-anti-caveolin-2-antibody.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg;Anti-Caveolin-2 antibody&#44; PA1540&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1540-4-IHC-anti-caveolin-2-antibody.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg;Anti-Caveolin-2 antibody&#44; PA1540&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-baff-antibody-pa1541-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1541-1-WB-anti-baff-antibody.jpgAnti-BAFF/TNFSF13B Antibody Picoband&reg;Anti-BAFF antibody&#44; PA1541&#44; Western blotting<br>Lane 1: Recombinant Human BAFF Protein 10ng<br>Lane 2: Recombinant Human BAFF Protein 5ng<br>Lane 3: Recombinant Human BAFF Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1541-2-IHC-anti-baff-antibody.jpgAnti-BAFF/TNFSF13B Antibody Picoband&reg;Anti-BAFF antibody&#44; PA1541&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk1-antibody-pa1544-boster.html2026-03-24T05:03:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1544-fcimb-07-00029-g003.jpgAnti-Cyclin-dependent kinase 1 CDK1 Antibody Picoband&reg;EV-D68 infection prevents cell exit from G0/G1 into S phase and promotes G2/M to G0/G1 transition . (A) RD cells were serum-starved for 24 h and then mock-infected (mock) or infected with EV-D68 Fermon strain (infected) at an MOI of 0.8. After 2 h of virus adsorption, the cells were treated with medium without FBS for 18 h, followed by medium containing 10% FBS. Top panel: The cell cycle profiles were determined by flow cytometry at 0, 18, 24, and 30 h post-infection. Bottom panel: The histograms show the percentage of cells in each phase of the cell cycle. (B) G0/G1 and S phase-related cell cycle proteins were analyzed by western blot analysis. RD cells were mock-infected (m) or infected with EV-D68 Fermon strain at an MOI of 0.8 (i) for 0, 16, 20, 24, and 28 h. Histone is shown as a loading control. Results are representative of three independent experiments. (C) At 24 h post-infection, mRNA levels of CDK2, cyclinE1, CDK4, CDK6, and cyclinD were evaluated in mock infected (mock) and EV-D68 infected (infected) cells by quantitative real-time PCR. The results are standardized to GAPDH and normalized to 1.0 in mock-infected cells. (D) RD cells were treated with 25 ng/mL nocodazole or control medium for 24 h, infected with EV-D68 Fermon strain at an MOI of 0.8 or mock for 2 h and then re-treated with 25 ng/mL nocodazole for synchronization. Top panel: Cell-cycle profiles were determined by flow cytometry. Low panel: The histograms show the percentage of cells in each phase of the cell cycle. (E) G2/M phase-related proteins were detected by western blot analysis. Results are representative of three independent experiments. (F) At 24 h post-infection, mRNA levels of CDK1 and cyclinB1 were assessed in mock-infected (mock) and EV-D68 infected (infected) cells by quantitative real-time PCR. The results are standardized to GAPDH mRNA and normalized to 1.0 in mock-infected cells. (G) At 28 h post-infection, the protein concentration of CDK4, CDK6, cyclinD1, CDK2, cyclinE1, CDK1, cyclinB1, and histone were assessed in mock-infected (mock) and EV-D68 infected (infected) cells by Elisa kit. The results are standardized to Histone (his). The results indicate the mean ± S.D of three independent experiments. * P < 0.05, ** P < 0.01, and *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2017.00029/full'>28229049</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1544-cdc2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin-dependent kinase 1 CDK1 Antibody Picoband&reg; Western blot analysis of CDK1 using anti-CDK1 antibody (PA1544). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human SH-SY5Y whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 antigen affinity purified polyclonal antibody (Catalog # PA1544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1 at approximately 34 kDa. The expected band size for CDK1 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk2-antibody-pa1547-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1547-cdk2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin-dependent kinase 2 Cdk2 Antibody Picoband&reg; Western blot analysis of CDK2 using antiCDK2 antibody (PA1547). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human U2OS whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: human T47D whole cell lysates,<br> Lane 6: human CACO-2 whole cell lysates,<br> Lane 7: human 293T whole cell lysates,<br> Lane 8: human PC-3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK2 antigen affinity purified polyclonal antibody (Catalog # PA1547) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK2 at approximately 30 kDa. The expected band size for CDK2 is at 30, 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1547-cdk2-primary-antibodies-wb-testing-2.jpgAnti-Cyclin-dependent kinase 2 Cdk2 Antibody Picoband&reg; Western blot analysis of CDK2 using antiCDK2 antibody (PA1547). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human U2OS whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat L6 whole cell lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK2 antigen affinity purified polyclonal antibody (Catalog # PA1547) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK2 at approximately 30 kDa. The expected band size for CDK2 is at 30, 34 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk5-antibody-pa1548-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1548-cdk5-primary-antibodies-wb-testing-1.jpgAnti-Cyclin-dependent-like kinase 5 Cdk5 Antibody Picoband&reg;Western blot analysis of Cdk5 using anti-Cdk5 antibody (PA1548). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk5 antigen affinity purified polyclonal antibody (PA1548) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cdk5 at approximately 33 kDa. The expected band size for Cdk5 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1548-cdk5-primary-antibodies-if-testing-1.jpgAnti-Cyclin-dependent-like kinase 5 Cdk5 Antibody Picoband&reg;IF analysis of Cdk5 using anti-Cdk5 antibody (PA1548) and anti-Alpha Tubulin antibody (M03989-3). <br>Cdk5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cdk5 Antibody (PA1548) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1548-cdk5-primary-antibodies-fcm-testing-1.jpgAnti-Cyclin-dependent-like kinase 5 Cdk5 Antibody Picoband&reg;Flow Cytometry analysis of Jurkat cells using anti-Cdk5 antibody (PA1548). <br>Overlay histogram showing Jurkat cells stained with PA1548 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cdk5 Antibody (PA1548, 1 μg/1x10⁶ cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd89-antibody-pa1549-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1549-cd89-primary-antibodies-wb-testing-1.jpgAnti-CD89/FCAR Antibody Picoband&reg; Western blot analysis of CD89/FCAR using anti-CD89/FCAR antibody (PA1549). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human CACO-2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD89/FCAR antigen affinity purified polyclonal antibody (Catalog # PA1549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody (Left) at a dilution of 1:2000 or a goat anti-rabbit IgG-HRP Conjugated secondary antibody (Right) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD89/FCAR approximately 70 kDa. The expected band size for CD89/FCAR is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gbp1-antibody-pa1550-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1550-1-WB-anti-gbp1-antibody.jpgAnti-Guanylate-binding protein 1 GBP1 Antibody Picoband&reg;Anti-GBP1 antibody&#44; PA1550&#44; Western blotting<br>All lanes: Anti GBP1 (PA1550) at 0.5ug/ml<br> Lane 1: U87 Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 68KD<br> Observed bind size: 68KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikk-gamma-antibody-pa1551-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1551-1-WB-anti-ikk-gamma-antibody.jpgAnti-IKK gamma/IKBKG Antibody Picoband&reg;Anti-IKK gamma antibody&#44; PA1551&#44; Western blotting<br>Lane 1: Mouse Liver Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br>Lane 3: Mouse Ovary Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hcg-receptor-antibody-pa1552-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1552-1-IHC-anti-hcg-receptor-antibody.jpgAnti-hCG receptor/LHCGR Antibody Picoband&reg;Anti-hCG receptor antibody&#44; PA1552&#44; IHC(P)<br>IHC(P): Rat Ovary Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1552-2-WB-anti-hcg-receptor-antibody.jpgAnti-hCG receptor/LHCGR Antibody Picoband&reg;Anti-hCG receptor antibody&#44; PA1552&#44; Western blotting<br>All lanes: Anti hCG receptor (PA1552) at 0.5ug/ml<br>WB: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 78KD<br>Observed bind size: 78KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1552-lhcgr-primary-antibodies-ihc-testing-2.jpgAnti-hCG receptor/LHCGR Antibody Picoband&reg;IHC analysis of LHCGR using anti-LHCGR antibody (PA1552). <br>LHCGR was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHCGR Antibody (PA1552) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1552-fendo-14-1153374-g001.jpgAnti-hCG receptor/LHCGR Antibody Picoband&reg;Distribution of aldosterone synthase (CYP11B2) and LHCGR in the patient’s adrenal. (A) , Distribution of CYP11B2 immunoreactivity in the tumor region (T) of the adrenal tissue. (B-E) , Distribution of CYP11B2 (B, D) and LHCGR (C, E) immunoreactivities in consecutive sections of the APA tissue at low (B, C) and high (D, E) magnifications. Similar distribution of CYP11B2 and LHCGR immunoreactivities were observed in some areas (arrows) in (B, C) . PT indicates peritumoral tissue; and V, vein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2023.1153374/full'>36926028</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1552-41598_2018_22797_fig4_html.jpgAnti-hCG receptor/LHCGR Antibody Picoband&reg;The effects of treatment on ovarian FSHR, LHR, ER, and PR expression level. ( A ) The FSHR, LHR, ER, and PR were detected by immunohistochemistry, red arrows represent the expression of receptors. ( B ) The positive rate of FSHR, LHR, ER, and PR in four groups. *Represents MPA + hMG group vs control group, MPA group or hMG group. *P < 0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-22797-6'>29535409</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctbp2-antibody-pa1554-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1554-ctbp2-primary-antibodies-wb-testing-1.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; Western blot analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates, <br> Lane 4: human U20S whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP2 antigen affinity purified polyclonal antibody (Catalog # PA1554) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTBP2 at approximately 49 kDa. The expected band size for CTBP2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1554-2-IHC-anti-ctbp2-antibody.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> CTBP2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PA1554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1554-3-IF-anti-ctbp2-antibody.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> CTBP2 was detected in immunocytochemical section of human Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PA1554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1554-4-IHC-anti-ctbp2-antibody.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> CTBP2 was detected in frozen section of rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PA1554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1554-5.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> CTBP2 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PA1554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1554-6.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> CTBP2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PA1554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1554-7.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> CTBP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PA1554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1554-8.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PA1554). <br> CTBP2 was detected in frozen section of mouse intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PA1554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1554-ctbp2-primary-antibodies-if-testing-9.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 and Tubulin beta using anti-CTBP2 antibody (PA1554) and anti-Tubulin beta antibody (M05613-4). <br> CTBP2 and Tubulin beta was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CTBP2 Antibody (PA1554) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1554-ctbp2-primary-antibodies-fcm-testing-10.jpgAnti-C-terminal-binding protein 2 CTBP2 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-CTBP2 antibody (PA1554). <br>Overlay histogram showing HEL cells stained with PA1554 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP2 Antibody (PA1554, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-myc-antibody-pa1555-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1555-1-WB-anti-c-myc-antibody.jpgAnti-c-Myc Antibody Picoband&reg;Anti-c-Myc antibody&#44; PA1555&#44; Western blotting<br>All lanes: Anti c-Myc(PA1555) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: HEPG2 Whole Cell Lysate at 40ug<br>Predicted bind size: 49KD<br>Observed bind size: 49KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1555-fig-6-1x.jpgAnti-c-Myc Antibody Picoband&reg;CTHRC1 knockdown inhibited the Wnt/β-catenin pathway. Western blotting was used to analyze the β-catenin, Cyclin D1, and c-myc expression in CTHRC1 knockdown and control group. ***P < 0.001 . Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/15458/'>37273536</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cystathionase-antibody-pa1556-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1556-fonc-11-621806-g007.jpgAnti-Cystathionase/CTH Antibody Picoband&reg;Immunohistochemistry of FGA, F2, CFH, PIPOX, ITIH4, GNMT, MAT1A, MTHFD1, HPX, CTH, CFHR3, ENNP1, and NAT2 in clinical GBC and control specimens.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.621806/full'>33718182</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1556-cth-primary-antibodies-ihc-testing-1.jpgAnti-Cystathionase/CTH Antibody Picoband&reg;IHC analysis of Gamma Cystathionase/CTH using anti-Gamma Cystathionase/CTH antibody (PA1556). <br>Gamma Cystathionase/CTH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Gamma Cystathionase/CTH Antibody (PA1556) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1556-cth-primary-antibodies-ihc-testing-2.jpgAnti-Cystathionase/CTH Antibody Picoband&reg;IHC analysis of Gamma Cystathionase/CTH using anti-Gamma Cystathionase/CTH antibody (PA1556). <br>Gamma Cystathionase/CTH was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Gamma Cystathionase/CTH Antibody (PA1556) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1556-cth-primary-antibodies-wb-testing-1.jpgAnti-Cystathionase/CTH Antibody Picoband&reg; Western blot analysis of CTH using anti-CTH antibody (PA1556). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human THP-1 whole cell lysates, <br> Lane 4: human HCCT tissue lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTH antigen affinity purified polyclonal antibody (Catalog # PA1556) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTH at approximately 45 kDa. The expected band size for CTH is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1556-cth-primary-antibodies-fcm-testing-3.jpgAnti-Cystathionase/CTH Antibody Picoband&reg; Flow Cytometry analysis of JK cells using anti-CTH antibody (PA1556). <br>Overlay histogram showing JK cells stained with PA1556 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTH Antibody (PA1556, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1556-cth-primary-antibodies-if-testing-2.jpgAnti-Cystathionase/CTH Antibody Picoband&reg; IF analysis of CTH using anti-CTH antibody (PA1556). <br> CTH was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CTH Antibody (PA1556) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cullin-1-antibody-pa1557-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1557-1-WB-anti-cullin-1-antibody.jpgAnti-Cullin 1/Cul1 Antibody Picoband&reg;Anti-Cullin 1 antibody&#44; PA1557&#44; Western blotting<br>All lanes: Anti Cullin 1 (PA1557) at 0.5ug/ml<br>Lane 1: MM453 Whole Cell Lysate at 40ug<br>Lane 2: HT1080 Whole Cell Lysate at 40ug<br>Lane 3: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 90 KD<br>Observed bind size: 90 KD<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1557-2-IHC-anti-cullin-1-antibody.jpgAnti-Cullin 1/Cul1 Antibody Picoband&reg;Anti-Cullin 1 antibody&#44; PA1557&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dap-kinase-2-antibody-pa1558-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1558-1-WB-anti-dap-kinase-2-antibody.jpgAnti-DAP Kinase 2/DAPK2 Antibody Picoband&reg;Anti-DAP Kinase 2 antibody&#44; PA1558&#44; Western blotting<br>All lanes: Anti DAP Kinase 2 (PA1558) at 0.5ug/ml<br> Lane 1: U87 Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Lane 3: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 43KD<br> Observed bind size: 43KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-desmoglein-2-antibody-pa1559-boster.html2026-04-01T05:01:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1559-dsg2-primary-antibodies-wb-testing-1.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband&reg; Western blot analysis of DSG2 using anti-DSG2 antibody (PA1559). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: rat heart tissue lysates,<br> Lane 5: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSG2 antigen affinity purified polyclonal antibody (Catalog # PA1559) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DSG2 at approximately 160 kDa. The expected band size for DSG2 is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1559-2.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband&reg; IHC analysis of DSG2 using anti-DSG2 antibody (PA1559). <br> DSG2 was detected in paraffin-embedded section of human breast carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1559-3.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband&reg; IHC analysis of DSG2 using anti-DSG2 antibody (PA1559). <br> DSG2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1559-4.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband&reg; IHC analysis of DSG2 using anti-DSG2 antibody (PA1559). <br> DSG2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1559-5.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband&reg; IF analysis of DSG2 using anti-DSG2 antibody (PA1559). <br> DSG2 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DSG2 Antibody (PA1559) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1559-6.jpgAnti-Desmoglein 2/DSG2 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-DSG2 antibody (PA1559).<br>Overlay histogram showing 293T cells stained with PA1559 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DSG2 Antibody (PA1559,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e2f1-antibody-pa1560-boster.html2026-03-24T05:03:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1560-1-WB-anti-e2f1-antibody.jpgAnti-Transcription factor E2F1 E2F1 Antibody Picoband&reg;Anti-E2F1 antibody&#44; PA1560&#44; Western blotting<br>All lanes: Anti E2F1 (PA1560) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 47KD<br> Observed bind size: 60KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eda-antibody-pa1561-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1561-1-WB-anti-eda-eda-a2-antibody.jpgAnti-Ectodysplasin-A EDA Antibody Picoband&reg;Anti-EDA antibody&#44; PA1561&#44; Western blotting<br>WB: SW620 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lif-antibody-pa1562-boster.html2026-03-29T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1562-1-WB-anti-lif-antibody.jpgAnti-Leukemia inhibitory factor LIF Antibody Picoband&reg;Anti-LIF antibody&#44; PA1562&#44; Western blotting<br>All lanes: Anti LIF (PA1562) at 0.5ug/ml<br>Lane 1: Rat Intestine Tissue Lysate at 50ug<br>Lane 2: Rat Brain Tissue Lysate at 50ug<br>Lane 3: Rat Spleen Tissue Lysate at 50ug<br>Lane 4: Rat Thymus Tissue Lysate at 50ug<br>Lane 5: Mouse Brain Tissue Lysate at 50ug<br>Lane 6: Mouse Kidney Tissue Lysate at 50ug<br>Lane 7: Mouse Liver Tissue Lysate at 50ug<br>Predicted bind size: 22KD<br>Observed bind size: 40KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pak1-antibody-pa1563-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1563-pak1-primary-antibodies-wb-testing-1.jpgAnti-PAK1 Antibody Picoband&reg;Western blot analysis of PAK1 using anti-PAK1 antibody (PA1563). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human HEK293 whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (PA1563) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK1 at approximately 68 kDa. The expected band size for Bcl-XS/BCL2L1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1563-pak1-primary-antibodies-wb-testing-2.jpgAnti-PAK1 Antibody Picoband&reg;Western blot analysis of PAK1 using anti-PAK1 antibody (PA1563). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat lung tissue lysates,<br> Lane 3: rat stomach tissue lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse lung tissue lysates,<br> Lane 7: mouse stomach tissue lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified polyclonal antibody (PA1563) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK1 at approximately 68 kDa. The expected band size for Bcl-XS/BCL2L1 is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-scf-antibody-pa1565-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1565-1-WB-anti-scf-antibody.jpgAnti-SCF/KITLG Antibody Picoband&reg;Anti-SCF antibody&#44; PA1565&#44; Western blotting<br>All lanes: Anti SCF (PA1565) at 0.5ug/ml<br> Lane 1: Recombinant Human SCF Protein 10ng<br> Lane 2: Recombinant Human SCF Protein 5ng<br> Lane 3: Recombinant Human SCF Protein 2.5ng<br> Predicted bind size: 18.4KD<br> Observed bind size: 18.4KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1565-2-IHC-anti-scf-antibody.jpgAnti-SCF/KITLG Antibody Picoband&reg;Anti-SCF antibody&#44; PA1565&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-desmoglein-3-antibody-pa1567-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1567-dsg3-primary-antibodies-wb-testing-1.jpgAnti-Desmoglein 3/DSG3 Antibody Picoband&reg; Western blot analysis of DSG3 using anti-DSG3 antibody (PA1567). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human Hacat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DSG3 antigen affinity purified polyclonal antibody (Catalog # PA1567) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DSG3 at approximately 130 kDa. The expected band size for DSG3 is at 107 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eph-receptor-b3-antibody-pa1569-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1569-1-WB-anti-eph-receptor-b3-antibody.jpgAnti-Eph receptor B3/EPHB3 Antibody Picoband&reg;Anti-Eph receptor B3 antibody&#44; PA1569&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: A549 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1569-2-IHC-anti-eph-receptor-b3-antibody.jpgAnti-Eph receptor B3/EPHB3 Antibody Picoband&reg;Anti-Eph receptor B3 antibody&#44; PA1569&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctbp1-antibody-pa1570-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1570-1-WB-anti-ctbp1-antibody.jpgAnti-C-terminal-binding protein 1 CtBP1 Antibody Picoband&reg;Anti-CtBP1 antibody&#44; PA1570&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Testis Tissue Lysate<br>Lane 3: Rat Ovary Tissue Lysate<br>Lane 4: U87 Cell Lysate<br>Lane 5: SW620 Cell Lysate<br>Lane 6: HT1080 Cell Lysate<br>Lane 7: COLO32 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1570-2-IHC-anti-ctbp1-antibody.jpgAnti-C-terminal-binding protein 1 CtBP1 Antibody Picoband&reg;Anti-CtBP1 antibody&#44; PA1570&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1570-ctbp1-primary-antibodies-ihc-testing-3.jpgAnti-C-terminal-binding protein 1 CtBP1 Antibody Picoband&reg;IHC analysis of CTBP1 using anti-CTBP1 antibody (PA1570). <br>CTBP1 was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CTBP1 Antibody (PA1570) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-drd1-antibody-pa1571-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1571-1-WB-anti-drd1-dopamine-receptor-d1-antibody.jpgAnti-Dopamine Receptor D1/DRD1 Antibody Picoband&reg;Anti-DRD1 antibody&#44; PA1571&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1571-2-IHC-anti-drd1-dopamine-receptor-d1-antibody.jpgAnti-Dopamine Receptor D1/DRD1 Antibody Picoband&reg;Anti-DRD1 antibody&#44; PA1571&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eph-receptor-a1-antibody-pa1573-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1573-epha1-primary-antibodies-wb-testing-1.jpgAnti-Eph receptor A1/EPHA1 Antibody Picoband&reg; Western blot analysis of Eph receptor A1 using anti-Eph receptor A1 antibody (PA1573). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Colo320 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human Caco-2 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat lung tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse lung tissue lysate. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A1 antigen affinity purified polyclonal antibody (Catalog # PA1573) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A1 at approximately 113 kDa. The expected band size for Eph receptor A1 is at 108 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1573-2-IHC-anti-eph-receptor-a1-antibody.jpgAnti-Eph receptor A1/EPHA1 Antibody Picoband&reg;Anti-Eph receptor A1 antibody&#44; PA1573&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1573-3-IHC-anti-eph-receptor-a1-antibody.jpgAnti-Eph receptor A1/EPHA1 Antibody Picoband&reg;Anti-Eph receptor A1 antibody&#44; PA1573&#44; IHC(P)<br>IHC(P): Human Colon Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fascin-antibody-pa1575-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1575-1-WB-anti-fascin-antibody.jpgAnti-Fascin/FSCN1 Antibody Picoband&reg;Anti-Fascin antibody&#44; PA1575&#44; Western blotting<br>All lanes: Anti Fascin (PA1575) at 0.5ug/ml<br> Lane 1: U87 Whole Cell Lysate at 40ug<br> Lane 2: A549 Whole Cell Lysate at 40ug<br> Lane 3: MCF-7 Whole Cell Lysate at 40ug<br> Lane 4: HT1080 Whole Cell Lysate at 40ug<br> Predicted bind size: 55KD<br> Observed bind size: 55KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-foxp3-antibody-pa1577-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1577-1-WB-anti-foxp3-antibody.jpgAnti-Forkhead box protein P3 FOXP3 Antibody Picoband&reg;Anti-FOXP3 antibody&#44; PA1577&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: SGC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gaba-a-receptor-alpha-1-antibody-pa1578-boster.html2026-03-24T05:03:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1578-1-WB-anti-gaba-a-receptor-alpha-1-antibody.jpgAnti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband&reg;Anti-GABA A Receptor alpha 1 antibody&#44; PA1578&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1578-2-IHC-anti-gaba-a-receptor-alpha-1-antibody.jpgAnti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband&reg;Anti-GABA A Receptor alpha 1 antibody&#44; PA1578&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1578-gabra1-primary-antibodies-ihc-testing-3.jpgAnti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband&reg;IHC analysis of GABRA1 using anti-GABRA1 antibody (PA1578). <br>GABRA1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GABRA1 Antibody (PA1578) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1578-3.jpgAnti-GABA A Receptor alpha 1/GABRA1 Antibody Picoband&reg; Western blot analysis of GABRA1 using anti-GABRA1 antibody (PA1578). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44; <br> Lane 2: human U-87MG whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRA1 antigen affinity purified polyclonal antibody (Catalog # PA1578) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRA1 at approximately 52KD. The expected band size for GABRA1 is at 52KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-laminin-gamma-1-antibody-pa1581-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1581-1-WB-anti-laminin-gamma-1-antibody.jpgAnti-Laminin gamma 1/LAMC1 Antibody Picoband&reg;Anti-Laminin gamma 1 antibody&#44; PA1581&#44; Western blotting<br>Lane 1: Rat Kidney Tissue Lysate<br>Lane 2: Rat Lung Tissue Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: SMMC Cell Lysate<br>Lane 5: HELA Cell Lysate<br>Lane 6: SKOV1 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1581-2-IHC-anti-laminin-gamma-1-antibody.jpgAnti-Laminin gamma 1/LAMC1 Antibody Picoband&reg;Anti-Laminin gamma 1 antibody&#44; PA1581&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lamc2-antibody-pa1582-boster.html2026-03-28T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1582-1_1.jpgAnti-Laminin subunit gamma-2 LAMC2 Antibody Picoband&reg;Western blot analysis of LAMC2 using anti-LAMC2 antibody (PA1582).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human Caco-2 whole cell lysates.<br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human A549 whole cell lysates,<br> Lane 5: rat lung tissue lysates.<br> Lane 6: mouse lung tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMC2 antigen affinity purified polyclonal antibody (Catalog # PA1582) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMC2 at approximately 131KD. The expected band size for LAMC2 is at 131KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1582-2-IHC-anti-lamc2-antibody.jpgAnti-Laminin subunit gamma-2 LAMC2 Antibody Picoband&reg; IHC analysisof LAMC2 using anti-LAMC2 antibody (PA1582).<br> LAMC2 was detected in paraffin-embedded section of human lung cancertissue. Heat mediated antigen retrieval was performed inEDTA buffer (pH8.0, epitope retrieval solution). Thetissue section was blocked with 10% goat serum. The tissue sectionwasthen incubated with 1μg/ml rabbit anti-LAMC2 Antibody (PA1582) overnight at 4°C. Biotinylated goat anti-rabbitIgG was used assecondary antibody and incubated for 30 minutes at 37°C. The tissuesection was developed using Strepavidin-Biotin-Complex (SABC)(Catalog# SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prostaglandin-e-receptor-ep1-antibody-pa1583-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1583-ptger1-primary-antibodies-wb-testing-1.jpgAnti-Prostaglandin E Receptor EP1/PTGER1 Antibody Picoband&reg; Western blot analysis of PTGER1 using anti-PTGER1 antibody (PA1583). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human HEL whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGER1 antigen affinity purified polyclonal antibody (Catalog # PA1583) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGER1 at approximately 42 kDa. The expected band size for PTGER1 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dopamine-receptor-d3-antibody-pa1584-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1584-drd3-primary-antibodies-wb-testing-1.jpgAnti-Dopamine Receptor D3/DRD3 Antibody Picoband&reg;Western blot analysis of DRD3 using anti-DRD3 antibody (PA1584). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD3 antigen affinity purified polyclonal antibody (PA1584) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRD3 at approximately 59 kDa. The expected band size for DRD3 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1584-drd3-primary-antibodies-ihc-testing-1.jpgAnti-Dopamine Receptor D3/DRD3 Antibody Picoband&reg;IHC analysis of DRD3 using anti-DRD3 antibody (PA1584). <br>DRD3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRD3 Antibody (PA1584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1584-drd3-primary-antibodies-ihc-testing-2.jpgAnti-Dopamine Receptor D3/DRD3 Antibody Picoband&reg;IHC analysis of DRD3 using anti-DRD3 antibody (PA1584). <br>DRD3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRD3 Antibody (PA1584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sparc-antibody-pa1585-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1585-sparc-primary-antibodies-wb-testing-1.jpgAnti-SPARC Antibody Picoband&reg;Western blot analysis of SPARC using anti-SPARC antibody (PA1585). <br> Electrophoresis was performed on a 108% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: human U2OS whole cell lysates,<br> Lane 3: rat testis tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse testis tissue lysates,<br> Lane 6: mouse C2C12 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (PA1585) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SPARC at approximately 38 kDa. The expected band size for SPARC is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trem1-antibody-pa1586-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1586-2-IHC-anti-trem-1-antibody.jpgAnti-TREM1 Antibody Picoband&reg;Anti-TREM1 antibody&#44; PA1586&#44; IHC(P)<br>IHC(P): Mouse Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1586-1_1-WB-anti-trem-1-antibody.jpgAnti-TREM1 Antibody Picoband&reg;Anti-TREM1 antibody&#44; PA1586&#44; Western blotting<br>Lane 1: Recombinant Mouse TREM1 Protein 1nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1586-fig-4-1x.jpgAnti-TREM1 Antibody Picoband&reg;HMGN1, TLR4, and TREM-1 protein levels in the kidney of different experimental groups. (A) Immunohistochemical stain of HMGN1, TLR4 and TREM-1 in the kidney tissue of UUO rats ( n = 5). Original magnification = 400 ×. Scale bars. 50 um. (B) Quantification of the levels of HMGN1, TLR4 and TREM-1 proteins. all values are presented as mean ± SEM. ∗∗ p < 0.001, ∗∗∗ p < 0.0001. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/14765/'>36691481</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1586-fig-5-1x.jpgAnti-TREM1 Antibody Picoband&reg;Correlation analysis of HMGN1 protein with the key proteins involved in UUO-induced renal inflammation and fibrosis. (A) HMGN1 protein had a positive relation with F4/80+ cells. (B) HMGN1 protein was positively associated with Mcp-1 protein. (C) HMGN1 protein was positively related to KIM-1 protein. (D) HMGN1 protein was positively associated with α -SMA protein. (E) HMGN1 protein was positively associated with β -catenin protein. (F) TLR4 protein and HMGN1 protein had a positive relation. (G) TLR4 protein was positively associated with TREM-1 protein. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/14765/'>36691481</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1586-3.jpgAnti-TREM1 Antibody Picoband&reg; Western blot analysis of Trem1 using anti-Trem1 antibody (PA1586). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates&#44; <br> Lane 2: mouse liver tissue lysates&#44; <br> Lane 3: mouse spleen tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Trem1 antigen affinity purified polyclonal antibody (Catalog # PA1586) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Trem1 at approximately 22-26KD. The expected band size for Trem1 is at 26KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-granzyme-a-antibody-pa1588-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1588-1-WB-anti-granzyme-a-antibody.jpgAnti-Granzyme A/GZMA Antibody Picoband&reg;Anti-Granzyme A antibody&#44; PA1588&#44; Western blotting<br>Lane 1: JURKAT Cell Lysate<br>Lane 2: CEM Cell Lysate<br>Lane 3: RAJI Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1588-2-IHC-anti-granzyme-a-antibody.jpgAnti-Granzyme A/GZMA Antibody Picoband&reg;Anti-Granzyme A antibody&#44; PA1588&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gst3-gst-pi-antibody-pa1590-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1590-1-IHC-anti-gst3-gst-pi-antibody.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg;Anti-GST3/GST pi antibody&#44; PA1590&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1590-2-WB-anti-gst3-gst-pi-antibody.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg;Anti-GST3/GST pi antibody&#44; PA1590&#44;Western blotting<br>All lanes: Anti GST3/GST pi (PA1590) at 0.5ug/ml<br>Lane 1: Rat Kidney Tissue Lysate at 50ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 23KD<br>Observed bind size: 23KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1590-10-1055-s-0044-1801320-i231630-2.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg;( A ) Control ( B ) Antrochoanal polyp (ACP) patient. The expression of GSTP1 in ACP patients and controls is not statistically different. (GSTP1; original magnification: x200). (Control; original magnification: x150).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.thieme-connect.com/products/ejournals/html/10.1055/s-0044-1801320'>40443552</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdac8-antibody-pa1591-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1591-1-WB-anti-hdac8-histone-deacetylase-8-antibody.jpgAnti-Histone deacetylase 8 HDAC8 Antibody Picoband&reg;Anti-HDAC8 antibody&#44; PA1591&#44; Western blotting<br>All lanes: Anti HDAC8 (PA1591) at 0.5ug/ml<br> Lane 1: Rat Cardiac Muscle: Tissue Lysate at 50ug<br> Lane 2: A549 Whole Cell Lysate at 40ug<br> Predicted bind size: 42KD<br> Observed bind size: 42KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1591-2-IHC-anti-hdac8-histone-deacetylase-8-antibody.jpgAnti-Histone deacetylase 8 HDAC8 Antibody Picoband&reg;Anti-HDAC8 antibody&#44; PA1591&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd168-antibody-pa1592-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1592-1-WB-anti-cd168-antibody.jpgAnti-CD168/HMMR Antibody Picoband&reg;Anti-CD168 antibody&#44; PA1592&#44; Western blotting<br>Lane 1: MM231 Cell Lysate<br>Lane 2: MM453 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: A549 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1592-2-IHC-anti-cd168-antibody.jpgAnti-CD168/HMMR Antibody Picoband&reg;Anti-CD168 antibody&#44; PA1592&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-foxp1-antibody-pa1593-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1593-1-WB-anti-foxp1-antibody.jpgAnti-Forkhead box protein P1 FOXP1 Antibody Picoband&reg;Anti-FOXP1 antibody&#44; PA1593&#44; Western blotting<br>Lane 1: Rat Spleen Tissue Lysate<br>Lane 2: COLO320 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1593-2-IHC-anti-foxp1-antibody.jpgAnti-Forkhead box protein P1 FOXP1 Antibody Picoband&reg;Anti-FOXP1 antibody&#44; PA1593&#44; IHC(P)<br>IHC(P): Human Rectal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nr3c2-antibody-pa1594-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1594-1_1.jpgAnti-Mineralocorticoid Receptor/NR3C2 Antibody Picoband&reg;Lane 1: 293T Cell Lysate Lane 2: SMMC Cell Lysate Lane 3: SW620 Cell Lysate Lane 4: HELA Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-9-antibody-pa1595-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1595-1-WB-anti-caspase-9-antibody.jpgAnti-Caspase 9/CASP9 Antibody Picoband&reg;Anti-Caspase-9 antibody&#44; PA1595&#44; Western blotting<br>All lanes: Anti Caspase-9 (PA1595) at 0.5ug/ml<br>Lane 1: SMMC Whole Cell Lysate at 40ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Lane 3: CEM Whole Cell Lysate at 40ug<br>Lane 4: JURKAT Whole Cell Lysate at 40ug<br>Lane 5: RAJI Whole Cell Lysate at 40ug<br>Lane 6: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 35KD<br>Observed bind size: 35KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1595-2-IHC-anti-caspase-9-antibody.jpgAnti-Caspase 9/CASP9 Antibody Picoband&reg;Anti-Caspase-9 antibody&#44; PA1595&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1595-fphar-12-684915-g002.jpgAnti-Caspase 9/CASP9 Antibody Picoband&reg;PPM-18 triggers apoptotic cell death in bladder cancer cells. (A) T24 and EJ cells were treated with the indicated concentration of PPM-18, and stained with annexin V-FITC and PI. The apoptotic effect of PPM-18 on T24 and EJ cells was measured by flow cytometry. (B) Western blotting showed the effect of PPM-18 on the expression of cleaved caspase-9, 3, and PARP in T24 and EJ cells. (C) T24 and EJ cells were exposed to 10 μM PPM-18 for 24 h, and stained with JC-1 fluorescent probe. The effect of PPM-18 on the alteration of mitochondria membrane potential was analyzed by flow cytometry. (D) Western blotting displayed the effect of PPM-18 on expression of BAX and BCL-2 in T24 and EJ cells. (E) and (F) The effect of Z-VAD-FMK on PPM-18 reduced the viability of T24 and EJ cells, or PPM-18 triggered apoptosis in T24 cells. Cells treated with 15 μM PPM-18 combined with or without 20 μM Z-VAD-FMK for 24 h, and cell viability and apoptosis were respectively measured by MTS assay and flow cytometry. Data are presented as the mean ± SD of at least three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the control group, or vs. PPM-18+Z-VAD-FMK.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.684915/full'>34305598</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1595-fphar-12-684915-g004.jpgAnti-Caspase 9/CASP9 Antibody Picoband&reg;PPM-18 induces AMPK-dependent autophagy and apoptotic cell death in bladder cancer cells. (A) and (B) T24 and EJ cells were exposed to the indicated concentration of PPM-18 for 24 h, and the expression of phospho-AMPK, AMPK, phospho-mTORC1, phospho-P70S6K, phospho-PI3K, PI3K, phospho-AKT, and AKT were analyzed by western blot. (C) and (D) Western blot showed the effect of AMPK siRNA or 5 μM Compound C on the phosphorylation of AMPK, mTORC1, and P70S6K, and the expression of LC3B and cleaved caspase-9, 3 in PPM-18–treated T24 cells. (E) and (F) MTS assay and flow cytometry demonstrated that treatment with Compound C affected PPM-18–reduced viability of T24 cells and PPM-18–induced apoptosis in T24 cells. Data are presented as the mean ± SD of at least three independent experiments. *** p < 0.001 vs. the control group, or vs. PPM-18+Compund C.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.684915/full'>34305598</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1595-fphar-12-684915-g003.jpgAnti-Caspase 9/CASP9 Antibody Picoband&reg;PPM-18 stimulates autophagy that promotes apoptosis in bladder cancer cells. (A) T24 and EJ cells were treated with various concentrations of PPM-18 for 24 h. Then cells were harvested for western blotting to detect the expression of p62 and LC3B, two autophagy indicators. (B) The formation of autophagosomes in T24 and EJ cells treated with or without 10 μM PPM-18 for 18 h under the transmission electron microscope. Arrows indicated the autophagosomes containing intact and degraded cellular debris. Scale bar: left: 5.0 μm, middle: 2.0 μm, and right: 1.0 μm. (C) Western blot showed the effect of 5 mM 3-MA, a typical autophagy inhibitor, on the expression of p62, LC3B, and cleaved caspase-9, 3 in PPM-18–treated T24 and EJ cells. (D) and (E) T24 and EJ cells were treated with 15 μM PPM-18 combined with or without 5 mM 3-MA, and the effect of 3-MA on PPM-18–reduced viability or PPM-18–triggered apoptosis in T24 cells and EJ cells were measured by MTS assay and flow cytometry, respectively. (F) T24 and EJ cells were treated with 15 μM PPM-18 combined with or without 10 μM rapamycin, a typical autophagy agonist, and the effect of rapamycin on PPM-18–induced autophagy and apoptosis were evaluated by western blot. (G) The MTS assay demonstrated that rapamycin affected PPM-18–reduced viability in T24 and EJ cells. (H) Flow cytometry displayed the effect of rapamycin on PPM-18–triggered apoptosis in T24 and EJ cells. Data are presented as the mean ± SD of at least three independent experiments. ** p < 0.01 and *** p < 0.001 vs. the control group, or vs. PPM-18+3-MA. * p < 0.05 and *** p < 0.001 vs. PPM-18+Rapa.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.684915/full'>34305598</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd45-antibody-pa1596-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1596-1-WB-anti-cd45-antibody.jpgAnti-CD45/PTPRC Antibody Picoband&reg;Anti-CD45 antibody&#44; PA1596&#44; Western blotting<br>All lanes: Anti CD45 (PA1596) at 0.5ug/ml<br>Lane 1: JURKAT Whole Cell Lysate at 40ug<br>Lane 2: CEM Whole Cell Lysate at 40ug<br>Predicted bind size: 147KD<br>Observed bind size: 147KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1596-2-IHC-anti-cd45-antibody.jpgAnti-CD45/PTPRC Antibody Picoband&reg;Anti-CD45 antibody&#44; PA1596&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1596-3-IF-anti-cd45-antibody.jpgAnti-CD45/PTPRC Antibody Picoband&reg;Anti-CD45 antibody&#44; PA1596&#44; ICC<br>ICC: JURKAT Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1596-4.jpgAnti-CD45/PTPRC Antibody Picoband&reg; IF analysis of CD45 using anti-CD45 antibody (PA1596) <br> CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD45 Antibody (PA1596) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdj2-antibody-pa1597-boster.html2026-03-24T05:03:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1597-1_1.jpgAnti-HDJ2/DNAJA1 Antibody Picoband&reg;Anti-HDJ2 antibody&#44; PA1597&#44; Western blotting<br>All lanes: Anti HDJ2 (PA1597) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Rat Lung Tissue Lysate at 50ug<br>Lane 3: Mouse Brain Tissue Lysate at 50ug<br>Lane 4: Mouse Lung Tissue Lysate at 50ug<br>Lane 5: U87 Whole Cell Lysate at 40ug<br>Lane 6: A549 Whole Cell Lysate at 40ug<br>Lane 7: COLO320 Whole Cell Lysate at 40ug<br>Lane 8: A431 Whole Cell Lysate at 40ug<br>Lane 9: HT1080 Whole Cell Lysate at 40ug<br>Predicted bind size: 45KD<br>Observed bind size: 45KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1597-dnaja1-primary-antibodies-if-testing-2.jpgAnti-HDJ2/DNAJA1 Antibody Picoband&reg; IF analysis of DNAJA1 using anti-DNAJA1 antibody (PA1597). <br> DNAJA1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-DNAJA1 Antibody (PA1597) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-haptoglobin-antibody-pa1599-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1599-hp-primary-antibodies-wb-testing-1.jpgAnti-Haptoglobin/HP Antibody Picoband&reg; Western blot analysis of Haptoglobin/HP using anti-Haptoglobin/HP antibody (PA1599). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Haptoglobin/HP antigen affinity purified polyclonal antibody (Catalog # PA1599) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Haptoglobin/HP at approximately 45 kDa. The expected band size for Haptoglobin/HP is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1599-2-IHC-anti-haptoglobin-antibody.jpgAnti-Haptoglobin/HP Antibody Picoband&reg;Anti-Haptoglobin antibody&#44; PA1599&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1599-41598_2019_43039_fig2_html.pngAnti-Haptoglobin/HP Antibody Picoband&reg;Effects of sample fixation on the retention of proteins of the method using PVDF membranes. ( a ) Indicated numerals are amounts (5.0, 2.5, 1.3, and 0.6 μg) of the pooled serum proteins were subjected to 10% SDS-PAGE. The blotted membranes were treated using the traditional (left panel) or optimised fixation protocol (right). ( b ) Staining intensities were statistically analyzed (n = 3 individual experiments). Solid bar, no fixation; White bar, optimised fixation protocol. The exposure times were the same in all procedures. Band intensities were analysed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. * Significantly different p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. All values are means ± S.E. (error bars). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-019-43039-3'>31040299</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1599-41598_2019_43039_fig3_html.pngAnti-Haptoglobin/HP Antibody Picoband&reg;Fixation-dependent differences in lectin staining intensities when using PVDF and nitrocellulose membranes. Pooled human serum proteins (3 μg) were separated on 10% SDS-PAGE, and the proteins were transferred onto PVDF and nitrocellulose membranes, the whole membrane was cutted into five pieces for subsequently fixation treatments and followed by staining with lectins (LCA and SNA). Lane i, CBB staining; lane ii, no fixation; lane iii, drying at room temperature; lane iv, sample heating at 100 °C; lane v, organic solvent (acetone and 50% methanol for PVDF and nitrocellulose membranes, respectively) treatments at room temperature; lane vi, organic solvent treatments followed by sample heating at 100 °C. All treatments were applied for 30 min. Left, WB pattern; right, quantitative analysis (n = 3 individual experiments). The exposure times were the same in all procedures. Band intensities were analysed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. ** Significantly different p < 0.01, *** p < 0.001, **** p < 0.0001. All values are means ± S.E. (error bars). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-019-43039-3'>31040299</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1599-41598_2019_43039_fig1_html.pngAnti-Haptoglobin/HP Antibody Picoband&reg;Fixation method-dependent differences in the immunostaining intensity. ( a ) Pooled human serum samples (10 μg) were analysed by 10% SDS-PAGE, and the separated proteins were transferred onto PVDF (top) and nitrocellulose (bottom) membranes, the whole membrane was cutted into eight pieces for subsequently fixation treatments. Representative images, showing anti-human IgG antibody staining after the following treatments: lane i, Coomassie Brilliant Blue (CBB) staining; lane ii, no fixation; lane iii, drying at room temperature; lane iv, heating at 50 °C; lane v, heating at 100 °C; lane vi, immersion into the organic solvents (acetone and 50% methanol for PVDF and nitrocellulose membranes, respectively) at room temperature; lane vii, immersion into the organic solvents at 0 °C; lane viii, immersion into the organic solvents at 0 °C followed by sample heating at 100 °C; lane ix, immersion into the organic solvents at 0 °C followed by sample heating at 50 °C. All treatments were performed for 30 min. The relative intensity was shown on the right (n = 3 individual experiments). ( b ) Proteins were separated by 10% SDS-PAGE, and transferred onto PVDF membranes, the whole membrane was cutted into five pieces for subsequently fixation treatments. Fixed in acetone at 0 °C, and heated at different temperatures for 30 min prior to the immunostaining. Lane i, CBB staining; lane ii, no fixation; lane iii, heating at 25 °C; lane iv, heating at 50 °C; lane v, heating at 75 °C; lane vi, heating at 100 °C. Right panel shows the relative intensity at different temperatures. The exposure times were the same in all procedures. Band intensities were analysed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. ** Significantly different p < 0.01, *** p < 0.001, **** p < 0.0001. All values are means ± S.E. (error bars). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-019-43039-3'>31040299</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1599-41598_2019_43039_fig4_html.pngAnti-Haptoglobin/HP Antibody Picoband&reg;Sensitivity of the LB method coupled with the fixation step, when using PVDF membranes. Indicated numerals are amounts (3.0, 1.5, 0.7, 0.3, and 0.1 μg) of the pooled serum proteins were subjected to 10% SDS-PAGE. ( a ) The blotted membranes were treated using the traditional (top panel) or optimised fixation protocol (bottom panel). ( b ) Quantification of band intensities were statistically analyzed (n = 3 individual experiments). Solid bar, no fixation; White bar, sample fixation. The exposure times were the same for the same lectin blotting using fixation or no fixation. Band intensities were analysed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. * Significantly different p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. All values are means ± S.E. (error bars). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-019-43039-3'>31040299</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1599-41598_2019_43039_fig5_html.pngAnti-Haptoglobin/HP Antibody Picoband&reg;Application of the optimised immunostaining and lectin staining methods. ( a ) CFTR levels in HT-29 cells. ( b ) HIF-1α levels in HEK-293T cells. ( c ) GAPDH levels in liver tissue of mouse. Various amounts (quantity represented in μg) of total cellular proteins analysed using 8% SDS-PAGE and immunostained using PVDF membrane and treated with or without fixation treatments. ( d ) AFP levels in the sera of healthy volunteers (n = 6) and HCC patients (n = 7), with different sample volumes using the PVDF membranes, with or without the fixation. ** Significantly different p < 0.01, *** p < 0.001, **** p < 0.0001. All values are means ± S.E. (error bars). ( e ) AAL and PHA-E staining, using 6 μg of proteins from the sera of healthy volunteers (n = 6) and prostate cancer patients (PC, n = 10), blotted on PVDF membranes, with or without fixation. Three representative healthy samples (lane i, ii, iii) and seven representative prostate cancer samples (lane iv-x) are presented (left). Boxplot provides the quantification of the total band intensities (right). Circle, healthy subjects; square, prostate cancer patients. Student’s t-test. ** P < 0.01 and **** P < 0.0001, healthy subjects vs. PC patients; # P < 0.0001, No fixation vs fixation groups. Band intensities were compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-019-43039-3'>31040299</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdac3-antibody-pa1600-1-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1600-1-1-IHC-anti-hdac3-antibody.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; IHC analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> HDAC3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HDAC3 Antibody (PA1600-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1600-1-2-IHC-anti-hdac3-antibody.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; IHC analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> HDAC3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HDAC3 Antibody (PA1600-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1600-1-3-IHC-anti-hdac3-antibody.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; IHC analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> HDAC3 was detected in paraffin-embedded section of Rat Intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HDAC3 Antibody (PA1600-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1600-1-4-IHC-anti-hdac3-antibody.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; IHC analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> HDAC3 was detected in frozen section of Mouse Brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HDAC3 Antibody (PA1600-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1600-1-5-IF-anti-hdac3-antibody.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; IHC analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> HDAC3 was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-HDAC3 Antibody (PA1600-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1600-1-6-WB-anti-hdac3-antibody.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; Western blot analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Stomach Tissue Lysate<br> Lane 2: Rat Testis Tissue Lysate<br> Lane 3: MCF-7 Cell Lysate<br> Lane 4: HELA Cell Lysate<br> Lane 5: JURKAT Cell Lysate<br> Lane 6: SKOV Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (Catalog # PA1600-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1600-1-7.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; IF analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> HDAC3 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HDAC3 Antibody (PA1600-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1600-1-8.jpgAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; IF analysis of HDAC3 using anti-HDAC3 antibody (PA1600-1). <br> HDAC3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HDAC3 Antibody (PA1600-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1600-1-9.pngAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-HDAC3 antibody (PA1600-1). <br> Overlay histogram showing A431 cells stained with PA1600-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (PA1600-1&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1600-1-10.pngAnti-Histone deacetylase 3 HDAC3 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-HDAC3 antibody (PA1600-1). <br> Overlay histogram showing U937 cells stained with PA1600-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (PA1600-1&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hoxa3-antibody-pa1602-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1602-1-WB-anti-hoxa3-antibody.jpgAnti-Homeobox protein Hox-A3 HOXA3 Antibody Picoband&reg;Anti-HOXA3 antibody&#44; PA1602&#44; Western blotting<br>All lanes: Anti HOXA3 (PA1602) at 0.5ug/ml<br>WB: SW620 Whole Cell Lysate at 40ug<br>Predicted bind size: 46KD<br>Observed bind size: 46KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1602-2-IHC-anti-hoxa3-antibody.jpgAnti-Homeobox protein Hox-A3 HOXA3 Antibody Picoband&reg;Anti-HOXA3 antibody&#44; PA1602&#44; IHC(P)<br>IHC(P): Human Pancreatic Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hoxa4-antibody-pa1603-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1603-1-WB-anti-hoxa4-antibody.jpgAnti-Homeobox protein Hox-A4 HOXA4 Antibody Picoband&reg;Anti-HOXA4 antibody&#44; PA1603&#44; Western blotting<br>Lane 1: SW620 Cell Lysate <br>Lane 2: SW620 Cell Lysate<br>Lane 3: PC-12 Cell Nuclear Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1603-2-IHC-anti-hoxa4-antibody.jpgAnti-Homeobox protein Hox-A4 HOXA4 Antibody Picoband&reg;Anti-HOXA4 antibody&#44; PA1603&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1603-3-IF-anti-hoxa4-antibody.jpgAnti-Homeobox protein Hox-A4 HOXA4 Antibody Picoband&reg;Anti-HOXA4 antibody&#44; PA1603&#44; ICC<br>ICC: HELA Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsd17b2-antibody-pa1604-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1604-1-WB-anti-hsd17b2-antibody.jpgAnti-HSD17B2 Antibody Picoband&reg;Anti-HSD17B2 antibody&#44; PA1604&#44; Western blotting<br>All lanes: Anti HSD17B2 (PA1604) at 0.5ug/ml<br>WB: Human Placenta Tissue Lysate at 50ug<br>Predicted bind size: 43KD<br>Observed bind size: 43KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1604-2-IHC-anti-hsd17b2-antibody.jpgAnti-HSD17B2 Antibody Picoband&reg;Anti-HSD17B2 antibody&#44; PA1604&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1604-3-IHC-anti-hsd17b2-antibody.jpgAnti-HSD17B2 Antibody Picoband&reg;Anti-HSD17B2 antibody&#44; PA1604&#44; IHC(F)<br>IHC(F): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hoxa6-antibody-pa1605-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1605-1-WB-anti-hoxa6-antibody.jpgAnti-Homeobox protein Hox-A6 HOXA6 Antibody Picoband&reg;Anti-HOXA6 antibody&#44; PA1605&#44; Western blotting<br>Lane 1: SW620 cell Lysate<br>Lane 2: SW620 cell Lysate<br>Lane 3: HELA cell Lysate<br>Lane 4: HT1080 cell Lysate<br>Lane 5: HT1080 cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsf2-antibody-pa1607-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1607-hsf2-primary-antibodies-wb-testing-1.jpgAnti-Heat shock factor protein 2 HSF2 Antibody Picoband&reg; Western blot analysis of HSF2 using anti-HSF2 antibody (PA1607). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: rat thymus tissue lysates,<br> Lane 4: mouse thymus tisue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF2 antigen affinity purified polyclonal antibody (Catalog # PA1607) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF2 at approximately 60 kDa. The expected band size for HSF2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1607-3-IHC-anti-hsf2-antibody.jpgAnti-Heat shock factor protein 2 HSF2 Antibody Picoband&reg;Anti-HSF2 antibody&#44; PA1607&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1607-2-IHC-anti-hsf2-antibody.jpgAnti-Heat shock factor protein 2 HSF2 Antibody Picoband&reg;Anti-HSF2 antibody&#44; PA1607&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1607-4.jpgAnti-Heat shock factor protein 2 HSF2 Antibody Picoband&reg; IF analysis of HSF2 using anti-HSF2 antibody (PA1607). <br> HSF2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSF2 Antibody (PA1607) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1607-5.jpgAnti-Heat shock factor protein 2 HSF2 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-HSF2 antibody (PA1607).<br>Overlay histogram showing 293T cells stained with PA1607 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSF2 Antibody (PA1607,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp105-antibody-pa1608-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1608-1-WB-anti-hsp105-antibody.jpgAnti-Hsp105/HSPH1 Antibody Picoband&reg;Anti-Hsp105 antibody&#44; PA1608&#44; Western blotting<br>Lane 1: Rat Ovary Tissue Lysate <br>Lane 2: A549 Cell Lysate <br>Lane 3: U87 Cell Lysate <br>Lane 4: HELA Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1608-2-IHC-anti-hsp105-antibody.jpgAnti-Hsp105/HSPH1 Antibody Picoband&reg;Anti-Hsp105 antibody&#44; PA1608&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1608-3-IHC-anti-hsp105-antibody.jpgAnti-Hsp105/HSPH1 Antibody Picoband&reg;Anti-Hsp105 antibody&#44; PA1608&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1608-4-IHC-anti-hsp105-antibody.jpgAnti-Hsp105/HSPH1 Antibody Picoband&reg;Anti-Hsp105 antibody&#44; PA1608&#44; IHC(F)<br>IHC(F): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1608-hsp105-primary-antibody-if-testing-5.jpgAnti-Hsp105/HSPH1 Antibody Picoband&reg; IF analysis of HSPH1 using anti-HSPH1 antibody (PA1608). <br> HSPH1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HSPH1 Antibody (PA1608) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1608-hsp105-primary-antibody-fcm-testing-6.pngAnti-Hsp105/HSPH1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-HSPH1 antibody (PA1608). <br>Overlay histogram showing A431 cells stained with PA1608 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPH1 Antibody (PA1608, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ido1-antibody-pa1611-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1611-1-WB-anti-ido1-ido-antibody.jpgAnti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband&reg;Anti-IDO1 antibody&#44; PA1611&#44; Western blotting<br>All lanes: Anti IDO1 (PA1611) at 0.5ug/ml<br> Lane 1: SMMC Whole Cell Lysate at 40ug<br> Lane 2: A549 Whole Cell Lysate at 40ug<br> Lane 3: Human Placenta Tissue Lysate at 50ug<br> Lane 4: SW620 Whole Cell Lysate at 40ug<br> Lane 5: U87 Whole Cell Lysate at 40ug<br> Lane 6: 293T Whole Cell Lysate at 40ug<br> Lane 7: A431 Whole Cell Lysate at 40ug<br> Lane 8: HELA Whole Cell Lysate at 40ug<br> Lane 9: COLO320 Whole Cell Lysate at 40ug<br> Predicted bind size: 45KD<br> Observed bind size: 45KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-indol1-antibody-pa1612-boster.html2026-03-24T05:03:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1612-1_2.jpgAnti-INDOL1/IDO2 Antibody Picoband&reg; Western blot analysis of IDO2 using anti-IDO2 antibody (PA1612). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates (positive control)&#44;<br> Lane 2: human placenta tissue lysates (positive control)&#44;<br> Lane 3: human Caco-2 whole cell lysates (positive control)&#44;<br> Lane 4: human PC-3 whole cell lysates (negative control). <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO2 antigen affinity purified polyclonal antibody (Catalog # PA1612) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDO2 at approximately 47KD. The expected band size for IDO2 is at 47KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsd17b1-antibody-pa1613-1-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1613-1-hsd17b1-primary-antibodies-wb-testing-1.jpgAnti-HSD17B1 Antibody Picoband&reg; Western blot analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human T47D whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: rat C6 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B1 antigen affinity purified polyclonal antibody (Catalog # PA1613-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B1 at approximately 37-38 kDa. The expected band size for HSD17B1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1613-1-2-ihc-anti-hsd17b1-17-beta-hsd1-antibody.jpgAnti-HSD17B1 Antibody Picoband&reg; IHC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1). <br> HSD17B1 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1613-1-3-IHC-anti-hsd17b1-17-beta-hsd1-antibody.jpgAnti-HSD17B1 Antibody Picoband&reg; IHC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1). <br> HSD17B1 was detected in a paraffin-embedded section of Rat Liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1613-1-4-IHC-anti-hsd17b1-17-beta-hsd1-antibody.jpgAnti-HSD17B1 Antibody Picoband&reg; IHC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1). <br> HSD17B1 was detected in a paraffin-embedded section of Rat Ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1613-1-5-if-anti-hsd17b1-17-beta-hsd1-antibody.jpgAnti-HSD17B1 Antibody Picoband&reg; ICC analysis of HSD17B1 using anti-HSD17B1 antibody (PA1613-1). <br> HSD17B1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-HSD17B1 Antibody (PA1613-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikk-alpha-antibody-pa1614-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1614-ikka-primary-antibodies-wb-testing-1.jpgAnti-IKK alpha/CHUK Antibody Picoband&reg; Western blot analysis of IKK Alpha/CHUK using anti-IKK Alpha/CHUK antibody (PA1614). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK Alpha/CHUK antigen affinity purified polyclonal antibody (Catalog # PA1614) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK Alpha/CHUK at approximately 85 kDa. The expected band size for IKK Alpha/CHUK is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1614-ikka-primary-antibodies-fcm-testing-2.jpgAnti-IKK alpha/CHUK Antibody Picoband&reg; Flow Cytometry analysis of Raji cells using anti-IKK Alpha/CHUK antibody (PA1614). <br> Overlay histogram showing Raji cells stained with PA1614 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK Alpha/CHUK Antibody (PA1614, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il2rb-antibody-pa1615-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1615-1-WB-anti-il2rb-il-2rbeta-antibody.jpgAnti-IL2 Receptor beta/IL2RB Antibody Picoband&reg;Anti-IL2RB antibody&#44; PA1615&#44; Western blotting<br>Lane 1: PANC Cell Lysate <br>Lane 2: HELA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il2-receptor-gamma-antibody-pa1616-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1616-1-WB-anti-il2-receptor-gamma-antibody.jpgAnti-IL2 Receptor gamma/IL2RG Antibody Picoband&reg;Anti-IL2 Receptor gamma antibody&#44; PA1616&#44; Western blotting<br>Lane 1: PANC Cell Lysate <br>Lane 2: HELA Cell Lysate <br>Lane 3: JURKAT Cell Lysate <br>Lane 4: RAJI Cell Lysate <br>Lane 5: CEM Cell Lysate <br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il3ra-antibody-pa1617-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1617-1-WB-anti-il3ra-cd123-antibody.jpgAnti-IL3RA Antibody Picoband&reg;Anti-IL3RA antibody&#44; PA1617&#44; Western blotting<br>Lane 1: A431 Cell Lysate <br>Lane 2: SMMC Cell Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: 293T Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il5ra-antibody-pa1619-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1619-1-WB-anti-il5ra-cd125-antibody.jpgAnti-IL5RA Antibody Picoband&reg;Anti-IL5RA antibody&#44; PA1619&#44; Western blotting<br>All lanes: Anti IL5RA (PA1619) at 0.5ug/ml<br> Lane 1: A549 Whole Cell Lysate at 40ug<br> Lane 2: SMMC Whole Cell Lysate at 40ug<br> Lane 3: U87 Whole Cell Lysate at 40ug<br> Lane 4: 293T Whole Cell Lysate at 40ug<br> Lane 5: PANC Whole Cell Lysate at 40ug<br> Lane 6: HELA Whole Cell Lysate at 40ug<br> Lane 7: JURKAT Whole Cell Lysate at 40ug<br> Lane 8: RAJI Whole Cell Lysate at 40ug<br> Lane 9: CEM Whole Cell Lysate at 40ug<br> Predicted bind size: 48KD<br> Observed bind size: 60KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-insulin-receptor-antibody-pa1620-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1620-1-WB-anti-insulin-receptor-antibody.jpgAnti-Insulin Receptor/INSR Antibody Picoband&reg;Anti-Insulin Receptor antibody&#44; PA1620&#44; Western blotting<br>All lanes: Anti Insulin Receptor(PA1620) at 0.5ug/ml<br>Lane 1: Rat Kidney Tissue Lysate at 50ug<br>Lane 2: PANC Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 155KD<br>Observed bind size: 155KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-alpha-3-antibody-pa1621-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1621-itga3-primary-antibodies-wb-testing-1.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband&reg; Western blot analysis of ITGA3 using anti-ITGA3 antibody (PA1621). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human U2OS whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: rat PC-12 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA3 antigen affinity purified polyclonal antibody (Catalog # PA1621) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA3 at approximately 130 kDa. The expected band size for ITGA3 is at 117 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1621-itga3-primary-antibodies-ihc-testing-2.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband&reg; IHC analysis of ITGA3 using anti-ITGA3 antibody (PA1621). <br> ITGA3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGA3 Antibody (PA1621) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1621-itga3-primary-antibodies-ihc-testing-3.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband&reg; IHC analysis of ITGA3 using anti-ITGA3 antibody (PA1621). <br> ITGA3 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGA3 Antibody (PA1621) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1621-itga3-primary-antibodies-if-testing-4_1.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband&reg; IF analysis of ITGA3 using anti-ITGA3 antibody (PA1621). <br> ITGA3 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ITGA3 Antibody (PA1621) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1621-itga3-primary-antibodies-fcm-testing-5_1.jpgAnti-Integrin alpha 3/ITGA3 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-ITGA3 antibody (PA1621). <br> Overlay histogram showing U87 cells stained with PA1621 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ITGA3 Antibody (PA1621, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd18-antibody-pa1623-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1623-itgb2-primary-antibodies-wb-testing-1.jpgAnti-CD18/ITGB2 Antibody Picoband&reg; Western blot analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PA1623). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18/ITGB2 antigen affinity purified polyclonal antibody (Catalog # PA1623) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18/ITGB2 at approximately 90-100 kDa. The expected band size for CD18/ITGB2 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1623-itgb2-primary-antibodies-ihc-testing-2.jpgAnti-CD18/ITGB2 Antibody Picoband&reg; IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PA1623). <br> CD18/ITGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD18/ITGB2 Antibody (PA1623) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1623-itgb2-primary-antibodies-ihc-testing-3.jpgAnti-CD18/ITGB2 Antibody Picoband&reg; IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PA1623). <br> CD18/ITGB2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD18/ITGB2 Antibody (PA1623) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tim-1-antibody-pa1624-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1624-1-WB-anti-kim1-antibody.jpgAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Anti-TIM 1 antibody&#44; PA1624&#44; Western blotting<br>Lane 1: SMMC Cell Lysate <br>Lane 2: HELA Cell Lysate<br>Lane 3: PANC Cell Lysate<br>Lane 4: M231 Cell Lysate<br>Lane 5: M453 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1624-2-IHC-anti-kim1-antibody.jpgAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Anti-TIM 1 antibody&#44; PA1624&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1624-3_1-IF-anti-kim1-antibody.jpgAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Anti-TIM 1 antibody&#44; PA1624&#44; ICC<br>ICC: K562 Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-1-antibody-pa1625-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1625-1-WB-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Anti-Kallikrein 1 antibody&#44; PA1625&#44; Western blotting<br>Lane 1: Recombinant Human KLK1 Protein 10ng <br>Lane 2: Recombinant Human KLK1 Protein 5ng <br>Lane 3: Recombinant Human KLK1 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1625-2-IHC-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Anti-Kallikrein 1 antibody&#44; PA1625&#44; IHC(P)<br>IHC(P): Human Pancreatic Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1625-3.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg; Western blot analysis of KLK1 using anti-KLK1 antibody (PA1625). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat pancreas tissue lysates&#44; <br> Lane 2: mouse pancreas tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLK1 antigen affinity purified polyclonal antibody (Catalog # PA1625) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLK1 at approximately 29KD. The expected band size for KLK1 is at 29KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-2-antibody-pa1626-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1626-1_1-WB-anti-kallikrein-2-antibody.jpgAnti-Kallikrein 2/KLK2 Antibody Picoband&reg; Western blot analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (PA1626). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. <br> Lane 1: human PANC whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 2 antigen affinity purified polyclonal antibody (Catalog # PA1626) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 2 at approximately 29 kDa. The expected band size for Kallikrein 2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1626-2-IHC-anti-kallikrein-2-antibody.jpgAnti-Kallikrein 2/KLK2 Antibody Picoband&reg; IHC analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (PA1626). <br> Kallikrein 2 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kallikrein 2 Antibody (PA1626) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-beta-3-antibody-pa1627-boster.html2026-03-24T05:03:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1627-itgb3-primary-antibodies-wb-testing-1.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband&reg; Western blot analysis of ITGB3 using anti-ITGB3 antibody (PA1627). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human HEL whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: rat spleen tissue lysates,<br> Lane 5: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB3 antigen affinity purified polyclonal antibody (Catalog # PA1627) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGB3 at approximately 100 kDa. The expected band size for ITGB3 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1627-itgb3-primary-antibodies-ihc-testing-2.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband&reg; IHC analysis of ITGB3 using anti-ITGB3 antibody (PA1627). <br> ITGB3 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGB3 Antibody (PA1627) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1627-itgb3-primary-antibodies-ihc-testing-3.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband&reg; IHC analysis of ITGB3 using anti-ITGB3 antibody (PA1627). <br> ITGB3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGB3 Antibody (PA1627) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1627-itgb3-primary-antibodies-ihc-testing-4.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband&reg; IHC analysis of ITGB3 using anti-ITGB3 antibody (PA1627). <br> ITGB3 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGB3 Antibody (PA1627) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1627-itgb3-primary-antibodies-ihc-testing-5.jpgAnti-Integrin beta 3/ITGB3 Antibody Picoband&reg; IHC analysis of ITGB3 using anti-ITGB3 antibody (PA1627). <br> ITGB3 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITGB3 Antibody (PA1627) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-5-antibody-pa1630-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1630-1-WB-anti-klk5-kallikrein-5-antibody.jpgAnti-Kallikrein 5/KLK5 Antibody Picoband&reg;Anti-Kallikrein 5 antibody&#44; PA1630&#44; Western blotting<br>WB: Mouse Liver Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1630-2-IHC-anti-klk5-kallikrein-5-antibody.jpgAnti-Kallikrein 5/KLK5 Antibody Picoband&reg;Anti-Kallikrein 5 antibody&#44; PA1630&#44; IHC(P)<br>IHC(P): Rat Testis Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-11-antibody-pa1631-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1631-1-WB-anti-klk11-kallikrein-11-antibody.jpgAnti-Kallikrein 11/KLK11 Antibody Picoband&reg;Anti-Kallikrein 11 antibody&#44; PA1631&#44; Western blotting<br>All lanes: Anti Kallikrein 11 (PA1631) at 0.5ug/ml<br> Lane 1: U87 Whole Cell Lysate at 40ug<br> Lane 2: A549 Whole Cell Lysate at 40ug<br> Lane 3: HELA Whole Cell Lysate at 40ug<br> Lane 4: MM231 Whole Cell Lysate at 40ug<br> Lane 5: MM453 Whole Cell Lysate at 40ug<br> Lane 6: COLO320 Whole Cell Lysate at 40ug<br> Lane 7: JURKAT Whole Cell Lysate at 40ug<br> Predicted bind size: 31KD<br> Observed bind size: 31KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1631-2-IHC-anti-klk11-kallikrein-11-antibody.jpgAnti-Kallikrein 11/KLK11 Antibody Picoband&reg;Anti-Kallikrein 11 antibody&#44; PA1631&#44; IHC(P)<br>IHC(P): Human Prostatic Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tim-1-antibody-pa1632-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1632-havcr1-primary-antibodies-wb-testing-1.jpgAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Western blot analysis of HAVCR1 using anti-HAVCR1 antibody (PA1632). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAVCR1 antigen affinity purified polyclonal antibody (PA1632) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HAVCR1 at approximately 50 kDa. The expected band size for HAVCR1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1632-havcr1-primary-antibodies-ihc-testing-1.jpgAnti-TIM 1/HAVCR1 Antibody Picoband&reg;IHC analysis of HAVCR1 using anti-HAVCR1 antibody (PA1632). <br>HAVCR1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HAVCR1 Antibody (PA1632) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1632-41420_2023_1432_fig6_html.pngAnti-TIM 1/HAVCR1 Antibody Picoband&reg;The ectopic expression of Sirtuin 6 relieves renal injury and mitochondrial dysfunction upon IRI. A Experimental design. Green arrow showed the injection of pcDNA plasmid or pFlag-Sirtuin 6 overexpression plasmid. Male mice were subjected to IRI or sham respectively, and euthanized 24 h after IRI. B Scr levels in three groups, as indicated. Scr was expressed as milligrams per deciliter. *** P < 0.001 versus sham controls ( n = 5); †† P < 0.01 versus pcDNA group ( n = 5). C Representative western blot of flag tag is shown. D Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. E – G Representative western blot ( E ) and graphical representations of ( F ) PGC-1α and ( G ) TOMM20 protein expression levels are shown. * P < 0.05 versus sham controls ( n = 5); †† P < 0.01, ††† P < 0.001 versus pcDNA group ( n = 5). H Representative micrographs show renal tubular morphologic injury, the expression of KIM-1 and caspase-3, and TUNEL assay in different groups, as indicated. Paraffin sections were subjected to periodic acid–Schiff (PAS) staining, stained with an antibody against KIM-1 and caspase-3. Frozen kidney sections were subjected to TUNEL staining. Arrows indicate positive staining. Scale bar, 50 μm. I – M Representative western blot ( I ) and graphical representations of ( J ) KIM-1, ( K ) FAS-L, ( L ) Bax and ( M ) cleaved caspase-3 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus sham controls ( n = 5); † P < 0.05, †† P < 0.01 versus pcDNA group ( n = 5). N Tubular injury score depending on PAS staining in three groups, as indicated. *** P < 0.001 versus sham controls ( n = 5); ††† P < 0.001 versus pcDNA group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1632-41420_2023_1432_fig5_html.pngAnti-TIM 1/HAVCR1 Antibody Picoband&reg;The ectopic knockdown of AR ameliorates renal injury and mitochondrial dysfunction upon IRI. A Experimental design. Green arrow showed the injection of control-shRNA (pLVX-shRNA) or AR-shRNA (pLVX-shAR) plasmid. Male mice were subjected to IRI or sham respectively, and euthanized 24 h after IRI. B Scr levels in three groups, as indicated. Scr was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls ( n = 5); †† P < 0.01 versus control-shRNA group ( n = 5). C BUN levels in three groups, as indicated. BUN was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls ( n = 5); † P < 0.05 versus control-shRNA group ( n = 5). D , E Representative western blot ( D ) and graphical representations of ( E ) AR protein expression levels are shown. *** P < 0.001 versus sham controls ( n = 5); †† P < 0.01 versus control-shRNA group ( n = 5). F , G Representative western blot ( F ) and graphical representations of ( G ) Sirtuin 6 protein expression levels are shown. ** P < 0.01 versus sham controls ( n = 5); †† P < 0.01 versus control-shRNA group ( n = 5). H Representative micrographs showing the expression of Sirtuin 6 in different groups. Paraffin sections were stained with an antibody against Sirtuin 6. Arrows indicate positive staining. Scale bar, 50 μm. I Representative micrographs show renal tubular morphologic injury, the expression of KIM-1 and caspase-3, and TUNEL assay in different groups, as indicated. Paraffin sections were subjected to PAS staining, stained with an antibody against KIM-1 and caspase-3. Frozen kidney sections were subjected to TUNEL staining. Arrows indicate positive staining. Scale bar, 50 μm. (J – N) Representative western blot ( J ) and graphical representations of ( K ) KIM-1, ( L ) FAS-L, ( M ) Bax and ( N ) cleaved caspase-3 protein expression levels are shown. ** P < 0.01, *** P < 0.001 versus sham controls ( n = 5); †† P < 0.01, ††† P < 0.001 versus control-shRNA group ( n = 5). O Tubular injury scores depending on PAS staining in three groups, as indicated. *** P < 0.001 versus sham controls ( n = 5); ††† P < 0.001 versus control-shRNA group ( n = 5). P Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. Q – S Representative western blot ( Q ) and graphical representations of ( R ) PGC-1α and ( S ) TOMM20 protein expression levels are shown. *** P < 0.001 versus sham controls ( n = 5); ††† P < 0.001 versus control-shRNA group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1632-41420_2023_1432_fig3_html.pngAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Male mice were more susceptive to rhabdomyolysis-induced AKI and tubular apoptosis in kidney. A Experimental design. Female and male mice were intramuscularly injected with 50% glycerol at the dose of 7.5 ml/kg or normal saline respectively. Mice were euthanized 3 days after intramuscular injection. B Scr levels in four groups, as indicated. Scr was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls in male group ( n = 5); †† P < 0.01 versus sham controls in female group ( n = 5); # P < 0.05 versus male group in glycerol group ( n = 5). C Graphical representations show three day-mortality in different genders after glycerol administration, as indicated. * P < 0.05 versus male group (n of male group = 20; n of female group = 17). D Representative micrographs show renal tubular morphologic injury and the expression of KIM-1 in different groups, as indicated. Paraffin sections were subjected to PAS staining and stained with an antibody against KIM-1. Arrows indicate positive staining. Scale bar, 50 μm. E Tubular injury score depending on PAS staining in four groups, as indicated. *** P < 0.001 versus sham controls in male group ( n = 5); ††† P < 0.001 versus sham controls in female group ( n = 5). F – J Representative western blot ( F ) and graphical representations of ( G ) KIM-1, ( H ) FAS-L, ( I ) Bax and ( J ) cleaved caspase-3 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus female group ( n = 5). K Representative micrographs show the expression of caspase-3 and TUNEL staining in different groups, as indicated. Paraffin sections were stained with an antibody against caspase-3. Frozen kidney sections were subjected to TUNEL staining. Arrow indicates positive staining. Scale bar, 50 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1632-41419_2025_7438_fig5_html.pngAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Ectopic CD44 aggravates tubular cell apoptosis and kidney injury in IRI mice. A Experimental design: Green arrow indicated the injection of pcDNA3 plasmid or p-HA-CD44 overexpression plasmid. Mice were subjected to IRI surgery or sham surgery, respectively, as shown in the red arrow. Mice are euthanized 24 h after surgery. B Scr levels in three groups, as indicated. Scr was expressed as milligrams per deciliter. * P < 0.05 versus sham group; # P < 0.05 versus IRI group injected with pcDNA3 ( n = 5). C BUN levels in three groups, as indicated. BUN was expressed as milligrams per deciliter. ** P < 0.01 versus sham group; # P < 0.05 versus IRI group injected with pcDNA3 ( n = 5). D and E Representative western blot of CD44 ( D ) and graphical presentations ( E ) of protein levels are shown. ** P < 0.01 versus sham group; # P < 0.05 versus IRI group injected with pcDNA3 ( n = 5). F Representative micrographs show the expression of CD44, and TUNEL assay in different groups, as indicated. Arrows indicate positive staining. Frozen kidney sections were subjected to TUNEL assay or stained with an antibody against CD44. Scale bar, 50 μm. G–J Representative western blot ( G ) and graphical presentations of H BCL2, I BAX, and J cleaved caspase 3 protein expression levels are shown. * P < 0.05, ** P < 0.01 versus sham group; # P < 0.05, ### P < 0.001 versus IRI group injected with pcDNA3 ( n = 5). K and L Representative western blot of NGAL ( K ) and graphical presentations ( L ) of protein levels are shown. ** P < 0.01 versus sham group; ## P < 0.01 versus IRI group injected with pcDNA3 ( n = 5). M Representative micrographs show renal tubular morphologic injury and the expression of KIM-1 in different groups, as indicated. Paraffin sections were subjected to PAS staining, and were stained with an antibody against KIM-1. Arrows indicate positive staining. Scale bar, 50 μm. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-025-07438-x'>39979265</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1632-41420_2023_1432_fig1_html.pngAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Male mice were more susceptive to IRI and tubular apoptosis in kidney. A Experimental design. Female and male mice were subjected to IRI or sham respectively, and euthanized 24 h after IRI. B Scr levels in four groups, as indicated. Scr was expressed as milligrams per deciliter. ** P < 0.01 versus sham controls in male group ( n = 5). C BUN levels in four groups, as indicated. BUN was expressed as milligrams per deciliter. * P < 0.05 versus sham controls in male group ( n = 5). D Representative micrographs show renal tubular morphologic injury, the expression of KIM-1 and caspase-3, and TUNEL assay in different groups, as indicated. Paraffin sections were subjected to periodic acid–Schiff (PAS) staining, stained with an antibody against KIM-1 and caspase-3. Frozen kidney sections were subjected to TUNEL assay. Arrows indicate positive staining. Scale bar, 50 μm. E – I Representative western blot ( E ) and graphical representations of ( F ) FAS-L, ( G ) Bax, ( H ) cleaved caspase-3 and ( I ) KIM-1 protein expression levels are shown. * P < 0.05, ** P < 0.01 versus male group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-1-antibody-pa1633-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1633-1_1-WB-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/Ngfg Antibody Picoband&reg; Western blot analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (PA1633). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat pancreas tissue lysates,<br> Lane 2: rat kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 1 antigen affinity purified polyclonal antibody (Catalog # PA1633) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 1 at approximately 29 kDa. The expected band size for Kallikrein 1 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1633-2-IHC-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/Ngfg Antibody Picoband&reg; IHC analysis of Kallikrein 1 using anti-Kallikrein 1 antibody (PA1633). <br>Kallikrein 1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Kallikrein 1 Antibody (PA1633) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mrp1-antibody-pa1634-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1634-1-WB-anti-mrp1-antibody.jpgAnti-MRP1/ABCC1 Antibody Picoband&reg;Anti-MRP1 antibody&#44; PA1634&#44; Western blotting<br>Lane 1: JURKAT Cell Lysate <br>Lane 2: CEM Cell Lysate <br>Lane 3: A549 Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1634-2-IHC-anti-mrp1-antibody.jpgAnti-MRP1/ABCC1 Antibody Picoband&reg;Anti-MRP1 antibody&#44; PA1634&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lamin-b2-antibody-pa1636-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1636-lamin-b2-primary-antibodies-wb-testing-1.jpgAnti-Lamin B2/LMNB2 Antibody Picoband&reg; Western blot analysis of Lamin B2 using anti-Lamin B2 antibody (PA1636). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Hela (nucleus) lysates, <br> Lane 3: human K562 (nucleus) lysates, <br> Lane 4: human PC-3 whole cell lysates, <br> Lane 5: rat C6 whole cell lysates, <br> Lane 6: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B2 antigen affinity purified polyclonal antibody (Catalog # PA1636) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B2 at approximately 70 kDa. The expected band size for Lamin B2 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1636-2-IHC-anti-lamin-b2-antibody.jpgAnti-Lamin B2/LMNB2 Antibody Picoband&reg; IHC analysis of Lamin B2 using anti-Lamin B2 antibody (PA1636). <br> Lamin B2 was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lamin B2 Antibody (PA1636) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1636-lmnb2-primary-antibodies-ihc-testing-4.jpgAnti-Lamin B2/LMNB2 Antibody Picoband&reg;IHC analysis of Lamin B2/LMNB2 using anti-Lamin B2/LMNB2 antibody (PA1636). <br>Lamin B2/LMNB2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B2/LMNB2 Antibody (PA1636) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1636-3.jpgAnti-Lamin B2/LMNB2 Antibody Picoband&reg; IHC analysis of Lamin B2 using anti-Lamin B2 antibody (PA1636).<br> Lamin B2 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lamin B2 Antibody (PA1636) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nrg2-antibody-pa1637-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1637-1-WB-anti-nrg2-antibody.jpgAnti-NRG2 Antibody Picoband&reg;Anti-NRG2 antibody&#44; PA1637&#44; Western blotting<br>All lanes: Anti NRG2 (PA1637) at 0.5ug/ml<br> Lane 1: Rat Liver Tissue Lysate at 50ug<br> Lane 2: COLO320 Whole Cell Lysate at 40ug<br> Lane 3: SMMC Whole Cell Lysate at 40ug<br> Lane 4: SW620 Whole Cell Lysate at 40ug<br> Predicted bind size: 91KD<br> Observed bind size: 91KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallistatin-antibody-pa1638-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1638-1-IHC-anti-kallistatin-serpina4-antibody.jpgAnti-Kallistatin/SERPINA4 Antibody Picoband&reg;Anti-Kallistatin antibody&#44; PA1638&#44; IHC(P)<br>IHC(P): Human Liver Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1638-2-WB-anti-kallistatin-serpina4-antibody.jpgAnti-Kallistatin/SERPINA4 Antibody Picoband&reg;Anti-Kallistatin antibody&#44; PA1638&#44; Western blotting<br>Lane 1: SMMC Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1638-3-WB-anti-kallistatin-serpina4-antibody.jpgAnti-Kallistatin/SERPINA4 Antibody Picoband&reg;Anti-Kallistatin antibody&#44; PA1638&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: SKOV Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xrcc1-antibody-pa1639-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1639-xrcc1-primary-antibodies-wb-testing-1.jpgAnti-XRCC1 Antibody Picoband&reg; Western blot analysis of XRCC1 using anti-XRCC1 antibody (PA1639). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC1 antigen affinity purified polyclonal antibody (Catalog # PA1639) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC1 at approximately 90 kDa. The expected band size for XRCC1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1639-2-IHC-anti-xrcc1-antibody.jpgAnti-XRCC1 Antibody Picoband&reg; IHC analysis of XRCC1 using anti-XRCC1 antibody (PA1639). <br> XRCC1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XRCC1 Antibody (PA1639) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1639-3-IF-anti-xrcc1-antibody.jpgAnti-XRCC1 Antibody Picoband&reg; ICC analysis of XRCC1 using anti-XRCC1 antibody (PA1639). <br> XRCC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-XRCC1 Antibody (PA1639) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1639-xrcc1-primary-antibodies-if-testing-4.jpgAnti-XRCC1 Antibody Picoband&reg; IF analysis of XRCC1 using anti-XRCC1 antibody (PA1639) and anti-Beta Tubulin antibody (M01857-3).<br> XRCC1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/mL rabbit anti-XRCC1 Antibody (PA1639) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ku80-antibody-pa1641-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-xrcc5-primary-antibodies-wb-testing-1.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; Western blot analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human 293T whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC5 antigen affinity purified polyclonal antibody (Catalog # PA1641) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC5 at approximately 83 kDa. The expected band size for XRCC5 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-2_1.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IHC analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> XRCC5 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-XRCC5 Antibody (PA1641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1641-3-IF-anti-ku80-antibody.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; ICC analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> XRCC5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-XRCC5 Antibody (PA1641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-4.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IHC analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> XRCC5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-XRCC5 Antibody (PA1641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-5.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IHC analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> XRCC5 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-XRCC5 Antibody (PA1641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-6.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IHC analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> XRCC5 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-XRCC5 Antibody (PA1641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-7.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IF analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> XRCC5 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-XRCC5 Antibody (PA1641) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-8.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IF analysis of XRCC5 using anti-XRCC5 antibody (PA1641). <br> XRCC5 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-XRCC5 Antibody (PA1641) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1641-9.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-XRCC5 antibody (PA1641).<br>Overlay histogram showing SiHa cells stained with PA1641 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XRCC5 Antibody (PA1641,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ku70-antibody-pa1642-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-xrcc6-primary-antibodies-wb-testing-1_1.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; Western blot analysis of XRCC6 using anti-XRCC6 antibody (PA1642). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human SiHa whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: human CACO-2 whole cell lysates,<br> Lane 6: human Hela whole cell lysates,<br> Lane 7: human U2OS whole cell lysates,<br> Lane 8: human RT4 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC6 antigen affinity purified polyclonal antibody (Catalog # PA1642) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC6 at approximately 70 kDa. The expected band size for XRCC6 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-2_2.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of XRCC6 using anti-XRCC6 antibody (PA1642). <br> XRCC6 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XRCC6 Antibody (PA1642) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1642-3-IF-anti-ku70-antibody.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; ICC analysis of XRCC6 using anti-XRCC6 antibody (PA1642). <br> XRCC6 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-XRCC6 Antibody (PA1642) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1642-4-IF-anti-ku70-antibody.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; ICC analysis of XRCC6 using anti-XRCC6 antibody (PA1642). <br> XRCC6 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-XRCC6 Antibody (PA1642) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-xrcc6-primary-antibodies-if-testing-5.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IF analysis of XRCC6 using anti-XRCC6 antibody (PA1642). <br> XRCC6 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-XRCC6 Antibody (PA1642) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-xrcc6-primary-antibodies-fcm-testing-6.pngAnti-Ku70/XRCC6 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-XRCC6 antibody (PA1642). <br>Overlay histogram showing MCF-7 cells stained with PA1642 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XRCC6 Antibody (PA1642, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angiotensinogen-antibody-pa1643-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1643-agt-primary-antibodies-wb-testing-1_1.jpgAnti-Angiotensinogen/AGT Antibody Picoband&reg; Western blot analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody (PA1643). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates, <br> Lane 2: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiotensinogen/AGT antigen affinity purified polyclonal antibody (Catalog # PA1643) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiotensinogen/AGT at approximately 55 kDa. The expected band size for Angiotensinogen/AGT is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1643-agt-primary-antibodies-ihc-testing-2.jpgAnti-Angiotensinogen/AGT Antibody Picoband&reg; IHC analysis of Angiotensinogen/AGT using anti-Angiotensinogen/AGT antibody (PA1643). <br> Angiotensinogen/AGT was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Angiotensinogen/AGT Antibody (PA1643) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alox5-antibody-pa1644-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1644-1-WB-anti-alox5-5-lo-antibody.jpgAnti-5 Lipoxygenase/ALOX5 Antibody Picoband&reg;Anti-ALOX5 antibody&#44; PA1644&#44; Western blotting<br>All lanes: Anti ALOX5 (PA1644) at 0.5ug/ml<br> Lane 1: SW620 Whole Cell Lysate at 40ug<br> Lane 2: JURKAT Whole Cell Lysate at 40ug<br> Lane 3: COLO320 Whole Cell Lysate at 40ug<br> Lane 4: A549 Whole Cell Lysate at 40ug<br> Lane 5: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 78KD<br> Observed bind size: 78KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1644-2-IHC-anti-alox5-5-lo-antibody.jpgAnti-5 Lipoxygenase/ALOX5 Antibody Picoband&reg;Anti-ALOX5 antibody&#44; PA1644&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bub3-antibody-pa1645-boster.html2026-03-24T05:03:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1645-1-WB-anti-bub3-antibody.jpgAnti-Bub3 Antibody Picoband&reg;Anti-Bub3 antibody&#44; PA1645&#44; Western blotting<br>All lanes: Anti Bub3 (PA1645) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Lane 4: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 37KD<br>Observed bind size: 37KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1645-2-IHC-anti-bub3-antibody.jpgAnti-Bub3 Antibody Picoband&reg;Anti-Bub3 antibody&#44; PA1645&#44; IHC(P)<br>IHC(P): Rat Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1645-3-IHC-anti-bub3-antibody.jpgAnti-Bub3 Antibody Picoband&reg;Anti-Bub3 antibody&#44; PA1645&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1645-4-IF-anti-bub3-antibody.jpgAnti-Bub3 Antibody Picoband&reg;Anti-Bub3 antibody&#44; PA1645&#44; ICC<br>ICC: A549 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1645-5-IF-anti-bub3-antibody.jpgAnti-Bub3 Antibody Picoband&reg;Anti-Bub3 antibody&#44; PA1645&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1645-6.jpgAnti-Bub3 Antibody Picoband&reg; IHC analysis of BUB3 using anti-BUB3 antibody (PA1645).<br> BUB3 was detected in frozen section of rat lung tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BUB3 Antibody (PA1645) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-5ht1a-receptor-antibody-pa1647-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1647-htr1a-primary-antibodies-wb-testing-1.jpgAnti-5HT1A Receptor/HTR1A Antibody Picoband&reg; Western blot analysis of HTR1A using anti-HTR1A antibody (PA1647). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: rat liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HTR1A antigen affinity purified polyclonal antibody (Catalog # PA1647) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HTR1A at approximately 46 kDa. The expected band size for HTR1A is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1647-elife-94765-fig8-v1.jpgAnti-5HT1A Receptor/HTR1A Antibody Picoband&reg;VLZ stimulates megakaryocytopoiesis via the 5-HT1A receptor. (a) Representative immunoblot images and biochemical quantification of 5-HTR1A after treatment with VLZ (2.5, 5, and 10 μM) in Meg-01 cells for 5 days (n=3 per group). (b) The DARTS assay for target validation. 5-HTR1A protein stability was increased upon VLZ (200 μM) treatment in Meg-01 lysates. Pronase was added using several dilutions (1:500, 1:1000, or 1500) from 50 μg/mL stock for 10 min at 40 °C (n=3 per group). (c) The DARTS assay demonstrated the dose-dependent binding of VLZ to 5-HTR1A in Meg-01 cells. Treatment with pronase (1:1000) was conducted for 10 min at 40 °C (n=3 per group). (d) Immunofluorescence analysis of the expression of 5-HTR1A in Meg-01 cells after VLZ (2.5, 5, and 10 μM) intervention for 5 days. Cells were stained with DAPI for nuclei (blue) and antibodies for 5-HTR1A (green). Bars represent 100 μm. (e–k) Meg-01 cells were treated with VLZ (10 μM), WAY-100635 (2.5 μM), VLZ (10 μM)+WAY-100635 (2.5 μM) for 5 days. (e) Representative images, bars represent 25 μm. (f) Giemsa staining of Meg-01 cells, bars represent 100 μm. (g) Phalloidin staining of Meg-01 cells, bars represent 100 μm. (h, i) Flow cytometry analysis of the expression of CD41/CD42b and the DNA ploidy. (j, k) The histogram shows the percentage of CD41+/CD42b+ cells and DNA ploidy for each group. (l) Western blot analysis of 5-HTR1A, RAS and ERK expression after Meg-01 cells were treated with VLZ (10 μM), WAY-100635 (2.5 μM), and VLZ (10 μM)+WAY-100635 (2.5 μM) for 5 days. The histogram shows the expression of 5-HTR1A, RAS, and ERK in each group (n=3 per group). The data represent the mean ± SD of three independent experiments. *p≤0.05, **p≤0.01, and ***p≤0.001, ns: no significance, vs the control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://elifesciences.org/articles/94765'>38573820</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1647-htr1a-primary-antibodies-ihc-testing-2.jpgAnti-5HT1A Receptor/HTR1A Antibody Picoband&reg; IHC analysis of HTR1A using anti-HTR1A antibody (PA1647). <br> HTR1A was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1647-htr1a-primary-antibodies-ihc-testing-3.jpgAnti-5HT1A Receptor/HTR1A Antibody Picoband&reg; IHC analysis of HTR1A using anti-HTR1A antibody (PA1647). <br> HTR1A was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1647-htr1a-primary-antibodies-ihc-testing-4.jpgAnti-5HT1A Receptor/HTR1A Antibody Picoband&reg; IHC analysis of HTR1A using anti-HTR1A antibody (PA1647). <br> HTR1A was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR1A Antibody (PA1647) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-maoa-antibody-pa1648-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1648-maoa-primary-antibodies-wb-testing-1.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; Western blot analysis of MAOA using anti-MAOA antibody (PA1648). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates, <br> Lane 2: human placenta tissue lysates, <br> Lane 3: rat heart tissue lysates, <br> Lane 4: rat liver tissue lysates, <br> Lane 5: rat kidney tissue lysates, <br> Lane 6: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOA antigen affinity purified polyclonal antibody (Catalog # PA1648) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAOA at approximately 65 kDa. The expected band size for MAOA is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1648-2-IHC-anti-maoa-monoamine-oxidase-a-antibody.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg;Anti-MAOA antibody&#44; PA1648&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-maob-antibody-pa1649-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1649-2-IHC-anti-maob-antibody.jpgAnti-Monoamine Oxidase B/MAOB Antibody Picoband&reg;Anti-MAOB antibody&#44; PA1649&#44; IHC(P)<br>IHC(P): Rat Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1649-1_1-WB-anti-maob-antibody.jpgAnti-Monoamine Oxidase B/MAOB Antibody Picoband&reg;Anti-MAOB antibody&#44; PA1649&#44; Western blotting<br>All lanes: Anti MAOB (PA1649) at 0.5ug/ml<br> Lane 1: Mouse Liver Tissue Lysate at 50ug<br> Lane 2: Mouse Lung Tissue Lysate at 50ug <br> Lane 3: Rat Kidney Tissue Lysate at 50ug<br> Lane 4: Rat Brain Tissue Lysate at 50ug<br> Lane 5: Rat Liver Tissue Lysate at 50ug<br> Lane 6: Rat Lung Tissue Lysate at 50ug<br> Predicted bind size: 59KD<br> Observed bind size: 59KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm2-antibody-pa1650-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/A/PA1650-MCM2-primary-antibodies-WB-testing-1.jpgAnti-MCM2 Antibody Picoband&reg; Western blot analysis of MCM2 using anti-MCM2 antibody (PA1650). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse spleen tissue lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM2 antigen affinity purified polyclonal antibody (Catalog # PA1650) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM2 at approximately 125 kDa. The expected band size for MCM2 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1650-2-IHC-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1650&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1650-3-IHC-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1650&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1650-4-IF-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1650&#44; ICC<br>ICC: A549 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1650-mcm2-primary-antibodies-if-testing-5.jpgAnti-MCM2 Antibody Picoband&reg; IF analysis of MCM2 using anti-MCM2 antibody (PA1650). <br> MCM2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM2 Antibody (PA1650) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1650-mcm2-primary-antibodies-fcm-testing-6.pngAnti-MCM2 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-MCM2 antibody (PA1650). <br>Overlay histogram showing HL-60 cells stained with PA1650 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM2 Antibody (PA1650, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm3-antibody-pa1651-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g001.jpgAnti-MCM3 Antibody Picoband&reg;Expression patterns of MCM3 in pan-cancer. (A) Differences in MCM3 between tumor and normal tissues based on TCGA and GETx data. (B) Comparison of protein levels based on CPTAC data. (C) Clinical correlation analysis based on GEPIA database. (D) The genomic alteration of MCM3 in pan-cancer. (E) Immunofluorescence results showed the localization of MCM3 in cell lines. **** p < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g002.jpgAnti-MCM3 Antibody Picoband&reg;Prognostic value of MCM3 in pan-cancer. (A) The heatmap shows results of univariate Cox regression analysis. (B) Forest plot of MCM3 expression and OS across cancers. (C) Forest plot of MCM3 expression and PFI across cancers. (D) Forest plot of MCM3 expression and DSS across cancers. (E) Forest plot of MCM3 expression and DFI across cancers.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g003.jpgAnti-MCM3 Antibody Picoband&reg;Relationship between MCM expression and immune-related features in pan-cancer. (A) MCM3 expression and tumor microenvironment relate parameters. (B) MCM3 expression and immune cell infiltration. (C) MCM3 expression and immune checkpoints. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g004.jpgAnti-MCM3 Antibody Picoband&reg;Immunotherapy and drug sensitivity analysis. (A) Relationship between MCM3 expression and TMB, MSI, and neoantigens. (B–E) Prognostic significance of MCM3 and proportion of immunotherapy response between high- and low-MCM3 groups in four cohorts receiving ICB therapy. (F) Drug sensitivity analysis of MCM3 based on CTPR and GDSC data. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g005.jpgAnti-MCM3 Antibody Picoband&reg;Single-cell analysis of MCM3. (A) Correlation between MCM3 and 14 biological functions. (B) The top three functions in BRCA, LUAD, MEL and glioma. (C) Datasets of single-cell expression of MCM3 from TISCH website. (D) Distribution of MCM3 among cell types in the GSE111360 and GSE140228 datasets. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g006.jpgAnti-MCM3 Antibody Picoband&reg;Correlation between MCM3 expression and clinical features and prognosis of LGG. (A, B) Relationship between MCM3 and clinical features in TCGA and CGGA cohorts. (C, D) Evaluation of the ability of MCM3 expression to predict prognosis in TCGA and CGGA cohorts. ns: no significance, ** p < 0.01, *** p < 0.001, **** p < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g007.jpgAnti-MCM3 Antibody Picoband&reg;Construction of a prognostic nomogram and analysis of MCM3 related functions in LGG. (A) Univariate/multivariate Cox analysis was performed based on TCGA cohort. (B) Establishment of a prognostic nomogram based on multivariate analysis results. (C–F) Nomogram model evaluation, including timeROC, calibration, DCA and KM curves. (G) GO and KEGG analyses based on differentially expressed genes in TCGA. (H) An interaction network between GO and KEGG. (I) The GSEA analysis in TCGA cohort. (J) The waterfall map shows the top 10 genes with the highest mutation probability. (K, L) TMB and TIDE score were compared between the two groups. ** p < 0.01, **** p < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1651-fphar-15-1390615-g008.jpgAnti-MCM3 Antibody Picoband&reg;Validation of the association between MCM3 and clinical features and prognosis of LGG. (A) MCM3 protein expression in normal and tumor tissues from HPA database. (B) Representative plots of negative and positive immunohistochemical results. (C, D) Relationship between MCM3 expression and tumor size and grade. (E, F) Survival curves for OS and PFS in clinical cohort. (G, H) Univariate and multivariate Cox regression analysis for OS and PFS.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1390615/full'>38698811</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-mcm3-primary-antibodies-wb-testing-1.jpgAnti-MCM3 Antibody Picoband&reg; Western blot analysis of MCM3 using anti-MCM3 antibody (PA1651). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: rat thymus tissue lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse thymus tissue lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM3 antigen affinity purified polyclonal antibody (PA1651) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MCM3 at approximately 100-110 kDa. The expected band size for MCM3 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-mcm3-primary-antibodies-ihc-testing-2.jpgAnti-MCM3 Antibody Picoband&reg; IHC analysis of MCM3 using anti-MCM3 antibody (PA1651). <br> MCM3 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCM3 Antibody (PA1651) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-mcm3-primary-antibodies-ihc-testing-3.jpgAnti-MCM3 Antibody Picoband&reg; IHC analysis of MCM3 using anti-MCM3 antibody (PA1651). <br> MCM3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCM3 Antibody (PA1651) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-mcm3-primary-antibodies-ihc-testing-4.jpgAnti-MCM3 Antibody Picoband&reg; IHC analysis of MCM3 using anti-MCM3 antibody (PA1651). <br> MCM3 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCM3 Antibody (PA1651) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-mcm3-primary-antibodies-if-testing-5.jpgAnti-MCM3 Antibody Picoband&reg; IF analysis of MCM3 using anti-MCM3 antibody (PA1651) and anti-Tubulin Alpha antibody (M03989-3).<br> MCM3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MCM3 Antibody (PA1651) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1642-mcm3-primary-antibodies-fcm-testing-6.jpgAnti-MCM3 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-MCM3 antibody (PA1651). <br> Overlay histogram showing Hela cells stained with PA1651 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM3 Antibody (PA1651, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm5-antibody-pa1653-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1653-1-WB-anti-mcm5-antibody.jpgAnti-MCM5 Antibody Picoband&reg;Anti-MCM5 antibody&#44; PA1653&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate <br>Lane 2: Rat Brain Tissue Lysate Lane3: JURKAT Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1653-2-IHC-anti-mcm5-antibody.jpgAnti-MCM5 Antibody Picoband&reg;Anti-MCM5 antibody&#44; PA1653&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1653-3.jpgAnti-MCM5 Antibody Picoband&reg; Western blot analysis of MCM5 using anti-MCM5 antibody (PA1653). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM5 antigen affinity purified polyclonal antibody (Catalog # PA1653) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM5 at approximately 82KD. The expected band size for MCM5 is at 82KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1653-mcm5-primary-antibodies-if-testing-4.jpgAnti-MCM5 Antibody Picoband&reg; IF analysis of MCM5 using anti-MCM5 antibody (PA1653). <br> MCM5 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM5 Antibody (PA1653) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1653-mcm5-primary-antibodies-fcm-testing-5.pngAnti-MCM5 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-MCM5 antibody (PA1653). <br>Overlay histogram showing HL-60 cells stained with PA1653 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM5 Antibody (PA1653, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aplp2-antibody-pa1654-1-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1654-1-aplp2-primary-antibodies-wb-testing-1.jpgAnti-Amyloid-like protein 2 APLP2 Antibody Picoband&reg; Western blot analysis of APLP2 using anti-APLP2 antibody (PA1654-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP2 antigen affinity purified polyclonal antibody (Catalog # PA1654-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APLP2 at approximately 100-110 kDa. The expected band size for APLP2 is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytoglobin-antibody-pa1657-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1657-1-WB-anti-cytoglobin-antibody.jpgAnti-Cytoglobin/CYGB Antibody Picoband&reg;Anti-Cytoglobin antibody&#44; PA1657&#44; Western blotting<br>All lanes: Anti Cytoglobin (PA1657) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 50ug<br> Lane 2: Rat Small Intestine Tissue Lysate at 50ug<br> Lane 3: Rat Liver Tissue Lysate at 50ug<br> Lane 4: Rat Kidney Tissue Lysate at 50ug<br> Lane 5: SGC Whole Cell Lysate at 40ug<br> Lane 6: COLO320 Whole Cell Lysate at 40ug<br> Lane 7: SMMC Whole Cell Lysate at 40ug<br> Lane 8: PANC Whole Cell Lysate at 40ug<br> Lane 9: HELA Whole Cell Lysate at 40ug<br> Predicted bind size: 21KD<br> Observed bind size: 21KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lcn1-antibody-pa1659-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1659-1-WB-anti-lcn1-lipocalin-1-antibody.jpgAnti-Lipocalin-1 LCN1 Antibody Picoband&reg;Anti-LCN1 antibody&#44; PA1659&#44; Western blotting<br>All lanes: Anti LCN1 (PA1659) at 0.5ug/ml<br> Lane 1: JURKAT Whole Cell Lysate at 40ug<br> Lane 2: COLO320 Whole Cell Lysate at 40ug<br> Lane 3: SCG Whole Cell Lysate at 40ug<br> Lane 4: HELA Whole Cell Lysate at 40ug<br> Predicted bind size: 19KD<br> Observed bind size: 19KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1659-2-IHC-anti-lcn1-lipocalin-1-antibody.jpgAnti-Lipocalin-1 LCN1 Antibody Picoband&reg;Anti-LCN1 antibody&#44; PA1659&#44;IHC(P)<br>IHC(P): Human Prostatic Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myd88-antibody-pa1660-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1660-myd88-primary-antibodies-wb-testing-1.jpgAnti-MyD88 Antibody Picoband&reg;Western blot analysis of MYD88 using anti-MYD88 antibody (PA1660). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human MOLT-4 whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (PA1660) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MYD88 at approximately 36 kDa. The expected band size for MYD88 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1660-myd88-primary-antibodies-ihc-testing-1.jpgAnti-MyD88 Antibody Picoband&reg;IHC analysis of MYD88 using anti-MYD88 antibody (PA1660). <br>MYD88 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYD88 Antibody (PA1660) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1660-myd88-primary-antibodies-ihc-testing-2.jpgAnti-MyD88 Antibody Picoband&reg;IHC analysis of MYD88 using anti-MYD88 antibody (PA1660). <br>MYD88 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYD88 Antibody (PA1660) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1660-myd88-primary-antibodies-ip-testing-1.jpgAnti-MyD88 Antibody Picoband&reg;Immunoprecipitating (IP) MYD88 in HepG2 whole cell lysate.<br> Western blot analysis of MYD88 using anti-MYD88 antibody (PA1660); <br> Lane 1: HepG2 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-MYD88 antibody in HepG2 whole cell lysate;<br> Lane 3: anti-MYD88 antibody (2μg) + HepG2 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MYD88 antigen affinity purified polyclonal antibody (PA1660) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MYD88 at approximately 36 kDa. The expected band size for MYD88 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1660-fphar-15-1447560-g006.jpgAnti-MyD88 Antibody Picoband&reg;HBOA inhibits the TLR4/NF-κB signaling pathway. The protein expressions of (A) TLR4 and MyD88, (B) p-IKKα/β, IKKα/β, p-IκBα, IκBα, p-NF-κBp65, and NF-κBp65, and (C) nucleus NF-κBp65, Lamin B1, and cytosol NF-κBp65 were examined by Western blotting. * P < 0.05, ** P < 0.01 versus the model group; ## P < 0.01 versus the normal group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1447560/full'>39323644</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ndufa1-antibody-pa1661-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1661-ndufa1-primary-antibodies-wb-testing-1.jpgAnti-NDUFA1 Antibody Picoband&reg; Western blot analysis of NDUFA1 using anti-NDUFA1 antibody (PA1661). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: rat heart tissue lysates,<br> Lane 3: rat kidney tissue lysates, <br> Lane 4: mouse heart tissue lysates, <br> Lane 5: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA1 antigen affinity purified polyclonal antibody (Catalog # PA1661) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA1 at approximately 8 kDa. The expected band size for NDUFA1 is at 8 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1661-ndufa1-primary-antibodies-ihc-testing-2_1.jpgAnti-NDUFA1 Antibody Picoband&reg; IHC analysis of NDUFA1 using anti-NDUFA1 antibody (PA1661). <br> NDUFA1 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA1 Antibody (PA1661) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1661-ndufa1-primary-antibodies-ihc-testing-3.jpgAnti-NDUFA1 Antibody Picoband&reg; IHC analysis of NDUFA1 using anti-NDUFA1 antibody (PA1661). <br> NDUFA1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA1 Antibody (PA1661) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1661-ndufa1-primary-antibodies-ihc-testing-4_1.jpgAnti-NDUFA1 Antibody Picoband&reg; IHC analysis of NDUFA1 using anti-NDUFA1 antibody (PA1661). <br> NDUFA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDUFA1 Antibody (PA1661) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1661-ndufa1-primary-antibodies-ihc-testing-5_1.jpgAnti-NDUFA1 Antibody Picoband&reg; IHC analysis of NDUFA1 using anti-NDUFA1 antibody (PA1661). <br> NDUFA1 was detected in a frozen section of Rat Cardiac Muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NDUFA1 Antibody (PA1661) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1661-ndufa1-primary-antibodies-if-testing-6.jpgAnti-NDUFA1 Antibody Picoband&reg; IF analysis of NDUFA1 using anti-NDUFA1 antibody (PA1661).<br> NDUFA1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDUFA1 Antibody (PA1661) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1661-ndufa1-primary-antibodies-fcm-testing-7.jpgAnti-NDUFA1 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-NDUFA1 antibody (PA1661). <br> Overlay histogram showing HepG2 cells stained with PA1661 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFA1 Antibody (PA1661, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-netrin-1-antibody-pa1662-boster.html2026-03-24T05:03:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1662-1-WB-anti-netrin-1-antibody.jpgAnti-Netrin 1/NTN1 Antibody Picoband&reg;Anti-Netrin 1 antibody&#44; PA1662&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cias1-nalp3-antibody-pa1665-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-nlrp3-primary-antibodies-wb-testing-1_1.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg; Western blot analysis of NLRP3 using anti-NLRP3 antibody (PA1665). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates, <br> Lane 2: mouse thyus tissue lysates, <br> Lane 3: mouse RAW264.7 whole cell lysates, <br> Lane 4: mouse J774A.1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-nlrp3-primary-antibodies-wb-testing-2.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg; Western blot analysis of NLRP3 using anti-NLRP3 antibody (PA1665). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1:human THP-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP3 antigen affinity purified polyclonal antibody (PA1665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NLRP3 at approximately 110 kDa. The expected band size for NLRP3 is at 118kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-nlrp3-primary-antibodies-ihc-testing-3_1.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg; IHC analysis of NLRP3 using anti-NLRP3 antibody (PA1665). <br> NLRP3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NLRP3 Antibody (PA1665) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-nlrp3-primary-antibodies-fcm-testing-4.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-NLRP3 antibody (PA1665). <br> Overlay histogram showing THP-1 cells stained with PA1665 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NLRP3 Antibody (PA1665, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12964_2019_406_fig6_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;High-salt-elevated NFAT5 mediates transcription of NLRP3 and IL-1β in ECs. a-b mRNA and protein levels of NLRP3 in ECs treated with Adenovirus-null (Ad-null, 5 MOI) and Adenovirus-NFAT5 (Ad-NFAT5, 2 MOI or 5 MOI). c-d High-salt increases binding of NFAT5 to the promoter region of IL-1β. Diagram showing the region of the NFAT5 binding site upstream of the transcription start site (TSS) of NLRP3, and the regions that were used to analyze NFAT5 binding by ChIP. ChIP results are relative to 270 mosmol/kg. e-f mRNA and protein levels of NLRP3 in ECs treated with high-salt and transfected with Ctr siRNA or NFAT5 siRNA. g Protein secretion of IL-1β in ECs treated with high-salt and transfected with Ctr siRNA or NFAT5 siRNA. h High-salt increases binding of NFAT5 to the promoter region of IL-1β. i Protein levels of pro-IL-1β in ECs treated with high-salt and transfected with Ctr siRNA or NFAT5 siRNA. All data were presented as mean ± SEM, N ≥ 3. * p < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12964-019-0406-7'>31429763</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12964_2019_406_fig1_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;High-salt intake predisposes atherosclerosis and upregulates NLRP3 expression. a Schematic shows the process of atherosclerosis formation. b Oil Red O staining of aortas and quantification of percentage lesion areas in the thoracic aorta (TA) and aortic arch (AA) of ApoE −/− mice ( n = 10) fed with a normal or high-salt diet for 12 weeks. c Immunohistochemistry staining for NLRP3 and CD31 in the kidney at 4 weeks. Nuclei, hematoxylin staining. d En face immunofluorescent staining of NLRP3 of ECs in TA and AA of ApoE−/− mice fed with a normal or high-salt diet for 4 weeks. See Additional file : Figure S1D for NLRP3 mRNA qPCR. All data were presented as mean ± SEM, N ≥ 9. * p < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12964-019-0406-7'>31429763</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12964_2019_406_fig3_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;High-salt elevates endothelial inflammation via NLRP3. a-d Quantification of mRNA levels of E-selectin, ICAM-1, VCAM-1 and MCP-1 in TA and AA of ApoE−/− mice fed with a normal or high-salt diet. e-h Quantification of mRNA levels of E-selectin, VCAM-1, ICAM-1, and MCP-1 in ECs treated with high-salt and transfected with Control siRNA or NLRP3 siRNA. i, j The adhesion of Calcein-labeled THP-1 monocytes in ECs treated with high-salt and transfected with Control siRNA or NLRP3 siRNA. All data were presented as mean ± SEM, N ≥ 3. * p < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12964-019-0406-7'>31429763</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_3389_fig1_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;NLRP3 and cleaved-caspase-1 are upregulated in mice heart 3 days after CME intervention. A Representative immunoblots of NLRP3 and cleaved-caspase-1 expression in sham and CME mice hearts. B Densitometric analysis of relative protein expressions, Vinculin is used as loading control. n = 4 per group. Data represent the mean ± SEM of three replicates. C Representative images of immunofluorescent double staining of Troponin T and NLRP3 in cardiac tissues. Scale bars = 50 µm. n = 4 per group. ∗ P < 0.05, ∗ ∗ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-03389-1'>33436548</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_3389_fig3_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;RVS restrains the activation of the NLRP3 inflammasome at 3 days after CME intervention. A Representative immunoblots for myocardial NLRP3, caspase-1, cleaved-caspase-1, IL-1β, GSDMD, GSDMD-N in the hearts of sham, CME and RVS-treated mice. B The densitometric analysis of relative protein expressions, GAPDH is used as loading control. n = 4 per group. Data represent the mean ± SEM of three replicates. ∗ P < 0.05, ∗ ∗ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-03389-1'>33436548</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2018_1029_fig6_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;The texts overlap in figure 6g. So I want to replace it with a new in the attachment. Kcnq1ot1/miR-214-3p pathway regulates inflammation in fibroblasts a , b qRT-PCR was conducted to detect expression of NLRP3 and IL-1β. c , d The expression levels of NLRP3 and IL-1β were determined by immunofluorescence. e – g Western blot was performed to determine the expression of NLRP3, IL-1β, and GSDMD-N. * P < 0.05 compared with the si-NC + AMO-NC group, # P < 0.05 compared with the HG + si-Kcnq1ot1 + AMO-NC group, & P < 0.05 compared with the HG + si-Kcnq1ot1 + AMO-214-3p group. n = 3 in each group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-018-1029-4'>30250027</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig8_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;The mechanism by which the lncRNA NLRP3/miR-138-5p/NLRP3 ceRNET functions in the inflammatory response. The lungs of rats were injected with PBS in the control group and LPS-treated rats were further treated with si-r-lncRNA NLRP3, Lv-lncRNA NLRP3, agomiR-138-5p, antagomiR-138-5p, Lv-lncRNA NLRP3 + agomiR-138-5p, and si-r-lnc NLRP3 + antagomiR-138-5p. A Morphometric changes in the appearance of the lungs that had been fixed in 4% paraformaldehyde for 24 h at 25 °C in each group. B , C The protein expression levels of NLRP3 and caspase-1 in rat lung tissues. qRT-PCR assays were used to analyse mRNA expression of D lncRNA NLRP3, E NLRP3, F IL-18, G Caspase-1, H IL-1β, and I miR-138-5p in the lung tissues of rats. ELISA analysis of the IL-1β ( J ) and IL-18 ( K ) levels in the culture supernatant. L Graphical summary of the role of the lncRNA NLRP3/miR-138-5p/NLRP3 ceRNET in acute lung injury. β-Actin was used as the reference. The data are presented as mean ± SE ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2023_6237_fig6_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;ChemR23 activation ameliorated pyroptosis after chronic hypoxic stimulation in primary rat hippocampal neurons. A Changes in LDH release in the cell culture supernatant with time under hypoxic conditions. n = 4 per group. B Effects of different concentrations of RvE1 and C-9 on the release of LDH in primary neurons under hypoxic stimulation. n = 4 per group. C Cell viability of each group under different treatment conditions. n = 3 per group. D Fluorescence images of primary neurons stained with calcein AM (live cells, green fluorescence) and PI (dead cells, red fluorescence) after different treatments. Scale bar: 50 μm. n = 3 per group. E Double immunostaining of NLRP3 with MAP2 in primary neurons of different groups. Scale bar: 100 μm. n = 3 for each group. F Double immunostaining of GSDMD with MAP2 in primary neurons of different groups. Scale bar: 100 μm. n = 3 for each group. G The statistics of the mean fluorescence intensity of NLRP3 in each group. n = 3 for each group. H The statistics of the mean fluorescence intensity of GSDMD in each group. n = 3 for each group. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Con; #P < 0.05, ##P < 0.01 vs. Hypoxia. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-023-06237-6'>37932279</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2023_6237_fig7_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Effects of ChemR23 activation on pyroptosis in SH-SY5Y cells after hypoglycaemic and hypoxic stimulation. A Changes in cell viability with time under hypoglycaemic and hypoxic conditions. n = 4 per group. B , C Effects of different concentrations of RvE1 and C-9 on cell viability. n = 4 per group. D , E The proportion of Annexin V/PI double positive cells analysed by flow cytometry. n = 3 per group. F LDH levels in the cell culture supernatant. n = 3 per group. G – K Western blot analysis of NLRP3, GSDMD-N, Caspase-1 p20 and ASC. n = 3 per group. L , M Concentrations of IL-1β and IL-18 in the cell culture supernatant were measured by ELISA. n = 4 per group. (N) Representative immunofluorescence staining of NLRP3 (green) with the nuclei counterstained by DAPI. Scale bar: 50 μm. n = 3 per group. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001 vs. Con; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. HH; &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. HH+RvE1; †P < 0.05, ††P < 0.01, †††P < 0.001 vs. HH + C-9. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-023-06237-6'>37932279</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12967_2023_4813_fig7_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;The neuroprotective effect of ChemR23 against OGD-induced neuronal pyroptosis was NLRP3 dependent. A Representative immunofluorescent images of NLRP3 in cultured neurons. Scale bar = 20 μm. B , C Immunoblots and quantitative analysis of NLRP3, ASC, caspase-1 p20, GSDMD-N in treated neurons after OGD. D Representative scanning electron microscopy pictures of neurons. Scale bar = 10 μm. E LDH release was assessed and quantified in neurons subjected to OGD for 4 h. F , G ELISA analysis for IL-1β and IL-18 levels in supernatants of neuron subjected to OGD for 4 h. At least three independent experiments were repeated. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04813-0'>38178174</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2023_6237_fig4_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Effects of ChemR23 activation with RvE1 or C-9 on neuronal pyroptosis in CCH rats. A Representative transmission electron micrographs of hippocampal neurons in different treatment groups. Black arrowhead: membrane pores. Scale bar: 2 μm. n = 3 per group. B , C Representative double immunofluorescence staining of GSDMD/NeuN and NLRP3/NeuN in the rat hippocampus. Scale bar: 20 μm. n = 3 per group. D , E Quantitative statistics of GSDMD/NeuN or NLRP3/NeuN double-positive cells in rat hippocampus. n = 3 per group. F , G ELISA results of IL-1 and IL-18 analysis in the rat hippocampus. n = 4 per group. H , I Representative immunoblotting bands and the expression levels of NLRP3, GSDMD-N, Caspase-1 p20 and ASC in the rat hippocampus. n = 4 per group. Data are presented as the mean ± SEM. **P < 0.01, ***P < 0.001 vs. sham; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. CCH. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-023-06237-6'>37932279</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12967_2023_4813_fig5_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;RvE1 and C-9 ameliorated NLRP3 inflammasome-mediated pyroptosis in MCAO mice. A , B Immunoblots and quantitative analysis of NLRP3, ASC, caspase-1 p20, GSDMD-N (n = 3/per group). C – E Double immunofluorescent staining of NLRP3 or GSDMD with NeuN in ischemic penumbra region and quantitative analysis at Day 1 after MCAO (n = 3/per group). Scale bar = 20 μm. F , G The ELISA assays for IL-1β and IL-18 levels in ipsilateral brain tissues subjected to ischemia at Day 1 (n = 4/per group). H Representative microphotographs and quantification of neuronal death based on NeuN and TUNEL assay in the ischemic ipsilateral brain regions at Day 1 after MCAO (n = 3/per group). Scale bar = 2 μm. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04813-0'>38178174</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12967_2023_4813_fig2_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;ChemR23 deletion amplified NLRP3 inflammasome activation in cerebral ischemia injury. A , B Thermograms and volcano plots showing the comparison between ChemR23 KO group and WT group at Day 1 after MCAO (n = 4/per group). C The KEGG pathway enrichment analysis (n = 4/per group). D qPCR analysis of the infammasome sensor genes NLRP1, NLRP3, NLRC4, and AIM2 ipsilateral hemispheres of WT mice and KO mice subjected to MCAO at Day 1 (n = 3/per group). E – H Western blotting and quantitative analysis of NLRP3, ASC, caspase-1 p20 expression in ischemic penumbra tissue at 12 h, Day 1 and Day 3 after MCAO (n = 3/per group). I , J Representative immunofluorescence staining images of NLRP3 were co-stained with NeuN in peri-infarct areas and their quantification at Day 1 after MCAO (n = 3/per group). Scale bar = 20 μm. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-023-04813-0'>38178174</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12931_2024_2986_fig3_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Added endoplasmic reticulum stress (ERS) 4-PBA can restrain pyroptosis caused by hypoxia. A , Western blot indicated adding 4-PBA can inhibit NLRP3, GSDMD and Caspase 1 expression during hypoxia ( n = 5–6). B , The positive cells were demonstrated by PI staining ( n = 3). C , LDH release assay detected the content of lactate dehydrogenase ( n = 6). D , Immunofluorescence showed inhibitor of ERS reduce IL-1β expression in the hypoxia ( n = 6). E , Fluorescent probe DCFH-DA detected ROS release of cells treated with 4-PBA ( n = 6). Scale bars, 50 μm. All values are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12931-024-02986-w'>39354535</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-fimmu-10-01003-g007.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Histone induce BMECs pyroptosis is dependent on activations of caspase 1, caspase 3 and NLRP3. BMECs were incubated with stimulated histone for 16 h, and the activities of these proteins were determined by western blotting. Date are presented as mean ± SEM ( n = 5). P -values of < 0.05 were considered significant (** P < 0.01, *** P < 0.001, and “ns” means not significant).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.01003/full'>31156617</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2023_6237_fig8_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;ChemR23 activation inhibited neuronal pyroptosis via the PI3K/AKT/Nrf2 signalling pathway in SH-SY5Y cells. A Scanning electron microscopy analysis showing the cell morphology in different treatment groups. n = 3 per group. B Representative immunofluorescence staining of NLRP3 (green) with the nuclei counterstained by DAPI. Scale bar: 50 μm. n = 3 per group. C LDH levels in the cell culture supernatant. n = 3 per group. D , E ELISA of IL-1β and IL-18 levels. n = 3 per group. F – J The levels of NLRP3, GSDMD-N, Caspase-1 p20 and ASC were evaluated by western blot. n = 3 per group. K – N The expression levels of p-PI3K/PI3K, p-AKT/AKT and Nrf2 were evaluated by western blot. n = 3 per group. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Con; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. HH; &P < 0.05, &&P < 0.01, &&&P < 0.001 vs. HH + OE; N.S. means not significant vs. HH + OE. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-023-06237-6'>37932279</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2018_1029_fig2_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Kcnq1ot1 is involved in the regulation of pyroptosis in vivo a Immunohistochemistry analysis was performed to detect the expression NLRP3, caspase-1, IL-1β, and GSDMD-N. Scale bar, 20 μm. b – d qRT-PCR was conducted to analyze the mRNA expression of NLRP3, caspase-1 and IL-1β. e Western blot was conducted to determine the protein expression of NLRP3, caspase-1 and IL-1β. * P < 0.05 compared with the control group, # P < 0.05 compared with the DM + Scr-shRNA group. n = 5 in each group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-018-1029-4'>30250027</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12974_2022_2494_fig3_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Activation of Rev-erbα inhibited microglial activation induced by MPP + and αsyn PFF. The representative western blot bands ( A ) and the statistical graph ( B – D ) of p-NF-κB p65, NLRP3, ASC, cleaved caspase-1, IL-1β, IL-18, IL-6, TNF-a, iNOS, Arg-1 and CD163 protein expressions. BV2 cells were pretreated with SR9009 (2 μM, 5 μM, 10 μM) for 1 h, then incubated with MPP + for 24 h. The representative western blot bands ( E ) and the statistical graph ( F–H ) of p-NF-κB p65, NLRP3, ASC, cleaved caspase-1, IL-1β, CD68 and αsyn protein expressions. BV2 cells were pretreated with SR9009 (2 μM, 5 μM, 10 μM) for 1 h, then incubated with αsyn pre-formed-fibril for 6 h. The p-NF-κB p65 level was normalized to the total of NF-κB p65, and the rest protein levels were normalized to β-actin. Data were presented as mean ± SEM ( n = 3). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** or p < 0.0001 by One-way ANOVA test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-022-02494-y'>35668454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665_fcell-13-1577669-g006.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;CPT1A protects against SAP in mice by inhibiting the NLRP3/GSDMD-mediated pyroptosis signalling pathway. (A–D) Immunofluorescence staining of NLRP3 and GSDMD in the pancreas of mice received different treatments. Scale bar: 100 μm. (E–G) Protein levels of cleaved Caspase-1 and GSDMD-NT in the pancreas of mice received different treatments. β-actin served as the loading control. All data are presented as means ± SEM, n = 3–5. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Control group. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. STC group. & p < 0.05, &&& p < 0.001 vs. LC (100 mg/kg) treatment group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12289652/'>40718711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12974_2022_2494_fig4_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Rev-erbα regulates microglial activation by NF-κB inflammasome pathway. The representative western blot bands ( A ) and the statistical graph ( B – D ) of p-NF-κB p65, NLRP3, ASC, cleaved caspase-1, IL-1β, IL-18, IL-6, TNF-a, iNOS, Arg-1 and CD163 protein expressions. BV2 cells were pretreated with JSH-23 (10 μM) or SR8278 (10 μM) for 1 h, then incubated with MPP + for 24 h. The p-NF-κB p65 level was normalized to the total of NF-κB p65, and the rest protein levels were normalized to β-actin. Data were presented as mean ± SEM (n = 3). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** or p < 0.0001 by One-way ANOVA test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-022-02494-y'>35668454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12931_2024_2986_fig2_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Over-expression of circSSR1 inhibited vascular smooth muscle cell pyroptosis. A , Over-expression of circSSR1 reduce reactive oxygen species probes (DCFH-DA, green) staining in various groups ( n = 6). B , Endoplasmic reticulum stress (ERS) related protein ATF6, IRE1 and GRP78 expression. C , LDH cytotoxicity assay kit (LDH) was used to detect LDH release ( n = 6). D , Western blot to demonstrate pyroptosis related protein NLRP3, GSDMD and Caspase-1 expression ( n = 5). E , Fluorescence staining of IL-1β in PASMCs under hypoxia ( n = 6). F , Over-expression of circSSR1 plasmid decreased PI positive cells ( n = 5). Scale bars, 50 μm. All values are presented as the mean ± SEM. *p<0.05, **p<0.01, ***p<0.001. NOR, normoxia and HYP, hypoxia. NC: negative control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12931-024-02986-w'>39354535</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12974_2022_2494_fig5_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Rev-erbα regulates microglial activation by NLRP3 inflammasome pathway. The representative western blot bands ( A ) and the statistical graph ( B – D ) of p-NF-κB p65, NLRP3, ASC, cleaved caspase-1, IL-1β, IL-18, IL-6, TNF-a, iNOS, Arg-1 and CD163 protein expressions. BV2 cells were pretreated with MCC950 (10 μM) or SR8278 (10 μM) for 1 h, then incubated with MPP + for 24 h. The p-NF-κB p65 level was normalized to the total of NF-κB p65, and the rest protein levels were normalized to β-actin. Data were presented as mean ± SEM ( n = 3). (* p < 0.05, ** p < 0.01, *** p < 0.001, **** or p < 0.0001 by One-way ANOVA test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-022-02494-y'>35668454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_3389_fig2_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;MCC950 and RVS protect against CME-induced cardiac dysfunction and injury. A Representative M-mode echocardiograms for each group at 3 days after CME intervention. Mice were treated with RVS and the selective NLRP3 inflammasome inhibitor MCC950 three days prior and after CME intervention at 40 and 20 mg/kg/d respectively. B Left ventricular ejection fraction (LVEF) and fractional shortening (FS) are measured using Doppler echocardiography. n = 8 to 10 per group. Data represent the mean ± SEM. C Representative images of Heidenhain’s iron hematoxylin staining for each group to visualize microinfarct areas, the microinfarct areas are stained into dark gray. D The quantitative analysis of microinfarct areas. n = 8 to 10 per group. The quantification is representative of the percentage of microinfarct area in 5 fields ± SEM by random. E Serum lactate dehydrogenase (LDH) levels are measured in each group. n = 8 to 10 per group. Data represent the mean ± SEM. F Representative images of HE staining to visualize the local micro-infracted lesions for each group. G Representative images of Masson trichrome staining of the ventricular sections of each group. H The quantitative analysis of collagen contents. n = 8 to 10 per group. The quantification is representative of the percentage of collagen contents in 5 fields ± SEM by random. Scale bars = 50 µm. Black arrows indicate the microspheres. ∗ P < 0.05, ∗ ∗ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-03389-1'>33436548</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12974_2022_2494_fig11_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;SR9009 suppresses NLRP3 inflammasome activation in the SN of MPTP-induced mice. The representative western blot bands ( A ) and the statistical graph ( B ) of p-NF-κB p65 and NF-κB p65 in the SN. The representative western blot bands ( E ) and the statistical graph ( C – D , F ) of NLRP3, ASC and cleaved-caspase-1 in the SN. The real-time PCR results of IL-1β ( G ) and IL-18 ( H ) in the SN. I Representative double-immunofluorescent staining of IBA1 (red) and NLRP3 (green) in the SN and the statistical graph ( J ) of IBA1 + NLRP3 + /IBA1 + cells. Scale bar, 50 μm. n = 3–4 for each group. The p-NF-κB p65 level was normalized to the total of NF-κB p65, and the rest protein levels were normalized to β-actin. Data were presented as mean ± SEM. (* p < 0.05, ** p < 0.01, or *** p < 0.001 by One-way ANOVA test) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-022-02494-y'>35668454</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_3389_fig4_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;RVS and ROS scavenger inhibit the activation of the NLRP3 inflammasome and pyroptosis in H9c2 cells. A The H9c2 cells are stimulated with 40 ng/ml TNF-α combined with hypoxia for 12 h to active the NLRP3 inflammasome. The caspase-1 selective inhibitor VX-765 and ROS scavenger N-acetylcysteine (NAC) are added 2 h before the stimulation at 20 μM and 5 mM respectively. The pyroptotic cells are determined by Hoechst 33342/PI staining, wherein the nuclei are stained to blue by Hoechst 33342, and the pyroptotic cells are stained to red by PI. Scale bars = 50 μm. B The quantitative analysis of PI positive cells. n = 6 per group. The quantification is representative of the percentage of pyroptotic cells in 5 fields ± SEM by random. C LDH release is measured by a cytotoxicity detection LDH kit. n = 6 per group. D Cell viability is measured by a CCK-8 kit, and the optical densities (ODs) are compared among the groups. n = 6 per group. E Representative immunoblots for NLRP3, caspase-1, cleaved-caspase-1, GSDMD, GSDMD-N in H9c2 cells. F The densitometric analysis of relative protein expressions. Vinculin is used as a loading control. n = 4 per group. G Cells with active caspase-1 in each group are stained with FLICA (FAM-YVAD-FMK) probe and detected using flow cytometer. H The percentages of caspase-1 positive cells are compared among the groups. n = 6 per group. Data represent the mean ± SEM. All experiments were repeated three times. ∗ P < 0.05, ∗ ∗ P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-03389-1'>33436548</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig1_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Dysregulated transcription of lncRNA NLRP3 and NLRP3 in LPS-treated NR8383 AM cells as determined by RNA-seq and bioinformatics analysis. A The heat map lists the top 20 differentially expressed lncRNAs and mRNAs in NR8383 AM after treatment with PBS, LPS for 2 h, and LPS for 9 h. A , B RNA-seq analysis shows the quantified gene expression of lncRNA NLRP3 and NLRP3 in AM cells in the negative control, LPS 2 h, and LPS 9 h groups. C , D Agarose gel electrophoresis analysis shows the quantified expression of lncRNA NLRP3 and NLRP3 in NR8383 cells. β-Actin served as the control. E The conservation of lncRNA NLRP3 was predicted and analysed by the UCSC Genome Browser. F The lncRNA NLRP3 potential protein-coding and binding sites were analysed with RNA 2.0 tools. G The results show that lncRNA NLRP3 has no protein-coding capability. H Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis were used to analyze differentially expressed genes. I The relationship between lncRNA NLRP3 and NLRP3, and the correlation coefficient is listed. * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12964_2019_406_fig2_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;High-salt enhances NLRP3 inflammasome activation in ECs. a Quantification of mRNA levels of IL-1β in TA and AA of ApoE −/− mice fed with a normal or high-salt diet for 4 weeks. b Quantification of protein levels of IL-1β in the serum of ApoE −/− mice fed with a normal or high-salt diet for 4 weeks. c, d Quantification of mRNA levels and protein levels of NLRP3 by HUVECs in hypertonic medium (290, 310, 330 and 350 mosmol/kg) with isomolar 270 mosmol/kg as the control, evaluated by RT-qPCR and Western blotting with β-actin as the internal control. e Immunofluorescent staining of NLRP3 in HUVECs with iso- and hyper-osmotic media. f, g mRNA and protein expression of IL-1β in HUVECs exposed to iso- and hyper-osmotic media, evaluated by RT-qPCR and ELISA. All data were presented as mean ± SEM, N ≥ 3. * p < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12964-019-0406-7'>31429763</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig2_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Effects of LPS on the lncRNA NLRP3, miR-138-5p, NLRP3, Caspase-1, IL-1β, and IL-18 expression levels in early ALI. A qRT-PCR assay was used to analyse the mRNA expression of A LncRNA NLRP3, B miR-138-5p, C NLRP3, D Caspase-1, E IL-1β, and F IL-18 in LPS-induced ALI. β-Actin was used as the reference gene. G , H ELISA analysis of the IL-1β and IL-18 levels in the culture supernatant. Cell apoptosis was determined by Hoechst 33342 and PI dual staining assays ( I ) and counted ( J ). The expression of NLRP3 and caspase-1 in the NR8383 AM cells from the four groups was analysed by western blotting ( K ). L Expression trends of lncRNA NLRP3, NLRP3, caspase-1, IL-1β, IL-18, and miR-138-5p in the negative control group and groups treated with LPS for 6, 12, and 24 h. The data are presented as mean ± SE ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12964_2019_406_fig5_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;High-salt activates NRLP3 inflammasome in ECs via NFAT5. a Immunoblot of NFAT5, caspase-1 p20, pro-IL-1β, and IL-1β p17, and quantification of caspase-1 activity and mature IL-1β in ECs treated with Adenovirus-null (Ad-null, 5 MOI) and Adenovirus-NFAT5 (Ad-NFAT5, 2 MOI or 5 MOI). See Additional file : Figure S4 for caspase-1 activity. b Immunoblot images of NFAT5, caspase-1 p20, pro-IL-1β, and IL-1β p17, and quantification of active caspase-1 and mature IL-1β in ECs treated with high-salt and transfected with Ctr siRNA or NFAT5 siRNA. c Immunoblot images of NLRP3, caspase-1 p20, pro-IL-1β, and IL-1β p17, and quantification of active caspase-1 and mature IL-1β in ECs treated with high-salt and transfected with Ctr siRNA or NLRP3 siRNA. d Immunoblot images of NLRP3, caspase-1 p20, pro-IL-1β, and IL-1β p17, and quantification of active caspase-1 and mature IL-1β in ECs treated with high-salt, treated with NAC. All data were presented as mean ± SEM, N ≥ 3. * p < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12964-019-0406-7'>31429763</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig3_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;LncRNA NLRP3 regulates the inflammatory response during ALI through NLRP3 inflammasomes. A qRT-PCR assay was used to analyse the mRNA expression of A lncRNA NLRP3, B NLRP3, C Caspase-1, D IL-18, E IL-1β, and F miR-138-5p in LPS -induced ALI. β-Actin was used as the reference gene. G , H ELISA was used to analyse the IL-1β and IL-18 levels in the culture supernatants. I , J Cell apoptosis was determined by Hoechst 33342 and PI dual staining assays ( I ) and counted ( J ). K Western blotting was used to analyse the protein expression of NLRP3 and caspase-1 after lncRNA NLRP3 overexpression in the cytoplasm. The data are presented as mean ± SE ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-12964_2019_406_fig7_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Schematic summarizes the mechanism that NLRP3 inflammasome activation in endothelium mediates hypertonic stress-induced atherosclerosis via NFAT5. Schematic illustration of the process. Stage i: Hypertonicity → NFAT5-dependent NLRP3 gene transcription → NLRP3 inflammasome activation. Stage ii: NLRP3 inflammasome activation → NFAT5-transcription-mediated pro-IL-1β → IL-1β secretion. Stage iii: IL-1β secretion → adhesive molecules → monocytes adhesion and infiltration. Stage iv: The activation of endothelial innate immunity promotes macrophage-driven foam cells and phenotype conversion of smooth muscle cells, contributing to the formation of atherosclerosis <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12964-019-0406-7'>31429763</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig4_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;LncRNA NLRP3 functions as a sponge for miR-138-5p. A LncLocator was used to investigate the distribution of lncRNA NLRP3. B LncRNA NLRP3 was mainly located in the cytoplasm. C Venn diagram of miRDB predicting miR-138-5p and miR-370-3p sponged by lncRNA NLRP3 and NLRP3. D , E The predicted miR-138-5p-binding sites in the lncRNA NLRP3 3′-UTR. F miR-138-5p mimics notably reduced the luciferase activity of the lncRNA NLRP3-Wt group. G silncRNA NLRP3 significantly increased miR-138-5p expression; however, overexpression of lncRNA NLRP3 reduced miR-138-5p expression in LPS-treated NR8383 AM cells. H RIP assays revealed that Ago2-containing beads enriched the expression of miR-138-5p and NLRP3. I The miR-138-5p inhibitor and miR-138-5p mimics had no effects on lncRNA NLRP3 expression in LPS-treated NR8383 AM cells. The data are presented as mean ± SE ( n = 6). J LncRNA NLRP3 expression was negatively correlated with miR-138-5p expression in LPS-treated NR8383 AM cells. * P < 0.05; ** P < 0.01; *** p < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-fimmu-10-01003-g008.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Immunocytochemistry analysis of histone induced activations of caspase 1, caspase 3, and NLRP3 (400×). BMECs were cultured briefly on cover glasses (pre-treated with poly-L –lysine, 0.1 mg/mL, Sigma-Aldrich) and incubated with histone (50 and 100 μg/mL) for 16 h. The samples were visualized with DAB, counterstained with hematoxylin and observed by inverted microscope. Three independent experiments were carried out by light microscope analyses.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2019.01003/full'>31156617</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig5_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;miR-138-5p regulates the inflammatory response by targeting NLRP3. A The predicted miR-138-5p-binding sites in the NLRP3 mRNA 3′-UTR. B A firefly luciferase reporter containing either wild-type or mutant NLRP3 was cotransfected into NR8383 AM cells with miR-138-5p mimics NC or miR-138-5p mimics. qRT-PCR assays were used to analyse the mRNA expression of C miR-138-5p, D NLRP3, E Caspase-1, F IL-18, and G IL-1β in the NR8383 AM cells ( n = 6). β-Actin was used as a reference gene. H , I ELISA was used to analyse the IL-1β and IL-18 levels in the culture supernatants. J Western blotting assay of the protein expression levels of NLRP3 and Caspase-1. K , L Cell apoptosis was determined by Hoechst 33342 and PI dual staining assays ( K ) and counted ( L ). The data are presented as mean ± SE ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2018_1029_fig3_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Kcnq1ot1 and pytoptosis are activated in HG-treated cardiac fibroblasts qRT-PCR was performed to measure the Kcnq1ot1 expression level in the serums of non-diabetic and diabetic patients a . * P < 0.05 compared with the non-diabetic group. n = 6 in each group. Cardiac fibroblasts of neonatal C57BL/6 mice were incubated with 5.5 mmol/L glucose (Control) or 30 mmol/L (high glucose, HG) for 24 h. The expression levels of Kcnq1ot1 were detected by qRT-PCR b . The expression levels of NLRP3, caspase-1 and IL-1β were determined by qRT-PCR c , immunofluorescence d – g and western blot h , i . * P < 0.05 compared with the control group. n = 3 in each group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-018-1029-4'>30250027</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig6_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;LncRNA NLRP3 regulates the inflammatory response through the lncRNA NLRP3/miR-138-5p/NLRP3 ceRNET in vitro. miR-138-5p suppression reversed the effects of silncRNA NLRP3 on the mRNA expression of A lncRNA NLRP3, B NLRP3, C Caspase-1, D IL-1β, E IL-18, and F miR-138-5p in NR8383 alveolar macrophage (AMs) cells. β-Actin was used as the reference gene. G , H ELISA analysis of the IL-1β and IL-18 levels in the culture supernatant. I Western blotting assay of the protein expression levels of NLRP3 and caspase-1. J , K Cell apoptosis was determined by Hoechst 33342 and PI dual staining assays ( J ) and counted ( K ). The data are presented as mean ± SE ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665_fcell-13-1577669-g004.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;LC protects against STC-induced acinar cell pyroptosis via CPT1A. (A,B) Representative images and quantification of Hoechst 33342 (blue) and PI (red) staining of primary acinar cells received different treatments. Scale bar: 50 μm. (C,D) JC-1 fluorescent staining of primary acinar cells in different treatment groups. Scale bar: 50 μm. (E) The release of ATP levels in the supernatant of cultured primary acinar cells received different treatments. (F) Quantification of mtROS in primary acinar cells in different treatment groups. (G) The ratio of 8-OHdG to total mtDNA content in primary acinar cells received different treatments. (H) Protein levels of CPT1A and β-actin in the primary acinar cells received different treatments. (I,J) Protein levels of NLRP3, cleaved Caspase-1 and GSDMD-NT in the primary acinar cell received different treatments. β-actin served as the loading control (n = 3). Data are presented as means ± SEM, n = 3–5. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Control group. # p < 0.05, ## p < 0.01, ### p < 0.001, vs. STC group. & p < 0.05, && p < 0.01, &&& p < 0.001 vs. STC+LC treatment group. STC: sodium taurocholate; LC: L-carnitine; Eto: Etomoxir.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12289652/'>40718711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-41419_2021_4180_fig7_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;LncRNA NLRP3/miR-138-5p/NLRP3 functions via the ceRNET during the NLRP3-triggered inflammatory response in vivo. Rat lungs were injected with PBS in the control group and LPS-treated rats were further treated with si-r-lncRNA NLRP3, Lv-lncRNA NLRP3, agomiR-138-5p, antagomiR-138-5p, Lv-lncRNA NLRP3 + agomiR-138-5p, and si-r-lncRNA NLRP3 + antagomiR-138-5p. A Lung tissue samples were collected 6 h after establishing LPS-induced ALI to analyse the histopathological changes (×200, ×400). The black arrow indicates neutrophil infiltration, pulmonary oedema, alveolar wall thickening, and alveolar haemorrhage. B The lung injury score was determined via H&E staining, a representative histological analysis ( n = 6 animals per group). C ELISA was used to measure the BALF albumin content. D Detection of the lung W/D ratio in rats. E MPO activity in the lung tissues of rats. F , G Immunohistochemical detection of the NLRP3 contents in rat lung tissues (×200, ×400). H The inflammatory response in NR8383 AM cells was suppressed by si-r-lncRNA NLRP3 and miR-138-5p mimics alone or in combination, as shown by the decreased number of cells colabeled with CD68 (green) and NLRP3 (red). LncRNA NLRP3 overexpression, miR-138-5p inhibition, and NLRP3 augmented the inflammatory response in LPS-induced ALI with more NLRP3 and CD68 anchored in the plasma membrane of the AM cells. The data are presented as mean ± SE ( n = 6). * P < 0.05; ** P < 0.01; *** P < 0.001; NS, no statistically significant difference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-021-04180-y'>34599154</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1665-13195_2025_1714_fig5_html.pngAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg;Effect of PBM on NLRP3 inflammasome in mice with AD. A Exemplary WB and quantification of NLRP3 in mouse brain tissue. B Representative immunofluorescence images of NLRP3 (red), anti-GFAP (orange) and anti-IBA1 (green) antibodies in the mouse hippocampus and cortex (scale bars 100 μm and 20 μm, respectively). C MCC scores between astrocyte (Ch1) and NLRP3 (Ch2) (tM1, above the auto-threshold of Ch2). D MCC scores between microglia (Ch1) and NLRP3 (Ch2) (tM1, above the auto-threshold of Ch2). Experimental data are presented as the mean ± standard deviation. Compared with the CON group, * p < 0.05, ** p < 0.01, *** p < 0.001; Compared with the AD group, ### p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13195-025-01714-w'>40188044</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nox1-antibody-pa1666-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1666-nox1-primary-antibodies-wb-testing-1.jpgAnti-NADPH oxidase 1 NOX1 Antibody Picoband&reg; Western blot analysis of NOX1 using anti-NOX1 antibody (PA1666). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX1 antigen affinity purified polyclonal antibody (Catalog # PA1666) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX1 at approximately 65 kDa. The expected band size for NOX1 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1666-ijbsv15p1240g007.jpgAnti-NADPH oxidase 1 NOX1 Antibody Picoband&reg;Effect of GDM on the ROS level in offspring. Heart tissues were isolated from male offspring. ( A ) ROS levels in the left ventricle (LV) tissues isolated from both control (□) and STZ-treated (■) groups were measured using in vitro ROS/RNS assay kit. ( B ) NOX1, 2, and 4 protein abundances in the LV tissues isolated from both control (□) and STZ-treated (■) groups were determined by Western blot analysis. Their protein densities were normalized to internal control (GAPDH). ( C ) After NAC pretreatment, ROS levels in the LV tissues isolated from both control (□) and STZ-treated (■) groups were measured using in vitro ROS/RNS assay kit. Data are means ± SEM (n=4 animals/group). *P < 0.05 vs. control, as determined by Student's t-test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6567811/&orig_host=www.ncbi.nlm.nih.gov'>31223283</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1666-nox1-primary-antibodies-ihc-testing-2.jpgAnti-NADPH oxidase 1 NOX1 Antibody Picoband&reg; IHC analysis of NOX1 using anti-NOX1 antibody (PA1666). <br> NOX1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOX1 Antibody (PA1666) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nox2-gp91phox-antibody-pa1667-boster.html2026-04-01T05:01:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1667-fphar-13-812594-g002.jpgAnti-NOX2/gp91phox/CYBB Antibody Picoband&reg;ASIV reduced ADR-induced oxidative stress. (A) Representative images of DHE staining and quantification of ROS production. DHE positive (red), nuclear stained with DAPI (blue). Serum GPx (B) , SOD (C) , and MDA (D) levels among the different groups ( n = 3). (E) Protein expression of oxidative stress-associated proteins, including NOX2, NOX4 in kidney tissue. (F,G) The expression of NOX2 and NOX4. Data were expressed as mean ± SD, n = 6. * p < 0.05, ** p < 0.01 vs. CON group. # p < 0.05, ## p < 0.01 vs. ADR group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8971812/&orig_host=www.ncbi.nlm.nih.gov'>35370757</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1667-ajtr0011-2925-f2.jpgAnti-NOX2/gp91phox/CYBB Antibody Picoband&reg;Brain RAS, oxidative stress and sympathetic activity were up-regulated in DM rats. A. AGT and AT1 receptors in SFO (a1), SON (a2) and PVN (a3) measured by immunohistochemistry. B. AGT and AT1 receptors in SFO (b1), SON (b2) and PVN (b3) measured by Western blot. C. Protein levels of NOX2 and NOX4 in SFO (c1), SON (c2) and PVN (c3) measured by Western-blot. D. Representative photographs of TH+c-fos positive cells in RVLM measured by immunohistochemistry. E. Protein levels of TH in RVLM measured by Western-blot. F. Protein levels of TH in SFO, SON, PVN measured by Western-blot. Data are expressed as the mean ± SD (n=15 in each group). * P <0.05 versus Non-DM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1667-ajtr0011-2925-f6.jpgAnti-NOX2/gp91phox/CYBB Antibody Picoband&reg;Expression of RAS components, NOXs and TH in brain nuclei and kidney measured by western-blot. A. Protein levels of AGT (a1) and AT1 receptors (a2) in brain nuclei measured by Western-blot. B. Protein levels of NOX2 (b1) and NOX4 (b2) in brain nuclei measured by Western-blot. C. Protein expression of TH in SFO, SON PVN (c1) and RVLM (c2) measured by western-blot. D. Protein levels of AGT, AT1, MCP-1 (d1) and Noxs (d2) in renal cortex homogenates measured by Western-blot. Data are expressed as the mean ± SD (n=6 in each group). * P <0.05 versus IG 0 mg/kg/d Los.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1667-cybb-primary-antibodies-wb-testing-1_1.jpgAnti-NOX2/gp91phox/CYBB Antibody Picoband&reg; Western blot analysis of CYBB using anti-CYBB antibody (PA1667). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: rat RH35 whole cell lysates,<br> Lane 5: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYBB antigen affinity purified polyclonal antibody (Catalog # PA1667) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYBB at approximately 65 kDa. The expected band size for CYBB is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1667-cybb-primary-antibodies-ihc-testing-2_1.jpgAnti-NOX2/gp91phox/CYBB Antibody Picoband&reg; IHC analysis of CYBB using anti-CYBB antibody (PA1667). <br> CYBB was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYBB Antibody (PA1667) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1667-nox2gp91phoxcybb-primary-antibodies-wb-testing-3.pngAnti-NOX2/gp91phox/CYBB Antibody Picoband&reg; Western blot analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody(PA1667). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2/gp91phox/CYBB antibody(PA1667) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Cell Signaling Technology's 7074s murine anti-rabbit conformation specific antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX2/gp91phox/CYBB at approximately 65 kDa. The expected band size for NOX2/gp91phox/CYBB is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-oncostatin-m-antibody-pa1668-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1668-1-WB-anti-osm-oncostatin-m-antibody.jpgAnti-Oncostatin M/OSM Antibody Picoband&reg;Anti-Oncostatin M antibody&#44; PA1668&#44; Western blotting<br>All lanes: Anti Oncostatin M (PA1668) at 0.5ug/ml<br> Lane 1: A549 Whole Cell Lysate at 40ug<br> Lane 2: A549 Whole Cell Lysate at 40ug<br> Lane 3: HELA Whole Cell Lysate at 40ug<br> Predicted bind size: 28KD<br> Observed bind size: 28KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1668-2-IHC-anti-osm-oncostatin-m-antibody.jpgAnti-Oncostatin M/OSM Antibody Picoband&reg;Anti-Oncostatin M antibody&#44; PA1668&#44; IHC(P)<br>IHC(P):Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nf-kb-p65-antibody-pa1669-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-12872_2022_3003_fig1_html.pngAnti-NF-kB p65/RELA Antibody Picoband&reg;Changes in viability and apoptosis rate of HUVECs during ox-LDL-induced damage. A Cell viability after treatment with different concentrations of ox-LDL (25, 50, 100, or 150 μg/mL) detected using a CCK8 kit. B Cell viability at different time points (12, 24, or 48 h) when HUVECs were cultured with 100 μg/mL ox-LDL. C Cell cytotoxicity after treatment with different concentrations of ox-LDL detected using a LDH kit. D Cell cytotoxicity at different time points when HUVECs were cultured with 100 μg/mL ox-LDL. E Representative flow cytometry profiles of the control and ox-LDL groups. The HUVECs in the control group were untreated and the ox-LDL group was treated with 100 μg/mL ox-LDL for 24 h. F Percentage of apoptotic cells. G Representative western blot images of Cleaved Caspase-3 and Cleaved PARP expression. Original images of western blots are shown in Additional file : Supplementary Fig. 1A–C. The relative protein expression levels of H Cleaved Caspase-3 and I Cleaved PARP. Each experiment was independently repeated three times and the data were expressed as means ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 versus Control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12872-022-03003-y'>36544080</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-12872_2022_3003_fig4_html.pngAnti-NF-kB p65/RELA Antibody Picoband&reg;Inhibition of PI3K-Akt pathway promoted the role of HMGB1. HUVECs were pretreated with 10 μM LY294002 for 1 h. A Representative protein bands showing the expression of Akt, Akt phosphorylation, and NF-κB in HUVECs. GAPDH served as a loading control. Original images of western blots were shown in Additional file : Supplementary Fig. 1I–K. B The protein quantification ratio of P-Akt/Akt. C Relative protein expression levels of NF-κB. Quantitation of D TNF-α, E IL-1β, and F IL-6 in the supernatant of HUVECs was performed by ELISA. G Cell viability in each group detected using a CCK8 assay. H Cytotoxicity detected using a LDH kit. I The percentage of apoptotic cells in each group in J was calculated. J Representative flow cytometry profiles of different treatment groups. ‘+’ was added, ‘−‘ was blank. * p < 0.05, ** p < 0.01, and *** p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12872-022-03003-y'>36544080</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-12974_2021_2198_fig3_html.pngAnti-NF-kB p65/RELA Antibody Picoband&reg;Red nucleus IL-33 facilitates the early development of mononeuropathic pain by activating NF-κB signaling pathway. A Western blotting showed an upregulated NF-κB in the RN at 1 week post-SNI, intrarubral administration of anti-IL-33 antibody restrained the overexpression of NF-κB ( n = 6 per group, F = 12.766, P < 0.001). B Western blotting showed that intrarubral injection of IL-33 stimulated the protein expression of NF-κB in naive rats ( n = 6 per group, F = 7.497, P = 0.003). C Immunohistochemistry demonstrated that NF-κB was increased in the RN of SNI rats ( F = 41.250, P < 0.001) and IL-33-induced hypersensitivity rats ( F = 34.509, P < 0.001) ( n = 4 per group). D Intrarubral injection of NF-κB inhibitor PDTC at 1 week post-injury attenuated SNI-induced mononeuropathic pain compared to DMSO control ( n = 5–6 per group, F = 135.298, P < 0.001). E PDTC pre-injected into the RN, 30 min ahead of IL-33 administration, relieved IL-33-evoked mechanical hypersensitivity compared to DMSO control ( n = 5-6 per group, F = 97.341, P < 0.001). * P < 0.05, ** P < 0.01, and *** P < 0.001. Scale bars = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-021-02198-9'>34225736</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-ijbsv10p0404g003.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Andro suppresses cell proliferation and clonogenicity and induces cell apoptosis in β-TC-6 cells. (A) The cells were treated with various doses of Andro for 48 hours, and the cell viability was quantified by MTT assay. (B) The cells were treated with the IC 50 dose of Andro, and relative cell viability was measured using the MTT assay at the indicated times. (C) The clonogenicity ability of β-TC-6 was significantly suppressed by Andro compared with control groups. Representative photomicrographs are shown. (D) Andro induces cell apoptosis in β-TC-6 cells that treated with the IC 50 dose of Andro for 48 h. Cells were stained with Hoechst 33342 and apoptotic cells were identified by condensation and fragmentation of nuclei using inverted light microscope. All values are presented for the mean ± s.d., n=12; *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC3979993/&orig_host=www.ncbi.nlm.nih.gov'>24719558</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-ijbsv10p0404g004.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;The expression and activation of TLR4/NF-κB signaling is increased during insulinoma development. (A) The classification of insulinoma. The H&E staining defined the stages of insulinoma. (B) The immunohistochemical staining of TLR4, MyD88, p50, p65 and phosphorylated p65 (Ser276) at the stage of normal, hyperplasia, angiogenic islet, insulinoma, and invasive carcinoma in RIP1-Tag2 mice. Results are representative of at least 3 tissue samples in a from more than 3 mice for each stage. Bar, 20 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC3979993/&orig_host=www.ncbi.nlm.nih.gov'>24719558</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-ijbsv10p0404g005.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Andro targets TLR4/NF-κB signaling in insulinoma. Andro can significantly inhibited the protein expression of TLR4, MyD88, phosphorylated IκBα, p65, phosphorylated p65 (Ser536), and phosphorylated p65 (Ser276) of TLR4/NF-κB signaling pathway in tumor tissues of RIP1-Tag2 mice compared with control groups. Immunohistochemical staining showed that Andro also inhibited the expression of TLR4 (B), MyD88 (C) and p65 (D) in tumor tissues of RIP1-Tag2 mice compared with control groups. Bar, 20 μm. *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC3979993/&orig_host=www.ncbi.nlm.nih.gov'>24719558</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-ijbsv10p0404g006.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;TLR4/NF-κB signaling pathway is the dominant functional target of andrographolide . (A) TLR4 deficient could only slightly inhibit the expression of p65 and cannot suppress the expression of MyD88. (B) The inhibition of MyD88 result the reduction of p65 but not TLR4 (C) p65 deficient could inhibit the expression of MyD88 but not TLR4. (D) The inhibition of TLR4, MyD88 and p65 can both suppressed cell proliferation. n=12, * P < 0.05, ** P < 0.01 and *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC3979993/&orig_host=www.ncbi.nlm.nih.gov'>24719558</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-dddt_a_12305505_f0004_c.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;(A) Immunohistochemical images of IL-1β, IL-6, TNF-α, and NF-κB; Mean density: (B) IL-1β; (C) IL-6; (D) TNF-α; (E) NF-κB; (F) Western blot of IL-1β, IL-6, and TNF-α expression; (G) Peripheral blood IL-1β content. Data are presented as mean ± SD. Vs NC, ##P <0.01; Vs PCOS, *P <0.05, **P <0.01; n = 6 per group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/DDDT.S484531'>39247793</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-rela-primary-antibodies-wb-testing-1_1.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg; Western blot analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PA1669). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HT1080 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates,<br> Lane 8: mouse Ana-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65/RELA antigen affinity purified polyclonal antibody (Catalog # PA1669) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65/RELA at approximately 65-70 kDa. The expected band size for NF-kB p65/RELA is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-rela-primary-antibodies-ihc-testing-2.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg; IHC analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PA1669). <br> NF-kB p65/RELA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-kB p65/RELA Antibody (PA1669) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1669-rela-primary-antibodies-if-testing-3_1.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg; IF analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PA1669). <br> NF-kB p65/RELA was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NF-kB p65/RELA Antibody (PA1669) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc10a1-antibody-pa1670-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1670-slc10a1-primary-antibodies-wb-testing-1.jpgAnti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband&reg; Western blot analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human liver tissue lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (Catalog # PA1670) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50 kDa. The expected band size for SLC10A1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1670-slc10a1-primary-antibodies-ihc-testing-4_1.jpgAnti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband&reg;IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670). <br>SLC10A1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC10A1 Antibody (PA1670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1670-slc10a1-primary-antibodies-wb-testing-2.jpgAnti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband&reg; Western blot analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: rat RH35 whole cell lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse liver tissue lysates,<br> Lane 6: mouse HEPA1-6 whole cell lysates,<br> Lane 7: mouse kidney tissue lysates,<br> Lane 8: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (Catalog # PA1670) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50 kDa. The expected band size for SLC10A1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1670-slc10a1-primary-antibodies-ihc-testing-3.jpgAnti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband&reg; IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670). <br> SLC10A1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC10A1 Antibody (PA1670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1670-slc10a1-primary-antibodies-ihc-testing-4.jpgAnti-Sodium/bile acid cotransporter SLC10A1 Antibody Picoband&reg; IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PA1670). <br> SLC10A1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC10A1 Antibody (PA1670) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh1a1-antibody-pa1671-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1671-aldh1a1-primary-antibodies-wb-testing-1.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband&reg; Western blot analysis of ALDH1A1 using anti-ALDH1A1 antibody (PA1671). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: rat kidney tissue lysates,<br> Lane 5: mouse liver tissue lysates,b<br> Lane 6: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A1 antigen affinity purified polyclonal antibody (Catalog # PA1671) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A1 at approximately 55 kDa. The expected band size for ALDH1A1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1671-aldh1a1-primary-antibodies-ihc-testing-2.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband&reg; IHC analysis of ALDH1A1 using anti-ALDH1A1 antibody (PA1671). <br> ALDH1A1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1A1 Antibody (PA1671) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1671-aldh1a1-primary-antibodies-if-testing-3.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband&reg; IF analysis of ALDH1A1 using anti-ALDH1A1 antibody (PA1671). <br> ALDH1A1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALDH1A1 Antibody (PA1671) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1671-aldh1a1-primary-antibodies-fcm-testing-4.jpgAnti-Retinal dehydrogenase 1 ALDH1A1 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-ALDH1A1 antibody (PA1671). <br> Overlay histogram showing HepG2 cells stained with PA1671 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1A1 Antibody (PA1671, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf21-antibody-pa1673-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1673-1-WB-anti-fgf21-antibody.jpgAnti-Fibroblast growth factor 21 FGF21 Antibody Picoband&reg; Western blot analysis of FGF21 using anti-FGF21 antibody (PA1673). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: A549 Whole Cell Lysate&#44;<br> Lane 2: A431 Whole Cell Lysate&#44;<br> Lane 3: HEPA Whole Cell Lysate.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PA1673) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 21KD. The expected band size for FGF21 is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1673-2_1.jpgAnti-Fibroblast growth factor 21 FGF21 Antibody Picoband&reg; Western blot analysis of FGF21 using anti-FGF21 antibody (PA1673). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue Lysate.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PA1673) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 21KD. The expected band size for FGF21 is at 21KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-podoplanin-gp36-antibody-pa1675-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1675-pdpn-primary-antibodies-wb-testing-1.jpgAnti-Podoplanin/gp36/PDPN Antibody Picoband&reg; Western blot analysis of Podoplanin/gp36 using anti-Podoplanin/gp36 antibody (PA1675). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Podoplanin/gp36 antigen affinity purified polyclonal antibody (Catalog # PA1675) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Podoplanin/gp36 at approximately 38 kDa. The expected band size for Podoplanin/gp36 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nur77-antibody-pa1676-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1676-1-WB-anti-nur77-antibody.jpgAnti-NUR77/NR4A1 Antibody Picoband&reg;Anti-NUR77 antibody&#44; PA1676&#44; Western blotting<br>Lane 1: A431 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erp57-antibody-pa1679-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1679-1-WB-anti-erp57-antibody.jpgAnti-ERp57/PDIA3 Antibody Picoband&reg;Anti-ERp57 antibody&#44; PA1679&#44; Western blotting<br>Lane 1: SMMC Cell Lysate <br>Lane 2: A549 Cell Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: MCF-7 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1679-2-IHC-anti-erp57-antibody.jpgAnti-ERp57/PDIA3 Antibody Picoband&reg;Anti-ERp57 antibody&#44; PA1679&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pik3r2-antibody-pa1680-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1680-1_1.jpgAnti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband&reg; Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Testis tissue lysates&#44; <br> Lane 2: 293T whole cell lysates&#44; <br> Lane 3: HELA whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PA1680) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1680-2-IHC-anti-pik3r2-pi-3-kinase-p85-beta-antibody.jpgAnti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband&reg; IHC analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680). <br> PIK3R2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PIK3R2 Antibody (PA1680) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1680-3.jpgAnti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband&reg; Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PA1680) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 85KD. The expected band size for PIK3R2 is at 85KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppp1r12a-antibody-pa1681-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1681-1_2.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (PA1681). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44; <br> Lane 2: human Jurka7 whole cell lysates&#44; <br> Lane 3: human HEK293 whole cell lysates&#44; <br> Lane 4: monkey COS-7 whole cell lysates&#44; <br> Lane 5: human Raji whole cell lysates&#44; <br> Lane 6: human K562 whole cell lysates&#44; <br> Lane 7: human Caco-2 whole cell lysates&#44; <br> Lane 8: human HepG2 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (Catalog # PA1681) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 140KD. The expected band size for PPP1R12A is at 115KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1681-2.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (PA1681).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat C6 whole cell lysates&#44;<br> Lane 3: mouse liver tissue lysates&#44; <br> Lane 4: mouse NIH/3T3 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (Catalog # PA1681) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 140KD. The expected band size for PPP1R12A is at 115KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1681-ppp1r12a-primary-antibodies-if-testing-3.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; IF analysis of PPP1R12A using anti-PPP1R12A antibody (PA1681). <br> PPP1R12A was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PPP1R12A Antibody (PA1681) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-protein-c-antibody-pa1682-boster.html2026-03-24T05:03:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1682-1-WB-anti-protein-c-antibody.jpgAnti-Protein C/PROC Antibody Picoband&reg; Western blot analysis of Protein C using anti-Protein C antibody (PA1682). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: JURKAT Cell Lysate <br> Lane 2: CEM Cell Lysate<br> Lane 3: SMMC Cell Lysate<br> Lane 4: HELA Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Protein C antigen affinity purified polyclonal antibody (Catalog # PA1682) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Protein C at approximately 36KD. The expected band size for Protein C is at 52KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1682-2_1.jpgAnti-Protein C/PROC Antibody Picoband&reg; IHC analysis of Protein C using anti-Protein C antibody (PA1682).<br> Protein C was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Protein C Antibody (PA1682) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1682-proc-primary-antibodies-fc-testing-3.jpgAnti-Protein C/PROC Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-PROC antibody (PA1682). <br>Overlay histogram showing A549 cells stained with PA1682 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PROC Antibody (PA1682&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc22a6-antibody-pa1683-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1683-2-IHC-anti-slc22a6-oat1-antibody.jpgAnti-SLC22A6 Antibody Picoband&reg;Anti-SLC22A6 antibody&#44; PA1683&#44; IHC(P)<br>IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1683-1_1.jpgAnti-SLC22A6 Antibody Picoband&reg;Anti-SLC22A6 antibody&#44; PA1683&#44; Western blotting<br>Lane 1: HT1080 Cell Lysate <br>Lane 2: HELA Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1683-slc22a6-primary-antibodies-ihc-testing-4.jpgAnti-SLC22A6 Antibody Picoband&reg;IHC analysis of OAT1/SLC22A6 using anti-OAT1/SLC22A6 antibody (PA1683). <br>OAT1/SLC22A6 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OAT1/SLC22A6 Antibody (PA1683) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1683-3.jpgAnti-SLC22A6 Antibody Picoband&reg; IHC analysis of SLC22A6 using anti-SLC22A6 antibody (PA1683). <br> SLC22A6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC22A6 Antibody (PA1683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tacr1-antibody-pa1684-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1684-1_1.jpgAnti-Neurokinin 1 Receptor/TACR1 Antibody Picoband&reg;Western blot analysis of TACR1 using anti-TACR1antibody (PA1684).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A549 Whole Cell Lysate,<br> Lane 2: human U87 Whole Cell Lysate,<br> Lane 3: human COLO320 Whole Cell Lysate,<br> Lane 4: human SGC-7901 Whole Cell Lysate,<br> Lane 5: human PANC-1 Whole Cell Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PA1684) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55KD. The expected band size for TACR1 is at 45KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stra8-antibody-pa1685-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1685-1-WB-anti-stra8-antibody.jpgAnti-Stra8 Antibody Picoband&reg;Anti-Stra8 antibody&#44; PA1685&#44; Western blotting<br>Lane 1: A231 Cell Lysate<br>Lane 2: JURKAT Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br>Lane 4: SCG Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nlrp4g-antibody-pa1687-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1687-1-WB-anti-nlrp4g-antibody.jpgAnti-NLRP4G Antibody Picoband&reg;Anti-NLRP4G antibody&#44; PA1687&#44; Western blotting<br>WB: Mouse Ovary Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1687-nlrp4g-primary-antibodies-ihc-testing-2.jpgAnti-NLRP4G Antibody Picoband&reg; IHC analysis of NLRP4G using anti-NLRP4G antibody (PA1687). <br> NLRP4G was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NLRP4G Antibody (PA1687) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-estrogen-inducible-protein-ps2-antibody-pa1689-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1689-tff1-primary-antibodies-wb-testing-1.jpgAnti-Estrogen Inducible Protein pS2/TFF1 Antibody Picoband&reg; Western blot analysis of TFF1 using anti-TFF1 antibody (PA1689). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFF1 antigen affinity purified polyclonal antibody (Catalog # PA1689) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFF1 at approximately 12 kDa. The expected band size for TFF1 is at 9 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1689-1-IHC-anti-tff1-ps2-antibody.jpgAnti-Estrogen Inducible Protein pS2/TFF1 Antibody Picoband&reg;Anti-Estrogen Inducible Protein Ps2 antibody&#44; PA1689&#44; IHC(P)<br>IHC(P): Human Gastric Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-estrogen-inducible-protein-ps2-antibody-pa1690-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1690-1-WB-anti-tff1-ps2-antibody.jpgAnti-Estrogen Inducible Protein pS2/TFF1 Antibody Picoband&reg;Anti-Estrogen Inducible Protein pS2 antibody&#44; PA1690&#44; Western blotting<br>WB: Rat Stomach Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1690-2-IHC-anti-tff1-ps2-antibody.jpgAnti-Estrogen Inducible Protein pS2/TFF1 Antibody Picoband&reg;Anti-Estrogen Inducible Protein pS2 antibody&#44; PA1690&#44; IHC(P)<br>IHC(P): Rat Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat6-antibody-pa1691-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1691-stat6-primary-antibodies-wb-testing-1.jpgAnti-STAT6 Antibody Picoband&reg;Western blot analysis of STAT6 using anti-STAT6 antibody (PA1691). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: mouse thymus tissue lysates, <br> Lane 4: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 antigen affinity purified polyclonal antibody (PA1691) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT6 at approximately 100 kDa. The expected band size for STAT6 is at 94 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1691-stat6-primary-antibodies-ihc-testing-1.jpgAnti-STAT6 Antibody Picoband&reg;IHC analysis of STAT6 using anti-STAT6 antibody (PA1691). <br> STAT6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT6 Antibody (PA1691) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1691-stat6-primary-antibodies-if-testing-1.jpgAnti-STAT6 Antibody Picoband&reg;IF analysis of STAT6 using anti-STAT6 antibody (PA1691) and anti-Tubulin Alpha antibody (M03989-3).<br> STAT6 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAT6 Antibody (PA1691) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1691-stat6-primary-antibodies-fcm-testing-1.jpgAnti-STAT6 Antibody Picoband&reg;Flow Cytometry analysis of Jurkat cells using anti-STAT6 antibody (PA1691). <br> Overlay histogram showing Jurkat cells stained with PA1691 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT6 Antibody (PA1691, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat4-antibody-pa1692-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1692-fcimb-14-1416105-g004.jpgAnti-STAT4 Antibody Picoband&reg;NLRP12 enhances antiviral functions through IL-18 downstream JAK-STAT signaling. (A) Protein levels of STAT1, p-STAT1, STAT4, pSTAT4 were assessed at 8h, 12h and 24h post-infection in both groups. Western blot bands were normalized to β-actin and compared against controls (n=3 per group). (B) Immunofluorescence images demonstrating MHC II + cell staining. All of the data are representative of at least three independent experiments. Data are presented as the mean ± SEM. Statistical differences were determined using two-way ANOVA (A) . ns, not significnat, *P <0.05, ***P <0.001, ****P <0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11306119/'>39119293</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1692-stat4-primary-antibodies-wb-testing-1.jpgAnti-STAT4 Antibody Picoband&reg;Western blot analysis of STAT4 using anti-STAT4 antibody (PA1692). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human HEL whole cell lysates,<br> Lane 4: rat testis tissue lysates,<br> Lane 5: mouse testis tissue lysates,<br> Lane 6: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT4 antigen affinity purified polyclonal antibody (PA1692) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT4 at approximately 86 kDa. The expected band size for STAT4 is at 86 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-somatostatin-receptor-1-antibody-pa1693-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1693-1-WB-anti-somatostatin-receptor-1-antibody.jpgAnti-Somatostatin Receptor 1/SSTR1 Antibody Picoband&reg;Anti-SSTR1 antibody&#44; PA1693&#44; Western blotting<br>All lanes: Anti SSTR1 (PA1693) at 0.5ug/ml<br>WB: Rat Intestine Tissue Lysate at 50ug<br>Predicted bind size: 43KD<br>Observed bind size: 60KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1693-2-IHC-anti-somatostatin-receptor-1-antibody.jpgAnti-Somatostatin Receptor 1/SSTR1 Antibody Picoband&reg;Anti-SSTR1 antibody&#44; PA1693&#44; IHC(P)<br>IHC(P): Rat Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnr-antibody-pa1695-1-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-1-tnr-primary-antibodies-wb-testing-1.jpgAnti-Tenascin-R TNR Antibody Picoband&reg;Western blot analysis of TNR using anti-TNR antibody (PA1695-1). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNR antigen affinity purified polyclonal antibody (PA1695-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNR at approximately 180-250 kDa. The expected band size for TNR is at 150 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-1-tnr-primary-antibodies-ihc-testing-1.jpgAnti-Tenascin-R TNR Antibody Picoband&reg;IHC analysis of TNR using anti-TNR antibody (PA1695-1). <br> TNR was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNR Antibody (PA1695-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-1-tnr-primary-antibodies-ihc-testing-2.jpgAnti-Tenascin-R TNR Antibody Picoband&reg;IHC analysis of TNR using anti-TNR antibody (PA1695-1). <br> TNR was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNR Antibody (PA1695-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-1-tnr-primary-antibodies-ihc-testing-3.jpgAnti-Tenascin-R TNR Antibody Picoband&reg;IHC analysis of TNR using anti-TNR antibody (PA1695-1). <br> TNR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNR Antibody (PA1695-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-1-tnr-primary-antibodies-ihc-testing-4.jpgAnti-Tenascin-R TNR Antibody Picoband&reg;IHC analysis of TNR using anti-TNR antibody (PA1695-1). <br> TNR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNR Antibody (PA1695-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-1-tnr-primary-antibodies-fcm-testing-5.jpgAnti-Tenascin-R TNR Antibody Picoband&reg;Flow Cytometry analysis of SH-SY5Y cells using anti-TNR antibody (PA1695-1). <br> Overlay histogram showing SH-SY5Y cells stained with PA1695-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNR Antibody (PA1695-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xrcc3-antibody-pa1697-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1697-1_1-WB-anti-xrcc3-antibody.jpgAnti-DNA repair protein XRCC3 XRCC3 Antibody Picoband&reg; Western blot analysis of -XRCC3 using anti--XRCC3 antibody (PA1697).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Mouse Brain Tissue Lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--XRCC3 antigen affinity purified polyclonal antibody (Catalog # PA1697) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -XRCC3 at approximately 38KD. The expected band size for -XRCC3 is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1697-2-IHC-anti-xrcc3-antibody.jpgAnti-DNA repair protein XRCC3 XRCC3 Antibody Picoband&reg; IHC analysis of XRCC3 using anti-XRCC3 antibody (PA1697).<br> XRCC3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-XRCC3 Antibody (PA1697) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp11a1-antibody-pa1698-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1698-1-WB-anti-cyp11a1-antibody.jpgAnti-CYP11A1 Antibody Picoband&reg;Anti-CYP11A1 antibody&#44; PA1698&#44; Western blotting<br>WB: Human Placenta Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1698-2-IHC-anti-cyp11a1-antibody.jpgAnti-CYP11A1 Antibody Picoband&reg;Anti-CYP11A1 antibody&#44; PA1698&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1698-3-IHC-anti-cyp11a1-antibody.jpgAnti-CYP11A1 Antibody Picoband&reg;Anti-CYP11A1 antibody&#44; PA1698&#44; IHC(F)<br>IHC(F): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp11a1-antibody-pa1698-1-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1698-1-1-WB-anti-cyp11a1-antibody.jpgAnti-CYP11A1 Antibody Picoband&reg;Anti-CYP11A1 antibody&#44; PA1698-1&#44; Western blotting<br>All lanes: Anti CYP11A1 (PA1698-1) at 0.5ug/ml<br>Lane 1: Rat Testis Tissue Lysate at 50ug<br>Lane 2: Mouse Testis Tissue Lysate at 50ug<br>Lane 3: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 60KD<br>Observed bind size: 60KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp11b1-antibody-pa1699-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1699-1-WB-anti-cyp11b1-antibody.jpgAnti-CYP11B1 Antibody Picoband&reg;Anti-CYP11B1 antibody&#44; PA1699&#44; Western blotting<br>All lanes: Anti CYP11B1 (PA1699) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: U87 Whole Cell Lysate at 40ug<br> Lane 3: MM231 Whole Cell Lysate at 40ug<br> Lane 4: PANC Whole Cell Lysate at 40ug<br> Lane 5: MM453 Whole Cell Lysate at 40ug<br> Lane 6: HELA Whole Cell Lysate at 40ug<br> Lane 7: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 58KD<br> Observed bind size: 58KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-6-antibody-pa1700-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1700-il6-primary-antibodies-wb-testing-1.jpgAnti-Interleukin-6 IL6 Antibody Picoband&reg;Western blot analysis of IL6 using anti-IL6 antibody (PA1700). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human IL6 protein 5 ng,<br> Lane 2: recombinant human IL6 protein 2.5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (PA1700) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL6 at approximately 24 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-13-antibody-pa1701-boster.html2026-03-24T05:03:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1701-1-WB-anti-il-13-antibody.jpgAnti-Interleukin-13 IL13 Antibody Picoband&reg;Anti-IL-13 antibody&#44; PA1701&#44; Western blotting<br>Lane 1: Recombinant Human IL-13 Protein 10ng<br>Lane 2: Recombinant Human IL-13 Protein 5ng<br>Lane 3: Recombinant Human IL-13 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc6a4-antibody-pa1705-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1705-1-WB-anti-slc6a4-serotonin-transporter-antibody.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg;Anti-SLC6A4 antibody&#44; PA1705&#44; Western blotting<br>All lanes: Anti SLC6A4 (PA1705) at 0.5ug/ml<br> Lane 1: U87 Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: JURKAT Whole Cell Lysate at 40ug<br> Predicted bind size: 70KD<br> Observed bind size: 70KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc6a4-antibody-pa1706-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1706-1-IHC-anti-slc6a4-serotonin-transporter-antibody.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg;Anti-SLC6A4 antibody&#44; PA1706&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1706-2-WB-anti-slc6a4-serotonin-transporter-antibody.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg;Anti-SLC6A4 antibody&#44; PA1706&#44; Western blotting<br>WB: Rat Brain Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-socs3-antibody-pa1707-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1707-1_2.jpgAnti-SOCS3 Antibody Picoband&reg; Western blot analysis of SOCS3 using anti-SOCS3 antibody (PA1707). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44; <br> Lane 2: human A549 whole cell lysates&#44; <br> Lane 3: human A431 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody (Catalog # PA1707) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 30KD. The expected band size for SOCS3 is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1707-2-IHC-anti-socs3-antibody.jpgAnti-SOCS3 Antibody Picoband&reg; IHC analysis of SOCS3 using anti-SOCS3 antibody (PA1707). <br> SOCS3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOCS3 Antibody (PA1707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-1-antibody-pa1709-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1709-1-WB-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Anti-Kallikrein 1 antibody&#44; PA1709&#44; Western blotting<br>Lane 1: Recombinant Mouse KLK1 Protein 10ng<br>Lane 2: Recombinant Mouse KLK1 Protein 5ng<br>Lane 3: Recombinant Mouse KLK1 Protein 2.5ng<br>Lane 4: Recombinant Mouse KLK1 Protein 1.25nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1709-2-IHC-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Anti-Kallikrein 1 antibody&#44; PA1709&#44; IHC(P)<br>IHC(P): Mouse Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1709-ajtr0016-0544-f5.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Western blot and densitometric analysis of various proteases in kidney lysates from sham (SH) and unilateral nephrectomized (UNX) mice. A. Western blot (top) of kidney kallikrein 1 (KLK1) protein expression in kidney lysates from SH and UNX mice. Densitometric analysis (bottom) of the immunoreactive band corresponding to KLK1. B. Western blot (top) of cathepsin B (Cat-B) protein expression in kidney lysates from SH and UNX mice. Densitometric analysis (bottom) of the immunoreactive band corresponding to cathepsin B. C. Western blot (top) of cathepsin D (Cat-D) protein expression in kidney lysates from SH and UNX mice. Densitometric analysis (bottom) of the immunoreactive band corresponding to cathepsin D. D. Western blot (top) of cathepsin S (Cat-S) protein expression in kidney lysates from SH and UNX mice. Densitometric analysis (bottom) of the immunoreactive band corresponding to cathepsin S. *P < 0.05. N=4 mice per group. A Student t-test was used to compare the differences between the two groups. N=4 samples per group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10918144/&orig_host=www.ncbi.nlm.nih.gov'>38463588</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1709-ajtr0016-0544-f6.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Immunohistochemistry (IHC) of KLK1 and Cathepsin isoform expression in the kidney from sham (SH) and unilateral nephrectomized (UNX) mice. A. IHC of kidney kallikrein 1 (KLK1) protein expression in kidney lysates from SH and UNX mice. B. IHC of kidney cathepsin B (Cat-B) protein expression in kidney lysates from SH and UNX mice. C. IHC of kidney cathepsin S (Cat-S) protein expression in kidney lysates from SH and UNX mice. D. IHC of kidney cathepsin D (Cat-D) protein expression in kidney lysates from SH and UNX mice. 40X magnification. N=4 mice per group. The scale bar denotes 500 µM. Images are at 40X magnification.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10918144/&orig_host=www.ncbi.nlm.nih.gov'>38463588</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1709-41419_2014_article_bfcddis2014428_fig4_html.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Acinar cell apoptosis is delayed in post-ligated SMGs of ATG5-deficient mice. ( a ) ATG5 status impinges upon duct ligation-triggered acinar apoptosis. Apoptosis by ApopTag Peroxidase (brown nuclear staining) was visualized using an In situ Apoptosis Detection Kit ( left panel ). Peak apoptosis was detected in L1 and L3 SMGs of Atg5 WT mice, whereas the strongest ApopTag signals were detected in L3 and L7 SMGs of Atg5 KO mice. Magnification: × 100, and enlarged view ( inset ): × 400. Bar: 100 μ m. Percent ApopTag-positive cells quantified by dividing the total ApopTag-positive cells by total number of cells examined from 10 randomly chosen fields are shown ( right panel ). Student’s t -test was employed to determine statistically significant differences in percentage of ApopTag-positive cells between Atg5 WT and Atg5 KO groups. Results are shown as mean±S.D.; * P <0.05. ( b ) ATG5 deficiency delays caspase-3 activation. Equal amounts of whole SMG lysates from Ctrl, L1, L3 and L7 SMGs of Atg5 WT and Atg5 KO mice were analyzed on western blots using indicated antibodies. The cleavage of caspase-3 represents caspase-3 activation. ( c ) Gene expression analyses of Prol1/Muc-10 and Klk1 , markers for acinar and duct cells, respectively. SMG duct ligation was performed as described in . Expression of the indicated messages in ligated and control SMGs was analyzed by quantitative RT-PCR analyses. Relative gene expression was calculated where respective mean value for control SMG set to 1 for each amplification ( N ≥4). Non-parametric Mann–Whitney test was performed to compare expression levels between respective ligated and control SMGs ( *; below bars), and between same-day ligated SMGs and control SMGs from Atg5 WT and Atg5 KO ( *; above bars). Results are shown as mean±S.D.; * P <0.05; ** P <0.01. ( d ) Autophagy inhibition correlates with resistance to H 2 O 2 -induced cell death. Cell viability was measured in m5-7, Atg7 -KO MEF, WT MEF and salivary Pa-4 cells treated with indicated concentrations of H 2 O 2 in combination with chloroquine (CQ; 20 μ M) or baflomycin A1 (BafA1; 10 nM) for 48 h and compared with that of the control cells. Data were analyzed with ANOVA followed by Bonferroni t -test to determine the statistical differences between treatment and control group ( left panels ). Representative western blots ( right panels ), in which total lysates from cells treated for 6 h with vehicle, CQ (20 μ M) or BafA1 (10 nM) were stained with indicated antibodies, are shown. Results are shown as mean±S.E.M.; N =3; * P <0.05; ** P <0.01; *** P <0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis2014428'>25341032</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1709-3.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg; Western blot analysis of KLK1 using anti-KLK1 antibody (PA1709). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat pancreas tissue lysates&#44; <br> Lane 2: mouse pancreas tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLK1 antigen affinity purified polyclonal antibody (Catalog # PA1709) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLK1 at approximately 29KD. The expected band size for KLK1 is at 29KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm2-antibody-pa1711-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1711-1-IHC-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1711&#44; IHC(P)<br>IHC(P): Rat Intestinal Lymphocyte Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1711-2-IHC-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1711&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1711-3-WB-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1711&#44; Western blotting<br>All lanes: Anti MCM2 (PA1711) at 0.5ug/ml<br> Lane 1: Rat Testis Tissue Lysate at 50ug<br> Lane 2: Rat Kidney Tissue Lysate at 50ug<br> Lane 3: Mouse Intestine Tissue Lysate at 50ug<br> Lane 4: Mouse Testis Tissue Lysate at 50ug<br> Lane 5: Mouse Ovary Tissue Lysate at 50ug<br> Lane 6: Mouse Kidney Tissue Lysate at 50ug<br> Predicted bind size: 102KD<br> Observed bind size: 102KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1711-4-IF-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1711&#44; ICC<br>ICC: HEPA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1711-5-IF-anti-mcm2-antibody.jpgAnti-MCM2 Antibody Picoband&reg;Anti-MCM2 antibody&#44; PA1711&#44; ICC<br>ICC: NIH3T3 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1711-mcm2-primary-antibody-if-testing-6.jpgAnti-MCM2 Antibody Picoband&reg; IF analysis of MCM2 using anti-MCM2 antibody (PA1711). <br> MCM2 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MCM2 Antibody (PA1711) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1711-mcm2-primary-antibody-fcm-testing-7.pngAnti-MCM2 Antibody Picoband&reg; Flow Cytometry analysis of Neuro-2a cells using anti-MCM2 antibody (PA1711). <br>Overlay histogram showing Neuro-2a cells stained with PA1711 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM2Antibody (PA1711, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pbp-antibody-pa1713-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1713-pebp1-primary-antibodies-wb-testing-1.jpgAnti-PBP/PEBP1 Antibody Picoband&reg; Western blot analysis of PEBP1 using anti-PEBP1 antibody (PA1713). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse brian tissue lysates, <br> Lane 4: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEBP1 antigen affinity purified polyclonal antibody (Catalog # PA1713) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEBP1 at approximately 21 kDa. The expected band size for PEBP1 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1713-2-IHC-anti-pbp-antibody.jpgAnti-PBP/PEBP1 Antibody Picoband&reg; IHC analysis of PEBP1 using anti-PEBP1 antibody (PA1713). <br> PEBP1 was detected in a paraffin-embedded section of Rat Kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PEBP1 Antibody (PA1713) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd62p-antibody-pa1715-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1715-1-WB-anti-p-selectin-antibody.jpgAnti-CD62P/SELP Antibody Picoband&reg;Anti-CD62P antibody&#44; PA1715&#44; Western blotting<br>All lanes: Anti CD62P (PA1715) at 0.5ug/ml<br>WB: PANC Whole Cell Lysate at 40ug<br>Predicted bind size: 91KD<br>Observed bind size: 140KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1715-3_1-IHC-anti-p-selectin-antibody.jpgAnti-CD62P/SELP Antibody Picoband&reg;Anti-CD62P antibody&#44; PA1715&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1715-2_1-IHC-anti-p-selectin-antibody.jpgAnti-CD62P/SELP Antibody Picoband&reg;Anti-CD62P antibody&#44; PA1715&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rfc1-antibody-pa1716-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1716-rfc1-primary-antibodies-wb-testing-1.jpgAnti-Replication factor C subunit 1 RFC1 Antibody Picoband&reg; Western blot analysis of RFC1 using anti-RFC1 antibody (PA1716). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human Raji whole cell lysates,<br> Lane 5: human K562 whole cell lysates,<br> Lane 6: human CACO-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC1 antigen affinity purified polyclonal antibody (Catalog # PA1716) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC1 at approximately 150 kDa. The expected band size for RFC1 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1716-2-IHC-anti-rfc1-antibody.jpgAnti-Replication factor C subunit 1 RFC1 Antibody Picoband&reg; IHC analysis of RFC1 using anti-RFC1 antibody (PA1716).<br> RFC1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RFC1 Antibody (PA1716) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1716-rfc1-primary-antibodies-if-testing-3.jpgAnti-Replication factor C subunit 1 RFC1 Antibody Picoband&reg; IF analysis of RFC1 using anti-RFC1 antibody (PA1716) and anti-Beta Tubulin antibody (M01857-3).<br> RFC1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RFC1 Antibody (PA1716) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1716-rfc1-primary-antibodies-fcm-testing-4.jpgAnti-Replication factor C subunit 1 RFC1 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-RFC1 antibody (PA1716). <br> Overlay histogram showing Hela cells stained with PA1716 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RFC1 Antibody (PA1716, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sdha-antibody-pa1717-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1717-sdha-primary-antibodies-wb-testing-1_1.jpgAnti-SDHA Antibody Picoband&reg; Western blot analysis of SDHA using anti-SDHA antibody (PA1717). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: mouse brain tissue lysates, <br> Lane 6: mouse spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHA antigen affinity purified polyclonal antibody (Catalog # PA1717) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHA at approximately 73 kDa. The expected band size for SDHA is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1717-sdha-primary-antibodies-ihc-testing-2.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PA1717). <br> SDHA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PA1717) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1717-sdha-primary-antibodies-ihc-testing-3.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PA1717). <br> SDHA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PA1717) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1717-sdha-primary-antibodies-ihc-testing-4.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PA1717). <br> SDHA was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PA1717) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1717-sdha-primary-antibodies-ihc-testing-5.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PA1717). <br> SDHA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PA1717) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1717-sdha-primary-antibodies-ihc-testing-6.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PA1717). <br> SDHA was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PA1717) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1717-sdha-primary-antibodies-fcm-testing-7.jpgAnti-SDHA Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-SDHA antibody (PA1717). <br> Overlay histogram showing PC-3 cells stained with PA1717 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (PA1717, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sdhb-antibody-pa1718-boster.html2026-03-24T05:03:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1718-sdhb-primary-antibodies-wb-testing-1.jpgAnti-SDHB Antibody Picoband&reg; Western blot analysis of SDHB using anti-SDHB antibody (PA1718). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: rat heart tissue lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: mouse heart tissue lysates, <br> Lane 6: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHB antigen affinity purified polyclonal antibody (Catalog # PA1718) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHB at approximately 29 kDa. The expected band size for SDHB is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1718-sdhb-primary-antibodies-ihc-testing-2.jpgAnti-SDHB Antibody Picoband&reg; IHC analysis of SDHB using anti-SDHB antibody (PA1718). <br> SDHB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (PA1718) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1718-sdhb-primary-antibodies-ip-testing-1.jpgAnti-SDHB Antibody Picoband&reg;Immunoprecipitating (IP) SDHB in HepG2 whole cell lysate.<br> Western blot analysis of SDHB using anti-SDHB antibody (PA1718); <br> Lane 1: HepG2 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-SDHB antibody in HepG2 whole cell lysate;<br> Lane 3: anti-SDHB antibody (2μg) + HepG2 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SDHB antigen affinity purified polyclonal antibody (PA1718) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SDHB at approximately 29 kDa. The expected band size for SDHB is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1718-sdhb-primary-antibodies-ihc-testing-3.jpgAnti-SDHB Antibody Picoband&reg; IHC analysis of SDHB using anti-SDHB antibody (PA1718). <br> SDHB was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHB Antibody (PA1718) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serca1-atpase-antibody-pa1719-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1719-1-WB-anti-serca1-atpase-antibody.jpgAnti-SERCA1 ATPase/ATP2A1 Antibody Picoband&reg;Anti-SERCA1 ATPase antibody&#44; PA1719&#44; Western blotting<br>Lane 1: Rat Skeletal Muscle Tissue Lysate <br>Lane 2: PANC Cell Lysate <br>Lane 3: U87 Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1719-2-IHC-anti-serca1-atpase-antibody.jpgAnti-SERCA1 ATPase/ATP2A1 Antibody Picoband&reg;Anti-SERCA1 ATPase antibody&#44; PA1719&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1719-3-IHC-anti-serca1-atpase-antibody.jpgAnti-SERCA1 ATPase/ATP2A1 Antibody Picoband&reg;Anti-SERCA1 ATPase antibody&#44; PA1719&#44; IHC(P)<br>IHC(P): Rat Skeletal Muscle Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glut4-antibody-pa1722-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1722-1-IHC-anti-glut4-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg;Anti-GLUT4 antibody&#44; PA1722&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1722-2-WB-anti-glut4-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg;Anti-GLUT4 antibody&#44; PA1722&#44; Western blotting<br>All lanes: Anti GLUT4 (PA1722) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug<br>Lane 5: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 55 KD<br>Observed bind size: 55 KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1722-3.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg; IHC analysis of GLUT4 using anti-GLUT4 antibody (PA1722). <br> GLUT4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PA1722) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcb6-antibody-pa1723-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1723-1-WB-anti-abcb6-antibody.jpgAnti-ABCB6 Antibody Picoband&reg;Anti-ABCB6 antibody&#44; PA1723&#44; Western blotting<br>All lanes: Anti ABCB6 (PA1723) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Lane 3: A549 Whole Cell Lysate at 40ug<br> Predicted bind size: 94KD<br> Observed bind size: 94KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1723-2-IHC-anti-abcb6-antibody.jpgAnti-ABCB6 Antibody Picoband&reg;Anti-ABCB6 antibody&#44; PA1723&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cox2-cyclooxygenase-2-antibody-pa1725-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1725-ptgs2-primary-antibodies-wb-testing-1.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Antibody Picoband&reg; Western blot analysis of PTGS2 using anti-PTGS2 antibody (PA1725). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: mouse thymus tissue lysates, <br> Lane 3: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGS2 antigen affinity purified polyclonal antibody (Catalog # PA1725) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGS2 at approximately 75 kDa. The expected band size for PTGS2 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1725-ptgs2-primary-antibodies-ihc-testing-2.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA1725). <br> SOX2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA1725) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1725-ptgs2-primary-antibodies-ihc-testing-3.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA1725). <br> SOX2 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA1725) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1725-ptgs2-primary-antibodies-ihc-testing-4.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA1725). <br> SOX2 was detected in a paraffin-embedded section of human differentiated adenocarcinoma in the appendix-1 tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA1725) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1725-ptgs2-primary-antibodies-ihc-testing-5.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA1725). <br> SOX2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA1725) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1725-ptgs2-primary-antibodies-ihc-testing-6.jpgAnti-COX2/Cyclooxygenase 2/PTGS2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA1725). <br> SOX2 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA1725) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-growth-hormone-receptor-antibody-pa1726-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1726-1-WB-anti-ghr-growth-hormone-r-antibody.jpgAnti-Growth hormone receptor/GHR Antibody Picoband&reg;Anti-GHR antibody&#44; PA1726&#44; Western blotting<br>All lanes: Anti GHR (PA1726) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: Rat Kidney Tissue Lysate at 50ug<br>Lane 3: Rat Spleen Tissue Lysate at 50ug<br>Lane 4: Rat Intestine Tissue Lysate at 50ug<br>Lane 5: Mouse Spleen Tissue Lysate at 50ug<br>Lane 6: Mouse Testis Tissue Lysate at 50ug<br>Lane 7: Mouse Liver Tissue Lysate at 50ug<br>Lane 8: Mouse Kidney Tissue Lysate at 50ug<br>Lane 9: Mouse Intestine Tissue Lysate at 50ug<br>Predicted bind size: 72KD<br>Observed bind size: 72KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hydroxysteroid-17-beta-dehydrogenase-4-antibody-pa1727-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1727-hsd17b4-primary-antibodies-wb-testing-1.jpgAnti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband&reg; Western blot analysis of HSD17B4 using anti-HSD17B4 antibody (PA1727). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B4 antigen affinity purified polyclonal antibody (Catalog # PA1727) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B4 at approximately 80 kDa. The expected band size for HSD17B4 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1727-2-IHC-anti-hydroxysteroid-17-beta-dehydrogenase-4-antibody.jpgAnti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband&reg; IHC analysis of HSD17B4 using anti-HSD17B4 antibody (PA1727). <br> HSD17B4 was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B4 Antibody (PA1727) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1727-3-IHC-anti-hydroxysteroid-17-beta-dehydrogenase-4-antibody.jpgAnti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband&reg; IHC analysis of HSD17B4 using anti-HSD17B4 antibody (PA1727). <br> HSD17B4 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD17B4 Antibody (PA1727) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-3-antibody-pa1728-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1728-1-WB-anti-il-3-antibody.jpgAnti-Interleukin-3 IL3 Antibody Picoband&reg;Anti-IL-3 antibody&#44; PA1728&#44; Western blotting<br>All lanes: Anti IL-3 (PA1728) at 0.5ug/ml<br>Lane 1: Recombinant Mouse IL-3 Protein 10ng<br>Lane 2: Recombinant Mouse IL-3 Protein 5ng<br>Lane 3: Recombinant Mouse IL-3 Protein 2.5ng<br>Predicted bind size: 14KD<br>Observed bind size: 14KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pak6-antibody-pa1729-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1729-pak6-primary-antibodies-wb-testing-1.jpgAnti-PAK6 Antibody Picoband&reg;Western blot analysis of PAK6 using anti-PAK6 antibody (PA1729). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human CACO-2 whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates,<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK6 antigen affinity purified polyclonal antibody (PA1729) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK6 at approximately 70-75 kDa. The expected band size for PAK6 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1729-pak6-primary-antibodies-if-testing-1.jpgAnti-PAK6 Antibody Picoband&reg;IF analysis of PAK6 using anti-PAK6 antibody (PA1729) and anti-Tubulin Alpha antibody (M03989-3).<br> PAK6 was detected in immunocytochemical section of CACO-2 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PAK6 Antibody (PA1729) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1729-pak6-primary-antibodies-fcm-testing-1.jpgAnti-PAK6 Antibody Picoband&reg;Flow Cytometry analysis of CACO-2 cells using anti-PAK6 antibody (PA1729). <br> Overlay histogram showing CACO-2 cells stained with PA1729 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAK6 Antibody (PA1729, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr7-antibody-pa1733-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1733-1-WB-anti-tlr7-antibody.jpgAnti-Toll-like receptor 7 TLR7 Antibody Picoband&reg;Anti-TLR7 antibody&#44; PA1733&#44; Western blotting<br>Lane 1: Rat Spleen Tissue Lysate<br>Lane 2: Rat Liver Tissue Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: A549 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1733-2-IHC-anti-tlr7-antibody.jpgAnti-Toll-like receptor 7 TLR7 Antibody Picoband&reg;Anti-TLR7 antibody&#44; PA1733&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1733-3-IHC-anti-tlr7-antibody.jpgAnti-Toll-like receptor 7 TLR7 Antibody Picoband&reg;Anti-TLR7 antibody&#44; PA1733&#44; IHC(P)<br>IHC(P): Rat Spleen Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr8-antibody-pa1734-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1734-tlr8-primary-antibodies-wb-testing-1.jpgAnti-Toll-like receptor 8 TLR8 Antibody Picoband&reg; Western blot analysis of TLR8 using anti-TLR8 antibody (PA1734). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR8 antigen affinity purified polyclonal antibody (Catalog # PA1734) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR8 at approximately 150 kDa. The expected band size for TLR8 is at 120 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-8-p18-antibody-pa1735-boster.html2026-03-24T05:03:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1735-1-WB-anti-caspase-8-p18-antibody.jpgAnti-Caspase-8(p18)/CASP8 Antibody Picoband&reg;Anti-Caspase-8(p18) antibody&#44; PA1735&#44; Western blotting<br>All lanes: Anti Caspase-8(p18) (PA1735) at 0.5ug/ml<br>Lane 1: A549 Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 18KD<br>Observed bind size: 18KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1735-2-IHC-anti-caspase-8-p18-antibody.jpgAnti-Caspase-8(p18)/CASP8 Antibody Picoband&reg;Anti-Caspase-8(p18) antibody&#44; PA1735&#44;IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc2a5-antibody-pa1737-boster.html2026-03-28T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1737-slc2a5-primary-antibodies-wb-testing-1.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg; Western blot analysis of GLUT5/SLC2A5 using anti-GLUT5/SLC2A5 antibody (PA1737). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: mouse testis tissue lysates,<br> Lane 4: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT5/SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PA1737) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT5/SLC2A5 at approximately 50 kDa. The expected band size for GLUT5/SLC2A5 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1737-2-IHC-anti-slc2a5-glut5-antibody.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg;Anti-SLC2A5 antibody&#44; PA1737&#44; IHC(P)<br>IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1737-3-IHC-anti-slc2a5-glut5-antibody.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg;Anti-SLC2A5 antibody&#44; PA1737&#44; IHC(F)<br>IHC(F): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1737-4.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg; IHC analysis of GLUT5 using anti-GLUT5 antibody (PA1737). <br> GLUT5 was detected in paraffin-embedded section of mouse ggj3249 tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT5 Antibody (PA1737) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-granzyme-b-antibody-pa1738-boster.html2026-03-27T05:07:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1738-gzmb-primary-antibodies-wb-testing-1.jpgAnti-Granzyme B/GZMB Antibody Picoband&reg;Western blot analysis of GZMB using anti-GZMB antibody (PA1738). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates,<br> Lane 2: rat spleen tissue lysates,<br> Lane 3: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GZMB antigen affinity purified polyclonal antibody (PA1738) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GZMB at approximately 33 kDa. The expected band size for GZMB is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1738-gzmb-primary-antibodies-wb-testing-2.jpgAnti-Granzyme B/GZMB Antibody Picoband&reg;Western blot analysis of GZMB using anti-GZMB antibody (PA1738). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human NK92 whole cell lysates,<br> Lane 2: human NK92 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GZMB antigen affinity purified polyclonal antibody (PA1738) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GZMB at approximately 33 kDa. The expected band size for GZMB is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1738-gzmb-primary-antibodies-ihc-testing-1.jpgAnti-Granzyme B/GZMB Antibody Picoband&reg;IHC analysis of GZMB using anti-GZMB antibody (PA1738). <br>GZMB was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GZMB Antibody (PA1738) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1738-gzmb-primary-antibodies-ihc-testing-2.jpgAnti-Granzyme B/GZMB Antibody Picoband&reg;IHC analysis of GZMB using anti-GZMB antibody (PA1738). <br>GZMB was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GZMB Antibody (PA1738) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1738-gzmb-primary-antibodies-ihc-testing-3.jpgAnti-Granzyme B/GZMB Antibody Picoband&reg;IHC analysis of GZMB using anti-GZMB antibody (PA1738). <br>GZMB was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GZMB Antibody (PA1738) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1738-gzmb-primary-antibodies-ihc-testing-4.jpgAnti-Granzyme B/GZMB Antibody Picoband&reg;IHC analysis of GZMB using anti-GZMB antibody (PA1738). <br>GZMB was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GZMB Antibody (PA1738) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nox1-antibody-pa1739-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1739-1-IHC-anti-nox1-antibody.jpgAnti-NADPH oxidase 1 NOX1 Antibody Picoband&reg;Anti-NOX1 antibody&#44; PA1739&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1739-2-WB-anti-nox1-antibody.jpgAnti-NADPH oxidase 1 NOX1 Antibody Picoband&reg;Anti-NOX1 antibody&#44; PA1739&#44; Western blotting<br>All lanes: Anti NOX1 (PA1739) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 65KD<br> Observed bind size: 65KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alk-antibody-pa1741-boster.html2026-03-29T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1741-alk-primary-antibodies-wb-testing-1.jpgAnti-ALK Antibody Picoband&reg;Western blot analysis of ALK using anti-ALK antibody (PA1741). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALK antigen affinity purified polyclonal antibody (PA1741) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALK at approximately 250 kDa. The expected band size for ALK is at 176 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1741-alk-primary-antibodies-ihc-testing-1.jpgAnti-ALK Antibody Picoband&reg;IHC analysis of ALK using anti-ALK antibody (PA1741). <br>ALK was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALK Antibody (PA1741) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1741-alk-primary-antibodies-ihc-testing-2.jpgAnti-ALK Antibody Picoband&reg;IHC analysis of ALK using anti-ALK antibody (PA1741). <br>ALK was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALK Antibody (PA1741) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-2-antibody-pa1742-boster.html2026-04-01T05:01:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1742-1-IHC-anti-aquaporin-2-antibody.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg;Anti-Aquaporin 2 antibody&#44; PA1742&#44; IHC(P)<br>IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1742-2-WB-anti-aquaporin-2-antibody.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg;Anti-Aquaporin 2 antibody&#44; PA1742&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: SW620 Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1742-3-IHC-anti-aquaporin-2-antibody.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg;Anti-Aquaporin 2 antibody&#44; PA1742&#44; IHC(F)<br>IHC(F): Rat Kidney Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd2-antibody-pa1743-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1743-1-WB-anti-cd2-antibody.jpgAnti-CD2/CD2 Antibody Picoband&reg;Anti-CD2 antibody&#44; PA1743&#44; Western blotting<br>Lane 1: Rat Spleen Cell Lysate<br>Lane 2: NIH/3T3 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igf1-receptor-antibody-pa1746-boster.html2026-04-01T05:01:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1746-igf1r-primary-antibodies-wb-testing-1.jpgAnti-IGF1 Receptor/IGF1R Antibody Picoband&reg;Western blot analysis of IGF1R using anti-IGF1R antibody (PA1746). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human SH-SY5Y whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF1R antigen affinity purified polyclonal antibody (PA1746) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGF1R at approximately 110, 200 kDa. The expected band size for IGF1R is at 155 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1746-igf1r-primary-antibodies-ihc-testing-2.jpgAnti-IGF1 Receptor/IGF1R Antibody Picoband&reg;IHC analysis of IGF1R using anti-IGF1R antibody (PA1746). <br>IGF1R was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1R Antibody (PA1746) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1746-igf1r-primary-antibodies-ihc-testing-1.jpgAnti-IGF1 Receptor/IGF1R Antibody Picoband&reg;IHC analysis of IGF1R using anti-IGF1R antibody (PA1746). <br>IGF1R was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IGF1R Antibody (PA1746) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd25-il-2sr-alpha-antibody-pa1747-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1747-il2ra-primary-antibodies-wb-testing-1.jpgAnti-IL2RA Antibody Picoband&reg; Western blot analysis of IL2RA using anti-IL2RA antibody (PA1747). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human CD25/IL2RA protein 10ng,<br> Lane 2: recombinant human CD25/IL2RA protein 5ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RA antigen affinity purified polyclonal antibody (Catalog # PA1747) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1747-il2ra-primary-antibodies-ihc-testing-2.jpgAnti-IL2RA Antibody Picoband&reg; IHC analysis of IL2RA using anti-IL2RA antibody (PA1747). <br> IL2RA was detected in a paraffin-embedded section of human lymph cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL2RA Antibody (PA1747) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1747-il2ra-primary-antibodies-wb-testing-3_1.jpgAnti-IL2RA Antibody Picoband&reg; Western blot analysis of IL2RA using anti-IL2RA antibody (PA1747). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk for 2 hour at RT. The membrane was incubated with anti-IL2RA antibody (PA1747) at 1:1000 for 16 hours at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a mouse anti-rabbit secondary antibody at a dilution of 1:10,000 for 2 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RA at approximately 45 kDa. The expected band size for IL2RA is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-3-antibody-pa1748-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1748-il3-primary-antibodies-wb-testing-1.jpgAnti-Interleukin-3 IL3 Antibody Picoband&reg;Western blot analysis of IL3 using anti-IL3 antibody (PA1748). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human IL3 protein 10 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL3 antigen affinity purified polyclonal antibody (PA1748) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL3 at approximately 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1748-il3-primary-antibodies-ihc-testing-1.jpgAnti-Interleukin-3 IL3 Antibody Picoband&reg;IHC analysis of IL3 using anti-IL3 antibody (PA1748). <br>IL3 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL3 Antibody (PA1748) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-4-antibody-pa1749-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1749-2-IHC-anti-il-4-antibody.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Anti-IL-4 antibody&#44; PA1749&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1749-1_1-WB-anti-il-4-antibody.jpgAnti-Interleukin-4 IL4 Antibody Picoband&reg;Anti-IL-4 antibody&#44; PA1749&#44; Western blotting<br>All lanes: Anti IL-4 (PA1749) at 0.5ug/ml<br> Lane 1: Recombinant Human IL-4 Protein 10ng<br> Lane 2: Recombinant Human IL-4 Protein 5ng<br> Lane 3: Recombinant Human IL-4 Protein 2.5ng<br> Predicted bind size: 14KD<br> Observed bind size: 14KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mag-antibody-pa1751-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1751-mag-primary-antibodies-ihc-testing-4.jpgAnti-Myelin-associated glycoprotein MAG Antibody Picoband&reg;IHC analysis of MAG using anti-MAG antibody (PA1751). <br>MAG was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAG Antibody (PA1751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1751-mag-primary-antibodies-wb-testing-1.jpgAnti-Myelin-associated glycoprotein MAG Antibody Picoband&reg; Western blot analysis of MAG using anti-MAG antibody (PA1751). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAG antigen affinity purified polyclonal antibody (Catalog # PA1751) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAG at approximately 100 kDa. The expected band size for MAG is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1751-mag-primary-antibodies-ihc-testing-2.jpgAnti-Myelin-associated glycoprotein MAG Antibody Picoband&reg; IHC analysis of MAG using anti-MAG antibody (PA1751). <br> MAG was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAG Antibody (PA1751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1751-mag-primary-antibodies-ihc-testing-3.jpgAnti-Myelin-associated glycoprotein MAG Antibody Picoband&reg; IHC analysis of MAG using anti-MAG antibody (PA1751). <br> MAG was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAG Antibody (PA1751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpc3-antibody-pa1753-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1753-1-WB-anti-trpc3-antibody.jpgAnti-TRPC3 Antibody Picoband&reg;Anti-TRPC3 antibody&#44; PA1753&#44; Western blotting<br>All lanes: Anti TRPC3 (PA1753) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: Rat Lung Tissue Lysate at 50ug<br>Lane 3: Rat Intestine Tissue Lysate at 50ug<br>Lane 4: Rat Ovary Tissue Lysate at 50ug<br>Lane 5: U87 Whole Cell Lysate at 40ug<br>Lane 6: A549 Whole Cell Lysate at 40ug<br>Lane 7: COLO320 Whole Cell Lysate at 40ug<br>Lane 8: SW620 Whole Cell Lysate at 40ug<br>Lane 9: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 97KD<br>Observed bind size: 120KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1753-2-IHC-anti-trpc3-antibody.jpgAnti-TRPC3 Antibody Picoband&reg;Anti-TRPC3 antibody&#44; PA1753&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1753-3.jpgAnti-TRPC3 Antibody Picoband&reg;Anti-TRPC3 antibody&#44; PA1753&#44; IHC(P)<br>IHC(P): Human Rectal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1753-4.jpgAnti-TRPC3 Antibody Picoband&reg; Western blot analysis of TRPC3 using anti-TRPC3 antibody (PA1753). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates&#44; <br> Lane 3: human Hela whole cell lysates&#44; <br> Lane 4: human 22RV1 whole cell lysates&#44; <br> Lane 5: human U-87MG whole cell lysates&#44; <br> Lane 6: mouse Neuro-2a whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC3 antigen affinity purified polyclonal antibody (Catalog # PA1753) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC3 at approximately 110KD. The expected band size for TRPC3 is at 93KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpc6-antibody-pa1754-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1754-1-WB-anti-trpc6-antibody.jpgAnti-TRPC6 Antibody Picoband&reg; Western blot analysis of TRPC6 using anti-TRPC6 antibody (PA1754). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Lung Tissue Lysate<br> Lane 2: 293T Cell Lysate<br> Lane 3: 293T Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody (Catalog # PA1754) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106-120KD. The expected band size for TRPC6 is at 106KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1754-2-IHC-anti-trpc6-antibody.jpgAnti-TRPC6 Antibody Picoband&reg; IHC analysis of TRPC6 using anti-TRPC6 antibody (PA1754).<br> TRPC6 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPC6 Antibody (PA1754) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1754-3-IHC-anti-trpc6-antibody.jpgAnti-TRPC6 Antibody Picoband&reg; IHC analysis of TRPC6 using anti-TRPC6 antibody (PA1754).<br> TRPC6 was detected in paraffin-embedded section of Rat Brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRPC6 Antibody (PA1754) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1754-4_1.jpgAnti-TRPC6 Antibody Picoband&reg; Western blot analysis of TRPC6 using anti-TRPC6 antibody (PA1754). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse lung Tissue Lysate<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody (Catalog # PA1754) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vip-receptor-1-antibody-pa1756-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1756-1-WB-anti-vip-receptor-1-antibody.jpgAnti-VIP Receptor 1/VIPR1 Antibody Picoband&reg;Anti-VIPR1 antibody&#44; PA1756&#44; Western blotting<br>All lanes: Anti VIPR1 (PA1756) at 0.5ug/ml<br>WB: Human Placenta Tissue Lysate at 50ug<br>Predicted bind size: 52KD<br>Observed bind size: 52KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-yb1-antibody-pa1758-boster.html2026-03-24T05:03:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1758-ybx1-primary-antibodies-wb-testing-1.jpgAnti-YB1/YBX1 Antibody Picoband&reg; Western blot analysis of YBX1 using anti-YBX1 antibody (PA1758). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates, <br> Lane 5: human T47D whole cell lysates, <br> Lane 6: human THP-1 whole cell lysates, <br> Lane 7: human MOLT4 whole cell lysates, <br> Lane 8: human HL-60 whole cell lysates, <br> Lane 9: rat testis tissue lysates, <br> Lane 10: mouse stomach tissue lysates, <br> Lane 11: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YBX1 antigen affinity purified polyclonal antibody (Catalog # PA1758) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YBX1 at approximately 50 kDa. The expected band size for YBX1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1758-ybx1-primary-antibodies-ihc-testing-2.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YBX1 using anti-YBX1 antibody (PA1758). <br> YBX1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YBX1 Antibody (PA1758) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1758-ybx1-primary-antibodies-ihc-testing-3.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YBX1 using anti-YBX1 antibody (PA1758). <br> YBX1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YBX1 Antibody (PA1758) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1758-ybx1-primary-antibodies-ihc-testing-4.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YBX1 using anti-YBX1 antibody (PA1758). <br> YBX1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YBX1 Antibody (PA1758) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1758-ybx1-primary-antibodies-ihc-testing-5.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YBX1 using anti-YBX1 antibody (PA1758). <br> YBX1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YBX1 Antibody (PA1758) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1758-ybx1-primary-antibodies-fcm-testing-6_1.jpgAnti-YB1/YBX1 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-YBX1 antibody (PA1758). <br>Overlay histogram showing HEL cells stained with PA1758 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YBX1 Antibody (PA1758, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cacybp-antibody-pa1759-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1759-cacybp-primary-antibodies-ip-testing-4.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; Immunoprecipitating CACYBP in U251 whole cell lysate.<br>Western blot analysis of CACYBP using anti-CACYBP antibody (PA1759). <br>Lane 1: U251 whole cell lysates (30ug),<br>Lane 2: Rabbit control IgG instead of anti-CACYBP antibody in U251 whole cell lysate,<br>Lane 3: anti-CACYBP antibody (2μg) + U251 whole cell lysate (500μg).<br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CACYBP antigen affinity purified polyclonal antibody (PA1759) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CACYBP at approximately 27 kDa. The expected band size for CACYBP is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1759-cacybp-primary-antibodies-wb-testing-1.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; Western blot analysis of CACYBP using anti-CACYBP antibody (PA1759). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human SW620 whole cell lysates, <br> Lane 4: human U251 whole cell lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: rat brain tissue lysates, <br> Lane 7: mouse liver tissue lysates, <br> Lane 8: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACYBP antigen affinity purified polyclonal antibody (Catalog # PA1759) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CACYBP at approximately 27 kDa. The expected band size for CACYBP is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1759-cacybp-primary-antibodies-if-testing-2.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; IF analysis of CACYBP using anti-CACYBP antibody (PA1759). <br> CACYBP was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CACYBP Antibody (PA1759) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1759-cacybp-primary-antibodies-fcm-testing-3.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-CACYBP antibody (PA1759). <br> Overlay histogram showing HL-60 cells stained with PA1759 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CACYBP Antibody (PA1759, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gro-alpha-antibody-pa1760-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1760-1-WB-anti-cxcl1-gro-alpha-antibody.jpgAnti-GRO alpha/CXCL1 Antibody Picoband&reg;Anti-GRO alpha antibody&#44; PA1760&#44; Western blotting<br>All lanes: Anti GRO alpha (PA1760) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: JURKAT Whole Cell Lysate at 40ug<br> Predicted bind size: 11KD<br> Observed bind size: 11KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dpyd-antibody-pa1761-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1761-dpyd-primary-antibodies-wb-testing-1.jpgAnti-DPYD Antibody Picoband&reg;Western blot analysis of DPYD using anti-DPYD antibody (PA1761). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human THP-1 whole cell lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DPYD antigen affinity purified polyclonal antibody (PA1761) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DPYD at approximately 111 kDa. The expected band size for DPYD is at 111 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1761-dpyd-primary-antibodies-fcm-testing-1.jpgAnti-DPYD Antibody Picoband&reg;Flow Cytometry analysis of HepG2 cells using anti-DPYD antibody (PA1761). <br> Overlay histogram showing HepG2 cells stained with PA1761 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DPYD Antibody (PA1761, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dusp3-antibody-pa1762-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1762-dusp3-primary-antibodies-wb-testing-1.jpgAnti-DUSP3 Antibody Picoband&reg; Western blot analysis of DUSP3 using anti-DUSP3 antibody (PA1762). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human MDA-MB-453 whole cell lysates,<br> Lane 3: human Caco-2 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat skeletal muscle tissue lysates,<br> Lane 6: rat heart tissue lysates,<br> Lane 7: mouse skeletal muscle tissue lysates,<br> Lane 8: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DUSP3 antigen affinity purified polyclonal antibody (Catalog # PA1762) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DUSP3 at approximately 20 kDa. The expected band size for DUSP3 is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dci-antibody-pa1763-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1763-eci1-primary-antibodies-wb-testing-1.jpgAnti-DCI/ECI1 Antibody Picoband&reg;Western blot analysis of ECI1 using anti-ECI1 antibody (PA1763). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human COLO320 whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECI1 antigen affinity purified polyclonal antibody (PA1763) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ECI1 at approximately 28 kDa. The expected band size for ECI1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1763-eci1-primary-antibodies-ihc-testing-1.jpgAnti-DCI/ECI1 Antibody Picoband&reg;IHC analysis of ECI1 using anti-ECI1 antibody (PA1763). <br>ECI1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECI1 Antibody (PA1763) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1763-eci1-primary-antibodies-ihc-testing-2.jpgAnti-DCI/ECI1 Antibody Picoband&reg;IHC analysis of ECI1 using anti-ECI1 antibody (PA1763). <br>ECI1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ECI1 Antibody (PA1763) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1763-eci1-primary-antibodies-fcm-testing-1.jpgAnti-DCI/ECI1 Antibody Picoband&reg;Flow Cytometry analysis of 293T cells using anti-ECI1 antibody (PA1763). <br>Overlay histogram showing 293T cells stained with PA1763 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ECI1 Antibody (PA1763, 1 μg/1x10⁶ cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fmo3-antibody-pa1764-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1764-1-WB-anti-fmo3-antibody.jpgAnti-FMO3 Antibody Picoband&reg;Anti-FMO3 antibody&#44; PA1764&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate <br>Lane 2: Rat Liver Tissue Lysate <br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gli3-antibody-pa1766-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1766-1-WB-anti-gli3-antibody.jpgAnti-Transcriptional activator GLI3 Gli3 Antibody Picoband&reg;Anti-Gli3 antibody&#44; PA1766&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate <br>Lane 2: A549 Cell Lysate <br>Lane 3: SW620 Cell Lysate <br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-6-antibody-pa1767-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1767-1-WB-anti-kallikrein-6-antibody.jpgAnti-Kallikrein 6/KLK6 Antibody Picoband&reg;Anti-Kallikrein 6 antibody&#44; PA1767&#44; Western blotting<br>All lanes: Anti Kallikrein 6 (PA1767) at 0.5ug/ml<br> Lane 1: MCF-7 Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: MM231 Whole Cell Lysate at 40ug<br> Lane 4: MM453 Whole Cell Lysate at 40ug<br> Lane 5: A549 Whole Cell Lysate at 40ug<br> Lane 6: SMMC Whole Cell Lysate at 40ug<br> Lane 7: COLO320 Whole Cell Lysate at 40ug<br> Lane 8: SW620 Whole Cell Lysate at 40ug<br> Lane 9: HT1080 Whole Cell Lysate at 40ug<br> Predicted bind size: 27KD<br> Observed bind size: 27KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1767-2-IHC-anti-kallikrein-6-antibody.jpgAnti-Kallikrein 6/KLK6 Antibody Picoband&reg;Anti-Kallikrein 6 antibody&#44; PA1767&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1767-3-IF-anti-kallikrein-6-antibody.jpgAnti-Kallikrein 6/KLK6 Antibody Picoband&reg;Anti-Kallikrein 6 antibody&#44; PA1767&#44; ICC<br>ICC: PC-3 Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm6-antibody-pa1769-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1769-1-WB-anti-mcm6-antibody.jpgAnti-MCM6 Antibody Picoband&reg;Anti-MCM6 antibody&#44; PA1769&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: MCF-7 Cell Lysate<br>Lane 5: JURKAT Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1769-2-IHC-anti-mcm6-antibody.jpgAnti-MCM6 Antibody Picoband&reg;Anti-MCM6 antibody&#44; PA1769&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1769-3-IHC-anti-mcm6-antibody.jpgAnti-MCM6 Antibody Picoband&reg;Anti-MCM6 antibody&#44; PA1769&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1769-4-IF-anti-mcm6-antibody.jpgAnti-MCM6 Antibody Picoband&reg;Anti-MCM6 antibody&#44; PA1769&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1769-5-IHC-anti-mcm6-antibody.jpgAnti-MCM6 Antibody Picoband&reg;Anti-MCM6 antibody&#44; PA1769&#44; IHC(F)<br>IHC(F): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1769-6.jpgAnti-MCM6 Antibody Picoband&reg; Western blot analysis of MCM6 using anti-MCM6 antibody (PA1769). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse small intestine tissue lysates&#44; <br> Lane 2: mouse spleen tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM6 antigen affinity purified polyclonal antibody (Catalog # PA1769) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM6 at approximately 93KD. The expected band size for MCM6 is at 93KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1769-7.jpgAnti-MCM6 Antibody Picoband&reg; IHC analysis of MCM6 using anti-MCM6 antibody (PA1769).<br> MCM6 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MCM6 Antibody (PA1769) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1769-mcm6-primary-antibodies-if-testing-8.jpgAnti-MCM6 Antibody Picoband&reg; IF analysis of MCM6 using anti-MCM6 antibody (PA1769). <br> MCM6 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MCM6 Antibody (PA1769) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1769-mcm6-primary-antibodies-fcm-testing-9.pngAnti-MCM6 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-MCM6 antibody (PA1769). <br>Overlay histogram showing SiHa cells stained with PA1769 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM6 Antibody (PA1769, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nrp2-antibody-pa1771-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1771-1-WB-anti-neuropilin-2-antibody.jpgAnti-Neuropilin-2 NRP2 Antibody Picoband&reg;Anti-NRP2 antibody&#44; PA1771&#44; Western blotting<br>All lanes: Anti NRP2 (PA1771) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 50ug<br> Lane 2: Rat Testis Tissue Lysate at 50ug<br> Predicted bind size: 105KD<br> Observed bind size: 105KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab5-antibody-pa1773-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1773-rab5a-primary-antibodies-wb-testing-1.jpgAnti-Rab5/RAB5A Antibody Picoband&reg;Western blot analysis of RAB5A using anti-RAB5A antibody (PA1773). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5A antigen affinity purified polyclonal antibody (PA1773) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB5A at approximately 24 kDa. The expected band size for RAB5A is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1773-2-IHC-anti-rab5-antibody.jpgAnti-Rab5/RAB5A Antibody Picoband&reg;Anti-Rab5 antibody&#44; PA1773&#44; IHC(P)<br>IHC(P):Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1773-rab5a-primary-antibodies-ihc-testing-3.jpgAnti-Rab5/RAB5A Antibody Picoband&reg;IHC analysis of RAB5A using anti-RAB5A antibody (PA1773). <br>RAB5A was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A Antibody (PA1773) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1773-rab5a-primary-antibodies-ihc-testing-4.jpgAnti-Rab5/RAB5A Antibody Picoband&reg;IHC analysis of RAB5A using anti-RAB5A antibody (PA1773). <br>RAB5A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A Antibody (PA1773) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1773-rab5a-primary-antibodies-ihc-testing-5.jpgAnti-Rab5/RAB5A Antibody Picoband&reg;IHC analysis of RAB5A using anti-RAB5A antibody (PA1773). <br>RAB5A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A Antibody (PA1773) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1773-rab5a-primary-antibodies-ihc-testing-6.jpgAnti-Rab5/RAB5A Antibody Picoband&reg;IHC analysis of RAB5A using anti-RAB5A antibody (PA1773). <br>RAB5A was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB5A Antibody (PA1773) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1773_rab5-pa1733-wb-anti-tlr7-antibody.jpgAnti-Rab5/RAB5A Antibody Picoband&reg; https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-snap23-antibody-pa1774-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1774-snap23-primary-antibodies-wb-testing-1.jpgAnti-SNAP23 Antibody Picoband&reg;Western blot analysis of SNAP23 using anti-SNAP23 antibody (PA1774). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human HEL whole cell lysates, <br> Lane 4: human CACO-2 whole cell lysates, <br> Lane 5: human THP-1 whole cell lysates, <br> Lane 6: human U2OS whole cell lysates, <br> Lane 7: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP23 antigen affinity purified polyclonal antibody (PA1774) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SNAP23 at approximately 23 kDa. The expected band size for SNAP23 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1774-2-IHC-anti-snap23-antibody.jpgAnti-SNAP23 Antibody Picoband&reg;Anti-SNAP23 antibody&#44; PA1774&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1774-4-IHC-anti-snap23-antibody.jpgAnti-SNAP23 Antibody Picoband&reg;Anti-SNAP23 antibody&#44; PA1774&#44; IHC(F)<br>IHC(F): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1774-3_1-IF-anti-snap23-antibody.jpgAnti-SNAP23 Antibody Picoband&reg;Anti-SNAP23 antibody&#44; PA1774&#44;ICC<br>ICC: K562 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1774-snap23-primary-antibodies-if-testing-5.jpgAnti-SNAP23 Antibody Picoband&reg; IF analysis of SNAP23 using anti-SNAP23 antibody (PA1774). <br> SNAP23 was detected in immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-SNAP23 Antibody (PA1774) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1774-snap23-primary-antibodies-ihc-testing-6.jpgAnti-SNAP23 Antibody Picoband&reg; IHC analysis of SNAP23 using anti-SNAP23 antibody (PA1774). <br> SNAP23 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SNAP23 Antibody (PA1774) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1774-snap23-primary-antibodies-fcm-testing-7.pngAnti-SNAP23 Antibody Picoband&reg; Flow Cytometry analysis of HEPG2 cells using anti-SNAP23 antibody (PA1774). <br>Overlay histogram showing HEPG2 cells stained with PA1774 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNAP23 Antibody (PA1774, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sod2-antibody-pa1776-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1776-sod2-primary-antibodies-wb-testing-1.jpgAnti-SOD2 Antibody Picoband&reg; Western blot analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody (PA1776). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: rat lung tissue lysates, <br> Lane 4: mouse liver tissue lysates, <br> Lane 5: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2/Mnsod antigen affinity purified polyclonal antibody (Catalog # PA1776) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2/Mnsod at approximately 24 kDa. The expected band size for SOD2/Mnsod is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1776-41598_2025_91311_fig7_html.pngAnti-SOD2 Antibody Picoband&reg;Kaempferol inhibits autophagy and attenuates oxidative stress in RA-FLS cells. ( A ) RA-FLS cells were treated with different concentrations of kaempferol, cellular reactive oxygen species were detected by DCFH-DA, and cell membrane potential was detected by JC-1, Scale bar is 100 μm. ( B ) RA-FLS cells were treated with different concentrations of kaempferol and protein expression of SOD2 was detected by Western blot. ( C ) In different concentrations of kaempferol-treated RA-FLS cells, Western blot detected protein expression of P62, LC3B. * P < 0.05; ** P < 0.01; *** P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-91311-6'>40221466</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1776-41598_2025_91311_fig8_html.pngAnti-SOD2 Antibody Picoband&reg;Kaempferol inhibits cellular autophagy through the MAPK8/NLRP3 pathway to attenuate the abnormal proliferation and inflammation of RA-FLS. ( A ) Different concentrations of kaempferol were treated with RA-FLS cells, and the protein expression of P-MAPK8 and MAPK8 were detected by Western blot. ( B ) In different concentrations of kaempferol-treated RA-FLS cells, Western blot detected protein expression of NLRP3, IL-1β. ( C ) Overexpression of MAPK8 and kaempferol treated RA-FLS cells, Western blot detected protein expression of NLRP3, IL-1β, PCNA, SOD2. ( D ) Overexpression of MAPK8 with kaempferol-treated RA-FLS cells and immunofluorescence detection of LC3B protein expression, Scale bar is 50 μm. ( E ) Overexpression of MAPK8 with kaempferol-treated RA-FLS cells and immunofluorescence detection of P62 protein expression, Scale bar is 50 μm. * P < 0.05; ** P < 0.01; *** P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-91311-6'>40221466</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1776-12967_2024_5228_fig3_html.pngAnti-SOD2 Antibody Picoband&reg;Resveratrol attenuates inflammation and oxidative stress in RA-ILD. A Immunohistochemical detection of TNF-α and IL-1β protein expression in lung tissue. B Western blotting was used to detect P-NFKB, NFKB, TNF-α, and IL-1β protein expression in lung tissue. C qRT-PCR for TNF-α, IL-1β RNA expression in lung tissue. D MDA detects lung tissue oxidation levels. E Western blotting to detect SOD1 and SOD2 protein expression in lung tissue. F Western blotting to detect P-NFKB, NFKB, IL-1β protein expression in MRC-5 cell model after resveratrol treatment. G Western blotting to detect HIF-1α, SOD2 protein expression in the MRC-5 cell model after resveratrol treatment. H DCFH-DA and JC-1 were used to detect the levels of ROS and membrane potential in the MRC-5 cell model after resveratrol treatment, respectively. * P < 0.05; ** P < 0.01; *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05228-1'>38745204</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1776-2-IHC-anti-sod2-mnsod-antibody.jpgAnti-SOD2 Antibody Picoband&reg; IHC analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody (PA1776).<br> SOD2/Mnsod was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD2/Mnsod Antibody (PA1776) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1776-3-IHC-anti-sod2-mnsod-antibody.jpgAnti-SOD2 Antibody Picoband&reg; IHC analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody (PA1776).<br> SOD2/Mnsod was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD2/Mnsod Antibody (PA1776) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1776-sod2-primary-antibodies-if-testing-4.jpgAnti-SOD2 Antibody Picoband&reg; IF analysis of SOD2 using anti-SOD2 antibody (PA1776). <br> SOD2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SOD2 Antibody (PA1776) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sptlc1-antibody-pa1777-boster.html2026-03-24T05:03:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1777-sptlc1-primary-antibodies-wb-testing-1.jpgAnti-Serine palmitoyltransferase 1 SPTLC1 Antibody Picoband&reg; Western blot analysis of SPTLC1 using anti-SPTLC1 antibody (PA1777). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SiHa whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPTLC1 antigen affinity purified polyclonal antibody (Catalog # PA1777) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPTLC1 at approximately 56 kDa. The expected band size for SPTLC1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1777-2-ihc-anti-sptlc1-antibody.jpgAnti-Serine palmitoyltransferase 1 SPTLC1 Antibody Picoband&reg; IHC analysis of SPTLC1 using antiSPTLC1 antibody (PA1777). <br> SPTLC1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SPTLC1 Antibody (PA1777) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cardiac-troponin-c-antibody-pa1779-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1779-1-WB-anti-cardiac-troponin-c-antibody.jpgAnti-cardiac Troponin C/TNNC1 Antibody Picoband&reg;Anti-cardiac Troponin C antibody&#44; PA1779&#44; Western blotting<br>All lanes: Anti cardiac Troponin C (PA1779) at 0.5ug/ml<br> WB: Rat Skeletal Muscle Tissue Lysate at 50ug<br> Predicted bind size: 18KD<br> Observed bind size: 18KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1779-2-IHC-anti-cardiac-troponin-c-antibody.jpgAnti-cardiac Troponin C/TNNC1 Antibody Picoband&reg;Anti-cardiac Troponin C antibody&#44; PA1779&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1779-tnnc1-primary-antibodies-ihc-testing-3.jpgAnti-cardiac Troponin C/TNNC1 Antibody Picoband&reg;IHC analysis of TNNC1 using anti-TNNC1 antibody (PA1779). <br>TNNC1 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNNC1 Antibody (PA1779) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vdac-porin-antibody-pa1780-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1780-vdac1-primary-antibodies-wb-testing-1.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; Western blot analysis of VDAC/Porin/VDAC1 using anti-VDAC/Porin/VDAC1 antibody (PA1780). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat NRK whole cell lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDAC/Porin/VDAC1 antigen affinity purified polyclonal antibody (Catalog # PA1780) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDAC/Porin/VDAC1 at approximately 34 kDa. The expected band size for VDAC/Porin/VDAC1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1780-vdac1-primary-antibodies-ihc-testing-2.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IHC analysis of VDAC/Porin/VDAC1 using anti-VDAC/Porin/VDAC1 antibody (PA1780). <br> VDAC/Porin/VDAC1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VDAC/Porin/VDAC1 Antibody (PA1780) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1780-vdac1-primary-antibodies-ihc-testing-3.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IHC analysis of VDAC/Porin/VDAC1 using anti-VDAC/Porin/VDAC1 antibody (PA1780). <br> VDAC/Porin/VDAC1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VDAC/Porin/VDAC1 Antibody (PA1780) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1780-vdac1-primary-antibodies-fcm-testing-7.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-VDAC/Porin/VDAC1 antibody (PA1780). <br>Overlay histogram showing Hela cells stained with PA1780 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDAC/Porin/VDAC1 Antibody (PA1780, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1780-vdac1-primary-antibodies-ihc-testing-4.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IHC analysis of VDAC/Porin/VDAC1 using anti-VDAC/Porin/VDAC1 antibody (PA1780). <br> VDAC/Porin/VDAC1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VDAC/Porin/VDAC1 Antibody (PA1780) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1780-vdac1-primary-antibodies-ihc-testing-5.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IHC analysis of VDAC/Porin/VDAC1 using anti-VDAC/Porin/VDAC1 antibody (PA1780). <br> VDAC/Porin/VDAC1 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VDAC/Porin/VDAC1 Antibody (PA1780) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1780-vdac1-primary-antibodies-if-testing-6.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IF analysis of VDAC/Porin/VDAC1 using anti-VDAC/Porin/VDAC1 antibody (PA1780). <br> VDAC/Porin/VDAC1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-VDAC/Porin/VDAC1 Antibody (PA1780) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vinculin-antibody-pa1781-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1781-1-WB-anti-vinculin-antibody.jpgAnti-Vinculin/VCL Antibody Picoband&reg;Anti-Vinculin antibody&#44; PA1781&#44; Western blotting<br>Lane 1: Rat Heart Tissue Lysate <br>Lane 2: Rat Brain Tissue Lysate <br>Lane 3: Rat Liver Tissue Lysate <br>Lane 4: U87 Cell Lysate <br>Lane 5: SMMC Cell Lysate <br>Lane 6: HEPA Cell Lysate <br>Lane 7: HELA Cell Lysate<br> Lane 8: HT1080 Cell Lysate https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1781-2-IHC-anti-vinculin-antibody.jpgAnti-Vinculin/VCL Antibody Picoband&reg;Anti-Vinculin antibody&#44; PA1781&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1781-3-IHC-anti-vinculin-antibody.jpgAnti-Vinculin/VCL Antibody Picoband&reg;Anti-Vinculin antibody&#44; PA1781&#44; IHC(P)<br>IHC(P): Rat Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ahr-antibody-pa1782-1-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-wb-testing-1.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; Western blot analysis of AHR using anti-AHR antibody (PA1782-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HELA whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # PA1782-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 100-110KD. The expected band size for AHR is at 100-110KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-thnov10p12011g001.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg;High inflammation status and AhR dysregulation in stone patient kidneys while reducing renal inflammation and injury found in AhR-activated CaOx nephrocalcinosis mice. (A) PET-CT imaging studies assessing renal inflammatory responsiveness. Axial CT, axial PET, axial fused PET-CT and coronal PET maximum intensity projection (MIP) images suggesting enhanced renal uptake of 18 F-FDG. Arrows are used to mark focal 18 F-FDG accumulation in the form of a ring surrounding the stone. (B) crystal deposition in Randall's plaques (n = 10) was analysed via polarized light optical microscopy (100×; scale bar: 20 µm) and IHC staining for AhR in Randall's plaques (200×; scale bar: 20 µm). (C) Deposition of renal CaOx crystal in the corticomedullary junction of mice (n = 6) treated with increasing concentrations of FICZ was analysed via polarized light optical microscopy (20×, scale bar: 500 µm). Crystal deposition within corticomedullary junction regions was further confirmed by Pizzolato staining (200×; scale bar: 20 µm). Kidney injury and necrosis were evaluated by PAS staining (200×; scale bar: 20 µm) and TUNEL staining (200×; scale bar: 50 µm) in kidney tissues, respectively.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-thnov10p12011g002.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg;AhR significantly suppressed IRF1 and HIF-1α expression in a murine CaOx nephrocalcinosis model. (A) RNA-seq heatmap showing significantly altered mRNAs in SGA-treated BMDMs. (B) Volcano plots showing mRNA transcripts that were differentially expressed between LPS-treated and SGA-treated BMDMs. Significantly downregulated and upregulated mRNAs are shown in green and red, respectively, whereas genes that were not significantly changed are shown in black. (C) IHC staining for AhR, IRF1, and HIF-1α in the kidneys of FICZ-treated mice with CaOx nephrocalcinosis (200×; scale bar: 20 µm). (D) qRT-PCR was used to assess AhR, IRF1, and HIF-1α expression in kidney samples from FICZ-treated mice (n = 6) with CaOx nephrocalcinosis compared to kidney samples from model mice. (E, F) Pearson's correlation coefficient analysis (n = 30) of the expression levels of AhR and IRF1 (E) or HIF-1α (F). Each dot represents an individual animal. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (D).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-thnov10p12011g003.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg;AhR suppressed IRF1 and HIF-1α to attenuate CaOx crystal-stimulated M1 macrophage polarization in vitro . (A) BMDMs and COM-treated TECs co-culture model. (B, C) Western blotting analysis was used to detect AhR, HIF-1α, IRF1, NF-κB p65, iNOS, and Arg-1 expression after FICZ treatment and the upregulation or downregulation of AhR in BMDMs. β-actin served as a normalization control. (D, E) iNOS (M1 macrophage marker, green) and Arg-1 (M2 macrophage marker, red) distribution in BMDMs were detected by immunofluorescence (200×; scale bar: 20 µm). (F, G) qRT-PCR analysis of iNOS, IL-6, CIITA, Arg-1, Chi3l3 and Fizz1 expression to further determine polarization state of BMDM. The data are shown as the means ± SD of triplicate experiments. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (F, G).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-thnov10p12011g005.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg;AhR activation in vitro decrease M1 macrophage polarization to inhibit kidney inflammation and injury through the AhR-miR-142a-IRF1/HIF-1α axis in vitro . (A) Western blotting analysis enabled the detection of AhR, HIF-1α, IRF1, NF-κB p65, iNOS, and Arg-1 expression in BMDMs. β-actin was detected as an internal control. (B) iNOS (M1 macrophage marker, green) and Arg-1 (M2 macrophage marker, red) distributions in BMDMs were detected by immunofluorescence (200×; scale bar: 20 µm). (C) Schematic diagram of BMDMs phagocytic capacity testing. (D) Fluorescence microscopy was performed to analyse the phagocytic ability of BMDMs (200×; scale bar: 20 µm). (E) qRT-PCR analysis of iNOS, IL-6, CIITA, Arg-1, Chi3l3 and Fizz1 expression to further determine polarization state of BMDM. (F) ELISA was used to quantify cytokine levels in the co-culture media. The data are shown as the means ± SD of triplicate experiments. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (E, F).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-thnov10p12011g004.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg;AhR transcriptionally activates miR-142a to inhibit IRF1 and HIF-1α expression. (A) The top 30 miRNAs in BMDMs that are regulated by LPS are arranged in a miRNA array heatmap. In addition, miRNAs predicted to be under the transcriptional control of AhR (according to analysis with the JASPAR database) are noted. (B) Venn diagram analyses were performed to identify miRNAs that can both target IRF1 and HIF-1α and that are under the transcriptional control of AhR. (C) Renal expression of mmu-miR-142a-3p in mice (n = 6) with CaOx nephrocalcinosis following treatment with an AhR neutralizing antibody or FICZ treatment was assessed via FISH (200×; scale bar: 20 µm). (D) qRT-PCR was performed to measure mmu-miR-142a-3p expression in BMDMs using U6 RNA as a normalization control. (E, F) ChIP assays and ChIP qPCR analysis showed that AhR bound to the miR-142a promoter in BMDMs treated with the AhR overexpression plasmid. (G) A schematic model showed that AhR directly binds to the miR-142a promoter and activates its transcription. (H, I) WT and mutated miR-142a targeting sequences in the IRF1 and HIF-1α 3'-UTR regions that were used to construct luciferase reporters, with reporters bearing these IRF1 (J) or HIF-1α (L) 3'-UTR sequences co-transfected along with miR-142a mimic (100 nM). IRF1 (K) and HIF-1α (M) mRNA levels were detected via qRT-PCR in BMDMs following miR-142a mimic or inhibitor transfection. Western blotting (N, O) analysis enabled the detection of IRF1 and HIF-1α expression while also assessing the levels of iNOS and Arg-1 to monitor the polarization state of BMDMs following miR-142a mimic or inhibitor transfection. β-actin was employed as a normalization control. The data are shown as the means ± SD of triplicate experiments. *P < 0.05; **P < 0.01, as assessed via Student's t test (D, F) or one-way ANOVA (J-M, O).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-thnov10p12011g006.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg;AhR activation suppressed the deposition of CaOx crystal and CaOx nephrocalcinosis-mediated kidney inflammation and injury through the AhR-miR-142a-IRF1/HIF-1α axis in vivo . (A) Experimental overview. (B) The deposition of renal CaOx crystal in FICZ- and/or antagomiR-142a-treated mice was assessed via polarized light optical microscopy (20×; scale bar: 500 µm). Pizzolato staining was employed as a means of detecting these CaOx crystal in corticomedullary tissue, while PAS was utilized to evaluate injury to TECs (200×; scale bar: 20 µm), and TUNEL staining was employed to assess renal TECs death (200×; scale bar: 50 µm). (C) PET-CT scanning was employed as a means of assessing renal inflammation state in CaOx nephrocalcinosis mice. (D) IHC was used to analyse AhR, IRF1, and HIF-1α expression, and FISH was used to detect miR-142a expression in renal tissue (200×; scale bar: 20 µm). (E) iNOS (M1 macrophage marker, red) and Arg-1 (M2 macrophage marker, green) distributions in renal tissues were detected by immunofluorescence (200×; scale bar: 50 µm). (F) On days 3 and 10, the serum pro-inflammatory IL-1β, TNF-α, and IL-6 levels and the anti-inflammatory IL-10 levels were measured by ELISA. n = 6 per group. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (F).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-ihc-testing-2.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IHC analysis of AHR using anti-AHR antibody (PA1782-1). <br> AHR was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-ihc-testing-3.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IHC analysis of AHR using anti-AHR antibody (PA1782-1). <br> AHR was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-ihc-testing-4.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IHC analysis of AHR using anti-AHR antibody (PA1782-1). <br> AHR was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-ihc-testing-5.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IHC analysis of AHR using anti-AHR antibody (PA1782-1). <br> AHR was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-ihc-testing-6.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IHC analysis of AHR using anti-AHR antibody (PA1782-1). <br> AHR was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-ihc-testing-7.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IHC analysis of AHR using anti-AHR antibody (PA1782-1). <br> AHR was detected in paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-ihc-testing-8.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IHC analysis of AHR using anti-AHR antibody (PA1782-1). <br> AHR was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-if-testing-9.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; IF analysis of AHR using anti-AHR antibody PA1782-1). <br> AHR was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-AHR Antibody (PA1782-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1782-1-ahr-primary-antibodies-fcm-testing-10.pngAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-AHR antibody (PA1782-1). <br>Overlay histogram showing A431 cells stained with PA1782-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AHR Antibody (PA1782-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-liver-arginase-antibody-pa1783-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1783-arg1-primary-antibodies-wb-testing-1.jpgAnti-liver Arginase/ARG1 Antibody Picoband&reg; Western blot analysis of ARG1 using anti-ARG1 antibody (PA1783). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 antigen affinity purified polyclonal antibody (Catalog # PA1783) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG1 at approximately 38 kDa. The expected band size for ARG1 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atm-antibody-pa1784-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1784-1-WB-anti-atm-antibody.jpgAnti-Serine-protein kinase ATM ATM Antibody Picoband&reg;Anti-ATM antibody&#44; PA1784&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate <br>Lane 2: U87 Cell Lysate <br>Lane 3: MCF-7 Cell Lysate <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1784-2-WB-anti-atm-antibody.jpgAnti-Serine-protein kinase ATM ATM Antibody Picoband&reg;Anti-ATM antibody&#44; PA1784&#44; Western blotting<br>Lane 1: HELA Cell Lysate <br>Lane 2: SMMC Cell Lysate <br>Lane 3: U87 Cell Lysate <br>Lane 4: A549 Cell Lysate <br>Lane 5: MCF-7 Cell Lysate <br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aurora-a-antibody-pa1785-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1785-1_1-WB-anti-aurora-a-antibody.jpgAnti-Aurora A/AURKA Antibody Picoband&reg;Anti-AURKA antibody&#44; PA1785&#44; Western blotting<br>All lanes: Anti AURKA (PA1785) at 0.5ug/ml<br>WB: Mouse Ovary Tissue Lysate at 50ug<br>Predicted bind size: 46KD<br>Observed bind size: 46KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cish-antibody-pa1786-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1786-cish-primary-antibodies-wb-testing-1.jpgAnti-CISH Antibody Picoband&reg; Western blot analysis of CISH using anti-CISH antibody (PA1786). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human COLO320 whole cell lysates,<br> Lane 4: human CACO-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CISH antigen affinity purified polyclonal antibody (Catalog # PA1786) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CISH at approximately 37 kDa. The expected band size for CISH is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1786-2-IHC-anti-cish-cis-1-antibody.jpgAnti-CISH Antibody Picoband&reg;Anti-CISH antibody&#44; PA1786&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1786-3-IHC-anti-cish-cis-1-antibody.jpgAnti-CISH Antibody Picoband&reg;Anti-CISH antibody&#44; PA1786&#44; IHC(P)<br>IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1786-jitc-2022-006002f06.jpgAnti-CISH Antibody Picoband&reg;TIPE2/CISH single or double deletion for adoptive NK cell therapy. (A) Scheme ofthe generation and expansion of WT or TIPE2/CISH -single or TIPE2/CISH double-deleted human peripheral blood-derived NK cells for adoptive transfer. NK cells were enriched from PBMCs of healthy donors and electroporated with Cas9 protein and either one or both TIPE2/CISH- specific gRNAs. NK cells were expanded in a feeder-free manner and stimulated with 20 ng/mL IL-12, 50 ng/mL IL-15, and 10 ng/mL IL-18 for 2 days before transfer into NDG hIL-15 mice challenged with K562/HCT15 tumor cells 3 days prior. (B) Schema of CRISPR-Cas9-mediated CISH deletion using two guide RNAs (gRNAs) targeting exon 4 of the CISH gene in the direct and complementary strands. (C–E) K562 tumor growth and endpoint tumor mass are shown for (A). Groups of six mice per experiment were used. (C, E) Data are presented as the mean±SEM. *p<0.05, **p<0.005, ***p<0.001. (Two-way ANOVA (C), one-way ANOVA (E)). ANOVA, analysis of variance; PBMCs, peripheral blood mononuclear cells.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9896240/&orig_host=www.ncbi.nlm.nih.gov'>36725083</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1786-cish-primary-antibodies-if-testing-4.jpgAnti-CISH Antibody Picoband&reg; IF analysis of CISH using anti-CISH antibody (PA1786). <br> CISH was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CISH Antibody (PA1786) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1786-cish-primary-antibodies-if-testing-5.jpgAnti-CISH Antibody Picoband&reg; IF analysis of CISH using anti-CISH antibody (PA1786). <br> CISH was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CISH Antibody (PA1786) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hexa-antibody-pa1787-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1787-1-WB-anti-hexa-hexosaminidase-a-antibody.jpgAnti-HEXA Antibody Picoband&reg;Anti-HEXA antibody&#44; PA1787&#44; Western blotting<br>All lanes: Anti HEXA (PA1787) at 0.5ug/ml<br> Lane 1: Rat Liver Tissue Lysate at 50ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 61KD<br> Observed bind size: 61KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1787-2-IHC-anti-hexa-hexosaminidase-a-antibody.jpgAnti-HEXA Antibody Picoband&reg;Anti-HEXA antibody&#44; PA1787&#44; IHC(P)<br>IHC(P):Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hexa-antibody-pa1787-2-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1787-2-hexa-primary-antibodies-wb-testing-1_1.jpgAnti-HEXA Antibody Picoband&reg; Western blot analysis of HEXA using anti-HEXA antibody (PA1787-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 4: human THP-1 whole cell lysates, <br> Lane 5: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXA antigen affinity purified polyclonal antibody (Catalog # PA1787-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEXA at approximately 57 kDa. The expected band size for HEXA is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hla-dmb-antibody-pa1788-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1788-1-WB-anti-hla-dmb-antibody.jpgAnti-HLA DMB/HLA-DMB Antibody Picoband&reg;Anti-HLA DMB antibody&#44; PA1788&#44; Western blotting<br>All lanes: Anti HLA DMB (PA1788) at 0.5ug/ml<br> Lane 1: JURKAT Whole Cell Lysate at 40ug<br> Lane 2: JURKAT Whole Cell Lysate at 40ug<br> Lane 3: RAJI Whole Cell Lysate at 40ug<br> Lane 4: HUT Whole Cell Lysate at 40ug<br> Predicted bind size: 29KD<br> Observed bind size: 29KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1788-2-IHC-anti-hla-dmb-antibody.jpgAnti-HLA DMB/HLA-DMB Antibody Picoband&reg;Anti-HLA DMB antibody&#44; PA1788&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1788-41419_2015_article_bfcddis2015225_fig5_html.jpgAnti-HLA DMB/HLA-DMB Antibody Picoband&reg;miR-411-5p activate p38MAPK pathway by targeting SPRY4-induced terminal differentiation of RMS. ( a ) Western blot analysis of p38MAPK activation (P-p38MAPK: p-38) in RMS cells treated with HA-tagged MKK6EE protein-expressing pcDNA3, SPRY4 siRNA, or both. ( b ) Western blot analysis of p38MAPK activation (P-p38MAPK:p-38) in RMS cells treated with miR-411-5p-M, miR-411-5p-I, and MKK6EE protein-expressing pcDNA3 plus miR-411-5p-M. ( c ) Terminal differentiation of RD cells after treatment with SPRY4 siRNA or miR-411-5p-M for 6 and 48 h. Cells stained with apoptosis marker (caspase-3); cell cycle was analyzed using propidium iodide (PI) and showed changed morphology (MHC and myosin) after activation of p38MAPK caused by SPRY4 siRNA or miR-411-5p-M. Scale bars: panel (Caspase-3)=100 μ m, panel (MHC and Myosin)=20 μ m. ( d ) Quantitation of terminal differentiation of RD cells after treatment with SPRY4 siRNA or miR-411-5p-M for 6 and 48 h. ( e ) Graphical representation of cell cycle phase proportions in RD cells after treatment with miR-411-5p-M for 6 and 48 h. ( f ) Proliferation of RD cells was evaluated by [ 3 H] thymidine incorporation assay after treatment with SPRY4 siRNA or miR-411-5p-M for 4 days. The amount of [ 3 H] thymidine incorporated was measured with a liquid scintillation counter. The OD at 557 nm was determined using a microplate reader. ( g ) Western blot analysis of cleaved caspase-3 in RMS cells treated with SPRY4 siRNA or miR-411-5p-M for 24 and 72 h. Each assay ( a – g ) was conducted at least three times independently. Error bars indicate S.D. * P <0.05; ** P <0.01; *** P <0.005 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis2015225'>26291313</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grp75-antibody-pa1789-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1789-grp75-primary-antibodies-wb-testing-1.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; Western blot analysis of Grp75 using anti-Grp75 antibody (PA1789). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: rat testis tissue lysates, <br> Lane 6: mouse testis tissue lysates, <br> Lane 7: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Grp75 antigen affinity purified polyclonal antibody (Catalog # PA1789) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Grp75 at approximately 75 kDa. The expected band size for Grp75 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1789-2-IHC-anti-grp75-antibody.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; IHC analysis of Grp75 using anti-Grp75 antibody (PA1789). <br> Grp75 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Grp75 Antibody (PA1789) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1789-3-IHC-anti-grp75-antibody.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; IHC analysis of Grp75 using anti-Grp75 antibody (PA1789). <br> Grp75 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Grp75 Antibody (PA1789) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1789-4-IF-anti-grp75-antibody.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; ICC analysis of Grp75 using anti-Grp75 antibody (PA1789). <br> Grp75 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-Grp75 Antibody (PA1789) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1789-5.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; IHC analysis of Grp75 using anti-Grp75 antibody (PA1789). <br> Grp75 was detected in frozen section of rat liver tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Grp75 Antibody (PA1789) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cpn10-antibody-pa1790-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1790-1-IF-anti-cpn10-antibody.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IHC analysis of Cpn10 using anti-Cpn10 antibody (PA1790).<br> Cpn10 was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1790-hspe-primary-antibodies-if-testing-10.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IF analysis of Cpn10 using anti-Cpn10 antibody (PA1790). <br> Cpn10 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1790-hspe-primary-antibodies-wb-testing-2.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; Western blot analysis of Cpn10 using anti-Cpn10 antibody (PA1790). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: rat heart tissue lysates, <br> Lane 4: mouse heart tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cpn10 antigen affinity purified polyclonal antibody (Catalog # PA1790) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cpn10 at approximately 11KD. The expected band size for Cpn10 is at 11KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1790-3-IHC-anti-cpn10-antibody.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IHC analysis of Cpn10 using anti-Cpn10 antibody (PA1790).<br> Cpn10 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1790-4-IHC-anti-cpn10-antibody.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IHC analysis of Cpn10 using anti-Cpn10 antibody (PA1790).<br> Cpn10 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1790-5-IHC-anti-cpn10-antibody.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IHC analysis of Cpn10 using anti-Cpn10 antibody (PA1790).<br> Cpn10 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1790-6.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IF analysis of Cpn10 using anti-Cpn10 antibody (PA1790).<br> Cpn10 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1790-hspe-primary-antibodies-ihc-testing-7.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IHC analysis of Cpn10 using anti-Cpn10 antibody (PA1790). <br> Cpn10 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1790-hspe-primary-antibodies-if-testing-8.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IF analysis of Cpn10 using anti-Cpn10 antibody (PA1790). <br> Cpn10 was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594 Conjugated Avidin (BA1142). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1790-hspe-primary-antibodies-if-testing-9.jpgAnti-Cpn10/HSPE1 Antibody Picoband&reg; IF analysis of Cpn10 using anti-Cpn10 antibody (PA1790). <br> Cpn10 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Cpn10 Antibody (PA1790) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1790-hspe-primary-antibodies-fcm-testing-11.pngAnti-Cpn10/HSPE1 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Cpn10 antibody (PA1790). <br>Overlay histogram showing HepG2 cells stained with PA1790 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cpn10 Antibody (PA1790, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lasp1-antibody-pa1791-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1791-1-WB-anti-lasp1-antibody.jpgAnti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband&reg;Anti-LASP1 antibody&#44; PA1791&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate <br>Lane 2: Rat Spleen Tissue Lysate <br>Lane 3: Rat Intestine Tissue Lysate <br>Lane 4: JURKAT Cell Lysate <br>Lane 5: MCF-7 Cell Lysate <br>Lane 6: A431 Cell Lysate <br>Lane 7: HELA Cell Lysate <br> Lane 8: 293T Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1791-2-IHC-anti-lasp1-antibody.jpgAnti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband&reg;Anti-LASP1 antibody&#44; PA1791&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1791-3-IF-anti-lasp1-antibody.jpgAnti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband&reg;Anti-LASP1 antibody&#44; PA1791&#44; ICC<br>ICC: A549 Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lasp1-antibody-pa1791-1-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1791-1-1-WB-anti-lasp1-antibody.jpgAnti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband&reg;Anti-LASP1 antibody&#44; PA1791-1&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1791-1-2-IHC-anti-lasp1-antibody.jpgAnti-LIM and SH3 domain protein 1 LASP1 Antibody Picoband&reg;Anti-LASP1 antibody&#44; PA1791-1&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm7-antibody-pa1792-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1792-mcm7-primary-antibodies-fcm-testing-6.jpgAnti-MCM7 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-MCM7 antibody (PA1792). <br>Overlay histogram showing HL-60 cells stained with PA1792 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM7 Antibody (PA1792, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1792-mcm7-primary-antibodies-wb-testing-1.jpgAnti-MCM7 Antibody Picoband&reg; Western blot analysis of MCM7 using anti-MCM7 antibody (PA1792). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # PA1792) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 80 kDa. The expected band size for MCM7 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1792-mcm7-primary-antibodies-ihc-testing-2.jpgAnti-MCM7 Antibody Picoband&reg; IHC analysis of MCM7 using anti-MCM7 antibody (PA1792). <br> MCM7 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCM7 Antibody (PA1792) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1792-mcm7-primary-antibodies-ihc-testing-3.jpgAnti-MCM7 Antibody Picoband&reg; IHC analysis of MCM7 using anti-MCM7 antibody (PA1792). <br> MCM7 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCM7 Antibody (PA1792) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1792-mcm7-primary-antibodies-ihc-testing-4.jpgAnti-MCM7 Antibody Picoband&reg; IHC analysis of MCM7 using anti-MCM7 antibody (PA1792). <br> MCM7 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCM7 Antibody (PA1792) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1792-mcm7-primary-antibodies-if-testing-5.jpgAnti-MCM7 Antibody Picoband&reg; IF analysis of MCM7 using anti-MCM7 antibody (PA1792) and anti-Tubulin Alpha antibody (M03989-3).<br> MCM7 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MCM7 Antibody (PA1792) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apoptosis-repressor-with-card-antibody-pa1793-boster.html2026-03-24T05:03:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02831-5-nol3-primary-antibodies-wb-testing-1.jpgAnti-Apoptosis repressor with CARD/NOL3 Antibody Picoband&reg; Western blot analysis of NOL3 using anti-NOL3 antibody (A02831-5). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human SiHa whole cell lysates, <br> Lane 5: rat heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOL3 antigen affinity purified polyclonal antibody (A02831-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOL3 at approximately 27 kDa. The expected band size for NOL3 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prnp-antibody-pa1794-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1794-1_1.jpgAnti-Prion protein PrP/PRNP Antibody Picoband&reg;Anti-PRNP antibody&#44; PA1794&#44; Western blotting<br>Lane 1: U87 Cell Lysate <br>Lane 2: U87 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prnp-antibody-pa1795-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1795-1-WB-anti-prnp-prp-antibody.jpgAnti-Prion protein PrP/PRNP Antibody Picoband&reg;Anti-PRNP antibody&#44; PA1795&#44; Western blotting<br>All lanes: Anti PRNP (PA1795) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 50ug<br> Lane 2: Rat Brain Tissue Lysate at 50ug<br> Predicted bind size: 28KD<br> Observed bind size: 28KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1795-2-IHC-anti-prnp-prp-antibody.jpgAnti-Prion protein PrP/PRNP Antibody Picoband&reg;Anti-PRNP antibody&#44; PA1795&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ssr3-antibody-pa1796-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-wb-testing-1.jpgAnti-SSR3 Antibody Picoband&reg; Western blot analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: monkey COS-7 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSR3 antigen affinity purified polyclonal antibody (Catalog # PA1796) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSR3 at approximately 21 kDa. The expected band size for SSR3 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-ihc-testing-2.jpgAnti-SSR3 Antibody Picoband&reg; IHC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-ihc-testing-3.jpgAnti-SSR3 Antibody Picoband&reg; IHC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-ihc-testing-4.jpgAnti-SSR3 Antibody Picoband&reg; IHC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-ihc-testing-5.jpgAnti-SSR3 Antibody Picoband&reg; IHC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of mouse cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-ihc-testing-6.jpgAnti-SSR3 Antibody Picoband&reg; IHC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of mouse hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-ihc-testing-7.jpgAnti-SSR3 Antibody Picoband&reg; IHC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of rat cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-ihc-testing-8.jpgAnti-SSR3 Antibody Picoband&reg; IHC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of rat hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-9.jpgAnti-SSR3 Antibody Picoband&reg; ICC analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-if-testing-10.jpgAnti-SSR3 Antibody Picoband&reg; IF analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-if-testing-11.jpgAnti-SSR3 Antibody Picoband&reg; IF analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1796-ssr3-primary-antibodies-if-testing-12.jpgAnti-SSR3 Antibody Picoband&reg; IF analysis of SSR3 using anti-SSR3 antibody (PA1796). <br> SSR3 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-14-3-3-sigma-antibody-pa1797-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1797-sfn-primary-antibodies-wb-testing-1.jpgAnti-14-3-3 sigma/SFN Antibody Picoband&reg; Western blot analysis of SFN using anti-SFN antibody (PA1797). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: mouse brain tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFN antigen affinity purified polyclonal antibody (Catalog # PA1797) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFN at approximately 28KD. The expected band size for SFN is at 28KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1797-sfn-primary-antibodies-ihc-testing-2.jpgAnti-14-3-3 sigma/SFN Antibody Picoband&reg; IHC analysis of SFN using anti-SFN antibody (PA1797). <br> SFN was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFN Antibody (PA1797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1797-sfn-primary-antibodies-ihc-testing-3.jpgAnti-14-3-3 sigma/SFN Antibody Picoband&reg; IHC analysis of SFN using anti-SFN antibody (PA1797). <br> SFN was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFN Antibody (PA1797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1797-sfn-primary-antibodies-ihc-testing-4.jpgAnti-14-3-3 sigma/SFN Antibody Picoband&reg; IHC analysis of SFN using anti-SFN antibody (PA1797). <br> SFN was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFN Antibody (PA1797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1797-sfn-primary-antibodies-if-testing-5.jpgAnti-14-3-3 sigma/SFN Antibody Picoband&reg; IF analysis of SFN using anti-SFN antibody (PA1797). <br> SFN was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SFN Antibody (PA1797) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1797-sfn-primary-antibodies-if-testing-6.jpgAnti-14-3-3 sigma/SFN Antibody Picoband&reg; IF analysis of SFN using anti-SFN antibody (PA1797). <br> SFN was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SFN Antibody (PA1797) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1797-sfn-primary-antibodies-fcm-testing-7.jpgAnti-14-3-3 sigma/SFN Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-SFN antibody (PA1797).<br> Overlay histogram showing PA1797 cells stained with PA1797 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFN Antibody (PA1797,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tfpi-antibody-pa1798-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1798-1-WB-anti-tfpi-antibody.jpgAnti-TFPI Antibody Picoband&reg;Anti-TFPI antibody&#44; PA1798&#44; Western blotting<br>All lanes: Anti TFPI (PA1798) at 0.5ug/ml<br>WB: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 26KD<br>Observed bind size: 55KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1798-2-IHC-anti-tfpi-antibody.jpgAnti-TFPI Antibody Picoband&reg;Anti-TFPI antibody&#44; PA1798&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1798-3-IHC-anti-tfpi-antibody.jpgAnti-TFPI Antibody Picoband&reg;Anti-TFPI antibody&#44; PA1798&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cbl-antibody-pa1800-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1800-cbl-primary-antibodies-wb-testing-1.jpgAnti-CBL Antibody Picoband&reg; Western blot analysis of CBL using anti-CBL antibody (PA1800). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: rat RH35 whole cell lysates, <br> Lane 3: mouse ANA-1 whole cell lysates, <br> Lane 4: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBL antigen affinity purified polyclonal antibody (Catalog # PA1800) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBL at approximately 120 kDa. The expected band size for CBL is at 120 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hspb2-antibody-pa1802-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1802-hspb2-primary-antibodies-wb-testing-1.jpgAnti-Heat shock protein beta-2 HSPB2 Antibody Picoband&reg; Western blot analysis of HSPB2 using anti-HSPB2 antibody (PA1802). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U20S whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates,<br> Lane 3: rat H9C2 whole cell lysates,<br> Lane 4: rat heart tissue lysates,<br> Lane 5: mouse C2C12 whole cell lysates,<br> Lane 6: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB2 antigen affinity purified polyclonal antibody (Catalog # PA1802) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB2 at approximately 23 kDa. The expected band size for HSPB2 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1802-hspb2-primary-antibodies-ihc-testing-3.jpgAnti-Heat shock protein beta-2 HSPB2 Antibody Picoband&reg;IHC analysis of HSPB2 using anti-HSPB2 antibody (PA1802). <br>HSPB2 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPB2 Antibody (PA1802) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1802-3-IHC-anti-hspb2-antibody.jpgAnti-Heat shock protein beta-2 HSPB2 Antibody Picoband&reg;Anti-HSPB2 antibody&#44; PA1802&#44; IHC(F)<br>IHC(F): Rat Cardiac Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1802-1-IF-anti-hspb2-antibody.jpgAnti-Heat shock protein beta-2 HSPB2 Antibody Picoband&reg;Anti-HSPB2 antibody&#44; PA1802&#44; ICC<br>ICC: HELA Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rel-b-antibody-pa1803-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1803-1-WB-anti-rel-b-antibody.jpgAnti-Rel B/RELB Antibody Picoband&reg;Anti-Rel B antibody&#44; PA1803&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: NIH3T3 Cell Lysate<br>Lane 4: RAJI Cell Lysate<br>Lane 5: HEPA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-paxillin-antibody-pa1804-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1804-pxn-primary-antibody-wb-testing-1.jpgAnti-Paxillin/PXN Antibody Picoband&reg; Western blot analysis of PXN using anti-PXN antibody (PA1804). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human HEL whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PXN antigen affinity purified polyclonal antibody (Catalog # PA1804) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PXN at approximately 65 kDa. The expected band size for PXN is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1804-2-IHC-anti-paxillin-antibody.jpgAnti-Paxillin/PXN Antibody Picoband&reg; IHC analysis of PXN using anti-PXN antibody (PA1804). <br> PXN was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PXN Antibody (PA1804) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1804-3-IHC-anti-paxillin-antibody.jpgAnti-Paxillin/PXN Antibody Picoband&reg; IHC analysis of PXN using anti-PXN antibody (PA1804). <br> PXN was detected in a frozen section of Rat Kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PXN Antibody (PA1804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1804-4-IF-anti-paxillin-antibody.jpgAnti-Paxillin/PXN Antibody Picoband&reg; ICC analysis of PXN using anti-PXN antibody (PA1804). <br> PXN was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-PXN Antibody (PA1804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1804-pxn-primary-antibody-if-testing-5.jpgAnti-Paxillin/PXN Antibody Picoband&reg; IF analysis of PXN using anti-PXN antibody (PA1804). <br> PXN was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-PXN Antibody (PA1804) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1804-pxn-primary-antibody-fcm-testing-6.pngAnti-Paxillin/PXN Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-PXN antibody (PA1804). <br>Overlay histogram showing A549 cells stained with PA1804 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PXN Antibody (PA1804, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1804-pxn-primary-antibody-if-testing-7.jpgAnti-Paxillin/PXN Antibody Picoband&reg; IF analysis of PXN using anti-PXN antibody (PA1804). <br> PXN was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PXN Antibody (PA1804) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arg2-antibody-pa1805-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1805-arg2-primary-antibodies-wb-testing-1.jpgAnti-Arginase-2, mitochondrial Arg2 Antibody Picoband&reg; Western blot analysis of ARG2 using anti-ARG2 antibody (PA1805). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human PC-3 whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG2 antigen affinity purified polyclonal antibody (Catalog # PA1805) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG2 at approximately 38 kDa. The expected band size for ARG2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1805-arg2-primary-antibodies-ihc-testing-2.jpgAnti-Arginase-2, mitochondrial Arg2 Antibody Picoband&reg; IHC analysis of ARG2 using anti-ARG2 antibody (PA1805). <br> ARG2 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ARG2 Antibody (PA1805) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aurora-b-antibody-pa1806-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1806-aurkb-primary-antibodies-wb-testing-1.jpgAnti-Aurora B/AURKB Antibody Picoband&reg;Western blot analysis of AURKB using anti-AURKB antibody (PA1806). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: rat spleen tissue lysates,<br> Lane 6: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AURKB antigen affinity purified polyclonal antibody (PA1806) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AURKB at approximately 39 kDa. The expected band size for AURKB is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmi1-antibody-pa1807-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1807-bmi1-primary-antibodies-wb-testing-1.jpgAnti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband&reg;Western blot analysis of BMI1 using anti-BMI1 antibody (PA1807). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 antigen affinity purified polyclonal antibody (PA1807) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BMI1 at approximately 40 kDa. The expected band size for BMI1 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-crkl-antibody-pa1808-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1808-crkl-primary-antibodies-wb-testing-1.jpgAnti-Crk-like protein CrkL Antibody Picoband&reg; Western blot analysis of CRKL using anti-CRKL antibody (PA1808). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human U20S whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat thymus tissue lysates,<br> Lane 6: rat brain tissue lysates,<br> Lane 7: mouse thymus tissue lysates,<br> Lane 8: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRKL antigen affinity purified polyclonal antibody (Catalog # PA1808) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRKL at approximately 39 kDa. The expected band size for CRKL is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1808-crkl-primary-antibodies-fcm-testing-2.jpgAnti-Crk-like protein CrkL Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-CRKL antibody (PA1808). <br> Overlay histogram showing U251 cells stained with PA1808 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRKL Antibody (PA1808, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-daxx-antibody-pa1809-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1809-daxx-primary-antibody-wb-testing-1.jpgAnti-Daxx Antibody Picoband&reg; Western blot analysis of DAXX using anti-DAXX antibody (PA1809). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Ramos whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: mouse testis tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DAXX antigen affinity purified polyclonal antibody (Catalog # PA1809) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DAXX at approximately 115 kDa. The expected band size for DAXX is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1809-daxx-primary-antibody-if-testing-2.jpgAnti-Daxx Antibody Picoband&reg; IF analysis of DAXX using anti-DAXX antibody (PA1809) and anti-Beta Tubulin antibody (M01857-3).<br> DAXX was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-DAXX Antibody (PA1809) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e2f2-antibody-pa1810-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1810-1-WB-anti-e2f2-antibody.jpgAnti-Transcription factor E2F2 E2F2 Antibody Picoband&reg;Anti-E2F2 antibody&#44; PA1810&#44; Western blotting<br>All lanes: Anti E2F2 (PA1810) at 0.5ug/ml<br> Lane 1: Rat Lung Tissue Lysate at 50ug<br> Lane 2: Rat Heart Tissue Lysate at 50ug<br> Lane 3: Rat Brain Tissue Lysate at 50ug<br> Lane 4: Rat Kidney Tissue Lysate at 50ug<br> Lane 5: HELA Whole Cell Lysate at 40ug<br> Lane 6: COLO320 Whole Cell Lysate at 40ug<br> Lane 7: A549 Whole Cell Lysate at 40ug<br> Lane 8: MCF-7 Whole Cell Lysate at 40ug<br> Lane 9: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 48KD<br> Observed bind size: 48KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e2f6-antibody-pa1811-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1811-1-WB-anti-e2f6-antibody.jpgAnti-Transcription factor E2F6 E2F6 Antibody Picoband&reg;Anti-E2F6 antibody&#44; PA1811&#44; Western blotting<br>All lanes: Anti E2F6 (PA1811) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: COLO320 Whole Cell Lysate at 40ug<br> Lane 3: A549 Whole Cell Lysate at 40ug<br> Lane 4: MCF-7 Whole Cell Lysate at 40ug<br> Lane 5: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 32KD<br> Observed bind size: 32KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp70-antibody-pa1813-boster.html2026-03-24T05:03:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1813-anxa3-primary-antibodies-wb-testing-1.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; Western blot analysis of HSP70 using anti-HSP70 antibody (PA1813). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: human A549 whole cell lysates,<br> Lane 6: human PC-3 whole cell lysates,<br> Lane 7: human 293T whole cell lysates,<br> Lane 8: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP70 antigen affinity purified polyclonal antibody (Catalog # PA1813) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1813-anxa3-primary-antibodies-wb-testing-2.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; Western blot analysis of HSP70 using anti-HSP70 antibody (PA1813). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat RH35 whole cell lysates,<br> Lane 5: mouse liver tissue lysates,<br> Lane 6: mouse kidney tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP70 antigen affinity purified polyclonal antibody (Catalog # PA1813) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1813-4-IHC-anti-hsp70-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg;Anti-Hsp70 antibody&#44; PA1813&#44; IHC(F)<br>IHC(F):Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1813-2-IHC-anti-hsp70-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg;Anti-Hsp70 antibody&#44; PA1813&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1813-3-IF-anti-hsp70-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg;Anti-Hsp70 antibody&#44; PA1813&#44; ICC<br>ICC: A549 Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hspa2-antibody-pa1814-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1814-1-WB-anti-hspa2-antibody.jpgAnti-HSPA2 Antibody Picoband&reg; Western blot analysis of HSPA2 using anti-HSPA2 antibody (PA1814). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Testis Tissue Lysate&#44; <br> Lane 2: A549 Cell Lysate&#44; <br> Lane 3: MCF-7 Cell Lysate&#44;<br> Lane 4: HELA Cell Lysate . <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody (Catalog # PA1814) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1814-2.jpgAnti-HSPA2 Antibody Picoband&reg; Western blot analysis of HSPA2 using anti-HSPA2 antibody (PA1814). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse spleen Tissue Lysate&#44; <br> Lane 2: mouse testis tissue Lysate&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody (Catalog # PA1814) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70KD. The expected band size for HSPA2 is at 70KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grp78-bip-antibody-pa1815-boster.html2026-03-26T05:19:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1815-hspa5-primary-antibodies-wb-testing-2.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Western blot analysis of HSPA5 using anti-HSPA5 antibody (PA1815). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human A431 whole cell lysates.<br> Lane 5: human Hela whole cell lysates,<br> Lane 6: human SiHa whole cell lysates,<br> Lane 7: human A549 whole cell lysates,<br> Lane 8: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody (PA1815) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSPA5 at approximately 78 kDa. The expected band size for HSPA5 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1815-2-IHC-anti-grp78-bip-antibody.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Anti-GRP78 BiP antibody&#44; PA1815&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1815-3-IHC-anti-grp78-bip-antibody.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Anti-GRP78 BiP antibody&#44; PA1815&#44; IHC(P)<br>IHC(P): Rat Cerebellum Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1815-4-IHC-anti-grp78-bip-antibody.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Anti-GRP78 BiP antibody&#44; PA1815&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1815-5-IF-anti-grp78-bip-antibody.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Anti-GRP78 BiP antibody&#44; PA1815&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1815-6-IHC-anti-grp78-bip-antibody.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Anti-GRP78 BiP antibody&#44; PA1815&#44; IHC(F)<br>IHC(F): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1815-hspa5-primary-antibodies-wb-testing-1.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Western blot analysis of HSPA5 using anti-HSPA5 antibody (PA1815). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: mouse testis tissue lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody (PA1815) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSPA5 at approximately 78 kDa. The expected band size for HSPA5 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1815-hspa5-primary-antibodies-wb-testing-1_1.pngAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg; Western blot analysis of HSPA5 using anti-HSPA5 antibody (PA1815). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1-6: Different segments of the porcine large intestine and small intestine lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified monoclonal antibody (Catalog # PA1815) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with ChemiDoc MP system. A specific band was detected for HSPA5 at approximately 70-78 kDa. The expected band size for HSPA5 is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsc70-antibody-pa1816-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1816-hspa8-primary-antibodies-wb-testing-1.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; Western blot analysis of Hsc70 using anti-Hsc70 antibody (PA1816). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 3: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsc70 antigen affinity purified polyclonal antibody (Catalog # PA1816) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsc70 at approximately 71 kDa. The expected band size for Hsc70 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1816-hspa8-primary-antibodies-ihc-testing-4.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg;IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (PA1816). <br>HSC70/HSPA8 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSC70/HSPA8 Antibody (PA1816) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1816-2-IHC-anti-hsc70-antibody.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; IHC analysis of Hsc70 using anti-Hsc70 antibody (PA1816). <br> Hsc70 was detected in paraffin-embedded section of Human Lung Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsc70 Antibody (PA1816) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1816-3_1.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; IHC analysis of Hsc70 using anti-Hsc70 antibody (PA1816). <br> Hsc70 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Hsc70 Antibody (PA1816) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1816-hspa8-primary-antibodies-fcm-testing-5.pngAnti-Hsc70/HSPA8 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-Hsc70 antibody (PA1816). <br>Overlay histogram showing HL-60 cells stained with PA1816 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsc70 Antibody (PA1816, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1816-hspa8-primary-antibodies-if-testing-4.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; IF analysis of Hsc70 using anti- Hsc70 antibody (PA1816). <br> Hsc70 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hsc70 Antibody (PA1816) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hyal2-antibody-pa1817-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1817-1-WB-anti-hyal2-antibody.jpgAnti-Hyaluronidase-2 HYAL2 Antibody Picoband&reg;Anti-HYAL2 antibody&#44; PA1817&#44; Western blotting<br>All lanes: Anti HYAL2 (PA1817) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: SMMC Whole Cell Lysate at 40ug<br> Lane 3: COLO320 Whole Cell Lysate at 40ug<br> Lane 4: MCF-7 Whole Cell Lysate at 40ug<br> Lane 5: HT1080 Whole Cell Lysate at 40ug<br> Predicted bind size: 54KD<br> Observed bind size: 54KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irf2-antibody-pa1818-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1818-1-WB-anti-irf2-antibody.jpgAnti-Interferon regulatory factor 2 IRF2 Antibody Picoband&reg;Anti-IRF2 antibody&#44; PA1818&#44; Western blotting<br>All lanes: Anti IRF2 (PA1818) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 39KD<br> Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irf3-antibody-pa1819-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1819-elife-94898-fig1-v1.jpgAnti-Interferon regulatory factor 3 IRF3 Antibody Picoband&reg;A genetic compensation response model for RIG loss. (A) Loss of RIG-I in vertebrates. Each branch tip represents one species. Red lines show lineages where loss of RIG-I. The red circle indicates the occurrence of an independent loss event. Branch lengths scale in millions of years (MYA). (B) Comparative analysis of gene synteny of RIG-I in vertebrate genomes. (C and D) SCRV-RNA and VSV-RNA were extracted from SCRV and VSV virus and dephosphorylated them with Calf Intestinal Alkaline Phosphatase (CIAP). Then, MKC cells were transfected with SCRV-RNA and SCRV-RNA +CIAP, and DF-1 cells were transfected with VSV-RNA and VSV-RNA +CIAP. IRF3 dimerization was analyzed by native gel electrophoresis. (E) MKC cells were transfected with different concentrations of SCRV-RNA and SCRV-RNA +CIAP, then the expression of IFN-1 was detected by qRT-PCR. As a control, the expression level of IFN-1 in MKC cells transfected with the same volume of water was set to ‘1’. (F) Predicted protein structures of MDA5 and LGP2 of M. miiuy and RIG-I of H. sapiens. (G) The expression of MDA5 and LGP2 plasmids of M. miiuy were detected by western Blot. (H) MKC cells were co-transfected with MDA5 plasmids and LGP2 plasmids for 24 hr and then treated with SCRV (MOI = 5) for 24 hr. The expression of IFN-1 was determined by qPCR. (I) MKC cells were co-transfected with MDA5 plasmids and si-LGP2 for 24 hr and then treated with SCRV (MOI = 5) for 24 hr. The expression of LGP2 and IFN-1 was determined by qPCR. (J) A genetic compensation response model for RIG loss. All data presented as the means ± SE from three independent triplicated experiments. **, p<0.01, as determined by Student’s t test. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://elifesciences.org/articles/94898'>39347580</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1819-irf3-primary-antibodies-wb-testing-1.jpgAnti-Interferon regulatory factor 3 IRF3 Antibody Picoband&reg; Western blot analysis of IRF3 using anti-IRF3 antibody (PA1819). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF3 antigen affinity purified polyclonal antibody (Catalog # PA1819) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF3 at approximately 55 kDa. The expected band size for IRF3 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-9-antibody-pa1820-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1820-1-WB-anti-kallikrein-9-antibody.jpgAnti-Kallikrein 9/KLK9 Antibody Picoband&reg;Anti-Kallikrein 9 antibody&#44; PA1820&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: A431 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-10-antibody-pa1821-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1821-1-WB-anti-kallikrein-10-antibody.jpgAnti-Kallikrein 10/KLK10 Antibody Picoband&reg;Anti-Kallikrein 10 antibody&#44; PA1821&#44; Western blotting<br>All lanes: Anti Kallikrein 10 (PA1821) at 0.5ug/ml<br> Lane 1: Rat Ovary Tissue Lysate at 50ug<br> Lane 2: Rat Testis Tissue Lysate at 50ug<br> Lane 3: Rat Spleen Tissue Lysate at 50ug<br> Lane 4: Rat Liver Tissue Lysate at 50ug<br> Lane 5: 22RV Whole Cell Lysate at 40ug<br> Lane 6: SROV Whole Cell Lysate at 40ug<br> Lane 7: HELA Whole Cell Lysate at 40ug<br> Lane 8: MCF-7 Whole Cell Lysate at 40ug<br> Lane 9: MM231 Whole Cell Lysate at 40ug<br> Predicted bind size: 37KD<br> Observed bind size: 37KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lamp1-antibody-pa1822-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1822-13287_2018_1081_fig2_html.pngAnti-LAMP1 Antibody Picoband&reg;SGJ increased the concentration of H + in lysosomes, and up-regulated LAMP1 and LAMP2 protein level. a Acridine orange staining for young (PDL 5) and senescent (PDL 20) BMSCs. Acidic vacuoles declined with age as shown in the results. Twenty-micromolar SGJ treatments for 1, 3, 6, and 12 h significantly restored the amount of acidic vacuoles (magnification × 200). b SGJ promoted lysosomal acidification. Lysosensor™ Green DND-189 was used to sense the changes of the concentration of H + in lysosomes, and quantification. BMSCs were treated with 20 nM Baf-A1 or 20 μM SGJ for 24 h. The changes of the red fluorescence reflect changes in lysosomal pH. c Western blot analysis of LAMP1 and LAMP2 protein levels with β-actin as a loading control, and quantification. BMSCs were treated with 20 μM SGJ for 6, 12, 24 and 48 h. (*, p < 0.05; **, p < 0.01, results were expressed as means ± SEM, n = 3) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-018-1081-0'>30526663</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1822-lamp1-primary-antibodies-wb-testing-1.jpgAnti-LAMP1 Antibody Picoband&reg; Western blot analysis of LAMP1 using anti-LAMP1 antibody (PA1822). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: mouse brain tissue lysates,<br> Lane 5: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP1 antigen affinity purified polyclonal antibody (Catalog # PA1822) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAMP1 at approximately 90-120 kDa (Glycosylation). The expected band size for LAMP1 is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mpg-antibody-pa1825-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1825-1-WB-anti-mpg-apng-antibody.jpgAnti-MPG Antibody Picoband&reg;Anti-MPG antibody&#44; PA1825&#44; Western blotting<br>All lanes: Anti MPG (PA1825) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 33KD<br>Observed bind size: 33KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myoglobin-antibody-pa1826-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1826-1-WB-anti-myoglobin-antibody.jpgAnti-Myoglobin/MB Antibody Picoband&reg;Anti-Myoglobin antibody&#44; PA1826&#44; Western blotting<br>Lane 1: Human Placenta Tissue Lysate<br>Lane 2: Human Placenta Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myoglobin-antibody-pa1827-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1827-mb-primary-antibodies-ihc-testing-4.jpgAnti-Myoglobin/MB Antibody Picoband&reg;IHC analysis of Myoglobin/MB using anti-Myoglobin/MB antibody (PA1827). <br>Myoglobin/MB was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin/MB Antibody (PA1827) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1827-myoglobin-primary-antibodies-wb-testing-1.jpgAnti-Myoglobin/MB Antibody Picoband&reg; Western blot analysis of Myoglobin using anti-Myoglobin antibody (PA1827). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: rat heart tissue lysates, <br> Lane 3: rat skeletal muscle tissue lysates, <br> Lane 4: mouse heart tissue lysates, <br> Lane 5: mouse skeletal muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Myoglobin antigen affinity purified polyclonal antibody (PA1827) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Myoglobin at approximately 17 kDa. The expected band size for Myoglobin is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1827-myoglobin-primary-antibodies-ihc-testing-2.jpgAnti-Myoglobin/MB Antibody Picoband&reg; IHC analysis of Myoglobin using anti-Myoglobin antibody (PA1827). <br> Myoglobin was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin Antibody (PA1827) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1827-myoglobin-primary-antibodies-ihc-testing-3.jpgAnti-Myoglobin/MB Antibody Picoband&reg; IHC analysis of Myoglobin using anti-Myoglobin antibody (PA1827). <br> Myoglobin was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Myoglobin Antibody (PA1827) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nm23a-antibody-pa1829-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1829-nme1-primary-antibodies-wb-testing-1.jpgAnti-NM23A/NME1 Antibody Picoband&reg; Western blot analysis of NM23A using anti-NM23A antibody (PA1829). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A antigen affinity purified polyclonal antibody (Catalog # PA1829) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A at approximately 17 kDa. The expected band size for NM23A is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1829-2-IHC-anti-nm23a-antibody.jpgAnti-NM23A/NME1 Antibody Picoband&reg; IHC analysis of NM23A using anti-NM23A antibody (PA1829).<br> NM23A was detected in paraffin-embedded section of Rat Cerebellum tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NM23A Antibody (PA1829) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1829-3-IF-anti-nm23a-antibody.jpgAnti-NM23A/NME1 Antibody Picoband&reg; IHC analysis of NM23A using anti-NM23A antibody (PA1829).<br> NM23A was detected in immunocytochemical section of HELA Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-NM23A Antibody (PA1829) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1829-nme1-primary-antibodies-fc-testing-4.jpgAnti-NM23A/NME1 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-NME1 antibody (PA1829). <br>Overlay histogram showing Hela cells stained with PA1829 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NME1 Antibody (PA1829&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1829-5.jpgAnti-NM23A/NME1 Antibody Picoband&reg; IHC analysis of NM23A using anti-NM23A antibody (PA1829). <br> NM23A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NM23A Antibody (PA1829) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nme2-antibody-pa1830-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1830-2-IHC-anti-nme2-nm23-h2-antibody.jpgAnti-NME2 Antibody Picoband&reg;Anti-NME2 antibody&#44; PA1830&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1830-3-IHC-anti-nme2-nm23-h2-antibody.jpgAnti-NME2 Antibody Picoband&reg;Anti-NME2 antibody&#44; PA1830&#44; IHC(P)<br>IHC(P): Rat Cerebellum Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1830-4-IF-anti-nme2-nm23-h2-antibody.jpgAnti-NME2 Antibody Picoband&reg;Anti-NME2 antibody&#44; PA1830&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1830-1_1.jpgAnti-NME2 Antibody Picoband&reg; Western blot analysis of NME2 using anti-NME2 antibody (PA1830). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse heart tissue lysates, <br> Lane 4: mouse brain tissue lysates, <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NME2 antigen affinity purified polyclonal antibody (Catalog # PA1830) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NME2 at approximately 17KD. The expected band size for NME2 is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1830-5.jpgAnti-NME2 Antibody Picoband&reg; IHC analysis of NME2 using anti-NME2 antibody (PA1830). <br> NME2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NME2 Antibody (PA1830) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1830-6.jpgAnti-NME2 Antibody Picoband&reg; IF analysis of NME2 using anti-NME2 antibody (PA1830). <br> NME2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NME2 Antibody (PA1830) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1830-7.jpgAnti-NME2 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-NME2 antibody (PA1830). <br>Overlay histogram showing HL-60 cells stained with PA1830 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NME2 Antibody (PA1830, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-notch4-antibody-pa1831-boster.html2026-03-24T05:03:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1831-1-WB-anti-notch4-antibody.jpgAnti-NOTCH4 Antibody Picoband&reg;Anti-NOTCH4 antibody&#44; PA1831&#44; Western blotting<br>All lanes: Anti NOTCH4 (PA1831) at 0.5ug/ml<br>Lane 1: A549 Whole Cell Lysate at 40ug<br>Lane 2: SMMC Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 210KD<br>Observed bind size: 210KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lox-1-antibody-pa1832-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1832-olr1-primary-antibodies-wb-testing-1.jpgAnti-LOX 1/OLR1 Antibody Picoband&reg; Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1832). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human prostatic carcinoma tissue (PCT) lysates, <br> Lane 2: human cervical carcinoma tissue (CCAT) lysates, <br> Lane 3: human gallbladder carcinoma tissue (GBCT) lysates, <br> Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1832) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 fo r 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lox-1-antibody-pa1833-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1833-olr1-primary-antibodies-wb-testing-1.jpgAnti-LOX 1/OLR1 Antibody Picoband&reg; Western blot analysis of LOX 1/OLR1 using anti-LOX 1/OLR1 antibody (PA1833). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human prostatic carcinoma tissue (PCT) lysates, <br> Lane 2: human cervical carcinoma tissue (CCAT) lysates, <br> Lane 3: human gallbladder carcinoma tissue (GBCT) lysates, <br> Lane 4: human intrahepatic cholangiocarcinom paracancerous tissue (ICCP) lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX 1/OLR1 antigen affinity purified polyclonal antibody (Catalog # PA1833) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX 1/OLR1 at approximately 48-52 kDa. The expected band size for LOX 1/OLR1 is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-2-antibody-pa1834-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1834-1-WB-anti-peroxiredoxin-2-antibody.jpgAnti-Peroxiredoxin 2/PRDX2 Antibody Picoband&reg;Anti-Peroxiredoxin 2 antibody&#44; PA1834&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Kidney Tissue Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br>Lane 5: 293T Cell Lysate<br>Lane 6: A549 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-3-antibody-pa1835-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1835-prdx3-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; Western blot analysis of PRDX3 using anti-PRDX3 antibody (PA1835). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human HEK293 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX3 antigen affinity purified polyclonal antibody (Catalog # PA1835) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX3 at approximately 26 kDa. The expected band size for PRDX3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1835-2-ihc-anti-peroxiredoxin-3-antibody.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of PRDX3 using anti-PRDX3 antibody (PA1835). <br> PRDX3 was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRDX3 Antibody (PA1835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1835-3-IHC-anti-peroxiredoxin-3-antibody.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of PRDX3 using anti-PRDX3 antibody (PA1835). <br> PRDX3 was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRDX3 Antibody (PA1835) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1835-prdx3-primary-antibodies-if-testing-4.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IF analysis of PRDX3 using anti-PRDX3 antibody (PA1835). <br> PRDX3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRDX3 Antibody (PA1835) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1835-prdx3-primary-antibodies-fcm-testing-5.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-PRDX3 antibody (PA1835). <br> Overlay histogram showing K562 cells stained with PA1835 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDX3 Antibody (PA1835, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-3-antibody-pa1836-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1836-prdx3-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; Western blot analysis of PRDX3 using anti-PRDX3 antibody (PA1836). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat liver tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX3 antigen affinity purified polyclonal antibody (Catalog # PA1836) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX3 at approximately 26 kDa. The expected band size for PRDX3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1836-2-IHC-anti-peroxiredoxin-3-antibody.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of PRDX3 using anti-PRDX3 antibody (PA1836). <br> PRDX3 was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRDX3 Antibody (PA1836) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1836-3-ihc-anti-peroxiredoxin-3-antibody.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of PRDX3 using anti-PRDX3 antibody (PA1836). <br> PRDX3 was detected in a paraffin-embedded section of Human Mammary Cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRDX3 Antibody (PA1836) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1836-prdx3-primary-antibodies-if-testing-4.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IF analysis of PRDX3 using anti-PRDX3 antibody (PA1836). <br> PRDX3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRDX3 Antibody (PA1836) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1836-prdx3-primary-antibodies-fcm-testing-5.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-PRDX3 antibody (PA1836). <br> Overlay histogram showing Hela cells stained with PA1836 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDX3 Antibody (PA1836, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-4-antibody-pa1837-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1837-1-WB-anti-peroxiredoxin-4-antibody.jpgAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg;Anti-Peroxiredoxin 4 antibody&#44; PA1837&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Lung Tissue Lysate<br>Lane 3: Rat Liver Tissue Lysate <br>Lane 4: Rat Kidney Tissue Lysate<br>Lane 5: HELA Cell Lysate <br>Lane 6: 293T Cell Lysate<br>Lane 7: MCF-7 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-5-antibody-pa1838-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1838-1-WB-anti-peroxiredoxin-5-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg;Anti-Peroxiredoxin 5 antibody&#44; PA1838&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Lung Tissue Lysate<br>Lane 3: Rat Liver Tissue Lysate<br>Lane 4: Rat Kidney Tissue Lysate<br>Lane 5: HELA Cell Lysate <br>Lane 6: 293T Cell Lysate<br>Lane 7: MCF-7 Cell Lysate<br> Lane 8: A549 Cell Lysate https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1838-2-IHC-anti-peroxiredoxin-5-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg;Anti-Peroxiredoxin 5 antibody&#44; PA1838&#44; IHC(P)<br>IHC(P): Human Prostatic Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1838-3-IHC-anti-peroxiredoxin-5-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg;Anti-Peroxiredoxin 5 antibody&#44; PA1838&#44; IHC(P)<br>IHC(P): Rat Liver Tissue<br><br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sdhc-antibody-pa1839-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1839-2-IHC-anti-sdhc-antibody.jpgAnti-SDHC Antibody Picoband&reg;Anti-SDHC antibody&#44; PA1839&#44; IHC(P)<br>IHC(P): Rat Gaster Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1839-3-IHC-anti-sdhc-antibody.jpgAnti-SDHC Antibody Picoband&reg;Anti-SDHC antibody&#44; PA1839&#44; IHC(P)<br>IHC(P): Human Gastric Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1839-1_1-WB-anti-sdhc-antibody.jpgAnti-SDHC Antibody Picoband&reg;Anti-SDHC antibody&#44; PA1839&#44; Western blotting<br>All lanes: Anti SDHC (PA1839) at 0.5ug/ml<br> Lane 1: Rat Liver Tissue Lysate at 50ug<br> Lane 2: Mouse Liver Tissue Lysate at 50ug<br> Lane 3: HELA Whole Cell Lysate at 40ug<br> Predicted bind size: 19KD<br> Observed bind size: 19KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat5a-antibody-pa1840-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1840-stat5a-primary-antibodies-wb-testing-1.jpgAnti-STAT5a Antibody Picoband&reg; Western blot analysis of STAT5A using anti-STAT5A antibody (PA1840). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human Raji whole cell lysates, <br> Lane 4: human placenta tissue lysates, <br> Lane 5: rat thymus tissue lysates, <br> Lane 6: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A antigen affinity purified polyclonal antibody (Catalog # PA1840) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5A at approximately 95 kDa. The expected band size for STAT5A is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1840-2.pngAnti-STAT5a Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-STAT5A antibody (PA1840). <br> Overlay histogram showing U20S cells stained with PA1840 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5A Antibody (PA1840&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1840-3.pngAnti-STAT5a Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-STAT5A antibody (PA1840). <br> Overlay histogram showing Jurkat cells stained with PA1840 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5A Antibody (PA1840&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat5b-antibody-pa1841-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1841-1-WB-anti-stat5b-antibody.jpgAnti-STAT5b Antibody Picoband&reg;Anti-STAT5b antibody&#44; PA1841&#44; Western blotting<br>Lane 1: Rat Intestine Tissue Lysate<br>Lane 2: Rat Kidney Tissue Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: A549 Cell Lysate<br>Lane 5: MM231 Cell Lysate<br>Lane 6: COLO320 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1841-2-IHC-anti-stat5b-antibody.jpgAnti-STAT5b Antibody Picoband&reg;Anti-STAT5b antibody&#44; PA1841&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dr5-antibody-pa1842-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1842-1-WB-anti-dr5-antibody.jpgAnti-DR5/TNFRSF10B Antibody Picoband&reg;Anti-DR5 antibody&#44; PA1842&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: MM231 Cell Lysate<br>Lane 3: SGC Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1842-2-IHC-anti-dr5-antibody.jpgAnti-DR5/TNFRSF10B Antibody Picoband&reg;Anti-DR5 antibody&#44; PA1842&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-arrestin-2-antibody-pa1845-boster.html2026-03-24T05:03:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1845-1-IHC-anti-beta-arrestin-2-antibody.jpgAnti-Beta Arrestin 2/ARRB2 Antibody Picoband&reg;Anti-Beta Arrestin 2 antibody&#44; PA1845&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1845-2-IHC-anti-beta-arrestin-2-antibody.jpgAnti-Beta Arrestin 2/ARRB2 Antibody Picoband&reg;Anti-Beta Arrestin 2 antibody&#44; PA1845&#44; IHC(P)<br>IHC(P): Rat Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1845-3_1-WB-anti-beta-arrestin-2-antibody.jpgAnti-Beta Arrestin 2/ARRB2 Antibody Picoband&reg;Anti-Beta Arrestin 2 antibody&#44; PA1845&#44; Western blotting<br>All lanes: Anti ARRB2 (PA1845) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Predicted bind size: 46KD<br>Observed bind size: 46KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beclin-1-antibody-pa1847-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1847-1-WB-anti-beclin-1-antibody.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg;Anti-Beclin 1 antibody&#44; PA1847&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: SW620 Cell Lysate<br>Lane 3: PANC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-b-raf-antibody-pa1848-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1848-braf-primary-antibodies-wb-testing-1.jpgAnti-B Raf/BRAF Antibody Picoband&reg;Western blot analysis of BRAF using anti-BRAF antibody (PA1848). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MOLT4 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRAF antigen affinity purified polyclonal antibody (PA1848) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BRAF at approximately 84 kDa. The expected band size for BRAF is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1848-braf-primary-antibodies-fcm-testing-1.jpgAnti-B Raf/BRAF Antibody Picoband&reg;Flow Cytometry analysis of U937 cells using anti-BRAF antibody (PA1848). <br> Overlay histogram showing U937 cells stained with PA1848 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRAF Antibody (PA1848, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-3-antibody-pa1849-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1849-1-WB-anti-caspase-3-antibody.jpgAnti-Caspase 3/CASP3 Antibody Picoband&reg;Anti-CASP3 antibody&#44; PA1849&#44; Western blotting<br>All lanes: Anti CASP3 (PA1849) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: Rat Liver Tissue Lysate at 50ug<br>Lane 3: Rat Thymus Tissue Lysate at 50ug<br>Lane 4: MCF-7 Whole Cell Lysate at 40ug<br>Lane 5: SMMC Whole Cell Lysate at 40ug<br>Lane 6: HT1080 Whole Cell Lysate at 40ug<br>Predicted bind size: 31KD<br>Observed bind size: 31KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd1d-antibody-pa1850-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1850-1-WB-anti-cd1d-antibody.jpgAnti-CD1d Antibody Picoband&reg;Anti-CD1d antibody&#44; PA1850&#44; Western blotting<br>Lane 1: COLO320 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1850-2-IHC-anti-cd1d-antibody.jpgAnti-CD1d Antibody Picoband&reg;Anti-CD1d antibody&#44; PA1850&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-coxsackie-adenovirus-receptor-antibody-pa1852-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1852-ataxin_3-primary-antibodies-wb-testing-2.jpgAnti-Coxsackie Adenovirus Receptor/CXADR Antibody Picoband&reg; Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody (PA1852). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysate&#44; <br> Lane 2: rat heart tissue lysates&#44;<br> Lane 3: mouse liver tissue lysates&#44; <br> Lane 4: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody (Catalog # PA1852) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1852-ataxin_3-primary-antibodies-wb-testing-1.jpgAnti-Coxsackie Adenovirus Receptor/CXADR Antibody Picoband&reg; Western blot analysis of Coxsackie Adenovirus Receptor using anti-Coxsackie Adenovirus Receptor antibody (PA1852). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates&#44; <br> Lane 2: human Hela whole cell lysates&#44; <br> Lane 3: human HepG2 whole cell lysates&#44; <br> Lane 4: human Caco-2 whole cell lysates&#44; <br> Lane 5: human U-937whole cell lysates(negative)&#44;<br> Lane 6: human THP-1whole cell lysates(negative).<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Coxsackie Adenovirus Receptor antigen affinity purified polyclonal antibody (Catalog # PA1852) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Coxsackie Adenovirus Receptor at approximately 50KD. The expected band size for Coxsackie Adenovirus Receptor is at 40KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fatty-acid-binding-protein-5-antibody-pa1853-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1853-fabp5-primary-antibodies-wb-testing-1_1.jpgAnti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband&reg; Western blot analysis of FABP5 using anti-FABP5 antibody (PA1853). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates,<br> Lane 2: rat intestine tissue lysates.<br> Lane 3: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP5 antigen affinity purified polyclonal antibody (Catalog # PA1853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP5 at approximately 15 kDa. The expected band size for FABP5 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1853-fabp5-primary-antibodies-ihc-testing-2.jpgAnti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband&reg; IHC analysis of FABP5 using anti-FABP5 antibody (PA1853). <br> FABP5 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP5 Antibody (PA1853) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1853-fabp5-primary-antibodies-ihc-testing-3.jpgAnti-Fatty Acid Binding Protein 5/FABP5 Antibody Picoband&reg; IHC analysis of FABP5 using anti-FABP5 antibody (PA1853). <br> FABP5 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FABP5 Antibody (PA1853) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fes-antibody-pa1854-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1854-1-WB-anti-fes-antibody.jpgAnti-FES Antibody Picoband&reg;Anti-FES antibody&#44; PA1854&#44; Western blotting<br>All lanes: Anti FES (PA1854) at 0.5ug/ml<br>WB: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 93KD<br>Observed bind size: 93KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fes-antibody-pa1854-1-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1854-1-1-WB-anti-fes-antibody.jpgAnti-FES Antibody Picoband&reg;Anti-FES antibody&#44; PA1854-1&#44; All Western blotting<br>All lanes: Anti-FES(PA1854-1) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: 293T Whole Cell Lysate at 40ug<br> Lane 3: SKOV Whole Cell Lysate at 40ug<br> Lane 4: A549 Whole Cell Lysate at 40ug<br> Predicted bind size: 93KD<br> Observed bind size: 93KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hyal3-antibody-pa1856-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1856-1-WB-anti-hyal3-antibody.jpgAnti-Hyaluronidase-3 HYAL3 Antibody Picoband&reg;Anti-HYAL3 antibody&#44; PA1856&#44; Western blotting<br>Lane 1: 22RV Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: V20S Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1856-2-IHC-anti-hyal3-antibody.jpgAnti-Hyaluronidase-3 HYAL3 Antibody Picoband&reg;Anti-HYAL3 antibody&#44; PA1856&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-orai1-antibody-pa1857-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1857-1-WB-anti-orai1-antibody.jpgAnti-Orai1 Antibody Picoband&reg;Anti-Orai1 antibody&#44; PA1857&#44; Western blotting<br>All lanes: Anti Orai1 (PA1857) at 0.5ug/ml<br>WB: SKOV Whole Cell Lysate at 40ug<br>Predicted bind size: 33KD<br>Observed bind size: 50KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ptch2-antibody-pa1859-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1859-1-WB-anti-ptch2-patched-2-antibody.jpgAnti-PTCH2 Antibody Picoband&reg;Anti-PTCH2 antibody&#44; PA1859&#44; Western blotting<br>WB: HELA Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1859-41281756.pngAnti-PTCH2 Antibody Picoband&reg;TGF-β1/SMAD3 promotes the nuclear translocation and expression of GLI2. (A) SMAD3 and p-SMAD3 protein levels were obtained by western blot upon primary PSC treated with single or combined TGF-β1 and SIS3 treatment for 4 days (n = 4). β-actin was used to normalize protein levels. (B) mRNA levels of Gli1, Smo, Ptch1, and Ptch2 in PSCs following 24-hour treatment with 10 ng/mL TGF-β1 (n = 3). (C) Representative immunofluorescence images showing expression levels of GLI1 (red), SMO (green), and PTCH2 (yellow) in PSCs treated with 10 ng/mL TGF-β1 for 72 hours. Nuclei were stained with DAPI (blue). Scale bar: 20 μm. Fluorescence intensity of GLI1, PTCH2, and SMO was quantified (n = 4). All data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01,∗∗∗p < 0.001,∗∗∗∗p < 0.0001. ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ijbs.com/v21p6978.htm'>41281756</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-shp2-antibody-pa1860-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1860-ptpn11-primary-antibodies-wb-testing-1.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg; Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (PA1860). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human CCRF-CEM whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates.<br> Lane 6: rat C6 whole cell lysates.<br> Lane 7: mouse brain tissue lysates.<br> Lane 8: mouse 3T3-L1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2/PTPN11 antigen affinity purified polyclonal antibody (Catalog # PA1860) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2/PTPN11 at approximately 68 kDa. The expected band size for SHP2/PTPN11 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1860-ptpn11-primary-antibodies-ihc-testing-2.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg; IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (PA1860). <br> SHP2/PTPN11 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHP2/PTPN11 Antibody (PA1860) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1860-ptpn11-primary-antibodies-if-testing-3.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg; IF analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (PA1860). <br> SHP2/PTPN11 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SHP2/PTPN11 Antibody (PA1860) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1860-ptpn11-primary-antibodies-fcm-testing-4.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-SHP2/PTPN11 antibody (PA1860). <br> Overlay histogram showing CACO-2 cells stained with PA1860 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHP2/PTPN11 Antibody (PA1860, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rip2-antibody-pa1861-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1861-1-WB-anti-rip2-antibody.jpgAnti-RIP2/RIPK2 Antibody Picoband&reg;Anti-RIP2 antibody&#44; PA1861&#44; Western blotting<br>All lanes: Anti RIP2 (PA1861) at 0.5ug/ml<br> Lane 1: A549 Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: PANC Whole Cell Lysate at 40ug<br> Lane 4: COLO320 Whole Cell Lysate at 40ug<br> Predicted bind size: 61KD<br> Observed bind size: 61KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-splicing-factor-1-antibody-pa1864-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1864-sf1-primary-antibodies-wb-testing-1.jpgAnti-splicing factor 1/SF1 Antibody Picoband&reg; Western blot analysis of splicing factor 1 using anti-splicing factor 1 antibody (PA1864). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human THP-1 whole cell lysates, <br> Lane 4: rat PC-12 whole cell lysates, <br> Lane 5: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-splicing factor 1 antigen affinity purified polyclonal antibody (Catalog # PA1864) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for splicing factor 1 at approximately 68-80 kDa. The expected band size for splicing factor 1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1864-2-IHC-anti-splicing-factor-1-antibody.jpgAnti-splicing factor 1/SF1 Antibody Picoband&reg; IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (PA1864). <br> splicing factor 1 was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-splicing factor 1 Antibody (PA1864) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1864-3_1.jpgAnti-splicing factor 1/SF1 Antibody Picoband&reg; IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (PA1864).<br> splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-splicing factor 1 Antibody (PA1864) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1864-4-IHC-anti-splicing-factor-1-antibody.jpgAnti-splicing factor 1/SF1 Antibody Picoband&reg; IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (PA1864). <br> splicing factor 1 was detected in a frozen section of Rat Spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-splicing factor 1 Antibody (PA1864) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1864-5-if-anti-splicing-factor-1-antibody.jpgAnti-splicing factor 1/SF1 Antibody Picoband&reg; ICC analysis of splicing factor 1 using anti-splicing factor 1 antibody (PA1864). <br> splicing factor 1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-splicing factor 1 Antibody (PA1864) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1864-sf1-primary-antibodies-if-testing-6.jpgAnti-splicing factor 1/SF1 Antibody Picoband&reg; IF analysis of splicing factor 1 using anti-splicing factor 1 antibody (PA1864). <br> splicing factor 1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-splicing factor 1 Antibody (PA1864) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1864-sf1-primary-antibodies-fcm-testing-7.jpgAnti-splicing factor 1/SF1 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-splicing factor 1 antibody (PA1864). <br>Overlay histogram showing 293T cells stained with PA1864 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-splicing factor 1 Antibody (PA1864, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sp4-antibody-pa1865-boster.html2026-03-24T05:03:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1865-sp4-primary-antibodies-wb-testing-1.jpgAnti-Transcription factor Sp4 Antibody Picoband&reg; Western blot analysis of Sp4 using anti-Sp4 antibody (PA1865). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp4 antigen affinity purified polyclonal antibody (Catalog # PA1865) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sp4 at approximately 82 kDa. The expected band size for Sp4 is at 82 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tgf-beta-receptor-ii-antibody-pa1866-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1866-1-WB-anti-tgfbr2-tgf-beta-receptor-ii-antibody.jpgAnti-TGF beta Receptor II/TGFBR2 Antibody Picoband&reg;Anti-TGF beta Receptor II antibody&#44; PA1866&#44; Western blotting<br>All lanes: Anti TGF beta Receptor II (PA1866) at 0.5ug/ml<br>Lane 1: HEPG2 Whole Cell Lysate at 40ug<br> Lane 2: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 65KD<br>Observed bind size: 65KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1866-fig-3-1x.jpgAnti-TGF beta Receptor II/TGFBR2 Antibody Picoband&reg;Hsa_circ_0009096 sponges miR-370-3p to modulate TGFBR2 expression in LX-2 cells. (A) LX-2 cells were transfected with hsa_circ_0009096 siRNAs for 24 h and then RT-qPCR was used to assess the interference effect of siRNAs ( n = 3 biological replicates per group). (B) Relative mRNA expression of potential target genes of hsa_circ_0009096 ( SMAD4, TGFB2, TGFBR2, and THBS1 ) in LX-2 cells after transfection with hsa_circ_ 0009096 siRNA3 for 24 h were detected via RT-qPCR ( n = 3 biological replicates per group). (C) The expression of miR-21-5p and miR-370-3p in LX-2 cells transfected with hsa_circ_ 0009096 siRNA3 for 24 h were analyzed by the RT-qPCR ( n = 3 biological replicates per group). (D) The interaction between hsa_circ_0009096 and miR-370-3p in 293T cells was confirmed by the dual luciferase reporter assay ( n = 3 biological replicates per group). NC: negative control; TGFBR2: TGF beta receptor 2; SMAD4: Mothers Against Decepentaplegic Homolog 4; TGFB2: Transforming Growth Factor Beta 2; THBS1: Thrombospondin 1; NS, not significant ( p > 0.05), * p < 0.05, ** p < 0.01. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/17356/'>38766485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1866-fig-4-1x.jpgAnti-TGF beta Receptor II/TGFBR2 Antibody Picoband&reg;Hsa_circ_0009096 silencing suppressed TGF-β1-induced HSC proliferation and fibrosis. LX-2 cells were transfected with siNC or hsa_circ_0009096 siRNA3 for 24 h, followed by treatment without or with 2 ng/mL rhTGF-β1 for 24 h. (A–D) The miRNA/mRNA expression of a-SMA, COL1A1, TGFBR2, and miR-370-3p were detected by RT-qPCR ( n = 3 biological replicates per group). (E–F) The cell cycle progression and the apoptosis of LX-2 cells were assessed by flow cytometry ( n = 3 biological replicates per group). (G) Alterations of a-SMA and COL1A1 protein abundances in LX-2 cells were evaluated by Immunocytochemistry ( n = 3 biological replicates per group). (H) The protein expression of TGFBR2, COL1A1, and a-SMA was examined by western blotting ( n = 3 biological replicates per group). NC: negative control; TGFBR2: TGF beta receptor 2; COL1A1: collagen 1A1; α-SMA: alpha-smooth muscle actin; NS, not significant ( p > 0.05), * p < 0.05, ** p < 0.01. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/17356/'>38766485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1866-fig-5-1x.jpgAnti-TGF beta Receptor II/TGFBR2 Antibody Picoband&reg;MiR-370-3p mediated hsa_circ_0009096-regulated proliferation and fibrosis of TGF-β1-treated HSCs. LX-2 cells were transfected with siNC+NC-inhibitor, hsa_circ_0009096 siRNA3+NC-inhibitor, and hsa_circ_0009096 siRNA3+miR-370-3p-inhibitor, respectively, for 24 h, followed by treatment with 2 ng/mL rhTGF-β1 for 24 h. (A–D) The miRNA/mRNA expression of miR-370-3p, TGFBR2, COL1A1, and a-SMA were detected by RT-qPCR ( n = 3 biological replicates per group). (E–F) The cell cycle progression and the apoptosis of LX-2 cells were assessed by flow cytometry ( n = 3 biological replicates per group). (G) Alterations of a-SMA and COL1A1 protein abundances in LX-2 cells were evaluated by Immunocytochemistry ( n = 3 biological replicates per group). (H) The protein expression of TGFBR2, COL1A1, and a-SMA was examined by western blotting ( n = 3 biological replicates per group). NC: negative control; TGFBR2: TGF beta receptor 2; COL1A1: collagen 1A1; α-SMA: alpha-smooth muscle actin; NS, not significant ( p > 0.05), * p < 0.05, ** p < 0.01. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/17356/'>38766485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1866-41598_2022_9581_fig8_html.pngAnti-TGF beta Receptor II/TGFBR2 Antibody Picoband&reg;Bioinformatics analysis of DEPs. All 522 DEPs underwent the analysis of subcellular localization ( A ), the enrichment analysis of KEGG signaling pathways ( B ) and Gene Ontology annotation ( C ). ( D ) The DEPs in the “Proteoglycans in cancer” KEGG pathway. ( E ) Western blot analysis of TGF-β2, SMAD2, and p-SMAD2 in PANC-1 cells treated with or without rhoifolin. GAPDH was used as internal reference. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-022-09581-3'>35383226</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcg1-antibody-pa1868-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1868-1-WB-anti-abcg1-antibody.jpgAnti-ABCG1 Antibody Picoband&reg;Anti-ABCG1 antibody&#44; PA1868&#44; Western blotting<br>All lanes: Anti ABCG1 (PA1868) at 0.5ug/ml<br> Lane 1: U87 Whole Cell Lysate at 40ug<br> Lane 2: SMMC Whole Cell Lysate at 40ug<br> Lane 3: HELA Whole Cell Lysate at 40ug<br> Lane 4: COLO320 Whole Cell Lysate at 40ug<br> Lane 5: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 75KD<br> Observed bind size: 75KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcrp-abcg2-antibody-pa1869-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1869-abcg2-primary-antibodies-wb-testing-1.jpgAnti-BCRP/ABCG2 Antibody Picoband&reg; Western blot analysis of BCRP/ABCG2 using anti-BCRP/ABCG2 antibody (PA1869). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCRP/ABCG2 antigen affinity purified polyclonal antibody (Catalog # PA1869) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCRP/ABCG2 at approximately 75 kDa. The expected band size for BCRP/ABCG2 is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcrp-abcg2-antibody-pa1870-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1870-1-WB-anti-bcrp-abcg2-antibody.jpgAnti-BCRP/ABCG2 Antibody Picoband&reg;Anti-BCRP/ABCG2 antibody&#44; PA1870&#44; Western blotting<br>All lanes: Anti BCRP/ABCG2 (PA1870) at 0.5ug/ml<br> Lane 1: Rat Liver Tissue Lysate at 50ug<br> Lane 2: Rat Spleen Tissue Lysate at 50ug<br> Lane 3: Rat Kidney Tissue Lysate at 50ug<br> Lane 4: Mouse Spleen Tissue Lysate at 50ug<br> Predicted bind size: 72KD<br> Observed bind size: 72KD https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-beta-actin-antibody-pa1872-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1872-actb-primary-antibodies-wb-testing-1.jpgAnti-beta Actin/ACTB Antibody Picoband&reg; Western blot analysis of beta Actin/ACTB using anti-beta Actin/ACTB antibody (PA1872). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Actin/ACTB antigen affinity purified polyclonal antibody (Catalog # PA1872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Actin/ACTB at approximately 42 kDa. The expected band size for beta Actin/ACTB is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1872-actb-primary-antibodies-wb-testing-2.jpgAnti-beta Actin/ACTB Antibody Picoband&reg; Western blot analysis of beta Actin/ACTB using anti-beta Actin/ACTB antibody (PA1872). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: zebrafish tissue lysates,<br> Lane 2: chicken heart tissue lysates,<br> Lane 3: chicken liver tissue lysates,<br> Lane 4: chicken brain tissue lysates,<br> Lane 5: monkey heart tissue lysates,<br> Lane 6: monkey lung tissue lysates,<br> Lane 7: monkey kidney tissue lysates,<br> Lane 8: monkey liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Actin/ACTB antigen affinity purified polyclonal antibody (Catalog # PA1872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Actin/ACTB at approximately 42 kDa. The expected band size for beta Actin/ACTB is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd163-antibody-pa1874-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1874-cd163-primary-antibodies-wb-testing-1.jpgAnti-CD163 Antibody Picoband&reg; Western blot analysis of CD163 using anti-CD163 antibody (PA1874). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # PA1874) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150 kDa. The expected band size for CD163 is at 125 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd163-antibody-pa1874-1-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1874-1-13046_2020_1637_fig1_html.pngAnti-CD163 Antibody Picoband&reg;Decreased CPEB3 in human CRC correlates with low CD86 + TAM content and high CD163 + TAM content (a) The expression of CPEB3 and CD86 in 82 pairs of CRC tissues and adjacent non-tumor tissues was detected using qRT-PCR. Correlation between CPEB3 and CD86 or CD163 expression levels in 82 colorectal cancer tissues; error bars, SEM. (b) The protein expression of CPEB3, CD68, CD86, and CD163 in a human colorectal cancer tissue array was detected by IHC staining. Representative photos are shown (400× magnification). The number of CD68 + , CD86 + and CD163 + cells per high-power field in tissues from colorectal cancer patients with different levels of CPEB3 expression; error bars, SEM. (c) Schema for an in vitro model of stably transfected CRC cells co-cultured with TAMs. (d) Flow cytometry was used to explore the surface expression of CD86 and CD163 in SW480-Ctrl/CPEB3 and HCT116-Ctrl/CPEB3 cells; error bars, SEM. (e) Flow cytometry was used to explore the surface expression of CD86 and CD163 in LoVo-shCtrl/shCPEB3 and RKO-shCtrl/shCPEB3 cells; error bars, SEM. (f) We measured the expression of the respective inflammatory cytokines in cell culture supernatants of TAMs co-cultured HCT116-Ctrl/CPEB3 cells using ProcartaPlex combinable panels; error bars, SEM. (g) We measured the expression of the respective inflammatory cytokines in cell culture supernatants of TAM-co-cultured LoVo-shCtrl/shCPEB3 cells using ProcartaPlex combinable panels; error bars, SEM; ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-020-01637-4'>32653013</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1874-1-13046_2020_1637_fig5_html.pngAnti-CD163 Antibody Picoband&reg;CPEB3 modulates CCL2 secretion in CRC cell supernatants to regulate TAM polarization (a) We measured the expression of the respective inflammatory cytokines in cell culture supernatants of HCT116-Ctrl/CPEB3 cells by ProcartaPlex combinable panels; error bars, SEM. (b) We measured the expression of the respective inflammatory cytokines in the supernatants of LoVo-shCtrl/shCPEB3 cells by ProcartaPlex combinable panels; error bars, SEM. (c) THP-1 macrophages were co-cultured with LoVo-shCtrl/shCPEB3 with or without CCL2-neutralizing antibody (1 μg/mL) for 24 h. Flow cytometry was used to explore the surface expression of CD86 and CD163 in the differentiated macrophages; error bars, SEM. (d) THP-1 macrophages were co-cultured with RKO-shCtrl/shCPEB3 cells with or without a CCL2-neutralizing antibody (1 μg/mL) for 24 h. Flow cytometry was used to explore the surface expression of CD86 and CD163 in the differentiated macrophages. Error bars, SEM. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-020-01637-4'>32653013</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1874-1-13046_2020_1637_fig6_html.pngAnti-CD163 Antibody Picoband&reg;CPEB3 attenuates tumorigenesis and TAM polarization in vivo (a) Schematic of the procedure for separating tumor cells and TAMs. (b) HCT116 cells were stably infected with Ctrl and CPEB3 lentivirus, and LoVo cells were stably infected with shCtrl and shCPEB3 sequences. Tumorigenesis assay of Balb/c nude mice subcutaneously injected with HCT116-Ctrl/CPEB3 cells and LoVo-shCtrl/shCPEB3 cells ( n = 20). Representative photos of tumors from mice in various groups. ( c) IHC staining of Ki67 positive cells was counted per high-power field (PHF), while E-cadherin and vimentin expression scores were counted in tumor tissues in a mouse xenograft model; error bars, SEM. (d) The mice with intra-spleen injection of LoVo-shCtrl/shCPEB3 cells were treated with tocilizumab (5 mg/kg) weekly via intraperitoneally injection. The number of liver metastatic sites (indicated by arrows) was counted under the microscope; error bars, SEM. (e) Macrophages were separated from murine tumor tissues using Percoll-layered liquid. Surface expression of CD86 and CD163 was detected in macrophages using flow cytometry. The percentage of CD86 + or CD163 + cells in macrophages was reported using error bars and SEM. (f) Expression of JAK1, pJAK1, STAT3, and pSTAT3 in the tumor tissues of the two groups were analyzed by western blot analysis. (g) Schematic overview of the mechanisms by which CPEB3 modulate TAM polarization and inhibit colorectal cancer EMT. ** P < 0.01; *** P < 0.001; **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-020-01637-4'>32653013</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1874-1-cd163-primary-antibodies-wb-testing-1.jpgAnti-CD163 Antibody Picoband&reg; Western blot analysis of CD163 using anti-CD163 antibody (PA1874-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD163 antigen affinity purified polyclonal antibody (Catalog # PA1874-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD163 at approximately 150 kDa. The expected band size for CD163 is at 125 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1874-1-cd163-primary-antibodies-ihc-testing-2.jpgAnti-CD163 Antibody Picoband&reg; IHC analysis of CD163 using anti-CD163 antibody (PA1874-1). <br> CD163 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (PA1874-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1874-1-cd163-primary-antibodies-ihc-testing-3.jpgAnti-CD163 Antibody Picoband&reg; IHC analysis of CD163 using anti-CD163 antibody (PA1874-1). <br> CD163 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD163 Antibody (PA1874-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd1a-antibody-pa1875-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1875-cd1a-primary-antibodies-wb-testing-1.jpgAnti-CD1a Antibody Picoband&reg; Western blot analysis of CD1a using anti-CD1a antibody (PA1875). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1a antigen affinity purified polyclonal antibody (Catalog # PA1875) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1a at approximately 37 kDa. The expected band size for CD1a is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cullin-2-antibody-pa1877-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1877-1-WB-anti-cullin-2-antibody.jpgAnti-Cullin 2/CUL2 Antibody Picoband&reg;Anti-Cullin 2 antibody&#44; PA1877&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant human Cul2&#44; 46.5KD (162aa tag+L150-Y394)<br>Lane 1: Recombinant Human Cul2 Protein 10ng <br>Lane 2: Recombinant Human Cul2 Protein 5ng <br>Lane 3: Recombinant Human Cul2 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1877-3-WB-anti-cullin-2-antibody.jpgAnti-Cullin 2/CUL2 Antibody Picoband&reg;Anti-Cullin 2 antibody&#44; PA1877&#44; Western blotting<br>WB: Rat Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1877-5-WB-anti-cullin-2-antibody.jpgAnti-Cullin 2/CUL2 Antibody Picoband&reg;Anti-Cullin 2 antibody&#44; PA1877&#44; Western blotting<br>Lane 1: A431 Cell Lysate<br>Lane 2: SMMC Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: COLO320 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mck10-antibody-pa1878-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1878-ddr1-primary-antibodies-wb-testing-1.jpgAnti-MCK10/DDR1 Antibody Picoband&reg; Western blot analysis of DDR1 using anti-DDR1 antibody (PA1878). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human CACO-2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog # PA1878) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 120 kDa. The expected band size for DDR1 is at 101 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddr2-antibody-pa1879-boster.html2026-03-27T05:07:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1879-ddr2-primary-antibodies-wb-testing-1.jpgAnti-DDR2 Antibody Picoband&reg; Western blot analysis of DDR2 using anti-DDR2 antibody (PA1879). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates, <br> Lane 2: rat C6 whole cell lysates, <br> Lane 3: mouse NIH/3T3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # PA1879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR2 at approximately 120KD. The expected band size for DDR2 is at 97KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erbb-3-antibody-pa1880-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1880-1_1.jpgAnti-ErbB 3/ERBB3 Antibody Picoband&reg; Western blot analysis of ERBB3 using anti-ERBB3 antibody (PA1880). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates<br> Lane 2: human K562 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # PA1880) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180KD. The expected band size for ERBB3 is at 148KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erbb-4-antibody-pa1881-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1881-1-WB-anti-erbb-4-antibody.jpgAnti-ErbB 4/ERBB4 Antibody Picoband&reg;Anti-ErbB 4 antibody&#44; PA1881&#44; Western blotting<br>Lane 1: A549 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1881-3-WB-anti-erbb-4-antibody.jpgAnti-ErbB 4/ERBB4 Antibody Picoband&reg;Anti-ErbB 4 antibody&#44; PA1881&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant human ERRB4&#44; 40.6KD (162aa tag+M1-P200)<br>Lane 1: Recombinant Human ERRB4 Protein 10ng <br>Lane 2: Recombinant Human ERRB4 Protein 5ng <br>Lane 3: Recombinant Human ERRB4 Protein 2.5ng <br>Lane 4: Recombinant Human ERRB4 Protein 1.25nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1881-4-WB-anti-erbb-4-antibody.jpgAnti-ErbB 4/ERBB4 Antibody Picoband&reg;Anti-ErbB 4 antibody&#44; PA1881&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: U87 Cell Lysate<br>Lane 3: NEURO Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fer-antibody-pa1882-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1882-2-IHC-anti-fer-antibody.jpgAnti-Tyrosine-protein kinase Fer FER Antibody Picoband&reg;Anti-FER antibody&#44; PA1882&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1882-3-IHC-anti-fer-antibody.jpgAnti-Tyrosine-protein kinase Fer FER Antibody Picoband&reg;Anti-FER antibody&#44; PA1882&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1882-1_1-WB-anti-fer-antibody.jpgAnti-Tyrosine-protein kinase Fer FER Antibody Picoband&reg;Anti-FER antibody&#44; PA1882&#44; Western blotting<br>All lanes: Anti FER (PA1882) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: Rat Testis Tissue Lysate at 50ug<br>Lane 3: Rat Ovary Tissue Lysate at 50ug<br>Predicted bind size: 95KD<br>Observed bind size: 95KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-flavin-containing-monooxygenase-4-antibody-pa1883-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1883-1-WB-anti-flavin-containing-monooxygenase-4-antibody.jpgAnti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband&reg;Anti-Flavin containing monooxygenase 4 antibody&#44; PA1883&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Mouse Liver Tissue Lysate<br>Lane 3: SMMC Cell Lysate<br>Lane 4: HEPA Cell Lysate<br>Lane 5: A431 Cell Lysate<br>Lane 6: MCF-7 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1883-2-IHC-anti-flavin-containing-monooxygenase-4-antibody.jpgAnti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband&reg;Anti-Flavin containing monooxygenase 4 antibody&#44; PA1883&#44; IHC(P)<br>IHC(P): Rat Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1883-fmo4-primary-antibodies-ihc-testing-3.jpgAnti-Flavin containing monooxygenase 4/FMO4 Antibody Picoband&reg;IHC analysis of FMO4 using anti-FMO4 antibody (PA1883). <br>FMO4 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FMO4 Antibody (PA1883) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gata2-antibody-pa1884-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1884-1-WB-anti-gata2-antibody.jpgAnti-GATA2 Antibody Picoband&reg;Anti-GATA2 antibody&#44; PA1884&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human GATA2&#44; 38.3KD (162aa tag+ M1-G200)<br>Lane 1: Recombinant Human GATA2 Protein 10ng <br>Lane 2: Recombinant Human GATA2 Protein 5ng <br>Lane 3: Recombinant Human GATA2 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glutaredoxin-2-antibody-pa1885-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1885-glrx2-primary-antibodies-wb-testing-1.jpgAnti-Glutaredoxin 2/GLRX2 Antibody Picoband&reg; Western blot analysis of GLRX2 using anti-GLRX2 antibody (PA1885). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: rat lung tissue lysates,<br> Lane 3: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLRX2 antigen affinity purified polyclonal antibody (Catalog # PA1885) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLRX2 at approximately 18 kDa. The expected band size for GLRX2 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1885-2-IHC-anti-glutaredoxin-2-antibody.jpgAnti-Glutaredoxin 2/GLRX2 Antibody Picoband&reg; IHC analysis of Glutaredoxin 2 using anti-Glutaredoxin 2 antibody (PA1885). <br> Glutaredoxin 2 was detected in paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Glutaredoxin 2 Antibody (PA1885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1885-3_1.jpgAnti-Glutaredoxin 2/GLRX2 Antibody Picoband&reg; IHC analysis of Glutaredoxin 2 using anti-Glutaredoxin 2 antibody (PA1885). <br> Glutaredoxin 2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Glutaredoxin 2 Antibody (PA1885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1885-4_1.jpgAnti-Glutaredoxin 2/GLRX2 Antibody Picoband&reg; IHC analysis of Glutaredoxin 2 using anti-Glutaredoxin 2 antibody (PA1885). <br> Glutaredoxin 2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Glutaredoxin 2 Antibody (PA1885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bone-sialoprotein-antibody-pa1887-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1887-ibsp-primary-antibodies-wb-testing-1.jpgAnti-Bone Sialoprotein/IBSP Antibody Picoband&reg; Western blot analysis of IBSP using anti-IBSP antibody (PA1887). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat cartilage tissue lysates,<br> Lane 2: mouse cartilage tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IBSP antigen affinity purified polyclonal antibody (Catalog # PA1887) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IBSP at approximately 65 kDa. The expected band size for IBSP is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1887-1-ihc-anti-bone-sialoprotein-antibody.jpgAnti-Bone Sialoprotein/IBSP Antibody Picoband&reg; IHC analysis of IBSP using anti-IBSP antibody (PA1887). <br> IBSP was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IBSP Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1887-3.jpgAnti-Bone Sialoprotein/IBSP Antibody Picoband&reg; IHC analysis of Bone Sialoprotein using anti-Bone Sialoprotein antibody (PA1887). <br> Bone Sialoprotein was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bone Sialoprotein Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klf3-antibody-pa1888-boster.html2026-03-24T05:03:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1888-1-WB-anti-klf3-antibody.jpgAnti-Krueppel-like factor 3 KLF3 Antibody Picoband&reg;Anti-KLF3 antibody&#44; PA1888&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: HELA Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klf5-antibody-pa1889-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1889-klf5-primary-antibodies-wb-testing-1.jpgAnti-Krueppel-like factor 5 KLF5 Antibody Picoband&reg; Western blot analysis of KLF5 using anti-KLF5 antibody (PA1889). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLF5 antigen affinity purified polyclonal antibody (Catalog # PA1889) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLF5 at approximately 60 kDa. The expected band size for KLF5 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klf8-antibody-pa1890-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1890-1-WB-anti-klf8-antibody.jpgAnti-Krueppel-like factor 8 KLF8 Antibody Picoband&reg;Anti-KLF8 antibody&#44; PA1890&#44; Western blotting<br>Lane 1: SMMC Cell Lysate<br>Lane 2: 293T Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-jnk1-antibody-pa1892-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1892-1-WB-anti-jnk1-antibody.jpgAnti-JNK1/MAPK8 Antibody Picoband&reg;Anti-JNK1 antibody&#44; PA1892&#44; Western blotting<br>WB: HT1080 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p107-antibody-pa1894-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1894-1-WB-anti-p107-antibody.jpgAnti-p107/RBL1 Antibody Picoband&reg;Anti-p107 antibody&#44; PA1894&#44; Western blotting<br>Lane 1: 239T Cell Lysate<br>Lane 2: JURKAT Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1894-2-IHC-anti-p107-antibody.jpgAnti-p107/RBL1 Antibody Picoband&reg;Anti-p107 antibody&#44; PA1894&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1894-3-IHC-anti-p107-antibody.jpgAnti-p107/RBL1 Antibody Picoband&reg;Anti-p107 antibody&#44; PA1894&#44; IHC(P)<br>IHC(P): Rat Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-shc-antibody-pa1897-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1897-1-WB-anti-shc-antibody.jpgAnti-SHC/SHC1 Antibody Picoband&reg;Anti-SHC antibody&#44; PA1897&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant human SHC1&#44; 35.0KD(162aa tag+D424-P578)<br>Lane 1: Recombinant Human SHC1 Proteins 10ng<br>Lane 2: Recombinant Human SHC1 Proteins 5ng<br>Lane 3: Recombinant Human SHC1 Proteins 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1897-3-IHC-anti-shc-antibody.jpgAnti-SHC/SHC1 Antibody Picoband&reg;Anti-SHC antibody&#44; PA1897&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1897-4-IHC-anti-shc-antibody.jpgAnti-SHC/SHC1 Antibody Picoband&reg;Anti-SHC antibody&#44; PA1897&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1897-5-IHC-anti-shc-antibody.jpgAnti-SHC/SHC1 Antibody Picoband&reg;Anti-SHC antibody&#44; PA1897&#44; IHC(F)<br>IHC(F): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1897-6-IHC-anti-shc-antibody.jpgAnti-SHC/SHC1 Antibody Picoband&reg;Anti-SHC antibody&#44; PA1897&#44; IHC(F)<br>IHC(F): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1897-7-IF-anti-shc-antibody.jpgAnti-SHC/SHC1 Antibody Picoband&reg;Anti-SHC antibody&#44; PA1897&#44; ICC<br>ICC: A549 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1897-2_2.jpgAnti-SHC/SHC1 Antibody Picoband&reg; Western blot analysis of SHC using anti-SHC antibody (PA1897). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysate&#44; <br> Lane 2: human Hela whole cell lysate&#44; <br> Lane 3: human HepG2 whole cell lysate&#44; <br> Lane 4: human Jurkat whole cell lysate&#44; <br> Lane 5: rat C6 whole cell lysate&#44; <br> Lane 6: mouse thymus tissue lysate&#44; <br> Lane 7: mouse RAW246.7 whole cell lysate&#44; <br> Lane 8: mouse NIH3T3 whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC antigen affinity purified polyclonal antibody (Catalog # PA1897) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for SHC at approximately 46&#44; 52&#44; 66KD. The expected band size for SHC are at 46&#44; 52&#44; 66KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smad2-antibody-pa1898-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1898-smad2-primary-antibodies-wb-testing-1.jpgAnti-Smad2 Antibody Picoband&reg; Western blot analysis of SMAD2 using anti-SMAD2 antibody (PA1898). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human MDA-MB-453 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # PA1898) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60 kDa. The expected band size for SMAD2 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1898-smad2-primary-antibodies-ihc-testing-2.jpgAnti-Smad2 Antibody Picoband&reg; IHC analysis of SMAD2 using anti-SMAD2 antibody (PA1898). <br> SMAD2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SMAD2 Antibody (PA1898) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1898-smad2-primary-antibodies-ihc-testing-3.jpgAnti-Smad2 Antibody Picoband&reg; ICC analysis of SMAD2 using anti-SMAD2 antibody (PA1898). <br> SMAD2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4 μg/ml rabbit anti-SMAD2 Antibody (PA1898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sod3-antibody-pa1899-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1899-1-WB-anti-sod3-ec-sod-antibody.jpgAnti-Superoxide Dismutase 3/SOD3 Antibody Picoband&reg;Anti-SOD3 antibody&#44; PA1899&#44; Western blotting<br>Lane 1: Human Placenta Tissue Lysate<br>Lane 2: A549 Cell Lysate<br>Lane 3: MM231 Cell Lysate<br>Lane 4: MCF-7 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1899-2-IHC-anti-sod3-ec-sod-antibody.jpgAnti-Superoxide Dismutase 3/SOD3 Antibody Picoband&reg;Anti-SOD3 antibody&#44; PA1899&#44; IHC(F)<br>IHC(F): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1899-3.jpgAnti-Superoxide Dismutase 3/SOD3 Antibody Picoband&reg; IHC analysis of SOD3 using anti-SOD3 antibody (PA1899). <br> SOD3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD3 Antibody (PA1899) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1899-4.jpgAnti-Superoxide Dismutase 3/SOD3 Antibody Picoband&reg; ICC analysis of SOD3 using anti-SOD3 antibody (PA1899). <br> SOD3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SOD3 Antibody (PA1899) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trif-antibody-pa1902-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1902-1-WB-anti-trif-antibody.jpgAnti-TRIF/TICAM1 Antibody Picoband&reg;Anti-TRIF antibody&#44; PA1902&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human TICAM1&#44; 44.7KD(162aa tag+ Q468-E712)<br>Lane 1: Recombinant Human TICAM1 Protein 10ng<br>Lane 2: Recombinant Human TICAM1 Protein 5ng<br>Lane 3: Recombinant Human TICAM1 Protein 2.5ng<br>Lane 4: Recombinant Human TICAM1 Protein 1.25nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1902-3-IHC-anti-trif-antibody.jpgAnti-TRIF/TICAM1 Antibody Picoband&reg;Anti-TRIF antibody&#44; PA1902&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1902-2_1.jpgAnti-TRIF/TICAM1 Antibody Picoband&reg; Western blot analysis of TRIF using anti-TRIF antibody (PA1902). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIF antigen affinity purified polyclonal antibody (Catalog # PA1902) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIF at approximately 110KD. The expected band size for TRIF is at 76KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-txnrd2-antibody-pa1903-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1903-1-WB-anti-txnrd2-trxr2-antibody.jpgAnti-TXNRD2 Antibody Picoband&reg;Anti-TXNRD2 antibody&#44; PA1903&#44; Western blotting<br>Lane 1: Rat Kidney Tissue Lysate<br>Lane 2: Rat Ovary Tissue Lysate<br>Lane 3: Rat Liver Tissue Lysate<br>Lane 4: SMMC Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1903-2-IHC-anti-txnrd2-trxr2-antibody.jpgAnti-TXNRD2 Antibody Picoband&reg;Anti-TXNRD2 antibody&#44; PA1903&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1903-3.jpgAnti-TXNRD2 Antibody Picoband&reg; IHC analysis of TXNRD2 using anti-TXNRD2 antibody (PA1903). <br> TXNRD2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TXNRD2 Antibody (PA1903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcsf-receptor-antibody-pa1905-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1905-1-WB-anti-csf1r-m-csfr-antibody.jpgAnti-MCSF Receptor/CSF1R Antibody Picoband&reg;Anti-MCSF Receptor antibody&#44; PA1905&#44; Western blotting<br>WB: Rat Intestine Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1905-2.jpgAnti-MCSF Receptor/CSF1R Antibody Picoband&reg; Western blot analysis of MCSF Receptor using anti-MCSF Receptor antibody (PA1905). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates<br> Lane 2: mouse RAW246.7 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCSF Receptor antigen affinity purified polyclonal antibody (Catalog # PA1905) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCSF Receptor at approximately 108KD. The expected band size for MCSF Receptor is at 108KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gcsf-receptor-antibody-pa1906-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1906-1_1-WB-anti-csf3r-g-csf-r-antibody.jpgAnti-GCSF Receptor/CSF3R Antibody Picoband&reg;Anti-GCSF Receptor antibody&#44; PA1906&#44; Western blotting<br>WB: Human Placenta Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp2e1-antibody-pa1909-boster.html2026-03-24T05:03:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1909-1_1.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband&reg; Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (PA1909). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates&#44; <br> Lane 2: mouse liver tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody (Catalog # PA1909) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 56KD. The expected band size for CYP2E1 is at 56KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1909-2_1.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband&reg; IHC analysis of CYP2E using anti-CYP2E antibody (PA1909). <br> CYP2E was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E Antibody (PA1909) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1909-3.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband&reg; IHC analysis of CYP2E using anti-CYP2E antibody (PA1909). <br> CYP2E was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E Antibody (PA1909) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dcc-antibody-pa1910-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1910-1-WB-anti-dcc-antibody.jpgAnti-Netrin receptor DCC DCC Antibody Picoband&reg;Anti-DCC antibody&#44; PA1910&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: SW620 Cell Lysate<br>Lane 5: COLO320 Cell Lysate<br>Lane 6: 293T Cell Lysate<br>Lane 7: HELA Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1910-2-IHC-anti-dcc-antibody.jpgAnti-Netrin receptor DCC DCC Antibody Picoband&reg;Anti-DCC antibody&#44; PA1910&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dll3-antibody-pa1912-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1912-1_1.jpgAnti-Delta-like protein 3 DLL3 Antibody Picoband&reg; Western blot analysis of DLL3 using anti-DLL3 antibody (PA1912). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates<br> Lane 2: mouse brain tissue lysates<br> Lane 3: human HEK293 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLL3 antigen affinity purified polyclonal antibody (Catalog # PA1912) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLL3 at approximately 65KD. The expected band size for DLL3 is at 65KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-flt-3ligand-antibody-pa1914-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1914-1_1.jpgAnti-Flt3 ligand/FLT3LG Antibody Picoband&reg; Western blot analysis of FLT3LG using anti-FLT3LG antibody (PA1914). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates&#44; <br> Lane 2: human Caco-2 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLT3LG antigen affinity purified polyclonal antibody (Catalog # PA1914) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLT3LG at approximately 30KD. The expected band size for FLT3LG is at 26KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fstl3-antibody-pa1915-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1915-1_2.jpgAnti-Follistatin-related protein 3 FSTL3 Antibody Picoband&reg; Western blot analysis of FSTL3 using anti-FSTL3 antibody (PA1915). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U2OS whole cell lysates&#44;<br> Lane 2: human Hela whole cell lysates&#44;<br> Lane 3: human A549 whole cell lysates&#44; <br> Lane 4: human PC-3 whole cell lysates&#44;<br> Lane 5: human HepG2 whole cell lysates&#44;<br> Lane 6: human K562 whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody (Catalog # PA1915) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1915-jah39761-fig-0002.pngAnti-Follistatin-related protein 3 FSTL3 Antibody Picoband&reg;Expression levels (NPX) of CLSTN2, HGF, SPON1, CRIM1, PLAUR, and FSTL3 in plasma from CTRL and patients with PAH. According to contemporary cardiac index measured by right heart catheterization at the time blood sample was drawn, patients with PAH were categorized as cRV (cardiac index >2.2 L/[min·m2]) and dRV (cardiac index ≤2.2 L/[min·m2]). Statistical analyses were performed using the Student's t test or 1‐way ANOVA followed by Tukey post hoc test. *P< .05, **P<0.01, ***P<0.001, and ****P<0.0001. CLSTN2 indicates calsyntenin 2; cRV, compensated right ventricle; CRIM1, cysteine rich transmembrane bone morphogenetic protein regulator 1; CTD‐PAH, connective tissue disease‐associated pulmonary arterial hypertension; CTRL, controls; dRV, decompensated right ventricle; FSTL3, follistatin‐like 3; HGF, hepatocyte growth factor; IPAH, idiopathic pulmonary hypertension; NPX, normalized protein expression; PAH, pulmonary arterial hypertension; PLAUR, plasminogen activator, urokinase receptor; and SPON1, spondin 1. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ahajournals.org/doi/abs/10.1161/JAHA.123.032888'>38874078</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1915-2_1.jpgAnti-Follistatin-related protein 3 FSTL3 Antibody Picoband&reg; Western blot analysis of FSTL3 using anti-FSTL3 antibody (PA1915). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates&#44;<br> Lane 2: rat testis tissue lysates&#44;<br> Lane 3: rat NRK whole cell lysates&#44; <br> Lane 4: mouse liver tissue lysates&#44;<br> Lane 5: mouse testis tissue lysates&#44;<br> Lane 6: mouse lung tissue lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody (Catalog # PA1915) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-iduronate-2-sulfatase-antibody-pa1917-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1917-1-WB-anti-ids-iduronate-2-sulfatase-antibody.jpgAnti-Iduronate 2 sulfatase/IDS Antibody Picoband&reg;Anti-Iduronate 2 sulfatase antibody&#44; PA1917&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: SMMC Cell Lysate<br>Lane 3: A549 Cell Lysate<br>Lane 4: MCF-7 Cell Lysate<br>Lane 5: COLO Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1917-2-IHC-anti-ids-iduronate-2-sulfatase-antibody.jpgAnti-Iduronate 2 sulfatase/IDS Antibody Picoband&reg;Anti-Iduronate 2 sulfatase antibody&#44; PA1917&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ki67-antibody-pa1918-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1918-1-WB-anti-ki67-antibody.jpgAnti-Ki67/MKI67 Antibody Picoband&reg;Anti-Ki67 antibody&#44; PA1918&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human Ki67 50.3KD (162aa tag+ K2967-I3256)<br>Lane 1: Recombinant Human Ki67 Protein 10ng <br>Lane 2: Recombinant Human Ki67 Protein 5ng <br>Lane 3: Recombinant Human Ki67 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-malt1-antibody-pa1920-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1920-malt1-primary-antibodies-wb-testing-1.jpgAnti-MALT1 Antibody Picoband&reg;Western blot analysis of MALT1 using anti-MALT1 antibody (PA1920). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse RAW264.7 whole cell lysates,<br> Lane 7: mouse ANA-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MALT1 antigen affinity purified polyclonal antibody (PA1920) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MALT1 at approximately 92 kDa. The expected band size for MALT1 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1920-malt1-primary-antibodies-ihc-testing-1.jpgAnti-MALT1 Antibody Picoband&reg;IHC analysis of MALT1 using anti-MALT1 antibody (PA1920). <br>MALT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MALT1 Antibody (PA1920) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1920-malt1-primary-antibodies-ihc-testing-2.jpgAnti-MALT1 Antibody Picoband&reg;IHC analysis of MALT1 using anti-MALT1 antibody (PA1920). <br>MALT1 was detected in a paraffin-embedded section of human lyphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MALT1 Antibody (PA1920) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1920-malt1-primary-antibodies-if-testing-1.jpgAnti-MALT1 Antibody Picoband&reg;IF analysis of MALT1 using anti-MALT1 antibody (PA1920). <br> MALT1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MALT1 Antibody (PA1920) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1920-malt1-primary-antibodies-fcm-testing-1.jpgAnti-MALT1 Antibody Picoband&reg;Flow Cytometry analysis of Jurkat cells using anti-MALT1 antibody (PA1920). <br> Overlay histogram showing Jurkat cells stained with PA1920 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MALT1 Antibody (PA1920, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek6-antibody-pa1921-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1921-1-WB-anti-mek6-antibody.jpgAnti-MEK6/MAP2K6 Antibody Picoband&reg;Anti-MEK6 antibody&#44; PA1921&#44; Western blotting<br>Lane 1: Rat Ovary Tissue Lysate<br>Lane 2: Rat Spleen Tissue Lysate<br>Lane 3: Rat Brain Tissue Lysate<br>Lane 4: Rat Heart Tissue Lysate<br>Lane 5: NIH3I3 Cell Lysate<br>Lane 6: HELA Cell Lysate<br>Lane 7: JRUKAT Cell Lysate <br>Lane 8: MCF-7 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek7-antibody-pa1922-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1922-map2k7-primary-antibodies-wb-testing-1.jpgAnti-MEK7/MAP2K7 Antibody Picoband&reg; Western blot analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody (PA1922). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: monkey COS-7 whole cell lysates, <br> Lane 3: rat skeletal muscle tissue lysates, <br> Lane 4: mouse skeletal muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK7/MAP2K7 antigen affinity purified polyclonal antibody (Catalog # PA1922) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK7/MAP2K7 at approximately 47 kDa. The expected band size for MEK7/MAP2K7 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1922-map2k7-primary-antibodies-fcm-testing-2.jpgAnti-MEK7/MAP2K7 Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-MEK7/MAP2K7 antibody (PA1922). <br>Overlay histogram showing Jurkat cells stained with PA1922 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK7/MAP2K7 Antibody (PA1922, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mekk1-antibody-pa1923-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1923-map3k1-primary-antibodies-wb-testing-1.jpgAnti-MEKK1/MAP3K1 Antibody Picoband&reg;Western blot analysis of MAP3K1 using anti-MAP3K1 antibody (PA1923). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: rat PC-12 whole cell lysates,<br> Lane 4: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K1 antigen affinity purified polyclonal antibody (PA1923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAP3K1 at approximately 164 kDa. The expected band size for MAP3K1 is at 164 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1923-map3k1-primary-antibodies-ihc-testing-1.jpgAnti-MEKK1/MAP3K1 Antibody Picoband&reg;IHC analysis of MAP3K1 using anti-MAP3K1 antibody (PA1923). <br> MAP3K1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAP3K1 Antibody (PA1923) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1923-map3k1-primary-antibodies-fcm-testing-1.jpgAnti-MEKK1/MAP3K1 Antibody Picoband&reg;Flow Cytometry analysis of SH-SY5Y cells using anti-MAP3K1 antibody (PA1923). <br> Overlay histogram showing SH-SY5Y cells stained with PA1923 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP3K1 Antibody (PA1923, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mekk3-antibody-pa1924-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1924-map3k3-primary-antibodies-wb-testing-1.jpgAnti-MEKK3/MAP3K3 Antibody Picoband&reg; Western blot analysis of MEKK3/MAP3K3 using anti-MEKK3/MAP3K3 antibody (PA1924). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Daudi whole cell lysates, <br> Lane 3: human A431 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK3/MAP3K3 antigen affinity purified polyclonal antibody (Catalog # PA1924) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK3/MAP3K3 at approximately 71 kDa. The expected band size for MEKK3/MAP3K3 is at 71 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mekk3-antibody-pa1925-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1925-1-IHC-anti-mekk3-antibody.jpgAnti-MEKK3/MAP3K3 Antibody Picoband&reg;Anti-MEKK3 antibody&#44; PA1925&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1925-2-IHC-anti-mekk3-antibody.jpgAnti-MEKK3/MAP3K3 Antibody Picoband&reg;Anti-MEKK3 antibody&#44; PA1925&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1925-3-WB-anti-mekk3-antibody.jpgAnti-MEKK3/MAP3K3 Antibody Picoband&reg;Anti-MEKK3 antibody&#44; PA1925&#44; Western blotting<br>All lanes: Anti MAP3K3 (PA1925) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 71KD<br>Observed bind size: 71KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-matrilin-3-antibody-pa1927-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1927-1-WB-anti-matrilin-3-antibody.jpgAnti-Matrilin 3/MATN3 Antibody Picoband&reg;Anti-Matrilin 3 antibody&#44; PA1927&#44; Western blotting<br>Lane 1: 293T Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: A549 Cell Lysate<br><br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-b-myb-antibody-pa1928-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1928-mybl2-primary-antibodies-wb-testing-1.jpgAnti-B MyB/MYBL2 Antibody Picoband&reg; Western blot analysis of MYBL2 using anti-MYBL2 antibody (PA1928). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYBL2 antigen affinity purified polyclonal antibody (Catalog # PA1928) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYBL2 at approximately 100 kDa. The expected band size for MYBL2 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1928-mybl2-primary-antibodies-ihc-testing-2.jpgAnti-B MyB/MYBL2 Antibody Picoband&reg; IHC analysis of MYBL2 using anti-MYBL2 antibody (PA1928). <br> MYBL2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYBL2 Antibody (PA1928) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1928-mybl2-primary-antibodies-ihc-testing-3.jpgAnti-B MyB/MYBL2 Antibody Picoband&reg; IHC analysis of MYBL2 using anti-MYBL2 antibody (PA1928). <br> MYBL2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYBL2 Antibody (PA1928) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1928-mybl2-primary-antibodies-ihc-testing-4.jpgAnti-B MyB/MYBL2 Antibody Picoband&reg; IHC analysis of MYBL2 using anti-MYBL2 antibody (PA1928). <br> MYBL2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MYBL2 Antibody (PA1928) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nadph-oxidase-4-antibody-pa1929-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1929-nox4-primary-antibodies-wb-testing-1_1.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband&reg;Western blot analysis of NOX4 using anti-NOX4 antibody (PA1929). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human BEAS-2B whole cell lysates,<br> Lane 2: human BEAS-2B whole cell lysates,<br> Lane 3: human U2OS whole cell lysates,<br> Lane 4: human SH-SY5Y whole cell lysates,<br> Lane 5: human Hela whole cell lysates,<br> Lane 6: human HepG2 whole cell lysates,<br> Lane 7: human PC-3 whole cell lysates,<br> Lane 8: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (PA1929) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOX4 at approximately 67 kDa. The expected band size for NOX4 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1929-nox4-primary-antibodies-wb-testing-2.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband&reg;Western blot analysis of NOX4 using anti-NOX4 antibody (PA1929). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX4 antigen affinity purified polyclonal antibody (PA1929) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOX4 at approximately 67 kDa. The expected band size for NOX4 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/n/o/nox4_bp12620_human_kidney.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband&reg;IHC analysis of NOX4 using anti-NOX4 antibody (PA1929). <br>NOX4 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOX4 Antibody (PA1929) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1929-ajtr0011-2925-f6.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband&reg;Expression of RAS components, NOXs and TH in brain nuclei and kidney measured by western-blot. A. Protein levels of AGT (a1) and AT1 receptors (a2) in brain nuclei measured by Western-blot. B. Protein levels of NOX2 (b1) and NOX4 (b2) in brain nuclei measured by Western-blot. C. Protein expression of TH in SFO, SON PVN (c1) and RVLM (c2) measured by western-blot. D. Protein levels of AGT, AT1, MCP-1 (d1) and Noxs (d2) in renal cortex homogenates measured by Western-blot. Data are expressed as the mean ± SD (n=6 in each group). * P <0.05 versus IG 0 mg/kg/d Los.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1929-ajtr0011-2925-f2.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband&reg;Brain RAS, oxidative stress and sympathetic activity were up-regulated in DM rats. A. AGT and AT1 receptors in SFO (a1), SON (a2) and PVN (a3) measured by immunohistochemistry. B. AGT and AT1 receptors in SFO (b1), SON (b2) and PVN (b3) measured by Western blot. C. Protein levels of NOX2 and NOX4 in SFO (c1), SON (c2) and PVN (c3) measured by Western-blot. D. Representative photographs of TH+c-fos positive cells in RVLM measured by immunohistochemistry. E. Protein levels of TH in RVLM measured by Western-blot. F. Protein levels of TH in SFO, SON, PVN measured by Western-blot. Data are expressed as the mean ± SD (n=15 in each group). * P <0.05 versus Non-DM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1929-fphar-15-1516111-g004.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband&reg;Patients with IMN exhibited increased serum levels of EVs, and oxidative stress enhanced EVs production in vitro . (A) TEM images displayed the morphology of EVs isolated from the serum of IMN patients. Scale bar = 100 nm. Patients with MCD served as controls. NTA images showed the distribution of EVs. (B) Representative images of immune colloidal gold electron microscopy demonstrated the presence of PLA2R on the surface of EVs, indicated by black dots. Scale bar = 200 nm. (C, D) Images from nano-flow cytometry and a corresponding statistical bar chart illustrated the proportion of PLA2R + EVs in the serum of IMN patients compared to those with MCD. (E) A positive correlation was observed between the proportion of PLA2R + EVs and serum aPLA2Rab levels. (F) Representative Western blot analyses showed the PLA2R expression levels of EVs isolated from the lung tissue of smokers and non-smokers. (G) The levels of oxidative stress, PLA2R expression, and EVs production in Beas-2B cells were examined after stimulation with LPS, with or without GW4869 intervention. Representative Western blot (G) and quantitative data (H–L) are presented. The expression levels of NOX4 (H) , NOX2 (I) , TSG101 (J) , CD63 (K) , and PLA2R (L) were significantly upregulated by LPS, while GSH reversed this upregulation. (* p < 0.05 versus control; # p < 0.05 versus LPS). (M–R) The levels of oxidative stress, PLA2R expression, and EVs production in Beas-2B cells stimulated by RM8785 were analyzed, with or without GW4869 intervention. Representative Western blot (M) and quantitative data (N–R) are presented. The expression levels of NOX4 (N) , NOX2 (O) , TSG101 (P) , CD63 (Q) , and PLA2R (R) were significantly upregulated by RM8785, while GW4869 effectively reversed this upregulation. (* p < 0.05 versus control; # p < 0.05 versus PM2.5).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1516111/full'>39744137</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1929-fphar-15-1516111-g002.jpgAnti-NADPH oxidase 4/NOX4 Antibody Picoband&reg;PM2.5 induces oxidative stress and PLA2R overexpression in bronchial epithelium. (A–F) Levels of oxidative stress, PLA2R expression, and EVs production in Beas-2B cells stimulated by LPS, with or without GSH intervention. Western blot (A) and quantitative data (B–F) are presented. (B, C) NOX2 and NOX4 are oxidative stress markers from the NADPH oxidase family. (D) PLA2R expression levels. (E, F) TSG101 and CD63 are markers for EVs. All are significantly upregulated by LPS, while GSH effectively reverses this upregulation. (* p < 0.05 versus control; #P < 0.05 versus LPS). (G) Representative micrographs show immunofluorescence staining of PLA2R in Beas-2B cells treated with LPS, with or without GSH. Scale bar = 100 μm. (H–M) Levels of oxidative stress, PLA2R expression, and EVs production in Beas-2B cells stimulated by RM8785, with or without GSH intervention. Representative Western blot (H) and quantitative data (I–M) are provided. The expression levels of NOX4 (I) , NOX2 (J) , PLA2R (K) , CD63 (L) , and TSG101 (M) are all significantly upregulated by RM8785, while GSH effectively reverses this upregulation. (* p < 0.05 versus control; #P < 0.05 versus PM2.5).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1516111/full'>39744137</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-noxa1-antibody-pa1930-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1930-noxa1-primary-antibodies-wb-testing-1.jpgAnti-NOXA1 Antibody Picoband&reg; Western blot analysis of NOXA1 using anti-NOXA1 antibody (PPA1930). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA1 antigen affinity purified polyclonal antibody (Catalog # PA1930) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOXA1 at approximately 60 kDa. The expected band size for NOXA1 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nucleophosmin-antibody-pa1931-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-wb-testing-1.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; Western blot analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: monkey COS-7 whole cell lysates, <br> Lane 5: human SKOV3 whole cell lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: human 22RV1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleophosmin antigen affinity purified polyclonal antibody (PA1931) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Nucleophosmin at approximately 38-40 kDa. The expected band size for Nucleophosmin is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-wb-testing-2.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; Western blot analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat C6 whole cell lysates, <br> Lane 2: rat NRK whole cell lysates, <br> Lane 3: rat PC-12 whole cell lysates, <br> Lane 4: rat RH35 whole cell lysates, <br> Lane 5: mouse ANA-1 whole cell lysates, <br> Lane 6: mouse RAW264.7 whole cell lysates, <br> Lane 7: mouse Neuro-2a whole cell lysates, <br> Lane 8: mouse HEPA1-6 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleophosmin antigen affinity purified polyclonal antibody (PA1931) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Nucleophosmin at approximately 38-40 kDa. The expected band size for Nucleophosmin is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-ihc-testing-3.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Nucleophosmin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nucleophosmin Antibody (PA1931) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-ihc-testing-4.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Nucleophosmin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nucleophosmin Antibody (PA1931) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-ihc-testing-5.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Nucleophosmin was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nucleophosmin Antibody (PA1931) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-ihc-testing-6.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Nucleophosmin was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nucleophosmin Antibody (PA1931) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-ihc-testing-7.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Nucleophosmin was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nucleophosmin Antibody (PA1931) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-ihc-testing-8.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Nucleophosmin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Nucleophosmin Antibody (PA1931) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-if-testing-9.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IF analysis of Nucleophosmin using anti-Nucleophosmin antibody (PA1931). <br> Nucleophosmin was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4 μg/mL rabbit anti-Nucleophosmin Antibody (PA1931) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1931-nucleophosmin-primary-antibodies-fcm-testing-10.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-Nucleophosmin antibody (PA1931). <br> Overlay histogram showing HL-60 cells stained with PA1931 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nucleophosmin Antibody (PA1931, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prohibitin-antibody-pa1932-boster.html2026-03-24T05:03:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1932-1-WB-anti-prohibitin-antibody.jpgAnti-Prohibitin/PHB Antibody Picoband&reg;Anti-Prohibitin antibody&#44; PA1932&#44; Western blotting<br>Lane 1: Rat Lung Tissue Lysate<br>Lane 2: Rat Skeletal Muscle Tissue Lysate<br>Lane 3: Rat Brain Tissue Lysate<br>Lane 4: Rat Kidney Tissue Lysate<br>Lane 5: HELA Cell Lysate<br>Lane 6: MCF-7 Cell Lysate<br>Lane 7: PC-12 Cell Lysate<br>Lane 8: A549 Cell Lysate<br>Lane 9: SMMC Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1932-2-IHC-anti-prohibitin-antibody.jpgAnti-Prohibitin/PHB Antibody Picoband&reg;Anti-Prohibitin antibody&#44; PA1932&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1932-3-IF-anti-prohibitin-antibody.jpgAnti-Prohibitin/PHB Antibody Picoband&reg;Anti-Prohibitin antibody&#44; PA1932&#44; ICC<br>ICC: HELA Cell<br><br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1932-4.jpgAnti-Prohibitin/PHB Antibody Picoband&reg; IHC analysis of Prohibitin using anti-Prohibitin antibody (PA1932). <br> Prohibitin was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Prohibitin Antibody (PA1932) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1932-5.jpgAnti-Prohibitin/PHB Antibody Picoband&reg; Western blot analysis of Prohibitin using anti-Prohibitin antibody (PA1932). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse NIH3T3 whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prohibitin antigen affinity purified polyclonal antibody (Catalog # PA1932) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prohibitin at approximately 30KD. The expected band size for Prohibitin is at 30KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1932-6.jpgAnti-Prohibitin/PHB Antibody Picoband&reg; IF analysis of Prohibitin using anti-Prohibitin antibody (PA1932). <br> Prohibitin was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Prohibitin Antibody (PA1932) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsc70-interacting-protein-hip-antibody-pa1935-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1935-st13-primary-antibodies-wb-testing-1.jpgAnti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband&reg; Western blot analysis of ST13 using anti-ST13 antibody (PA1935). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human SKOV3 whole cell lysates, <br> Lane 4: mouse testis tissue lysates, <br> Lane 5: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST13 antigen affinity purified polyclonal antibody (Catalog # PA1935) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST13 at approximately 45-54 kDa. The expected band size for ST13 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1935-st13-primary-antibodies-if-testing-2.jpgAnti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband&reg; IF analysis of ST13 using anti-ST13 antibody (PA1935). <br> ST13 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ST13 Antibody (PA1935) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mttfa-antibody-pa1936-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-wb-testing-1.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; Western blot analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human CACO-2 whole cell lysates,<br> Lane 4: huamn Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody (Catalog # PA1936) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24 kDa. The expected band size for mtTFA is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-ihc-testing-2.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-ihc-testing-3.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-ihc-testing-4.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-ihc-testing-5.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-ihc-testing-6.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-ihc-testing-7.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-ihc-testing-8.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1936-mttfa-primary-antibodies-if-testing-9.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IF analysis of mtTFA using anti-mtTFA antibody (PA1936). <br> mtTFA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-mtTFA Antibody (PA1936) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-4-antibody-pa1937-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1937-aqp4-primary-antibodies-wb-testing-1.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; Western blot analysis of AQP4 using anti-AQP4 antibody (PA1937). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP4 antigen affinity purified polyclonal antibody (Catalog # PA1937) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP4 at approximately 35 kDa. The expected band size for AQP4 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1937-aqp4-primary-antibodies-ihc-testing-2.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of AQP4 using anti-AQP4 antibody (PA1937). <br> AQP4 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP4 Antibody (PA1937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1937-aqp4-primary-antibodies-ihc-testing-3.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of AQP4 using anti-AQP4 antibody (PA1937). <br> AQP4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP4 Antibody (PA1937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1937-aqp4-primary-antibodies-ihc-testing-4.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of AQP4 using anti-AQP4 antibody (PA1937). <br> AQP4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP4 Antibody (PA1937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-asic3-antibody-pa1938-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1938-asic3-primary-antibodies-wb-testing-1_1.jpgAnti-Acid-sensing ion channel 3 ASIC3 Antibody Picoband&reg;Western blot analysis of ASIC3 using anti-ASIC3 antibody (PA1938). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human U87 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody (PA1938) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 59 kDa. The expected band size for ASIC3 is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cullin3-antibody-pa1939-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1939-cul3-primary-antibodies-wb-testing-1.jpgAnti-Cullin 3/CUL3 Antibody Picoband&reg; Western blot analysis of Cullin3 using anti-Cullin3 antibody (PA1939). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human Ramos whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: mouse brain tissue lysates, <br> Lane 6: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin3 antigen affinity purified polyclonal antibody (Catalog # PA1939) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin3 at approximately 89 kDa. The expected band size for Cullin3 is at 89 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gli2-antibody-pa1941-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1941-1-WB-anti-gli2-antibody.jpgAnti-Zinc finger protein GLI2 Gli2 Antibody Picoband&reg;Anti-Gli2 antibody&#44; PA1941&#44; Western blotting<br>WB: Human Placenta Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1941-2-WB-anti-gli2-antibody.jpgAnti-Zinc finger protein GLI2 Gli2 Antibody Picoband&reg;Anti-Gli2 antibody&#44; PA1941&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: SKOV Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br>Lane 5: A549 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irak4-antibody-pa1942-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1942-1-WB-anti-irak4-antibody.jpgAnti-IRAK4 Antibody Picoband&reg;Anti-IRAK4 antibody&#44; PA1942&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: U87 Cell Lysate<br>Lane 3: MCF-7 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd11b-antibody-pa1943-1-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1943-1-1-WB-anti-cd11b-antibody.jpgAnti-CD11b/ITGAM Antibody Picoband&reg;Anti-CD11b antibody&#44; PA1943-1&#44; All Western blotting<br>All lanes: Anti-ITGAM(PA1943-1) at 0.5ug/ml<br> Lane 1: JURKAT Whole Cell Lysate at 40ug<br> Lane 2: RAJI Whole Cell Lysate at 40ug <br>Predicted bind size: 127KD <br>Observed bind size: 170KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sapk4-antibody-pa1944-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1944-1-WB-anti-sapk4-antibody.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;Anti-SAPK4 antibody&#44; PA1944&#44; Western blotting<br>Lane 1: PANC Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-milk-fat-globule-1-antibody-pa1945-boster.html2026-04-01T05:01:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1945-mfge8-primary-antibodies-wb-testing-1.jpgAnti-Milk Fat Globule 1/MFGE8 Antibody Picoband&reg; Western blot analysis of MFGE8 using anti-MFGE8 antibody (PA1945). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human milk 5 μl,<br> Lane 2: human milk 2.5 μl.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MFGE8 antigen affinity purified polyclonal antibody (Catalog # PA1945) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MFGE8 at approximately 43 kDa. The expected band size for MFGE8 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1945-mfge8-primary-antibodies-ihc-testing-2.jpgAnti-Milk Fat Globule 1/MFGE8 Antibody Picoband&reg; IHC analysis of MFGE8 using anti-MFGE8 antibody (PA1945). <br> MFGE8 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MFGE8 Antibody (PA1945) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfkb-p100-p52-antibody-pa1946-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1946-1-IHC-anti-nfkb-p100-p52-antibody.jpgAnti-NFkB/NFKB2 p100/p52 Antibody Picoband&reg;Anti-NFkB p100/p52 antibody&#44; PA1946&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1946-2-WB-anti-nfkb-p100-p52-antibody.jpgAnti-NFkB/NFKB2 p100/p52 Antibody Picoband&reg;Anti-NFkB p100/p52 antibody&#44; PA1946&#44; Western blotting<br>Lane 1: Mouse Liver Tissue Lysate<br>Lane 2: HEPA Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nox5-antibody-pa1947-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1947-nox5-primary-antibodies-wb-testing-1.jpgAnti-NADPH oxidase 5 NOX5 Antibody Picoband&reg;Western blot analysis of NOX5 using anti-NOX5 antibody (PA1947). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX5 antigen affinity purified polyclonal antibody (PA1947) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOX5 at approximately 86 kDa. The expected band size for NOX5 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1947-nox5-primary-antibodies-ihc-testing-1.jpgAnti-NADPH oxidase 5 NOX5 Antibody Picoband&reg;IHC analysis of NOX5 using anti-NOX5 antibody (PA1947). <br>NOX5 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NOX5 Antibody (PA1947) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1947-nox5-primary-antibodies-if-testing-1.jpgAnti-NADPH oxidase 5 NOX5 Antibody Picoband&reg;IF analysis of NOX5 using anti-NOX5 antibody (PA1947). <br> NOX5 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NOX5 Antibody (PA1947) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nrf1-antibody-pa1948-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1948-2-IHC-anti-nrf1-antibody.jpgAnti-Nuclear respiratory factor 1 NRF1 Antibody Picoband&reg;Anti-NRF1 antibody&#44; PA1948&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1948-3-IF-anti-nrf1-antibody.jpgAnti-Nuclear respiratory factor 1 NRF1 Antibody Picoband&reg;Anti-NRF1 antibody&#44; PA1948&#44; ICC<br>ICC: A549 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1948-4-IHC-anti-nrf1-antibody.jpgAnti-Nuclear respiratory factor 1 NRF1 Antibody Picoband&reg;Anti-NRF1 antibody&#44; PA1948&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1948-5-IHC-anti-nrf1-antibody.jpgAnti-Nuclear respiratory factor 1 NRF1 Antibody Picoband&reg;Anti-NRF1 antibody&#44; PA1948&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1948-1.jpgAnti-Nuclear respiratory factor 1 NRF1 Antibody Picoband&reg;All lanes: Anti NRF1 (PA1948) at 0.5ug/ml<br>Lane 1: U2OS Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 75KD<br>Observed bind size: 75KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-osbp1-antibody-pa1949-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1949-1-WB-anti-osbp-osbp11-antibody.jpgAnti-OSBP1 Antibody Picoband&reg;Anti-OSBP1 antibody&#44; PA1949&#44; Western blotting<br>Lane 1: Rat Kidney Tissue Lysate<br>Lane 2: Rat Spleen Tissue Lysate<br>Lane 3: Rat Lung Tissue Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: A549 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd31-antibody-pa1950-1-boster.html2026-03-24T05:03:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-wb-testing-1_1.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (Catalog # PA1950-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 120 kDa. The expected band size for CD31/PECAM1 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-ihc-testing-2_1.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PA1950-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-ihc-testing-3_1.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PA1950-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-ihc-testing-4_1.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PA1950-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-ihc-testing-5.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PA1950-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-ihc-testing-6.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PA1950-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-if-testing-7.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IF analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD31/PECAM1 Antibody (PA1950-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-if-testing-8.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IF analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PA1950-1). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD31/PECAM1 Antibody (PA1950-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1950-1-pecam1-primary-antibodies-fcm-testing-9.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-CD31/PECAM1 antibody (PA1950-1). <br> Overlay histogram showing HEL cells stained with PA1950-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD31/PECAM1 Antibody (PA1950-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rea-antibody-pa1951-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1951-1-WB-anti-rea-antibody.jpgAnti-REA/PHB2 Antibody Picoband&reg;Anti-REA antibody&#44; PA1951&#44; Western blotting<br>Lane 1: PANC Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: U87Cell Lysate<br>Lane 4: HEPA Cell Lysate<br><br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1951-2-IHC-anti-rea-antibody.jpgAnti-REA/PHB2 Antibody Picoband&reg;Anti-REA antibody&#44; PA1951&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1951-3-IF-anti-rea-antibody.jpgAnti-REA/PHB2 Antibody Picoband&reg;Anti-REA antibody&#44; PA1951&#44; ICC<br>ICC: A549 Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-por-antibody-pa1952-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1952-por-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; Western blot analysis of POR using anti-POR antibody (PA1952). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody (Catalog # PA1952) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POR at approximately 77 kDa. The expected band size for POR is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1952-2-IHC-anti-por-cytochrome-p450-reductase-antibody.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg;Anti-POR antibody&#44; PA1952&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1952-3-IF-anti-por-cytochrome-p450-reductase-antibody.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg;Anti-POR antibody&#44; PA1952&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1952-4.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; IHC analysis of POR using anti-POR antibody (PA1952). <br> POR was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POR Antibody (PA1952) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1952-por-primary-antibodies-fcm-testing-4.pngAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-POR antibody (PA1952). <br> Overlay histogram showing A431 cells stained with PA1952 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POR Antibody (PA1952, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sqstm1-p62-antibody-pa1955-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-1_1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; Western blot analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human COLO-320 whole cell lysates, <br> Lane 3: human A549 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 antigen affinity purified polyclonal antibody (Catalog # PA1955) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQSTM1 at approximately 60KD. The expected band size for SQSTM1 is at 48KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-10.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-2_1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-3_1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-4_1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-5.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-6.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of human sarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-7.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of human cholangiocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-8.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-9.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-11.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-12.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IF analysis of SQSTM1 using anti-SQSTM1 antibody (PA1955). <br> SQSTM1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SQSTM1 Antibody (PA1955) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1955-13.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-SQSTM1 antibody (PA1955).<br> Overlay histogram showing A549 cells stained with PA1955 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SQSTM1 Antibody (PA1955,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tjp2-antibody-pa1957-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1957-tjp2-primary-antibodies-wb-testing-1.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; Western blot analysis of TJP2 using anti-TJP2 antibody (PA1957). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human Hacat whole cell lysates,<br> Lane 5: human MCF-7 whole cell lysates,<br> Lane 6: human U20S whole cell lysates,<br> Lane 7: human Hela whole cell lysates,<br> Lane 8: human PC-3 whole cell lysates,<br> Lane 9: rat liver tissue lysates,<br> Lane 10: rat RH35 whole cell lysates,<br> Lane 11: mouse liver tissue lysates,<br> Lane 12: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP2 antigen affinity purified polyclonal antibody (Catalog # PA1957) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP2 at approximately 150 kDa. The expected band size for TJP2 is at 134 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1957-2-IHC-anti-tjp2-zo-2-antibody.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; IHC analysis of TJP2 using anti-TJP2 antibody (PA1957). <br> TJP2 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TJP2 Antibody (PA1957) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1957-tjp2-primary-antibodies-if-testing-3.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; IF analysis of TJP2 using anti-TJP2 antibody (PA1957). <br> TJP2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-TJP2 Antibody (PA1957) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1957-tjp2-primary-antibodies-if-testing-4.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; IF analysis of TJP2 using anti-TJP2 antibody (PA1957). <br> TJP2 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-TJP2 Antibody (PA1957) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1957-tjp2-primary-antibodies-fcm-testing-5.pngAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-TJP2 antibody (PA1957). <br>Overlay histogram showing MCF-7 cells stained with PA1957 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TJP2 Antibody (PA1957, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr10-antibody-pa1958-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1958-1-WB-anti-tlr10-antibody.jpgAnti-Toll-like receptor 10 TLR10 Antibody Picoband&reg;Anti-TLR10 antibody&#44; PA1958&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: JURKAT Cell Lysate<br>Lane 3: CEMJ Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zeb2-antibody-pa1959-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1959-zeb2-primary-antibodies-wb-testing-1.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; Western blot analysis of ZEB2 using anti-ZEB2 antibody (PA1959). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZEB2 antigen affinity purified polyclonal antibody (Catalog # PA1959) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZEB2 at approximately 180 kDa. The expected band size for ZEB2 is at 136 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1959-40246_2023_480_fig10_html.pngAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg;LINC00945 promotes EMT, migration, and invasion of glioma cells. A , B Morphological changes were perceived in both LN18 ( A ) and PN12 ( B ) cells after culturing for three weeks following overexpression LINC00945. C – E The expression of LINC00945 was positively correlated with the expression of N-cadherin ( C ), ZEB1 ( D ), and ZEB2 ( E ) in the TCGA database. F , G The mRNA expression levels of EMT-related genes (N-cadherin, ZEB1, and ZEB2) in LN18 ( F ) and PN12 ( G ) glioma cells transfected with LINC00945 and vector plasmids were examined by qRT-PCR. H , I Western blot examined the different expression levels of N-cadherin, ZEB1, and ZEB2 proteins in the LINC00945 overexpression and vector groups. J , K Transwell experiments revealed that overexpression of LINC00945 promoted LN18 and PN12 glioma cell migration ( J ) and invasion ( K ). *** p < 0.001. The independent biological experiments were repeated at least three times <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40246-023-00480-w'>37004060</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1959-2.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; IHC analysis of ZEB2 using anti-ZEB2 antibody (PA1959). <br> ZEB2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ZEB2 Antibody (PA1959) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1959-3.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; IHC analysis of ZEB2 using anti-ZEB2 antibody (PA1959). <br> ZEB2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ZEB2 Antibody (PA1959) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1959-4.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; IHC analysis of ZEB2 using anti-ZEB2 antibody (PA1959). <br> ZEB2 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ZEB2 Antibody (PA1959) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-riam-antibody-pa1960-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1960_-apbb1ip-primary-antibodies-wb-testing-1.jpgAnti-RIAM/APBB1IP Antibody Picoband&reg; Western blot analysis of APBB1IP using anti-APBB1IP antibody (PA1960 ). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates&#44; <br> Lane 2: Jurkat whole cell lysates&#44; <br> Lane 3: human Raji whole cell lysates&#44; <br> Lane 4: U937 whole cell lysates&#44; <br> Lane 5: rat thymus tissue lysates&#44; <br> Lane 6: mouse thymus tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APBB1IP antigen affinity purified polyclonal antibody (Catalog # PA1960 ) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APBB1IP at approximately 110-120KD. The expected band size for APBB1IP is at 73KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1960-3-IF-anti-riam-antibody.jpgAnti-RIAM/APBB1IP Antibody Picoband&reg;Anti-RIAM antibody&#44; PA1960&#44; ICC<br>ICC: JURKAT Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1960-2-IHC-anti-riam-antibody.jpgAnti-RIAM/APBB1IP Antibody Picoband&reg;Anti-RIAM antibody&#44; PA1960&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1960_apbb1ip_ac1_rat_lymphaden_ihc-p.jpgAnti-RIAM/APBB1IP Antibody Picoband&reg; IHC analysis of APBB1IP using anti-APBB1IP antibody (PA1960). APBB1IP was detected in paraffin-embedded section of rat lymphaden. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APBB1IP Antibody (PA1960) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1960_apbb1ip_ac1_rat_spleen_ihc-f.jpgAnti-RIAM/APBB1IP Antibody Picoband&reg; IHC analysis of APBB1IP using anti-APBB1IP antibody (PA1960). APBB1IP was detected in frozen section of rat spleen. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with μg/ml rabbit anti-APBB1IP Antibody (PA1960) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1960-apbb1ip-primary-antibodies-if-testing-6.jpgAnti-RIAM/APBB1IP Antibody Picoband&reg; IF analysis of APBB1IP using anti-APBB1IP antibody (PA1960). <br> APBB1IP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-APBB1IP Antibody (PA1960) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1960-apbb1ip-primary-antibodies-fcm-testing-7.pngAnti-RIAM/APBB1IP Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-APBB1IP antibody (PA1960). <br>Overlay histogram showing SiHa cells stained with PA1960 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APBB1IP Antibody (PA1960, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-3-p17-antibody-pa1961-1-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1961-1-1-WB-anti-caspase-3-p17-antibody.jpgAnti-Caspase-3(P17)/CASP3 Antibody Picoband&reg; Western blot analysis of Caspase-3 (P17) using anti-Caspase-3 (P17) antibody (PA1961-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: Rat Heart Tissue Lysate<br>Lane 2: Rat Liver Tissue Lysate<br>Lane 3: Rat Thymus Tissue Lysate<br>Lane 4: Rat Spleen Tissue Lysate.<br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 (P17) antigen affinity purified polyclonal antibody (Catalog # PA1961-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 (P17) at approximately 32KD&#44;19KD. The expected band size for Caspase-3 (P17) is at 32KD&#44;17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1961-1-fneur-12-750908-t003.jpgAnti-Caspase-3(P17)/CASP3 Antibody Picoband&reg;Mean gray value analyzed according to western blotting results of IL-6, caspase-3, and cleaved-caspase-3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2021.750908/full'>34975719</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1961-1-fneur-12-750908-g001.jpgAnti-Caspase-3(P17)/CASP3 Antibody Picoband&reg;Experimental workflow. One hundred four rats were randomly divided into five groups: group S (sham, n = 20), group M (middle cerebral artery occlusion [MCAO], n = 28), group H2M (intermittent hypobaric hypoxia preconditioned MCAO group, 2 h/day, n = 20), group H6M (intermittent hypobaric hypoxia preconditioned MCAO group, 6 h/day, n = 28), and group HpM (persistent hypobaric hypoxia preconditioned MCAO group, n = 28). Behavioral tests and morphological staining (TTC staining) were used to analyze the severity of infarction. Total protein expression of NeuN (a specific marker of mature neurons), caspase-3, cleaved-caspase-3, and IL-6 was estimated using western blotting, which explained the severity of injury from different perspectives. Ultrastructural changes were observed under a transmission electron microscope. The most effective pretreatment group was selected for further label-free proteomic study and provided a reliable direction for mechanism exploration. Western blotting was used to verify the expression of the target protein, and key markers for the biological process were detected using immunofluorescence. caspase-3, cysteinyl aspartate specific proteinase 3; IL-6, interleukin 6; NeuN, neuron-specific nuclear protein; TTC, 2,3,5-triphenyl tetrazolium chloride.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2021.750908/full'>34975719</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1961-1-fneur-12-750908-g004.jpgAnti-Caspase-3(P17)/CASP3 Antibody Picoband&reg;Relative expression of IL-6, caspase-3, and cleaved-caspase-3. (A) Immunoblot results of IL-6, caspase-3, and cleaved-caspase-3. (B) Analysis of IL-6 relative expression. One-way ANOVA showed differences among the five groups, F = 10.86, p < 0.0001. (C) Analysis of caspase-3 relative expression. One-way ANOVA showed differences among the five groups, F = 8.50, p = 0.0004. (D) Analysis of cleaved-caspase-3 relative expression. One-way ANOVA showed differences among the five groups, F = 17.36, p < 0.0001. ANOVA, analysis of variance; caspase-3, cysteinyl aspartate specific proteinase 3; IL-6, interleukin 6.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2021.750908/full'>34975719</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1961-1-2-IHC-anti-caspase-3-p17-antibody.jpgAnti-Caspase-3(P17)/CASP3 Antibody Picoband&reg; IHC analysis of Caspase-3 (P17)using anti-Caspase-3 (P17)antibody (PA1961-1).<br> Caspase-3 (P17)was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caspase-3 (P17)Antibody (PA1961-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1961-1-3_1.jpgAnti-Caspase-3(P17)/CASP3 Antibody Picoband&reg; Western blot analysis of Caspase-3 (P17) using anti-Caspase-3 (P17) antibody (PA1961-1). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: mouse thymus tissue lysates&#44; <br>Lane 2: mouse spleen tissue lysates&#44; <br>Lane 3: mouse lung tissue lysates&#44; <br>Lane 4: mouse brain tissue lysates&#44;<br>Lane 5: mouse testis tissue lysates.<br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 (P17) antigen affinity purified polyclonal antibody (Catalog # PA1961-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 (P17) at approximately 32KD&#44;19KD. The expected band size for Caspase-3 (P17) is at 32KD&#44;17KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cuedc2-antibody-pa1962-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1962-1-WB-anti-cuedc2-antibody.jpgAnti-CUEDC2 Antibody Picoband&reg;Anti-CUEDC2 antibody&#44; PA1962&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant human CuEDC2&#44; 28.9KD (162aa tag+ N197-H287)<br>Lane 1: Recombinant Human CuEDC2 Protein 10ng <br>Lane 2: Recombinant Human CuEDC2 Protein 5ng <br>Lane 3: Recombinant Human CuEDC2 Protein 2.5ng <br>Lane 4: Recombinant Human CuEDC2 Protein 1.25nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1962-2-IHC-anti-cuedc2-antibody.jpgAnti-CUEDC2 Antibody Picoband&reg;Anti-CUEDC2 antibody&#44; PA1962&#44; IHC(P)<br>IHC(P): Human Thyroid Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddx4-mvh-antibody-pa1963-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1963-ddx4-primary-antibodies-wb-testing-1_1.jpgAnti-DDX4/MVH Antibody Picoband&reg; Western blot analysis of DDX4 using anti-DDX4 antibody (PA1963). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates,<br> Lane 2: mouse testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX4 antigen affinity purified polyclonal antibody (Catalog # PA1963) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX4 at approximately 76 kDa. The expected band size for DDX4 is at 76 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1963-3-IHC-anti-ddx4-mvh-antibody.jpgAnti-DDX4/MVH Antibody Picoband&reg;Anti-DDX4/MVH antibody&#44; PA1963&#44; IHC(P)<br>IHC(P): Rat Ovary Tissue<br><br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1963-4-IHC-anti-ddx4-mvh-antibody.jpgAnti-DDX4/MVH Antibody Picoband&reg;Anti-DDX4/MVH antibody&#44; PA1963&#44; IHC(P)<br>IHC(P): Rat Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1963-5-IF-anti-ddx4-mvh-antibody.jpgAnti-DDX4/MVH Antibody Picoband&reg;Anti-DDX4/MVH antibody&#44; PA1963&#44; ICC<br>ICC: MCF-7 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1963-6-IHC-anti-ddx4-mvh-antibody.jpgAnti-DDX4/MVH Antibody Picoband&reg;Anti-DDX4/MVH antibody&#44; PA1963&#44; IHC(F)<br>IHC(F): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1963-2_1.jpgAnti-DDX4/MVH Antibody Picoband&reg; IHC analysis of DDX4/MVH using anti-DDX4/MVH antibody (PA1963). <br> DDX4/MVH was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDX4/MVH Antibody (PA1963) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1963-ddx4-primary-antibodies-if-testing-7.jpgAnti-DDX4/MVH Antibody Picoband&reg; IF analysis of DDX4/MVH using anti- DDX4/MVH antibody (PA1963). <br> DDX4/MVH was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- DDX4/MVH Antibody (PA1963) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1963-8.pngAnti-DDX4/MVH Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-DDX4/MVH antibody (PA1963). <br> Overlay histogram showing PC-3 cells stained with PA1963 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (PA1963&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1963-9.pngAnti-DDX4/MVH Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-DDX4/MVH antibody (PA1963). <br> Overlay histogram showing SiHa cells stained with PA1963 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (PA1963&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddx5-antibody-pa1964-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1964-ddx5-primary-antibodies-wb-testing-1.jpgAnti-DDX5 Antibody Picoband&reg; Western blot analysis of DDX5 using anti-DDX5 antibody (PA1964). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX5 antigen affinity purified polyclonal antibody (Catalog # PA1964) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX5 at approximately 69 kDa. The expected band size for DDX5 is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1964-ddx5-primary-antibodies-ihc-testing-3.jpgAnti-DDX5 Antibody Picoband&reg;IHC analysis of p68/DDX5 using anti-p68/DDX5 antibody (PA1964). <br>p68/DDX5 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p68/DDX5 Antibody (PA1964) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1964-2-IHC-anti-ddx5-antibody.jpgAnti-DDX5 Antibody Picoband&reg; IHC analysis of DDX5 using anti-DDX5 antibody (PA1964). <br> DDX5 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DDX5 Antibody (PA1964) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1964-3.pngAnti-DDX5 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-DDX5 antibody (PA1964). <br> Overlay histogram showing A431 cells stained with PA1964 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX5 Antibody (PA1964&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1964-4.jpgAnti-DDX5 Antibody Picoband&reg; IF analysis of DDX5 using anti-DDX5 antibody (PA1964). <br> DDX5 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDX5 Antibody (PA1964) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegfr1-flt1-antibody-pa1966-1-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1966-1-flt1-primary-antibodies-wb-testing-1.jpgAnti-VEGF Receptor 1/FLT1 Antibody Picoband&reg; Western blot analysis of FLT1 using anti-FLT1 antibody (PA1966-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat C6 whole cell lysates,<br> Lane 3: mosue brain tissue lysates,<br> Lane 4: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLT1 antigen affinity purified polyclonal antibody (Catalog # PA1966-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLT1 at approximately 200 kDa. The expected band size for FLT1 is at 150 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lcat-antibody-pa1967-boster.html2026-03-24T05:03:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1967-1-WB-anti-lcat-antibody.jpgAnti-LCAT Antibody Picoband&reg;Anti-LCAT antibody&#44; PA1967&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: U87 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: SMMC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ltbr-antibody-pa1968-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1968-1-IHC-anti-ltbr-tnfrsf3-antibody.jpgAnti-LTBR Antibody Picoband&reg;Anti-LTBR antibody&#44; PA1968&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1968-2-WB-anti-ltbr-tnfrsf3-antibody.jpgAnti-LTBR Antibody Picoband&reg;Anti-LTBR antibody&#44; PA1968&#44; Western blotting<br>All lanes: Anti LTBR (PA1968) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Predicted bind size: 47KD<br>Observed bind size: 60KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nrg1-antibody-pa1969-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1969-nrg1-primary-antibodies-wb-testing-1.jpgAnti-NRG1 Antibody Picoband&reg; Western blot analysis of NRG1 using anti-NRG1 antibody (PA1969). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: huamn A549 whole cell lysates,<br> Lane 2: human Hacat whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRG1 antigen affinity purified polyclonal antibody (Catalog # PA1969) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRG1 at approximately 40, 65 and 100 kDa. The expected band size for NRG1 is at 25, 40, 65 and 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1969-nrg1-primary-antibodies-ihc-testing-2.jpgAnti-NRG1 Antibody Picoband&reg; IHC analysis of NRG1 using anti-NRG1 antibody (PA1969). <br> NRG1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NRG1 Antibody (PA1969) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1969-nrg1-primary-antibodies-if-testing-3.jpgAnti-NRG1 Antibody Picoband&reg; IF analysis of NRG1 using anti-NRG1 antibody (PA1969) and anti-Beta Tubulin antibody (M01857-3).<br> NRG1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NRG1 Antibody (PA1969) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dna-pkcs-antibody-pa1970-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1970-prkdc-primary-antibodies-wb-testing-1.jpgAnti-DNA PKcs/PRKDC Antibody Picoband&reg; Western blot analysis of PRKDC using anti-PRKDC antibody (PA1970). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HEK293 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKDC antigen affinity purified polyclonal antibody (Catalog # PA1970) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRKDC at approximately 460 kDa. The expected band size for PRKDC is at 469 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tjp2-antibody-pa1971-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1971-tjp2-primary-antibodies-wb-testing-1_1.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg;Western blot analysis of TJP2 using anti-TJP2 antibody (PA1971). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: human U2OS whole cell lysates,<br> Lane 6: human Hela whole cell lysates,<br> Lane 7: human PC-3 whole cell lysates,<br> Lane 8: rat RH35 whole cell lysates,<br> Lane 9: rat HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP2 antigen affinity purified polyclonal antibody (Catalog # PA1971) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP2 at approximately 150 kDa. The expected band size for TJP2 is at 131 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1971-tjp2-primary-antibodies-ihc-testing-4.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg;IHC analysis of ZO-2/TJP2 using anti-ZO-2/TJP2 antibody (PA1971). <br>ZO-2/TJP2 was detected in a paraffin-embedded section of human stoamch tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZO-2/TJP2 Antibody (PA1971) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1971-tjp2-primary-antibodies-ihc-testing-5.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg;IHC analysis of ZO-2/TJP2 using anti-ZO-2/TJP2 antibody (PA1971). <br>ZO-2/TJP2 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZO-2/TJP2 Antibody (PA1971) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1971-tjp2-primary-antibodies-ihc-testing-1.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg;IHC analysis of TJP2 using anti-TJP2 antibody (PA1971). <br> TJP2 was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TJP2 Antibody (PA1971) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1971-tjp2-primary-antibodies-ihc-testing-2.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; IHC analysis of TJP2 using anti-TJP2 antibody (PA1971). <br> TJP2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TJP2 Antibody (PA1971) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1971-tjp2-primary-antibodies-ihc-testing-3.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; IHC analysis of TJP2 using anti-TJP2 antibody (PA1971). <br> TJP2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TJP2 Antibody (PA1971) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1971-tjp2-primary-antibodies-if-testing-4.jpgAnti-Tight junction protein ZO-2 TJP2 Antibody Picoband&reg; IF analysis of TJP2 using anti-TJP2 antibody (PA1971). <br> TJP2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TJP2 Antibody (PA1971) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zonula-occludens-protein-3-antibody-pa1972-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1972-tjp3-primary-antibodies-wb-testing-1.jpgAnti-Zonula occludens protein 3/TJP3 Antibody Picoband&reg;Western blot analysis of TJP3 using anti-TJP3 antibody (PA1972). <br>Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: human Caco-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human T-47D whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP3 antigen affinity purified polyclonal antibody (PA1972) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TJP3 at approximately 130-140 kDa. The expected band size for TJP3 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1972-tjp3-primary-antibodies-ihc-testing-1.jpgAnti-Zonula occludens protein 3/TJP3 Antibody Picoband&reg;IHC analysis of TJP3 using anti-TJP3 antibody (PA1972). <br>TJP3 was detected in a paraffin-embedded section of human fallopian tube tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TJP3 Antibody (PA1972) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1972-tjp3-primary-antibodies-if-testing-1.jpgAnti-Zonula occludens protein 3/TJP3 Antibody Picoband&reg;IF analysis of TJP3 using anti-TJP3 antibody (PA1972). <br> TJP3 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TJP3 Antibody (PA1972) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1972-tjp3-primary-antibodies-fcm-testing-1.jpgAnti-Zonula occludens protein 3/TJP3 Antibody Picoband&reg;Flow Cytometry analysis of CACO-2 cells using anti-TJP3 antibody (PA1972). <br>Overlay histogram showing CACO-2 cells stained with PA1972 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TJP3 Antibody (PA1972, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zonula-occludens-protein-3-antibody-pa1973-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1973-1-WB-anti-zonula-occludens-protein-3-antibody.jpgAnti-Zonula occludens protein 3/TJP3 Antibody Picoband&reg;Anti-Zonula occludens protein 3 antibody&#44; PA1973&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Heart Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1973-2-IHC-anti-zonula-occludens-protein-3-antibody.jpgAnti-Zonula occludens protein 3/TJP3 Antibody Picoband&reg;Anti-Zonula occludens protein 3 antibody&#44; PA1973&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1973-3-IHC-anti-zonula-occludens-protein-3-antibody.jpgAnti-Zonula occludens protein 3/TJP3 Antibody Picoband&reg;Anti-Zonula occludens protein 3 antibody&#44; PA1973&#44; IHC(P)<br>IHC(P): Mouse Liver Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-receptor-ii-antibody-pa1974-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1974-1-WB-anti-stnfsr-ii-antibody.jpgAnti-TNF Receptor II/TNFRSF1B Antibody Picoband&reg;Anti-TNF Receptor II antibody&#44; PA1974&#44; Western blotting<br>Lane 1: Rat Spleen Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: Mouse Brain Tissue Lysate<br>Lane 4: HEPA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dr4-antibody-pa1975-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1975-1-WB-anti-dr4-antibody.jpgAnti-DR4/TNFRSF10A Antibody Picoband&reg;Anti-DR4 antibody&#44; PA1975&#44; Western blotting<br>Lane 1: SW620 Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-53bp1-antibody-pa1976-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1976-tp53bp1-primary-antibodies-wb-testing-1.jpgAnti-53BP1/TP53BP1 Antibody Picoband&reg; Western blot analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human RT4 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53BP1 antigen affinity purified polyclonal antibody (Catalog # PA1976) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TP53BP1 at approximately 450 kDa. The expected band size for TP53BP1 is at 214 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1976-2-IHC-anti-53bp1-antibody.jpgAnti-53BP1/TP53BP1 Antibody Picoband&reg; IHC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976). <br> TP53BP1 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1976-3-IHC-anti-53bp1-antibody.jpgAnti-53BP1/TP53BP1 Antibody Picoband&reg; IHC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976). <br> TP53BP1 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1976-4-IHC-anti-53bp1-antibody.jpgAnti-53BP1/TP53BP1 Antibody Picoband&reg; IHC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976). <br> TP53BP1 was detected in a paraffin-embedded section of Mouse Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1976-5-IF-anti-53bp1-antibody.jpgAnti-53BP1/TP53BP1 Antibody Picoband&reg; ICC analysis of TP53BP1 using anti-TP53BP1 antibody (PA1976). <br> TP53BP1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-TP53BP1 Antibody (PA1976) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vrl1-antibody-pa1977-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1977-1-IHC-anti-vrl1-antibody.jpgAnti-VRL1/TRPV2 Antibody Picoband&reg;Anti-VRL1 antibody&#44; PA1977&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1977-2-IHC-anti-vrl1-antibody.jpgAnti-VRL1/TRPV2 Antibody Picoband&reg;Anti-VRL1 antibody&#44; PA1977&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1977-3-WB-anti-vrl1-antibody.jpgAnti-VRL1/TRPV2 Antibody Picoband&reg;Anti-VRL1 antibody&#44; PA1977&#44; Western blotting<br>WB: HELA Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpv3-antibody-pa1978-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1978-1-WB-anti-trpv3-antibody.jpgAnti-TRPV3 Antibody Picoband&reg;Anti-TRPV3 antibody&#44; PA1978&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: A549 Cell Lysate<br>Lane 3: MCF-7 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vdr-antibody-pa1983-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1983-1-WB-anti-vdr-nr1i1-antibody.jpgAnti-Vitamin D Receptor/VDR Antibody Picoband&reg;Anti-VDR antibody&#44; PA1983&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: HELA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vgf-antibody-pa1984-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1984-1-WB-anti-vgf-antibody.jpgAnti-Neurosecretory protein VGF VGF Antibody Picoband&reg;Anti-VGF antibody&#44; PA1984&#44; Western blotting<br>Lane 1: Rat Brain Tissue<br>Lane 2: U87 Cell Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: SHG Cell Lysate<br>Lane 5: NEURO Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt2b-antibody-pa1985-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1985-1-WB-anti-wnt2b-antibody.jpgAnti-Protein Wnt-2b Wnt2b Antibody Picoband&reg;Anti-Wnt2b antibody&#44; PA1985&#44; Western blotting<br>Recombinant Protein Detection Source :E.coli derived -recombinant Human WNT2B&#44; 35.78KD (162aa tag+ R101-A254) <br>Lane 1: Recombinant Human WNT2B Protein 10ng <br>Lane 2: Recombinant Human WNT2B Protein 5ng <br>Lane 3: Recombinant Human WNT2B Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-torc1-antibody-pa1987-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1987-1_1.jpgAnti-TORC1/CRTC1 Antibody Picoband&reg; Western blot analysis of TORC1 using anti-TORC1 antibody (PA1987).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC1 antigen affinity purified polyclonal antibody (Catalog # PA1987) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC1 at approximately 78KD. The expected band size for TORC1 is at 67KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-torc2-antibody-pa1988-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1988-crtc2-primary-antibodies-wb-testing-1.jpgAnti-TORC2/CRTC2 Antibody Picoband&reg; Western blot analysis of CRTC2 using anti-CRTC2 antibody (PA1988). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human MOLT-4 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRTC2 antigen affinity purified polyclonal antibody (Catalog # PA1988) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRTC2 at approximately 80 kDa. The expected band size for CRTC2 is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegf-receptor-2-antibody-pa1989-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1989-1-WB-anti-vegfr2-kdr-antibody.jpgAnti-VEGF Receptor 2/KDR Antibody Picoband&reg;Anti-VEGF Receptor 2 antibody&#44; PA1989&#44; Western blotting<br>WB: SMMC Cell Lysate<br><br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1989-2-IHC-anti-vegfr2-kdr-antibody.jpgAnti-VEGF Receptor 2/KDR Antibody Picoband&reg;Anti-VEGF Receptor 2 antibody&#44; PA1989&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1989-3-IF-anti-vegfr2-kdr-antibody.jpgAnti-VEGF Receptor 2/KDR Antibody Picoband&reg;Anti-VEGF Receptor 2 antibody&#44; PA1989&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1989-4-IHC-anti-vegfr2-kdr-antibody.jpgAnti-VEGF Receptor 2/KDR Antibody Picoband&reg;Anti-VEGF Receptor 2 antibody&#44; PA1989&#44; IHC(F)<br>IHC(F): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ltk-antibody-pa1990-boster.html2026-03-24T05:03:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1990-1_1.jpgAnti-LTK Antibody Picoband&reg; Western blot analysis of LTK using anti-LTK antibody (PA1990). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates<br> Lane 2: human Caco-2 whole cell lysates<br> Lane 3: human K562 whole cell lysates<br> Lane 4: human Jurkat whole cell lysates<br> Lane 5: human MDA-MB-453 whole cell lysates<br> Lane 6: human U2OS whole cell lysates<br> Lane 7: human PC-3 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTK antigen affinity purified polyclonal antibody (Catalog # PA1990) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTK at approximately 85-91KD. The expected band size for LTK is at 91KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1990-2.jpgAnti-LTK Antibody Picoband&reg; Western blot analysis of LTK using anti-LTK antibody (PA1990). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates<br> Lane 2: rat brain tissue lysates<br> Lane 3: mouse brain tissue lysates<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LTK antigen affinity purified polyclonal antibody (Catalog # PA1990) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LTK at approximately 85-91KD. The expected band size for LTK is at 91KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-npm2-antibody-pa1991-boster.html2026-03-25T05:21:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1991-1-WB-anti-npm2-nucleoplasmin-2-antibody.jpgAnti-Nucleoplasmin-2 NPM2 Antibody Picoband&reg;Anti-NPM2 antibody&#44; PA1991&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: U87 Cell Lysate<br>Lane 3: A549 Cell Lysate<br>Lane 4: SMMC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trkc-antibody-pa1992-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1992-1-WB-anti-trkc-antibody.jpgAnti-TrkC/NTRK3 Antibody Picoband&reg;Anti-TrkC antibody&#44; PA1992&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br>Lane 3: U87 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trkc-antibody-pa1992-1-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1992-1-1-WB-anti-trkc-antibody.jpgAnti-TrkC/NTRK3 Antibody Picoband&reg;Anti-TrkC antibody&#44; PA1992-1&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br>Lane 3: U87 Cell Lysate<br>Lane 4: SHG Cell Lysate<br>Lane 5: NEURO Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1992-1-2-IHC-anti-trkc-antibody.jpgAnti-TrkC/NTRK3 Antibody Picoband&reg;Anti-TrkC antibody&#44; PA1992-1&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1992-1-3-IHC-anti-trkc-antibody.jpgAnti-TrkC/NTRK3 Antibody Picoband&reg;Anti-TrkC antibody&#44; PA1992-1&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mitochondrial-pyruvate-dehydrogenase-kinase-1-antibody-pa1993-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1993-1-WB-anti-mitochondrial-pyruvate-dehydrogenase-kinase-1-antibody.jpgAnti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband&reg;Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody&#44; PA1993&#44; Western blotting<br>Lane 1: Rat Heart Tissue Lysate<br>Lane 2: Rat Skeletal Muscle Tissue Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: M231 Cell Lysate<br>Lane 5: COLO320 Cell Lysate<br>Lane 6: SW620 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1993-2-IHC-anti-mitochondrial-pyruvate-dehydrogenase-kinase-1-antibody.jpgAnti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody Picoband&reg;Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody&#44; PA1993&#44; IHC(P)<br>IHC(P): Human Prostatic Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdk2-antibody-pa1994-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1994-pdk2-primary-antibodies-wb-testing-1.jpgAnti-PDK2 Antibody Picoband&reg;Western blot analysis of PDK2 using anti-PDK2 antibody (PA1994). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates, <br> Lane 2: rat skeletal muscle tissue lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: rat kidney tissue lysates, <br> Lane 5: mouse heart tissue lysates, <br> Lane 6: mouse skeletal muscle tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK2 antigen affinity purified polyclonal antibody (PA1994) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDK2 at approximately 46 kDa. The expected band size for PDK2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1994-pdk2-primary-antibodies-if-testing-1.jpgAnti-PDK2 Antibody Picoband&reg;IF analysis of PDK2 using anti-PDK2 antibody (PA1994). <br> PDK2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PDK2 Antibody (PA1994) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1994-pdk2-primary-antibodies-ip-testing-1.jpgAnti-PDK2 Antibody Picoband&reg;Immunoprecipitating (IP) PDK2 in Jurkat whole cell lysate.<br> Western blot analysis of PDK2 using anti-PDK2 antibody (PA1994); <br> Lane 1: Jurkat whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-PDK2 antibody in Jurkat whole cell lysate;<br> Lane 3: anti-PDK2 antibody (2μg) + Jurkat whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PDK2 antigen affinity purified polyclonal antibody (PA1994) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PDK2 at approximately 46 kDa. The expected band size for PDK2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1994-pdk2-primary-antibodies-fcm-testing-1.jpgAnti-PDK2 Antibody Picoband&reg;Flow Cytometry analysis of PC-3 cells using anti-PDK2 antibody (PA1994). <br> Overlay histogram showing PC-3 cells stained with PA1994 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDK2 Antibody (PA1994, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-scly-antibody-pa1995-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1995-1-WB-anti-scly-selenocysteine-lyase-antibody.jpgAnti-Selenocysteine lyase SCLY Antibody Picoband&reg;Anti-SCLY antibody&#44; PA1995&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: 293T Cell Lysate<br>Lane 4: MCF-7 Cell Lysate<br>Lane 5: COLO320 Cell Lysate<br>Lane 6: HE1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-t-bet-tbx21-antibody-pa1999-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1999-1_1.jpgAnti-T-bet/Tbx21 Antibody Picoband&reg; Western blot analysis of T-bet using anti-T-bet antibody (PA1999). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse thymus tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-T-bet antigen affinity purified polyclonal antibody (Catalog # PA1999) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for T-bet at approximately 58-60KD. The expected band size for T-bet is at 58KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tec-antibody-pa2000-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2000-tec-primary-antibodies-wb-testing-1_1.jpgAnti-Tyrosine-protein kinase Tec Tec Antibody Picoband&reg;Western blot analysis of Tec using anti-Tec antibody (PA2000). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tec antigen affinity purified polyclonal antibody (Catalog # PA2000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Tec at approximately 74 kDa. The expected band size for Tec is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2000-tec-primary-antibodies-wb-testing-2.jpgAnti-Tyrosine-protein kinase Tec Tec Antibody Picoband&reg;Western blot analysis of Tec using anti-Tec antibody (PA2000). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates,<br> Lane 2: rat thymus tissue lysates,<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tec antigen affinity purified polyclonal antibody (Catalog # PA2000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Tec at approximately 74 kDa. The expected band size for Tec is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2000-2-ihc-anti-tec-antibody.jpgAnti-Tyrosine-protein kinase Tec Tec Antibody Picoband&reg; IHC analysis of Tec using anti-Tec antibody (PA2000). <br> Tec was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Tec Antibody (PA2000) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2000-3-ihc-anti-tec-antibody.jpgAnti-Tyrosine-protein kinase Tec Tec Antibody Picoband&reg; IHC analysis of Tec using anti-Tec antibody (PA2000). <br> Tec was detected in a paraffin-embedded section of Rat Liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Tec Antibody (PA2000) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dr3-antibody-pa2004-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2004-1-WB-anti-tnfrsf25-dr3-antibody.jpgAnti-DR3/TNFRSF25 Antibody Picoband&reg;Anti-DR3 antibody&#44; PA2004&#44; Western blotting<br>All lanes: Anti DR3 (PA2004) at 0.5ug/ml<br>WB: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 59KD<br>Observed bind size: 59KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tollip-antibody-pa2005-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-wb-testing-1.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg; Western blot analysis of Tollip using anti-Tollip antibody (PA2005). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human U2OS whole cell lysates,<br> Lane 3: human SW620 whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tollip antigen affinity purified polyclonal antibody (Catalog # PA2005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tollip at approximately 30 kDa. The expected band size for Tollip is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-ihc-testing-2.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg; IHC analysis of Tollip using anti-Tollip antibody (PA2005). <br> Tollip was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tollip Antibody (PA2005) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-ip-testing-1.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg;Immunoprecipitating (IP) Tollip in Hela whole cell lysate.<br> Western blot analysis of Tollip using anti-Tollip antibody (PA2005); <br> Lane 1: Hela whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Tollip antibody in Hela whole cell lysate;<br> Lane 3: anti-Tollip antibody (2μg) + Hela whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Tollip antigen affinity purified polyclonal antibody (PA2005) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Tollip at approximately 30 kDa. The expected band size for Tollip is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-ihc-testing-3.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg; IHC analysis of Tollip using anti-Tollip antibody (PA2005). <br> Tollip was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tollip Antibody (PA2005) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-fcm-testing-7.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg; Flow Cytometry analysis of Daudi cells using anti-Tollip antibody (PA2005). <br>Overlay histogram showing Daudi cells stained with PA2005 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tollip Antibody (PA2005, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-ihc-testing-4.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg; IHC analysis of Tollip using anti-Tollip antibody (PA2005). <br> Tollip was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tollip Antibody (PA2005) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-ihc-testing-5.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg; IHC analysis of Tollip using anti-Tollip antibody (PA2005). <br> Tollip was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tollip Antibody (PA2005) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2005-tollip-primary-antibodies-if-testing-6.jpgAnti-Toll-interacting protein Tollip Antibody Picoband&reg; IF analysis of Tollip using anti-Tollip antibody (PA2005). <br> Tollip was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tollip Antibody (PA2005) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-traf1-antibody-pa2006-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2006-1_1.jpgAnti-TRAF1 Antibody Picoband&reg; Western blot analysis of TRAF1 using anti-TRAF1 antibody (PA2006). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates&#44; <br> Lane 2: human T-47D whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF1 antigen affinity purified polyclonal antibody (Catalog # PA2006) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF1 at approximately 46KD. The expected band size for TRAF1 is at 46KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-traf2-antibody-pa2007-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2007-2-IHC-anti-traf2-antibody.jpgAnti-TRAF2 Antibody Picoband&reg;Anti-TRAF2 antibody&#44; PA2007&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2007-3_1-IF-anti-traf2-antibody.jpgAnti-TRAF2 Antibody Picoband&reg;Anti-TRAF2 antibody&#44; PA2007&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2007-1_1.jpgAnti-TRAF2 Antibody Picoband&reg; Western blot analysis of TRAF2 using anti-TRAF2 antibody (PA2007). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysate&#44; <br> Lane 2: rat PC-12 whole cell lysate&#44; <br> Lane 3: mouse spleen tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF2 antigen affinity purified polyclonal antibody (Catalog # PA2007) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF2 at approximately 56KD. The expected band size for TRAF2 is at 56KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-traf3-antibody-pa2008-boster.html2026-03-24T05:03:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2008-1-WB-anti-traf3-antibody.jpgAnti-TRAF3 Antibody Picoband&reg;Anti-TRAF3 antibody&#44; PA2008&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human TRAF3&#44; 30.6KD (162aa tag+ R341-Y449)<br>Lane 1: Recombinant Human TRAF3 Protein 5ng<br>Lane 2: Recombinant Human TRAF3 Protein 2.5ng <br>Lane 3: Recombinant Human TRAF3 Protein 1.25ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-traf4-antibody-pa2009-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2009-1-WB-anti-traf4-antibody.jpgAnti-TRAF4 Antibody Picoband&reg;Anti-TRAF4 antibody&#44; PA2009&#44; Western blotting<br>Lane 1: Rat Thymus Tissue Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br>Lane 4: HEPA Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2009-2-IHC-anti-traf4-antibody.jpgAnti-TRAF4 Antibody Picoband&reg;Anti-TRAF4 antibody&#44; PA2009&#44; IHC(P)<br>IHC(P): Rat Lymphonodus Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2009-3-IHC-anti-traf4-antibody.jpgAnti-TRAF4 Antibody Picoband&reg;Anti-TRAF4 antibody&#44; PA2009&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tram1-antibody-pa2010-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2010-2-IHC-anti-tram1-antibody.jpgAnti-TRAM1 Antibody Picoband&reg;Anti-TRAM1 antibody&#44; PA2010&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2010-3-IHC-anti-tram1-antibody.jpgAnti-TRAM1 Antibody Picoband&reg;Anti-TRAM1 antibody&#44; PA2010&#44; IHC(P)<br>IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2010-4-IF-anti-tram1-antibody.jpgAnti-TRAM1 Antibody Picoband&reg;Anti-TRAM1 antibody&#44; PA2010&#44; ICC<br>ICC: HELA Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2010-1_1.jpgAnti-TRAM1 Antibody Picoband&reg;Anti-TRAM1 antibody&#44; PA2010&#44;WB&#44;<br>Lane 1: Rat Brain Tissue Lysate<br> Lane 2: Rat Kidney Tissue Lysate<br> Lane 3: 293T Cell Lysate<br> Lane 4: RAJI Cell Lysate<br> Lane 5: JURKAT Cell Lysate<br> Lane 6: Hela Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2010-5.jpgAnti-TRAM1 Antibody Picoband&reg; IHC analysis of TRAM1 using anti-TRAM1 antibody (PA2010). <br> TRAM1 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAM1 Antibody (PA2010) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2010-6.jpgAnti-TRAM1 Antibody Picoband&reg; IHC analysis of TRAM1 using anti-TRAM1 antibody (PA2010). <br> TRAM1 was detected in frozen section of rat brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TRAM1 Antibody (PA2010) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tram2-antibody-pa2011-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2011-1-WB-anti-tram2-antibody.jpgAnti-TRAM2 Antibody Picoband&reg;Anti-TRAM2 antibody&#44; PA2011&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Mouse TRAM2&#44;39.0KD (162aa tag+R190-P370)<br>Lane 1: Recombinant Mouse TRAM2 Protein 10ng <br>Lane 2: Recombinant Mouse TRAM2 Protein 5ng <br>Lane 3: Recombinant Mouse TRAM2 Protein 2.5ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hamartin-antibody-pa2012-boster.html2026-04-01T05:01:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2012-1-WB-anti-hamartin-antibody.jpgAnti-Hamartin/TSC1 Antibody Picoband&reg;Anti-Hamartin antibody&#44; PA2012&#44; Western blotting<br>All lanes: Anti Hamartin (PA2012) at 0.5ug/ml<br>Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Lane 2: Rat Heart Tissue Lysate at 50ug<br>Lane 3: Rat Brain Tissue Lysate at 50ug<br>Lane 4: Rat Lung Tissue Lysate at 50ug<br>Lane 5: 293T Whole Cell Lysate at 40ug<br>Lane 6: HELA Whole Cell Lysate at 40ug<br>Lane 7: HT1080 Whole Cell Lysate at 40ug<br>Lane 8: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 130KD<br>Observed bind size: 160KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2012-2-IHC-anti-hamartin-antibody.jpgAnti-Hamartin/TSC1 Antibody Picoband&reg;Anti-Hamartin antibody&#44; PA2012&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2012-3-IHC-anti-hamartin-antibody.jpgAnti-Hamartin/TSC1 Antibody Picoband&reg;Anti-Hamartin antibody&#44; PA2012&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adiponectin-antibody-pa2014-1-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2014-1-1-WB-anti-adiponectin-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg;Anti-Adiponectin antibody&#44; PA2014-1&#44; Western blotting<br>WB: Rat Heart Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2014-1-2-WB-anti-adiponectin-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg;Anti-Adiponectin antibody&#44; PA2014-1&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human ADIPOQ&#44; 44.4 KD (162aa tag+ M1-N244)<br>Lane 1: Recombinant Human ADIPOQ Protein 10ng <br>Lane 2: Recombinant Human ADIPOQ Protein 5ng <br>Lane 3: Recombinant Human ADIPOQ Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2014-1-3-IHC-anti-adiponectin-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg;Anti-Adiponectin antibody&#44; PA2014-1&#44; IHC(P)<br>IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2014-1-4-IHC-anti-adiponectin-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg;Anti-Adiponectin antibody&#44; PA2014-1&#44; IHC(P)<br>IHC(P): Mouse Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2014-1-5-IHC-anti-adiponectin-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg;Anti-Adiponectin antibody&#44; PA2014-1&#44; IHC(F)<br>IHC(F): Rat Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2014-1-6-IHC-anti-adiponectin-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg;Anti-Adiponectin antibody&#44; PA2014-1&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bid-antibody-pa2015-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2015-bid-primary-antibodies-wb-testing-1.jpgAnti-Bid Antibody Picoband&reg; Western blot analysis of Bid using anti-Bid antibody (PA2015). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse L929 whole cell lysates, <br> Lane 2: mouse spleen tissue lysates, <br> Lane 3: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bid antigen affinity purified polyclonal antibody (Catalog # PA2015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bid at approximately 22 kDa. The expected band size for Bid is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2015-bid-primary-antibodies-ihc-testing-2.jpgAnti-Bid Antibody Picoband&reg; IHC analysis of Bid using anti-Bid antibody (PA2015). <br> Bid was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bid Antibody (PA2015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2015-bid-primary-antibodies-fcm-testing-4.pngAnti-Bid Antibody Picoband&reg; Flow Cytometry analysis of Neuro-2a cells using anti-Bid antibody (PA2015). <br>Overlay histogram showing Neuro-2a cells stained with PA2015 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bid Antibody (PA2015, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2015-bid-primary-antibodies-ihc-testing-3.jpgAnti-Bid Antibody Picoband&reg; IHC analysis of Bid using anti-Bid antibody (PA2015). <br> Bid was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bid Antibody (PA2015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmpr1b-antibody-pa2017-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2017-1-WB-anti-bmpr1b-alk-6-antibody.jpgAnti-BMPR1B Antibody Picoband&reg;Anti-BMPR1B antibody&#44; PA2017&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human BMPR1B&#44; 49.0KD (162aa tag+D17-D289)<br>Lane 1: Recombinant Human BMPR1B Protein 10ng <br>Lane 2: Recombinant Human BMPR1B Protein 5ng <br>Lane 3: Recombinant Human BMPR1B Protein 2.5ng <br>Lane 4: Recombinant Human BMPR1B Protein 1.25ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd68-antibody-pa2018-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2018-1-WB-anti-cd68-sr-d1-antibody.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Anti-CD68 antibody&#44; PA2018&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human CD68&#44; 42.5KD (162aa tag+ T128-L354)<br>Lane 1: Recombinant Human CD68 Protein 10ng <br>Lane 2: Recombinant Human CD68 Protein 5ng <br>Lane 3: Recombinant Human CD68 Protein 2.5nghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2018-2-IHC-anti-cd68-sr-d1-antibody.jpgAnti-Macrosialin CD68 Antibody Picoband&reg;Anti-CD68 antibody&#44; PA2018&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cetp-antibody-pa2019-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2019-1-WB-anti-cetp-antibody.jpgAnti-CETP Antibody Picoband&reg;Anti-CETP antibody&#44; PA2019&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br>Lane 5: RAJI Cell Lysate<br>Lane 6: MCF-7 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcr5-antibody-pa2021-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2021-1-WB-anti-cxcr5-blr1-antibody.jpgAnti-CXCR5 Antibody Picoband&reg;Anti-CXCR5 antibody&#44; PA2021&#44; Western blotting<br>Lane 1: Rat Spleen Tissue Lysate<br>Lane 2: HELA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp2u1-antibody-pa2022-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2022-1-WB-anti-cyp2u1-antibody.jpgAnti-Cytochrome P450 2U1 CYP2U1 Antibody Picoband&reg;Anti-CYP2U1 antibody&#44; PA2022&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: MM453 Cell Lysate<br>Lane 4: COLO320 Cell Lysate<br>Lane 5: HT1080 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2022-2-IHC-anti-cyp2u1-antibody.jpgAnti-Cytochrome P450 2U1 CYP2U1 Antibody Picoband&reg;Anti-CYP2U1 antibody&#44; PA2022&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2022-3-IF-anti-cyp2u1-antibody.jpgAnti-Cytochrome P450 2U1 CYP2U1 Antibody Picoband&reg;Anti-CYP2U1 antibody&#44; PA2022&#44; ICC<br>ICC: HELA Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-disc1-antibody-pa2023-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2023-1-WB-anti-disc1-antibody.jpgAnti-DISC1 Antibody Picoband&reg;Anti-DISC1 antibody&#44; PA2023&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: SHG Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2023-disc1-primary-antibodies-wb-testing-2.jpgAnti-DISC1 Antibody Picoband&reg; Western blot analysis of DISC1 using anti-DISC1 antibody (PA2023). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with anti-DISC1 antibody (PA2023) at 1:1000 overnight at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a anti-rabbit HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using a chemiluminescence. A specific band was detected for DISC1 at approximately 100 kDa. The expected band size for DISC1 is at 100 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-disc1-antibody-pa2023-1-boster.html2026-03-24T05:04:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2023-1-1_1-WB-anti-disc1-antibody.jpgAnti-DISC1 Antibody Picoband&reg; Western blot analysis of DISC1 using anti-DISC1 antibody (PA2023-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: U87 Whole Cell Lysate.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DISC1antigen affinity purified polyclonal antibody (Catalog # PA2023-1) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DISC1 at approximately 94KD. The expected band size for DISC1 is at 94KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2023-1-3_1-IHC-anti-disc1-antibody.jpgAnti-DISC1 Antibody Picoband&reg; IHC analysis of DISC1using anti-DISC1 antibody (PA2023-1).<br> DISC1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DISC1 Antibody (PA2023-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2023-1-2_1-IHC-anti-disc1-antibody.jpgAnti-DISC1 Antibody Picoband&reg; IHC analysis of DISC1using anti-DISC1 antibody (PA2023-1). DISC1 was detected in paraffin-embedded section of rat cerebellum tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DISC1 Antibody (PA2023-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gcn2-antibody-pa2028-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2028-1-WB-anti-gcn2-antibody.jpgAnti-GCN2/EIF2AK4 Antibody Picoband&reg;Anti-GCN2 antibody&#44; PA2028&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Mouse EIF2AK4&#44; 35.3KD (162aa tag+ E801-R954)<br>Lane 1: Recombinant Mouse EIF2AK4 Protein 10ng <br>Lane 2: Recombinant Mouse EIF2AK4 Protein 5ng <br>Lane 3: Recombinant Mouse EIF2AK4 Protein 2.5ng<br>Lane 4: Recombinant Mouse EIF2AK4 Protein 1.25ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif2s1-antibody-pa2029-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2029-1-WB-anti-eif2s1-eif2alpha-antibody.jpgAnti-EIF2S1 Antibody Picoband&reg;Anti-EIF2S1 antibody&#44; PA2029&#44; Western blotting<br>Lane 1: COLO320 Cell Lysate<br>Lane 2: CEM Cell Lysate<br>Lane 3: RAJI Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2029-2-IHC-anti-eif2s1-eif2alpha-antibody.jpgAnti-EIF2S1 Antibody Picoband&reg;Anti-EIF2S1 antibody&#44; PA2029&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2029-3-IHC-anti-eif2s1-eif2alpha-antibody.jpgAnti-EIF2S1 Antibody Picoband&reg;Anti-EIF2S1 antibody&#44; PA2029&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2029-ajcr0006-0924-f1.jpgAnti-EIF2S1 Antibody Picoband&reg;rL-RVG induces the expression of ER stress-related proteins in SGC-7901 and HGC cells in a time-dependent manner. A and D. For SGC; B and E. For HGC. The cells were infected with 10 MOI virus or with vehicle (control) for different amounts of time. Cell lysates were harvested at the same time after infection with virus for different amounts of time, and proteins were detected by western blotting. A and D. The expression of GRP78 and CHOP increased at 12 h after treatment with rL-RVG, peaked at 24 h (**, p<0.01) and was sustained for at least 12 h in SGC cells. The same was observed in B and E. for HGC cells. A transient increase in eIF2α phosphorylation following rL-RVG infection was observed at 24 h in SGC cells (**, p<0.01) and at 12 h in HGC cells (**, p<0.01). C. Effect of rL-RVG on the level of spliced mRNA forms of XBP-1. uXBP1, unspliced XBP-1; sXBP-1, spliced XBP-1. The spliced XBP-1 mRNA appeared with 12 h of pretreatment, but the effect was transient.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4889710/&orig_host=www.ncbi.nlm.nih.gov'>27293989</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2029-ajcr0006-0924-f3.jpgAnti-EIF2S1 Antibody Picoband&reg;NDV and rL-RVG each induce the expression of ER stress-related proteins in SGC-7901 and HGC cells in a concentration-dependent manner. A and C. For SGC; B and D. For HGC. The cells were infected with 10 MOI or 1 MOI virus for 24 h. NDV and rL-RVG infection increased the amounts of GRP78 and CHOP in SGC and HGC cells at 24 h post-infection, and the rL-RVG-infected group exhibited the strongest expression. Activation of the three branches of ER stress signaling pathway proteins, or XBP-1, ATF6 and p-eIF2α, increased significantly in SGC and HGC cells (*, p<0.05; **, p<0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4889710/&orig_host=www.ncbi.nlm.nih.gov'>27293989</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2029-ajcr0006-0924-f7.jpgAnti-EIF2S1 Antibody Picoband&reg;N+3: NDV+3MA; R+3: rL-RVG+3MA. The contribution of autophagy downregulation to the expression of ER stress-related proteins in SGC cells after infection. The expression of the ER stress-related proteins GRP78, CHOP and p-eIF2α decreased in cells after 3MA treatment compared with cells without 3MA treatment (*, p<0.05; **, p<0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4889710/&orig_host=www.ncbi.nlm.nih.gov'>27293989</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif3b-antibody-pa2030-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2030-eif3b-primary-antibodies-wb-testing-1_1.jpgAnti-eIF3B Antibody Picoband&reg; Western blot analysis of EIF3B using anti-EIF3B antibody (PA2030). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A375 whole cell lysates, <br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3B antigen affinity purified polyclonal antibody (Catalog # PA2030) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3B at approximately 115 kDa. The expected band size for EIF3B is at 92 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif6-antibody-pa2031-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2031-1-WB-anti-eif6-antibody.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg;Anti-EIF6 antibody&#44; PA2031&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Rat Kidney Tissue Lysate<br>Lane 3: COLO320 Cell Lysate<br>Lane 4: SW620 Cell Lysate<br>Lane 5: HELA Cell Lysate<br>Lane 6: 293T Cell Lysate <br>Lane 7: HEPA Cell Lysate https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2031-2-IHC-anti-eif6-antibody.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg;Anti-EIF6 antibody&#44; PA2031&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2031-3-IHC-anti-eif6-antibody.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg;Anti-EIF6 antibody&#44; PA2031&#44; IHC(F)<br>IHC(F): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-flotillin-1-antibody-pa2033-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-wb-testing-1_1.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg; Western blot analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: human PC-3 whole cell lysates,<br> Lane 4: human Hacat whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat skin tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse skin tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FLOT1 antigen affinity purified polyclonal antibody (Catalog # PA2033) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FLOT1 at approximately 47 kDa. The expected band size for FLOT1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ihc-testing-2_1.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg; IHC analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of human stomach camcer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ihc-testing-3_1.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg; IHC analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ihc-testing-4.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg; IHC analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ihc-testing-5.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg; IHC analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ihc-testing-6.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg;IHC analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ihc-testing-7.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg;IHC analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ihc-testing-8.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg;IHC analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-if-testing-1.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg;IF analysis of FLOT1 using anti-FLOT1 antibody (PA2033). <br> FLOT1 was detected in a paraffin-embedded section of human non-small cell lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FLOT1 Antibody (PA2033) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2033-flot1-primary-antibodies-ip-testing-6.jpgAnti-Flotillin 1/FLOT1 Antibody Picoband&reg; Immunoprecipitating FLOT1 in A431 whole cell lysate.<br> Western blot analysis of FLOT1 using anti-FLOT1 antibody (PA2033).<br> Lane 1: A431 whole cell lysates (30ug),<br> Lane 2: Rabbit control IgG instead of anti-FLOT1 antibody in A431 whole cell lysate,<br> Lane 3: anti-FLOT1 antibody (2μg) + A431 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FLOT1 antigen affinity purified polyclonal antibody (PA2033) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for FLOT1 at approximately 47 kDa. The expected band size for FLOT1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikk-beta-antibody-pa2036-1-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2036-1-1-WB-anti-ikk-beta-antibody.jpgAnti-IKK beta/IKBKB Antibody Picoband&reg;Anti-IKBKB antibody&#44; PA2036-1&#44; Western blotting<br>All lanes: Anti IKBKB (PA2036-1) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Lane 3: PANC Whole Cell Lysate at 40ug<br>Lane 4: MCF-7Whole Cell Lysate at 40ug<br>Lane 5: HEPG2 Whole Cell Lysate at 40ug<br>Lane 6: COLO320 Cell Lysate at 40ug<br>Predicted bind size: 87KD<br>Observed bind size: 87KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-18-antibody-pa2037-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2037-1-IHC-anti-il-18-antibody.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Anti-IL-18 antibody&#44; PA2037&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2037-2-WB-anti-il-18-antibody.jpgAnti-Interleukin-18 IL18 Antibody Picoband&reg;Anti-IL-18 antibody&#44; PA2037&#44; Western blotting<br>All lanes: Anti IL-18 (PA2037) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: Human Placenta Tissue Lysate at 50ug<br>Predicted bind size: 22KD<br>Observed bind size: 22KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-1-antibody-pa2038-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2038-1-IHC-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Anti-Kallikrein 1 antibody&#44; PA2038&#44; IHC(P)<br>IHC(P): Mouse Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2038-2-WB-anti-klk1-kallikrein-1-antibody.jpgAnti-Kallikrein 1/KLK1 Antibody Picoband&reg;Anti-Kallikrein 1 antibody&#44; PA2038&#44;Western blotting<br>All lanes: Anti Kallikrein 1 (PA2038) at 0.5ug/ml<br>WB: Mouse Testis Tissue Lysate at 50ug<br>Predicted bind size: 29KD<br>Observed bind size: 29KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ntal-antibody-pa2039-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2039-1-WB-anti-ntal-antibody.jpgAnti-NTAL/LAT2 Antibody Picoband&reg;Anti-NTAL antibody&#44; PA2039&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human LAT2&#44; 37.8KD (162aa tag+ M1-A182)<br>Lane 1: Recombinant Human LAT2 Protein 10ng <br>Lane 2: Recombinant Human LAT2 Protein 5ng <br>Lane 3: Recombinant Human LAT2 Protein 2.5ng <br>Lane 4: Recombinant Human LAT2 Protein 1.25ng https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lpp-antibody-pa2040-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2040-lpp-primary-antibodies-wb-testing-1.jpgAnti-Lipoma-preferred partner LPP Antibody Picoband&reg; Western blot analysis of LPP using anti-LPP antibody (PA2040). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPP antigen affinity purified polyclonal antibody (Catalog # PA2040) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPP at approximately 80 kDa. The expected band size for LPP is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mbd4-antibody-pa2042-boster.html2026-03-24T05:04:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2042-1-WB-anti-mbd4-antibody.jpgAnti-MBD4 Antibody Picoband&reg;Anti-MBD4 antibody&#44; PA2042&#44; Western blotting<br>Recombinant Protein Detection Source: E.coli derived -recombinant Human MBD4&#44; 39.7KD (162aa tag+ Q400-S580)<br>Lane 1: Recombinant Human MBD4 Protein 10ng <br>Lane 2: Recombinant Human MBD4 Protein 5ng <br>Lane 3: Recombinant Human MBD4 Protein 2.5ng https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2042-2-WB-anti-mbd4-antibody.jpgAnti-MBD4 Antibody Picoband&reg;Anti-MBD4 antibody&#44; PA2042&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Rat Kidney Tissue Lysate<br>Lane 3: A549 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: MCF-7 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-padi4-pad4-antibody-pa2043-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2043-1-WB-anti-padi4-pad4-antibody.jpgAnti-PADI4/PAD4 Antibody Picoband&reg;Anti-PADI4/PAD4 antibody&#44; PA2043&#44; Western blotting<br>Lane 1: PANC Cell Lysate<br>Lane 2: 293T Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pgk1-antibody-pa2045-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2045-pgk1-primary-antibodies-wb-testing-1.jpgAnti-Phosphoglycerate kinase 1 PGK1 Antibody Picoband&reg; Western blot analysis of PGK1 using anti-PGK1 antibody (PA2045). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: rat brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGK1 antigen affinity purified polyclonal antibody (Catalog # PA2045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGK1 at approximately 45 kDa. The expected band size for PGK1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2045-2_1-IHC-anti-pgk1-antibody.jpgAnti-Phosphoglycerate kinase 1 PGK1 Antibody Picoband&reg; IHC analysis of PGK1 using anti-PGK1 antibody (PA2045). <br> PGK1 was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PGK1 Antibody (PA2045) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkm2-antibody-pa2046-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2046-pkm2-primary-antibodies-wb-testing-1.jpgAnti-PKM2 Antibody Picoband&reg; Western blot analysis of PKM2 using anti-PKM2 antibody (PA2046). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKM2 antigen affinity purified polyclonal antibody (Catalog # PA2046) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKM2 at approximately 58 kDa. The expected band size for PKM2 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pla2g4a-antibody-pa2047-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2047-1-WB-anti-pla2g4a-antibody.jpgAnti-Phospholipase A2/PLA2G4A Antibody Picoband&reg;Anti-PLA2G4A antibody&#44; PA2047&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: A549 Cell Lysate<br>Lane 4: COLO320 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-periostin-antibody-pa2048-boster.html2026-04-01T05:01:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2048-1-WB-anti-periostin-osf2-antibody.jpgAnti-Periostin/POSTN Antibody Picoband&reg;Anti-Periostin antibody&#44; PA2048&#44; Western blotting<br>All lanes: Anti Periostin (PA2048) at 0.5ug/ml<br>WB: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Predicted bind size: 93KD<br>Observed bind size: 93KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rgs3-antibody-pa2050-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2050-1-WB-anti-rgs3-antibody.jpgAnti-RGS3 Antibody Picoband&reg;Anti-RGS3 antibody&#44; PA2050&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: Rat Spleen Tissue Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: U87 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2050-2-IHC-anti-rgs3-antibody.jpgAnti-RGS3 Antibody Picoband&reg;Anti-RGS3 antibody&#44; PA2050&#44; IHC(P)<br>IHC(P): Rat Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rip-antibody-pa2051-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2051-ripk1-primary-antibodies-wb-testing-1.jpgAnti-RIP/RIPK1 Antibody Picoband&reg; Western blot analysis of RIPK1 using anti-RIPK1 antibody (PA2051). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIPK1 antigen affinity purified polyclonal antibody (Catalog # PA2051) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIPK1 at approximately 76 kDa. The expected band size for RIPK1 is at 76 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rsk1-p90-antibody-pa2052-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2052-1-WB-anti-rsk1-p90-antibody.jpgAnti-RSK1 p90/RPS6KA1 Antibody Picoband&reg;Anti-RSK1 p90 antibody&#44; PA2052&#44; Western blotting<br>Lane 1: MCF-7 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: K562 Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br>Lane 5: SW620 Cell Lysate<br>Lane 6: RAJI Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2052-2-WB-anti-rsk1-p90-antibody.jpgAnti-RSK1 p90/RPS6KA1 Antibody Picoband&reg;Anti-RSK1 p90 antibody&#44; PA2052&#44; All Western blotting<br>All lanes: Anti-RPS6KA1 (PA2052) at 0.5ug/ml <br>Lane 1: A431 Whole Cell Lysate at 40ug <br>Lane 2: MCF-7 Whole Cell Lysate at 40ug <br>Lane 3: HELA Whole Cell Lysate at 40ug <br>Predicted bind size: 83KD <br>Observed bind size: 83KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p53r2-antibody-pa2053-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2053-1-WB-anti-p53r2-antibody.jpgAnti-p53R2/RRM2B Antibody Picoband&reg;Anti-p53R2 antibody&#44; PA2053&#44; Western blotting<br>Lane 1: Rat Thymus Tissue Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: A431 Cell Lysate<br>Lane 4: HELA Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-somatostatin-antibody-pa2054-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2054-1-WB-anti-somatostatin-antibody.jpgAnti-Somatostatin/SST Antibody Picoband&reg;Anti-Somatostatin antibody&#44; PA2054&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: SW620 Cell Lysate<br>Lane 4: MCF-7 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2054-2-IHC-anti-somatostatin-antibody.jpgAnti-Somatostatin/SST Antibody Picoband&reg;Anti-Somatostatin antibody&#44; PA2054&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p63-antibody-pa2056-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2056-p63-primary-antibodies-wb-testing-1.jpgAnti-p63/TP63 Antibody Picoband&reg; Western blot analysis of p63 using anti-p63 antibody (PA2056). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p63 antigen affinity purified polyclonal antibody (Catalog # PA2056) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p63 at approximately 75 kDa. The expected band size for p63 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2056-2-IHC-anti-p63-antibody.jpgAnti-p63/TP63 Antibody Picoband&reg; IHC analysis of p63 using anti-p63 antibody (PA2056). <br> p63 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-p63 Antibody (PA2056) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2056-3-IHC-anti-p63-antibody.jpgAnti-p63/TP63 Antibody Picoband&reg; IHC analysis of p63 using anti-p63 antibody (PA2056). <br> p63 was detected in a paraffin-embedded section of human esophageal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-p63 Antibody (PA2056) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2056-4-IHC-anti-p63-antibody.jpgAnti-p63/TP63 Antibody Picoband&reg; IHC analysis of p63 using anti-p63 antibody (PA2056). <br> p63 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-p63 Antibody (PA2056) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2056-5-IF-anti-p63-antibody.jpgAnti-p63/TP63 Antibody Picoband&reg; ICC analysis of p63 using anti-p63 antibody (PA2056). <br> p63 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-p63 Antibody (PA2056) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2056-tp63-primary-antibodies-if-testing-6_1.jpgAnti-p63/TP63 Antibody Picoband&reg; IF analysis of TP63 using anti-TP63 antibody (PA2056). <br> TP63 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-TP63 Antibody (PA2056) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2056-p63-primary-antibodies-if-testing-2.jpgAnti-p63/TP63 Antibody Picoband&reg;IF analysis of TP63 using anti-TP63 antibody (PA2056). <br> TP63 was detected in an immunocytochemical section of human Hela cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with rabbit anti-TP63 Antibody (PA2056) at a dilution of 1:50 overnight at 4°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2056-tp63-primary-antibodies-fcm-testing-7_1.pngAnti-p63/TP63 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-TP63 antibody (PA2056). <br>Overlay histogram showing A431 cells stained with PA2056 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TP63 Antibody (PA2056, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd244-antibody-pa2057-boster.html2026-03-24T05:04:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2057-1-WB-anti-cd244-2b4-antibody.jpgAnti-CD244 Antibody Picoband&reg;Anti-CD244 antibody&#44; PA2057&#44; Western blotting<br>All lanes: Anti CD244 (PA2057) at 0.5ug/ml<br>Lane 1: MCF-7 Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 42KD<br>Observed bind size: 42KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2057-2-IHC-anti-cd244-2b4-antibody.jpgAnti-CD244 Antibody Picoband&reg;Anti-CD244 antibody&#44; PA2057&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prothrombin-antibody-pa2059-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2059-1-WB-anti-prothrombin-antibody.jpgAnti-Prothrombin/F2 Antibody Picoband&reg;Anti-F2 antibody&#44; PA2059&#44; Western blotting<br>All lanes: Anti F2 (PA2059) at 0.5ug/ml<br>WB : Rat Testis Tissue Lysate at 50ug<br>Predicted bind size: 70KD<br>Observed bind size: 100KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-factor-vii-antibody-pa2060-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2060-1-WB-anti-factor-vii-antibody.jpgAnti-Factor VII/F7 Antibody Picoband&reg;Anti-Factor VII antibody&#44; PA2060&#44; Western blotting<br>All lanes: Anti Factor VII (PA2060) at 0.5ug/ml<br>Lane 1: SMMC Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Lane 3: RAJI Whole Cell Lysate at 40ug<br>Predicted bind size: 52KD<br>Observed bind size: 52KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-factor-viii-antibody-pa2061-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2061-1_1.jpgAnti-Factor VIII/F8 Antibody Picoband&reg; Western blot analysis of Factor VIII using anti-Factor VIII antibody (PA2061). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates&#44; <br> Lane 2: rat lung tissue lysates&#44; <br> Lane 3: rat heart tissue lysates&#44; <br> Lane 4: mouse kidney tissue lysates&#44; <br> Lane 5: mouse lung tissue lysates&#44; <br> Lane 6: human Hela whole cell lysates&#44; <br> Lane 7: human HepG2 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor VIII antigen affinity purified polyclonal antibody (Catalog # PA2061) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor VIII at approximately 92KD. The expected band size for Factor VIII is at 92KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc2a5-antibody-pa2064-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2064-2-WB-anti-slc2a5-glut5-antibody.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg;Anti-SLC2A5 antibody&#44; PA2064&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: 293T Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br>Lane 4: SW620 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abca4-antibody-pa2065-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2065-1-WB-anti-abca4-antibody.jpgAnti-ABCA4 Antibody Picoband&reg;Anti-ABCA4 antibody&#44; PA2065&#44; Western blotting<br>WB: U87 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abca4-antibody-pa2066-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2066-abca4-primary-antibodies-wb-testing-1.jpgAnti-ABCA4 Antibody Picoband&reg; Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse eye tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcg4-antibody-pa2067-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2067-1-WB-anti-abcg4-antibody.jpgAnti-ABCG4 Antibody Picoband&reg;Anti-ABCG4 antibody&#44; PA2067&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abi2-antibody-pa2068-boster.html2026-04-01T05:01:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2068-abi2-primary-antibodies-wb-testing-1.jpgAnti-Abl interactor 2 ABI2 Antibody Picoband&reg;Western blot analysis of ABI2 using anti-ABI2 antibody (PA2068). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABI2 antigen affinity purified polyclonal antibody (Catalog # PA2068) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ABI2 at approximately 56-70 kDa. The expected band size for ABI2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2068-abi2-primary-antibodies-if-testing-1.jpgAnti-Abl interactor 2 ABI2 Antibody Picoband&reg;IF analysis of ABI2 using anti-ABI2 antibody (PA2068) and anti-Tubulin Alpha antibody (M03989-3).<br> ABI2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABI2 Antibody (PA2068) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2068-abi2-primary-antibodies-fcm-testing-1.jpgAnti-Abl interactor 2 ABI2 Antibody Picoband&reg;Flow Cytometry analysis of SH-SY5Y cells using anti-ABI2 antibody (PA2068). <br> Overlay histogram showing SH-SY5Y cells stained with PA2068 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABI2 Antibody (PA2068, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adam2-antibody-pa2069-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2069-1-WB-anti-adam2-antibody.jpgAnti-ADAM2 Antibody Picoband&reg;Anti-ADAM2 antibody&#44; PA2069&#44; Western blotting<br>WB: SMMC Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adam19-antibody-pa2070-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2070-adam19-primary-antibodies-wb-testing-1.jpgAnti-ADAM19 Antibody Picoband&reg; Western blot analysis of ADAM19 using anti-ADAM19 antibody (PA2070). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human U87 whole cell lysates,<br> Lane 4: human U20S whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAM19 antigen affinity purified polyclonal antibody (Catalog # PA2070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAM19 at approximately 105 kDa. The expected band size for ADAM19 is at 105 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adamts1-antibody-pa2071-boster.html2026-03-24T05:04:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2071-1_1-WB-anti-adamts1-antibody.jpgAnti-ADAMTS1 Antibody Picoband&reg;Anti-ADAMTS1 antibody&#44; PA2071&#44; Western blotting<br>All lanes: Anti ADAMTS1 (PA2071) at 0.5ug/ml<br> Lane 1: Rat Liver Tissue Lysate at 50ug<br> Lane 2: Rat Heart Tissue Lysate at 50ug<br> Lane 3: Rat Brain Tissue Lysate at 50ug<br> Predicted bind size: 105KD<br> Observed bind size: 105KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh2-antibody-pa2073-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2073-1-WB-anti-aldh2-antibody.jpgAnti-ALDH2 Antibody Picoband&reg;Anti-ALDH2 antibody&#44; PA2073&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Rat Intestine Tissue Lysate<br>Lane 3: Rat Lung Tissue Lysate<br>Lane 4: Rat Kidney Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2073-2-IHC-anti-aldh2-antibody.jpgAnti-ALDH2 Antibody Picoband&reg;Anti-ALDH2 antibody&#44; PA2073&#44; IHC(P)<br>IHC(P): Human Liver Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2073-3.jpgAnti-ALDH2 Antibody Picoband&reg; IHC analysis of ALDH2 using anti-ALDH2 antibody (PA2073). <br> ALDH2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH2 Antibody (PA2073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh3a1-antibody-pa2074-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2074-aldh3a1-primary-antibodies-wb-testing-1.jpgAnti-ALDH3A1 Antibody Picoband&reg; Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (PA2074). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # PA2074) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 50 kDa. The expected band size for ALDH3A1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2074-aldh3a1-primary-antibodies-ihc-testing-2.jpgAnti-ALDH3A1 Antibody Picoband&reg; IHC analysis of ALDH3A1 using anti-ALDH3A1 antibody (PA2074). <br> ALDH3A1 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH3A1 Antibody (PA2074) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angptl3-antibody-pa2075-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2075-1-WB-anti-angptl3-antibody.jpgAnti-Angiopoietin-related protein 3 ANGPTL3 Antibody Picoband&reg;Anti-ANGPTL3 antibody&#44; PA2075&#44; Western blotting<br>WB: A549 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-vii-antibody-pa2076-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2076-anxa7-primary-antibodies-wb-testing-1_1.jpgAnti-Annexin VII/ANXA7 Antibody Picoband&reg; Western blot analysis of ANXA7 using anti-ANXA7 antibody (PA2076). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates, <br> Lane 2: human SW620 whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: human A549 whole cell lysates, <br> Lane 6: human Raji whole cell lysates, <br> Lane 7: human HEK293 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA7 antigen affinity purified polyclonal antibody (Catalog # PA2076) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANXA7 at approximately 50 kDa. The expected band size for ANXA7 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2076-anxa7-primary-antibodies-fcm-testing-4.pngAnti-Annexin VII/ANXA7 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-ANXA7 antibody (PA2076). <br>Overlay histogram showing HepG2 cells stained with PA2076 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANXA7 Antibody (PA2076, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2076-2-ihc-anti-annexin-vii-antibody.jpgAnti-Annexin VII/ANXA7 Antibody Picoband&reg; IHC analysis of ANXA7 using anti-ANXA7 antibody (PA2076). <br> ANXA7 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ANXA7 Antibody (PA2076) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2076-anxa7-primary-antibodies-if-testing-3.jpgAnti-Annexin VII/ANXA7 Antibody Picoband&reg; IF analysis of ANXA7 using anti- ANXA7 antibody (PA2076). <br> ANXA7 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- ANXA7 Antibody (PA2076) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a10-antibody-pa2077-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2077-1-WB-anti-annexin-a10-antibody.jpgAnti-Annexin A10/ANXA10 Antibody Picoband&reg;Anti-Annexin A10 antibody&#44; PA2077&#44; Western blotting<br>Lane 1: A549 Cell Lysate<br>Lane 2: A549 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2077-2-IHC-anti-annexin-a10-antibody.jpgAnti-Annexin A10/ANXA10 Antibody Picoband&reg;Anti-Annexin A10 antibody&#44; PA2077&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ve-cadherin-antibody-pa2079-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2079-1-WB-anti-ve-cadherin-cd144-antibody.jpgAnti-VE-Cadherin CDH5-Antibody Picoband&reg;Anti-VE Cadherin antibody&#44; PA2079&#44; Western blotting<br>All lanes: Anti VE Cadherin (PA2079) at 0.5ug/ml<br> Lane 1: A549 Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 87KD<br> Observed bind size: 130KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2079-2-IHC-anti-ve-cadherin-cd144-antibody.jpgAnti-VE-Cadherin CDH5-Antibody Picoband&reg;Anti-VE Cadherin antibody&#44; PA2079&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2079-3-IHC-anti-ve-cadherin-cd144-antibody.jpgAnti-VE-Cadherin CDH5-Antibody Picoband&reg;Anti-VE Cadherin antibody&#44; PA2079&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2079-dddt-12-3951fig3.jpgAnti-VE-Cadherin CDH5-Antibody Picoband&reg;VPA promoted angiogenesis in random skin flaps. Notes: ( A ) In situ hybridization of VEGF mRNA on day 7 (original magnification ×400). ( B ) The optical density values of VEGF mRNA. ( C and E ) VEGF and cadherin 5 expressions in each group as assessed by IHC (original magnification ×200). ( D and F ) The optical density values of VEGF and cadherin 5. ( G ) Protein expression of VEGF in each group, as assessed by Western blot analysis. The gels have been run under the same experimental conditions, and cropped blots are used here. ( H ) Densitometry results of VEGF protein expression in the two groups. Values are expressed as mean ± SEM, n=6 per group. * P <0.05 and ** P <0.01, vs control group. Abbreviations: IHC, immunohistochemistry; SEM, standard error of mean; VEGF, vascular endothelial growth factor; VPA, valproic acid.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6248271/&orig_host=www.ncbi.nlm.nih.gov'>30510403</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2079-ve-cadherin-primary-antibodies-wb-testing-1.jpgAnti-VE-Cadherin CDH5-Antibody Picoband&reg;Western blot analysis of CDH5 using anti-CDH5 antibody (PA2079). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SK-HEP1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH5 antigen affinity purified polyclonal antibody (PA2079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDH5 at approximately 120 kDa. The expected band size for CDH5 is at 88 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2079-ve-cadherin-primary-antibodies-icc-testing-1.jpgAnti-VE-Cadherin CDH5-Antibody Picoband&reg;IF analysis of CDH5 using anti-CDH5 antibody (PA2079). <br> CDH5 was detected in an immunocytochemical section of human SK-HEP1 cells. Cells were permeabilized with Triton X-100 for 10 minutes. The cells were blocked with 10% goat serum. And then incubated with 10 μg/mL rabbit anti-CDH5 Antibody (PA2079) overnight at 4°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127), Cy3 conjugated goat anti-mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcr1-antibody-pa2080-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2080-fphar-10-00307-g001.jpgAnti-CXCR1 Antibody Picoband&reg;Expressions of CXCL6, CXCR1, and CXCR2 in osteosarcoma (OS) cells. The mRNA expressions of CXCL6 (A) , CXCR1 (B) , and CXCR2 (C) in multiple OS cell lines were evaluated by real-time PCR. The protein levels of CXCL6 (D) , CXCR1 (E) , and CXCR2 (F) in multiple OS cell lines were detected by western blot assay. (G–I) The protein quantification histograms were shown.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00307/full'>30984000</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2080-cxcr1-primary-antibodies-fcm-testing-2.jpgAnti-CXCR1 Antibody Picoband&reg; Flow Cytometry analysis of human PBMC cells using anti-CXCR1 antibody (PA2080). <br>Overlay histogram showing human PBMC cells stained with PA2080 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR1 Antibody (PA2080, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2080-cxcr1-primary-antibodies-wb-testing-1.jpgAnti-CXCR1 Antibody Picoband&reg; Western blot analysis of CXCR1 using anti-CXCR1 antibody (PA2080). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR1 antigen affinity purified polyclonal antibody (Catalog # PA2080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR1 at approximately 50 kDa. The expected band size for CXCR1 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcr4-antibody-pa2081-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2081-1-WB-anti-cxcr4-antibody.jpgAnti-CXCR4 Antibody Picoband&reg;Anti-CXCR4 antibody&#44; PA2081&#44; Western blotting<br>Lane 1: M231 Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bonzo-antibody-pa2082-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2082-1-IHC-anti-bonzo-antibody.jpgAnti-Bonzo/CXCR6 Antibody Picoband&reg;Anti-Bonzo antibody&#44; PA2082&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2082-2-WB-anti-bonzo-antibody.jpgAnti-Bonzo/CXCR6 Antibody Picoband&reg;Anti-Bonzo antibody&#44; PA2082&#44; Western blotting<br>All lanes: Anti Bonzo (PA2082) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Lane 3: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 39KD<br>Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-madcam1-antibody-pa2083-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2083-madcam1-primary-antibodies-wb-testing-1.jpgAnti-MAdCAM1 Antibody Picoband&reg; Western blot analysis of MAdCAM1 using anti-MAdCAM1 antibody (PA2083). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAdCAM1 antigen affinity purified polyclonal antibody (Catalog # PA2083) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAdCAM1 at approximately 60 kDa. The expected band size for MAdCAM1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2083-madcam1-primary-antibodies-ihc-testing-2.jpgAnti-MAdCAM1 Antibody Picoband&reg; IHC analysis of MAdCAM1 using anti-MAdCAM1 antibody (PA2083). <br> MAdCAM1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAdCAM1 Antibody (PA2083) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2083-madcam1-primary-antibodies-if-testing-3.jpgAnti-MAdCAM1 Antibody Picoband&reg; IF analysis of MAdCAM1 using anti-MAdCAM1 antibody (PA2083). <br> MAdCAM1 was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MAdCAM1 Antibody (PA2083) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pc1-3-antibody-pa2084-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2084-1-WB-anti-pc1-3-antibody.jpgAnti-PC1/3/PCSK1 Antibody Picoband&reg; Western blot analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody (PA2084). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Liver Tissue Lysate <br>Lane 2: Rat Thymus Tissue Lysate <br>Lane 3: A549 Cell Lysate <br>Lane 4: HELA Cell Lysate <br>Lane 5: COLO320 Cell Lysate <br>Lane 6: PANC Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC1/3/PCSK1 antigen affinity purified polyclonal antibody (Catalog # PA2084) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PC1/3/PCSK1 at approximately 84KD. The expected band size for PC1/3/PCSK1 is at 84KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2084-pcsk1-primary-antibodies-ihc-testing-3.jpgAnti-PC1/3/PCSK1 Antibody Picoband&reg;IHC analysis of PCSK1 using anti-PCSK1 antibody (PA2084). <br>PCSK1 was detected in a paraffin-embedded section of human pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCSK1 Antibody (PA2084) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2084-2-IHC-anti-pc1-3-antibody.jpgAnti-PC1/3/PCSK1 Antibody Picoband&reg; IHC analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody (PA2084). <br> PC1/3/PCSK1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PC1/3/PCSK1 Antibody (PA2084) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2084-3.jpgAnti-PC1/3/PCSK1 Antibody Picoband&reg; IF analysis of PC1/3/PCSK1 using anti-PC1/3/PCSK1 antibody (PA2084) <br> PC1/3/PCSK1 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-PC1/3/PCSK1 Antibody (PA2084) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-proprotein-convertase-pc4-antibody-pa2086-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2086-1_1.jpgAnti-Proprotein convertase PC4/PCSK4 Antibody Picoband&reg; Western blot analysis of PCSK4 using anti-PCSK4 antibody (PA2086). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody (Catalog # PA2086) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prlr-antibody-pa2087-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2087-2-IHC-anti-prlr-prolactin-r-antibody.jpgAnti-Prolactin Receptor/PRLR Antibody Picoband&reg;Anti-PRLR antibody&#44; PA2087&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2087-3-IHC-anti-prlr-prolactin-r-antibody.jpgAnti-Prolactin Receptor/PRLR Antibody Picoband&reg;Anti-PRLR antibody&#44; PA2087&#44; IHC(P)<br>IHC(P): Rat Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2087-1_1_1.jpgAnti-Prolactin Receptor/PRLR Antibody Picoband&reg; Western blot analysis of PRLR using anti-PRLR antibody (PA2087). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: rat PC-12 whole cell lysates. <br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PA2087) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2087-4.jpgAnti-Prolactin Receptor/PRLR Antibody Picoband&reg; Western blot analysis of PRLR using anti-PRLR antibody (PA2087). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44; <br> Lane 2: human MCF-7 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PA2087) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 95KD. The expected band size for PRLR is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2087-prlr-primary-antibodies-wb-testing-1.pngAnti-Prolactin Receptor/PRLR Antibody Picoband&reg; Western blot analysis of PRLR using anti-PRLR antibody (PA2087). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: control group-mouse brain tissue lysates,<br> Lane 2: model group-mouse brain tissue lysates,<br> Lane 3: high-dose A group-mouse brain tissue lysates,<br> Lane 4: low-dose A group-mouse brain tissue lysates,<br> Lane 5: high-dose B group-mouse brain tissue lysates,<br> Lane 6: low-dose B group-mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified monoclonal antibody (Catalog # PA2087) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) at a dilution of 1:2000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for PRLR at approximately 70 kDa. The expected band size for PRLR is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sparcl1-antibody-pa2088-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2088-1-IHC-anti-sparcl1-sparc-like-1-antibody.jpgAnti-SPARC-like protein 1 SPARCL1 Antibody Picoband&reg;Anti-SPARCL1 antibody&#44; PA2088&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2088-2-WB-anti-sparcl1-sparc-like-1-antibody.jpgAnti-SPARC-like protein 1 SPARCL1 Antibody Picoband&reg;Anti-SPARCL1 antibody&#44; PA2088&#44; Western blotting<br>Lane 1: Rat Lung Tissue Lysate<br>Lane 2: Mouse Lung Tissue Lysate<br>Lane 3: PANC Cell Lysate<br>Lane 4: A549 Cell Lysate<br>Lane 5: COLO320 Cell Lysate<br>Lane 6: MCF-7 Cell Lysate<br>Lane 7: HT1080 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2088-3-IHC-anti-sparcl1-sparc-like-1-antibody.jpgAnti-SPARC-like protein 1 SPARCL1 Antibody Picoband&reg;Anti-SPARCL1 antibody&#44; PA2088&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wisp1-antibody-pa2089-boster.html2026-03-24T05:04:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2089-wisp1-primary-antibodies-wb-testing-1.jpgAnti-WISP1 Antibody Picoband&reg; Western blot analysis of WISP1 using anti-WISP1 antibody (PA2089). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates,<br> Lane 2: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WISP1 antigen affinity purified polyclonal antibody (Catalog # PA2089) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WISP1 at approximately 45 kDa. The expected band size for WISP1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2089-wisp1-primary-antibodies-ihc-testing-2.jpgAnti-WISP1 Antibody Picoband&reg; IHC analysis of WISP1 using anti-WISP1 antibody (PA2089). <br> WISP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WISP1 Antibody (PA2089) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2089-wisp1-primary-antibodies-ihc-testing-3.jpgAnti-WISP1 Antibody Picoband&reg; IHC analysis of WISP1 using anti-WISP1 antibody (PA2089). <br> WISP1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WISP1 Antibody (PA2089) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcb4-antibody-pa2090-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2090-abcb4-primary-antibodies-wb-testing-1.jpgAnti-ABCB4 Antibody Picoband&reg; Western blot analysis of ABCB4 using anti-ABCB4 antibody (PA2090). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody (Catalog # PA2090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 142 kDa. The expected band size for ABCB4 is at 142 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pmp70-antibody-pa2091-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2091-abcd3-primary-antibodies-wb-testing-1.jpgAnti-PMP70/ABCD3 Antibody Picoband&reg;Western blot analysis of PMP70/ABCD3 using anti-PMP70/ABCD3 antibody (PA2091). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMP70/ABCD3 antigen affinity purified polyclonal antibody (PA2091) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PMP70/ABCD3 at approximately 70 kDa. The expected band size for PMP70/ABCD3 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2091-abcd3-primary-antibodies-wb-testing-2.jpgAnti-PMP70/ABCD3 Antibody Picoband&reg;Western blot analysis of PMP70/ABCD3 using anti-PMP70/ABCD3 antibody (PA2091). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Mock group-EV-monkey MARC-145 whole cell lysates,<br> Lane 2: Mock group-OE-monkey MARC-145 whole cell lysates,<br> Lane 3: Virus group-EV-monkey MARC-145 whole cell lysates,<br> Lane 4: Virus group-OE-monkey MARC-145 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PMP70/ABCD3 antigen affinity purified polyclonal antibody (PA2091) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP. A specific band was detected for PMP70/ABCD3 at approximately 70 kDa. The expected band size for PMP70/ABCD3 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2091-abcd3-primary-antibodies-ihc-testing-1.jpgAnti-PMP70/ABCD3 Antibody Picoband&reg;IHC analysis of PMP70/ABCD3 using anti-PMP70/ABCD3 antibody (PA2091). <br>PMP70/ABCD3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PMP70/ABCD3 Antibody (PA2091) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2091-abcd3-primary-antibodies-ihc-testing-2.jpgAnti-PMP70/ABCD3 Antibody Picoband&reg;IHC analysis of PMP70/ABCD3 using anti-PMP70/ABCD3 antibody (PA2091). <br>PMP70/ABCD3 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PMP70/ABCD3 Antibody (PA2091) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2091-abcd3-primary-antibodies-if-testing-1.jpgAnti-PMP70/ABCD3 Antibody Picoband&reg;IF analysis of PMP70/ABCD3 using anti-PMP70/ABCD3 antibody (PA2091). <br> PMP70/ABCD3 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PMP70/ABCD3 Antibody (PA2091) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-6-antibody-pa2092-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2092-1-WB-anti-aquaporin-6-antibody.jpgAnti-Aquaporin 6/AQP6 Antibody Picoband&reg;Anti-Aquaporin 6 antibody&#44; PA2092&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: MCF-7 Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2092-2-IHC-anti-aquaporin-6-antibody.jpgAnti-Aquaporin 6/AQP6 Antibody Picoband&reg;Anti-Aquaporin 6 antibody&#44; PA2092&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2092-3-IHC-anti-aquaporin-6-antibody.jpgAnti-Aquaporin 6/AQP6 Antibody Picoband&reg;Anti-Aquaporin 6 antibody&#44; PA2092&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-9-antibody-pa2094-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2094-1_1-WB-anti-aquaporin-9-antibody.jpgAnti-Aquaporin 9/AQP9 Antibody Picoband&reg;Anti-Aquaporin 9 antibody&#44; PA2094&#44; Western blotting<br>All lanes: Anti Aquaporin 9 (PA2094) at 0.5ug/ml<br> Lane 1: Mouse Liver Tissue Lysate at 50ug<br> Lane 2: Mouse Lung Tissue Lysate at 50ug<br> Lane 3: Mouse Spleen Tissue Lysate at 50ug<br> Lane 4: Mouse Testis Tissue Lysate at 50ug<br> Lane 5: PC-12 Whole Cell Lysate at 40ug<br> Predicted bind size: 31KD<br> Observed bind size: 31KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-10-antibody-pa2095-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2095-1-WB-anti-aquaporin-10-antibody.jpgAnti-Aquaporin 10/AQP10 Antibody Picoband&reg;Anti-Aquaporin 10 antibody&#44; PA2095&#44; Western blotting<br>WB: COLO320 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aplp1-antibody-pa2096-boster.html2026-03-27T05:07:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2096-1-IHC-anti-aplp1-antibody.jpgAnti-Amyloid-like protein 1 APLP1 Antibody Picoband&reg;Anti-APLP1 antibody&#44; PA2096&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2096-2-WB-anti-aplp1-antibody.jpgAnti-Amyloid-like protein 1 APLP1 Antibody Picoband&reg;Anti-APLP1 antibody&#44; PA2096&#44; Western blotting<br>All lanes: Anti APLP1 (PA2096) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: PC-12 Whole Cell Lysate at 40ug<br>Lane 3: HEPA Whole Cell Lysate at 40ug<br>Predicted bind size: 72KD<br>Observed bind size: 72KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2096-3-IHC-anti-aplp1-antibody.jpgAnti-Amyloid-like protein 1 APLP1 Antibody Picoband&reg;Anti-APLP1 antibody&#44; PA2096&#44; IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atx2-antibody-pa2098-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-wb-testing-1_1.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; Western blot analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat liver tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATXN2 antigen affinity purified polyclonal antibody (Catalog # PA2098) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATXN2 at approximately 140-150 kDa. The expected band size for ATXN2 is at 140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-ihc-testing-6.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg;IHC analysis of Ataxin 2/ATXN2 using anti-Ataxin 2/ATXN2 antibody (PA2098). <br>Ataxin 2/ATXN2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ataxin 2/ATXN2 Antibody (PA2098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-ihc-testing-1.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg;IHC analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> ATXN2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATXN2 Antibody (PA2098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-ihc-testing-2.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> ATXN2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATXN2 Antibody (PA2098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-ihc-testing-3.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> ATXN2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATXN2 Antibody (PA2098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-ihc-testing-4.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> ATXN2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATXN2 Antibody (PA2098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-ihc-testing-5.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> ATXN2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATXN2 Antibody (PA2098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-if-testing-6.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IF analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> ATXN2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATXN2 Antibody (PA2098) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-if-testing-7.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IF analysis of ATXN2 using anti-ATXN2 antibody (PA2098). <br> ATXN2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATXN2 Antibody (PA2098) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2098-atxn2-primary-antibodies-fcm-testing-8.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; Flow Cytometry analysis of RT4 cells using anti-ATXN2 antibody (PA2098). <br> Overlay histogram showing RT4 cells stained with ATXN2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATXN2 Antibody (PA2098, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bag2-antibody-pa2099-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2099-1-WB-anti-bag2-antibody.jpgAnti-BAG2 Antibody Picoband&reg;Anti-BAG2 antibody&#44; PA2099&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: A549 Cell Lysate<br>Lane 4: A431 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bag5-antibody-pa2101-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2101-bag5-primary-antibodies-fcm-testing-2.jpgAnti-Bag5 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-BAG5 antibody (PA2101). <br>Overlay histogram showing A431 cells stained with PA2101 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAG5 Antibody (PA2101, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2101-bag5-primary-antibodies-wb-testing-1.jpgAnti-Bag5 Antibody Picoband&reg; Western blot analysis of BAG5 using anti-BAG5 antibody (PA2101). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAG5 antigen affinity purified polyclonal antibody (Catalog # PA2101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAG5 at approximately 51 kDa. The expected band size for BAG5 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcl2a1-antibody-pa2102-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2102-1-WB-anti-bcl2a1-bcl-2-related-protein-a1-antibody.jpgAnti-Bcl-2-related protein A1 Bcl2A1 Antibody Picoband&reg;Anti-Bcl2A1 antibody&#44; PA2102&#44; Western blotting<br>All lanes: Anti Bcl2A1(PA2102) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 30KD<br>Observed bind size: 30KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-12-antibody-pa2103-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2103-1-WB-anti-caspase-12-antibody.jpgAnti-Caspase-12/CASP12 Antibody Picoband&reg;Anti-Caspase-12 antibody&#44; PA2103&#44; Western blotting<br>All lanes: Anti Caspase-12 (PA2103) at 0.5ug/ml<br> Lane 1: PANC Whole Cell Lysate at 40ug<br> Lane 2: SMMC Whole Cell Lysate at 40ug<br> Lane 3: A549 Whole Cell Lysate at 40ug<br> Lane 4: HELA Whole Cell Lysate at 40ug<br> Predicted bind size: 39KD<br> Observed bind size: 39KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2103-2-IHC-anti-caspase-12-antibody.jpgAnti-Caspase-12/CASP12 Antibody Picoband&reg;Anti-Caspase-12 antibody&#44; PA2103&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2103-3-IHC-anti-caspase-12-antibody.jpgAnti-Caspase-12/CASP12 Antibody Picoband&reg;Anti-Caspase-12 antibody&#44; PA2103&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-14-antibody-pa2104-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2104-1-WB-anti-caspase-14-antibody.jpgAnti-Caspase-14/CASP14 Antibody Picoband&reg;Anti-Caspase-14 antibody&#44; PA2104&#44; Western blotting<br>All lanes: Anti Caspase-14 (PA2104) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 50ug<br> Lane 2: Rat Liver Tissue Lysate at 50ug<br> Lane 3: Rat Spleen Tissue Lysate at 50ug<br> Lane 4: A431 Whole Cell Lysate at 40ug<br> Lane 5: NIH3T3 Whole Cell Lysate at 40ug<br> Predicted bind size: 28KD<br> Observed bind size: 28KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2104-2-IHC-anti-caspase-14-antibody.jpgAnti-Caspase-14/CASP14 Antibody Picoband&reg;Anti-Caspase-14 antibody&#44; PA2104&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2104-3-IHC-anti-caspase-14-antibody.jpgAnti-Caspase-14/CASP14 Antibody Picoband&reg;Anti-Caspase-14 antibody&#44; PA2104&#44; IHC(P)<br>IHC(P): Human Oesophagus Squama Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cystatin-a-antibody-pa2105-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2105-1_2.jpgAnti-Cystatin A/CSTA Antibody Picoband&reg; Western blot analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody (PA2105).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A/CSTA antigen affinity purified polyclonal antibody (Catalog # PA2105) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystatin A/CSTA at approximately 14KD. The expected band size for Cystatin A/CSTA is at 11KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2105-2-IHC-anti-cystatin-a-antibody.jpgAnti-Cystatin A/CSTA Antibody Picoband&reg; IHC analysis of Cystatin A/CSTA using anti-Cystatin A/CSTA antibody (PA2105).<br> Cystatin A/CSTA was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cystatin A/CSTA Antibody (PA2105) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stefin-b-antibody-pa2106-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2106-cstb-primary-antibodies-wb-testing-1.jpgAnti-Stefin B/CSTB Antibody Picoband&reg;Western blot analysis of CSTB using anti-CSTB antibody (PA2106). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSTB antigen affinity purified polyclonal antibody (PA2106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CSTB at approximately 11 kDa. The expected band size for CSTB is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2106-cstb-primary-antibodies-ihc-testing-1.jpgAnti-Stefin B/CSTB Antibody Picoband&reg;IHC analysis of CSTB using anti-CSTB antibody (PA2106). <br>CSTB was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSTB Antibody (PA2106) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fmo5-antibody-pa2108-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2108-fmo5-primary-antibodies-wb-testing-1.jpgAnti-FMO5 Antibody Picoband&reg; Western blot analysis of FMO5 using anti-FMO5 antibody (PA2108). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO5 antigen affinity purified polyclonal antibody (Catalog # PA2108) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO5 at approximately 60 kDa. The expected band size for FMO5 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gelsolin-antibody-pa2109-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2109-gsn-primary-antibodies-wb-testing-1.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; Western blot analysis of Gelsolin using anti-Gelsolin antibody (PA2109). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human THP-1 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: monkey COS-7 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin antigen affinity purified polyclonal antibody (Catalog # PA2109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 86 kDa. The expected band size for Gelsolin is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2109-2-IHC-anti-gelsolin-antibody.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PA2109).<br> Gelsolin/GSN was detected in paraffin-embedded section of Human Lung Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gelsolin/GSN Antibody (PA2109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2109-3-IHC-anti-gelsolin-antibody.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PA2109).<br> Gelsolin/GSN was detected in paraffin-embedded section of Human Intestinal Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gelsolin/GSN Antibody (PA2109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2109-5-IF-anti-gelsolin-antibody.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PA2109).<br> Gelsolin/GSN was detected in immunocytochemical section of NIH3T3 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Gelsolin/GSN Antibody (PA2109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2109-4-ihc-anti-gelsolin-antibody.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IHC analysis of Gelsolin/GSN using anti-Gelsolin/GSN antibody (PA2109).<br> Gelsolin/GSN was detected in paraffin-embedded section of Rat Cardiac Muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gelsolin/GSN Antibody (PA2109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2109-gsn-primary-antibodies-if-testing-6.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IF analysis of Gelsolin using anti-Gelsolin antibody (PA2109). <br> Gelsolin was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Gelsolin Antibody (PA2109) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2109-gsn-primary-antibodies-fcm-testing-7.pngAnti-Gelsolin/GSN Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-Gelsolin antibody (PA2109). <br>Overlay histogram showing U87 cells stained with PA2109 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gelsolin Antibody (PA2109, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-0-antibody-pa2110-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2110-1-WB-anti-aquaporin-0-antibody.jpgAnti-Aquaporin 0/MIP Antibody Picoband&reg;Anti-MIP antibody&#44; PA2110&#44; Western blotting<br>All lanes: Anti MIP (PA2110) at 0.5ug/ml<br>Lane 1: Mouse Spleen Tissue Lysate at 50ug<br>Lane 2: Mouse Intestine Tissue Lysate at 50ug<br>Predicted bind size: 28KD<br>Observed bind size: 50KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfkb-p105-p50-antibody-pa2111-boster.html2026-03-24T05:04:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2111-nfkb1-primary-antibodies-wb-testing-1.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg;Western blot analysis of NFkB p105/p50 using anti-NFkB p105/p50 antibody (PA2111). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human Daudi whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFkB p105/p50 antigen affinity purified polyclonal antibody (PA2111) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFkB p105/p50 at approximately 50, 120 kDa. The expected band size for NFkB p105/p50 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2111-nfkb1-primary-antibodies-ihc-testing-1.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg;IHC analysis of NFkB p105/p50 using anti-NFkB p105/p50 antibody (PA2111). <br> NFkB p105/p50 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFkB p105/p50 Antibody (PA2111) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2111-nfkb1-primary-antibodies-ihc-testing-2.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg;IHC analysis of NFkB p105/p50 using anti-NFkB p105/p50 antibody (PA2111). <br> NFkB p105/p50 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFkB p105/p50 Antibody (PA2111) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2111-nfkb1-primary-antibodies-ihc-testing-3.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg;IHC analysis of NFkB p105/p50 using anti-NFkB p105/p50 antibody (PA2111). <br> NFkB p105/p50 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFkB p105/p50 Antibody (PA2111) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfkb-p105-p50-antibody-pa2111-1-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2111-1-1-WB-anti-nfkb-p105-p50-antibody.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg;Anti-NFkB p105/p50 antibody&#44; PA2111-1&#44; Western blotting<br>All lanes: Anti NFkB p105/p50(PA2111-1) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 105KD<br>Observed bind size: 105KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-runx3-antibody-pa2112-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2112-1-WB-anti-runx3-cbfa3-antibody.jpgAnti-RUNX3 Antibody Picoband&reg;Anti-RUNX3 antibody&#44; PA2112&#44; Western blotting<br>All lanes: Anti RUNX3 (PA2112) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: Mouse Liver Tissue Lysate at 50ug<br>Predicted bind size: 44KD<br>Observed bind size: 44KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sidt1-antibody-pa2113-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2113-1-WB-anti-sidt1-sid-1-antibody.jpgAnti-SIDT1 Antibody Picoband&reg;Anti-SIDT1 antibody&#44; PA2113&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: COLO320 Cell Lysate<br>Lane 3: SW620 Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smad1-antibody-pa2114-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2114-1-WB-anti-smad1-antibody.jpgAnti-Smad1 Antibody Picoband&reg;Anti-Smad1 antibody&#44; PA2114&#44; Western blotting<br>Lane 1: SMMC Cell Lysate<br>Lane 2: K562 Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br>Lane 4: HELA Cell Lysate<br>Lane 5: JURKAT Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2114-2-IHC-anti-smad1-antibody.jpgAnti-Smad1 Antibody Picoband&reg;Anti-Smad1 antibody&#44; PA2114&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2114-3-IF-anti-smad1-antibody.jpgAnti-Smad1 Antibody Picoband&reg;Anti-Smad1 antibody&#44; PA2114&#44; ICC<br>ICC: A549 Cell https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smad5-antibody-pa2115-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2115-1-IHC-anti-smad5-antibody.jpgAnti-SMAD5 Antibody Picoband&reg;Anti-SMAD5 antibody&#44; PA2115&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2115-2-IHC-anti-smad5-antibody.jpgAnti-SMAD5 Antibody Picoband&reg;Anti-SMAD5 antibody&#44; PA2115&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2115-3-IHC-anti-smad5-antibody.jpgAnti-SMAD5 Antibody Picoband&reg;Anti-SMAD5 antibody&#44; PA2115&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2115-4-WB-anti-smad5-antibody.jpgAnti-SMAD5 Antibody Picoband&reg;Anti-SMAD5 antibody&#44; PA2115&#44; Western blotting<br>All lanes: Anti SMAD5 (PA2115) at 0.5ug/ml<br> Lane 1: K562 Whole Cell Lysate at 40ug<br> Lane 2: JURKAT Whole Cell Lysate at 40ug<br> Lane 3: PC 1-2 Whole Cell Lysate at 40ug<br> Lane 4: HELA Whole Cell Lysate at 40ug<br> Lane 5: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 52 KD<br> Observed bind size: 52KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nak-antibody-pa2117-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2117-1-IHC-anti-nak-antibody.jpgAnti-NAK/TBK1 Antibody Picoband&reg;Anti-NAK antibody&#44; PA2117&#44; IHC(P)<br>IHC(P): Rat Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2117-2-IHC-anti-nak-antibody.jpgAnti-NAK/TBK1 Antibody Picoband&reg;Anti-NAK antibody&#44; PA2117&#44; IHC(P)<br>IHC(P): Human Prostatic Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2117-3_1-WB-anti-nak-antibody.jpgAnti-NAK/TBK1 Antibody Picoband&reg;Anti-NAK antibody&#44; PA2117&#44; Western blotting<br>All lanes: Anti TBK1 (PA2117) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: Rat Testis Tissue Lysate at 50ug<br>Lane 3: Rat Liver Tissue Lysate at 50ug<br>Predicted bind size: 84KD<br>Observed bind size: 100KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt3a-antibody-pa2120-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2120-1-WB-anti-wnt3a-antibody.jpgAnti-Protein Wnt-3a Wnt3a Antibody Picoband&reg;Anti-WNT3A antibody&#44; PA2120&#44; Western blotting<br>All lanes: Anti WNT3A (PA2120) at 0.5ug/ml<br>WB: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 39KD<br>Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt4-antibody-pa2121-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2121-1-WB-anti-wnt4-antibody.jpgAnti-Protein Wnt-4 Wnt4 Antibody Picoband&reg;Anti-WNT4 antibody&#44; PA2121&#44; Western blotting<br>All lanes: Anti WNT4 (PA2121) at 0.5ug/ml<br>WB: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 37KD<br>Observed bind size: 37KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr9-antibody-pa2122-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2122-1-WB-anti-ccr9-antibody.jpgAnti-C-C chemokine receptor type 9 CCR9 Antibody Picoband&reg;Anti-CCR9 antibody&#44; PA2122&#44; Western blotting<br>All lanes: Anti CCR9 (PA2122) at 0.5ug/ml<br> Lane 1: JURKAT Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 42KD<br> Observed bind size: 42KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc5l-antibody-pa2123-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2123-1_1.jpgAnti-CDC5L Antibody Picoband&reg; Western blot analysis of CDC5L using anti-CDC5L antibody (PA2123). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates<br> Lane 2: human THP-1 whole cell lysates<br> Lane 3: rat C6 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC5L antigen affinity purified polyclonal antibody (Catalog # PA2123) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDC5L at approximately 100KD. The expected band size for CDC5L is at 92KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk6-antibody-pa2125-2-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2125-2-1-WB-anti-cdk6-antibody.jpgAnti-Cyclin-dependent kinase 6 Cdk6 Antibody Picoband&reg;Anti-Cdk6 antibody&#44; PA2125-2&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: Rat Lung Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk7-antibody-pa2126-1-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2126-1-1-WB-anti-cdk7-antibody.jpgAnti-Cyclin-dependent kinase 7 Cdk7 Antibody Picoband&reg;Anti-Cdk7 antibody&#44; PA2126-1&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: Rat Ovary Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2126-1-2-WB-anti-cdk7-antibody.jpgAnti-Cyclin-dependent kinase 7 Cdk7 Antibody Picoband&reg;Anti-Cdk7 antibody&#44; PA2126-1&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: A549 Cell Lysate<br>Lane 4: COLO320 Cell Lysate<br>Lane 5: JURKAT Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk7-antibody-pa2126-2-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2126-2-1-WB-anti-cdk7-antibody.jpgAnti-Cyclin-dependent kinase 7 Cdk7 Antibody Picoband&reg;Anti-Cdk7 antibody&#44; PA2126-2&#44; Western blotting<br>All lanes: Anti Cdk7 (PA2126-2) at 0.5ug/ml<br> Lane 1: Rat Testis Tissue Lysate at 50ug<br> Lane 2: Rat Ovary Tissue Lysate at 50ug<br> Lane 3: HELA Whole Cell Lysate at 40ug<br> Lane 4: MCF-7 Whole Cell Lysate at 40ug<br> Lane 5: A549 Whole Cell Lysate at 40ug<br> Lane 6: COLO320 Whole Cell Lysate at 40ug<br> Predicted bind size: 39KD<br> Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p2x6-antibody-pa2128-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2128-1-WB-anti-p2x6-antibody.jpgAnti-P2X6/P2RX6 Antibody Picoband&reg;Anti-P2X6 antibody&#44; PA2128&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: 22RV1 Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p2rx7-antibody-pa2129-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2129-1-WB-anti-p2rx7-p2x7-antibody.jpgAnti-P2X purinoceptor 7 P2RX7 Antibody Picoband&reg;Anti-P2RX7 antibody&#44; PA2129&#44; Western blotting<br>WB: U87 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pcsk9-antibody-pa2130-boster.html2026-03-24T05:04:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2130-pcsk9-primary-antibodies-wb-testing-1_1.jpgAnti-PCSK9 Antibody Picoband&reg;Western blot analysis of PCSK9 using anti-PCSK9 antibody (PA2130). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK9 antigen affinity purified polyclonal antibody (Catalog # PA2130) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PCSK9 at approximately 66 kDa. The expected band size for PCSK9 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2130-pcsk9-primary-antibodies-ihc-testing-2.jpgAnti-PCSK9 Antibody Picoband&reg; IHC analysis of PCSK9 using anti-PCSK9 antibody (PA2130). <br> PCSK9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PCSK9 Antibody (PA2130) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-plk2-antibody-pa2131-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2131-1-WB-anti-plk2-snk-antibody.jpgAnti-PLK2 Antibody Picoband&reg; Western blot analysis of PLK2/Snk using anti-PLK2/Snk antibody (PA2131). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: A431 Cell Lysate<br> Lane 2: 293T Cell Lysate<br> Lane 3: COLO-320 Cell Lysate<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLK2/Snk antigen affinity purified polyclonal antibody (Catalog # PA2131) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLK2/Snk at approximately 78KD. The expected band size for PLK2/Snk is at 77KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2131-2-IHC-anti-plk2-snk-antibody.jpgAnti-PLK2 Antibody Picoband&reg; IHC analysis of PLK2/Snk using anti-PLK2/Snk antibody (PA2131).<br> PLK2/Snk was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLK2/Snk Antibody (PA2131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2131-3-IHC-anti-plk2-snk-antibody.jpgAnti-PLK2 Antibody Picoband&reg; IHC analysis of PLK2/Snk using anti-PLK2/Snk antibody (PA2131).<br> PLK2/Snk was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLK2/Snk Antibody (PA2131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2131-4-IHC-anti-plk2-snk-antibody.jpgAnti-PLK2 Antibody Picoband&reg; IHC analysis of PLK2/Snk using anti-PLK2/Snk antibody (PA2131).<br> PLK2/Snk was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PLK2/Snk Antibody (PA2131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2131-plk2-primary-antibodies-fc-testing-5.jpgAnti-PLK2 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-PLK2 antibody (PA2131). <br>Overlay histogram showing A549 cells stained with PA2131 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLK2 Antibody (PA2131&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2131-plk2-primary-antibodies-icc-testing-6.jpgAnti-PLK2 Antibody Picoband&reg; ICC analysis of PLK2 using anti-PLK2 antibody (PA2131). <br> PLK2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-PLK2 Antibody (PA2131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-parathyroid-hormone-receptor-1-antibody-pa2132-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2132-1-WB-anti-parathyroid-hormone-receptor-1-antibody.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband&reg;Anti-Parathyroid Hormone Receptor 1 antibody&#44; PA2132&#44; Western blotting<br>All lanes: Anti Parathyroid Hormone Receptor 1 (PA2132) at 0.5ug/ml<br> Lane 1: SKOV Whole Cell Lysate at 40ug<br> Lane 2: U20S Whole Cell Lysate at 40ug<br> Lane 3: HELA Whole Cell Lysate at 40ug<br> Lane 4: SMMC Whole Cell Lysate at 40ug<br> Predicted bind size: 66KD<br> Observed bind size: 66KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2132-2-IHC-anti-parathyroid-hormone-receptor-1-antibody.jpgAnti-Parathyroid Hormone Receptor 1/PTH1R Antibody Picoband&reg;Anti-Parathyroid Hormone Receptor 1 antibody&#44; PA2132&#44; IHC(P)<br>IHC(P): Human Thyroid Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-syndecan-3-sdc3-antibody-pa2133-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2133-1-IHC-anti-syndecan-3-sdc3-antibody.jpgAnti-Syndecan 3/SDC3 Antibody Picoband&reg; IHC analysis of Syndecan 3/SDC3 using anti-Syndecan 3/SDC3 antibody (PA2133). <br> Syndecan 3/SDC3 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Syndecan 3/SDC3 Antibody (PA2133) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2133-2-IHC-anti-syndecan-3-sdc3-antibody.jpgAnti-Syndecan 3/SDC3 Antibody Picoband&reg; IHC analysis of Syndecan 3/SDC3 using anti-Syndecan 3/SDC3 antibody (PA2133). <br> Syndecan 3/SDC3 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Syndecan 3/SDC3 Antibody (PA2133) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2133-sdc3-primary-antibodies-wb-testing-3.jpgAnti-Syndecan 3/SDC3 Antibody Picoband&reg; Western blot analysis of Syndecan 3/SDC3 using anti-Syndecan 3/SDC3 antibody (PA2133). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U20S whole cell lysates, <br> Lane 2: rat PC-12 whole cell lysates, <br> Lane 3: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan 3/SDC3 antigen affinity purified polyclonal antibody (Catalog # PA2133) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan 3/SDC3 at approximately 60 kDa. The expected band size for Syndecan 3/SDC3 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tafazzin-taz-antibody-pa2135-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2135-taz-primary-antibodies-wb-testing-1.jpgAnti-Tafazzin/TAZ Antibody Picoband&reg;Western blot analysis of TAZ using anti-TAZ antibody (PA2135). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat skeletal muscle tissue lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: mouse heart tissue lysates,<br> Lane 5: mouse skeletal muscle tissue lysates,<br> Lane 6: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAZ antigen affinity purified polyclonal antibody (PA2135) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAZ at approximately 33 kDa. The expected band size for TAZ is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd134-ox40-antibody-pa2136-1-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2136-1-1-WB-anti-tnfrsf4-ox40-antibody.jpgAnti-CD134/OX40/TNFRSF4 Antibody Picoband&reg;Anti-CD134/OX40 antibody&#44; PA2136-1&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: HT1080 Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br>Lane 5: COLO320 Cell Lysate<br>Lane 6: MCF-7 Cell Lysate<br>Lane 7: SHC Cell Lysate<br>Lane 8: COLO320 Cell Lysate <br>Lane 9: SGC Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd134-ox40-antibody-pa2136-2-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2136-2-1-WB-anti-tnfrsf4-ox40-antibody.jpgAnti-CD134/OX40/TNFRSF4 Antibody Picoband&reg;Anti-CD134/OX40 antibody&#44; PA2136-2&#44; Western blotting<br>Lane 1: Mouse Brain Tissue Lysate<br>Lane 2: Mouse Spleen Tissue Lysate<br>Lane 3: Mouse Liver Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd134-ox40-antibody-pa2136-3-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2136-3-1-WB-anti-tnfrsf4-ox40-antibody.jpgAnti-CD134/OX40/TNFRSF4 Antibody Picoband&reg;Anti-CD134/OX40 antibody&#44; PA2136-3&#44; Western blotting<br>All lanes: Anti CD134/OX40 (PA2136-3) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Rat Liver Tissue Lysate at 50ug<br>Lane 3: Rat Kidney Tissue Lysate at 50ug<br>Predicted bind size: 29KD<br>Observed bind size: 29KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vcp-antibody-pa2137-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-vcp-primary-antibodies-wb-testing-1.jpgAnti-VCP Antibody Picoband&reg; Western blot analysis of VCP using anti-VCP antibody (PA2137). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: human K562 whole cell lysates,<br> Lane 6: human HepG2 whole cell lysates,<br> Lane 7: monkey COS-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # PA2137) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-vcp-primary-antibodies-wb-testing-2.jpgAnti-VCP Antibody Picoband&reg; Western blot analysis of VCP using anti-VCP antibody (PA2137). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat lung tissue lysates,<br> Lane 3: rat stomach tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse lung tissue lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # PA2137) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2137-3-IHC-anti-vcp-antibody.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PA2137). VCP was detected in paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PA2137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2137-4-IHC-anti-vcp-antibody.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PA2137). VCP was detected in paraffin-embedded section of Rat Cerebellum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PA2137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-5-ihc-anti-vcp-antibody.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PA2137). VCP was detected in paraffin-embedded section of Rat Epinephros tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PA2137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-6-ihc-anti-vcp-antibody.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PA2137). VCP was detected in paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PA2137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-7.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PA2137). <br> VCP was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PA2137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-8.jpgAnti-VCP Antibody Picoband&reg; ICC analysis of VCP using anti-VCP antibody (PA2137). <br> VCP was detected in immunocytochemical section of K562 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-VCP Antibody (PA2137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-9-ihc-anti-vcp-antibody.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PA2137). <br> VCP was detected in a frozen section of Rat Intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VCP Antibody (PA2137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-vcp-primary-antibodies-if-testing-10.jpgAnti-VCP Antibody Picoband&reg; IF analysis of VCP using anti- VCP antibody (PA2137). <br> VCP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-VCP Antibody (PA2137) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-vcp-primary-antibodies-fcm-testing-11.pngAnti-VCP Antibody Picoband&reg; Flow Cytometry analysis of HELA cells using anti-VCP antibody (PA2137). <br>Overlay histogram showing HELA cells stained with PA2137 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (PA2137, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-vcp-primary-antibodies-wb-testing-12.jpgAnti-VCP Antibody Picoband&reg; Western blot analysis of VCP using anti-VCP antibody (PA2137). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: human HepG2 whole cell lysates,<br> Lane 6: human K562 whole cell lysates,<br> Lane 7: human U87 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # PA2137) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 488 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2137-vcp-primary-antibodies-wb-testing-13.jpgAnti-VCP Antibody Picoband&reg; Western blot analysis of VCP using anti-VCP antibody (PA2137). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat lung tissue lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse lung tissue lysates,<br> Lane 7: mouse kidney tissue lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # PA2137) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 488 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc37-antibody-pa2139-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2139-1_1-WB-anti-cdc37-antibody.jpgAnti-Cdc37 Antibody Picoband&reg;Anti-Cdc37 antibody&#44; PA2139&#44; Western blotting<br>All lanes: Anti Cdc37(PA2139) at 0.5ug/ml<br>Lane 1: JURKAT Whole Cell Lysate at 40ug<br>Lane 2: 293T Whole Cell Lysate at 40ug<br>Predicted bind size: 44KD<br>Observed bind size: 44KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-i-antibody-pa2140-1-boster.html2026-03-25T05:21:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-col1a1-primary-antibodies-wb-review-1.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg; Western blot analysis of COL1A1 using anti-COL1A1 antibody (PA2140-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: cardiac tissue lysates from 3-month-old mice, <br> Lane 2: cardiac tissue lysates from 6-month-old mice, <br> Lane 3: cardiac tissue lysates from 12-month-old mice,<br> Lane 4: cardiac tissue lysates from 18-month-old mice.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for COL1A1 at approximately 138 kDa. The expected band size for COL1A1 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-bjbms-21-284-g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;RAL preserved the matrix of cartilage and bone and inhibited the overexpression of TGF-β1 and catabolic factors. Expression of aggrecan, Col-II, MMP-13, ADAMTS-5, Col-X, TGF-β1, and Col-I (scale bar, 100 μm). Col-II: Collagen type II; MMP-13: Matrix metalloproteinase-13; ADAMTS-5: A disintegrin and metalloproteinase with thrombospondin motifs-5; Col-X: Collagen type X; TGF-β1: Transforming growth factor-beta 1; Col-I: Collagen type I.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8112563/&orig_host=www.ncbi.nlm.nih.gov'>33259777</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13578_2022_789_fig6_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;TRPM8 inhibitor mitigates liver fibrosis in CCl 4 -treated mice. A H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in liver sections of CCl 4 -treated mice (n = 5 per group). Image J was used to quantify positively stained areas. Scale bars, 100 μm. B Serum levels of ALT and AST were measured in mice (n = 5 per group). C Expressions of α-SMA and COL1A1 were detected by immunoblotting (n = 3 per group). D Hepatic mRNAs of fibrogenic genes were measured by qRT-PCR assays in mice treated with M8-B or DMSO after CCl 4 induction (n = 5 per group). The results are expressed as mean ± SD. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-022-00789-4'>35525986</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13578_2022_789_fig2_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Liver fibrosis is attenuated in TRPM8 −/− mice after CCl 4 treatment. A Representative histology of H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in the liver of WT and TRPM8 −/− mice induced by CCl 4 (n = 5 per group). Positive staining areas were quantified by by Image J software. Scale bars, 100 μm. B Liver function was assessed by measuring the serum levels of ALT and AST in mice (n = 5 per group). C Immunoblotting analyses of α-SMA and COL1A1 expression in the liver (n = 3 per group). D Hepatic mRNA levels of fibrogenic genes were measured by qRT-PCR (n = 5 per group). The results are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-022-00789-4'>35525986</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fendo-13-846154-g004.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;MiR-619-5p is down-regulated and inhibits osteogenesis during osteogenic induction of hBMSCs. (A) The relative expression levels of miR-619-5p before and after osteogenic differentiation were determined by qRT-PCR. (B) Correlation analysis of LINC01485 and miR-619-5p expression levels during osteogenic differentiation. (C, D) The mRNA level of miR-619-5p in hBMSCs transfected with miR-199a-5p mimic (C) and miR-199a-5p inhibitor (D) by qRT-PCR. (E–I) Western blot analysis of the RUNX2 (E, F) , COL1A1 (E, G) , OSX (E, H) , and OPN (E, I) protein expression in hBMSCs transfected with miR-619-5p mimic, miRNA mimic NC, miR-619-5p inhibitor, and miRNA inhibitor NC after osteogenic induction and the corresponding gray value quantitative analysis. (J) ALP staining analysis of hBMSCs transfected with miR-619-5p mimic, miRNA mimic NC, miR-619-5p inhibitor, and miRNA inhibitor NC after osteogenic induction. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9161675/&orig_host=www.ncbi.nlm.nih.gov'>35663324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fendo-13-846154-g001.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;LNC01485 expression was up-regulated during osteogenic differentiation of hBMSCs. (A, C) ALP staining (A) and ALP activity assay (C) of hBMSCs before osteogenic induction and 7 days after induction. (B, D) hBMSCs were stained with Alizarin Red S (B) before osteogenic induction and at 14 days after induction, and the staining results were analyzed semi-quantitatively (D) . (E-I) The protein expression levels of RUNX2 (E, F) , COL1A1 (E, G) , OSX (E, H) , and OPN (E, I) level on Days 0, 7, 14, and 21 of osteogenic induction were detected by Western blot and quantified analysis by normalized to GAPDH. (J-O) The mRNA expression levels of RUNX2 (J) , OPN (K) , OCN (L) , COL1A1 (M) , OSX (N) , and LINC01485 (O) before and after osteogenic differentiation were determined by qRT-PCR. (P-R) Expression correlation analysis between LINC01485 and osteogenic genes RUNX2 (P) , OPN (Q) , and OCN (R) during osteogenic differentiation. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9161675/&orig_host=www.ncbi.nlm.nih.gov'>35663324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; Western blot analysis of COL1A1 using anti-COL1A1 antibody (PA2140-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HK2 whole cell lysates, <br> Lane 2: human HCC tissue lysates, <br> Lane 3: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL1A1 at approximately 180-200 kDa. The expected band size for COL1A1 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13062_2023_425_fig6_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Effect of KCNMA1-AS1 on osteogenic differentiation of hBMSCs with the SMAD9 signaling pathway activation. A and B- Protein levels of total SMAD9 and p-SMAD9 in lentivirus-transfected hBMSCs treated with DMSO or LDN193189 (100nM) detected by western blot after one week of osteogenic induction. GAPDH as the internal reference. C and D- The expression levels of COL1A1, RUNX2, OSX, and OPN in lentivirus-transfected hBMSCs treated with DMSO or LDN193189 (100nM) detected by western blot after one week of osteogenic induction. GAPDH as the reference. E and F- ALP staining ( E ) and ALP activity ( F ) in lentivirus-transfected hBMSCs treated with DMSO or LDN193189 (100nM) after one week of osteogenic induction. Scale bar of microscopical images, 100 μm. G and H- ARS staining ( G ) and ARS quantification ( H ) in lentivirus-transfected hBMSCs treated with DMSO or LDN193189 (100nM) after 14 days of osteogenic induction. Scale bar of microscopical images, 100 μm. **** P < 0.0001, compared with KCNMA1-AS1 + DMSO. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-023-00425-2'>38017487</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-2.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of COL1A1 using anti-COL1A1 antibody (PA2140-1). <br> COL1A1 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PA2140-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-gr7c.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Verification of Hub Genes (A).Barplot of mRNA expression levels of THBS2 between IPF and control group. (B).Barplot of mRNA expression levels of THBS2 between RA-UIP and control group. (C).Barplot of mRNA expression levels of TIMP1 between IPF and control group. (D).Barplot of mRNA expression levels of TIMP1 between RA-UIP and control group. (E).Barplot of mRNA expression levels of POSTN between IPF and control group. (F).Barplot of mRNA expression levels of POSTN between RA-UIP and control group. (G).Barplot of mRNA expression levels of CD19 between the IPF and control group. (H).Barplot of mRNA expression levels of CD19 between RA-UIP and control group. (I).Barplot of mRNA expression levels of α-SMA, COL1A1, and hub genes between TGF-β1-stimulated fibroblast group and control group. (J).Images of H&E staining (H&Ex200) and immunohistochemical staining (DABx200) of bleomycin-induced mouse group and control group. (K).Scatter plots of positive immunostained area proportions of α-SMA, COL1A1, and hub genes between bleomycin-induced mouse group and control group. (L).Boxplot of mRNA expression levels of hub genes between IPF and control group on dataset GSE150910. (M).Boxplot of mRNA expression levels of hub genes between RA and control group on dataset GSE89408. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/heliyon/fulltext/S2405-8440(24)04119-7'>38571583</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-3.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of COL1A1 using anti-COL1A1 antibody (PA2140-1). <br> COL1A1 was detected in a paraffin-embedded section of human gastric carcinomaa tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PA2140-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-12967_2024_5466_fig2_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Increased expression of HIF-1α and ANGPTL4 in CKD rats. A Immunohistochemical staining to assess the expression of HIF-1α, ANGPTL4, α-SMA, and Col-I in the renal tissues of the two groups (× 200 magnification, scale bar = 50 µm). B – E Quantification of immunohistochemical staining (n = 5/group). F – I qRT‒PCR analysis of the expression of Hif-1α , Angptl4 , α-sma , and Col-I mRNA in the renal tissues of the two groups of rats. All the data are presented as the mean ± standard deviation; * P < 0.05, ** P < 0.01 vs. the corresponding control group at the same time point <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05466-3'>38992710</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-4_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of COL1A1 using anti-COL1A1 antibody (PA2140-1). <br> COL1A1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PA2140-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13287_2024_4095_fig4_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;hAMSCs ameliorate fibrosis by promoting epithelial cell proliferation, reducing epithelial cell apoptosis, and attenuating epithelial mesenchymal transition through paracrine effects. A Morphological changes of cells (scale bar = 100 μm). B Cellular epithelial mesenchymal transition, collagen production indices E-CAD, Cytokeratin, COL1A1, N-CAD, VIMENTIN, and ZO −1 mRNA expression levels. C N-CAD, E-CAD protein expression levels. D Morphological changes of cells after co-culture (scale bar = 100 μm). E mRNA expression levels of COL1A1, E-CAD, N-CAD, and TGFBR1, which are related to EMT. F Schematic illustration of the timeline of the cellular experiments with the morphology of A549 cells in the Transwells of the experimental groups. F Time axis of cell experiments and morphology of A549 cells and hAMSCs in Transwell (scale bar = 200 μm). G EdU staining and the proportion of EdU-positive cells (scale bar = 311.3 μm). H PCNA immunofluorescence staining and the proportion of PCNA-positive cells (scale bar = 311.3 μm). I Results of Annexin-FITC/PI staining and statistical graphs. J BAX immunofluorescence staining results (scale bar = 311.3 μm). K BAX mRNA expression level. L BAX protein expression level. M COL1A1 and N-CAD immunofluorescence staining results (scale bar = 311.3 μm). N COL1A1 and N-CAD mRNA expression level. O N-CAD protein expression level. P TGFBR1 mRNA expression level. Q TGFBR1 protein expression level. Data are presented as the (‾x ± s, n = 3). ns: P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-024-04095-3'>39757225</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13287_2024_4095_fig3_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Effects of hAMSCs with different hsa-miR181a-5p expression levels on lung fibrosis mice. A timeline of animal experiments. B Schematic diagram of the position of modeling cannula. C In vivo imaging results of mice 7 days after injection of hAMSCs. D Changes in body weight of mice. E General condition and appearance of lung tissues of mice in each experimental group. F Lung index of mice after sampling. G %Area(collagen volume fraction):Percentage of positive area of collagen blue staining of lung tissue sections using Masson staining. H HE and MASSON staining, expression of the Alpha-SMA marker, and the collagen marker COL1A1 in lung tissue sections from mice in each experimental group (scale = 50 μm or 100 μm). I N-CAD immunofluorescence staining results in lung tissue sections fron mice in each experimental group (scale = 155.6 μm). J N-CAD, E-CAD, VIMENTIN, and TGFBR1 protein expression levels. Data are presented as the (‾x ± s, n = 4). ns: P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-024-04095-3'>39757225</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-41467_2025_56872_fig9_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;In situ dural mater regeneration enhanced by Janus SIS in a rat spinal dural defect model. a Experimental design include MRI, histological analysis and immunology evaluations. b Macroscopic photographs of spinal tissues at 8 weeks after operation, including the normal control (without injury), control group, SIS group and Janus SIS group. c , d Histological evaluation (H&E and Masson’s trichrome staining) of spinal dural mater repair in different groups. The blue arrow indicates the normal spinal dura mater. The red arrow indicates newborn dura mater-like tissue. The green arrow indicates fibrotic tissue. The black arrow indicates residual SFMA microgroove coating. e Immunohistochemical staining of α-SMA and collagen I at the focal area of epidural tissues in different groups. f Quantitative analysis of newborn collagen tissue in the defect area. g , h Quantitative analysis of α-SMA positive cells and collagen I expression in histological sections. One-way analysis of variance (ANOVA) with a Tukey’s post hoc test was used for multiple comparisons. Values in ( f , g , and h ) represent the mean ± SD (three independent replicates ( n = 3)). Source data and exact P -values are provided as a source data file. (* P < 0.05, ** P < 0.01, *** P < 0.001). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-56872-0'>39955276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-41467_2025_56872_fig6_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;The morphology, proliferation, and collagen I expression of fibroblasts cultured on silk-based coatings. a , b CLSM and SEM images of the cytoskeleton and morphology of NIH3T3 fibroblasts on the unmodified SIS, SFMA-SIS, SFMA microgrooved surface, and HAMA-SilMA surface. c Immunofluorescence staining of collagen I secreted by fibroblasts on the unmodified SIS, SFMA-SIS, and SFMA microgrooved surfaces. d Cell proliferation analysis on different surfaces using the CCK-8 Kit. Values in ( d ) represent the mean ± SD (three independent replicates ( n = 3)). One-way analysis of variance (ANOVA) with a Tukey’s post hoc test was used for multiple comparisons. Source data and exact P -values are provided as a source data file. (* P < 0.05, ** P < 0.01, *** P < 0.001). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-56872-0'>39955276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-images_medium_s1793545824420021figf6.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;IF staining images of (a) BMP-2, (b) COL-1, (c) OCN, and (d) OPN in BMSCs (red: osteogenic proteins and genes; green: cytoskeleton; blue: nucleus). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.worldscientific.com/doi/abs/10.1142/S1793545824420021'>10.1142/S1793545824420021</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fmolb-07-00199-g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;GCLC was dramatically decreased in liver tissues of patients with HCV-related liver fibrosis. Liver tissues from healthy liver transplant donor ( n = 5) and patients with HCV-related liver fibrosis ( n = 8) were detected. (A) Liver fibrosis was evaluated with Masson staining, and expression of α-SMA, COL1, and GCLC were detected by IHC. Positive staining was indicated by brown color. (B–D) Expression of α-SMA, COL1, and GCLC in liver tissues of HCV patients was detected by qRT-PCR (B) and Western blot (C,D) . (E–G) Expression of ER stress-related genes in liver tissues of HCV patients was detected by qRT-PCR (E) and Western blot (F,G) . (H–J) Expression of inflammatory factor NF-κB, IKKB, and TNFα in liver tissues of HCV patients was detected by qRT-PCR (H) and Western blot (I,J) . (K–M) Expression of fibrotic genes such as TGFβ1, MMP2, and TIMP1 in liver tissues of HCV patients was detected by qRT-PCR (K) and Western blot (L,M) . * P < 0.05, ** P < 0.01. HC: healthy control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2020.00199/full'>33015132</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fmolb-07-00199-g004.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Overexpression of GCLC could suppress the activation of HSC. (A–C) Expression of GCLC, α-SMA, COL1, TGFβ1, MMP2, and TIMP1 in LX-2 cells transfected by puc19, puc19-hGCLC, and GCLC-expressing LX-2 cells treated with L-BSO was detected by qRT-PCR (A) and Western blot (B,C) . (D–F) The level of total GSH (D) , GSH (E) , and 2GSH/GSSG ratio (F) was increased in LX-2 cells transfected with the puc19-hGCLC plasmid. (G–H) ROS-DCF was dramatically decreased in LX-2 cells transfected with the puc19-hGCLC plasmid. (I–K) Expression of ER stress-related gene GRP78, CHOP, and IRE1 in LX-2 cells transfected with puc19 and puc19-hGCLC plasmid was detected by qRT-PCR (I) and Western blot (J,K) . (L–N) Expression of inflammatory factors NF-κB, IKKB, and TNFα mRNA and protein was detected by qRT-PCR (L) and Western blot (M,N) . * P < 0.05, ** P < 0.01; # P < 0.05, ## P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2020.00199/full'>33015132</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-12967_2024_5466_fig5_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;The HIF-1α-ANGPTL4 pathway is activated in hypoxia-induced HK2 cell fibrosis. After 24 h of hypoxia induction in HK2 cells, an in vivo model of RIF induction was established. A Western blot analysis was conducted to assess the protein levels of HIF-1α, ANGPTL4, α-SMA, and Col-I, and the protein levels were quantified using ImageJ software (n = 3/group) ( B – E ). F – I qRT‒PCR was used to measure the mRNA expression of HIF-1α , ANGPTL4 , α-SMA , and COL-I in each group. J – L Immunofluorescence staining for HIF-1α and ANGPTL4 (× 200 magnification, scale bar = 100 µm). All the data are presented as the mean ± standard deviation. * P < 0.05, ** P < 0.01 vs. the control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05466-3'>38992710</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-41467_2025_63152_fig9_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Panx1 knockout mitigates renal fibrosis in a murine model of unilateral ischemia‒reperfusion injury. a Relative mRNA levels of Collagen I , Fibronectin , and αSMA in the renal tissue. b , c Representative immunoblots and quantification of Collagen I and α-SMA in renal tissue. Representative images and quantification of d , e Sirius Red, f Collagen I, and g Fibronectin staining in renal tissue. Scale bars, 50 µm (Sirius Red); 20 µm (Collagen I, Fibronectin). For all panels, data are from WT Sham (n = 6), K/O Sham (n = 6), WT d14-IRI (n = 8), K/O d14-IRI (n = 7), WT d28-IRI (n = 8), K/O d28-IRI (n = 8) mice. Data are presented as means ± SD. P values were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Exact P values are provided in the Source Data file. *P < 0.05, **P < 0.01, ***P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-63152-4'>40825974</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-12967_2024_5466_fig6_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Impact of HIF-1α inhibitors on the levels of ANGPTL4 and fibrosis. HK2 cells were subjected to 2-MeOE2 (5 μmol/L) pretreatment for 12 h, followed by 24 h of hypoxia. A Western blot analysis was used to assess the protein levels of HIF-1α, ANGPTL4, α-SMA, and Col-I, and the protein levels were quantified using ImageJ software (n = 3/group) ( B – E) . F – I qRT‒PCR was utilized to evaluate the mRNA expression of HIF-1α , ANGPTL4 , α-SMA , and COL-I in each group. All the data are presented as the mean ± standard deviation. * P < 0.05, ** P < 0.01 vs. the control group; # P < 0.05, ## P < 0.01 vs. the model group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05466-3'>38992710</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-12967_2024_5466_fig7_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Reducing or overexpressing ANGPTL4 affects HK2 cell fibrosis. HK2 cells were transfected with ANGPTL4 siRNA for 24 h, followed by hypoxia-induced fibrosis for an additional 24 h. A Western blotting analysis was conducted to assess the protein levels of ANGPTL4, HIF-1α, α-SMA, and Col-I, and the protein levels were quantified using ImageJ software (n = 3/group) ( B – E ). F – I qRT‒PCR was used to evaluate the mRNA levels of ANGPTL4 , HIF-1α , α-SMA , and COL-I in each group. HK2 cells were transfected with the ANGPTL4 plasmid for 48 h. J Western blotting analysis was used to evaluate the protein levels of ANGPTL4, HIF-1α, α-SMA, and Col-I, and quantitative analysis was carried out using ImageJ software ( K – N ). O – R qRT‒PCR was utilized to examine the mRNA expression of ANGPTL4 , HIF-1α , α-SMA , and COL-I in each group. All the data are presented as the mean ± standard deviation. * P < 0.05, ** P < 0.01 compared to the control group; # P < 0.05, ## P < 0.01 compared to the scrambled siRNA ( +) or pcDNA3.1 ( +) group; ns: not significant <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-024-05466-3'>38992710</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fendo-13-846154-g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;LINC01485 regulates osteogenic differentiation of hBMSCs. (A, B) The overexpression (A) and interference (B) efficiency of LINC01485 was determined by qRT-PCR in hBMSCs after transduction with LV-LINC01485 and sh-LINC01485. (C–F) The mRNA levels of RUNX2 (C) , COL1A1 (D) , OSX (E) , and OCN (F) after 14 days of osteogenic induction in hBMSC infected with lentivirus by qRT-PCR. (G, H) Western blot analysis of the RUNX2 (G, I) , COL1A1 (G, I) , OSX (G, J) , and OPN (G, K) protein expression in hBMSCs infected with lentivirus after osteogenic induction 14 days later and the corresponding gray value quantitative analysis. (L, M) ALP staining (L) and ALP activity (M) of hBMSC cells infected with lentivirus after 7 days of osteogenic induction. (N, O) Alizarin Red S staining (N) and semi-quantitative analysis (O) of infected hBMSCs with lentivirus after 14 days osteogenic induction. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9161675/&orig_host=www.ncbi.nlm.nih.gov'>35663324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-ijbsv20p2310g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Wnt upregulation is associated with macrophages polarization and activation in vitro . ( A ) Characterization of the isolated bone marrow-derived macrophage (BMDM). Immunofluorescence staining for F4/80 showed the purity of cultured BMDMs. Scale bar, 25 µm. ( B ) BMDMs were induced to M1 polarization using of IFN-γ and LPS, and the expressions of various Wnts were detected by qRT-PCR. ( C ) BMDMs were induced to M2 polarization by IL-4, and the expressions of various Wnts were detected by qRT-PCR. ( D ) Western blot analyses showed that Wnts mixture induced M1 polarization by upregulating CD86 and TNF-α. ( E - F ) Quantitative data of CD86 and TNF-α in different groups as indicated. * P < 0.05 versus controls (n=3). ( G - I ) qRT-PCR showed that Wnts induced the mRNA expression of M1 polarization markers such as iNOS, IL-1β and CD86. ( J ) Western blotting showed that Wnts induced M2 polarization markers mannose receptor (MR) and arginase-1. ( K , L ) Quantitative data of MR and arginase 1 proteins. * P < 0.05 versus controls (n=3). ( M , N ) qRT-PCR showed that Wnts induced the mRNA expression of MR and TGF-β1. ( O ) Western blotting showed that Wnts induced fibrosis-related proteins such as fibronectin, collagen I and α-SMA in BMDMs. ( P ) Quantitative data of fibronectin (FN), collagen I (Col I) and α-smooth muscle actin (α-SMA) proteins. * P < 0.05 versus controls (n=3). ( Q ) qRT-PCR showed that Wnts induced the mRNA expression of FN, Col I and α-SMA. * P < 0.05 versus controls (n=3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11008274/&orig_host=www.ncbi.nlm.nih.gov'>38617540</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fendo-13-846154-g006.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;LINC01485 acts as a ceRNA of miR-619-5p to regulate RUNX2 and osteogenic differentiation. (A–E) Western blot analysis of the RUNX2 (A, B) , COL1A1 (A, C) , OSX (A, D) , and OPN (A, E) protein expression in hBMSCs infected with sh-NC or sh-LINC01485 lentivirus along with miRNA inhibitor NC or miR-619-5p inhibitor after osteogenic induction and the corresponding gray value quantitative analysis. (F) ALP staining analysis of hBMSCs infected with sh-NC or sh-LINC01485 lentivirus along with miRNA inhibitor NC or miR-619-5p inhibitor after osteogenic induction. (G) ALP staining analysis of hBMSCs infected with LV-NC or LV-LINC01485 lentivirus along with miRNA mimic NC or miR-619-5p mimic after osteogenic induction. (H) Luciferase activity of RUNX2-WT upon transfection of pcDNA3.1, pcDNA3.1-LINC01485, or miR-619-5p mimic into HEK293T cells. ns, none significance. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9161675/&orig_host=www.ncbi.nlm.nih.gov'>35663324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-40164_2023_471_fig5_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;FAK inhibitor decreased collagen deposition and inhibited CAF activation. A Diagram depicting treated time schedule with FAK inhibitors for the subcutaneous KL tumor model. B IHC staining for p-FAK in KL tumors treated with FAK inhibitor at different time points (Day 3, 7,14). Scale bars, 200 μm. C GO analysis for significantly downregulated genes in KL tumors treated with FAK inhibitors at Day14 compared to control (left) and treated with FAK inhibitors at Day14 compared to Day7. Representative IHC or immunofluorescent staining and quantification of D Collagen I, scale bars, 200 μm. E Collagen III, scale bars, 200 μm. F FAP, scale bars, 50 μm. G α-SMA, scale bars, 50 μm, in KL tumors treated with FAK inhibitor at different time points (Day 3, 7,14). Red, FAP staining; green, α-SMA staining; blue, DAPI staining. Unpaired Student’s t -test was performed and results in each group were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ns p-values with no statistical difference. KL, KRAS G12D LKB1 −/− ; CAF, cancer-associated fibroblast; FAK, focal adhesion kinase; FAP, fibroblast activation protein; SMA, smooth muscle actin; IHC, immunohistochemistry; BP, biological process; CC, cellular component; MF, molecular function; SEM, standard error of mean <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40164-023-00471-6'>38291516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13578_2022_789_fig3_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;TRPM8 deficiency alleviates BDL-induced liver fibrosis in mice. A Representative images of H&E, Sirius Red, Masson’s trichrome, and IHC staining for α-SMA and COL1A1 in the liver of WT and TRPM8 −/− mice operated with BDL (n = 5 per group). Positive staining areas were quantified by Image J software. Scale bars, 100 μm. B Liver function was assessed by measuring the serum levels of ALT and AST in mice (n = 5 per group). C Immunoblotting analyses of α-SMA and COL1A1 expression in the liver (n = 3 per group). D Hepatic mRNA levels of fibrogenic genes were measured by qRT-PCR (n = 5 per group). The results are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-022-00789-4'>35525986</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-40164_2023_471_fig3_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;KL tumors had excessive collagen deposition and collagen blocks T cell infiltration into tumor nest. A GO analysis for significantly upregulated genes in KL tumors compared to KP tumors. Representative and related GO signal pathways among top 30 upregulated pathways were listed here. B Reactome analysis for significantly upregulated genes in KL tumors compared to KP tumors. Representative and related Reactome signal pathways among top 20 upregulated pathways were listed here. C GSEA analysis for ECM-receptor interaction and GAP junction in KL and KP tumors. D Heatmap of RNA-seq showing collagen-related genes statistically significant (FDR < 0.05) differentially expressed in KL subcutaneous tumors. E Representative images and quantification of area performed by Masson, Siruis Red, Collagen I and Collagen III. Unpaired Student’s t-test was used to compare the statistical significance between two groups. Scale bars, 100 μm. inset scale bars, 50 μm. F Representative images showing spatial relations between collagen deposition and CD8 + TILs infiltration. Scale bars, 100 μm. inset scale bars, 20 μm. G Representative images and quantification of CD8 + TILs infiltrated in tumor nest and stroma in two groups. Scale bars, 100 μm. Results in each group were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ns p-values with no statistical difference. KL, KRAS G12D LKB1 −/− ; KP, KRAS G12D TP53 −/− ; BP, biological process; CC, cellular component; MF, molecular function; ECM, extracelluar matrix; TIL, tumor infiltrating lymphocyte; SEM, standard error of mean <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40164-023-00471-6'>38291516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fcell-09-748804-g001.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Characterization and trilineage differential potency of SMSCs from osteoarthritis (OA) keen synovium. (A) Surface marker expression of synovial mesenchymal stem cells (SMSCs). Results showed negativity for hematopoietic marker (CD34) and macrophage marker (CD68) and showed positivity for mesenchymal stem cell (MSC) markers like CD105 and CD90. (B) Multi-directional differentiation of SMSCs; chondrogenesis was accessed by safranin O staining (SO), osteogenesis was accessed by alizarin red S staining (ARS), and adipogenesis was accessed by Oil red O staining. (C) The EdU staining of SMSCs after the treatment of different concentrations of nocodazole (N) (5, 10, 25, 50, 100 nM) and docetaxel (D) (0.5, 1, 2.5, 5, 10 nM) in chondrogenic medium (CM) for 1 week. Scale bar, 100 μm. (D) Quantification of the data of (C) . n = 5. (E) The cell viability of SMSCs after the treatment of different concentrations of nocodazole (N) (5, 10, 25, 50, 100 nM) and docetaxel (D) (0.5, 1, 2.5, 5, 10 nM) in chondrogenic medium (CM) for 1 week. (F) Crystal violet staining of SMSCs after the treatment of different concentrations of nocodazole (N) (5, 10, 25, 50, 100 nM) and docetaxel (D) (0.5, 1, 2.5, 5, 10 nM) in chondrogenic medium (CM) for 1 week. Scale bar, 100 μm. (G) RT-qPCR analyses of SOX9 , COL2A1 , ACAN , RUNX2 , COL1A1 , and COL10A1 in SMSCs treated with nocodazole (N) and docetaxel (D) in chondrogenic medium (CM) for 1 week. 2.5 nM of docetaxel had significant effects on chondrogenesis in SMSCs. Data are represented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.748804/full'>34746145</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-40164_2023_471_fig8_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Impact of combining FAK inhibitor and PD-1 blockade on TME in KL mouse model. A Representative IHC staining of collagen I and quantification of collagen I staining area in different treatment schedule shown in Fig. A). Scale bars, 200 μm. B Representative IF staining of α-SMA and quantification of α-SMA staining area in different treatment schedule shown in Fig. A). Scale bars, 100 μm. C Representative IF staining of FAP and quantification of FAP staining area in different treatment schedule shown in Fig. A). Scale bars, 100 μm. D Quantification of CD8 + TILs and ratio of CD8 + T cells to Treg in each group. E Representative CD8 IHC staining and quantification of intratumoral primary tumor regions in each group. Scale bars, 100 μm. F Representative CD8 IHC staining and quantification of metastatic lung tumor regions in each group. Scale bars, 100 μm. Inset scale bars, 50 μm. Unpaired Student’s t -test was performed and results in each group were presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, and ns p-values with no statistical difference. FAK, focal adhesion kinase; PD-1, programmed death-1; TME, tumor microenvironment; IHC, immunohistochemistry; IF, immunofluorescence; FAP, fibroblast activation protein; TIL, tumor infiltrating lymphocyte; Treg, regulatory T cell; SEM, standard error of mean <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40164-023-00471-6'>38291516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-bjbms-21-284-g003.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Statistical analysis of the results of immunohistochemistry. The quantified protein levels are listed as follows (IOD/mm 2 ): (A) Aggrecan; (B) Col-II; (C) MMP-13; (D) ADAMTS-5; (E) Col-X; (F) TGF-β1; and (G) Col-I. ^ p < 0.05 versus the ACLT + OVX group;* p < 0.05 versus the sham group. Col-II: Collagen type II; MMP-13: Matrix metalloproteinase-13; ADAMTS-5: A disintegrin and metalloproteinase with thrombospondin motifs-5; Col-X: Collagen type X; TGF-β1: Transforming growth factor-beta 1; Col-I: Collagen type I; IOD: Integrated optical density.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8112563/&orig_host=www.ncbi.nlm.nih.gov'>33259777</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-40164_2023_471_fig4_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Activated CAF were abundant in KL tumors and fibroblastic FAK was hyperactivated. A The correlation between CAF infiltration and COL1A1 or COL3A1 via TIMER2.0. B The correlation between markers of activated CAF (FAP or α-SMA) and COL1A1 or COL3A1 via TIMER2.0. C Computed method calculating infiltrating levels of CAF in KP and KL subcutaneous tumors (n = 3 per each group) [ ]. D Expression levels of FAP in L929 cells, after co-cultured with KL or KP tumors cells for 72 h by RT-qPCR. The gene expression data of 72 h treated L929 cells were normalized to the DMEM control group. Additional TGF-β was used as positive control for activating CAF. Data represents mean ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, and ns represents p-values with no statistical difference. E Representative immunofluorescent staining of sections from KL or KP tumors. Red, FAP staining; green, α-SMA staining; blue, DAPI staining. Scale bars, 200 μm. magnified scale bars,100 μm. F KEGG analysis for significantly upregulated genes in KL tumors compared to KP tumors. Representative and related KEGG signal pathways among top 30 upregulated pathways were listed here. G Immunofluorescence staining and IHC staining co-localizing CAF and activated FAK. Scale bars, 100 μm. magnified scale bars, 20 μm. KL, KRAS G12D LKB1 −/− ; KP, KRAS G12D TP53 −/− ; CAF, cancer-associated fibroblast; FAK, focal adhesion kinase; FAP, fibroblast activation protein; SMA, smooth muscle actin; IHC, immunohistochemistry; TGF-β, transforming growth factor-beta; DMEM, dulbecco's modified eagle's medium; SEM, standard error of mean <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40164-023-00471-6'>38291516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-fmolb-07-00199-g003.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;GCLC was decreased in activated HSC induced by condition medium from LO2-CORE and HepG2-CORE. (A1–C1) Expression of α-SMA, COL1, and GCLC in activated HSC induced by CM from LO2-CORE was detected by qRT-PCR (A1) and Western blot (B1,C1) . (A2–C2) Expression of ER stress-related genes GRP78, CHOP, and IRE1 in activated HSC was detected by qRT-PCR (A2) and Western blot (B2,C2) . (A3–C3) Expression of inflammatory factor NF-κB, IKKB, and TNFα in activated HSC was detected by qRT-PCR (A3) and Western blot (B3,C3) . (A4–C4) Expression of fibrotic genes such as TGFβ1, MMP2, and TIMP1 in activated HSC was detected by qRT-PCR (A4) and Western blot (B4,C4) . (D1–F1) Expression of α-SMA, COL1, and GCLC in activated HSC induced by CM from HepG2-CORE was detected by qRT-PCR (D1) and Western blot (E1,F1) . (D2–F2) Expression of ER stress-related genes in activated HSC was detected by qRT-PCR (D2) and Western blot (E2,F2) . (D3–F3) Expression of inflammatory factors in activated HSC was detected by qRT-PCR (D3) and Western blot (E3,F3) . (D4–F4) Expression of fibrotic genes in activated HSC was detected by qRT-PCR (D4) and Western blot (E4,F4) . * P < 0.05, ** P < 0.01. CM: conditioned medium.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/molecular-biosciences/articles/10.3389/fmolb.2020.00199/full'>33015132</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13062_2023_425_fig1_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;KCNMA1-AS1 is upregulated when hBMSCs undergo osteogenic differentiation. A- Differentially expressed lncRNAs analyzed by qPCR after 14 days of osteogenic induction. B- Relative expression levels of KCNMA1-AS1 were measured using qPCR, and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used for normalization. C-F- Relative mRNA levels of COL1A1 ( C ), RUNX2 ( D ), OSX ( E ), and OPN ( F ) measured through qPCR, normalized to GAPDH. G and H- The protein levels of COL1A1, RUNX2, OSX, and OPN were detected by western blot. The internal reference is GAPDH. I and J- ALP staining ( I ) and ALP activity ( J ). hBMSCs cultured in osteogenic medium (OM) or growth medium (GM) for a week. Scale bar of microscopic images, 100 μm. K and L- ARS staining ( K ) and ARS quantification ( L ). hBMSCs were grown in an osteogenic medium (OM) or growth medium (GM) for two weeks. Scale bar of microscopical images, 100 μm. M-O- Correlation of the expression of KCNMA1-AS1 with that of COL1A1 ( M ), RUNX2 ( N ), and OSX ( O ) during osteogenic differentiation. ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, compared with 0 day/GM. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-023-00425-2'>38017487</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-41598_2017_8334_fig9_html.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Ang II via NF- κ B and Smad pathways increases CTGF production and CTGF is involved in Ang II-induced ECM accumulation in LX-2 cells. Serum-starved LX-2 cells were preincubated for 0.5 h with BAY11-7082 (a specific NF- κ B inhibitor; 10 −6 M), or SIS3 (a specific Smad3 inhibitor, 10 −6 M) alone or simultaneously in the presence or absence of Ang II (10 −7 M) stimulation for 4 h (to detect CTGF) or 24 h (to detect type I collagen and fibronectin). Whole cell lysates were immunoblotted with antibodies against CTGF, type I collagen and fibronectin, respectively. β -Actin level served as a control for equal protein loading. ( A ) Representative immunoblot bands are shown for the indicated antibodies. ( B,C ) The histogram represents results of the densitometric scans for the protein bands of CTGF, type I collagen and fibronectin after normalization with β -actin. ( D ) CTGF concentrations in the cell supernatants were determined by ELISA, and the value in the vehicle control group was defined as 1.0. ( E ) Serum-starved LX-2 cells transiently transfected with scrambled siRNA or CTGF siRNA were treated with Ang II (10 −7 M) for 4 h (to detect CTGF) or 24 h (to detect type I collagen and fibronectin), respectively. After treatment, aliquots of whole cell lysates were subjected to immunoblotting with specific antibodies as indicated. β -Actin was used as an internal control. ( E ) The experiments were repeated thrice with similar results and representative immunoblot bands for CTGF, type I collagen and fibronectin are shown. ( F,G ) Fold-change in relative protein level of each protein is shown after normalizing with β -actin. All data are presented as mean ± SD of 3 independent experiments. # P < 0.05 versus vehicle- or scrambled siRNA-treated control cells; * P < 0.05 versus Ang II- or Ang II + scrambled siRNA-treated cells. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-08334-x'>28798388</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13062_2023_425_fig2_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;KCNMA1-AS1 promotes osteogenic differentiation of hBMSCs in vitro. A- Transfection efficiency of KCNMA1-AS1 overexpression and KCNMA1-AS1 knockdown was measured by qPCR, normalized to GAPDH. B-E- Relative mRNA levels of COL1A1 ( B ), RUNX2 ( C ), OSX ( D ), and OPN ( E ) in hBMSCs transfected with lentivirus measured by qPCR after one week of osteogenic induction, normalized to GAPDH. F and G- The protein levels of COLA1, RUNX2, OSX, and OPN in hBMSCs transfected with lentivirus detected by western blot after one week of osteogenic induction. GAPDH was used as the internal reference. H and I ALP staining ( H ) and ALP activity ( I ) in hBMSCs transfected with lentivirus after one week of osteogenic induction. Scale bar of microscopical images, 100 μm. J and K- ARS staining ( J ) and ARS quantification ( K ) in hBMSCs transfected with lentivirus after two weeks of osteogenic induction. Scale bar of microscopical images, 100 μm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, in comparison to NC/sh-NC. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-023-00425-2'>38017487</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-irnf_a_2387429_f0001_c.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Kidney injury caused by hyperuricemia (HUA) in UNx mice. (A) The flowchart of the animal experiment. (B–D) Alteration of serum creatinine(scr), urea (SUN), and uric acid (SUA) in three groups of mice. The data are expressed as mean ± SEM (n = 6). (E) Representative images (40×) for hematoxylin and eosin(H&E) staining of kidney tissues and score of renal injury; green arrow points to renal interstitial inflammatory changes, the black arrow points to vacuolization and atrophy of the renal tubules. (F) Immunohistochemical staining of α-smooth muscle actin(α-SMA) in kidney tissues and percentage of α-SMA positive area. (G) immunohistochemical staining of collagen I in kidney tissues and percentage of collagen I positive area. (H-I) Concentrations of malondialdehyde(MDA) and superoxide dismutase(SOD) in kidney tissue; (J–L) Western blotting detection of expression of microtubule-associated protein 1 light chain 3 I/II(LC3I/II) and Beclin I in kidney tissue and histogram analysis. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001. Analysis was performed by ANOVA followed by Tukey’s multiple comparison test. Sham, sham operations group; UNx, unilateral nephrectomy group; UNx + HPD, unilateral nephrectomy + adenine and potassium oxinate diet. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.1080/0886022X.2024.2387429'>39132829</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-13062_2023_425_fig3_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;KCNMA1-AS1 promotes in vivo bone formation from hBMSCs. A- Xenograft tissue removed from the nude mice. B- Representative Micro CT scanning images of xenograft tissues. Scale bar, 500 μm. C-G- Bone volume or tissue volume (BV/TV) ( C ), bone surface/ tissue volume (BS/TV) ( D ), trabecular thickness (Tb.Th) ( E ), trabecular number (Tb.N) ( F ) and bone mineral density (BMD) ( G ) analyzed in xenograft tissues. H and I- HE staining ( H ) and Masson’s trichrome staining ( I ) of xenograft tissues. Scale bar, 50 μm. J-L - The expression levels of COL1A1 ( J ), RUNX2 ( K ), and OPN ( L ) in xenograft tissues evaluated by immunohistochemistry. Scale bar, 50 μm. * P < 0.05, ** P < 0.01, in comparison to NC/sh-NC. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13062-023-00425-2'>38017487</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-41467_2022_34287_fig2_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Downregulation of circBNC2 promotes epithelial cell G2/M arrest after injury. a Western blots showing DHX9 expression in lysates from HK2 cells exposed to hypoxia or AA for 24 h. b, c The primer sets designed in the pre-mRNA of BNC2 precursor ( b ) and the transcript abundance of amplicons a-d relative to input, detected by RNA immunoprecipitation with anti-DHX9 in lysates from HK2 cells treated with hypoxia for 24 h, followed by qRT-PCR assay ( c ). d qRT-PCR showing DHX9, circBNC2, lBNC2 and pre-mRNA of BNC2 (pmBNC2) expression in 24-h hypoxia-treated HK2 cells transfected with siRNAs targeting DHX9. e The top 10 significantly enriched Gene Ontology (GO) terms of the differentially expressed genes in mRNA sequencing of circBNC2-KO HK2 cells compared to wild-type (WT). f – h Cell cycle analysis by flow cytometry in HK2 cells, showing knockout of circBNC2 induced G2/M cell cycle arrest ( f , g ), especially G2 phase cell ( h ). i , j Immunofluorescence staining for p-H3 in circBNC2-KO HK2 cells showing increase in G2 phase positive cells ( i ) and the quantification data ( j ). See also Supplementary Fig. . k , l Western blots showing cyclin B1 and cyclin D1 expression in circBNC2-KO HK2 cells ( k ) and the ratio of cyclin B1/cyclin D1 ( l ). m , n Secretion of TGF-β1 ( m ) and CTGF ( n ) by circBNC2-KO HK2 cells was examined by ELISA. o qRT-PCR showing mRNA levels of αSMA , COL1A1 , FN , TGFB1 and CTGF expression in circBNC2-KO HK2 cells. p , q Western blots showing protein levels of αSMA, Col I, and FN in circBNC2-KO HK2 cells ( p ), and the quantification data ( q ). r The top 10 significantly enriched Gene Ontology (GO) terms of the differentially expressed genes in mRNA sequencing of circBNC2-KO L-02 cells compared to wild-type. s – u Cell cycle analysis by flow cytometry in L-02 cells showing knockout of circBNC2 induced G2/M cell cycle arrest ( s, t ), especially G2 phase cell cycle arrest ( u ). While overexpression of circBNC2 partially rescued the G2/M cell cycle arrest in circBNC2-KO cells. v Levels of TGF-β1 in supernatants of circBNC2-KO L-02 cells, examined by ELISA. w – y Western blots showing protein levels of αSMA and Col I in LX-2 cells incubated with conditional medium (C.M.) from circBNC2-KO L-02 cells or WT L-02 cells for 24 or 48 h. For c , d , g , h , j , l – o , q , t – v , x , y , n = 3 biologically independent cells. Data are expressed as means ± SD. Two-sided T -test was used for the comparison of two groups ( c , h , j , l , m , n , o , q , u , v , x , y ). One-way ANOVA with Bonferroni post hoc test was used for comparison among multiple groups ( d , g , t ). Source data are provided as a file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-34287-5'>36316334</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-1-col1a1-primary-antibodies-if-testing-4.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IF analysis of COL1A1 using anti-COL1A1 antibody (PA2140-1). <br> COL1A1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-COL1A1 Antibody (PA2140-1) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-i-antibody-pa2140-2-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-wb-testing-1_1_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: rat skin tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: mouse skin tissue lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I/COL1A1 antigen affinity purified polyclonal antibody (Catalog # PA2140-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I/COL1A1 at approximately 130 kDa, 220 kDa. The expected band size for Collagen I/COL1A1 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-ijbsv20p3412g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;FGF23 induces AVIC fibrosis and calcification. (A) Human AVICs from normal valves were treated with recombinant FGF23 in different concentrations for 72 hours. Representative immunoblots (left) and densitometric data (right) show that FGF23 upregulates the expression of inflammatory (ICAM-1 and VCAM-1), fibrogenic (collagen I and collagen IV) and osteogenic (RUNX2 and ALP) mediators in AVICs. (B) Representative images of Picrosirius Red (PSR) staining (upper) and Alizarin Red S staining (lower), along with corresponding spectrophotometric data, show that prolonged treatment with recombinant FGF23 (10 days or 14 days) induces collagen and calcium deposition in AVICs. Images were taken using a 10x objective. Scale bar = 100 µm. Quantitative data are expressed as mean ± SEM, n = 4 cell isolates from distinct donor valves in each group. * P < 0.05 versus untreated control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11234222/&orig_host=www.ncbi.nlm.nih.gov'>38993571</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-gr6_lrg.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Tpm1.6-silencing promotes collagen digestion upon TGF-β1 stimulation via induction of α-SMA puncta. Silenced-control and Tpm1.6-silenced NRK-49F cells were cultured on CG for 24 h, and then the CG was either released (A) or was treated with MMP inhibitors (GM600 or MMP9i, B-C) for 48 h with or without TGF-β1. Silenced-control and Tpm1.6-silenced cells were transfected with shSMA (D) or siCortactin (E) and cultured on unreleased CG with or without TGF-β1 for 48 h. The culture media was harvested for Western blotting analysis to detect collagen degradation. (A and F) Culture media collected from cells cultured on unreleased or released CG. Western blotting results showed Tpm1.6-silencing markedly increased collagen fragmentation (smaller than the size of monomer) upon TGF-β1. The release of CG to reduce traction force triggered the collagen degradation, and the quantification is shown in (F). Data are represented as mean ± SEM; n = 3 independent experiments were performed; two-way ANOVA; ∗p < 0.05, ∗∗∗∗p < 0.0001. (B, C, G, H) Western blotting results showed the effects of GM6001 (B) or MMP9 inhibitor (C) on TGF-β1-induced collagen degradation in Tpm1.6-silenced cells cultured on unreleased CG. Both GM6001 and MMP9 inhibitor completely suppressed TGF-β1-induced CG degradation in Tpm1.6-silenced cells, and the quantification is shown in (G) and (H), respectively. Data are represented as mean ± SEM; n = 3 independent experiments were performed; two-way ANOVA, ∗∗∗∗p < 0.0001. (D, E, I, J) Western blotting results showed the inhibition of α-SMA (non-canonical podosome) or cortactin (canonical podosome marker) markedly reduced TGF-β1-induced collagen degradation exerted by Tpm1.6-silenced cells, and the quantification is shown in (I) and (J), respectively. Data are represented as mean ± SEM; n = 3 independent experiments were performed; one-way ANOVA (I), two-way ANOVA, ∗∗p < 0.01, ∗∗∗∗p < 0.0001. (K) Cy3-conjugated gelatin was coated on a glass coverslip, and further silenced-control and Tpm1.6-silenced NRK-49F cells were cultured on it with or without TGF-β1 and MMP9 inhibitor for 48 h. The fluorescence loss indicates the gelatin degradation. TGF-β1-treated Tpm1.6-kd fibroblasts showed a matrix-degraded manner, and the treatment of MMP9i blocked the matrix degradation. Scale bars, 50 μm. (L) The observation of α-SMA dots (cyan) and the degraded region in the TGF-β1-treated Tpm1.6-kd group. Scale bars, 20 μm. (M–P) Silenced-control and Tpm1.6-silenced NRK-49F cells were transfected with liposome-embedded shRNA and siRNA against α-SMA (shSMA; M) and cortactin (siCortactin; N), and the cell lysates were analyzed by western blotting. Western blotting showed the protein levels of α-SMA or cortactin in cells silenced by shSMA or siCortactin, and quantification is shown in (O) and (P), respectively. Data are represented as mean ± SEM; n = 3 independent experiments were performed; Welch’s t test, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; data were compared to siNC.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/iscience/fulltext/S2589-0042(25)01578-0'>40917878</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-ihc-testing-2.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-ihc-testing-3_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of human lung adenocarcinoma cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-ihc-testing-4_1_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-ihc-testing-5_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-ihc-testing-6.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-ihc-testing-7_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-ihc-testing-8_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-if-testing-9_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-if-testing-10.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2140-2-col1a1-primary-antibodies-if-testing-11.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody (PA2140-2). <br> Collagen I/COL1A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I/COL1A1 Antibody (PA2140-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-ii-antibody-pa2141-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2141-1-WB-anti-collagen-ii-antibody.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;Anti-COL2A1 antibody&#44; PA2141&#44; Western blotting<br>All lanes: Anti COL2A1 (PA2141) at 0.5ug/ml<br>Lane 1: COLO320 Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: MCF-7Whole Cell Lysate at 40ug<br>Lane 4: A549 Whole Cell Lysate at 40ug<br>Lane 5: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 134 KD<br>Observed bind size: 170KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-ii-antibody-pa2141-1-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2141-1-1-IHC-anti-collagen-ii-antibody.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;Anti-Collagen II antibody&#44; PA2141-1&#44; IHC(F)<br>IHC(F): Rat Trachea Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-2_1.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;Anti-Collagen II antibody&#44; PA2141-1&#44; Western blotting<br>Lane 1: Rat Heart Tissue Lysate<br>Lane 1: Rat Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-jcmm-28-e70140-g006.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;Hoxc10 is retained in L/M‐BMSCs in vitro. (A) Schematic of Transwell co‐culture model of L‐BMSCs and M‐BMSCs. (B) Schematic of the limb bones and mandibles from different embryonic origins. The mandible is of neural crest origin (blue) and the limb bone is of mesodermal origin (orange) (C) qPCR verified the gene expression levels of Sox9 and Col2a1 before and after co‐culture of L‐BMSCs and M‐BMSCs. (D) The proliferation, osteogenic and chondrogenic genes of femoral homotopic grafting, femoral heterotopic grafting and mandibles homotopic grafting. (E) After 21 days of chondrogenic induction in the upper layer cells of Transwell model before and after co cultivation with L‐BMSCs and M‐BMSCs, blue stained proteoglycans were observed using Alcian blue. (F) Quantitative analysis of Alizarin blue staining before and after co culture of L‐BMSCs and M‐BMSCs. The data are presented as the mean ± SD ( n = 3). * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11493555/'>39434203</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-jcmm-28-e70140-g004.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;Hoxc10 is positively correlated with cartilage. n (C) q‐PCR validated the expression levels of Sox9 gene after overexpression and knockout of Hoxc10. (D) q‐PCR validated the expression levels of the Col2a1 gene after overexpression and knockout of Hoxc10. (E) q‐PCR validated the expression levels of Aggrecan gene after overexpression and knockout of Hoxc10. (F) The proteoglycan of BMSCs after overexpression and knockdown Hoxc10 was observed by Alcian blue staining 21 days after chondrogenic induction. (G) Quantitative analysis of Alcian blue staining. (H) Schematic diagram of co‐culture of L‐BMSCs and M‐BMSCs with and without Hoxc10 knockout. (I) Col2a1 gene expression in L‐BMSCs after Hoxc10 knockout and co‐culture with M‐BMSCs compared to control. (J) ChIP experiment of Sox9 and Hoxc10 protein binding. The data are presented as the mean ± SD ( n = 3).* p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11493555/'>39434203</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-13036_2018_119_fig5_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;Immunohistochemical staining of COL2A1 in vivo at indicated times postoperation. Scale bar: 200 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13036-018-0119-2'>30479659</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-13018_2024_5075_fig4_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;AF regulates ECM-related gene expression and Osteoclast-related Gene Expressions. A The mRNA expression of iNOS, COX-2, MMP13 and COL2A1 were analyzed using quantitative PCR. B The osteoclast-related gene expressions (c-fos, NFATc1, TRAP, DCSTAMP, calcitonin receptor, and V-ATPase d2) C Mice chondrocytes were pretreated with or without AF for 2 h followed by 0 or 10 ng/ml IL-1β for 48 h. D Quantification analysis of western blotting. E BMMs were pretreated with or without AF for 2 h followed by 0 or 50 ng/ml RANKL for 48 h. F Quantification analysis of western blotting. ## p < 0.01 versus the control group and * p < 0.05, ** p < 0.01 versus the IL‐1β treatment group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13018-024-05075-2'>39407273</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-13018_2024_5075_fig7_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;AF ameliorated OA progression in DMM-Induced OA mice model. A , B The effect of AF on DMM-stimulated cartilage degeneration was observed by HE Staining and Safranin O-Fast Green staining. E Quantitative analysis of OARSI scores. C , D Immunohistochemical (MMP13 and Col2a1) staining of mice chondrocytes in articular cartilage and quantitative analysis. (* p < 0.05; ** p < 0.01) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13018-024-05075-2'>39407273</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-fphar-15-1361561-g002.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;MSMP affected cartilage matrix metabolism in OA mice. (A) Immunohistochemical staining of Col2a1. (B) Statistical analysis of the positive expression for Col2a1. (C) Immunohistochemical staining of Mmp13. (D) Statistical analysis of the positive expression for Mmp13. Scale bar represents 100 μm (Date are mean ± SD, #### p < 0.0001, versus Sham group; * p < 0.05, ** p < 0.01, **** p < 0.0001, versus OA group).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1361561/full'>38974041</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-ejh-68-2-4033-g001.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;SPI can attenuate the ROS and damage of C28/I2 cells induced by TBHP. A ) Representative images of C28/I2 cells stained with phalloidin/DAPI after treatment with various doses of SPI for 24 h; scale bars: 200 μm. B ) Cell viability assessed using CCK8 assay after 24 h of SPI administration (n=3). C ) Percentage of DCFH-DA-positive C28/I2 cells detected by flow cytometry. D ) Quantitative analysis of the flow cytometry. E ) Western blot of Col II, MMP1, MMP3, MMP13, and β-actin protein levels in C28/I2 cells with different interventions. F-I ) Quantification analyses of Col II (F), MMP1 ( G ), MMP3 ( H ), and MMP13 ( I ) expression levels in C28/I2 cells with different interventions (n=3). * p <0.05, ** p <0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11148693/&orig_host=www.ncbi.nlm.nih.gov'>38779782</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-ejh-68-2-4033-g003.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;SPI attenuates the damage of C28/I2 cells induced by TBHP through the Nrf2 signaling pathway. A ) Western blot of Nrf2 and β-actin protein levels in C28/I2 cells with different interventions. B ) Quantification analysis of Nrf2 expression levels in C28/I2 cells with different interventions (n=3). C ) Western blot of Col II, Nrf2, MMP1, MMP13, and β-actin protein levels in C28/I2 cells with different interventions. D-G ) Quantification analyses of Col II ( D ), Nrf2 (E), MMP1 ( F ), and MMP13 ( G ) expression levels in C28/I2 cells with different interventions (n=3). * p <0.05, ** p <0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11148693/&orig_host=www.ncbi.nlm.nih.gov'>38779782</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-ejh-68-2-4033-g004.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;SPI can alleviate DMM-induced OA in mice. A ) Representative images of Safranin-O/Fast green staining of knee joints in mice with different interventions; scale bars: 200 μm. B ) The analysis of OARSI cartilage scoring on the knee joints of mice subjected to different interventions (n=6). C ) Representative images of Col II Immunohistochemistry staining in mice knee joints with different interventions. D ) Quantitative analysis of Col II expression levels in mice knee cartilage with different interventions (n=6); scale bars: 200 μm. ** p <0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11148693/&orig_host=www.ncbi.nlm.nih.gov'>38779782</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-41598_2023_43440_fig15_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;SiRNA TLR7 reversed collagen II and ADAMTS-5 degradation in IL-1β-stimulated chondrocytes. Cells were stimulated with IL-1β (10 ng/ml) for 48 h. The expression levels of collagen II and ADAMTS-5 were evaluated by Western blot ( a ) and quantification analysis ( b ). *** P < 0.001 versus the control group, ### P < 0.001 versus the IL-1β group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-023-43440-z'>37803031</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-13036_2018_119_fig2_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;UPR-related gene/protein expression in four groups. a Quantitative RT-PCR for ATF6 , ATF4 , and XBP1 of the four groups in the cultured constructs on 0, 7, 14, and 21 days. These data were normalized to GAPDH . b Western blot results for ATF4, ATF6, XBP1, and COL2A1 at different culture times. COL2A1 was not detectable at 0 day; β-actin was used as a loading control. c Normalized expression of ATF4, ATF6, XBP1, and COL2A1 in response to Western blot analysis. d Immunofluorescence staining of ATF4, ATF6, and XBP1 at 0 days. Scale bar: 50 μm. The values were means ± S.D.; * indicate P < 0.05, ** indicate P < 0.01, *** indicate P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13036-018-0119-2'>30479659</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-41413_2022_243_fig4_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;Fap degrades denatured or MMP13-cleaved Col II. a Fap degrades denatured Col II in a dose-dependent manner. Denatured Col II (boiled at 95 °C for 10 min) was incubated with different amounts of rFap at 37 °C for 24 h. Samples were separated by SDS‒PAGE and quantified by colloidal blue staining ( n = 3 independent experiments). b Fap degrades denatured Col II in a time-dependent manner. Denatured Col II was incubated with rFap at 37 °C for 0–24 h ( n = 3 independent experiments). c FAPi inhibits Fap-mediated degradation of denatured Col II. Different doses of FAPi were preincubated with rFap for 30 min before denatured Col II was added and further incubated at 37 °C for 24 h ( n = 3 independent experiments). d Immunoprecipitation of Fap. HEK293T cells were transfected with GFP control, Oln-Flag, Fap-HA, or Oln-Flag + Fap-HA. Two days after transfection, Fap was immunoprecipitated from total cell lysates with 10 μL anti-HA affinity gel. Five percent total cell lysates were loaded as an input control ( n = 3 independent experiments). e Oln inhibits the Fap-mediated degradation of denatured Col II. Col II was coincubated with immunoprecipitated samples (in 10 μL anti-HA affinity gel) at 37 °C for 24 h ( n = 3 independent experiments). f Fap degrades MMP13-cleaved Col II. Native Col II was preincubated with rFap or rMMP13 at 37 °C for 12 h. EDTA was then added to the reaction mixture with or without rFap and incubated at 37 °C for another 12 h ( n = 3 independent experiments). g Grayscale quantification of the 55, 40 and 30 kDa digestion bands in ( f ). h Experimental design. Primary chondrocytes were stimulated with vehicle control (PBS), 200 ng·mL −1 rFap, 200 ng·mL −1 rMMP13, or 200 ng·mL −1 rFap plus 200 ng·mL −1 rMMP13 for 24 h. i Western blot analysis of mouse Col2a1 protein levels in primary chondrocytes stimulated as in ( h ) ( n = 3 independent experiments). The statistical significance was assessed using one-way ANOVAs with Tukey’s multiple comparison tests. Data are presented as the mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41413-022-00243-8'>36588124</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-13036_2018_119_fig3_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;Chondrogenic-related gene/protein expression in four groups. a Quantitative RT-PCR for ACAN , SOX9 , COL2A1 , and COL1A1 of the four groups in the cultured constructs on 7, 14, and 21 days. These data were normalized to GAPDH . b Immunofluorescence staining of COL2A1 after 21 days of chondrogenic induction. Scale bar: 25 μm. c The semi-quantitative analysis of positive staining of COL2A1 in response to Fig. b. d MTT assay was used to analyze cell proliferation of the four groups: P0 BMSCs, P0 + 4-PBA, P3 BMSCs and P3 + TM. e The DNA and GAG contents were stained for the Hoechst 33258 and dimethylmethylene blue dye binding assays, respectively, after 7, 14 and 21 days of chondrogenic induction, and the absorbances were measured to quantify the contents of DNA and GAG. The left panel shows the DNA content and the right panel shows the ratio between GAG and DNA content. f GAG production was examined using Safranin-O staining in the four samples: P0 BMSCs, P0 with 4-PBA treatment, P3 BMSCs and P3 with TM treatment at 21 days. Scale bar: 100 μm. The values are means ± S.D.; * indicate P < 0.05, ** indicate P < 0.01, *** indicate P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13036-018-0119-2'>30479659</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-13287_2025_4422_fig7_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;DMOG enhances the therapeutic potential of senescent MSCs to mitigate metabolic dysfunction in OA chondrocytes. ( A ) Schematic diagram of the co-culture system. Human articular chondrocytes were treated with IL-1β to induce OA-like conditions and co-cultured with MSCs from various experimental groups using a Transwell system. ( B ) Representative immunofluorescence staining of ECM-related proteins, including Collagen II (Col2a1), Aggrecan (AGC), and MMP13, in OA chondrocytes co-cultured with MSCs from different experimental groups. Scale bar = 50 μm. ( C ) Quantitative analysis of immunofluorescence intensity for Col2a1, AGC, and MMP13. ( D ) Quantitative real-time PCR (qRT-PCR) analysis of mRNA levels for Col2a1, AGC, and MMP13 in OA chondrocytes co-cultured with MSCs. Data are expressed as mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-025-04422-2'>40457488</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1_13287_2025_4439_fig2_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;The chondrogenic differentiation and proliferation of cathepsin K (Ctsk) + CSPCs are activated in the MA mouse model. A Representative type II collagen (Col2) immunofluorescence staining images of condyles from tdTomato; Ctsk-Cre mice at 3 and 14 days old. Arrows indicate Ctsk and Col2 double-positive cells in the condylar cartilage. Scale bar: 50 μm. B The schematic diagram displays the workflow of the MA model establishment in tdTomato; Ctsk-Cre mice. C The lower incisors of tdTomato; Ctsk-Cre mice were trimmed by 1 mm every 3 days for 2 weeks to achieve MA. D Representative Col2 immunofluorescence staining images of condyles from tdTomato; Ctsk-Cre mice after 1 and 2 weeks of Sham or MA operation. Scale bar: 100 μm. E Representative EdU immunofluorescence staining images of condyles from tdTomato; Ctsk-Cre mice after 1 and 2 weeks of Sham or MA operation. Scale bar: 100 μm. F Quantitative analysis of the percentage of Ctsk, Col2, and Ctsk, EdU double-positive cells in the condylar cartilage from tdTomato; Ctsk-Cre mice in the Sham and MA groups, n = 5. Data are presented as mean ± SD. *** p < 0.001; ns, not significant <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-025-04439-7'>40598380</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1_gr1_lrg.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;SESN2 deficiency is correlated with the upregulation of lipogenic enzymes in OA cartilage (A) Safranin O-Fast Green (S.O.) and Alcian blue staining of intact (I) and damaged (D) articular cartilages from OA patients. (B) Immunohistochemical (IHC) staining of matrix metallopeptidase 13 (MMP13) and type II collagen gene α (COL2A1) in human OA cartilages. (C and D) IHC staining and quantitative analysis for SESN2 in intact (I) and damaged (D) human OA cartilages (n = 6). (E) Immunofluorescence (IF) staining for SESN2 expression in the cartilage of sham or destabilization of medial meniscus (DMM)-induced mice for 8 weeks (n = 6). (F) Indicated age mice S.O. staining and IF staining of knee joints from mice at indicated ages (8, 16, 28 weeks, 1 year) (n = 3). (G) Quantitative analysis for SESN2 expression in the cartilage of indicated treatment mice (n = 6) and indicated age mice (n = 3). (H and I) IHC staining (H) and quantitative analysis (I) for SESN2 in intact (I) and damaged (D) human OA cartilages (n = 6). (J and K) Relative mRNA expression (qPCR) (J) and correlation analysis quantification (K) of indicated proteins genes (SESN2 vs. MMP3, MMP13, FASN, SCD1) in paired intact (I) and damaged (D) (n = 8) human OA cartilages. Scale bars, (B, C, H, and F) 25 and 100 μm, (E) 50 μm, (A) 200 μm. Data are shown as mean ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. Statistical analysis was performed by unpaired t test (D, G [left], I, and J), one-way ANOVA (G, right), and Pearson correlation analysis (K).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/iscience/fulltext/S2589-0042(25)01358-6'>40822351</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1_gr5_lrg.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;SESN2 overexpression improves fatty acid metabolism disorders and ameliorates cartilage degeneration (A and B) IF (A) and corresponding quantitative analysis (B) of SESN2 in the articular cartilage of mice induced by sham or destabilization of medial meniscus (DMM) surgery with intraarticular injection of lv-nc and lv-Sesn2 (n = 6). (C–E) S.O. staining (C) of mice treated as in (A) (n = 6). Quantitation of Osteoarthritis Research Society International (OARSI) scores (D), chondrocyte numbers, and cartilage thickness (E) (n = 6). (F and G) IHC (F) and corresponding quantitative analysis (G) of COL2A1 and MMP13 in the articular cartilage of mice treated as in (A) (n = 6). (H and I) BODIPY493/503 staining, IF (H) of SREBP1, FASN, and SCD1, and corresponding quantitative analysis (I) in the articular cartilage of mice treated as in (A) (n = 6). (J and K) IF of PGC-1α and TUNEL staining (J) and corresponding quantitative analysis (K) in the articular cartilage of mice treated as in (A) (n = 6). Scale bars: (F, H, and J) 50 μm, (C) 50 and 100 μm. Data (n = 6) are shown as mean ± SD. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001. Statistical analysis was performed by one-way ANOVA (B, D, E, G, I, and K).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/iscience/fulltext/S2589-0042(25)01358-6'>40822351</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-research.0316.fig.003.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;Magnetothermal suppression of macrophagic inflammation and chondrocyte ferroptosis by MNPs-TRPV1. (A) Cell viability of RAW264.7 macrophages treated with different concentrations of MNPs-TRPV1 with (w/) or without (w/o) AMF stimulation. (B) The inductively coupled plasma mass spectrometry (ICP-MS) detection of iron content per RAW264.7 cell after incubated with 5 mg/ml MNPs or MNPs-TRPV1 for 3, 6, and 12 h. (C and D) Flow cytometry analysis (C) and quantitative analysis (D) of sulfo-cyanine3 (Cy3) conjugated MNPs or MNPs-TRPV1 incubated RAW264.7 cells for 12 h. The representative image presented in (C) showed the combination of MNPs-TRPV1 to the plasma membrane of RAW264.7 cell. (E) Western blot analysis of the expression levels of CaMKII and p-CaMKII in RAW264.7 cells 0, 5, 10, and 15 min after the treatment of MNPs-TRPV1 with AMF stimulation. (F) qPCR analysis of inflammatory genes, including Il-1β, Il-6, Tnf-α, Il-18, and Ptgs2, in RAW264.7 cells treated with or without 50 ng ml−1 LPS in the presence or absence of MNP-TRPV1 pretreatment under AMF exposure. (G) Western blot analysis (left panel) and corresponding quantitative analysis (right panel) of the expression of inflammatory proteins, iNos and Cox2, expressed in RAW264.7 cells induced as indicated. (H) Immunofluorescence staining (left panel) and quantitative analysis (right panel) of iNos and Cox2 in RAW264.7 cell induced as indicated. (I) Cell viability of mouse primary chondrocytes treated with various concentrations of MNPs-TRPV1 with (w/) or without (w/o) AMF stimulation. (J) The ICP-MS detection of iron content per chondrocyte after incubated with 5 mg/ml MNPs or MNPs-TRPV1 for 3, 6, and 12 h. (K and L) Flow cytometry analysis (C) and quantification (D) of Cy3-conjugated MNPs or MNPs-TRPV1 incubated chondrocytes for 12 h. The representative image presented in (K) showed the combination of MNPs-TRPV1 to the plasma membrane of chondrocyte. (M) The proteins expression levels of CaMKII and p-CaMKII in chondrocytes treated as indicated at the time point of 0, 5, 10, and 15 min. (N) qPCR analysis of gene expression levels of ferroptosis suppressors (Gpx4, Fth1, Slc7a11, and Cd44), ferroptosis drivers (Ptgs2, Ncoa4, Cdo1, Atf3, Pgd, and Tfrc), cartilage anabolic marker (Col2a1), and cartilage catabolic marker (Mmp13) in chondrocytes treated as indicated. (O and P) Representative images (left panel) and quantification (right panel) of ROS (O) and lipid ROS (P) levels in mouse primary chondrocytes treated with TBHP (T) or T + MNPs-TRPV1 under the stimulation of AMF. (Q) Western blot analysis of expression levels of Col II, Mmp13, Fth1, and Gpx4 in chondrocytes treated as indicated. w/o, without; w/, with. Scale bars, 15 μm (C and K), 20 μm (H) and 50 μm (O and P). One-way (D, F, G, H, and L) or 2-way (A, B, I, J, O, and P) ANOVA with Tukey’s post hoc test. Data are shown as mean ± SD. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://spj.science.org/doi/full/10.34133/research.0316'>38371274</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-1-s2.0-s2352304224001338-gr1.jpgAnti-Collagen II/COL2A1 Antibody Picoband&reg;Down-regulation of Notch1 signaling promoted BMP2-induced chondrogenic differentiation and inhibited BMP2-induced osteogenic differentiation of MSCs in vitro. (A) C3H10T1/2 cells were transfected with AdGFP, AdBMP2, AdDnNotch1, and AdBMP2+DnNotch1, respectively. On day 7, quantitative reverse transcription PCR was used to detect the expression of chondrogenic differentiation markers (Sox9 and Col2a1) and osteoblastic differentiation markers (Runx2, Col1a1, and OPN) of MSCs. (B) To detect the expression of sulfated glycosaminoglycan during C3H10T1/2 cell differentiation, alcian blue staining was performed on day 7 after cells were transfected with recombinant adenovirus. (C) Alkaline phosphatase (ALP) staining experiments were used to determine ALP activity on day 3 (a) and day 7 (b) respectively. (D) For matrix mineralization, alizarin red S staining was performed on day 14 (a); microscopic (b) observations showed that down-regulation of Notch1 signaling inhibited BMP2-induced calcium deposition. (E) Quantitative analysis of ALP activities and calcium deposition. The ALP activity was quantified at OD 405 nm and normalized by protein concentration per well (unit/mg protein) on day 3 (a) and day 7 (b). Alizarin red staining was quantified at OD 405 nm and normalized to total DNA per well (OD405 nm/μg DNA) (c). (F) Western blot analysis for the chondrogenic differentiation markers Sox9 and Col2a1 and the osteogenic markers Runx2, Col1a1, and OPN. Protein bands (a) and quantitative analysis (b). The relative expression of Sox9, Col2a1, Runx2, Col1a1, and OPN proteins were analyzed using GAPDH as control (b). One-way analysis of variance; ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05 versus the AdGFP group; ####p < 0.0001, ###p < 0.001, ##p < 0.01, and #p < 0.05 versus the indicated group; ns, p > 0.05. BMP2, bone morphogenetic protein 2; Col1a1, collagen type I alpha 1 chain; Col2a1, collagen type II alpha 1 chain; MSC, mesenchymal stem cell; Notch1, Notch receptor 1; OPN, osteopontin; Runx2, RUNX family transcription factor 2; Sox9; SRY-box transcription factor 9. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2352304224001338'>40083323</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-41467_2025_64361_fig3_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;Articular cartilage extracellular matrix disorders in Vgll4 CKO mice. A Schematic illustration of the construction of an animal model of articular chondrocyte-specific Vgll4 knockout in mice. CKO: Col2CreERT2; Vgll4 fl/fl . B Immunofluorescence staining for VGLL4 expression was performed on frozen sections of joints from 30-week-old CKO and littermate control mice. Red: VGLL4; blue: DAPI. Scale bar = 100 μm. C Workflow of the experiments used to evaluate the osteoarthritic phenotypes of the CKO mice after DMM surgery. D Representative micro-CT scans showing the volumes of the calcified meniscus and synovium (Marked in red). mice: n = 9, 8, 9, 8. E Quantification of calcified meniscus and synovial tissue volume in ( D ). Ordinary one-way ANOVA, means ± SEMs. mice: n = 9, 8, 9, 8. F SO&FG staining of female mouse knee joints. Scale bar = 500 μm (top), 200 μm (bottom). mice: n = 9, 8, 9, 8. The image below is an enlarged image of a portion of the above image. G Quantification of the indicated OARSI score in ( F ). Ordinary one-way ANOVA, means ± SEMs. mice: n = 9, 8, 9, 8. H Quantification of the relative cartilage thickness in ( F ). Ordinary one-way ANOVA, means ± SEMs. mice: n = 9, 8, 9, 8. I Quantification of the indicated relative SBP thickness in ( F ). SBP, subchondral bone plate. Ordinary one-way ANOVA, means ± SEMs. mice: n = 9, 8, 9, 8. J Immunofluorescence staining for COL2 expression was performed on paraffin-embedded joint sections. Scale bar = 200 μm. K Quantitative statistics of the proportion of COL2-positive cell areas (%) in ( J ) were performed by ImageJ software. Ordinary one-way ANOVA, means ± SEMs. n = 6 samples per group. L Immunofluorescence staining for COL1 expression was performed on paraffin-embedded joint sections. Scale bar = 200 μm. M Quantitative statistics of the proportion of COL1-positive cell areas (%) in ( L ) were performed by ImageJ software. Ordinary one-way ANOVA, means ± SEMs. n = 6 samples per group. N VGLL4 expression was reduced after cartilage injury, and VGLL4 deficiency resulting in hyaline cartilage degradation and transformation to fibrocartilage. Fig. c, : Created in BioRender. Suo, J. (2025) . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64361-7'>41125571</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-41467_2025_64361_fig4_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;TEAD4 mediates the interactions between VGLL4 and SMAD3. A KEGG analysis of downregulated genes in Vgll4 –/– mouse chondrocytes. B Schematic of the VGLL4 TDU domain at the full-length position of the VGLL4 protein. The TEAD4 protein binds to the TDU domain (top). Schematic of the VGLL4 ΔTDU build (bottom). C Co-immunoprecipitation experiments of SMAD3 (HA tag) and VGLL4/VGLL4 ΔTDU (Flag tag) in HEK-293T cells. IP: Flag. D Chondrocytes infected with Gfp (Control), Vgll4 or Vgll4 Δ TDU lentivirus were cultured in micromass and stained with alcian blue on the 7th day of induced chondrocyte differentiation (top). RT‒PCR was performed to determine the expression levels of Col2a1 (bottom). Boxes: 25th–75th percentiles; centre line: median; whiskers: 1.5×IQR. E GST pulldown analysis of purified GST-SMAD3 and VGLL4 TDU proteins. F Overall SMAD3-TEAD4-VGLL4 complex structural model predicted by AlphaFold2. SMAD3, TEAD4 and VGLL4 are shown in cyan, green and magenta, respectively. G Co-immunoprecipitation experiments of SMAD3 (HA tag) and TEAD1/2/3/4 (Flag tag) in HEK-293T cells. IP: Flag. H Co-immunoprecipitation experiments of TEAD4 (HA tag), SMAD3 (Flag tag) and VGLL4 (Myc tag) in HEK-293T cells. IP: Flag. I Co-immunoprecipitation experiments of SMAD3 and TEADs in the chondrocytes of the Vgll4 fl/fl mice treated with GFP or Cre lentivirus. IP: Pan-TEAD. J RT‒PCR was performed to determine the gene expression of Col2a1 and Acan in chondrocytes after infection with the Control, Smad3 , Vgll4 or Smad3 + Vgll4 lentivirus in chondrocytes. Ordinary one-way ANOVA was used, and the data are presented as the means ± SEMs. n = 4 per group. Boxes: 25th–75th percentiles; centre line: median; whiskers: 1.5×IQR. K Model diagram of the function of the VGLL4-TEAD4-SMAD3 complex. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64361-7'>41125571</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-41467_2025_64361_fig5_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;SMAD3 depends on binding to TEADs to alleviate OA. A Interface between SMAD3 and TEAD4. The residues involved in hydrophobic contact and hydrogen bonds (orange dashed lines) are labeled. B Co-immunoprecipitation experiments of TEAD4 and TEAD4 mut4 (HA tag), VGLL4 (Myc tag), and SMAD3 (Flag tag) in HEK-293T cells. IP: Flag. C Co-immunoprecipitation experiments of TEAD4 (Myc tag), SMAD3 and Smad3 K81A/F260A (Flag tag) in HEK-293T cells. IP: Flag. D Co-immunoprecipitation experiments of TEAD4 (HA tag), VGLL4 (Myc tag), SMAD3 and Smad3 K81A/F260A (Flag tag) in HEK-293T cells. IP: Flag. E RT‒PCR analysis of Col2a1 gene expression in chondrocytes after infection with the Control, Smad3 , Vgll4 , Smad3 + Vgll4 or Smad3 K81A/F260A + Vgll4 lentivirus. Ordinary one-way ANOVA was used, and the data are presented as the means ± SEMs. n = 3 per group. F Co-immunoprecipitation experiments of SMAD3 (HA tag) and TEAD4/TEAD4 Y429H (Flag tag) in HEK-293T cells. IP: Flag. G Experimental procedure for assessing the effects of articular injection of Smad3 and Smad3 KF2A AAV on the osteoarthritic phenotype in wild-type mice after sham or DMM surgery. H Representative micro-CT scans showing the volumes of the calcified meniscus and synovium (Marked in red). n = 12 samples per group. I Quantification of calcified meniscus and synovial tissue volume in ( H ). Ordinary one-way ANOVA was used, and the data are presented as the means ± SEMs. n = 12 samples per group. J SO&FG staining of sections of the mouse knee joint. Scale bar = 500 μm (top), 200 μm (bottom). n = 10 samples per group. The image below is an enlarged image of a portion of the above image. K Quantification of the indicated OARSI score in ( J ). Ordinary one-way ANOVA, means ± SEMs. n = 10 samples per group. L Quantification of the relative cartilage thickness in ( J ). Ordinary one-way ANOVA, means ± SEMs. n = 10 samples per group. M Quantification of the indicated relative SBP thickness in ( J ). SBP, subchondral bone plate. Ordinary one-way ANOVA, means ± SEMs. n = 10 samples per group. Fig. : Created in BioRender. Suo, J. (2025) . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64361-7'>41125571</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-41467_2025_64361_fig6_html.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;VGLL4 increases the binding of TEAD4 to SMAD3 and promotes extracellular matrix homeostasis. A Co-immunoprecipitation experiments of TEAD4 (Flag tag), SMAD3 (HA tag), VGLL4 (Myc tag) and VGLL4 ΔTDU (Myc tag) in HEK-293T cells. IP: Flag. B GST pulldown analysis of purified GST-SMAD3, VGLL4 TDU and Flag TEAD4 proteins. C The TEAD4 binding pocket of VGLL4. The residues involved in the π-π-π-π and van der Waals interactions are shown as stick models. D Co-immunoprecipitation experiments of VGLL4 and VGLL4 HF4A (Myc tag), SMAD3 (Flag tag) and TEAD4 (HA tag) in HEK-293T cells. IP: Flag. E Experimental procedure to assess the effect of articular injection of Vgll4 and Vgll4 HF4A AAV on the osteoarthritic phenotype in wild-type mice after DMM surgery. F Representative micro-CT scans showing the volumes of the calcified meniscus and synovium (Marked in red). mice: n = 10, 9, 10, 10. G Quantification of calcified meniscus and synovial tissue volume in ( F ). Ordinary one-way ANOVA, means ± SEMs. mice: n = 10, 9, 10, 10. H SO&FG staining of sections of the mouse knee joint. Scale bar = 500 μm (top), 200 μm (bottom). mice: n = 10, 9, 10, 10. The image below is an enlarged image of a portion of the above image. I Quantification of the indicated OARSI score in ( H ). Ordinary one-way ANOVA, means ± SEMs. mice: n = 10, 9, 10, 10. J Quantification of the relative cartilage thickness in ( H ). Ordinary one-way ANOVA, means ± SEMs. mice: n = 10, 9, 10, 10. K Quantification of the indicated relative SBP thickness in ( H ). SBP, subchondral bone plate. Ordinary one-way ANOVA, means ± SEMs. mice: n = 10, 9, 10, 10. L Immunofluorescence staining for COL2 and COL1 expression was performed on paraffin-embedded joint sections. Scale bar = 200 μm. n = 6 Samples per group. M Quantitative statistics of the proportion of COL2 or COL1-positive cell areas (%) in ( L ) were performed by ImageJ software. Ordinary one-way ANOVA, means ± SEMs. n = 6 Samples per group. N Schematic representation of VGLL4 function in cartilage. Fig. : Created in BioRender. Suo, J. (2025) . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-64361-7'>41125571</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2141-1-col2a1-primary-antibodies-if-testing-1.pngAnti-Collagen II/COL2A1 Antibody Picoband&reg;IF analysis of COL2A1 using anti-COL2A1 antibody (PA2141-1). <br> COL2A1 was detected in a paraffin-embedded section of Mouse cartilage tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-COL2A1 Antibody (PA2141-1) overnight at 4°C. Donkey anti-rabbit Alexa Fluor 555was used as secondary antibody at 1:500 dilution and incubated for 1 hour at RT. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e2f3-antibody-pa2142-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2142-1-WB-anti-e2f3-antibody.jpgAnti-Transcription factor E2F3 E2F3 Antibody Picoband&reg;Anti-E2F3 antibody&#44; PA2142&#44; Western blotting<br>All lanes: Anti E2F3 (PA2142) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 49KD<br>Observed bind size: 49KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hif3-antibody-pa2145-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2145-1-WB-anti-hif3-antibody.jpgAnti-HIF3/HIF3A Antibody Picoband&reg;Anti-HIF3 antibody&#44; PA2145&#44; Western blotting<br>WB: Rat Brain Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp12-antibody-pa2146-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2146-1-WB-anti-mmp-12-antibody.jpgAnti-Macrophage metalloelastase MMP12 Antibody Picoband&reg;Anti-MMP12 antibody&#44; PA2146&#44; Western blotting<br>Lane 1: SMMC Cell Lysate<br>Lane 2: HEPA Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: K562 Cell Lysate<br>Lane 5: MCF-7 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp14-antibody-pa2147-boster.html2026-03-24T05:04:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2147-1-IHC-anti-mmp-14-antibody.jpgAnti-Matrix metalloproteinase-14 MMP14 Antibody Picoband&reg;Anti-MMP14 antibody&#44; PA2147&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2147-2-IHC-anti-mmp-14-antibody.jpgAnti-Matrix metalloproteinase-14 MMP14 Antibody Picoband&reg;Anti-MMP14 antibody&#44; PA2147&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2147-3-IHC-anti-mmp-14-antibody.jpgAnti-Matrix metalloproteinase-14 MMP14 Antibody Picoband&reg;Anti-MMP14 antibody&#44; PA2147&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2147-4-WB-anti-mmp-14-antibody.jpgAnti-Matrix metalloproteinase-14 MMP14 Antibody Picoband&reg;Anti-MMP14 antibody&#44; PA2147&#44; Western blotting<br>All lanes: Anti MMP14 (PA2147) at 0.5ug/ml<br>Lane 1: Rat Spleen Tissue Lysate at 50ug<br>Lane 2: Human Placenta Tissue Lysate at 50ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Lane 4: A549 Whole Cell Lysate at 40ug<br>Predicted bind size: 66KD<br>Observed bind size: 150KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ndrg2-antibody-pa2148-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2148-ndrg2-primary-antibodies-wb-testing-1.jpgAnti-Protein NDRG2 NDRG2 Antibody Picoband&reg;Western blot analysis of NDRG2 using anti-NDRG2 antibody (PA2148). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat heart tissue lysates,<br> Lane 4: mouse brain tissue lysates,<br> Lane 5: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG2 antigen affinity purified polyclonal antibody (PA2148) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDRG2 at approximately 41 kDa. The expected band size for NDRG2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2148-ndrg2-primary-antibodies-ihc-testing-1.jpgAnti-Protein NDRG2 NDRG2 Antibody Picoband&reg;IHC analysis of NDRG2 using anti-NDRG2 antibody (PA2148). <br>NDRG2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NDRG2 Antibody (PA2148) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trka-antibody-pa2149-1-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2149-1-1-WB-anti-trka-antibody.jpgAnti-TrkA/NTRK1 Antibody Picoband&reg;Anti-TrkA antibody&#44; PA2149-1&#44; Western blotting<br>Lane 1: COLO320 Cell Lysate<br>Lane 2: HT1080 Cell Lysate<br>Lane 3: U87 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trka-antibody-pa2149-2-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2149-2-1-WB-anti-trka-antibody.jpgAnti-TrkA/NTRK1 Antibody Picoband&reg;Anti-TrkA antibody&#44; PA2149-2&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclophilin-b-antibody-pa2151-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1959-ppib-primary-antibodies-wb-testing-1.jpgAnti-Cyclophilin B/PPIB Antibody Picoband&reg; Western blot analysis of Cyclophilin B/PPIB using anti-Cyclophilin B/PPIB antibody (PA2151). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human CACO-2 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat lung tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin B/PPIB antigen affinity purified polyclonal antibody (Catalog # PA2151) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin B/PPIB at approximately 18 kDa. The expected band size for Cyclophilin B/PPIB is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2151-2-IHC-anti-cyclophilin-b-antibody.jpgAnti-Cyclophilin B/PPIB Antibody Picoband&reg; IHC analysis of Cyclophilin B/PPIB using anti-Cyclophilin B/PPIB antibody (PA2151).<br> Cyclophilin B/PPIB was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cyclophilin B/PPIB Antibody (PA2151) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2151-3.jpgAnti-Cyclophilin B/PPIB Antibody Picoband&reg; IHC analysis of Cyclophilin B/PPIB using anti-Cyclophilin B/PPIB antibody (PA2151).<br> Cyclophilin B/PPIB was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cyclophilin B/PPIB Antibody (PA2151) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tgm2-antibody-pa2153-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2153-1-WB-anti-tgm2-transglutaminase-2-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg;Anti-TGM2 antibody&#44; PA2153&#44; Western blotting<br>Lane 1: JURKAT Cell Lysate<br>Lane 2: HELA Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2153-2-IHC-anti-tgm2-transglutaminase-2-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg;Anti-TGM2 antibody&#44; PA2153&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-traf5-antibody-pa2154-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2154-1-WB-anti-traf5-antibody.jpgAnti-TRAF5 Antibody Picoband&reg;Anti-TRAF5 antibody&#44; PA2154&#44; Western blotting<br>WB: k562 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beta-arrestin-1-antibody-pa2155-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2155-1-IHC-anti-beta-arrestin-1-antibody.jpgAnti-beta Arrestin 1/ARRB1 Antibody Picoband&reg;Anti-beta Arrestin 1 antibody&#44; PA2155&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2155-2-WB-anti-beta-arrestin-1-antibody.jpgAnti-beta Arrestin 1/ARRB1 Antibody Picoband&reg;Anti-beta Arrestin 1 antibody&#44; PA2155&#44; Western blotting<br>Lane 1: Rat Lung Tissue Lysate<br>Lane 2: Rat Skeletal Muscle Tissue Lysate<br>Lane 3: SW620 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e2f4-antibody-pa2156-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2156-1-WB-anti-e2f4-antibody.jpgAnti-Transcription factor E2F4 E2F4 Antibody Picoband&reg;Anti-E2F4 antibody&#44; PA2156&#44; Western blotting<br>All lanes: Anti E2F4 (PA2156) at 0.5ug/ml<br>Lane 1: Rat Lung Tissue Lysate at 50ug<br>Lane 2: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 44KD<br>Observed bind size: 44KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif2s2-antibody-pa2157-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2157-mag-primary-antibodies-wb-testing-1.jpgAnti-EIF2S2 Antibody Picoband&reg; Western blot analysis of EIF2S2 using anti-EIF2S2 antibody (PA2157). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S2 antigen affinity purified polyclonal antibody (Catalog # PA2157) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S2 at approximately 38 kDa. The expected band size for EIF2S2 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fabp6-antibody-pa2158-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2158-1-IHC-anti-fabp6-ilbp-antibody.jpgAnti-Gastrotropin FABP6 Antibody Picoband&reg;Anti-FABP6 antibody&#44; PA2158&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2158-2-WB-anti-fabp6-ilbp-antibody.jpgAnti-Gastrotropin FABP6 Antibody Picoband&reg;Anti-FABP6 antibody&#44; PA2158&#44; Western blotting<br>All lanes: Anti FABP6 (PA2158) at 0.5ug/ml<br>Lane 1: COLO320 Whole Cell Lysate at 40ug<br>Lane 2: SW620 Whole Cell Lysate at 40ug<br>Predicted bind size: 14KD<br>Observed bind size: 14KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-laminin-2-alpha-antibody-pa2160-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2160-1-WB-anti-laminin-2-alpha-antibody.jpgAnti-Laminin 2 alpha/LAMA2 Antibody Picoband&reg;Anti-Laminin 2 alpha antibody&#44; PA2160&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: A549 Cell Lysate<br>Lane 3: PANC Cell Lysate<br><br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-profilin-2-antibody-pa2162-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2162-pfn2-primary-antibodies-wb-testing-1.jpgAnti-Profilin 2/PFN2 Antibody Picoband&reg;Western blot analysis of PFN2 using anti-PFN2 antibody (PA2162). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human SH-SY5Y whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat skeletal muscle tissue lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse skeletal muscle tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFN2 antigen affinity purified polyclonal antibody (PA2162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PFN2 at approximately 15 kDa. The expected band size for PFN2 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2162-pfn2-primary-antibodies-ihc-testing-1.jpgAnti-Profilin 2/PFN2 Antibody Picoband&reg;IHC analysis of PFN2 using anti-PFN2 antibody (PA2162). <br>PFN2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PFN2 Antibody (PA2162) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2162-pfn2-primary-antibodies-if-testing-1.jpgAnti-Profilin 2/PFN2 Antibody Picoband&reg;IF analysis of PFN2 using anti-PFN2 antibody (PA2162). <br> PFN2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PFN2 Antibody (PA2162) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rgs14-antibody-pa2163-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2163-1-WB-anti-rgs14-antibody.jpgAnti-RGS14 Antibody Picoband&reg;Anti-RGS14 antibody&#44; PA2163&#44; Western blotting<br>Lane 1: Rat Thymus Tissue Lysate<br>Lane 2: Rat Spleen Tissue Lysate<br>Lane 3: RAJI Cell Lysate<br>Lane 4: CEM Cell Lysate<br>Lane 5: JURKAT Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eaat3-antibody-pa2164-1-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2164-1-1-WB-anti-eaat3-antibody.jpgAnti-EAAT3/SLC1A1 Antibody Picoband&reg;Anti-EAAT3 antibody&#44; PA2164-1&#44; Western blotting<br>WB: Human Placenta Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eaat3-antibody-pa2164-2-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2164-2-1-WB-anti-eaat3-antibody.jpgAnti-EAAT3/SLC1A1 Antibody Picoband&reg;Anti-EAAT3 antibody&#44; PA2164-2&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Rat Heart Tissue Lysate<br>Lane 3: HEPA Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glucose-transporter-8-antibody-pa2165-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2165-1-WB-anti-glucose-transporter-8-antibody.jpgAnti-Glucose Transporter 8/SLC2A8 Antibody Picoband&reg;Anti-Glucose Transporter 8 antibody&#44; PA2165&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: Human Placenta Tissue Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2165-2-IHC-anti-glucose-transporter-8-antibody.jpgAnti-Glucose Transporter 8/SLC2A8 Antibody Picoband&reg;Anti-Glucose Transporter 8 antibody&#44; PA2165&#44; IHC(P)<br>IHC(P): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2165-3-IHC-anti-glucose-transporter-8-antibody.jpgAnti-Glucose Transporter 8/SLC2A8 Antibody Picoband&reg;Anti-Glucose Transporter 8 antibody&#44; PA2165&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glut12-antibody-pa2167-boster.html2026-03-24T05:04:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2167-1-WB-anti-glut12-antibody.jpgAnti-GLUT12/SLC2A12 Antibody Picoband&reg;Anti-GLUT12 antibody&#44; PA2167&#44; Western blotting<br>Lane 1: PC-12 Cell Lysate<br>Lane 2: A549 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc12a1-antibody-pa2168-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2168-slc12a1-primary-antibodies-wb-testing-1.jpgAnti-SLC12A1 Antibody Picoband&reg;Western blot analysis of SLC12A1 using anti-SLC12A1 antibody (PA2168). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates,<br> Lane 2: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A1 antigen affinity purified polyclonal antibody (PA2168) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC12A1 at approximately 160-290 kDa. The expected band size for SLC12A1 is at 121 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2168-slc12a1-primary-antibodies-ihc-testing-1.jpgAnti-SLC12A1 Antibody Picoband&reg;IHC analysis of SLC12A1 using anti-SLC12A1 antibody (PA2168). <br>SLC12A1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A1 Antibody (PA2168) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2168-slc12a1-primary-antibodies-ihc-testing-2.jpgAnti-SLC12A1 Antibody Picoband&reg;IHC analysis of SLC12A1 using anti-SLC12A1 antibody (PA2168). <br>SLC12A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A1 Antibody (PA2168) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2168-slc12a1-primary-antibodies-ihc-testing-3.jpgAnti-SLC12A1 Antibody Picoband&reg;IHC analysis of SLC12A1 using anti-SLC12A1 antibody (PA2168). <br>SLC12A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A1 Antibody (PA2168) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nkcc1-antibody-pa2169-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2169-slc12a2-primary-antibodies-wb-testing-1.jpgAnti-NKCC1/SLC12A2 Antibody Picoband&reg; Western blot analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A2 antigen affinity purified polyclonal antibody (Catalog # PA2169) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A2 at approximately 200 kDa. The expected band size for SLC12A2 is at 131 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2169-slc12a2-primary-antibodies-ihc-testing-2.jpgAnti-NKCC1/SLC12A2 Antibody Picoband&reg; IHC analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169). <br> SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2169-slc12a2-primary-antibodies-ihc-testing-3.jpgAnti-NKCC1/SLC12A2 Antibody Picoband&reg; IHC analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169). <br> SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2169-slc12a2-primary-antibodies-ihc-testing-4.jpgAnti-NKCC1/SLC12A2 Antibody Picoband&reg; IHC analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169). <br> SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2169-slc12a2-primary-antibodies-if-testing-5.jpgAnti-NKCC1/SLC12A2 Antibody Picoband&reg; IF analysis of SLC12A2 using anti-SLC12A2 antibody (PA2169). <br> SLC12A2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC12A2 Antibody (PA2169) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc12a6-antibody-pa2171-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2171-slc12a6-primary-antibodies-wb-testing-1.jpgAnti-SLC12A6 Antibody Picoband&reg; Western blot analysis of SLC12A6 using anti-SLC12A6 antibody (PA2171). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat C6 whole cell lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A6 antigen affinity purified polyclonal antibody (Catalog # PA2171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A6 at approximately 128 kDa. The expected band size for SLC12A6 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2171-slc12a6-primary-antibodies-ihc-testing-2.jpgAnti-SLC12A6 Antibody Picoband&reg; IHC analysis of SLC12A6 using anti-SLC12A6 antibody (PA2171). <br> SLC12A6 was detected in a paraffin-embedded section of human colorectal adenocarcinomar tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A6 Antibody (PA2171) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc22a1-antibody-pa2172-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2172-1-WB-anti-slc22a1-antibody.jpgAnti-SLC22A1 Antibody Picoband&reg;Anti-SLC22A1 antibody&#44; PA2172&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: A549 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-solute-carrier-family-22-member-5-antibody-pa2174-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2174-1-WB-anti-solute-carrier-family-22-member-5-antibody.jpgAnti-Solute carrier family 22 member 5/SLC22A5 Antibody Picoband&reg;Anti-Solute carrier family 22 member 5 antibody&#44; PA2174&#44; Western blotting<br>Lane 1: Rat Kidney Tissue Lysate<br>Lane 2: Rat Skeletal Muscles Tissue Lysate<br>Lane 3: Mouse Liver Tissue Lysate<br>Lane 4: HEPA Cell Lysate<br>Lane 5: NIH3T3 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccr3-antibody-pa2176-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2176-1-WB-anti-ccr3-antibody.jpgAnti-CCR3 Antibody Picoband&reg;Anti-CCR3 antibody&#44; PA2176&#44; Western blotting<br>Lane 1: K562 Cell Lysate<br>Lane 2: RAJI Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2176-2-IHC-anti-ccr3-antibody.jpgAnti-CCR3 Antibody Picoband&reg;Anti-CCR3 antibody&#44; PA2176&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egr1-antibody-pa2177-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Egr1 Antibody Picoband&reg;Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egr2-antibody-pa2178-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2178-egr2-primary-antibodies-wb-testing-1.jpgAnti-EGR2 Antibody Picoband&reg;Western blot analysis of EGR2 using anti-EGR2 antibody (PA2178). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGR2 antigen affinity purified polyclonal antibody (PA2178) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EGR2 at approximately 55 kDa. The expected band size for EGR2 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2178-egr2-primary-antibodies-if-testing-1.jpgAnti-EGR2 Antibody Picoband&reg;IF analysis of EGR2 using anti-EGR2 antibody (PA2178) and anti-Tubulin Alpha antibody (M03989-3).<br> EGR2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-EGR2 Antibody (PA2178) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fli1-antibody-pa2179-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2179-1-IHC-anti-fli1-antibody.jpgAnti-FLI1 Antibody Picoband&reg;Anti-FLI1 antibody&#44; PA2179&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2179-2-WB-anti-fli1-antibody.jpgAnti-FLI1 Antibody Picoband&reg;Anti-FLI1 antibody&#44; PA2179&#44; Western blotting<br>Lane 1: JURKAT Cell Lysate<br>Lane 2: RAJI Cell Lysate<br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2179-3-IHC-anti-fli1-antibody.jpgAnti-FLI1 Antibody Picoband&reg;Anti-FLI1 antibody&#44; PA2179&#44; IHC(P)<br>IHC(P): Rat Spleen Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdac5-antibody-pa2180-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2180-1-WB-anti-hdac5-antibody.jpgAnti-Histone deacetylase 5 HDAC5 Antibody Picoband&reg;Anti-HDAC5 antibody&#44; PA2180&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: COLO320 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hyal1-antibody-pa2181-1-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2181-1-hyal1-primary-antibodies-wb-testing-1.jpgAnti-Hyaluronidase-1 HYAL1 Antibody Picoband&reg; Western blot analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: monkey COS-7 whole cell lysates, <br> Lane 4: rat liver tissue lysates, <br> Lane 5: mouse liver tissue lysates, <br> Lane 6: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HYAL1 antigen affinity purified polyclonal antibody (Catalog # PA2181-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HYAL1 at approximately 60 kDa. The expected band size for HYAL1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2181-1-hyal1-primary-antibodies-ihc-testing-2.jpgAnti-Hyaluronidase-1 HYAL1 Antibody Picoband&reg; IHC analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1). <br> HYAL1 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HYAL1 Antibody (PA2181-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2181-1-hyal1-primary-antibodies-icc-testing-3.jpgAnti-Hyaluronidase-1 HYAL1 Antibody Picoband&reg; ICC analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1). <br> HYAL1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-HYAL1 Antibody (PA2181-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2181-1-hyal1-primary-antibodies-ihc-f-testing-4.jpgAnti-Hyaluronidase-1 HYAL1 Antibody Picoband&reg; IHC analysis of HYAL1 using anti-HYAL1 antibody (PA2181-1). <br> HYAL1 was detected in a frozen section of Human Placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HYAL1 Antibody (PA2181-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hyal1-antibody-pa2181-2-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2181-2-1-WB-anti-hyal1-antibody.jpgAnti-Hyaluronidase-1 HYAL1 Antibody Picoband&reg;Anti-HYAL1 antibody&#44; PA2181-2&#44; Western blotting<br>Lane 1: NRK Cell Lysate<br>Lane 2: Mouse Liver Tissue Lysate<br>Lane 3: Mouse Kidney Tissue Lysate<br>Lane 4: SP2/0 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mannose-6-phosphate-receptor-cation-independent-antibody-pa2182-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2182-igf2r-primary-antibodies-wb-testing-1.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband&reg; Western blot analysis of IGF2R using anti-IGF2R antibody (PA2182). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human U87 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGF2R antigen affinity purified polyclonal antibody (Catalog # PA2182) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGF2R at approximately 274 kDa. The expected band size for IGF2R is at 274 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2182-igf2r-primary-antibodies-fcm-testing-2.jpgAnti-Mannose 6 Phosphate Receptor (Cation independent)/IGF2R Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-IGF2R antibody (PA2182). <br> Overlay histogram showing HepG2 cells stained with PA2182 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IGF2R Antibody (PA2182, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irak-antibody-pa2183-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2183-1-WB-anti-irak-antibody.jpgAnti-IRAK/IRAK1 Antibody Picoband&reg;Anti-IRAK antibody&#44; PA2183&#44; Western blotting<br>Lane 1: Rat Liver Tissue Lysate<br>Lane 2: Human Placenta Tissue Lysate<br>Lane 3: MCF-7 Cell Lysate<br>Lane 4: PANC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eaat1-antibody-pa2185-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2185-slc1a3-primary-antibodies-wb-testing-1.jpgAnti-EAAT1/SLC1A3 Antibody Picoband&reg;Western blot analysis of GLAST/SLC1A3 using anti-GLAST/SLC1A3 antibody (PA2185). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLAST/SLC1A3 antigen affinity purified polyclonal antibody (PA2185) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLAST/SLC1A3 at approximately 60 kDa. The expected band size for GLAST/SLC1A3 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2185-slc1a3-primary-antibodies-ihc-testing-1.jpgAnti-EAAT1/SLC1A3 Antibody Picoband&reg;IHC analysis of GLAST/SLC1A3 using anti-GLAST/SLC1A3 antibody (PA2185). <br>GLAST/SLC1A3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLAST/SLC1A3 Antibody (PA2185) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2185-slc1a3-primary-antibodies-ihc-testing-2.jpgAnti-EAAT1/SLC1A3 Antibody Picoband&reg;IHC analysis of GLAST/SLC1A3 using anti-GLAST/SLC1A3 antibody (PA2185). <br>GLAST/SLC1A3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLAST/SLC1A3 Antibody (PA2185) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2185-slc1a3-primary-antibodies-ihc-testing-3.jpgAnti-EAAT1/SLC1A3 Antibody Picoband&reg;IHC analysis of GLAST/SLC1A3 using anti-GLAST/SLC1A3 antibody (PA2185). <br>GLAST/SLC1A3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLAST/SLC1A3 Antibody (PA2185) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc1a4-antibody-pa2186-boster.html2026-03-24T05:04:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2186-1-WB-anti-slc1a4-asct1-antibody.jpgAnti-SLC1A4 Antibody Picoband&reg;Anti-SLC1A4 antibody&#44; PA2186&#44; Western blotting<br>Lane 1: U87 Cell Lysate<br>Lane 2: Rat Brain Tissue Lysate<br>Lane 3: Mouse Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2186-2-IHC-anti-slc1a4-asct1-antibody.jpgAnti-SLC1A4 Antibody Picoband&reg;Anti-SLC1A4 antibody&#44; PA2186&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc16a4-antibody-pa2189-boster.html2026-03-25T05:21:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2189-slc16a4-primary-antibodies-wb-testing-1.jpgAnti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband&reg;Western blot analysis of SLC16A4 using anti-SLC16A4 antibody (PA2189). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: rat NRK whole cell lysates,<br> Lane 4: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC16A4 antigen affinity purified polyclonal antibody (PA2189) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC16A4 at approximately 54 kDa. The expected band size for SLC16A4 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2189-slc16a4-primary-antibodies-ihc-testing-1.jpgAnti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband&reg;IHC analysis of SLC16A4 using anti-SLC16A4 antibody (PA2189). <br>SLC16A4 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC16A4 Antibody (PA2189) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2189-slc16a4-primary-antibodies-if-testing-1.jpgAnti-Monocarboxylate transporter 5 SLC16A4 Antibody Picoband&reg;IF analysis of SLC16A4 using anti-SLC16A4 antibody (PA2189). <br> SLC16A4 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SLC16A4 Antibody (PA2189) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tia1-antibody-pa2194-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2194-1-WB-anti-tia1-antibody.jpgAnti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody Picoband&reg;Anti-TIA1 antibody&#44; PA2194&#44; Western blotting<br>Lane 1: JURKAT Cell Lysate<br>Lane 2: RAJI Cell Lysate<br>Lane 3: CEM Cell Lysate<br>Lane 4: HT1080 Cell Lysate<br>Lane 5: K562 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-leupaxin-antibody-pa2195-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2195-lpxn-primary-antibodies-wb-testing-1.jpgAnti-Leupaxin/LPXN Antibody Picoband&reg; Western blot analysis of LPXN using anti-LPXN antibody (PA2195). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human PC-3 whole cell lysates,<br> Lane 3: human U87 whole cell lysates,<br> Lane 4: human MDA-MB-453 whole cell lysates,<br> Lane 5: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LPXN antigen affinity purified polyclonal antibody (Catalog # PA2195) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LPXN at approximately 43 kDa. The expected band size for LPXN is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2195-lpxn-primary-antibodies-fcm-testing-2.jpgAnti-Leupaxin/LPXN Antibody Picoband&reg; Flow Cytometry analysis of Raji cells using anti-LPXN antibody (PA2195). <br> Overlay histogram showing Raji cells stained with PA2195 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LPXN Antibody (PA2195, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angiotensin-converting-enzyme-1-antibody-pa2196-1-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2196-1-2-IHC-anti-ace-cd143-antibody.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg;Anti-ACE antibody&#44; PA2196-1&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2196-1-1-IHC-anti-ace-cd143-antibody.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg;Anti-ACE antibody&#44; PA2196-1&#44; IHC(P)<br>IHC(P): Human Prostatic Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2196-1-3-WB-anti-ace-cd143-antibody.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg;Anti-ACE antibody&#44; PA2196-1&#44; Western blotting<br>All lanes: Anti Angiotensin Converting Enzyme 1 (PA2196-1) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Lane 3: 22RV1 Whole Cell Lysate at 40ug<br>Predicted bind size: 150KD<br>Observed bind size: 150KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angiotensin-converting-enzyme-1-antibody-pa2196-2-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2196-2-1-WB-anti-ace-cd143-antibody.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg;Anti-ACE antibody&#44; PA2196-2&#44; Western blotting<br>All lanes: Anti ACE (PA2196-2) at 0.5ug/ml<br>Lane 1: A549 Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: 22RV1 Whole Cell Lysate at 40ug<br>Predicted bind size: 150KD<br>Observed bind size: 150KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-2a-adrenergic-receptor-antibody-pa2197-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2197-1-WB-anti-alpha-2a-adrenergic-receptor-antibody.jpgAnti-alpha 2a Adrenergic Receptor/ADRA2A Antibody Picoband&reg;Anti-alpha 2a Adrenergic Receptor antibody&#44; PA2197&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: PANC Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc6-antibody-pa2199-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2199-cdc6-primary-antibodies-wb-testing-1.jpgAnti-Cdc6 Antibody Picoband&reg; Western blot analysis of Cdc6 using anti-Cdc6 antibody (PA2199). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc6 antigen affinity purified polyclonal antibody (Catalog # PA2199) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc6 at approximately 53 kDa. The expected band size for Cdc6 is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-li-cadherin-antibody-pa2200-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2200-1-WB-anti-li-cadherin-antibody.jpgAnti-LI-Cadherin-17 CDH17-Antibody Picoband&reg;Anti-CDH17 antibody&#44; PA2200&#44; Western blotting<br>All lanes: Anti CDH17(PA2200) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: SW620 Whole Cell Lysate at 40ug<br>Predicted bind size: 92KD<br>Observed bind size: 92KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-comt-antibody-pa2203-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2203-comt-primary-antibodies-wb-testing-1.jpgAnti-Catechol O-methyltransferase COMT Antibody Picoband&reg; Western blot analysis of COMT using anti-COMT antibody (PA2203). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human SH-SY5Y whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: mouse brain tissue lysates, <br> Lane 7: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMT antigen affinity purified polyclonal antibody (Catalog # PA2203) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COMT at approximately 28 kDa. The expected band size for COMT is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2203-2-IHC-anti-comt-antibody.jpgAnti-Catechol O-methyltransferase COMT Antibody Picoband&reg; IHC analysis of COMT using anti-COMT antibody (PA2203). <br> COMT was detected in a paraffin-embedded section of Human Kidney Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-COMT Antibody (PA2203) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2203-3-IHC-anti-comt-antibody.jpgAnti-Catechol O-methyltransferase COMT Antibody Picoband&reg; IHC analysis of COMT using anti-COMT antibody (PA2203). <br> COMT was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-COMT Antibody (PA2203) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dopamine-receptor-d5-antibody-pa2204-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2204-1_1.jpgAnti-Dopamine Receptor D5/DRD5 Antibody Picoband&reg; Western blot analysis of DRD5 using anti-DRD5 antibody (PA2204). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD5 antigen affinity purified polyclonal antibody (Catalog # PA2204) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DRD5 at approximately 53KD. The expected band size for DRD5 is at 53KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-connexin-45-gja7-antibody-pa2206-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2206-1-WB-anti-connexin-45-gja7-antibody.jpgAnti-Connexin 45/GJA7/GJC1 Antibody Picoband&reg;Anti-Connexin 45/GJA7 antibody&#44; PA2206&#44; Western blotting<br>WB: MCF-7 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irakm-antibody-pa2207-1-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2207-1-1-WB-anti-irakm-antibody.jpgAnti-IRAKM/IRAK3 Antibody Picoband&reg;Anti-IRAKM antibody&#44; PA2207-1&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: JURKAT Cell Lysate<br>Lane 3: HUT102 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irf5-antibody-pa2208-boster.html2026-03-24T05:04:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2208-1-WB-anti-irf5-antibody.jpgAnti-Interferon regulatory factor 5 IRF5 Antibody Picoband&reg;Anti-IRF5 antibody&#44; PA2208&#44; Western blotting<br>Lane 1: Human Placenta Tissue Lysate<br>Lane 2: Rat Thymus Tissue Lysate<br>Lane 3: Rat Kidney Tissue Lysate<br>Lane 4: Rat Ovary Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irf8-antibody-pa2209-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2209-irf8-primary-antibodies-wb-testing-1.jpgAnti-Interferon regulatory factor 8 IRF8 Antibody Picoband&reg;Western blot analysis of IRF8 using anti-IRF8 antibody (PA2209). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF8 antigen affinity purified polyclonal antibody (PA2209) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IRF8 at approximately 48 kDa. The expected band size for IRF8 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2209-irf8-primary-antibodies-ihc-testing-3.jpgAnti-Interferon regulatory factor 8 IRF8 Antibody Picoband&reg;IHC analysis of IRF8 using anti-IRF8 antibody (PA2209). <br>IRF8 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRF8 Antibody (PA2209) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2209-irf8-primary-antibodies-ihc-testing-1.jpgAnti-Interferon regulatory factor 8 IRF8 Antibody Picoband&reg;IHC analysis of IRF8 using anti-IRF8 antibody (PA2209). <br> IRF8 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRF8 Antibody (PA2209) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2209-irf8-primary-antibodies-ihc-testing-2.jpgAnti-Interferon regulatory factor 8 IRF8 Antibody Picoband&reg;IHC analysis of IRF8 using anti-IRF8 antibody (PA2209). <br> IRF8 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRF8 Antibody (PA2209) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2209-irf8-primary-antibodies-if-testing-1.jpgAnti-Interferon regulatory factor 8 IRF8 Antibody Picoband&reg;IF analysis of IRF8 using anti-IRF8 antibody (PA2209) and anti-Tubulin Alpha antibody (M03989-3).<br> IRF8 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IRF8 Antibody (PA2209) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klf4-antibody-pa2211-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2211-1-WB-anti-klf4-gklf-antibody.jpgAnti-Krueppel-like factor 4 KLF4 Antibody Picoband&reg;Anti-KLF4 antibody&#44; PA2211&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: A549 Cell Lysate<br>Lane 3: U20S Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp24-antibody-pa2212-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2212-1-WB-anti-mmp24-mt5-mmp-antibody.jpgAnti-Matrix metalloproteinase-24 MMP24 Antibody Picoband&reg;Anti-MMP24 antibody&#44; PA2212&#44; Western blotting<br>Lane 1: PANC Cell Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: SMMC Cell Lysate<br>Lane 4: A549 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-olig1-antibody-pa2213-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2213-1-WB-anti-olig1-antibody.jpgAnti-Olig1 Antibody Picoband&reg;Anti-OLIG1 antibody&#44; PA2213&#44; Western blotting<br>All lanes: Anti OLIG1 (PA2213) at 0.5ug/ml<br>Lane 1: U87 Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 28KD<br>Observed bind size: 28KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hnf6-antibody-pa2214-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2214-onecut1-primary-antibodies-wb-testing-1.jpgAnti-HNF6/ONECUT1 Antibody Picoband&reg; Western blot analysis of ONECUT1 using anti-ONECUT1 antibody (PA2214). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human HUH7 whole cell lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: rat RH35 whole cell lysates, <br> Lane 5: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT1 antigen affinity purified polyclonal antibody (Catalog # PA2214) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT1 at approximately 55 kDa. The expected band size for ONECUT1 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pias4-antibody-pa2215-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2215-1-WB-anti-pias4-piasy-antibody.jpgAnti-E3 SUMO-protein ligase PIAS4 Antibody Picoband&reg;Anti-PIAS4 antibody&#44; PA2215&#44; Western blotting<br>All lanes: Anti PIAS4 (PA2215) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: A431 Whole Cell Lysate at 40ug<br>Predicted bind size: 57KD<br>Observed bind size: 57KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-selenium-binding-protein-1-antibody-pa2216-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2216-1-WB-anti-selenium-binding-protein-1-antibody.jpgAnti-SBP/SELENBP1 Antibody Picoband&reg;Anti-SBP/SELENBP1 antibody&#44; PA2216&#44; Western blotting<br>Lane 1: COLO320 Cell Lysate<br>Lane 2: PANC Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2216-2-IHC-anti-selenium-binding-protein-1-antibody.jpgAnti-SBP/SELENBP1 Antibody Picoband&reg;Anti-SBP/SELENBP1 antibody&#44; PA2216&#44; IHC(P)<br>IHC(P): Human Liver Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-1-antichymotrypsin-antibody-pa2217-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2217-1-WB-anti-alpha-1-antichymotrypsin-antibody.jpgAnti-AACT/SERPINA3 Antibody Picoband&reg;Anti-AACT/SERPINA3 antibody&#44; PA2217&#44; Western blotting<br>WB: SMMC Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2217-2-IHC-anti-alpha-1-antichymotrypsin-antibody.jpgAnti-AACT/SERPINA3 Antibody Picoband&reg;Anti-AACT/SERPINA3 antibody&#44; PA2217&#44; IHC(P)<br>IHC(P): Human Liver Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2217-serpina3-primary-antibodies-ihc-testing-3.jpgAnti-AACT/SERPINA3 Antibody Picoband&reg;IHC analysis of Alpha Antichymotrypsin/SERPINA3 using anti-Alpha Antichymotrypsin/SERPINA3 antibody (PA2217). <br>Alpha Antichymotrypsin/SERPINA3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Antichymotrypsin/SERPINA3 Antibody (PA2217) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2217-serpina3-primary-antibodies-ihc-testing-4.jpgAnti-AACT/SERPINA3 Antibody Picoband&reg;IHC analysis of Alpha Antichymotrypsin/SERPINA3 using anti-Alpha Antichymotrypsin/SERPINA3 antibody (PA2217). <br>Alpha Antichymotrypsin/SERPINA3 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Antichymotrypsin/SERPINA3 Antibody (PA2217) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-antithrombin-iii-antibody-pa2218-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2218-1-WB-anti-serpin-c1-antithrombin-iii-antibody.jpgAnti-Antithrombin III/SERPINC1 Antibody Picoband&reg;Anti-Antithrombin III antibody&#44; PA2218&#44; Western blotting<br>Lane 1: Rat Testis Tissue Lysate<br>Lane 2: SMMC Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br>Lane 4: RAJI Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc9a2-antibody-pa2219-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2219-1_1.jpgAnti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband&reg; Western blot analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A2 antigen affinity purified polyclonal antibody (Catalog # PA2219) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A2 at approximately 91KD. The expected band size for SLC9A2 is at 91KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2219-2-IHC-anti-slc9a2-antibody.jpgAnti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband&reg; IHC analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).<br> SLC9A2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A2 Antibody (PA2219) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2219-3-IHC-anti-slc9a2-antibody.jpgAnti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband&reg; IHC analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219).<br> SLC9A2 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC9A2 Antibody (PA2219) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2219-4_1.jpgAnti-Sodium/hydrogen exchanger 2 SLC9A2 Antibody Picoband&reg; Western blot analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse kidney tissue lysates&#44; <br> Lane 2: mouse kidney tissue lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A2 antigen affinity purified polyclonal antibody (Catalog # PA2219) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A2 at approximately 91KD. The expected band size for SLC9A2 is at 91KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sumo-1-antibody-pa2220-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2220-1-WB-anti-sumo-1-antibody.jpgAnti-Sumo 1/SUMO1 Antibody Picoband&reg;Anti-Sumo 1 antibody&#44; PA2220&#44; Western blotting<br>Lane 1: Rat Spleen Tissue Lysate<br>Lane 2: Human Placenta Tissue Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-diubiquitin-antibody-pa2222-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2222-1-WB-anti-diubiquitin-antibody.jpgAnti-Diubiquitin/UBD Antibody Picoband&reg;Anti-Diubiquitin antibody&#44; PA2222&#44; Western blotting<br>Lane 1: HELA Cell Lysate<br>Lane 2: SKOV Cell Lysate<br>Lane 3: MCF-7 Cell Lysate<br>Lane 4: A549 Cell Lysate<br>Lane 5: SMMC Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2222-3-IHC-anti-diubiquitin-antibody.jpgAnti-Diubiquitin/UBD Antibody Picoband&reg;Anti-Diubiquitin antibody&#44; PA2222&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2222-2-IHC-anti-diubiquitin-antibody.jpgAnti-Diubiquitin/UBD Antibody Picoband&reg;Anti-Diubiquitin antibody&#44; PA2222&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calponin-antibody-pa2224-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2224-1-IHC-anti-calponin-antibody.jpgAnti-Calponin/CNN1 Antibody Picoband&reg;Anti-Calponin antibody&#44; PA2224&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2224-2-WB-anti-calponin-antibody.jpgAnti-Calponin/CNN1 Antibody Picoband&reg;Anti-Calponin antibody&#44; PA2224&#44;Western blotting<br>All lanes: Anti -Calponin (PA2224) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Lane 3: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 33KD<br>Observed bind size: 33KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2224-3.jpgAnti-Calponin/CNN1 Antibody Picoband&reg; IHC analysis of Calponin using anti-Calponin antibody (PA2224). <br> Calponin was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calponin Antibody (PA2224) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2224-4.jpgAnti-Calponin/CNN1 Antibody Picoband&reg; IHC analysis of Calponin using anti-Calponin antibody (PA2224). <br> Calponin was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Calponin Antibody (PA2224) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-itga7-antibody-pa2226-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2226-1-WB-anti-itga7-integrin-alpha-7-antibody.jpgAnti-Integrin alpha-7 ITGA7 Antibody Picoband&reg;Anti-ITGA7 antibody&#44; PA2226&#44; Western blotting<br>Lane 1: 293T Cell Lysate<br>Lane 2: A431 Cell Lysate<br>Lane 3: HELA Cell Lysate<br>Lane 4: JURKAT Cell Lysate<br>Lane 5: RAJI Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pltp-antibody-pa2228-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2228-1-IHC-anti-pltp-antibody.jpgAnti-Phospholipid transfer protein PLTP Antibody Picoband&reg;Anti-PLTP antibody&#44; PA2228&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2228-2-WB-anti-pltp-antibody.jpgAnti-Phospholipid transfer protein PLTP Antibody Picoband&reg;Anti-PLTP antibody&#44; PA2228&#44;Western blotting<br>All lanes: Anti PLTP (PA2228) at 0.5ug/ml<br>Lane 1: MCF-7 Whole Cell Lysate at 40ug<br>Lane 2: RAJI Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 55KD<br>Observed bind size: 55KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prox1-antibody-pa2229-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2229-1-WB-anti-prox1-antibody.jpgAnti-Prospero homeobox protein 1 PROX1 Antibody Picoband&reg;Anti-PROX1 antibody&#44; PA2229&#44; Western blotting<br>Lane 1: Rat Thymus Tissue Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: JURKAT Cell Lysate<br>Lane 4: MM231 Cell Lysate<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cortisol-binding-globulin-antibody-pa2230-boster.html2026-03-24T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2230-serpina6-primary-antibodies-wb-testing-1.jpgAnti-Cortisol Binding Globulin/SERPINA6 Antibody Picoband&reg; Western blot analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (PA2230). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HL-60 whole cell lysates, <br> Lane 2: human A375 whole cell lysates, <br> Lane 3: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cortisol Binding Globulin antigen affinity purified polyclonal antibody (Catalog # PA2230) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cortisol Binding Globulin at approximately 60 kDa. The expected band size for Cortisol Binding Globulin is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2230-2-IF-anti-cortisol-binding-globulin-antibody.jpgAnti-Cortisol Binding Globulin/SERPINA6 Antibody Picoband&reg; ICC analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (PA2230). <br> Cortisol Binding Globulin was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-Cortisol Binding Globulin Antibody (PA2230) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2230-3.jpgAnti-Cortisol Binding Globulin/SERPINA6 Antibody Picoband&reg; IHC analysis of Cortisol Binding Globulin using anti-Cortisol Binding Globulin antibody (PA2230). <br> Cortisol Binding Globulin was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cortisol Binding Globulin Antibody (PA2230) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aif-antibody-pa2232-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2232-aifm1-primary-antibodies-wb-testing-1.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; Western blot analysis of AIFM1 using anti-AIFM1 antibody (PA2232). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat kidney tissue lysates,<br> Lane 6: rat spleen tissue lysates,<br> Lane 7: mouse kidney tissue lysates,<br> Lane 8: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIFM1 antigen affinity purified polyclonal antibody (Catalog # PA2232) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIFM1 at approximately 67 kDa. The expected band size for AIFM1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2232-1-IHC-anti-aif-antibody.jpgAnti-AIF/AIFM1 Antibody Picoband&reg;Anti-AIF antibody&#44; PA2232&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2232-2-IHC-anti-aif-antibody.jpgAnti-AIF/AIFM1 Antibody Picoband&reg;Anti-AIF antibody&#44; PA2232&#44; IHC(P)<br>IHC(P): Rat Cardiac Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2232-aifm1-primary-antibodies-ihc-testing-4.jpgAnti-AIF/AIFM1 Antibody Picoband&reg;IHC analysis of AIF/AIFM1 using anti-AIF/AIFM1 antibody (PA2232). <br>AIF/AIFM1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AIF/AIFM1 Antibody (PA2232) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2232-aifm1-primary-antibodies-if-testing-4.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; IF analysis of AIFM1 using anti-AIFM1 antibody (PA2232). <br> AIFM1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AIFM1 Antibody (PA2232) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-g-protein-coupled-receptor-30-antibody-pa2235-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2235-1-WB-anti-g-protein-coupled-receptor-30-antibody.jpgAnti-G-protein coupled receptor 30/GPER1 Antibody Picoband&reg;Anti-G-protein coupled receptor 30 antibody&#44; PA2235&#44; Western blotting<br>Lane 1: COLO320 Cell Lysate<br>Lane 2: MCF-7 Cell Lysate<br>Lane 3: COS7 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irak2-antibody-pa2236-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2236-1-WB-anti-irak2-antibody.jpgAnti-IRAK2 Antibody Picoband&reg;Anti-IRAK2 antibody&#44; PA2236&#44; Western blotting<br>Lane 1: 22RV Cell Lysate<br>Lane 2: A549 Cell Lysate<br>Lane 3: PANC Cell Lysate<br>Lane 4: SMMC Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2236-2-IHC-anti-irak2-antibody.jpgAnti-IRAK2 Antibody Picoband&reg;Anti-IRAK2 antibody&#44; PA2236&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2236-3-IHC-anti-irak2-antibody.jpgAnti-IRAK2 Antibody Picoband&reg;Anti-IRAK2 antibody&#44; PA2236&#44; IHC(P)<br>IHC(P): Rat Spleen Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kin-antibody-pa2238-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2238-1-WB-anti-kin-kin17-antibody.jpgAnti-DNA/RNA-binding protein KIN17 KIN Antibody Picoband&reg;Anti-KIN antibody&#44; PA2238&#44; Western blotting<br>Lane 1: Rat Skeletal Muscle Tissue Lysate<br>Lane 2: Human Placenta Tissue Lysate<br>Lane 3: Rat Testis Tissue Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-securin-antibody-pa2240-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2240-1_1-WB-anti-securin-antibody.jpgAnti-Securin/PTTG1 Antibody Picoband&reg;Anti-Securin antibody&#44; PA2240&#44; Western blotting<br>All lanes: Anti PTTG1 (PA2240) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Lane 3: SKOV Whole Cell Lysate at 40ug<br>Lane 4: A375 Whole Cell Lysate at 40ug<br>Predicted bind size: 22KD<br>Observed bind size: 22KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rgs9-antibody-pa2241-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2241-1-WB-anti-rgs9-antibody.jpgAnti-RGS9 Antibody Picoband&reg;Anti-RGS9 antibody&#44; PA2241&#44; Western blotting<br>Lane 1: Rat Brain Tissue Lysate<br>Lane 2: Mouse Brain Tissue Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2241-2.jpgAnti-RGS9 Antibody Picoband&reg; Western blot analysis of RGS9 using anti-RGS9 antibody (PA2241). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PANC-1 whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS9 antigen affinity purified polyclonal antibody (Catalog # PA2241) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for RGS9 at approximately 56&#44; 77KD. The expected band size for RGS9 is at 77KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rip3-antibody-pa2242-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2242-1-WB-anti-rip3-antibody.jpgAnti-RIP3/RIPK3 Antibody Picoband&reg;Anti-RIP3 antibody&#44; PA2242&#44; Western blotting<br>Lane 1: PANC Cell Lysate<br>Lane 2: SW620 Cell Lysate<br>Lane 3: SKOV-3 Cell Lysate<br>Lane 4: M231 Cell Lysatehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2242-thnov09p8277g002.jpgAnti-RIP3/RIPK3 Antibody Picoband&reg;Conditional knockout of TGF-βRII prevented cisplatin-induced renal injury and apoptotic signaling in vivo . A: Periodic acid-Schiff (PAS) staining and quantitative analysis show conditional knockout of TGF-βRII reduced renal injury in cisplatin nephropathy. B: Creatinine assay. C: BUN assay. Serum creatinine and BUN show conditional knockout of TGF-βRII prevented decline of renal function in cisplatin nephropathy. D. Real-time PCR data show conditional knockout of TGF-βRII reduced TGF-β mRNA level in cisplatin-induced nephropathy. E. Western blot analysis of phospho-Smad2 and phospho-Smad3. F. Immunohistochemistry and quantitative data show conditional knockout of TGF-βRII reduced KIM-1 protein and F4/80+ macrophages infiltration in cisplatin nephropathy. G. Western blot analysis of KIM-1, RIPK1, RIPK3, cleaved caspase-3. Data represent mean ± SEM for 6-8 mice. **P<0.01, ***P<0.001 versus normal; ## P<0.01, ### P<0.001 versus TGF-βRII FF+ cisplatin group. TGF-βRII FF: TGF-βRII flox/flox mouse; TGF-βRII KspCre: conditional TGF-βRII knockout mice; Magnification: 100X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6857044/&orig_host=www.ncbi.nlm.nih.gov'>31754396</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2242-thnov09p8277g003.jpgAnti-RIP3/RIPK3 Antibody Picoband&reg;Conditional knockout of Smad2 prevented cisplatin-induced renal injury, decline of renal function and attenuated signaling molecules regulating programmed cell death in vivo . A: PAS staining and quantitative analysis show conditional knockout of Smad2 reduced renal injury in cisplatin-induced AKI mice. B: Creatinine assay. C: BUN assay. Serum creatinine and BUN show conditional deletion of Smad2 prevented decline of renal function in cisplatin nephropathy. D: Immunohistochemistry and quantitative data show conditional knockout of Smad2 reduced KIM-1 in cisplatin-induced nephropathy. E and F: Western blot and Real-time PCR analysis of KIM-1. G: Western blot of P-p53, p53, RIPK1, RIPK3 and cleaved caspase-3. Data represent mean ± SEM for 6-8 mice. **P<0.01, ***P<0.001 versus normal; # P<0.05, ## P<0.01, ### P<0.001 versus Smad2FF+cisplatin group. S2FF: Smad2 flox/flox mouse; S2 KspCre: conditional Smad2 knockout mice; KIM-1: kidney injury molecule-1. Magnification: 100X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6857044/&orig_host=www.ncbi.nlm.nih.gov'>31754396</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2242-thnov09p8277g007.jpgAnti-RIP3/RIPK3 Antibody Picoband&reg;Knockdown of Smad2 attenuates cisplatin-induced renal injury, inflammation response, and programmed cell death in established nephrotoxic AKI model. A: PAS staining and quantitative analysis show knockdown of Smad2 reduced renal injury in cisplatin nephropathy. B: Creatinine assay. C: BUN assay. Serum creatinine and BUN show knockdown of Smad2 prevented decline of renal function in cisplatin nephropathy. D: Real-time PCR data show knockdown of Smad2 reduced mRNA of TNF-α, IL-1β, MCP-1 and KIM-1. E: Immunohistochemistry and quantitative data show knockdown of Smad2 reduced KIM-1 protein and F4/80+ macrophages in cisplatin-induced nephropathy. F: TUNEL assay. Knockdown of Smad2 reduced apoptosis in injured kidney. G: Western blot analysis of KIM-1, RIPK1, RIPK3, cleaved caspase-3. Data represent mean ± SEM for 6 mice. *P<0.05, **P<0.01, ***P<0.001 versus control; # P<0.05, ## P<0.01, ### P<0.001 versus Smad2 vector control+cisplatin. KD: knockdown. Magnification: 100X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6857044/&orig_host=www.ncbi.nlm.nih.gov'>31754396</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2242-thnov09p8277g008.jpgAnti-RIP3/RIPK3 Antibody Picoband&reg;Knockdown of Smad2 attenuates renal injury, inflammation response, and programmed cell death in established ischemic AKI model. A: PAS staining and quantitative analysis show knockdown of Smad2 reduced renal injury in I/R-induced AKI model. B: Creatinine assay. C: BUN assay. Serum creatinine and BUN show knockdown of Smad2 prevented decline of renal function. D: Real-time PCR data show knockdown of Smad2 reduced mRNA of TNF-α, IL-1β, MCP-1 and KIM-1. E: Immunohistochemistry and quantitative data show knockdown of Smad2 reduced KIM-1 protein and F4/80+ macrophages infiltration in injured kidney. F: TUNEL assay. G: Western blot analysis of KIM-1, RIPK1, RIPK3, cleaved caspase-3. Data represent mean ± SEM for 6 mice. *P<0.05, **P<0.01, ***P<0.001 versus control; # P<0.05, ## P<0.01, ### P<0.001 versus Smad2 vector control+IRI. KD: knockdown. Magnification: 100X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6857044/&orig_host=www.ncbi.nlm.nih.gov'>31754396</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sglt1-antibody-pa2244-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2244-slc5a1-primary-antibodies-wb-testing-1.jpgAnti-SGLT1/SLC5A1 Antibody Picoband&reg; Western blot analysis of SLC5A1 using anti-SLC5A1 antibody (PA2244). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: rat NRK whole cell lysates,<br> Lane 5: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A1 antigen affinity purified polyclonal antibody (Catalog # PA2244) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A1 at approximately 73 kDa. The expected band size for SLC5A1 is at 73 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cat1-antibody-pa2245-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2245-1-WB-anti-cat1-antibody.jpgAnti-CAT1/SLC7A1 Antibody Picoband&reg;Anti-CAT1 antibody&#44; PA2245&#44; Western blotting<br>Lane 1: Human Placenta Tissue Lysate<br>Lane 2: HELA Cell Lysate<br>Lane 3: SKOV-3 Cell Lysate<br>Lane 4: HT1080 Cell Lysate https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr1-antibody-pa2246-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2246-1-WB-anti-tlr1-antibody.jpgAnti-Toll-like receptor 1 TLR1 Antibody Picoband&reg;Anti-TLR1 antibody&#44; PA2246&#44; Western blotting<br>All lanes: Anti TLR1 (PA2246) at 0.5ug/ml<br>Lane 1: COLO320 Whole Cell Lysate at 40ug<br>Lane 2: SW620 Whole Cell Lysate at 40ug<br>Lane 3: SKOV Whole Cell Lysate at 40ug<br>Lane 4: JURKAT Whole Cell Lysate at 40ug<br>Lane 5: CEM Whole Cell Lysate at 40ug<br>Lane 6: PANC Whole Cell Lysate at 40ug<br>Predicted bind size: 90KD<br>Observed bind size: 90KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-avpr1a-antibody-pa2248-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2248-1-WB-anti-avpr1a-antibody.jpgAnti-Vasopressin V1a receptor AVPR1A Antibody Picoband&reg;Anti-AVPR1A antibody&#44; PA2248&#44; All Western blotting<br>All lanes: Anti-AVPR1A(PA2248) at 0.5ug/ml <br>Lane 1: Rat Liver Tissue Lysate at 40ug <br>Lane 2: Rat Kidney Tissue Lysate at 40ug <br>Predicted bind size: 47KD <br>Observed bind size: 47KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2248-10020_2025_1272_fig2_html.pngAnti-Vasopressin V1a receptor AVPR1A Antibody Picoband&reg;RNA-seq reveals key molecular signatures in hippocampal tissue of DHEA-treated mice. A Cluster heatmap of differentially expressed genes between the control and DHEA-treated groups. B The total GO term analysis (Top 20) revealed that hormone activity was prioritized. C The total KEGG enrichment analysis (Top 20) showed that neuroactive ligand-receptor interaction was significantly enriched. D KEGG enrichment analysis using chord diagrams revealed several genes, such as Avpr1a, in the neuroactive ligand-receptor interaction pathway <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01272-9'>40437391</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2248-10020_2025_1272_fig3_html.pngAnti-Vasopressin V1a receptor AVPR1A Antibody Picoband&reg;Expression of candidate differential genes was verified by qRT-PCR in female mouse hippocampal tissues. A Candidate genes and the signaling pathways they are involved in. B-G mRNA expression levels of Aox2 , Pomc , Wnt3a , Avpr1a , Ccr10 , and P2ry10b as determined by qRT-PCR ( n = 9). The data are shown as the mean ± SEM. An unpaired Student’s t-test was used for statistical analysis, and the experiment was repeated three times independently with similar results. * indicates p < 0.05, ** indicates p < 0.01, **** indicates p < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01272-9'>40437391</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2248-10020_2025_1272_fig4_html.pngAnti-Vasopressin V1a receptor AVPR1A Antibody Picoband&reg;DHEA decreased the activity of the AVP system in the hippocampus. A Protein levels of AVPR1a in the hippocampal tissue detected by western blot (DHEA-treated vs. Control, n = 3). B Relative quantification of AVPR1a protein in the hippocampus of female mice in the DHEA-treated ( n = 3) and control ( n = 3) groups. C-D The content of AVP and OT in the hippocampus tissues of female mice in the DHEA-treated ( n = 7–8) and control ( n = 7–8) groups, as determined by ELISA. The data are shown as the mean ± SEM. An unpaired Student’s t-test was used for statistical analysis, and the experiment was repeated three times independently with similar results, * indicates p < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01272-9'>40437391</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2248-10020_2025_1272_fig5_html.pngAnti-Vasopressin V1a receptor AVPR1A Antibody Picoband&reg;DHEA regulates Avpr1a transcription directly through AR. A Experimental design for ChIP-PCR. The yellow fan indicates the androgen receptor (AR). The long green line represents the Avpr1a DNA. The red rectangle indicates the binding site of the androgen to the Avpr1a promoter. B Electropherogram of a ChIP-PCR experiment. The bands in the AR group were more intense and more specific than those in the IgG group using the two primers in the PCR process. C Sequences of the primers for ChIP-PCR. The experiment was repeated three times independently with similar results <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01272-9'>40437391</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2248-10020_2025_1272_fig6_html.pngAnti-Vasopressin V1a receptor AVPR1A Antibody Picoband&reg;DHEA inhibited AVPR1a expression in HT22 cells via AR. A The expression of AVPR1a protein in HT22 cells treated with DHEA at different concentrations (0 µM, 10 µM and 20 µM) for different durations (24 h and 48 h) was detected by western blot. B , C The relative expression of AVPR1a protein ( B ) and mRNA ( C ) in HT22 cells treated with different concentrations (0 µM, 10 µM, and 20 µM) of DHEA for different periods (24 h and 48 h). D The expression of AVPR1a protein in DHEA-treated HT22 cells after antagonist pretreatment was detected by western blot. E , F The relative expression of AR protein ( E ) and mRNA ( F ) in DHEA-treated HT22 cells after antagonist pretreatment. G , H The relative expression of AVPR1a protein ( G ) and mRNA ( H ) in DHEA-treated HT22 cells after antagonist pretreatment. The data are shown as the mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc test was used for Fig. 6B-C and one-way ANOVA followed by Tukey’s post hoc test was used for Fig. 6E-F, G-H, and the experiment was repeated three times independently with similar results. * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, ns indicates no significant difference <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01272-9'>40437391</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gria3-antibody-pa2249-boster.html2026-03-24T05:04:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2249-1-WB-anti-gria3-glur-3-antibody.jpgAnti-Glutamate receptor 3/GRIA3 Antibody Picoband&reg;Anti-GRIA3 antibody&#44; PA2249&#44; All Western blotting<br>All lanes: Anti-GRIA3 (PA2249) at 0.5ug/ml <br>Lane 1: Rat Brain Tissue Lysate at 40ug <br>Lane 2: Mouse Brain Tissue Lysate at 40ug <br>Predicted bind size: 101KD <br>Observed bind size: 101KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ncstn-antibody-pa2250-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2250-ncstn-primary-antibodies-wb-testing-1.jpgAnti-Nicastrin/NCSTN Antibody Picoband&reg; Western blot analysis of NCSTN using anti-NCSTN antibody (PA2250). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCSTN antigen affinity purified polyclonal antibody (Catalog # PA2250) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCSTN at approximately 120-130 kDa. The expected band size for NCSTN is at 78 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pias1-antibody-pa2252-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2252-1-IF-anti-pias1-antibody.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband&reg;Anti-PIAS1 antibody&#44; PA2252&#44; ICC<br>ICC: A549 Cellhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2252-2-IHC-anti-pias1-antibody.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband&reg;Anti-PIAS1 antibody&#44; PA2252&#44; IHC(P)<br>IHC(P): Rat Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2252-3-IHC-anti-pias1-antibody.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband&reg;Anti-PIAS1 antibody&#44; PA2252&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2252-4-IHC-anti-pias1-antibody.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband&reg;Anti-PIAS1 antibody&#44; PA2252&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2252-5-IHC-anti-pias1-antibody.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband&reg;Anti-PIAS1 antibody&#44; PA2252&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2252-6-WB-anti-pias1-antibody.jpgAnti-E3 SUMO-protein ligase PIAS1 PIAS1 Antibody Picoband&reg;Anti-PIAS1 antibody&#44; PA2252&#44; All Western blotting<br>All lanes: Anti-PIAS1(PA2252) at 0.5ug/ml <br>Lane 1: Rat Testis Tissue Lysate at 40ug <br>Lane 2: Mouse Testis Tissue Lysate at 40ug <br>Lane 3: HELA Whole Cell Lysate at 40ug <br>Lane 4: JURKAT Whole Cell Lysate at 40ug <br>Lane 5: MCF-7 Whole Cell Lysate at 40ug <br>Lane 6: SKOV Whole Cell Lysate at 40ug <br>Predicted bind size: 72KD <br>Observed bind size: 100KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pon1-antibody-pa2253-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2253-1-WB-anti-pon1-paraoxonase-1-antibody.jpgAnti-PON1 Antibody Picoband&reg;Anti-PON1 antibody&#44; PA2253&#44; All Western blotting<br>All lanes: Anti-PON1(PA2253) at 0.5ug/ml <br>Lane 1: Rat Liver Tissue Lysate at 40ug <br>Lane 2: Rat Lung Tissue Lysate at 40ug <br>Lane 3: Human Placenta Tissue Lysate at 40ug <br>Lane 4: Rat Testis Tissue Lysate at 40ug <br>Lane 5: HELA Whole Cell Lysate at 40ug <br>Lane 6: HEPA Whole Cell Lysate at 40ug <br>Lane 7: A549 Whole Cell Lysate at 40ug <br>Lane 8: JURKAT Whole Cell Lysate at 40ug <br>Lane 9: SKOV Whole Cell Lysate at 40ug <br>Predicted bind size: 40KD <br>Observed bind size: 40KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnfaip8l3-antibody-pa2257-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2257-1-IHC-anti-tnfaip8l3-antibody.jpgAnti-TNFAIP8L3 Antibody Picoband&reg;Anti-TNFAIP8L3 antibody&#44; PA2257&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2257-2-IHC-anti-tnfaip8l3-antibody.jpgAnti-TNFAIP8L3 Antibody Picoband&reg;Anti-TNFAIP8L3 antibody&#44; PA2257&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2257-3-WB-anti-tnfaip8l3-antibody.jpgAnti-TNFAIP8L3 Antibody Picoband&reg;Anti-TNFAIP8L3 antibody&#44; PA2257&#44; All Western blotting<br>All lanes: Anti-TNFAIP8L3(PA2257) at 0.5ug/ml <br>Lane 1: MCF-7 Whole Cell Lysate at 40ug <br>Lane 2: SW620 Whole Cell Lysate at 40ug <br>Lane 3: COS7 Whole Cell Lysate at 40ug <br>Lane 4: SKOV Whole Cell Lysate at 40ug <br>Lane 5: JURKAT Whole Cell Lysate at 40ug <br>Predicted bind size: 33KD <br>Observed bind size: 33KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ube2i-ubc9-antibody-pa2258-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2258-ube2i-primary-antibodies-wb-testing-1.jpgAnti-UBE2I/UBC9 Antibody Picoband&reg;Western blot analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (PA2258). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2I/UBC9 antigen affinity purified polyclonal antibody (PA2258) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2I/UBC9 at approximately 18 kDa. The expected band size for UBE2I/UBC9 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2258-ube2i-primary-antibodies-ip-testing-1.jpgAnti-UBE2I/UBC9 Antibody Picoband&reg;Immunoprecipitating (IP) UBE2I/UBC9 in HepG2 whole cell lysate.<br> Western blot analysis of UBE2I/UBC9 using anti-UBE2I/UBC9 antibody (PA2258); <br> Lane 1: HepG2 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-UBE2I/UBC9 antibody in HepG2 whole cell lysate;<br> Lane 3: anti-UBE2I/UBC9 antibody (2μg) + HepG2 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-UBE2I/UBC9 antigen affinity purified polyclonal antibody (PA2258) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for UBE2I/UBC9 at approximately 18 kDa. The expected band size for UBE2I/UBC9 is at 18 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apg5l-atg5-antibody-pa2260-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-atg5-primary-antibodies-wb-testing-1_1.jpgAnti-APG5L/ATG5 Antibody Picoband&reg;Western blot analysis of ATG5 using anti-ATG5 antibody (PA2260). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br>Lane 1: Lane 1: human A549 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG5 antigen affinity purified polyclonal antibody (PA2260) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATG5 at approximately 50-52 kDa. The expected band size for ATG5 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-atg5-primary-antibodies-ihc-testing-1.jpgAnti-APG5L/ATG5 Antibody Picoband&reg;IHC analysis of ATG5 using anti-ATG5 antibody (PA2260). <br>ATG5 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATG5 Antibody (PA2260) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-41419_2020_2930_fig5_html.pngAnti-APG5L/ATG5 Antibody Picoband&reg;Hypoxia leads to the transfer of ZNF423 from the nucleus to the cytoplasm, where it bound BCAT1 to promote autophagy activity. a Bioinformatics analysis of proteins associated with BCAT1. Upside: According to the JASPAR database and LASAGNA-Search 2.0 database, there was 28 genes that may bind to bcat1, and the binding ability of ZNF423, STAT1, Pou5f1, STAT3, SP1, SOX9, and TEAD1 was strong. Underside: RT-PCR analysis of the mRNA levels of ZNF423, STAT1, Pou5f1, STAT3, SP1, SOX9, and TEAD1 with rat β-actin serving as the standard in PASMCs under NOR or HYP for 24 h ( n = 5). b Western blot analysis of the expression of ZNF423 in PASMCs under NOR or HYP for 24 h ( n = 6). c ZNF423 protein levels were assayed in pulmonary arterial tissues of hypoxic model rats ( n = 4). d Coimmunoprecipitation of whole-cell lysates of PASMCs exposed to normoxia or hypoxia for 24 h with anti-ZNF423, followed by probing with anti-BCAT1 ( n = 3). e Western blot analysis of BCAT1 expression in PASMCs transfected with ZNF423 siRNA under NOR or HYP for 24 h ( n = 4). f PASMCs were exposed to HYP for 24 h, and the colocalization between BCAT1 and ZNF423 was determined by immunofluorescence. GFP-BCAT1 (green), ZNF423 (red), and DAPI (blue). Scale bar = 50 μm ( n = 3). g The translocation of ZNF423 between the nucleus and cytoplasm in PASMCs transfected with BCAT1 siRNA or gabapentin ( n = 3). h Western blot analysis of the expression of BECN1 and Atg5 in PASMCs transfected with ZNF423 siRNA under HYP for 24 h ( n = 4). i Autophagic flux of PASMCs cotransfected with eGFP-mRFP-LC3 plasmid and control siRNA or ZNF423 siRNA under HYP for 24 h. Scale bar = 50 μm ( n = 5). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, H + si-ZNF423 hypoxia plus ZNF423 siRNA, IP immunoprecipitation, IB immunoblotting. Statistical analysis was performed with one-way ANOVA or the Student’s t test. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-41419_2020_2930_fig4_html.pngAnti-APG5L/ATG5 Antibody Picoband&reg;BCAT1 regulates autophagy during hypoxia by activating ERs via the IRE1-XBP1-RIDD axis. a Western blot analysis of BECN1 and Atg5 in PASMCs cotransfected with BCAT1 and IRE1 siRNA ( n = 5). b Autophagic flux was monitored in PASMCs cotransfected with eGFP-mRFP-LC3 plasmid and control siRNA or IRE1 siRNA that were then exposed to HYP for 24 h. Scale bar = 50 μm ( n = 3). c , d RT-PCR analysis of the mRNA levels of XBP1-s, sparc, pmp2, and Scara3 with rat β-actin serving as the standard ( n = 5). e The formation of autophagosomes was detected, and autophagic activity was estimated in cells in which the expression of XBP1 was knocked down with XBP1 siRNA under HYP for 24 h. Scale bar = 50 µm ( n = 5). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, H + SI-IRE1 hypoxia plus IRE1 siRNA, H + SI-XBP1 hypoxia plus XBP1 siRNA, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid, H + Con+NC hypoxia plus control vector plus control siRNA, H + B + Si-IRE hypoxia plus BCAT1 plasmid plus IRE1 siRNA. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-41419_2020_2930_fig3_html.pngAnti-APG5L/ATG5 Antibody Picoband&reg;BCAT1 regulates autophagy through the endoplasmic reticulum stress pathway. a Expression of BCAT1 and ER-Tracker Red staining in PASMCs exposed to NOR or HYP for 24 h. Scale bar = 50 μm ( n = 3). b Western blot analysis of PERK, IRE1, ATF6, and GRP78 protein expression in the ERs pathway in PASMCs treated with gabapentin ( n = 8). c Western blot analysis of IRE1, PERK, ATF6, and GRP78 expression in PASMCs transfected with BCAT1 siRNA ( n = 8). d Western blot analysis of BECN1 and Atg5 in PASMCs treated with the ERs pathway inhibitor 4-PBA and BCAT1 plasmid ( n = 8). e Coimmunoprecipitation of the whole-cell lysates of PASMCs exposed to normoxia or hypoxia for 24 h with anti-IRE1, followed by probing with anti-BCAT1 ( n = 3). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, N + Con normoxia plus control vector, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid, H + Con+4 hypoxia plus control vector plus 4-phenylbutyric acid, H + B + 4 hypoxia plus BCAT1 plasmid plus 4-phenylbutyric acid, IP immunoprecipitation, IB immunoblotting. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-41419_2022_5037_fig2_html.pngAnti-APG5L/ATG5 Antibody Picoband&reg;Iron-overloaded EMFF induced ferritinophagy-dependent ferroptosis in granulosa cells. A – C Levels of total iron, hepcidin, and transferrin in EMFF ( n = 15) and COFF ( n = 15). Data are expressed as means ± SD and analyzed by Student’s t test. D Results of mouse granulosa cells proliferation under different intervention conditions (each group in the figure is compared with COFF group). DFO, iron chelators; FER, ferroptosis inhibitor; NEC, necrosis inhibitor; ZDF, apoptosis inhibitor; ME, autophagy inhibitor. Data are expressed as means ± SD and analyzed by one-way ANOVA. E – H Comparison of ferritinophagy-related proteins FTH1, NCOA4, and ATG5 between human granulosa cells of infertile patients with EMs (EMGC) and of control group (COGC). The expression of β-actin was used as an internal control. Data are expressed as means ± SD and analyzed by Student’s t test. I – L Detection of ferroptosis-related indicators iron, GSH, GPX4, and MDA in COGC and EMGC. Data are expressed as means ± SD and analyzed by Student’s t test. M Representative images of the mitochondrial morphology of mouse granulosa cells intervened by COFF and EMFF were observed under TEM. Yellow arrows indicate mitochondrion. Scale bar = 1.0 µm. Scale bar = 5.0 µm. N Representative images of ROS and ferrous ion fluorescence staining after COFF and EMFF intervention in mouse granulosa cells. Scale bar = 100 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-022-05037-8'>35787614</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-41419_2020_2930_fig2_html.pngAnti-APG5L/ATG5 Antibody Picoband&reg;Upregulation of BCAT1 expression induced by hypoxia leads to PASMC autophagy. a Western blot analysis of BECN1 and Atg5 protein expression in PASMCs treated with the inhibitor gabapentin (20 µM) ( n = 8). b Western blot analysis of BECN1 and Atg5 protein expression in PASMCs transfected with BCAT1 siRNA or BCAT1 plasmid ( n = 8). c , d Immunofluorescence staining for BECN1 and Atg5 in PASMCs. BECN1 and Atg5 (green), α-SMA (red), and DAPI (blue). Scale bar = 50 μm ( n = 3). e Western blot analysis of BECN1 and Atg5 expression in the pulmonary arterial tissues of hypoxia model rats treated with gabapentin ( n = 7). f Measurement of autophagic flux in PASMCs transfected with eGFP-mRFP-LC3 plasmid and exposed under NOR or HYP for 24 h treated with BCAT1 siRNA or the BCAT1 inhibitor gabapentin. Yellow and red dots indicate autolysosomes and autophagosomes, respectively. Scale bar = 50 μm ( n = 6). Nor normoxia, Hyp hypoxia, Mct monocrotaline, H + G hypoxia plus gabapentin, M + G monocrotaline plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-41419_2022_5037_fig5_html.pngAnti-APG5L/ATG5 Antibody Picoband&reg;Construction of an iron overload mouse model. A – C Serum levels of E 2 , FSH, and LH in standard iron (STD), low iron (LID), and high iron (HID) diet feeding groups ( n = 8). D – F Total iron, GSH, and MDA levels in the ovary tissues of mice in each group ( n = 8). G Representative images of ROS fluorescence staining of ovarian mouse granulosa cells in three groups of mice. Scale bar = 20 µm. H – K Western blot analysis of ferritinophagy-related proteins, FTH1, NCOA4, and ATG5 in mouse ovary tissues in the STD, LID, and HID group. The expression of β-actin was used as an internal control. All data are expressed as means ± SD and analyzed by one-way ANOVA. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns, no significance. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-022-05037-8'>35787614</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-atg5-primary-antibodies-if-testing-1.jpgAnti-APG5L/ATG5 Antibody Picoband&reg;IF analysis of ATG5 using anti-ATG5 antibody (PA2260). <br> ATG5 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG5 Antibody (PA2260) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2260-atg5-primary-antibodies-fcm-testing-1.jpgAnti-APG5L/ATG5 Antibody Picoband&reg;Flow Cytometry analysis of HepG2 cells using anti-ATG5 antibody (PA2260). <br>Overlay histogram showing CACO-2 cells stained with PA2260 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG5 Antibody (PA2260, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apg7-antibody-pa2261-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2261-atg7-primary-antibodies-wb-testing-1.jpgAnti-Apg7/ATG7 Antibody Picoband&reg; Western blot analysis of Apg7/ATG7 using anti-Apg7/ATG7 antibody (PA2261). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates, <br> Lane 5: rat kidney tissue lysates, <br> Lane 6: mouse kidney tissue lysates, <br> Lane 7: mouse spleen tissue lysates, <br> Lane 8: mouse SP2/0 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg7/ATG7 antigen affinity purified polyclonal antibody (Catalog # PA2261) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apg7/ATG7 at approximately 78 kDa. The expected band size for Apg7/ATG7 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2261-atg7-primary-antibodies-fcm-testing-2.jpgAnti-Apg7/ATG7 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-Apg7/ATG7 antibody (PA2261). <br>Overlay histogram showing Hela cells stained with PA2261 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Apg7/ATG7 Antibody (PA2261, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prmt4-antibody-pa2263-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2263-carm1-primary-antibodies-wb-testing-1.jpgAnti-PRMT4/CARM1 Antibody Picoband&reg; Western blot analysis of PRMT4/CARM1 using anti-PRMT4/CARM1 antibody (PA2263). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: rat C6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRMT4/CARM1 antigen affinity purified polyclonal antibody (Catalog # PA2263) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRMT4/CARM1 at approximately 66 kDa. The expected band size for PRMT4/CARM1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2263-carm1-primary-antibodies-if-testing-2.jpgAnti-PRMT4/CARM1 Antibody Picoband&reg; IF analysis of PRMT4/CARM1 using anti-PRMT4/CARM1 antibody (PA2263). <br> PRMT4/CARM1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PRMT4/CARM1 Antibody (PA2263) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2263-carm1-primary-antibodies-fcm-testing-3.jpgAnti-PRMT4/CARM1 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-PRMT4/CARM1 antibody (PA2263). <br> Overlay histogram showing 293T cells stained with PA2263 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT4/CARM1 Antibody (PA2263, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-epb41l1-antibody-pa2266-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2266-epb41l1-primary-antibodies-wb-testing-1.jpgAnti-Band 4.1-like protein 1 EPB41L1 Antibody Picoband&reg;Western blot analysis of EPB41L1 using anti-EPB41L1 antibody (PA2266). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hacat whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human SH-SY5Y whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPB41L1 antigen affinity purified polyclonal antibody (PA2266) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPB41L1 at approximately 100-130 kDa. The expected band size for EPB41L1 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2266-epb41l1-primary-antibodies-wb-testing-2.jpgAnti-Band 4.1-like protein 1 EPB41L1 Antibody Picoband&reg;Western blot analysis of EPB41L1 using anti-EPB41L1 antibody (PA2266). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPB41L1 antigen affinity purified polyclonal antibody (PA2266) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for EPB41L1 at approximately 100-130 kDa. The expected band size for EPB41L1 is at 99 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hnf1-beta-antibody-pa2267-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2267-2-WB-anti-hnf1-beta-antibody.jpgAnti-HNF1 beta/HNF1B Antibody Picoband&reg;Anti-HNF1 beta antibody&#44; PA2267&#44; All Western blotting<br>All lanes: Anti-HNF1B(PA2267) at 0.5ug/ml<br> Lane 1: Mouse Liver Tissue Lysate at 40ug <br>Predicted bind size: 61KD <br>Observed bind size: 61KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-huntingtin-antibody-pa2268-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2268-1-WB-anti-huntingtin-antibody.jpgAnti-Huntingtin/HTT Antibody Picoband&reg;Anti-Huntingtin antibody&#44; PA2268&#44; All Western blotting<br>All lanes: Anti-HTT(PA2268) at 0.5ug/ml <br>Lane 1: HELA Whole Cell Lysate at 40ug <br>Lane 2: U87 Whole Cell Lysate at 40ug <br>Predicted bind size: 348KD <br>Observed bind size: 348KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irs1-antibody-pa2269-boster.html2026-03-24T05:04:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2269-1-WB-anti-irs1-antibody.jpgAnti-Insulin receptor substrate 1 IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PA2269&#44; All Western blotting<br>All lanes: Anti-IRS1(PA2269) at 0.5ug/ml <br>Lane 1: HELA Whole Cell Lysate at 40ug <br>Lane 2: A549 Whole Cell Lysate at 40ug <br>Lane 3: 293T Whole Cell Lysate at 40ug <br>Lane 4: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 132KD<br> Observed bind size: 132KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lrp5-antibody-pa2273-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2273-1-WB-anti-lrp5-antibody.jpgAnti-LRP5 Antibody Picoband&reg;Anti-LRP5 antibody&#44; PA2273&#44; All Western blotting<br>All lanes: Anti-LRP5(PA2273) at 0.5ug/ml <br>Lane 1: Rat Liver Tissue Lysate at 40ug <br>Lane 2: Mouse Liver Tissue Lysate at 40ug <br>Lane 3: NIH Whole Cell Lysate at 40ug <br>Predicted bind size: 179KD <br>Observed bind size: 179KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek2-antibody-pa2274-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2274-1-WB-anti-mek2-antibody.jpgAnti-MEK2/MAP2K2 Antibody Picoband&reg;Anti-MEK2 antibody&#44; PA2274&#44; All Western blotting<br>All lanes: MAP2K2(PA2274) at 0.5ug/ml <br>Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug <br>Lane 2: Rat Kidney Tissue Lysate at 40ug <br>Lane 3: HELA Whole Cell Lysate at 40ug <br>Lane 4: COLO320 Whole Cell Lysate at 40ug <br>Lane 5: PC12 Whole Cell Lysate at 40ug <br>Lane 6: JURKAT Whole Cell Lysate at 40ug <br>Lane 7: HT1080 Whole Cell Lysate at 40ug <br>Predicted bind size: 50KD <br>Observed bind size: 50KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek4-antibody-pa2275-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2275-map2k4-primary-antibodies-wb-testing-1.jpgAnti-MEK4/MAP2K4 Antibody Picoband&reg;Western blot analysis of MEK4/MAP2K4 using anti-MEK4/MAP2K4 antibody (PA2275). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK4/MAP2K4 antigen affinity purified polyclonal antibody (PA2275) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MEK4/MAP2K4 at approximately 44 kDa. The expected band size for MEK4/MAP2K4 is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mertk-antibody-pa2276-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2276-mertk-primary-antibodies-wb-testing-1.jpgAnti-Tyrosine-protein kinase Mer MERTK Antibody Picoband&reg; Western blot analysis of MERTK using anti-MERTK antibody (PA2276). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: monkey kidney tissue lysates, <br> Lane 5: rat testis tissue lysates, <br> Lane 6: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MERTK antigen affinity purified polyclonal antibody (Catalog # PA2276) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MERTK at approximately 120 kDa. The expected band size for MERTK is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2276-mertk-primary-antibodies-fcm-testing-2.jpgAnti-Tyrosine-protein kinase Mer MERTK Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-MERTK antibody (PA2276). <br>Overlay histogram showing HepG2 cells stained with PA2276 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MERTK Antibody (PA2276, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-plakophilin-2-antibody-pa2278-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2278-1-WB-anti-plakophilin-2-antibody.jpgAnti-Plakophilin 2/PKP2 Antibody Picoband&reg;Anti-Plakophilin 2 antibody&#44; PA2278&#44; All Western blotting<br>All lanes: Anti-PKP2(PA2278) at 0.5ug/ml<br> Lane 1: Rat Cardiac Muscle Tissue Lysate at 40ug<br> Lane 2: Human Placenta Tissue Lysate at 40ug<br> Lane 3: Rat Brain Tissue Lysate at 40ug<br> Lane 4: Rat Intestine Tissue Lysate at 40ug<br> Lane 5: HELA Whole Cell Lysate at 40ug<br> Lane 6: JURKAT Whole Cell Lysate at 40ug<br> Lane 7: 293T Whole Cell Lysate at 40ug<br> Lane 8: MCF-7 Whole Cell Lysate at 40ug<br> Lane 9: U87 Whole Cell Lysate at 40ug<br> Predicted bind size: 97KD<br> Observed bind size: 97KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab3c-antibody-pa2279-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2279-1-WB-anti-rab3c-antibody.jpgAnti-Ras-related protein Rab-3C Rab3C Antibody Picoband&reg;Anti-Rab3C antibody&#44; PA2279&#44; All Western blotting<br>All lanes: Anti-RAB3C(PA2279) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: 293T Whole Cell Lysate at 40ug<br> Lane 4: COLO320 Whole Cell Lysate at 40ug<br> Predicted bind size: 26KD<br> Observed bind size: 26KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab8a-antibody-pa2280-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2280-1-IHC-anti-rab8a-antibody.jpgAnti-Ras-related protein Rab-8A RAB8A Antibody Picoband&reg;Anti-RAB8A antibody&#44; PA2280&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2280-2-IHC-anti-rab8a-antibody.jpgAnti-Ras-related protein Rab-8A RAB8A Antibody Picoband&reg;Anti-RAB8A antibody&#44; PA2280&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2280-3_1.jpgAnti-Ras-related protein Rab-8A RAB8A Antibody Picoband&reg; Western blot analysis of RAB8A using anti-RAB8A antibody (PA2280). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> lane 1: Caco-2 whole cell lysates,<br> lane 2: HepG2 whole cell lysates, <br> lane 3: A549 whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB8A antigen affinity purified polyclonal antibody (Catalog # PA2280) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB8A at approximately 24KD. The expected band size for RAB8A is at 24KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab9-antibody-pa2281-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2281-1-WB-anti-rab9-antibody.jpgAnti-Rab9/RAB9A Antibody Picoband&reg;Anti-Rab9 antibody&#44; PA2281&#44; All Western blotting<br>All lanes: Anti-RAB9A(PA2281) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 40ug<br> Lane 2: Mouse Brain Tissue Lysate at 40ug<br> Lane 3: HELA Whole Cell Lysate at 40ug<br> Lane 4: PC12 Whole Cell Lysate at 40ug<br> Lane 5: NIH Whole Cell Lysate at 40ug<br> Lane 6: A431 Whole Cell Lysate at 40ug<br> Lane 7: 293T Whole Cell Lysate at 40ug<br> Predicted bind size: 23KD<br> Observed bind size: 23KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab11a-antibody-pa2282-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2282-1-WB-anti-rab11a-antibody.jpgAnti-Ras-related protein Rab-11A Rab11A Antibody Picoband&reg;Anti-Rab11A antibody&#44; PA2282&#44; All Western blotting<br>All lanes: Anti-RAB11A(PA2282) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: NIH Whole Cell Lysate at 40ug<br> Lane 3: A431 Whole Cell Lysate at 40ug<br> Predicted bind size: 24KD<br> Observed bind size: 24KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2282-rab11a-primary-antibodies-wb-testing-1.jpgAnti-Ras-related protein Rab-11A Rab11A Antibody Picoband&reg;Immunoprecipitating (IP) Rab11A in Hela whole cell lysate.<br> Western blot analysis of Rab11A using anti-Rab11A antibody (PA2282); <br> Lane 1: Hela whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Rab11A antibody in Hela whole cell lysate;<br> Lane 3: anti-Rab11A antibody (2μg) + Hela whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (PA2282) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sox2-antibody-pa2284-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-12935_2024_3371_fig1_html.pngAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg;Alantolactone suppressed STAT3 feedback activation induced by BRAFi, downregulating protein expression of Oct4 and Sox2 in A375 cells. ( A ) A375 cells were transfected with shSTAT3 plasmid, and the expression of STAT3 protein was detected 48 h later. ( B ) A375 cells were transfected with shSTAT3 plasmid for 24 h and treated with different concentrations of vemurafenib. After another 72 h, cell viability was detected by CCK8 assays. ( C ) Chemical structure of alantolactone. ( D ) Predicted model of alantolactone binding to STAT3β SH2, as shown by computational modeling. Protein structure information was obtained from Protein Data Bank (PDB) entry 6NJS. ( E ) Binding model of alantolactone to the SH2 domain. The molecular surface of the STAT3β SH2 domain is electrostatically colored with blue and red representing potentially positively- and negatively-charged regions, respectively. ( F ) Predicted interactions between the amino acid residues of the SH2 domain and alantolactone. Oxygen atoms of alantolactone are shown in red. Alantolactone forms carbon hydrogen bond with MET554, and there is a alkyl bond between alantolactone and ALA555. (G and H) A375 cells were treated with alantolactone and BRAFi single or combination for 6 ( G ) or 24 ( H ) hours, phospho-STAT3 (705), STAT3, phospho-ERK1/2, ERK1/2, SOX2, Oct4, c-Myc, Klf4, cyclin D1 and cleaved PARP levels were analyzed by western blotting, and tubulin served as a loading control. (I and J) A375 cells were treated with different concentrations of alantolactone and vemurafenib ( I ) or alantolactone and dabrafenib ( J ) for 3 days. Cell viability was determined by CCK8 assays. The two images on the left in Figures G and H are split from the image on the right. Data are mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t -test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-024-03371-9'>38822350</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-12935_2024_3371_fig2_html.pngAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg;The combination of alantolactone and MAPKi simultaneously inhibited the STAT3 and BRAF/MEK/ERK pathways, regulating the expression level of downstream effectors in A375 cells. ( A ) A375 cells were treated with vemurafenib, cobimetinib and alantolactone alone or in combination for 6 h. The gene expression levels of c-Myc, Klf4, Sox2 and Oct4 were detected by real-time fluorescent quantitative PCR, and β-actin served as a loading control. ( B ) A375 cells were treated with vemurafenib, cobimetinib and alantolactone for 24 h. Phospho-STAT3 (705), STAT3, phospho-ERK1/2, ERK1/2, Sox2, Oct4, c-Myc, Klf4, cyclin D1 and cleaved PARP levels were analyzed by western blotting, and tubulin served as a loading control. (C and D) A375 cells were treated with different concentrations of cobimetinib and alantolactone ( C ) or trametinib and alantolactone ( D ) for 72 h. Cell viability was determined by CCK8 assays. The two images on the left in Figures C and D are split from the image on the right. (E and F) A375 cells were treated with different concentrations of vemurafenib, cobimetinib, and alantolactone ( E ) or dabrafenib, trametinib and alantolactone ( F ) for 72 h. Cell viability was determined by CCK8 assays. The two images on the left in Figures C and D are split from the image on the right. ( G ) A375 cells were treated with vemurafenib (5 µM), cobimetinib (1 µM), and alantolactone (6 µM) alone or in combination for 24 h. Flow cytometry analysis of cell death (Annexin V/PI labelling) in A375 cells. The histogram on the right shows the proportion of dead cells. Data, means ± SDs. * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-024-03371-9'>38822350</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-12935_2024_3371_fig3_html.pngAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg;Combined treatment with alantolactone and MAPK pathway inhibitors showed highly selective cytotoxic effects on A375 cells, but had no obvious cytotoxicity on renal tubular epithelial cells. ( A ) HK2 and A375 cells were treated with vemurafenib and alantolactone or vemurafenib, cobimetinib and alantolactone for 24 h. Phospho-STAT3 (705), STAT3, phospho-ERK1/2, ERK1/2, Sox2, c-Myc and cleaved PARP levels were analyzed by western blotting, and tubulin served as a loading control. ( B ) HK2 and A375 cells were treated with different concentrations of alantolactone, vemurafenib and cobimetinib for 72 h. Cell viability was determined by CCK8 assays. Data are the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-024-03371-9'>38822350</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-12935_2024_3371_fig4_html.pngAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg;Alantolactone could synergistically enhance the cytotoxic effects with MAPKi in A375 xenografts of nude mice. ( A ) A375 xenografts were administrated by alantolactone (ATL; 20 mg/kg administered intraperitoneally once daily) and vemurafenib (Vem; 25 mg/kg administered intraperitoneally once daily) + cobimetinib (Cobi; 1 mg/kg administered intraperitoneally once daily) individually or in combination. During the treatment period, measure and record the tumor volume every other day. ( B ) After 12 days of treatment, tumor grafts were removed and weighed. ( C ) Photographs of xenograft A375 tumors treated with single or combination drugs. ( D ) During the drug treatment, the animals were weighed every other day. ( E ) Haematoxylin-eosin staining of heart, liver and kidney tissue sections (magnification: ×100). ( F ) Representative images of immunohistochemical staining of p-STAT3(705), p-ERK1/2, c-Myc, Klf4, Sox2, Oct4 and Ki67 in tumor tissues. Data are the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-024-03371-9'>38822350</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-12935_2024_3371_fig5_html.pngAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg;Alantolactone sensitized intrinsic-resistant A2058 cells to MAPKi targeted therapy by inhibiting STAT3 activation. ( A ) A2058 cells were treated with vemurafenib and alantolactone or vemurafenib, cobimetinib and alantolactone for 24 h. Phospho-STAT3 (705), STAT3, phospho-ERK1/2, ERK1/2, Sox2, Oct4, c-Myc, Klf4, cyclin D1 and cleaved PARP levels were analyzed by western blotting, and tubulin served as a loading control. (B-D) A2058 cells were treated with different concentrations of vemurafenib and alantolactone ( B ), cobimetinib and alantolactone ( C ), or vemurafenib, cobimetinib and alantolactone ( D ) for 72 h. Cell viability was determined by CCK8 assays. Data are the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-024-03371-9'>38822350</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-12935_2024_3371_fig6_html.pngAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg;The combination of alantolactone and MAPKi could inhibit the proliferation of MAPKi-resistant A375R cells. ( A ) A375R cells were treated with vemurafenib and alantolactone or vemurafenib, cobimetinib and alantolactone for 24 h. Phospho-STAT3 (705), STAT3, phospho-ERK1/2, ERK1/2, Sox2, Oct4, c-Myc, Klf4, cyclin D1 and cleaved PARP levels were analyzed by western blotting, and tubulin served as a loading control. (B-D) A375R cells were treated with different concentrations of vemurafenib and alantolactone ( B ), cobimetinib and alantolactone ( C ), or vemurafenib, cobimetinib and alantolactone ( D ) for 72 h. Cell viability was determined by CCK8 assays. Data are the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-024-03371-9'>38822350</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-12935_2024_3371_fig7_html.pngAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg;Alantolactone could synergistically enhance the cytotoxic effects with MAPKi in A375 xenografts of nude mice. ( A ) Tumor growth curves of A375R xenograft models treated with alantolactone (ATL; 20 mg/kg administered intraperitoneally once daily) and vemurafenib (Vem; 25 mg/kg administered intraperitoneally once daily) + cobimetinib (Cobi; 1 mg/kg administered intraperitoneally once daily) alone or in combination. ( B ) After 12 days of treatment, tumor grafts were removed and weighed. ( C ) Photographs of xenograft A375R tumors treated with single or combination drugs. ( D ) During the drug treatment, the animals were weighed every other day. ( E ) Representative images of immunohistochemical staining of p-STAT3(705), p-ERK1/2, c-Myc, Klf4, Sox2, Oct4 and Ki67 in tumor tissues. Data are the mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; Student’s t test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-024-03371-9'>38822350</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-sox2-primary-antibodies-wb-testing-1.jpgAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg; Western blot analysis of SOX2 using anti-SOX2 antibody (PA2284). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates, <br> Lane 2: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 antigen affinity purified polyclonal antibody (Catalog # PA2284) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-sox2-primary-antibodies-ihc-testing-2.jpgAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA2284). <br> SOX2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA2284) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-sox2-primary-antibodies-ihc-testing-3.jpgAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA2284). <br> SOX2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA2284) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2284-sox2-primary-antibodies-ihc-testing-4.jpgAnti-Transcription factor SOX-2 SOX2 Antibody Picoband&reg; IHC analysis of SOX2 using anti-SOX2 antibody (PA2284). <br> SOX2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA2284) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sox7-antibody-pa2285-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2285-1-WB-anti-sox7-antibody.jpgAnti-SOX7 Antibody Picoband&reg;Anti-SOX7 antibody&#44; PA2285&#44; All Western blotting<br>All lanes: Anti-SOX7(PA2285) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 40ug<br> Lane 2: Human Placenta Tissue Lysate at 40ug<br> Lane 3: Rat Lung Tissue Lysate at 40ug<br> Lane 4: Rat Testis Tissue Lysate at 40ug<br> Lane 5: HELA Whole Cell Lysate at 40ug<br> Lane 6: A549 Whole Cell Lysate at 40ug<br> Lane 7: HEPG2 Whole Cell Lysate at 40ug<br> Lane 8: SMMC Whole Cell Lysate at 40ug<br> Lane 9: NEURO Whole Cell Lysate at 40ug<br> Predicted bind size: 42KD<br> Observed bind size: 42KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-st5-antibody-pa2287-boster.html2026-03-24T05:04:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2287-1-WB-anti-st5-antibody.jpgAnti-ST5 Antibody Picoband&reg;Anti-ST5 antibody&#44; PA2287&#44; All Western blotting<br>All lanes: Anti-ST5(PA2287) at0.5ug/ml<br>Lane 1: Rat Testis Tissue Lysate at 40ug<br>Lane 2: A431 Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Lane 4: COLO320 Whole Cell Lysate at 40ug<br>Lane 5: NIH/3T3 Whole Cell Lysate at 40ug<br>Predicted bind size: 126KD<br>Observed bind size: 126KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tmem16a-antibody-pa2290-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2290-1-WB-anti-tmem16a-antibody.jpgAnti-TMEM16A/ANO1 Antibody Picoband&reg;Anti-TMEM16A antibody&#44; PA2290&#44; All Western blotting<br>All lanes: Anti-ANO1(PA2290) at 0.5ug/ml<br> Lane 1: Rat Liver Tissue Lysate at 40ug<br> Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug<br> Predicted bind size: 114KD<br> Observed bind size: 114KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cav1-3-antibody-pa2293-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2293-1-WB-anti-cav1-3-antibody.jpgAnti-CaV1.3/CACNA1D Antibody Picoband&reg;Anti-CaV1.3 antibody&#44; PA2293&#44; All Western blotting<br>All lanes: Anti-CACNA1D(PA2293) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 40ug<br> Predicted bind size: 245KD<br> Observed bind size: 245KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-syncam-antibody-pa2294-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2294-1-WB-anti-syncam-antibody.jpgAnti-SynCAM/CADM1 Antibody Picoband&reg;Anti-SynCAM antibody&#44; PA2294&#44; All Western blotting<br>All lanes: Anti-CADM1(PA2294) at 0.5ug/ml<br> Lane 1: A549 Whole Cell Lysate at 40ug<br> Lane 2: JURKAT Whole Cell Lysate at 40ug<br> Lane 3: RAJI Whole Cell Lysate at 40ug<br> Lane 4: HELA Whole Cell Lysate at 40ug<br> Predicted bind size: 60KD<br> Observed bind size: 60KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psd95-antibody-pa2295-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2295-dlg4-primary-antibodies-ihc-testing-4.jpgAnti-PSD95/DLG4 Antibody Picoband&reg;IHC analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (PA2295). <br>PSD95/DLG4 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSD95/DLG4 Antibody (PA2295) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2295-dlg4-primary-antibodies-wb-testing-1.jpgAnti-PSD95/DLG4 Antibody Picoband&reg; Western blot analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (PA2295). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates,<br> Lane 3: human U-87MG whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD95/DLG4 antigen affinity purified polyclonal antibody (Catalog # PA2295) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD95/DLG4 at approximately 90-100 kDa. The expected band size for PSD95/DLG4 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2295-dlg4-primary-antibodies-ihc-testing-2.jpgAnti-PSD95/DLG4 Antibody Picoband&reg; IHC analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (PA2295). <br> PSD95/DLG4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSD95/DLG4 Antibody (PA2295) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2295-dlg4-primary-antibodies-ihc-testing-3.jpgAnti-PSD95/DLG4 Antibody Picoband&reg; IHC analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (PA2295). <br> PSD95/DLG4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSD95/DLG4 Antibody (PA2295) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2295-dlg4-primary-antibodies-if-testing-4.jpgAnti-PSD95/DLG4 Antibody Picoband&reg; IF analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (PA2295). <br> PSD95/DLG4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSD95/DLG4 Antibody (PA2295) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kv1-1-potassium-channel-antibody-pa2296-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2296-1-WB-anti-kv1-1-potassium-channel-antibody.jpgAnti-Kv1.1 potassium channel/KCNA1 Antibody Picoband&reg;Anti-Kv1.1 potassium channel antibody&#44; PA2296&#44; All Western blotting<br>All lanes: Anti-KCNA1(PA2296) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 40ug<br> Lane 2: Rat Testis Tissue Lysate at 40ug<br> Lane 3: Rat Cardiac Muscle Tissue Lysate at 40ug<br> Lane 4: HELA Whole Cell Lysate at 40ug<br> Lane 5: U87 Whole Cell Lysate at 40ug<br> Lane 6: SHG Whole Cell Lysate at 40ug<br> Lane 7: NEURO Whole Cell Lysate at 40ug<br> Predicted bind size: 56KD<br> Observed bind size: 56KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kv1-4-antibody-pa2297-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2297-1-WB-anti-kv1-4-antibody.jpgAnti-Kv1.4/KCNA4 Antibody Picoband&reg;Anti-Kv1.4 antibody&#44; PA2297&#44; All Western blotting<br>All lanes: Anti-KCNA4(PA2297) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 40ug<br> Lane 2: HY1080Whole Cell Lysate at 40ug<br> Lane 3: PANC Whole Cell Lysate at 40ug<br> Lane 4: U87 Whole Cell Lysate at 40ug<br> Lane 5: SHG Whole Cell Lysate at 40ug<br> Predicted bind size: 70KD<br> Observed bind size: 70KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-muc1-antibody-pa2302-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2302-1-WB-anti-muc1-antibody.jpgAnti-Mucin-1 MUC1 Antibody Picoband&reg;Anti-MUC1 antibody&#44; PA2302&#44; All Western blotting<br>All lanes: Anti-MUCIN1(PA2302) at 0.5ug/ml<br> Lane 1: COLO320 Whole Cell Lysate at 40ug<br> Lane 2: SW620 Whole Cell Lysate at 40ug<br> Lane 3: HEPG2 Whole Cell Lysate at 40ug<br> Lane 4: MCF-7 Whole Cell Lysate at 40ug<br> Lane 5: JURKAT Whole Cell Lysate at 40ug<br> Predicted bind size: 122KD<br> Observed bind size: 170KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mucin-5ac-antibody-pa2303-boster.html2026-03-24T05:04:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2303-1-WB-anti-mucin-5ac-antibody.jpgAnti-Mucin 5AC/MUC5AC Antibody Picoband&reg;Anti-Mucin 5AC antibody&#44; PA2303&#44; All Western blotting<br>All lanes: Anti-MUC5AC(PA2303) at0.5ug/ml<br>Lane 1: SGC Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: COLO320 Whole Cell Lysate at 40ug<br>Lane 4: SW620 Whole Cell Lysate at 40ug<br>Lane 5: MCF-7 Whole Cell Lysate at 40ug<br>Lane 6: PANC Whole Cell Lysate at 40ug<br>Predicted bind size: 527KD<br>Observed bind size: 300KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa2303-2.jpgAnti-Mucin 5AC/MUC5AC Antibody Picoband&reg; IHC analysis of Mucin 5AC using anti-Mucin 5AC antibody (PA2303). <br> Mucin 5AC was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Mucin 5AC Antibody (PA2303) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpc4-antibody-pa2307-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2307-1-WB-anti-trpc4-antibody.jpgAnti-TRPC4 Antibody Picoband&reg;Anti-TRPC4 antibody&#44; PA2307&#44; All Western blotting<br>All lanes: Anti-TRPC4(PA2307) at 0.5ug/ml<br> Lane 1: COLO320 Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Lane 3: PANC Whole Cell Lysate at 40ug<br> Predicted bind size: 112KD<br> Observed bind size: 112KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gad67-antibody-pa1036-1-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1036-1-gad67-primary-antibodies-wb-testing-1.jpgAnti-GAD67/GAD1 Antibody Picoband&reg;Western blot analysis of GAD67 using anti-GAD67 antibody (PA1036-1). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD67 antigen affinity purified polyclonal antibody (PA1036-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GAD67 at approximately 67 kDa. The expected band size for GAD67 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1036-1-gad67-primary-antibodies-ihc-testing-1.jpgAnti-GAD67/GAD1 Antibody Picoband&reg;IHC analysis of GAD67 using anti-GAD67 antibody (PA1036-1). <br>GAD67 was detected in a paraffin-embedded section of human celebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GAD67 Antibody (PA1036-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1036-1-gad67-primary-antibodies-if-testing-1.jpgAnti-GAD67/GAD1 Antibody Picoband&reg;IF analysis of GAD67 using anti-GAD67 antibody (PA1036-1). <br> GAD67 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GAD67 Antibody (PA1036-1) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-d1-antibody-pa1245-2-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1245-2-1-WB-anti-cyclin-d1-antibody.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg; Western blot analysis of Cyclin D1 using anti-Cyclin D1 antibody (PA1245-2). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Testis Tissue Lysate&#44;<br> Lane 2: Human Placenta Tissue Lysate&#44;<br> Lane 3: Rat Brain Tissue Lysate&#44;<br> Lane 4: MCF-7 Whole Cell Lysate&#44;<br> Lane 5: COLO320 Whole Cell Lysate&#44;<br> Lane 6: SW620 Whole Cell Lysate&#44;<br> Lane 7: MM231 Whole Cell Lysate.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D1 antigen affinity purified polyclonal antibody (Catalog # PA1245-2) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D1 at approximately 33KD. The expected band size for Cyclin D1 is at 33KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1245-2-ccnd1-primary-antibodies-fcm-testing-2.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-Cyclin D1 antibody (PA1245-2). <br> Overlay histogram showing A431 cells stained with PA1245-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin D1 Antibody (PA1245-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-beta-antibody-pa1361-1-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1361-1-1-WB-anti-tnf-beta-antibody.jpgAnti-TNF beta/LTA Antibody Picoband&reg;Anti-TNF beta antibody&#44; PA1361-1&#44; All Western blotting<br>All lanes: Anti-TNF beta(PA1361-1) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Lane 3: COLO320 Whole Cell Lysate at 40ug<br> Predicted bind size: 22KD<br> Observed bind size: 22KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grb7-antibody-pa1589-1-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1589-1-grb7-primary-antibodies-wb-testing-1.jpgAnti-GRB7 Antibody Picoband&reg;Western blot analysis of GRB7 using anti-GRB7 antibody (PA1589-1). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB7 antigen affinity purified polyclonal antibody (PA1589-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRB7 at approximately 60 kDa. The expected band size for GRB7 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1589-1-grb7-primary-antibodies-ihc-testing-1.jpgAnti-GRB7 Antibody Picoband&reg;IHC analysis of GRB7 using anti-GRB7 antibody (PA1589-1). <br>GRB7 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRB7 Antibody (PA1589-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1589-1-grb7-primary-antibodies-ihc-testing-2.jpgAnti-GRB7 Antibody Picoband&reg;IHC analysis of GRB7 using anti-GRB7 antibody (PA1589-1). <br>GRB7 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRB7 Antibody (PA1589-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1589-1-grb7-primary-antibodies-fcm-testing-1.jpgAnti-GRB7 Antibody Picoband&reg;Flow Cytometry analysis of CACO-2 cells using anti-GRB7 antibody (PA1589-1). <br> Overlay histogram showing CACO-2 cells stained with PA1589-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRB7 Antibody (PA1589-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c5-c5a-antibody-pa2308-boster.html2026-03-25T05:21:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2308-1-IHC-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg;Anti-C5/C5a antibody&#44; PA2308&#44; IHC(P)<br>IHC(P): Mouse Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2308-2-IHC-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg;Anti-C5/C5a antibody&#44; PA2308&#44; IHC(P)<br>IHC(P): Rat Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2308-3-IHC-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg;Anti-C5/C5a antibody&#44; PA2308&#44; IHC(P)<br>IHC(P): Rat Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2308-4-WB-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg;Anti-C5/C5a antibody&#44; PA2308&#44; All Western blotting<br>All lanes: Anti-C5a(PA2308) at 0.5ug/ml<br> Lane 1: Rat Liver Tissue Lysate at 40ug<br> Lane 2: Mouse Liver Tissue Lysate at 40ug<br> Lane 3: NIH Whole Cell Lysate at 40ug<br> Lane 4: HEPA Whole Cell Lysate at 40ug<br> Lane 5: PC12 Whole Cell Lysate at 40ug<br> Predicted bind size: 115KD<br> Observed bind size: 115KD https://www.bosterbio.com/products/primary-antibodies/anti-c5-c5a-antibody-pa2308-1-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa2308-1-1-WB-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg;Anti-C5/C5a antibody&#44; PA2308-1&#44; All Western blotting<br>All lanes: Anti-C5a(PA2308-1) at 0.5ug/ml<br> WB: Rat Liver Tissue Lysate at 40ug<br> Predicted bind size: 115KD<br> Observed bind size: 115KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adiponectin-picoband-trade-antibody-pb9001-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9001-1-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001). <br> Adiponectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9001-2-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001). <br> Adiponectin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9001-3-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9001). <br> Adiponectin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Adiponectin Antibody (PB9001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9001-4_1-WB-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; Western blot analysis of Adiponectin using anti-Adiponectin antibody (PB9001). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adiponectin antigen affinity purified polyclonal antibody (Catalog # PB9001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adiponectin at approximately 30 kDa. The expected band size for Adiponectin is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cox1-picoband-trade-antibody-pb9002-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9002-ptgs1-primary-antibodies-wb-testing-1.jpgAnti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband&reg; Western blot analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: rat L6 whole cell lysates, <br> Lane 4: mouse C2C12 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 antigen affinity purified polyclonal antibody (Catalog # PB9002) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX1/Cyclooxygenase 1/PTGS1 at approximately 65-72 kDa. The expected band size for COX1/Cyclooxygenase 1/PTGS1 is at 69 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9002-ptgs1-primary-antibodies-ihc-testing-2.jpgAnti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband&reg; IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). <br> COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9002-ptgs1-primary-antibodies-fcm-testing-7.pngAnti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). <br>Overlay histogram showing HEL cells stained with PB9002 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9002-ptgs1-primary-antibodies-ihc-testing-3.jpgAnti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband&reg; IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). <br> COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9002-ptgs1-primary-antibodies-ihc-testing-4.jpgAnti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband&reg; IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). <br> COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9002-ptgs1-primary-antibodies-ihc-testing-5.jpgAnti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband&reg; IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). <br> COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9002-ptgs1-primary-antibodies-ihc-testing-6.jpgAnti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband&reg; IHC analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). <br> COX1/Cyclooxygenase 1/PTGS1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COX1/Cyclooxygenase 1/PTGS1 Antibody (PB9002) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gm-csf-picoband-trade-antibody-pb9003-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9003-1-IHC-anti-gm-csf-picoband-antibody.jpgAnti-GM-CSF/CSF2 Antibody Picoband&reg; IHC analysis of GM-CSF using anti-GM-CSF antibody (PB9003). <br> GM-CSF was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GM-CSF Antibody (PB9003) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9003-2-WB-anti-gm-csf-picoband-antibody.jpgAnti-GM-CSF/CSF2 Antibody Picoband&reg; Western blot analysis of GM-CSF using anti-GM-CSF antibody (PB9003). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant mouse GM-CSF protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GM-CSF antigen affinity purified polyclonal antibody (Catalog # PB9003) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GM-CSF at approximately 17 kDa. The expected band size for GM-CSF is at 17 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-6-picoband-trade-antibody-pb9005-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9005-1-WB-anti-il-6-antibody.jpgAnti-IL6 Antibody Picoband&reg; Western blot analysis of IL6 using anti-IL6 antibody (PB9005). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant rat IL6 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # PB9005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 25 kDa. The expected band size for IL6 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9005-12276_2021_702_fig4_html.pngAnti-IL6 Antibody Picoband&reg;Inflammation response were reduced by NLRC4 siRNA after ICH. a – g , j Western blot assay to detect NLRC4, pNLRC4, Pro-IL-1β, IL-1β, Pro-caspase-1, caspase-1, Pro-IL-18, IL-18, TNF-α, and IL-6, ( k , l ) ELISA for IL–18 and IL–1β ( h , i ) Immunostaining for MPO-positive cells (×400 and ×200) at peri-hematoma area in sham, ICH, negative control siRNA, and NLRC4 siRNA groups at 72 h after ICH (six rats for each group). * P < 0.05, compared with ICH. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs12276-021-00702-y'>34848837</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9005-12974_2020_1764_fig2_html.pngAnti-IL6 Antibody Picoband&reg;DJ-1 interference increased the expression of TNF-α, IL-1β, and IL-6 after cerebral I/R injury. a and c Western blot detecting DJ-1 and the cytokines TNF-α, IL-1β, and IL-6 in rats. b , d Western blot detecting DJ-1 and the cytokines TNF-α, IL-1β, and IL-6 in astrocytes. e–g Quantification of TNF-α, IL-1β, and IL-6 in rats by ELISA. h‑j Quantification of TNF-α, IL-1β, and IL-6 in astrocytes by ELISA. The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 6 per group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9005-12974_2020_1764_fig5_html.pngAnti-IL6 Antibody Picoband&reg;DJ-1 inhibited the expression of TNF-α, IL-1β, and IL-6 after cerebral I/R injury via SHP-1. a , c After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. b , d After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e , g After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. f , h After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. i , k , m Quantification of TNF-α, IL-1β, and IL-6 in rats by ELISA. j , l , n Quantification of TNF-α, IL-1β, and IL-6 in astrocytes by ELISA. The data are expressed as the mean ± SEM. * p < 0.05 vs. the sham group; # p < 0.05 vs. the MCAO group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group; ◆ p < 0.05 vs. the TPI-1 group. n = 6 per group. The data are expressed as the mean ± SEM. * p < 0.05 vs. the control group; # p < 0.05 vs. the OGD/R group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group; ◆ p < 0.05 vs. the TPI-1 group. n = 6 per group. The groups in a and b and their corresponding groups in the statistical graphs are as follows: sham (−−−), MCAO or OGD/R (+−−), scramble (+−−), overexpression (++−), overexpression + DMSO (++−), and overexpression + TPI-1 (+++). The groups in e and f and the corresponding groups in the statistical graphs are as follows: DMSO (+−−), TPI-1 (+−+), overexpression + DMSO (++−), overexpression + TPI-1 (+++) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9005-12974_2020_1764_fig6_html.pngAnti-IL6 Antibody Picoband&reg;DJ-1 regulated the disassociation of NLRX1 from TRAF6 after cerebral I/R injury via SHP-1. a , g After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in rats. b , h After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in astrocytes. c , i After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. d , j After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e Immunoprecipitation and immunoblot analyses of NLRX1-TRAF6 in rats. f Immunoprecipitation and immunoblot analyses of SHP-1-TRAF6 in rats. The data are expressed as the mean ± SEM. * p < 0.05 vs. the sham group; # p < 0.05 vs. the MCAO group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group. n = 6 per group. The data are expressed as the mean ± SEM. * p < 0.05 vs. the control group; # p < 0.05 vs. the OGD/R group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group. n = 6 per group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9005-fphar-09-00497-g003.jpgAnti-IL6 Antibody Picoband&reg;Effects of acacetin on inflammation-related cytokines in cells with hypoxia/reoxygenation exposure. Western blots and mean relative level of IL-6 (A) , TLR-4 (B) , IL-10 (C) in neonatal rat cardiomyocytes without (control) or with hypoxia/reoxygenation (H/R) exposure in the absence (V, vehicle) or presence of 0.3, 1, or 3 μM acacetin. Western blots and mean relative level of IL-6 (D) , TLR-4 (E) , IL-10 (F) in H9C2 cardiomyoblasts with the treatment used in (A–C) . Data were expressed as mean ± SEM and analyzed by one-way ANOVA followed by the Bonferroni-test ( n = 5 individual experiments, ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. hypoxia/reoxygenation alone).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00497/full'>29867499</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9005-fphar-09-00497-g008.jpgAnti-IL6 Antibody Picoband&reg;Effects of silencing Nrf2 on apoptosis- and inflammation-related proteins in cells with hypoxia/reoxygenation insult. Western blots and relative levels of Bcl-2 (A) , Bax (B) , and cleaved caspase-1 (C) in H9C2 cardiomyoblasts transfected with control siRNA or Nrf2 siRNA and subjected to hypoxia/reoxygenation insult in the absence (V, vehicle) or presence of 3 μM acacetin (Aca). Western blots and relative levels of IL-6 (D) , TRL-4 (E) , and IL-10 (F) in H9C2 cardiomyoblasts with the treatment used in (A–C) . Data were expressed as mean ± SEM and analyzed by one-way ANOVA followed by Bonferroni-test ( n = 5 individual experiments, ∗ P < 0.05, ∗∗ P < 0.01 vs. vehicle of control siRNA; ## P < 0.01 vs. control siRNA with acacetin).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00497/full'>29867499</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-18-picoband-trade-antibody-pb9006-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9006-il18-primary-antibodies-wb-testing-1.jpgAnti-IL18 Antibody Picoband&reg; Western blot analysis of IL18 using anti-IL18 antibody (PB9006). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9006) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9006-il18-primary-antibodies-ihc-testing-2.jpgAnti-IL18 Antibody Picoband&reg; IHC analysis of IL18 using anti-IL18 antibody (PB9006). <br> IL18 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (PB9006) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9006-il18-primary-antibodies-if-testing-3.jpgAnti-IL18 Antibody Picoband&reg; IF analysis of IL18 using anti-IL18 antibody (PB9006) and anti-Beta Tubulin antibody (M01857-3).<br> IL18 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IL18 Antibody (PB9006) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-beta-4-picoband-trade-antibody-pb9007-boster.html2026-03-24T05:04:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9007-itgb4-primary-antibodies-wb-testing-1.jpgAnti-Integrin beta 4/ITGB4 Antibody Picoband&reg; Western blot analysis of ITGB4 using anti-ITGB4 antibody (PB9007). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human PC-3 whole cell lysates,<br> Lane 3: human T-47D whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: human Caco-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB4 antigen affinity purified polyclonal antibody (Catalog # PB9007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGB4 at approximately 210 kDa. The expected band size for ITGB4 is at 202 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9007-2-IHC-anti-integrin-beta-4-picoband-antibody.jpgAnti-Integrin beta 4/ITGB4 Antibody Picoband&reg; IHC analysis of ITGB4 using anti-ITGB4 antibody (PB9007).<br> ITGB4 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ITGB4 Antibody (PB9007) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9007-1-ihc-anti-integrin-beta-3-picoband-antibody.jpgAnti-Integrin beta 4/ITGB4 Antibody Picoband&reg; IHC analysis of ITGB4 using anti-ITGB4 antibody (PB9007).<br> ITGB4 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ITGB4 Antibody (PB9007) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9007-4.jpgAnti-Integrin beta 4/ITGB4 Antibody Picoband&reg; IF analysis of ITGB4 using anti-ITGB4 antibody (PB9007) <br> ITGB4 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ITGB4 Antibody (PB9007) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9007-5.pngAnti-Integrin beta 4/ITGB4 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ITGB4 antibody (PB9007). <br> Overlay histogram showing A431 cells stained with PB9007 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITGB4 Antibody (PB9007&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p53-picoband-trade-antibody-pb9008-boster.html2026-04-01T05:01:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9008-p53-primary-antibodies-wb-testing-1_1.jpgAnti-P53/TP53 Antibody Picoband&reg; Western blot analysis of P53 using anti-P53 antibody (PB9008). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human 293T whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P53 antigen affinity purified polyclonal antibody (Catalog # PB9008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P53 at approximately 53 kDa. The expected band size for P53 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9008-p53-primary-antibodies-wb-testing-1.jpgAnti-P53/TP53 Antibody Picoband&reg;Western blot analysis of P53 using anti-P53 antibody (PB9008).<br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br> Lane 1: huamn MDA-MB-231 whole cell lysates,<br> Lane 2: human MDA-MB-231 whole cell lysates,<br> Lane 3: human HCC1937 whole cell lysates,<br> Lane 4: human HCC1937 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P53 antigen affinity purified polyclonal antibody (PB9008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P53 at approximately 53 kDa. The expected band size for P53 is at 53 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9008-p53-primary-antibodies-ihc-testing-2_1.jpgAnti-P53/TP53 Antibody Picoband&reg; IHC analysis of P53 using anti-P53 antibody (PB9008). <br> P53 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P53 Antibody (PB9008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9008-p53-primary-antibodies-if-testing-3.jpgAnti-P53/TP53 Antibody Picoband&reg; IF analysis of P53 using anti-P53 antibody (PB9008). <br> P53 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- P53 Antibody (PB9008) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9008-p53-primary-antibodies-fcm-testing-4.jpgAnti-P53/TP53 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-P53 antibody (PB9008). <br> Overlay histogram showing A431 cells stained with PB9008 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-P53 Antibody (PB9008, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-progesterone-receptor-picoband-trade-antibody-pb9009-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9009-1-IHC-anti-progesterone-receptor-picoband-antibody.jpgAnti-Progesterone Receptor/PGR Antibody Picoband&reg; IHC analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009).<br> Progesterone Receptor was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9009-2_1.jpgAnti-Progesterone Receptor/PGR Antibody Picoband&reg; Western blot analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human T-47D whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Progesterone Receptor antigen affinity purified polyclonal antibody (Catalog # PB9009) at 0.1 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Progesterone Receptor at approximately 90 KD (PR-A)&#44; 118KD (PR-B). The expected band size for Progesterone Receptor is at 99KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9009-pgr-primary-antibody-if-testing-3.jpgAnti-Progesterone Receptor/PGR Antibody Picoband&reg; IF analysis of Progesterone Receptor using anti-Progesterone Receptor antibody (PB9009). <br> Progesterone Receptor was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Progesterone Receptor Antibody (PB9009) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-alpha-picoband-trade-antibody-pb9010-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9010-1-WB-anti-tnf-alpha-picoband-antibody.jpgAnti-TNF alpha Antibody Picoband&reg; Western blot analysis of TNF alpha using anti-TNF alpha antibody (PB9010).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: PC-12 Whole Cell Lysate,<br> Lane 2: Rat Spleen Tissue Lysate,<br> Lane 3: Rat Brain Tissue Lysate,<br> Lane 4: Rat Kidney Tissue Lysate,<br> Lane 5: Rat Liver Tissue Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF alpha antigen affinity purified polyclonal antibody (Catalog # PB9010) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF alpha at approximately 26KD. The expected band size for TNF alpha is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9010-12974_2021_2198_fig8_html.pngAnti-TNF alpha Antibody Picoband&reg;Red nucleus IL-33 facilitates the early development of mononeuropathic pain by inducing TNF-α through activating ERK, p38 MAPK and JAK2/STAT3 signaling pathways. A Western blotting showed that red nucleus TNF-α was increased at 1 week post-SNI, intrarubral injection of anti-IL-33 antibody at 1 week post-SNI suppressed the overexpression of TNF-α ( n = 6 per group, F = 14.302, P < 0.001). B Western blotting displayed that intrarubral administration of PD98059, SB203580, or AG490 at 1 week post-SNI inhibited the production of TNF-α ( n = 6 per group, F = 13.157, P < 0.001). C Immunohistochemistry showed that red nucleus TNF-α was upregulated at 1 week post-SNI, intrarubral injection of anti-IL-33 antibody, PD98059, SB203580, or AG490 at 1 week post-SNI suppressed the production of TNF-α ( n = 4 per group, F = 33.029, P < 0.001). ** P < 0.01 and *** P < 0.001. Scale bars = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-021-02198-9'>34225736</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9010-12974_2021_2198_fig9_html.pngAnti-TNF alpha Antibody Picoband&reg;Red nucleus IL-33 evokes mechanical hypersensitivity by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3 signaling pathways. A Western blotting indicated that intrarubral administration of IL-33 stimulated the secretion of TNF-α in naive rats ( n = 6 per group, F = 15.143, P < 0.001). B Western blotting showed that intrarubral pre-injection of PD98059, SB203580, or AG490, 30 min before IL-33 administration, restrained IL-33-induced overexpression of TNF-α in naive rats ( n = 6 per group, F = 9.812, P < 0.001). C Immunohistochemistry showed that intrarubral injection of IL-33 potentiated the secretion of TNF-α in naive rats, intrarubral pre-injection of PD98059, SB203580, or AG490, 30 min prior to IL-33 administration, inhibited IL-33-induced overexpression of TNF-α in naive rats ( n = 4 per group, F = 44.310, P < 0.001). *** P < 0.001. Scale bars = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-021-02198-9'>34225736</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9010-12974_2020_1764_fig2_html.pngAnti-TNF alpha Antibody Picoband&reg;DJ-1 interference increased the expression of TNF-α, IL-1β, and IL-6 after cerebral I/R injury. a and c Western blot detecting DJ-1 and the cytokines TNF-α, IL-1β, and IL-6 in rats. b , d Western blot detecting DJ-1 and the cytokines TNF-α, IL-1β, and IL-6 in astrocytes. e–g Quantification of TNF-α, IL-1β, and IL-6 in rats by ELISA. h‑j Quantification of TNF-α, IL-1β, and IL-6 in astrocytes by ELISA. The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 6 per group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9010-12974_2020_1764_fig5_html.pngAnti-TNF alpha Antibody Picoband&reg;DJ-1 inhibited the expression of TNF-α, IL-1β, and IL-6 after cerebral I/R injury via SHP-1. a , c After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. b , d After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e , g After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. f , h After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. i , k , m Quantification of TNF-α, IL-1β, and IL-6 in rats by ELISA. j , l , n Quantification of TNF-α, IL-1β, and IL-6 in astrocytes by ELISA. The data are expressed as the mean ± SEM. * p < 0.05 vs. the sham group; # p < 0.05 vs. the MCAO group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group; ◆ p < 0.05 vs. the TPI-1 group. n = 6 per group. The data are expressed as the mean ± SEM. * p < 0.05 vs. the control group; # p < 0.05 vs. the OGD/R group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group; ◆ p < 0.05 vs. the TPI-1 group. n = 6 per group. The groups in a and b and their corresponding groups in the statistical graphs are as follows: sham (−−−), MCAO or OGD/R (+−−), scramble (+−−), overexpression (++−), overexpression + DMSO (++−), and overexpression + TPI-1 (+++). The groups in e and f and the corresponding groups in the statistical graphs are as follows: DMSO (+−−), TPI-1 (+−+), overexpression + DMSO (++−), overexpression + TPI-1 (+++) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9010-12974_2020_1764_fig6_html.pngAnti-TNF alpha Antibody Picoband&reg;DJ-1 regulated the disassociation of NLRX1 from TRAF6 after cerebral I/R injury via SHP-1. a , g After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in rats. b , h After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in astrocytes. c , i After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. d , j After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e Immunoprecipitation and immunoblot analyses of NLRX1-TRAF6 in rats. f Immunoprecipitation and immunoblot analyses of SHP-1-TRAF6 in rats. The data are expressed as the mean ± SEM. * p < 0.05 vs. the sham group; # p < 0.05 vs. the MCAO group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group. n = 6 per group. The data are expressed as the mean ± SEM. * p < 0.05 vs. the control group; # p < 0.05 vs. the OGD/R group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group. n = 6 per group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9010-fcimb-15-1616164-g002.jpgAnti-TNF alpha Antibody Picoband&reg;Fecal microbiota transplantation (FMT) reduced systemic or local inflammatory response. (A) Protein levels of the pro-inflammatory cytokines and chemokines in the blood. (B) mRNA levels of the pro-inflammatory cytokines and chemokines in kidney tissue using real-time PCR. (C) Western blot analysis of the pro-inflammatory cytokines in kidney tissue. (D) Protein band intensities quantified using optical densitometry and normalized to β-actin levels within the respective experimental groups. n = 6. Representative images from six independent experiments. The p -values above the data denote statistical comparisons on the groups—the control (Ctrl), ischemia–reperfusion injury plus normal saline (IRI+NS), and IRI+FMT groups—which were calculated using ANOVA with Tukey’s post-hoc test. * p < 0.05; *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2025.1616164/full'>41078364</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9010-fcimb-15-1616164-g007.jpgAnti-TNF alpha Antibody Picoband&reg;Propionic acid reduced the systemic or local inflammatory response. (A) Protein levels of the pro-inflammatory cytokines and chemokines in the blood. (B) mRNA levels of the pro-inflammatory cytokines and chemokines in kidney tissue using real-time PCR. (C) Western blot analysis of the pro-inflammatory cytokines in kidney tissue. (D) Protein band intensities quantified using optical densitometry and normalized to β-actin levels within the respective experimental groups. n = 6. Representative images from six independent experiments. The p -values above the data denote statistical comparisons of the groups—the control (Ctrl), ischemia–reperfusion injury plus normal saline (IRI+NS), and IRI plus propionic acid (IRI+Prop) groups—which were calculated using ANOVA with Tukey’s post-hoc test. ** p < 0.01; *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2025.1616164/full'>41078364</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adiponectin-picoband-trade-antibody-pb9011-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9011-1-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).<br> Adiponectin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9011-2-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).<br> Adiponectin was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9011-3-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).<br> Adiponectin was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9011-4-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).<br> Adiponectin was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9011-5-IHC-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011).<br> Adiponectin was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9011-6-WB-anti-adiponectin-picoband-antibody.jpgAnti-Adiponectin/ADIPOQ Antibody Picoband&reg; Western blot analysis of Adiponectin using anti-Adiponectin antibody (PB9011). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Testis Tissue Lysate&#44;<br> Lane 2: Rat Spleen Tissue Lysate&#44;<br> Lane 3: Rat Skeletal Muscle Tissue Lysate&#44;<br> Lane 4: Rat Cardiac Muscle Tissue Lysate&#44;<br> Lane 5: Rat Liver Tissue Lysate&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Adiponectin antigen affinity purified polyclonal antibody (Catalog # PB9011) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Adiponectin at approximately 26KD. The expected band size for Adiponectin is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9011-antioxidants-13-01496-g005.pngAnti-Adiponectin/ADIPOQ Antibody Picoband&reg;Monomethyl fumarate (MMF) fully suppresses differentiation mixture (MIX)-induced protein expression of the transcription factors peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBP-α) (a) as well as the adipokines, resistin and adiponectin (b). Cellular protein abundance was assessed by Western blotting. Data are expressed as the means ± standard deviation; * p < 0.05 and ** p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.mdpi.com/2076-3921/13/12/1496'>39765824</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cntf-picoband-trade-antibody-pb9012-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9012-1-ihc-anti-cntf-picoband-antibody_1_.jpgAnti-CNTF Antibody Picoband&reg; IHC analysis of CNTF using anti-CNTF antibody (PB9012).<br> CNTF was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CNTF Antibody (PB9012) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9012-2-ihc-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; IHC analysis of CNTF using anti-CNTF antibody (PB9012).<br> CNTF was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CNTF Antibody (PB9012) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9012-3-wb-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; Western blot analysis of CNTF using anti-CNTF antibody (PB9012).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinant Human CNTF Protein 0.5ng.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody (Catalog # PB9012) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNTF at approximately 28KD. The expected band size for CNTF is at 28KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-2-picoband-trade-antibody-pb9013-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9013-1-WB-anti-il-2-antibody.jpgAnti-IL2 Antibody Picoband&reg; Western blot analysis of IL2 using anti-IL2 antibody (PB9013).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: Rat Thymus Tissue Lysate,<br> Lane 2: Rat Liver Tissue Lysate,<br> Lane 3: NRK Whole Cell Lysate,<br> Lane 4: PC12 Whole Cell Lysate,<br> Lane 5: RH35 Whole Cell Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2 antigen affinity purified polyclonal antibody (Catalog # PB9013) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2 at approximately 18KD. The expected band size for IL2 is at 18KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-18-picoband-trade-antibody-pb9014-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9014-wjg-29-2153-g001.jpgAnti-IL18 Antibody Picoband&reg;NOD-like receptor family pyrin domain-containing 3, caspase-1, and interleukin-1β were upregulated in the marginal zone and NOD-like receptor family pyrin domain-containing 3 expression was associated with hepatic alveolar echinococcosis. A: Hematoxylin and eosin staining of the lesion, marginal zone, and corresponding normal liver in hepatic alveolar echinococcosis; B: Immunohistochemical staining of NOD-like receptor family pyrin domain-containing 3 (NLRP3), caspase-1, interleukin (IL)-1β, and IL-18 in the marginal zone and corresponding normal liver; C: Relative expression of NLRP3, caspase-1, IL-1β, and IL-18; D: NLRP3 protein expression evaluated by western blotting; E: Caspase-1 protein expression evaluated by Western blotting; F: Relative protein expression levels of NLRP3 IntDen/β-actin IntDen; G: Relative protein expression levels of caspase-1 IntDen/β-actin IntDen. C, n = 60. Scale bar: 50 μm. Western blotting was performed in triplicate (mean ± SD). a P < 0.05; b P < 0.01. HAE: Hepatic alveolar echinococcosis; IOD: Integral Optical Density; NLRP3: NOD-like receptor family pyrin domain-containing 3. IL-1β: Interleukin-1β; IL-18: Interleukin-18.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10130966/&orig_host=www.ncbi.nlm.nih.gov'>37122606</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9014-wjg-29-2153-g003.jpgAnti-IL18 Antibody Picoband&reg;Reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3 inflammasome activation in hepatic alveolar echinococcosis rats. A: Reactive oxygen species (ROS) production in the 20, 50, and 100 mg/kg/d N-tert-Butyl-α-phenylnitrone (PBN) groups; B: ROS production in the 50 mg/kg/d PBN, normal saline (NS), and control groups; C: Analysis of inflammation; D: Immunohistochemical staining of NLRP3, caspase-1, interleukin (IL)-1β, and IL-18; E: Relative expression of NLRP3, caspase-1, IL-1β, and IL-18 in the marginal zone compared with the corresponding normal liver; F: Western blotting analysis of the PBN, NS, and control groups; G: NLRP3 IntDen/β-actin IntDen protein expression; H: Caspase-1 IntDen/β-actin IntDen protein expression; I: Relationship between ROS production and the relative expression of NLRP3; J: The relative expression of NLRP3 and inflammation observed in the marginal zone. Western blotting was performed in triplicate (mean ± SD). Scale bar, 50 μm. A, n = 3 rats per group; B, C, and E, n = 10 rats per group; I and J, n = 10. a P < 0.05; b P < 0.01; c P < 0.001; d P < 0.0001. NLRP3: NOD-like receptor family pyrin domain-containing 3; ROS: Reactive oxygen species; PBN: N-tert-Butyl-α-phenylnitrone; NS: Normal saline; IOD: Integral optical density; IL-1β: Interleukin-1β; IL-18: Interleukin-18.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10130966/&orig_host=www.ncbi.nlm.nih.gov'>37122606</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9014-wjg-29-2153-g005.jpgAnti-IL18 Antibody Picoband&reg;Reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3-caspase-1-interleukin-18 pathway activation in Kupffer cells. A: Reactive oxygen species (ROS) production with the indicated N-acetyl-L-cysteine (NAC) dose at the specified time points. b P < 0.01, blank vs 5 mmol/L; a P < 0.05, 10 mmol/L vs 5 mmol/L. b P < 0.0120 mmol/L vs 5 mmol/L; B: Representation of the Transwell model used in this study; C: ROS production in the co-culture groups treated with or without NAC at the indicated time points; D: Relative production of ROS; E: Interleukin (IL)-18 expression; F: IL-1β expression; G: Apoptosis of hepatocytes in the co-culture groups treated with or without NAC at 24, 48, and 72 h; H: Cell viability; I: Western blotting analysis of the indicated proteins; J: NLRP3 IntDen/β-actin IntDen protein expression; K: Caspase-1 IntDen/β-actin IntDen protein expression. a P < 0.05; b P < 0.01. NLRP3: NOD-like receptor family pyrin domain-containing 3; ROS: Reactive oxygen species; FITC-A: Fluorescein isothiocyanate isomer I-A; PI: Propidium Iodide; NAC: N-acetyl-L-cysteine; IL-1β: Interleukin-1β; IL-18: Interleukin-18.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10130966/&orig_host=www.ncbi.nlm.nih.gov'>37122606</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9014-il18-primary-antibodies-wb-testing-1.jpgAnti-IL18 Antibody Picoband&reg;Western blot analysis of IL18 using anti-IL18 antibody (PB9014). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: recombinant mouse IL18 protein lysates,<br> Lane 2: rat PC-12 whole cell lysates,<br> Lane 3: rat C6 whole cell lysates,<br> Lane 4: mouse RAW264.7 whole cell lysates,<br> Lane 5: mouse ANA-1 whole cell lysates,<br> Lane 6: mouse J774.1 whole cell lysates,<br> Lane 7: mouse ID8 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9014-il18-primary-antibodies-ihc-testing-1.jpgAnti-IL18 Antibody Picoband&reg;IHC analysis of IL18 using anti-IL18 antibody (PB9014). <br> IL18 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (PB9014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-brca1-picoband-trade-antibody-pb9015-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-wb-testing-1.jpgAnti-BRCA1 Antibody Picoband&reg; Western blot analysis of BRCA1 using anti-BRCA1 antibody (PB9015). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human T-47D whole cell lysates,<br> Lane 3: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # PB9015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 290 kDa. The expected band size for BRCA1 is at 208 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-ihc-testing-2.jpgAnti-BRCA1 Antibody Picoband&reg; IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015). <br> BRCA1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BRCA1 Antibody (PB9015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-ihc-testing-3.jpgAnti-BRCA1 Antibody Picoband&reg; IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015). <br> BRCA1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BRCA1 Antibody (PB9015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-ihc-testing-4.jpgAnti-BRCA1 Antibody Picoband&reg; IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015). <br> BRCA1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BRCA1 Antibody (PB9015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-fcm-testing-8.jpgAnti-BRCA1 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-BRCA1 antibody (PB9015). <br>Overlay histogram showing HepG2 cells stained with PB9015 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BRCA1 Antibody (PB9015, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-ihc-testing-5.jpgAnti-BRCA1 Antibody Picoband&reg; IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015). <br> BRCA1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BRCA1 Antibody (PB9015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-ihc-testing-6.jpgAnti-BRCA1 Antibody Picoband&reg; IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015). <br> BRCA1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BRCA1 Antibody (PB9015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9015-brca1-primary-antibodies-ihc-testing-7.jpgAnti-BRCA1 Antibody Picoband&reg; IHC analysis of BRCA1 using anti-BRCA1 antibody (PB9015). <br> BRCA1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BRCA1 Antibody (PB9015) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egfr-picoband-trade-antibody-pb9016-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9016-1-WB-anti-egfr-picoband-antibody.jpgAnti-EGFR Antibody Picoband&reg; Western blot analysis of EGFR using anti-EGFR antibody (PB9016).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: A549 Whole Cell Lysate,<br> Lane 3: A431 Whole Cell Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # PB9016) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 200KD. The expected band size for EGFR is at 134KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9016-egfr-primary-antibodies-if-testing-2.jpgAnti-EGFR Antibody Picoband&reg; IF analysis of EGFR using anti-EGFR antibody (PB9016). <br> EGFR was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EGFR Antibody (PB9016) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9016-egfr-primary-antibodies-wb-testing-3.pngAnti-EGFR Antibody Picoband&reg; Western blot analysis of EGFR using anti-EGFR antibody (PB9016). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw) for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antibody (PB9016) at 1 ug/mL in 5% milk/PBS/0.05% Tween 20 overnight at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit antibody conjugated with HRP at 1:5,000 in 5% milk/PBS/Tw at 4℃ for 12 hours. The signal is developed using an SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific). A specific band was detected for EGFR at approximately 134 kDa. The expected band size for EGFR is at 134 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-icam1-picoband-trade-antibody-pb9017-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9017-1-IHC-anti-icam-1-antibody.jpgAnti-ICAM1 Antibody Picoband&reg; IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9017).<br> ICAM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9017-2-WB-anti-icam-1-antibody.jpgAnti-ICAM1 Antibody Picoband&reg; Western blot analysis of ICAM1 using anti-ICAM1 antibody (PB9017).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: HEPG2 Whole Cell Lysate,<br> Lane 2: K562 Whole Cell Lysate,<br> Lane 3: A549 Whole Cell Lysate,<br> Lane 4: HT1080 Whole Cell Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # PB9017) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 100KD. The expected band size for ICAM1 is at 58KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9017-3.jpgAnti-ICAM1 Antibody Picoband&reg; IF analysis of ICAM1 using anti-ICAM1 antibody (PB9017) <br> ICAM1 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9017-4.jpgAnti-ICAM1 Antibody Picoband&reg; IF analysis of ICAM1 using anti-ICAM1 antibody (PB9017) <br> ICAM1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ICAM1 Antibody (PB9017) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9017-5.pngAnti-ICAM1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ICAM1 antibody (PB9017). <br> Overlay histogram showing A431 cells stained with PB9017 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ICAM1 Antibody (PB9017&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-icam1-picoband-trade-antibody-pb9018-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9018-icam1-primary-antibodies-wb-testing-1.jpgAnti-ICAM1 Antibody Picoband&reg; Western blot analysis of ICAM1using anti-ICAM1 antibody (PB9018). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat NRK whole cell lysates, <br> Lane 2: mouse spleen tissue lysates, <br> Lane 3: mouse liver tissue lysates, <br> Lane 4: mouse kidney tissue lysates, <br> Lane 5: mouse ANA-1 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 antigen affinity purified polyclonal antibody (Catalog # PB9018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICAM1 at approximately 90-100KD. The expected band size for ICAM1 is at 58KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9018-icam1-primary-antibodies-ihc-testing-2.jpgAnti-ICAM1 Antibody Picoband&reg; IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018). <br> ICAM1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9018-icam1-primary-antibodies-ihc-testing-3.jpgAnti-ICAM1 Antibody Picoband&reg; IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018). <br> ICAM1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9018-icam1-primary-antibodies-ihc-testing-4.jpgAnti-ICAM1 Antibody Picoband&reg; IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018). <br> ICAM1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9018-icam1-primary-antibodies-ihc-testing-5.jpgAnti-ICAM1 Antibody Picoband&reg; IHC analysis of ICAM1 using anti-ICAM1 antibody (PB9018). <br> ICAM1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ICAM1 Antibody (PB9018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serum-amyloid-p-picoband-trade-antibody-pb9020-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9020-serum-amyloid-p-primary-antibodies-wb-testing-1.jpgAnti-Serum Amyloid P/APCS Antibody Picoband&reg; Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9020). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB9020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 25-29 kDa. The expected band size for Serum Amyloid P is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9020-serum-amyloid-p-primary-antibodies-ihc-testing-2.jpgAnti-Serum Amyloid P/APCS Antibody Picoband&reg; IHC analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9020). <br> Serum Amyloid P was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Serum Amyloid P Antibody (PB9020) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serum-amyloid-p-picoband-trade-antibody-pb9021-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9021-1-IHC-anti-sap-ptx2-antibody.jpgAnti-Serum Amyloid P/APCS Antibody Picoband&reg; IHC analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9021). Serum Amyloid P was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Serum Amyloid P Antibody (PB9021) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9021-2_1.jpgAnti-Serum Amyloid P/APCS Antibody Picoband&reg; Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB9021). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates&#44; <br> Lane 2: mouse liver tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB9021) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 29KD. The expected band size for Serum Amyloid P is at 25KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccl3-picoband-trade-antibody-pb9022-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9022-1-WB-anti-ccl3-mip-1alpha-picoband-antibody.jpgAnti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband&reg; Western blot analysis of CCL3 using anti-CCL3 antibody (PB9022).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinant Rat CCL3 Protein 0.5ng.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9022) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 33KD. The expected band size for CCL3 is at 33KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccl18-picoband-trade-antibody-pb9023-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9023-1-WB-anti-ccl18-parc-antibody.jpgAnti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband&reg; Western blot analysis of CCL18 using anti-CCL18 antibody (PB9023).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinant Human CCL18 Protein 0.5ng.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL18 antigen affinity purified polyclonal antibody (Catalog # PB9023) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL18 at approximately 19KD. The expected band size for CCL18 is at 19KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9023-ccl18-primary-antibodies-elisa-testing-2.jpgAnti-Macrophage Inflammatory Protein 4/CCL18 Antibody Picoband&reg; Sandwich ELISA - Recombinant human CCL18/PARC protein standard curve.<br> Use in combination with reagents from Human CCL18/PARC ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0686). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ifn-gamma-picoband-trade-antibody-pb9024-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9024-1-WB-anti-ifn-gamma-antibody.jpgAnti-Interferon gamma/IFNG Antibody Picoband&reg; Western blot analysis of Interferon gamma using anti-Interferon gamma antibody (PB9024).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinant Rat Interferon gamma Protein 0.5ng.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Interferon gamma antigen affinity purified polyclonal antibody (Catalog # PB9024) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Interferon gamma at approximately 19KD. The expected band size for Interferon gamma is at 19KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-1-beta-picoband-trade-antibody-pb9025-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-fphar-14-1181133-g004.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;The pretreatment of RD-6 exerts anti-inflammatory effects against indomethacin-induced GU rats. The levels of IL-1β (A) , IL-6 (B) , IL-17 (C) , and PGE2 (D) in gastric tissue. Data are expressed as mean ± S.E.M ( n = 6). One-way ANOVA with the uncorrected Fisher’s LSD test was used to evaluate multiple comparisons. # p < 0.05, and ## p < 0.01 vs. the NC group; * p < 0.05, and ** p < 0.01 vs. the IND group. NC, normal control; IND, indomethacin; RAN, ranitidine; RD-6-L, M, and H represent Ruda-6 at low, medium and high doses, respectively.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10449537/&orig_host=www.ncbi.nlm.nih.gov'>37637418</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-fphar-14-1181133-g006.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;The pretreatment of RD-6 inhibited the IL-17 signaling pathway in indomethacin-induced GU rats. The expression of IL17RA, FOS, IL1B, and PTGS2 determined in gastric tissue by qRT-PCR (A) and western bloting (B) . Data are expressed as mean ± S.E.M ( n = 3). One-way ANOVA with the uncorrected Fisher’s LSD test was used to evaluate multiple comparisons. # p < 0.05, ## p < 0.01 vs. NC group; * p < 0.05, ** p < 0.01 vs. IND group. NC, normal control; IND, indomethacin; RD-6-L, M, and H represent Ruda-6 at low, medium and high doses, respectively.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10449537/&orig_host=www.ncbi.nlm.nih.gov'>37637418</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-wjg-29-2153-g001.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;NOD-like receptor family pyrin domain-containing 3, caspase-1, and interleukin-1β were upregulated in the marginal zone and NOD-like receptor family pyrin domain-containing 3 expression was associated with hepatic alveolar echinococcosis. A: Hematoxylin and eosin staining of the lesion, marginal zone, and corresponding normal liver in hepatic alveolar echinococcosis; B: Immunohistochemical staining of NOD-like receptor family pyrin domain-containing 3 (NLRP3), caspase-1, interleukin (IL)-1β, and IL-18 in the marginal zone and corresponding normal liver; C: Relative expression of NLRP3, caspase-1, IL-1β, and IL-18; D: NLRP3 protein expression evaluated by western blotting; E: Caspase-1 protein expression evaluated by Western blotting; F: Relative protein expression levels of NLRP3 IntDen/β-actin IntDen; G: Relative protein expression levels of caspase-1 IntDen/β-actin IntDen. C, n = 60. Scale bar: 50 μm. Western blotting was performed in triplicate (mean ± SD). a P < 0.05; b P < 0.01. HAE: Hepatic alveolar echinococcosis; IOD: Integral Optical Density; NLRP3: NOD-like receptor family pyrin domain-containing 3. IL-1β: Interleukin-1β; IL-18: Interleukin-18.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10130966/&orig_host=www.ncbi.nlm.nih.gov'>37122606</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-wjg-29-2153-g003.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;Reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3 inflammasome activation in hepatic alveolar echinococcosis rats. A: Reactive oxygen species (ROS) production in the 20, 50, and 100 mg/kg/d N-tert-Butyl-α-phenylnitrone (PBN) groups; B: ROS production in the 50 mg/kg/d PBN, normal saline (NS), and control groups; C: Analysis of inflammation; D: Immunohistochemical staining of NLRP3, caspase-1, interleukin (IL)-1β, and IL-18; E: Relative expression of NLRP3, caspase-1, IL-1β, and IL-18 in the marginal zone compared with the corresponding normal liver; F: Western blotting analysis of the PBN, NS, and control groups; G: NLRP3 IntDen/β-actin IntDen protein expression; H: Caspase-1 IntDen/β-actin IntDen protein expression; I: Relationship between ROS production and the relative expression of NLRP3; J: The relative expression of NLRP3 and inflammation observed in the marginal zone. Western blotting was performed in triplicate (mean ± SD). Scale bar, 50 μm. A, n = 3 rats per group; B, C, and E, n = 10 rats per group; I and J, n = 10. a P < 0.05; b P < 0.01; c P < 0.001; d P < 0.0001. NLRP3: NOD-like receptor family pyrin domain-containing 3; ROS: Reactive oxygen species; PBN: N-tert-Butyl-α-phenylnitrone; NS: Normal saline; IOD: Integral optical density; IL-1β: Interleukin-1β; IL-18: Interleukin-18.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10130966/&orig_host=www.ncbi.nlm.nih.gov'>37122606</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-wjg-29-2153-g005.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;Reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3-caspase-1-interleukin-18 pathway activation in Kupffer cells. A: Reactive oxygen species (ROS) production with the indicated N-acetyl-L-cysteine (NAC) dose at the specified time points. b P < 0.01, blank vs 5 mmol/L; a P < 0.05, 10 mmol/L vs 5 mmol/L. b P < 0.0120 mmol/L vs 5 mmol/L; B: Representation of the Transwell model used in this study; C: ROS production in the co-culture groups treated with or without NAC at the indicated time points; D: Relative production of ROS; E: Interleukin (IL)-18 expression; F: IL-1β expression; G: Apoptosis of hepatocytes in the co-culture groups treated with or without NAC at 24, 48, and 72 h; H: Cell viability; I: Western blotting analysis of the indicated proteins; J: NLRP3 IntDen/β-actin IntDen protein expression; K: Caspase-1 IntDen/β-actin IntDen protein expression. a P < 0.05; b P < 0.01. NLRP3: NOD-like receptor family pyrin domain-containing 3; ROS: Reactive oxygen species; FITC-A: Fluorescein isothiocyanate isomer I-A; PI: Propidium Iodide; NAC: N-acetyl-L-cysteine; IL-1β: Interleukin-1β; IL-18: Interleukin-18.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10130966/&orig_host=www.ncbi.nlm.nih.gov'>37122606</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-12974_2020_1764_fig2_html.pngAnti-IL1 beta/IL1B Antibody Picoband&reg;DJ-1 interference increased the expression of TNF-α, IL-1β, and IL-6 after cerebral I/R injury. a and c Western blot detecting DJ-1 and the cytokines TNF-α, IL-1β, and IL-6 in rats. b , d Western blot detecting DJ-1 and the cytokines TNF-α, IL-1β, and IL-6 in astrocytes. e–g Quantification of TNF-α, IL-1β, and IL-6 in rats by ELISA. h‑j Quantification of TNF-α, IL-1β, and IL-6 in astrocytes by ELISA. The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 6 per group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-12974_2020_1764_fig5_html.pngAnti-IL1 beta/IL1B Antibody Picoband&reg;DJ-1 inhibited the expression of TNF-α, IL-1β, and IL-6 after cerebral I/R injury via SHP-1. a , c After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. b , d After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e , g After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. f , h After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. i , k , m Quantification of TNF-α, IL-1β, and IL-6 in rats by ELISA. j , l , n Quantification of TNF-α, IL-1β, and IL-6 in astrocytes by ELISA. The data are expressed as the mean ± SEM. * p < 0.05 vs. the sham group; # p < 0.05 vs. the MCAO group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group; ◆ p < 0.05 vs. the TPI-1 group. n = 6 per group. The data are expressed as the mean ± SEM. * p < 0.05 vs. the control group; # p < 0.05 vs. the OGD/R group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group; ◆ p < 0.05 vs. the TPI-1 group. n = 6 per group. The groups in a and b and their corresponding groups in the statistical graphs are as follows: sham (−−−), MCAO or OGD/R (+−−), scramble (+−−), overexpression (++−), overexpression + DMSO (++−), and overexpression + TPI-1 (+++). The groups in e and f and the corresponding groups in the statistical graphs are as follows: DMSO (+−−), TPI-1 (+−+), overexpression + DMSO (++−), overexpression + TPI-1 (+++) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-12974_2020_1764_fig6_html.pngAnti-IL1 beta/IL1B Antibody Picoband&reg;DJ-1 regulated the disassociation of NLRX1 from TRAF6 after cerebral I/R injury via SHP-1. a , g After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in rats. b , h After virus and TPI-1 were used to overexpress DJ-1 and inhibit SHP-1, respectively, Western blotting was used to detect NLRX1, TRAF6, and SHP-1 in astrocytes. c , i After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in rats. d , j After treatment with an SHP-1 inhibitor, Western blotting was used to detect the cytokines IL-1β, IL-6, and TNF-α in astrocytes. e Immunoprecipitation and immunoblot analyses of NLRX1-TRAF6 in rats. f Immunoprecipitation and immunoblot analyses of SHP-1-TRAF6 in rats. The data are expressed as the mean ± SEM. * p < 0.05 vs. the sham group; # p < 0.05 vs. the MCAO group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group. n = 6 per group. The data are expressed as the mean ± SEM. * p < 0.05 vs. the control group; # p < 0.05 vs. the OGD/R group; & p < 0.05 vs. the overexpression group; ★ p < 0.05 vs. the DMSO group; △ p < 0.05 vs. the DMSO group. n = 6 per group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-020-01764-x'>32151250</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-fsn3-13-e70736-g007.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;SIRT1 inhibitor EX‐527 increased M1‐like macrophage and lipid accumulation by EGCG in the model cells. (A) Determination of the optimal concentration of EX‐527. n = 3. (B) Representative WB images and quantitative analysis of the level of iNOS, Arg‐1, SIRT1, IL‐4, P‐STAT6, and P‐AKT1 in the RAW264.7 cells. n = 3 (C) Representative WB images and quantitative analysis of the level of SREBP‐1 and SREBP‐2 in the MPC5 cells. n = 3. (D) Levels of TNF‐α, IL‐18, and IL‐1β in supernatant. n = 6. (E, F) Levels of IL‐4 and IL‐10 in supernatant. n = 6. Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the EGCG group, c p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-fsn3-13-e70736-g010.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;TP corrected inflammation levels in the aging with DKD model rats. (A) Representative WB images and quantification of the expression of TNF‐α, IL‐1β, and IL‐18 ( n = 6). (B–D) Levels of TNF‐α, IL‐18, and IL‐1β in serum ( n = 6). (E) Representative WB images and quantification of the expression of IL‐4 and IL‐10 ( n = 6). (F, G) Levels of IL‐4 and IL‐10 in serum ( n = 6). Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the TP‐L group, c p < 0.05; compared with the TP‐M group, d p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-fsn3-13-e70736-g011.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg;SIRT1 agonist SRT1720 increased M2‐like macrophage and decreased lipid deposition by EGCG in the model cells. (A) Determination of the optimal concentration of SRT1720 ( n = 3). (B) Representative WB images and quantitative analysis of the level of iNOS, Arg‐1, SIRT1, IL‐4, P‐STAT6, and P‐AKT1 in the RAW264.7 cells ( n = 3). (C) Representative WB images and quantitative analysis of the level of SREBP‐1 and SREBP‐2 in the MPC5 cells ( n = 3). (D) Levels of TNF‐α, IL‐18, and IL‐1β in supernatant ( n = 6). (E, F) Levels of IL‐4 and IL‐10 in supernatant ( n = 6). Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the EGCG group, c p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-il1b-primary-antibodies-wb-testing-1.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg; Western blot analysis of IL1 beta using anti-IL1 beta antibody (PB9025). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant rat IL1 beta protein 10 ng,<br> Lane 2: recombinant rat IL1 beta protein 5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # PB9025) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-il1b-primary-antibodies-wb-testing-2.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg; Western blot analysis of IL1 beta using anti-IL1 beta antibody (PB9025). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse RAW264.7 whole cell lysates,<br> Lane 2: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL1 beta antigen affinity purified polyclonal antibody (Catalog # PB9025) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL1 beta at approximately 35 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-il1b-primary-antibodies-ihc-testing-3.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg; IHC analysis of IL1 beta using anti-IL1 beta antibody (PB9025). <br>IL1 beta was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL1 beta Antibody (PB9025) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9025-il1b-primary-antibodies-ihc-testing-4.jpgAnti-IL1 beta/IL1B Antibody Picoband&reg; IHC analysis of IL1 beta using anti-IL1 beta antibody (PB9025). <br>IL1 beta was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL1 beta Antibody (PB9025) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ki67-picoband-trade-antibody-pb9026-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9026-1-IHC-anti-ki67-picoband-antibody.jpgAnti-Ki67/MKI67 Antibody Picoband&reg; IHC analysis of Ki67 using anti-Ki67 antibody (PB9026).<br> Ki67 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9026-2-WB-anti-ki67-picoband-antibody.jpgAnti-Ki67/MKI67 Antibody Picoband&reg; Western blot analysis of Ki67 using anti-Ki67 antibody (PB9026).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: MCF-7 Whole Cell Lysate,<br> Lane 3: COLO320 Whole Cell Lysate,<br> Lane 4: HEPG2 Whole Cell Lysate,<br> Lane 5: SKOV Whole Cell Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ki67 antigen affinity purified polyclonal antibody (Catalog # PB9026) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ki67 at approximately 358KD. The expected band size for Ki67 is at 358KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-41467_2023_41529_fig5_html.pngAnti-Ki67/MKI67 Antibody Picoband&reg;Therapeutic potentials of Pg-Fe-hMSC in both monocellular and multicellular humanized fibrotic models. a Schematic illustration and representative image of EpCAM immunostaining in primary human lung epithelial cells (hLECs). Scale bar, 50 μm. b Mitochondrial transfer rates from the indicated hMSC to the primary hLEC ( n = 3 biologically independent cells). c Relative intracellular ROS levels ( n = 3 biologically independent cells), d TGF- β expression levels ( n = 4 biologically independent cells), and e Viability of BLM-treated hLEC after the indicated treatment using different engineered hMSCs ( n = 3 biologically independent cells). f Schematic illustration showing the preparation of the 3D multicellular human fibrotic model. g Representative immunostaining images showing the expression of α -smooth muscle actin ( α -SMA), vimentin, collagen-I, and Ki-67 in the healthy and fibrotic multicellular human spheroid models. Blue fluorescent signals indicate the cell nuclei and green signals indicate the biomarkers. Scale bar, 20 μm. h Mitochondrial transfer rates of different engineered hMSC in fibrotic human lung spheroids ( n = 3 biologically independent experiments). i Relative intracellular ROS levels ( n = 3 biologically independent experiments) and j TGF- β expression levels of fibrotic human lung spheroids after the indicated treatment using different engineered hMSCs ( n = 4 biologically independent experiments). k Viability of fibrotic human lung spheroids after the indicated treatment using different engineered hMSCs ( n = 3 biologically independent experiments). Data are presented as means ± SD. Statistical significance was analyzed using ordinary one-way ANOVA. ECs epithelial cells. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-023-41529-7'>37723135</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-ki67-primary-antibodies-if-testing-3.pngAnti-Ki67/MKI67 Antibody Picoband&reg; IF analysis of Ki67 using anti-Ki67 antibody (PB9026). <br> Ki67 was detected in paraffin-embedded section of human colon organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-jcav14p3404g002.jpgAnti-Ki67/MKI67 Antibody Picoband&reg;miR-134-3p overexpression inhibits HuOCSC activity both in vitro and in vivo . (A) Proliferation inhibition rate assay (n = 3). ** P < 0.01 vs. miR-mut. t test. (B) Cell cycle assay (n = 3). * P < 0.05 vs. miR-mut; ** P < 0.01 vs. miR-mut. t test. (C) Transwell assay of migration ability of HuOCSCs (n = 3). ** P < 0.01 vs. miR-mut. t test. (D) Angiogenesis assay to detect the ability of formation of tubular 3D structures (n = 3). ** P < 0.01 vs. miR-mut. t test. (E) In vitro graft tumor assay. * P < 0.05 vs. miR-mut. t test. (F) The weight and volume of graft tumors. * P < 0.05 vs. miR-mut. t test. (G) HE staining (2× magnification). (H) Immunofluorescence labeling of Ki67 (400× magnification).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10647200/&orig_host=www.ncbi.nlm.nih.gov'>38021163</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-ki67-primary-antibodies-icc-testing-4.jpgAnti-Ki67/MKI67 Antibody Picoband&reg; ICC analysis of Ki67 using anti-Ki67 antibody (PB9026). <br> Ki67 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-13058_2024_1858_fig5_html.pngAnti-Ki67/MKI67 Antibody Picoband&reg;Silencing TFAP2A inhibits proliferation of TNBC cells. Expression of TFAP2A was knocked down by siRNAs in MDA-MB-231 and BT-549 cells, cell proliferation was assessed by A MTS assay, B Colony formation assay, and C EdU assay. D Western blot analysis showing the expression levels of Ki67, TFAP2A, and the phosphorylation levels of ERK and AKT in MDA-MB-231 cells and E BT-549 cells with or without TFAP2A knockdown <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13058-024-01858-x'>38890750</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-13058_2024_1858_fig1_html.pngAnti-Ki67/MKI67 Antibody Picoband&reg;miR-8072 suppresses TNBC tumor progression and predicts favorable prognosis. ( A ) Serum levels of miR-8072 in breast cancer, benign breast disease, and non-cancer samples were analyzed using data from GEO (GSE73002) (** P < 0.01) . B The relationships between miR-8072 level and overall survival (OS) in breast cancer patients were analyzed using public database Kaplan–Meier plotter ( ). 1062 breast cancer patients were included, 525 cases were in miR-8072 low-expression cohort, and 537 cases were in miR-8072 high-expression cohort. The median survival for the high-expression cohort of miR-8072 is 115.4 months, while it is 148.53 months for the low-expression cohort of miR-8072 (upper panel). 97 TNBC patients were included for analysis. Among them, 28 cases were in miR-8072 low-expression cohort, and 69 cases were in miR-8072 high-expression cohort. The upper quartile survival for cohorts with high-expression of miR-8072 is 98.83 months, while it is 36.43 months for cohorts with low-expression of miR-8072 (lower panel). C MDA-MB-231 cells with stably overexpressed miR-8072 or control (Ctrl) cells were subcutaneously implanted in nude mice, tumor volume were measured every 7 days. D Tumor nodules were collected and weighed 35 days later after implantation. E Expression of Ki67 in the tumor nodules was detected by immunohistochemistry <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13058-024-01858-x'>38890750</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-ki67-primary-antibodies-if-testing-4.pngAnti-Ki67/MKI67 Antibody Picoband&reg;IF analysis of Ki67 using anti-Ki67 antibody (PB9026). <br> Ki67 was detected in a paraffin-embedded section ofNude mouse tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-ki67-primary-antibodies-icc-testing-1.jpgAnti-Ki67/MKI67 Antibody Picoband&reg;ICC/IF analysis of Ki67 using anti-Ki67 antibody (PB9026). <br> Ki67 was detected in an immunocytochemical section of human Hela cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with rabbit anti-Ki67 Antibody (PB9026) at a dilution of 1:50 overnight at 4°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-ki67-primary-antibodies-icc-testing-2.jpgAnti-Ki67/MKI67 Antibody Picoband&reg;ICC/IF analysis of Ki67 using anti-Ki67 antibody (PB9026). <br> Ki67 was detected in an immunocytochemical section of human SIHA cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and then treated with a membrane permeabilization agent (AR0205) for 5 minutes.The cells were blocked with 10% goat serum. And then incubated with rabbit anti-Ki67 Antibody (PB9026) at a dilution of 1:50 overnight at 4°C. DyLight®488 conjugated goat Anti-rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9026-ki67-primary-antibodies-ihc-testing-1.pngAnti-Ki67/MKI67 Antibody Picoband&reg; IHC analysis of Ki67 using anti-Ki67 antibody (PB9026).<br> Ki67 was detected in paraffin-embedded section of nude mouse tumor tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:1000 rabbit anti-Ki67 Antibody (PB9026) overnight at 4°C. Ready-to-use SABC-POD kit (rabbit IgG) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-goat-anti-mouse-igg-secondary-antibody-ba1001-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1001-1-1-IHC-goat-anti-mouse-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Mouse IgG (H+L) Secondary Antibody, Biotin ConjugatedNEFH was detected in paraffin-embedded sections of rat brain tissues using mouse anti-NEFH Antigen Affinity purified monoclonal antibody (Catalog # MA1071) at 1 μg/mL. Biotin Conjugated goat anti-mouse IgG (H+L) secondary antibody (Catalog # BA1001) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1001-2_1-IHC-goat-anti-mouse-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Mouse IgG (H+L) Secondary Antibody, Biotin ConjugatedPCNA was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti-PCNA Antigen Affinity purified monoclonal antibody (Catalog # MA1083) at 1 μg/mL. Biotin Conjugated goat anti-mouse IgG (H+L) secondary antibody (Catalog # BA1001) was used to detect the primary antibody at 10μg/mL. https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-goat-anti-rabbit-igg-secondary-antibody-ba1003-boster.html2026-03-24T05:04:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-2_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedMNAT1 was detected in paraffin-embedded sections of mouse testis tissues using rabbit anti-MNAT1 Antigen Affinity purified polyclonal antibody (Catalog # PB9614) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-8_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedNucleophosmin was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-Nucleophosmin Antigen Affinity purified polyclonal antibody (Catalog # PB9341) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-4_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedSLC10A1 was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti-SLC10A1 Antigen Affinity purified polyclonal antibody (Catalog # PB9745) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-1_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedAquaporin 1 was detected in paraffin-embedded sections of mouse kidney tissues using rabbit anti-Aquaporin 1 Antigen Affinity purified polyclonal antibody (Catalog # PB9473) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-5_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedABCB11 was detected in paraffin-embedded sections of rat liver tissues using rabbit anti-ABCB11 Antigen Affinity purified polyclonal antibody (Catalog # PB9414) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-7_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedHLA A was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-HLA A Antigen Affinity purified polyclonal antibody (Catalog # PB9376) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-3_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedRbAp48 was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti-RbAp48 Antigen Affinity purified polyclonal antibody (Catalog # PB9797) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1003-6_1-IHC-goat-anti-rabbit-igg-secondary-antibody-biotin-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedAquaporin 2 was detected in paraffin-embedded sections of rat kidney tissues using rabbit anti-Aquaporin 2 Antigen Affinity purified polyclonal antibody (Catalog # PB9474) at 1 μg/mL. Biotin Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1003) was used to detect the primary antibody at 10μg/mL. https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-goat-anti-mouse-igm-secondary-antibody-ba1004-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGoat Anti-Mouse IgM Secondary Antibody, Biotin ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-rabbit-anti-rat-igg-secondary-antibody-ba1005-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Rat IgG (H+L) Secondary Antibody, Biotin ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-rabbit-anti-goat-igg-secondary-antibody-ba1006-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Goat IgG (H+L) Secondary Antibody, Biotin ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-rabbit-anti-human-igg-secondary-antibody-ba1020-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Human IgG (H+L) Secondary Antibody, Biotin ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-goat-anti-human-igm-secondary-antibody-ba1022-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGoat Anti-Human IgM Secondary Antibody, Biotin ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-protein-a-ba1025-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBiotin Conjugated Protein ABoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-protein-g-ba1026-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngBiotin Conjugated Protein GBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-mouse-anti-human-igg-secondary-antibody-bm2001-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse Anti-Human IgG (H+L) Secondary Antibody, Biotin ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/biotin-conjugated-mouse-anti-rabbit-igg-secondary-antibody-bm2004-boster.html2026-03-24T05:04:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse Anti-Rabbit IgG (H+L) Secondary Antibody, Biotin ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/cy3-conjugated-goat-anti-mouse-igg-secondary-antibody-ba1031-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1031.jpgGoat Anti-Mouse IgG (H+L) Secondary Antibody, Cy3 ConjugatedFLOT2 was detected in an immunocytochemical section of SiHa cells using mouse anti-FLOT2 Antigen Affinity purified monoclonal antibody (Catalog # M06107-2). Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used to detect the primary antibody. https://www.bosterbio.com/products/all-secondary-antibodies/cy3-conjugated-goat-anti-rabbit-igg-secondary-antibody-ba1032-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1032.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Cy3 ConjugatedGFAP was detected in a paraffin-embedded section of rat brain tissues using rabbit anti-GFAP Antigen Affinity purified polyclonal antibody (Catalog # PB9082). Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used to detect the primary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1032-1_1.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Cy3 ConjugatedPCNA was detected in immunocytochemical section of A549 cells using rabbit anti-PCNA Antigen Affinity purified polyclonal antibody (Catalog # A00125). Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used to detect the primary antibody. https://www.bosterbio.com/products/all-secondary-antibodies/cy3-conjugated-rabbit-anti-goat-igg-secondary-antibody-ba1034-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Goat IgG (H+L) Secondary Antibody, Cy3 ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/cy3-conjugated-rabbit-anti-human-igg-secondary-antibody-ba1035-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Human IgG (H+L) Secondary Antibody, Cy3 ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/cy3-conjugated-avidin-ba1037-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1037.jpgCy3 Conjugated AvidinCD5 was detected in a paraffin-embedded section of human tonsil tissues using rabbit anti-CD5 Antigen Affinity purified polyclonal antibody (Catalog # A00480-2). Biotin conjugated goat anti-rabbit IgG (BA1003) was used to detect the primary antibody. Cy3 Conjugated Avidin (BA1037) was used for visualization.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1037-1_1.jpgCy3 Conjugated AvidinCD68 was detected in a paraffin-embedded section of human tonsil tissues using mouse anti-CD68 Antigen Affinity purified monoclonal antibody (Catalog # M00602). Biotin conjugated goat anti-mouse IgG (BA1001) was used to detect the primary antibody. Cy3 Conjugated Avidin (BA1037) was used for visualization. https://www.bosterbio.com/products/all-secondary-antibodies/dylight-reg-488-conjugated-goat-anti-mouse-igg-ba1126-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1126_2.jpgGoat Anti-Mouse IgG (H+L) Secondary Antibody, Fluoro488 ConjugatedAlpha-Smooth Muscle Actin was detected in paraffin-embedded sections of rat lung tissues using mouse anti-Alpha-Smooth Muscle Actin Antigen Affinity purified monoclonal antibody (Catalog # MA1106). Fluoro488 Conjugated Goat Anti-Mouse IgG (BA1126) was used to detect the primary antibody. https://www.bosterbio.com/products/all-secondary-antibodies/dylight-reg-488-conjugated-goat-anti-rabbit-igg-ba1127-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1127_1.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro488 ConjugatedEPCAM was detected in a paraffin-embedded section of rat colon tissues using rabbit anti-EPCAM Antigen Affinity purified polyclonal antibody (Catalog # A00276-1). Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used to detect the primary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1127-1_2_1.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, Fluoro488 ConjugatedPRKCG was detected in a paraffin-embedded section of rat celebellum tissues using rabbit anti-PRKCG Antigen Affinity purified polyclonal antibody (Catalog # A01890). Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used to detect the primary antibody. https://www.bosterbio.com/products/all-secondary-antibodies/dylight-reg-488-conjugated-avidin-ba1128-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1128_1.jpgFluoro488 Conjugated AvidinMBP was detected in a paraffin-embedded section of rat brain tissues using rabbit anti-MBP Antigen Affinity purified polyclonal antibody (Catalog # PA1050). Biotin conjugated goat anti-rabbit IgG (BA1003) was used to detect the primary antibody. Fluoro488 Conjugated Avidin (Catalog # BA1128) was used for visualization. https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-goat-anti-mouse-igg-secondary-antibody-ba1101-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1101.jpgGoat Anti-Mouse IgG (H+L) Secondary Antibody, FITC ConjugatedGFAP was detected in paraffin-embedded sections of rat brain tissues using mouse anti-GFAP Antigen Affinity purified monoclonal antibody (Catalog # M00213-8). FITC Conjugated Goat Anti-Mouse IgG (BA1101) was used to detect the primary antibody. https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-goat-anti-rabbit-igg-h-l-secondary-antibody-ba1105-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1105-1-IHC-goat-anti-rabbit-igg-secondary-antibody-fitc-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, FITC ConjugatedPKC Iota was detected in paraffin-embedded sections of rat brain tissues using rabbit anti-PKC Iota Antigen Affinity purified polyclonal antibody (Catalog # PB9321) at 1 μg/mL. FITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1105) was used to detect the primary antibody at 20μg/mL. https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-rabbit-anti-rat-igg-h-l-secondary-antibody-ba1108-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Rat IgG (H+L) Secondary Antibody, FITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-rabbit-anti-goat-igg-h-l-secondary-antibody-ba1110-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Goat IgG (H+L) Secondary Antibody, FITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-goat-anti-human-iga-secondary-antibody-ba1112-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGoat Anti-Human IgA Secondary Antibody, FITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-rabbit-anti-human-igg-h-l-secondary-antibody-ba1114-boster.html2026-03-24T05:04:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Human IgG (H+L) Secondary Antibody, FITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-goat-anti-human-igm-secondary-antibody-ba1116-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGoat Anti-Human IgM Secondary Antibody, FITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-protein-a-ba1120-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1120-1_1-IHC-fitc-conjugated-protein-a.jpgFITC Conjugated Protein APCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti-PCK Antigen Affinity purified monoclonal antibody (Catalog # MA1081) at 1 μg/mL. FITC Conjugated Protein A (Catalog # BA1120) was used to detect the primary antibody at 20μg/mL. https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-avidin-ba1125-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1125.jpgFITC Conjugated AvidinHADH was detected in a paraffin-embedded section of human intestinal tissues using rabbit anti-HADH Antigen Affinity purified polyclonal antibody (Catalog # A03650-1). Biotin conjugated goat anti-rabbit IgG (BA1003) was used to detect the primary antibody. DyLight®550 Conjugated Avidin (BA1134) was used for visualization. https://www.bosterbio.com/products/all-secondary-antibodies/fitc-conjugated-mouse-anti-human-igg-secondary-antibody-bm2003-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngMouse Anti-Human IgG (H+L) Secondary Antibody, FITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-goat-anti-mouse-igg-gamma-chain-specific-secondary-antibody-ba1050-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/r/hrp-_ba1050__1.jpgHRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-goat-anti-rabbit-igg-secondary-antibody-ba1054-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/r/hrp-_ba1054_.jpgHRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-rabbit-anti-rat-igg-gamma-chain-specific-secondary-antibody-ba1058-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L)Boster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-rabbit-anti-goat-igg-gamma-chain-specific-secondary-antibody-ba1060-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AffiniPure Rabbit Anti-Goat IgG (H+L)Boster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-goat-anti-human-iga-alpha-chain-specific-secondary-antibody-ba1066-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AffiniPure Goat Anti-human IgABoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-rabbit-anti-human-igg-gamma-chain-specific-secondary-antibody-ba1070-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AffiniPure Rabbit Anti-Human IgG (H+L)Boster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-goat-anti-mouse-igm-u-chain-specific-secondary-antibody-ba1075-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AffiniPure Goat Anti-Mouse IgMBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-goat-anti-human-igm-u-chain-specific-secondary-antibody-ba1077-boster.html2026-03-24T05:04:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AffiniPure Goat Anti-Human IgMBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-protein-a-ba1080-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated Protein ABoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-avidin-ba1081-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AvidinBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-rabbit-anti-avidin-ba1082-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated AffiniPure Rabbit Anti-AvidinBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/hrp-conjugated-streptavidin-ba1088-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHRP Conjugated StreptavidinBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/tritc-conjugated-goat-anti-mouse-igg-secondary-antibody-ba1089-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/a/ba1089-1_2.jpgGoat Anti-Mouse IgG (H+L) Secondary Antibody, TRITC Conjugated IHC analysis of rat heart using anti-Alpha-Smooth Muscle Actin antibody (MA1106).<br> Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 10μg/ml mouse anti-Alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. TRITC Conjugated goat anti-mouse IgG (BA1089) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen. https://www.bosterbio.com/products/all-secondary-antibodies/tritc-conjugated-goat-anti-rabbit-igg-secondary-antibody-ba1090-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1090-1-IHC-goat-anti-rabbit-igg-secondary-antibody-tritc-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, TRITC ConjugatedCpn10 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti-Cpn10 Antigen Affinity purified polyclonal antibody (Catalog # PA1790) at 1 μg/mL. TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1090) was used to detect the primary antibody at 20μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1090-2-IHC-goat-anti-rabbit-igg-secondary-antibody-tritc-conjugate.jpgGoat Anti-Rabbit IgG (H+L) Secondary Antibody, TRITC ConjugatedMCM2 was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-MCM2 Antigen Affinity purified polyclonal antibody (Catalog # PA1650) at 1 μg/mL. TRITC Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1090) was used to detect the primary antibody at 20μg/mL. https://www.bosterbio.com/products/all-secondary-antibodies/tritc-conjugated-rabbit-anti-goat-igg-secondary-antibody-ba1091-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Goat IgG (H+L) Secondary Antibody, TRITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/tritc-conjugated-rabbit-anti-human-igg-secondary-antibody-ba1092-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Human IgG (H+L) Secondary Antibody, TRITC ConjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/tritc-conjugated-avidin-ba1093-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1093-1_1-IHC-tritc-conjugated-avidin.jpgTRITC Conjugated AvidinBeta Catenin was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti-Beta Catenin Antigen Affinity purified polyclonal antibody (Catalog # PA1212) at 1 μg/mL. TRITC Conjugated Avidin (Catalog # BA1093) was used for visualization at 20μg/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/ba1093-2_1-IHC-tritc-conjugated-avidin.jpgTRITC Conjugated AvidinPCK was detected in paraffin-embedded sections of human intestinal cancer tissues using mouse anti-PCK Antigen Affinity purified monoclonal antibody (Catalog # MA1081) at 1 μg/mL. TRITC Conjugated Avidin (Catalog # BA1093) was used for visualization at 20μg/mL. https://www.bosterbio.com/products/all-secondary-antibodies/unconjugated-goat-anti-mouse-igg-secondary-antibody-ba1038-boster.html2026-03-24T05:04:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGoat Anti-Mouse IgG (H+L) Secondary Antibody, UnconjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/unconjugated-goat-anti-rabbit-igg-secondary-antibody-ba1039-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGoat Anti-Rabbit IgG (H+L) Secondary Antibody, UnconjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/unconjugated-rabbit-anti-goat-igg-secondary-antibody-ba1040-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Goat IgG (H+L) Secondary Antibody, UnconjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/unconjugated-rabbit-anti-human-igg-secondary-antibody-ba1041-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Human IgG (H+L) Secondary Antibody, UnconjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/unconjugated-rabbit-anti-rat-igg-secondary-antibody-ba1042-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Rat IgG (H+L) Secondary Antibody, UnconjugatedBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/human-igg-isotype-control-ba1043-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngHuman IgG (H+L) isotype controlBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/goat-igg-isotype-control-ba1044-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngGoat IgG (H+L) isotype controlBoster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/unconjugated-rabbit-igg-ba1045-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit IgG Isotype Control (H+L)Boster Kit Box https://www.bosterbio.com/products/all-secondary-antibodies/mouse-igg-isotype-control-ba1046-boster.html2026-03-24T05:04:22+00:00daily0.9 https://www.bosterbio.com/products/all-secondary-antibodies/unconjugated-rabbit-anti-mouse-igg-secondary-antibody-ba1048-boster.html2026-03-24T05:04:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngRabbit Anti-Mouse IgG (H+L) Secondary Antibody, UnconjugatedBoster Kit Box https://www.bosterbio.com/products/poly-l-lysine-coated-slides-for-ihc-f-and-ihc-p-ar1065-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1065-1.jpgPoly-L-Lysine Coated Slideshttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1065-2.jpgPoly-L-Lysine Coated Slides https://www.bosterbio.com/products/4-paraformaldehyde-pfa-solution-in-pbs-ar1068-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1068_1.jpg4% Paraformaldehyde (PFA) Solution in PBS (with DEPC) https://www.bosterbio.com/products/buffers-in-dry-powder-form/citrate-buffer-ar0024-boster.html2026-03-24T05:04:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0024_2.jpgCitrate Buffer Powderhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/c/i/citrate_buffer_solution.pngCitrate Buffer PowderChemical structure of Sodium Citrate https://www.bosterbio.com/products/dapi-4-6-diamidino-2-phenylindole-dihydrochloride-for-nucleic-acid-staining-ar1176-boster.html2026-03-26T05:19:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1176_1.jpgDAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1177.jpgDAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid staininghttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/d/a/dapi_image.jpgDAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) for nucleic acid stainingNIH-3T3 cells were grown in 96 well plates&#44; fixed with paraformaldehyde and permeabilized. Then incubate with Boster DAPI solution for 5 minutes. https://www.bosterbio.com/products/nuclear-fast-red-ar0008-boster.html2026-03-31T05:01:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0008_1_1.jpgNuclear Fast Red Solutionhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0008-100_2.jpgNuclear Fast Red Solution https://www.bosterbio.com/products/cytoplasmic-and-nuclear-protein-extraction-kit-ar0106-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0106.jpgCytoplasmic and Nuclear Protein Extraction Kithttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0106.pngCytoplasmic and Nuclear Protein Extraction KitNuclear proteins and cytoplasmic proteins were extracted from mouse heart&#44; kidney&#44; lung and liver tissues (100mg) with Boster Cytoplasmic and Nuclear Protein Extraction Kit. BCA protein Assay kit (Product No. AR0146) was used to quantify isolated nuclear and cytoplasmic proteins.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0106_1.pngCytoplasmic and Nuclear Protein Extraction KitNuclear proteins (NE) and cytoplasmic proteins (CE) were extracted from mouse heart&#44; kidney&#44; lung and liver tissues with Boster Cytoplasmic and Nuclear Protein Extraction Kit. Proteins were used to perform WB for GAPDH and PCNA expressed in cytoplasm and in nuclei. https://www.bosterbio.com/products/ripa-lysis-buffer-ar0105-boster.html2026-04-01T05:01:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0105-100_1.jpgRIPA Lysis Buffer https://www.bosterbio.com/products/sds-page-protein-loading-buffer-2x-reducing-ar0131-boster.html2026-03-31T05:01:06+00:00daily0.9 https://www.bosterbio.com/products/sds-page-protein-loading-buffer-5x-reducing-ar1112-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1112-20.jpgSDS-PAGE Protein Loading Buffer 5X (Reducing) https://www.bosterbio.com/products/nitrocellulose-membrane-for-western-blot-transfer-pore-0-45-m-size-9cm-x-10cm-ar0135-04-boster.html2026-03-31T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0135-04_1_1.jpgNitrocellulose Membrane For Western Blot Transfer, Pore 0.45µm, Size 9cm X 10cm https://www.bosterbio.com/products/western-blotting-filter-paper-ar0172-boster.html2026-03-31T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0172_1_1.jpgWestern Blotting Filter Paper, 0.158mm thick, 12.5cm × 12.5cm https://www.bosterbio.com/products/western-blotting-filter-paper-ar0173-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar0173.jpgWestern Blotting Filter Paper, 0.158mm thick, 9cm × 7cm https://www.bosterbio.com/products/broad-spectrum-protease-inhibitor-cocktail-ar1182-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1182_1.jpgBroad Spectrum Protease Inhibitor Cocktail (100x)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1182_1.gifBroad Spectrum Protease Inhibitor Cocktail (100x)Protease activity for extracted protein from mouse tissue while using different protease inhibitor cocktails https://www.bosterbio.com/catalog/product/view/id/21712026-03-24T05:04:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngSample diluent bufferBoster Kit Box https://www.bosterbio.com/catalog/product/view/id/21722026-03-24T05:04:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAntibody diluent bufferBoster Kit Box https://www.bosterbio.com/catalog/product/view/id/21732026-03-31T05:01:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngABC diluent bufferBoster Kit Box https://www.bosterbio.com/products/cell-counting-kit-8-cck-8-ar1160-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1160.jpgCell Counting Kit-8 (CCK-8)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1160.gifCell Counting Kit-8 (CCK-8) Absorption spectrum of WST-8 formazan <br> Figure 1 shows the absorption spectrum of WST-8 formazan. Since the absorbance at 460 nm is proportional to the number of viable cells in the medium&#44; the viable cell number can be determined using the absorbance of a previously prepared calibration curve.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1160_1.gifCell Counting Kit-8 (CCK-8) Cell proliferation assay using CCK-8<br> Culture medium: MEM&#44; 10% FBS <br>Incubation: 37 ℃&#44; 5% CO2&#44; 2 hours <br>Deteactin: 450nmhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1160_1_1.gifCell Counting Kit-8 (CCK-8) Toxicological test of chemicals using CCK-8 <br> Cell line: hela <br>Medium: DMEM&#44; 10% FBS Chemicals: 200 μM Cisplatin (DDP) <br>Incubation: 37°C&#44; 5% CO2&#44; 2 hours https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bid-picoband-trade-antibody-pb9027-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9027-bid-primary-antibodies-wb-testing-1_1.jpgAnti-BID Antibody Picoband&reg; Western blot analysis of Bid using anti-Bid antibody (PB9027). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bid antigen affinity purified polyclonal antibody (Catalog # PB9027) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bid at approximately 22 kDa. The expected band size for Bid is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9027-bid-primary-antibodies-wb-testing-2.jpgAnti-BID Antibody Picoband&reg;Western blot analysis of BID using anti-BID antibody (PB9027).<br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br> Lane 1: huamn OE19 whole cell lysates,<br> Lane 2: human OE19 whole cell lysates,<br> Lane 3: human OE33 whole cell lysates,<br> Lane 4: human OE33 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BID antigen affinity purified monoclonal antibody (PB9027) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BID at approximately ~22 kDa. The expected band size for BID is at ~22 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9027-2-IHC-anti-bid-picoband-antibody.jpgAnti-BID Antibody Picoband&reg; IHC analysis of BID using anti-BID antibody (PB9027).<br> BID was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9027-3-IHC-anti-bid-picoband-antibody.jpgAnti-BID Antibody Picoband&reg; IHC analysis of BID using anti-BID antibody (PB9027).<br> BID was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9027-4-ihc-anti-bid-picoband-antibody.jpgAnti-BID Antibody Picoband&reg; IHC analysis of BID using anti-BID antibody (PB9027).<br> BID was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9027-5-if-anti-bid-picoband-antibody.jpgAnti-BID Antibody Picoband&reg; IHC analysis of BID using anti-BID antibody (PB9027).<br> BID was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-BID Antibody (PB9027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9027-bid-primary-antibodies-fc-testing-6.jpgAnti-BID Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-BID antibody (PB9027). <br>Overlay histogram showing MCF-7 cells stained with PB9027 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BID Antibody (PB9027&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9027-bid-primary-antibodies-if-testing-7.jpgAnti-BID Antibody Picoband&reg; IF analysis of Bid using anti-Bid antibody (PB9027). <br> Bid was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Bid Antibody (PB9027) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-i-309-picoband-trade-antibody-pb9028-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9028-1-WB-anti-ccl1-i-309-antibody.jpgAnti-I-309/CCL1 Antibody Picoband&reg; Western blot analysis of I-309 using anti-I-309 antibody (PB9028).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: U87 Whole Cell Lysate,<br> Lane 2: MCF-7 Whole Cell Lysate,<br> Lane 3: COLO320 Whole Cell Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-I-309 antigen affinity purified polyclonal antibody (Catalog # PB9028) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for I-309 at approximately 11KD. The expected band size for I-309 is at 11KD. https://www.bosterbio.com/picokine-elisa-kits/human-il-17rb-picokine-trade-elisa-kit-ek0785-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0785.pngHuman IL-17RB ELISA Kit PicoKine®Human IL-17RB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-17c-picokine-trade-elisa-kit-ek0789-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0789.jpgHuman IL-17C ELISA Kit PicoKine®Human IL-17C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-17c-picokine-trade-elisa-kit-ek0790-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0790.jpgMouse IL-17C ELISA Kit PicoKine®Mouse IL-17C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-livin-picokine-trade-elisa-kit-ek1348-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1348.pngHuman Livin ELISA Kit PicoKine®Human Livin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-jam-a-picokine-trade-elisa-kit-ek1349-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1349.jpgHuman JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®Human JAM-A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-20-picokine-trade-elisa-kit-ek1350-boster.html2026-03-24T05:04:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1350.pngMouse IL-20/Interleukin-20 ELISA Kit PicoKine®Mouse IL-20 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-epo-picokine-trade-elisa-kit-ek1351-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1351.jpgRat EPO/Erythropoietin ELISA Kit PicoKine®Rat EPO PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-angptl3-picokine-trade-elisa-kit-ek1352-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1352.pngMouse ANGPTL3 ELISA Kit PicoKine®Mouse ANGPTL3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcp3-picoband-trade-antibody-pb9029-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9029-1-WB-anti-ccl7-mcp-3-antibody.jpgAnti-MCP3/CCL7 Antibody Picoband&reg; Western blot analysis of MCP3 using anti-MCP3 antibody (PB9029).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinant Mouse MCP3 Protein 0.5ng.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP3 antigen affinity purified polyclonal antibody (Catalog # PB9029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP3 at approximately 36KD. The expected band size for MCP3 is at 36KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcp4-picoband-trade-antibody-pb9030-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9030-1-WB-anti-ccl13-mcp4-antibody.jpgAnti-MCP4/CCL13 Antibody Picoband&reg; Western blot analysis of MCP4 using anti-MCP4 antibody (PB9030).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinant Human MCP4 Protein 0.5ng.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP4 antigen affinity purified polyclonal antibody (Catalog # PB9030) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP4 at approximately 34KD. The expected band size for MCP4 is at 34KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tarc-picoband-trade-antibody-pb9031-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9031-1-WB-anti-ccl17-tarc-antibody.jpgAnti-TARC/CCL17 Antibody Picoband&reg; Western blot analysis of TARC using anti-TARC antibody (PB9031).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinant Mouse TARC Protein 0.5ng.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARC antigen affinity purified polyclonal antibody (Catalog # PB9031) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TARC at approximately 34KD. The expected band size for TARC is at 34KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cntf-picoband-trade-antibody-pb9032-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9032-1-IHC-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; IHC analysis of CNTF using anti-CNTF antibody (PB9032).<br> CNTF was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CNTF Antibody (PB9032) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9032-2-IHC-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; IHC analysis of CNTF using anti-CNTF antibody (PB9032).<br> CNTF was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CNTF Antibody (PB9032) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9032-3_1.jpgAnti-CNTF Antibody Picoband&reg; Western blot analysis of CNTF using anti-CNTF antibody (PB9032). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody (Catalog # PB9032) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNTF at approximately 29KD. The expected band size for CNTF is at 23KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cofilin-picoband-trade-antibody-pb9033-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-cofilin-primary-antibodies-wb-testing-1.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; Western blot analysis of Cofilin using anti-Cofilin antibody (PB9033). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: monkey COS-7 whole cell lysates,<br> Lane 3: human HEK293 whole cell lysates,<br> Lane 4: human U-87MG whole cell lysates,<br> Lane 5: human HepG2 whole cell lysates,<br> Lane 6: human PC-3 whole cell lysates,<br> Lane 7: human THP-1 whole cell lysates,<br> Lane 8: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin antigen affinity purified polyclonal antibody (Catalog # PB9033) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin at approximately 19 kDa. The expected band size for Cofilin is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-cofilin-primary-antibodies-wb-testing-2.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; Western blot analysis of Cofilin using anti-Cofilin antibody (PB9033). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat spleen tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse spleen tissue lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates,<br> Lane 7: mouse HEPA1-6 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin antigen affinity purified polyclonal antibody (Catalog # PB9033) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin at approximately 19 kDa. The expected band size for Cofilin is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-3_1.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9033).<br> Cofilin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cofilin Antibody (PB9033) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-4_1.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9033).<br> Cofilin was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cofilin Antibody (PB9033) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-cofilin-primary-antibodies-ihc-testing-5.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9033).<br> Cofilin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cofilin Antibody (PB9033) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-cofilin-primary-antibodies-ihc-testing-6.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9033).<br> Cofilin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cofilin Antibody (PB9033) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-9.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Cofilin antibody (PB9033). <br>Overlay histogram showing U20S cells stained with PB9033 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cofilin Antibody (PB9033,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-cofilin-primary-antibodies-ihc-f-testing-7.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9033). <br> Cofilin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cofilin Antibody (PB9033) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9033-cofilin-primary-antibodies-if-testing-8.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IF analysis of Cofilin using anti-Cofilin antibody (PB9033).<br> Cofilin was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Cofilin Antibody (PB9033) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-6-picoband-trade-antibody-pb9034-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9034-jitc-2022-006493f02.jpgAnti-IL6 Antibody Picoband&reg;TA significantly suppressed M2 polarization of macrophages in vitro. (A) Morphological transformation and representative immunofluorescent staining of M1 markers (CD80 and iNOS) and M2 markers (CD206 and Arg1) in M2 macrophages treated with 25 μM TA for 48 hours. (B) The mRNA levels of CD206, Arg1, TNF-α, and iNOS in M2 macrophages were measured by qRT-PCR. n=3. (C) The secretion levels of polyamine were measured by ELISA (n=3). (D, E) Protein expression levels of CD206, iNOS, CD80, Arg1, and IL-6 in M2 macrophages treated with different concentrations of TA were determined by western blot. (F) CD206+ macrophages (gated on CD11b+F4/80+ macrophages) were quantitatively determined by flow cytometry assay. (G) Schematic illustration of coculture system. (H, I) IFN-γ+CD8+ T cells in the coculture system were quantitatively determined by flow cytometry assay. (J) The secretion levels of IFN-γ were measured by ELISA (n=3). (K) Morphological changes and viability of Hep1-6 HCC cells in coculture system with or without TA treatment. Hep1-6 cell viability was evaluated by CCK-8 assays (n=3). (L) T-cell cytotoxicity to tumor cells was evaluated by LDH assay (n=3). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. aPD-1, programmed cell death receptor 1 antibody; Arg1, arginase-1; Ctrl, control; HCC, hepatocellular carcinoma; IFN-γ, interferon gamma; IL, interleukin; LDH, lactate dehydrogenase; MDSC, myeloid-derived suppressor cell; RT-qPCR, real-time quantitative polymerase chain reaction; TA, tadalafil; TAM, tumor-associated macrophage; TNF-α, tumour necrosis factor alpha; FSC, forward scatter.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9950981/&orig_host=www.ncbi.nlm.nih.gov'>36813307</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9034-il6-primary-antibodies-wb-testing-1.jpgAnti-IL6 Antibody Picoband&reg; Western blot analysis of IL6 using anti-IL6 antibody (PB9034). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse J774A.1 whole cell lysates,<br> Lane 2: mouse RAW264.7 whole cell lysates,<br> Lane 3: mouse ANA-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # PB9034) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL6 at approximately 30 kDa. The expected band size for IL6 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9034-il6-primary-antibodies-wb-testing-2.pngAnti-IL6 Antibody Picoband&reg; Western blot analysis of IL6 using anti-IL6 antibody (PB9034). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1-6: mouse PACs whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL6 antigen affinity purified polyclonal antibody (Catalog # PB9034) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. The expected band size for IL6 is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-12-p40-antibody-rp1028-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9035-1-RP1028-WB-anti-il-12-p40-antibody.jpgAnti-IL12 p40/IL12B Antibody Picoband&reg;Anti-IL-12(p40) Picoband antibody&#44; RP1028<br>All lanes: Anti-IL-12(p40)(RP1028) at 0.5ug/ml<br>WB: Recombinant Rat IL-12(p40) Protein 0.5ng<br>Predicted bind size: 20KD<br>Observed bind size: 20KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lif-picoband-trade-antibody-pb9036-boster.html2026-04-01T05:01:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9036-1-IHC-anti-lif-picoband-antibody.jpgAnti-LIF Antibody Picoband&reg; IHC analysis of LIF using anti-LIF antibody (PB9036).<br> LIF was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIF Antibody (PB9036) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9036-2-IHC-anti-lif-picoband-antibody.jpgAnti-LIF Antibody Picoband&reg; IHC analysis of LIF using anti-LIF antibody (PB9036).<br> LIF was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LIF Antibody (PB9036) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9036-lif-primary-antibodies-wb-testing-3.jpgAnti-LIF Antibody Picoband&reg; Western blot analysis of LIF using anti-LIF antibody (PB9036). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human placenta tissue lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIF antigen affinity purified polyclonal antibody (Catalog # PB9036) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIF at approximately 36 kDa. The expected band size for LIF is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp7-picoband-trade-antibody-pb9037-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9037-1-IHC-anti-mmp-7-antibody.jpgAnti-MMP7 Antibody Picoband&reg; IHC analysis of MMP7 using anti-MMP7 antibody (PB9037).<br> MMP7 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP7 Antibody (PB9037) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9037-2-IHC-anti-mmp-7-antibody.jpgAnti-MMP7 Antibody Picoband&reg; IHC analysis of MMP7 using anti-MMP7 antibody (PB9037).<br> MMP7 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP7 Antibody (PB9037) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9037-3-WB-anti-mmp-7-antibody.jpgAnti-MMP7 Antibody Picoband&reg; Western blot analysis of MMP7 using anti-MMP7 antibody (PB9037).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: A549 Whole Cell Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP7 antigen affinity purified polyclonal antibody (Catalog # PB9037) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP7 at approximately 30KD. The expected band size for MMP7 is at 30KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp7-picoband-trade-antibody-pb9038-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9038-1-IHC-anti-mmp-7-antibody.jpgAnti-MMP7 Antibody Picoband&reg; IHC analysis of MMP7 using anti-MMP7 antibody (PB9038).<br> MMP7 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP7 Antibody (PB9038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9038-2-IHC-anti-mmp-7-antibody.jpgAnti-MMP7 Antibody Picoband&reg; IHC analysis of MMP7 using anti-MMP7 antibody (PB9038).<br> MMP7 was detected in paraffin-embedded section of rat intestines tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP7 Antibody (PB9038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9038-3-WB-anti-mmp-7-antibody.jpgAnti-MMP7 Antibody Picoband&reg; Western blot analysis of MMP7 using anti-MMP7 antibody (PB9038).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: Rat Brain Tissue Lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP7 antigen affinity purified polyclonal antibody (Catalog # PB9038) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP7 at approximately 30KD. The expected band size for MMP7 is at 30KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acth-picoband-trade-antibody-pb9039-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9039-1-IHC-anti-acth-picoband-antibody.jpgAnti-ACTH/POMC Antibody IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9039-2-IHC-anti-acth-picoband-antibody.jpgAnti-ACTH/POMC Antibody IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9039-3-IHC-anti-acth-picoband-antibody.jpgAnti-ACTH/POMC Antibody IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ara9-picoband-trade-antibody-pb9042-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9042-ara9-primary-antibodies-wb-testing-1.jpgAnti-ARA9/AIP Antibody Picoband&reg; Western blot analysis of ARA9 using anti-ARA9 antibody (PB9042). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human COLO320 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human SW620 whole cell lysates,<br> Lane 5: human U87 whole cell lysates,<br> Lane 6: human MDA-MB-453 whole cell lysates,<br> Lane 7: human Jurkat whole cell lysates,<br> Lane 8: human CACO-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARA9 antigen affinity purified polyclonal antibody (Catalog # PB9042) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARA9 at approximately 38 kDa. The expected band size for ARA9 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9042-2.jpgAnti-ARA9/AIP Antibody Picoband&reg; IF analysis of ARA9 using anti-ARA9 antibody (PB9042). <br> ARA9 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ARA9 Antibody (PB9042) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9042-3.jpgAnti-ARA9/AIP Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ARA9 antibody (PB9042).<br>Overlay histogram showing A431 cells stained with PB9042 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARA9 Antibody (PB9042,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akt2-picoband-trade-antibody-pb9043-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9043-akt2-primary-antibodies-wb-testing-1.jpgAnti-AKT2 Antibody Picoband&reg; Western blot analysis of AKT2 using anti-AKT2 antibody (PB9043). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SW620 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT2 antigen affinity purified polyclonal antibody (Catalog # PB9043) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT2 at approximately 56 kDa. The expected band size for AKT2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9043-2-IHC-anti-akt2-picoband-antibody.jpgAnti-AKT2 Antibody Picoband&reg; IHC analysis of AKT2 using anti-AKT2 antibody (PB9043).<br> AKT2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9043-3-ihc-anti-akt2-picoband-antibody.jpgAnti-AKT2 Antibody Picoband&reg; IHC analysis of AKT2 using anti-AKT2 antibody (PB9043).<br> AKT2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9043-4.jpgAnti-AKT2 Antibody Picoband&reg; IHC analysis of AKT2 using anti-AKT2 antibody (PB9043).<br> AKT2 was detected in frozen section of rat brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKT2 Antibody (PB9043) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-v-picoband-trade-antibody-pb9044-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9044-anxa5-primary-antibodies-wb-testing-1.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg; Western blot analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (PB9044). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat heart tissue lysates,<br> Lane 6: mouse heart tissue lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin V/ANXA5 antigen affinity purified polyclonal antibody (PB9044) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Annexin V/ANXA5 at approximately 36 kDa. The expected band size for Annexin V/ANXA5 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9044-anxa5-primary-antibodies-ihc-testing-2.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg; IHC analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (PB9044). <br> Annexin V/ANXA5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin V/ANXA5 Antibody (PB9044) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9044-anxa5-primary-antibodies-ihc-testing-3.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg; IHC analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (PB9044). <br> Annexin V/ANXA5 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin V/ANXA5 Antibody (PB9044) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9044-anxa5-primary-antibodies-ihc-testing-4.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg; IHC analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (PB9044). <br> Annexin V/ANXA5 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Annexin V/ANXA5 Antibody (PB9044) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9044-anxa5-primary-antibodies-if-testing-5.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg; IF analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (PB9044). <br> Annexin V/ANXA5 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Annexin V/ANXA5 Antibody (PB9044) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9044-anxa5-primary-antibodies-fcm-testing-6.jpgAnti-Annexin V/ANXA5 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-Annexin V/ANXA5 antibody (PB9044). <br> Overlay histogram showing 293T cells stained with PB9044 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Annexin V/ANXA5 Antibody (PB9044, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calbindin-picoband-trade-antibody-pb9045-boster.html2026-03-24T05:04:31+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9045-calbindin-primary-antibodies-wb-testing-1.jpgAnti-Calbindin/CALB1 Antibody Picoband&reg; Western blot analysis of Calbindin using anti-Calbindin antibody (PB9045). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse kidney tissue lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody (Catalog # PB9045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calbindin at approximately 26 kDa. The expected band size for Calbindin is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9045-calbindin-primary-antibodies-ihc-testing-2.jpgAnti-Calbindin/CALB1 Antibody Picoband&reg; IHC analysis of Calbindin using anti-Calbindin antibody (PB9045). <br> Calbindin was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calbindin Antibody (PB9045) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9045-calb1-primary-antibodies-ihc-testing-4.jpgAnti-Calbindin/CALB1 Antibody Picoband&reg;IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045). <br>Calbindin/CALB1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calbindin/CALB1 Antibody (PB9045) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9045-calb1-primary-antibodies-ihc-testing-5.jpgAnti-Calbindin/CALB1 Antibody Picoband&reg;IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045). <br>Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calbindin/CALB1 Antibody (PB9045) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9045-calb1-primary-antibodies-ihc-testing-6.jpgAnti-Calbindin/CALB1 Antibody Picoband&reg;IHC analysis of Calbindin/CALB1 using anti-Calbindin/CALB1 antibody (PB9045). <br>Calbindin/CALB1 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calbindin/CALB1 Antibody (PB9045) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9045-calbindin-primary-antibodies-ihc-testing-3.jpgAnti-Calbindin/CALB1 Antibody Picoband&reg; IHC analysis of Calbindin using anti-Calbindin antibody (PB9045). <br> Calbindin was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Calbindin Antibody (PB9045) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9045-calbindin-primary-antibodies-ip-testing-4.jpgAnti-Calbindin/CALB1 Antibody Picoband&reg; Immunoprecipitating (IP) Calbindin in K562 whole cell lysate.<br> Western blot analysis of Calbindin using anti-Calbindin antibody (PB9045); <br> Lane 1: K562 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Calbindin antibody in K562 whole cell lysate;<br> Lane 3: anti-Calbindin antibody (2μg) + K562 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody (PB9045) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Calbindin at approximately 26 kDa. The expected band size for Calbindin is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-b-picoband-trade-antibody-pb9046-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9046-ctsb-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin B/CTSB Antibody Picoband&reg; Western blot analysis of Cathepsin B using anti-Cathepsin B antibody (PB9046). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 5 ng of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin B antigen affinity purified polyclonal antibody (Catalog # PB9046) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin B at approximately 33 kDa. The expected band size for Cathepsin B is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-d-picoband-trade-antibody-pb9047-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9047-1-IHC-anti-cathepsin-d-picoband-antibody.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9047). <br> Cathepsin D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cathepsin D Antibody (PB9047) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9047-2-WB-anti-cathepsin-d-picoband-antibody.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (PB9047). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human Cathepsin D protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody (Catalog # PB9047) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 40 kDa. The expected band size for Cathepsin D is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-d-picoband-trade-antibody-pb9048-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9048-1-IHC-anti-cathepsin-d-picoband-antibody.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048).<br> Cathepsin D was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9048) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9048-2-IHC-anti-cathepsin-d-picoband-antibody.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048).<br> Cathepsin D was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9048) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9048-3-WB-anti-cathepsin-d-picoband-antibody.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Mouse Liver Tissue Lysate<br> Lane 2: Mouse Brain Tissue Lysate<br> Lane 3: Mouse Thymus Tissue Lysate<br> Lane 4: NEURO Whole Cell Lysate<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody (Catalog # PB9048) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 45KD. The expected band size for Cathepsin D is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9048-4.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048). <br> Cathepsin D was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9048) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9048-ctsd-primary-antibodies-fc-testing-1.pngAnti-Cathepsin D/CTSD Antibody Picoband&reg;Flow Cytometry analysis of mouse liver single-cell suspension using anti-Cathepsin D/CTSD antibody (PB9048).<br> Overlay histogram showing mouse liver single-cell suspension stained with Cathepsin D/CTSD (Blue line). mouse liver single-cell suspension had been fixed and permeabilized. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cathepsin D/CTSD Antibody (PB9048, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-g-picoband-trade-antibody-pb9049-boster.html2026-03-25T05:21:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9049-ctsg-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin G/CTSG Antibody Picoband&reg;Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (PB9049). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (PB9049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 29 kDa. The expected band size for Cathepsin G is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9049-ctsg-primary-antibodies-wb-testing-2.jpgAnti-Cathepsin G/CTSG Antibody Picoband&reg;Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (PB9049). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (PB9049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 29 kDa. The expected band size for Cathepsin G is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9049-ctsg-primary-antibodies-ihc-testing-1.jpgAnti-Cathepsin G/CTSG Antibody Picoband&reg;IHC analysis of Cathepsin G using anti-Cathepsin G antibody (PB9049). <br>Cathepsin G was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin G Antibody (PB9049) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd20-picoband-trade-antibody-pb9050-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9050-2-IHC-anti-cd20-picoband-antibody.jpgAnti-CD20/MS4A1 Antibody Picoband&reg; IHC analysis of CD20 using anti-CD20 antibody (PB9050).<br>CD20 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD20 Antibody (PB9050) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9050-ms4a1-primary-antibodies-wb-testing-3.jpgAnti-CD20/MS4A1 Antibody Picoband&reg; Western blot analysis of CD20 using anti-CD20 antibody (PB9050). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD20 antigen affinity purified polyclonal antibody (Catalog # PB9050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD20 at approximately 35 kDa. The expected band size for CD20 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9050-3-ihc-anti-cd20-picoband-antibody.jpgAnti-CD20/MS4A1 Antibody Picoband&reg; IHC analysis of CD20 using anti-CD20 antibody (PB9050).<br>CD20 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD20 Antibody (PB9050) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9050-cd20-primary-antibodies-if-testing-4.jpgAnti-CD20/MS4A1 Antibody Picoband&reg; IF analysis of CD20 using anti-CD20 antibody (PB9050) <br> CD20 was detected in paraffin-embedded section of human B lymphocytic tumor tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD20 Antibody (PB9050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9050-5.pngAnti-CD20/MS4A1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-CD20 antibody (PB9050). <br> Overlay histogram showing A431 cells stained with PB9050 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD20 Antibody (PB9050&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd23-picoband-trade-antibody-pb9051-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-cd23-primary-antibodies-wb-testing-1.jpgAnti-CD23/FCER2 Antibody Picoband&reg; Western blot analysis of CD23 using anti-CD23 antibody (PB9051). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat PC-12 whole cell lysates,<br> Lane 4: mouse spleen tissue lysates,<br> Lane 5: mouse thymus tissue lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD23 antigen affinity purified polyclonal antibody (Catalog # PB9051) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD23 at approximately 49 kDa. The expected band size for CD23 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-fimmu-14-1217492-g004.jpgAnti-CD23/FCER2 Antibody Picoband&reg;FB cells in pSS model mice. (A) F (CD23 + CD21 − ) and MZ (CD23 − CD21 + ) B cells among the CD19 + cells identified in the spleen, the salivary glands, and the lungs of 10-week-old control and pSS model. Representative results are shown. (B) Proportions and numbers of follicular B cells in the spleen of 10-week-old control mice and pSS model mice. Data are presented as mean ± SD of four to five mice per group. (C) Proportions and numbers of follicular B cells in the salivary gland tissues of 12-week-old control and SS model mice. Data are presented as mean ± SD of four mice per group. (D) Population of follicular B cells in the lungs of 8- and 16-week-old control mice and SS model mice. Data are presented as mean ± SD of three to eight mice per group. * p < 0.05, ** p < 0.01. (E) CD23 + cells were detected through immunohistochemical analysis by using lung sections of 8-week-old control and SS model mice. Scale bar: 100 μm. (F) B220 + B cells and CD23 + cells were detected through immunofluorescence analysis by using lung sections obtained from 8-week-old control and pSS model mice. Nuclei were stained with DAPI. Scale bar: 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10354287/&orig_host=www.ncbi.nlm.nih.gov'>37475871</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-fimmu-14-1217492-g005.jpgAnti-CD23/FCER2 Antibody Picoband&reg;CD23 + B-cell differentiation via IL-4 in the lungs of the pSS model mice. (A) Il4 mRNA expressions were analyzed through qRT-PCR, by using spleen and lung tissues of 8-week-old control and SS model mice. Data are presented as mean ± SD of four mice per group. * p < 0.05. (B) Gata3 (upper panel) and Tbx21 (lower panel) mRNA expressions were analyzed through qRT-PCR, by using spleen, salivary gland, and lung tissues of 8-week-old control and SS model mice. Data are presented as mean ± SD of three to four mice per group. * p < 0.05. (C) CD19 + B cells isolated from the lungs of control and SS model mice were stimulated in vitro with an anti-CD40 mAb (5 µg/ml) and recombinant IL-4 (100 ng/ml) for 7 days. The relative cell number of CD23 + B cells to the unstimulated cells was evaluated. Data are presented as mean ± SD of triplicates per group. * p < 0.05, ** p <0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10354287/&orig_host=www.ncbi.nlm.nih.gov'>37475871</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-fimmu-14-1217492-g006.jpgAnti-CD23/FCER2 Antibody Picoband&reg;CD23 + FB cell differentiation within the lungs of anti-CD4 mAb-treated pSS model mice. (A) Anti-CD4 mAb was intraperitoneally administered to pSS model mice between their sixth to eighth week of their lives. We assessed the proportions of CD4 + T cells in the spleen and of CD4 + T and CD19 + B cells in the lungs of isotype control mAb-treated and of anti-CD4 mAb-treated pSS model mice. Data are presented as mean ± SD of seven mice per group. * p < 0.05. (B) Number of CD4 + T cells in the spleen and of CD4 + T and CD19 + B cells in the lungs of isotype control mAb- and of anti-CD4 mAb-treated pSS model mice. Data are presented as mean ± SD of seven mice per group. ** p < 0.01. (C) Pulmonary lesions in anti-CD4 mAb-treated pSS model mice were histologically evaluated. Representative images of HE-stained lung tissues sections of isotype control mAb-treated and anti-CD4 mAb-treated pSS model mice. Scale bar: 100 μm. (D) The number of foci in the pulmonary lesions was counted by using HE-stained sections. Data are presented as mean ± SD of seven mice per group. (E) CD23 + cells in the pulmonary lesions were evaluated immunohistochemically. Representative images are shown for each group. Scale bar: 100 μm. (F) CD23 + CD19 + FB cells and CD23 − CD19 + B cells were evaluated through flow cytometric analysis by using lung tissues. Representative results are shown for each group. (G) The proportions of CD23 + CD19 + FB cells and of CD23 − CD19 + B cells were analyzed through flow cytometry. Data are presented as mean ± SD of seven mice per group. * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10354287/&orig_host=www.ncbi.nlm.nih.gov'>37475871</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-fimmu-14-1217492-g007.jpgAnti-CD23/FCER2 Antibody Picoband&reg;Preventive effect of the anti-CD4 mAb in the pulmonary lesions of pSS model mice. (A) Anti-CD4 mAb was intraperitoneally administered to pSS model mice between their fourth to sixth week of their lives. Pulmonary lesions in anti-CD4 mAb-treated pSS model mice were histologically evaluated. Representative images of HE-stained lung tissues sections of isotype control mAb-treated and anti-CD4 mAb-treated pSS model mice. Scale bar: 100 μm. (B) The area of foci in the pulmonary lesions was measured by using HE-stained sections. Data are presented as mean ± SD of seven to eight mice per group. ** p <0.01. (C) We assessed the proportions of CD4 + T cells in the spleen and lung of isotype control mAb-treated and of anti-CD4 mAb-treated pSS model mice. Data are presented as mean ± SD of seven to eight mice per group. ** p < 0.01. (D) Number of CD4 + T, CD19 + B, and CD19 + CD23 + FB cells in the lung of isotype control mAb-treated and of anti-CD4 mAb-treated pSS model mice. Data are presented as mean ± SD of seven to eight mice per group. ** p <0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10354287/&orig_host=www.ncbi.nlm.nih.gov'>37475871</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9051-2-IHC-anti-cd23-fcer2-antibody.jpgAnti-CD23/FCER2 Antibody Picoband&reg; IHC analysis of CD23 using anti-CD23 antibody (PB9051).<br> CD23 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD23 Antibody (PB9051) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9051-3-IHC-anti-cd23-fcer2-antibody.jpgAnti-CD23/FCER2 Antibody Picoband&reg; IHC analysis of CD23 using anti-CD23 antibody (PB9051).<br> CD23 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD23 Antibody (PB9051) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-4-ihc-anti-cd23-fcer2-antibody.jpgAnti-CD23/FCER2 Antibody Picoband&reg; IHC analysis of CD23 using anti-CD23 antibody (PB9051).<br> CD23 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD23 Antibody (PB9051) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-5.jpgAnti-CD23/FCER2 Antibody Picoband&reg; IHC analysis of CD23 using anti-CD23 antibody (PB9051).<br> CD23 was detected in frozen section of mouse spleen tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD23 Antibody (PB9051) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-6.jpgAnti-CD23/FCER2 Antibody Picoband&reg; IF analysis of CD23 using anti-CD23 antibody (PB9051) <br> CD23 was detected in paraffin-embedded section of mouse lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD23 Antibody (PB9051) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-8.pngAnti-CD23/FCER2 Antibody Picoband&reg; Flow Cytometry analysis of mouse PBMC cells using anti-CD23 antibody (PB9051).<br> Overlay histogram showing mouse PBMC cells stained with PB9051 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD23 Antibody PB9051&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-7.jpgAnti-CD23/FCER2 Antibody Picoband&reg; IF analysis of CD23 using anti-CD23 antibody (PB9051) <br> CD23 was detected in paraffin-embedded section of rat lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD23 Antibody (PB9051) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-9.pngAnti-CD23/FCER2 Antibody Picoband&reg; Flow Cytometry analysis of mouse BMC cells using anti-CD23 antibody (PB9051). <br> Overlay histogram showing mouse BMC cells stained with PB9051 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD23 Antibody (PB9051&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9051-cd23-primary-antibodies-elisa-testing-10.jpgAnti-CD23/FCER2 Antibody Picoband&reg; Sandwich ELISA - Recombinant mouse CD23/FCER2 protein standard curve.<br> Use in combination with reagents from Mouse CD23/FCER2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0924). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd30-antibody-rp1029-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1029-cd30-primary-antibodies-wb-testing-1.jpgAnti-CD30/TNFRSF8 Antibody Picoband&reg;Western blot analysis of CD30 using anti-CD30 antibody (RP1029). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD30 antigen affinity purified polyclonal antibody (RP1029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD30 at approximately 110 kDa. The expected band size for CD30 is at 64 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd34-picoband-trade-antibody-pb9053-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-wb-testing-1.jpgAnti-CD34 Antibody Picoband&reg; Western blot analysis of CD34 using anti-CD34 antibody (PB9053). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD34 antigen affinity purified polyclonal antibody (Catalog # PB9053) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD34 at approximately 105KD. The expected band size for CD34 is at 105KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-if-testing-10.jpgAnti-CD34 Antibody Picoband&reg; IF analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-ihc-testing-2.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-ihc-testing-3.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-ihc-testing-4.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-ihc-testing-5.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-ihc-testing-6.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-ihc-testing-7.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-ihc-testing-8.jpgAnti-CD34 Antibody Picoband&reg; IHC analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9053-cd34-primary-antibodies-if-testing-9.jpgAnti-CD34 Antibody Picoband&reg; IF analysis of CD34 using anti-CD34 antibody (PB9053). <br> CD34 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-CD34 Antibody (PB9053) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cma1-picoband-trade-antibody-pb9055-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9055-1-IHC-anti-cma1-chymase-picoband-antibody.jpgAnti-Mast Cell Chymase/CMA1 Antibody Picoband&reg; IHC analysis of CMA1 using anti-CMA1 antibody (PB9055). <br> CMA1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CMA1 Antibody (PB9055) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9055-2_2.jpgAnti-Mast Cell Chymase/CMA1 Antibody Picoband&reg; Western blot analysis of CMA1 using anti-CMA1 antibody (PB9055). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates&#44; <br> Lane 2: human HepG2 whole cell lysates&#44; <br> Lane 3: rat liver tissue lysates&#44; <br> Lane 4: mouse HEPA1-6 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CMA1 antigen affinity purified polyclonal antibody (Catalog # PB9055) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CMA1 at approximately 35KD. The expected band size for CMA1 is at 27KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myeloperoxidase-picoband-trade-antibody-pb9057-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-ajtr0012-5131-f5.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Hypoxia pretreatment can improve the recovery efficiency elicited by USCs in a chronic liver fibrosis model. A. Evaluation of liver index and the serum level of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Exogenous cells were visualized using fluorescent imaging. B. Representative H&E and Masson staining images. C. Representative images of immunohistochemistry for α-SMA, MPO, and 8-OHdG. D. Statistical quantification of Masson staining and immunohistochemistry. *P<0.05, **P<0.01, ***P<0.001, one-way ANOVA. Scale bar =200 μm. (α-SMA - α-smooth muscle actin, MPO - myeloperoxidase, 8-OHdG - 8-hydroxy-2’-deoxyguanosine).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7540109/&orig_host=www.ncbi.nlm.nih.gov'>33042410</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-41598_2017_851_fig3_html.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg;Effects of NMN on microglia activation and neutrophil infiltration in cICH model. ( A ) Representative images and quantitative analysis of Iba-1 (microglia marker) immunohistochemistry staining at 24 hours post cICH. ** P < 0.01, n = 5 per group. ( B ) Representative images and quantitative analysis of MPO-1 (neutrophil marker) immunohistochemistry staining at 24 hours post cICH. ** P < 0.01, n = 5 per group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00851-z'>28386082</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-wb-testing-1_1.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; Western blot analysis of MPO using anti-MPO antibody (PB9057). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HL-60 whole cell lysates, <br> Lane 2: human A431 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPO antigen affinity purified polyclonal antibody (Catalog # PB9057) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPO at approximately 60-65kDa, 80-90 kDa. The expected band size for MPO is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-ihc-testing-2.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody (PB9057) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-fcm-testing-5.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-MPO antibody (PB9057). <br>Overlay histogram showing HL-60 cells stained with PB9057 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody (PB9057, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-ihc-testing-3.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody (PB9057) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-ihc-testing-6.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-MPO Antibody (PB9057) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-ihc-testing-4.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody (PB9057) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-ihc-testing-7.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-MPO Antibody (PB9057) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-ihc-testing-8.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IHC analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-MPO Antibody (PB9057) overnight at 4°C. HRP Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-if-testing-9.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IF analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-MPO Antibody (PB9057) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9057-mpo-primary-antibodies-if-testing-10.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; IF analysis of MPO using anti-MPO antibody (PB9057). <br> MPO was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-MPO Antibody (PB9057) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/c/a/casestudy_pb9057.jpgAnti-Myeloperoxidase/MPO Antibody Picoband&reg; https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd10-picoband-trade-antibody-pb9058-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9058-mme-primary-antibodies-fcm-testing-5_1.pngAnti-CD10/MME Antibody Picoband&reg; Flow Cytometry analysis of Daudi cells using anti-CD10/MME antibody (PB9058). <br>Overlay histogram showing Daudi cells stained with PB9058 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD10/MME Antibody (PB9058, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9058-mme-primary-antibodies-wb-testing-1.jpgAnti-CD10/MME Antibody Picoband&reg; Western blot analysis of CD10/MME using anti-CD10/MME antibody (PB9058). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates, <br> Lane 2: human U-87MG whole cell lysates, <br> Lane 3: human placenta tissue lysates, <br> Lane 4: rat kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD10/MME antigen affinity purified polyclonal antibody (Catalog # PB9058) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD10/MME at approximately 100 kDa. The expected band size for CD10/MME is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9058-mme-primary-antibodies-ihc-testing-2.jpgAnti-CD10/MME Antibody Picoband&reg; IHC analysis of CD10/MME using anti-CD10/MME antibody (PB9058). <br> CD10/MME was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD10/MME Antibody (PB9058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9058-mme-primary-antibodies-ihc-testing-3.jpgAnti-CD10/MME Antibody Picoband&reg; IHC analysis of CD10/MME using anti-CD10/MME antibody (PB9058). <br> CD10/MME was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD10/MME Antibody (PB9058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9058-mme-primary-antibodies-ihc-testing-4.jpgAnti-CD10/MME Antibody Picoband&reg; IHC analysis of CD10/MME using anti-CD10/MME antibody (PB9058). <br> CD10/MME was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD10/MME Antibody (PB9058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-src-picoband-trade-antibody-pb9059-boster.html2026-03-24T05:04:32+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9059-oncotarget-06-3123-g005.jpgAnti-Src Antibody Picoband&reg;Slit2/Robo1 signaling activates the Src-mediated inhibition of E-cadherin. IHC analysis of the expression of total Src and pSrc (Tyr416) in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (A). Inactivation of Slit2/Robo1 significantly reduced the expression of pSrc (Tyr 416) in SW620 and SW480 cells, and activation of Slit2/Robo1 signaling through overexpressing Slit2 or Robo1 expression promotes pSrc (Tyr 416) expression in HCT-116 cells (B). IHC analysis of the expression of E-cadherin in the tumor tissues of the Apc Min/+ and Apc Min/+ ; Slit2 mice (C). Inactivation of Src signaling significantly enhances the expression of E-cadherin but inhibits the expression of β-cateninin in SW620 and SW480 cells (D). Src inactivation in SW620 and SW480 cells also led to a reduced nuclear translocation of β-catenin (E). Inhibition of E-cadherin expression through siRNA technique could promote the expression of β-cateninin and vimentin in SW620 and SW480 cells (F). IHC analysis of the expression of vimentin in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (G). The data in IHC staining are representative of 11 mice per group (All mice were 24-week-old). The results of IHC were determined using IPP software, and the data were expressed as the mean ±S.D. *: P < 0.05, **: P < 0.01. Scale bars: 20 μm (A, C and G) and 25 μm (E).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4413642/&orig_host=www.ncbi.nlm.nih.gov'>25605242</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9059-src-primary-antibodies-wb-testing-1_1.jpgAnti-Src Antibody Picoband&reg; Western blot analysis of Src using anti-Src antibody (PB9059). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human RT4 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human SH-SY5Y whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Src antigen affinity purified polyclonal antibody (Catalog # PB9059) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Src at approximately 60 kDa. The expected band size for Src is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9059-src-primary-antibodies-fcm-testing-4.jpgAnti-Src Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Src antibody (PB9059). <br>Overlay histogram showing HepG2 cells stained with PB9059 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Src Antibody (PB9059, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9059-src-primary-antibodies-ihc-testing-2.jpgAnti-Src Antibody Picoband&reg; IHC analysis of Src using anti-Src antibody (PB9059). <br> Src was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Src Antibody (PB9059) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9059-src-primary-antibodies-ihc-testing-3.jpgAnti-Src Antibody Picoband&reg; IHC analysis of Src using anti-Src antibody (PB9059). <br> Src was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Src Antibody (PB9059) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vwf-picoband-trade-antibody-pb9062-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9062-1-IHC-anti-vwf-von-willebrand-factor-picoband-antibody.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg; IHC analysis of VWF using anti-VWF antibody (PB9062).<br>VWF was detected in paraffin-embedded section of Human Lung Cancer Tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VWF Antibody (PB9062) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9062-41598_2023_49339_fig9_html.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;H&E, Masson’s trichrome stain, and IHC examinations of the retrieved tissue and the tendon. ( A ) H&E stain; ( B ) Masson’s trichrome stain; ( C ) VEGF; ( D ) BMP-2; ( E ) TGF-β; ( F ) vWF; ( G ) collagen I; ( H ) collagen III. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-023-49339-z'>38081952</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9062-fphar-13-1005014-g005.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;Effects of TMP on the cytoskeleton in vitro . (A) VWF staining was used to identify endothelial cells (scale bar = 50 μm). (B) Transwell chamber FITC albumin was used to determine the effect of TMP (15 ng/ml) on HUVEC after 12 h of LPS (200 ng/ml) exposure. (C) Immunofluorescence assay for the cytoskeleton (scale bar = 50 μm). (D) Transendothelial electrical resistance (TEER) in TMP-treated, untreated, and control HUVECs. * p < 0.05, ** p < 0.01, n = 3 per group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9859661/&orig_host=www.ncbi.nlm.nih.gov'>36686718</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9062-ajtr0009-4492-f1.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;Cell identification and cell viability test. HUVECs were isolated by collagenase II digestion (A) and identified by vWF factors (× 100 magnification) (B). (C) Cell viability was detected with CCK-8. Different concentrations of FGF21 were added to the medium and absorbance was observed after 12 h. Values are the mean ± SD; n = 5 in each group. Different letters indicate differences between the treatment groups, P < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5666058/&orig_host=www.ncbi.nlm.nih.gov'>29118911</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9062-2-WB-anti-vwf-von-willebrand-factor-picoband-antibody.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg; Western blot analysis of VWF using anti-VWF antibody (PB9062). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.<br> Lane 1: HT1080 Whole Cell Lysate.<br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VWF antigen affinity purified polyclonal antibody (Catalog # PB9062) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VWF at approximately 309KD. The expected band size for VWF is at 309KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9062-vwf-primary-antibodies-ihc-testing-3.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg; IHC analysis of VWF using anti-VWF antibody (PB9062).<br> VWF was detected in frozen section of human placenta tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VWF Antibody (PB9062) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9062-4.pngAnti-Von Willebrand Factor/VWF Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-VWF antibody (PB9062). <br> Overlay histogram showing A431 cells stained with PB9062 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VWF Antibody (PB9062&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt5a-picoband-trade-antibody-pb9063-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9063-2-IHC-anti-wnt5a-picoband-antibody.jpgAnti-Wnt5a Antibody Picoband&reg; IHC analysis of Wnt5a using anti-Wnt5a antibody (PB9063). <br> Wnt5a was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Wnt5a Antibody (PB9063) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9063-1_1.jpgAnti-Wnt5a Antibody Picoband&reg; Western blot analysis of Wnt5a using anti-Wnt5a antibody (PB9063). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysate&#44; <br> Lane 2: human PC-3 whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt5a antigen affinity purified polyclonal antibody (Catalog # PB9063) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt5a at approximately 45KD. The expected band size for Wnt5a is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9063-wnt5a-primary-antibodies-ihc-testing-3.jpgAnti-Wnt5a Antibody Picoband&reg; IHC analysis of Wnt5a using anti-Wnt5a antibody (PB9063). <br> Wnt5a was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Wnt5a Antibody (PB9063) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccl4-mip-1-beta-picoband-trade-antibody-pb9064-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9064-ccl4-primary-antibodies-wb-testing-1.jpgAnti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband&reg; Western blot analysis of CCL4 using anti-CCL4 antibody (PB9064). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant rat CCL4 protein 10 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL4 antigen affinity purified polyclonal antibody (Catalog # PB9064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL4 at approximately 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9064-ccl4-primary-antibodies-ihc-testing-2.jpgAnti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband&reg; IHC analysis of CCL4 using anti-CCL4 antibody (PB9064). <br> CCL4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCL4 Antibody (PB9064) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9064-ccl4-primary-antibodies-ihc-testing-3.jpgAnti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband&reg; IHC analysis of CCL4 using anti-CCL4 antibody (PB9064). <br> CCL4 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCL4 Antibody (PB9064) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eotaxin-picoband-trade-antibody-pb9065-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9065-1-WB-anti-eotaxin-picoband-antibody.jpgAnti-Eotaxin/CCL11 Antibody Picoband&reg; Western blot analysis of Eotaxin using anti-Eotaxin antibody (PB9065). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse lung tissue lysates, <br> Lane 2: mouse intestines tissue lysates, <br> Lane 3: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin antigen affinity purified polyclonal antibody (Catalog # PB9065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eotaxin at approximately 11 kDa. The expected band size for Eotaxin is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eotaxin-3-picoband-trade-antibody-pb9066-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9066-1-IHC-anti-eotaxin-3-picoband-antibody.jpgAnti-Eotaxin 3/CCL26 Antibody Picoband&reg; IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066). <br> Eotaxin 3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9066-2-IHC-anti-eotaxin-3-picoband-antibody.jpgAnti-Eotaxin 3/CCL26 Antibody Picoband&reg; IHC analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066). <br> Eotaxin 3 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Eotaxin 3 Antibody (PB9066) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9066-12672_2025_2280_fig1_html.pngAnti-Eotaxin 3/CCL26 Antibody Picoband&reg;Flowchart of the study. A Data acquisition and patient recruitment; B workflow for the construction and evaluation of the radiomics model; C Development of rad-score based prognostic nomograms; D Representative pathological, immunohistochemical, and CT images of the regions of interest showing the difference in CCL26 expression between tumor and non—tumor tissues. TCGA-LIHC The Cancer Genome Atlas Hepatocellular Carcinoma, TCIA The Cancer Imaging Archive, GTEx Genotype-Tissue Expression Project, VOI volume of interest, ICC intraclass correlation coefficient, ROC receiver operating characteristic <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s12672-025-02280-1'>40205283</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9066-12672_2025_2280_fig2_html.pngAnti-Eotaxin 3/CCL26 Antibody Picoband&reg;Identification of CCL26 as a differentially expressed prognostic gene. A – B CCL26 was significantly upregulated in HCC samples compared to both paired ( A ) and non-paired ( B ) normal liver samples from TCGA-LIHC cohort. C CCL26 expression is significantly higher in HCC tissues than in matched non-tumor tissues from the external cohort. D Relationship between CCL26 expression and clinical characteristics of patients with HCC. E ROC analysis illustrated that CCL26 expression accurately distinguished HCC tumor samples from normal samples (AUC = 0.717, 95% CI 0.671–0.763). F Kaplan–Meier curves of the CCL26 high and CCL26 low groups in the TCGA-LIHC cohort. ***p < 0.001. G Univariate and multivariate Cox regression analyses confirmed that CCL26 was an independent predictor of OS in the TCGA-LIHC cohort; HCC Hepatocellular carcinoma, OS overall survival, ROC receiver operating characteristic <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s12672-025-02280-1'>40205283</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9066-12672_2025_2280_fig3_html.pngAnti-Eotaxin 3/CCL26 Antibody Picoband&reg;Construction and evaluation of radiomic models for predicting CCL26 expression in HCC. A – B The importance of the selected features in ( A ) the whole-tumor model and B the whole + peripheral-tumor model; C – D the association between the rad-score and CCL26 expression in ( C ) the whole radiomic model; D the whole + peripheral radiomic model; E – H ROC curves of the whole radiomic model and whole + peripheral radiomic model in E , F the training cohort and G , H the cross-validation cohort; I calibration curves and J precision‒recall curves of the whole radiomic model; K DCA for the radiomic model; and L ROC curves for predicting the OS of patients with HCC based on the rad-score. *p < 0.05, ***p < 0.001; ROC receiver operating characteristic, PR precision-recall, DCA decision curve analysis, AUC area under the curve <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s12672-025-02280-1'>40205283</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9066-3-WB-anti-eotaxin-3-picoband-antibody.jpgAnti-Eotaxin 3/CCL26 Antibody Picoband&reg; Western blot analysis of Eotaxin 3 using anti-Eotaxin 3 antibody (PB9066). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Colo320 whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human HT1080 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eotaxin 3 at approximately 11 kDa. The expected band size for Eotaxin 3 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cntf-picoband-trade-antibody-pb9067-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9067-1-IHC-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; IHC analysis of CNTF using anti-CNTF antibody (PB9067). <br> CNTF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CNTF Antibody (PB9067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9067-2-IHC-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; IHC analysis of CNTF using anti-CNTF antibody (PB9067). <br> CNTF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CNTF Antibody (PB9067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9067-3-WB-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; Western blot analysis of CNTF using anti-CNTF antibody (PB9067). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat PC-12 whole cell lysates, <br> Lane 2: rat NRK whole cell lysates, <br> Lane 3: rat RH-35 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: rat spleen tissue lysates, <br> Lane 7: rat testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody (Catalog # PB9067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNTF at approximately 23 kDa. The expected band size for CNTF is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gdnf-picoband-trade-antibody-pb9069-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9069-1-WB-anti-gdnf-picoband-antibody.jpgAnti-GDNF Antibody Picoband&reg; Western blot analysis of GDNF using anti-GDNF antibody (PB9069). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDNF antigen affinity purified polyclonal antibody (Catalog # PB9069) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDNF at approximately 24 kDa. The expected band size for GDNF is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p27-kip-1-picoband-trade-antibody-pb9070-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9070-cdkn1b-primary-antibodies-fcm-testing-3.jpgAnti-p27 KIP 1/CDKN1B Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1/CDKN1B antibody (PB9070). <br>Overlay histogram showing SiHa cells stained with PB9070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9070-cdkn1b-primary-antibodies-wb-testing-1.jpgAnti-p27 KIP 1/CDKN1B Antibody Picoband&reg; Western blot analysis of p27 KIP 1/CDKN1B using anti-p27 KIP 1/CDKN1B antibody (PB9070). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p27 KIP 1/CDKN1B antigen affinity purified polyclonal antibody (Catalog # PB9070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p27 KIP 1/CDKN1B at approximately 27 kDa. The expected band size for p27 KIP 1/CDKN1B is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9070-cdkn1b-primary-antibodies-if-testing-2.jpgAnti-p27 KIP 1/CDKN1B Antibody Picoband&reg; IF analysis of p27 KIP 1/CDKN1B using anti-p27 KIP 1/CDKN1B antibody (PB9070). <br> p27 KIP 1/CDKN1B was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegf-picoband-trade-antibody-pb9071-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9071-oncotarget-07-8043-g002.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Lentivirus-mediated PLCγ1 shRNA could suppress migration in human gastric adenocarcinoma BGC-823 cells. Cells were transduced with lentivirus-mediated PLCγ1 shRNA2/3 vectors. ( A ) The formation of membrane ruffles was detected using Ruffling assay as described in Materials and Methods. The cell nuclei were stained DAPI (blue) and the membrane ruffles were stained rhodamine-conjugated phalloidin (red). Scale bar = 10 μm. ( B and C ) The migration ability was measured using Transwell assay (B, magnification × 100) and Scratch assay (C, magnification × 400) as described in Materials and Methods. ( D ) The protein levels of MMP2, MMP9, E-cadherin, N-cadherin, snail, slug, and GAPDH were detected with Western blotting analysis, and the pro and active forms of MMP2/9 were observed using gelatin zymography assay as described in Materials and Methods. ( E ) The mRNA levels of PLCG1, MMP2, MMP9, CDHI, CDH2, SNAIL, SLUG, and GAPDH were detected using Real-time PCR analysis as described in Materials and Methods. ( F ) The level of VEGF in extracellular matrix was detected using ELISA as described in Materials and Methods. Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4884974/&orig_host=www.ncbi.nlm.nih.gov'>26811493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9071-oncotarget-07-8043-g003.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Depletion of PLCγ1 suppresses growth and metastasis of gastric adenocarcinoma in a nude mouse tumor xenograft model. ( A ) Volume and weight of tumor samples from nude mice. ( B ) The protein levels of PCNA, cleaved-PARP, PARP, and Bcl-2 in the tumor samples were detected by Immunohistochemistry (Magnificationx100, x400) and Western blotting analyses as described in Materials and Methods. ( C ) The levels of MMP2 and MMP9 in the tumor samples were detected by Immunohistochemistry analysis as described in Materials and Methods (Magnificationx100, x400). The protein and mRNA levels of MMP2, MMP9, E-cadherin(CDH1), N-cadherin(CDH2), snail(SNAIL), and slug(SLUG) in the tumor samples were detected by Western Blotting and Real-time PCR analyses as described in Materials and Methods. ( D ) The protein levels of VEGF and CD34 and the mRNA level of VEGF in tumor samples were detected by Immunohistochemistry and Real-time PCR analysis as described in Materials and Methods. The number of microvessels was accounted under OLYPUS x41 microscope (Magnification x100, x400). ( E ) The lymphoid follicles in inguinal lymph nodes of nude mice were observed under OLYPUS x41microscope, and the protein levels of CD44 and GFP in inguinal lymph nodes of nude mice was detected by Immunohistochemistry analysis as described in Materials and Methods (Magnificationx40, x400). Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4884974/&orig_host=www.ncbi.nlm.nih.gov'>26811493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9071-vegfa-primary-antibodies-wb-testing-1.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: recombinant human VEGF/VEGFA protein 20ng, <br> Lane 2: recombinant human VEGF/VEGFA protein 10ng, <br> Lane 3: human U87 whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat C6 whole cell lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse Neuro-2a whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (PB9071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VEGF/VEGFA at approximately 18, 27 kDa. The expected band size for VEGF/VEGFA is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9071-vegfa-primary-antibodies-ihc-testing-1.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). <br> VEGF/VEGFA was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGF/VEGFA Antibody (PB9071) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9071-vegfa-primary-antibodies-ihc-testing-2.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). <br> VEGF/VEGFA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGF/VEGFA Antibody (PB9071) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9071-vegfa-primary-antibodies-fcm-testing-1.jpgAnti-VEGF/VEGFA Antibody Picoband&reg;Flow Cytometry analysis of K562 cells using anti-VEGF/VEGFA antibody (PB9071). <br> Overlay histogram showing K562 cells stained with PB9071 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alox5-antibody-rp1031-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9072-1-RP1031-IHC-anti-alox5-5-lo-antibody.jpgAnti-5 Lipoxygenase/ALOX5 Antibody Picoband&reg;Anti-ALOX5 Picoband antibody&#44; RP1031-1.JPG<br>IHC(P): Mouse Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9072-2-RP1031-IHC-anti-alox5-5-lo-antibody.jpgAnti-5 Lipoxygenase/ALOX5 Antibody Picoband&reg;Anti-ALOX5 Picoband antibody&#44; RP1031-2.JPG<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9072-3-RP1031-IHC-anti-alox5-5-lo-antibody.jpgAnti-5 Lipoxygenase/ALOX5 Antibody Picoband&reg;Anti-ALOX5 Picoband antibody&#44; RP1031-3.JPG<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9072-4-RP1031-IHC-anti-alox5-5-lo-antibody.jpgAnti-5 Lipoxygenase/ALOX5 Antibody Picoband&reg;Anti-ALOX5 Picoband antibody&#44; RP1031-4.JPG<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9072-5-RP1031-WB-anti-alox5-5-lo-antibody.jpgAnti-5 Lipoxygenase/ALOX5 Antibody Picoband&reg;Anti-ALOX5 Picoband antibody&#44; RP1031-5.jpg<br>All lanes: Anti-ALOX5 (RP1031) at 0.5ug/ml<br>WB: SGC Whole Cell Lysate at 40ug<br>Predicted bind size: 78KD<br>Observed bind size: 78KD<br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alox15-picoband-trade-antibody-pb9073-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9073-1-IHC-anti-alox15-15-lipoxygenase-1-picoband-antibody.jpgAnti-15 Lipoxygenase 1/ALOX15 Antibody Picoband&reg; IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073). <br> ALOX15 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9073-2-IHC-anti-alox15-15-lipoxygenase-1-picoband-antibody.jpgAnti-15 Lipoxygenase 1/ALOX15 Antibody Picoband&reg; IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073). <br> ALOX15 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9073-3-IHC-anti-alox15-15-lipoxygenase-1-picoband-antibody.jpgAnti-15 Lipoxygenase 1/ALOX15 Antibody Picoband&reg; IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073). <br> ALOX15 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9073-4-IHC-anti-alox15-15-lipoxygenase-1-picoband-antibody.jpgAnti-15 Lipoxygenase 1/ALOX15 Antibody Picoband&reg; IHC analysis of ALOX15 using anti-ALOX15 antibody (PB9073). <br> ALOX15 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALOX15 Antibody (PB9073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9073-alox15-primary-antibodies-wb-testing-5.jpgAnti-15 Lipoxygenase 1/ALOX15 Antibody Picoband&reg; Western blot analysis of ALOX15 using anti-ALOX15 antibody (PB9073). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: rat thymus tissue lysates, <br> Lane 3: rat spleen tissue lysates, <br> Lane 4: mouse lung tissue lysates, <br> Lane 5: mouse testis tissue lysates, <br> Lane 6: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX15 antigen affinity purified polyclonal antibody (Catalog # PB9073) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALOX15 at approximately 75 kDa. The expected band size for ALOX15 is at 75 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-bdnf-picoband-trade-antibody-pb9075-boster.html2026-03-26T05:19:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-41598_2018_27787_fig4_html.jpgAnti-BDNF Antibody Picoband&reg;( A ) Correlation between AGE and RAGE in different layers; ( B ) Correlation between TGF-β1 and TGF-β1receptor in different layers; ( C ) Correlation between BDNF and TrkB in different layers; ( D ) Correlation between RAGE and TGF-β1receptor in different layers. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-41598_2018_27787_fig3_html.jpgAnti-BDNF Antibody Picoband&reg;( A ) Correlation between AGE and RAGE in muscle layer and submucosa layer with circumferential constant a; ( B ) Correlation between AGE and RAGE in muscle layer and submucosa layer with longitudinal constant a; ( C ) Correlation between TGF-β1 and TGF-β1 receptor in mucosa layer and TGF-β1 muscle layer with circumferential and longitudinal material constant a; ( D ) Correlation between BDNF in muscle and submucosa layers with longitudinal constant a. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-41598_2018_27787_fig2_html.jpgAnti-BDNF Antibody Picoband&reg;The fraction of AGE, RAGE, TGF-β1, TGF- β1 receptor, BDNF and TrkB in the different layers of the colon between two groups. In the different layers, the fraction of AGE, RAGE, TGF-β1 and TGF- β1 receptor was bigger whereas the fraction of BDNF and TrkB was smaller in the Diabetes group than in Control group. Compared with Control group: *P < 0.05, **P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-41598_2018_27787_fig1_html.jpgAnti-BDNF Antibody Picoband&reg;The representative samples of immunohistochemical staining for AGE, RAGE, TGF-β1, TGF- β1 receptor, BDNF and TrkB in the colon wall of two groups. The microscopy with high magnification have been inserted in each single histological photo (arrow) in order to display the localization of markers. The staining of all proteins was stronger in the muscle layer than other layers. In the different layers, the staining of AGE, RAGE, TGF-β1 and TGF- β1 receptor was stronger whereas the staining of BDNF and TrkB was weaker in the Diabetes group than in Control group. Bar = 100 um. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-27787-2'>29930382</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-12951_2022_1337_fig9_html.pngAnti-BDNF Antibody Picoband&reg;SPION-mediated magnetic actuation upregulates the expression of neurotrophic factors associated with repair phenotypes in Schwann cells. The protein expression of repair phenotype-related neurotrophic factors BDNF, GDNF, Olig1 and VEGF in different experimental groups was detected by immunohistochemical staining at 3 (a) , 7 (d) , 14 (g) and 21 days (j) after crush injury, and the protein expression levels were quantitatively analyzed ( b , e , h , k ). c , f , i , l The protein expression levels of such neurotrophic factors at the above time points were detected by ELISA, and the results were consistent with the immunohistochemical analysis. Each experiment was carried out in triplicate. The values are represented as the mean ± SD. Scale bar = 50 µm in panels a , d , g , j . * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-022-01337-5'>35351151</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-bdnf-primary-antibodies-ihc-testing-4.jpgAnti-BDNF Antibody Picoband&reg;IHC analysis of BDNF using anti-BDNF antibody (PB9075). <br>BDNF was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-bdnf-primary-antibodies-wb-testing-1.jpgAnti-BDNF Antibody Picoband&reg; Western blot analysis of BDNF using anti-BDNF antibody (PB9075). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human BDNF protein 5 ng,<br> Lane 2: recombinant human BDNF protein 2.5 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDNF antigen affinity purified polyclonal antibody (PB9075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BDNF at approximately 14 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-bdnf-primary-antibodies-wb-review-1.pngAnti-BDNF Antibody Picoband&reg; Western blot analysis of BDNF using anti-BDNF antibody (PB9075). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: normal group-Hippocampal tissue lysates from normal mouse tissue lysates,<br> Lane 2: model group-Hippocampal tissue lysates from depressed mouse,<br> Lane 3: low-dose group-Hippocampal tissue lysates from depressed mouse treated with an in-house drug,<br> Lane 4: high-dose group-Hippocampal tissue lysates from depressed mouse treated with an in-house drug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDNF antigen affinity purified polyclonal antibody (PB9075) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BDNF at approximately 28 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-bdnf-primary-antibodies-ihc-testing-2.jpgAnti-BDNF Antibody Picoband&reg; IHC analysis of BDNF using anti-BDNF antibody (PB9075). <br> BDNF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9075-bdnf-primary-antibodies-ihc-testing-3.jpgAnti-BDNF Antibody Picoband&reg; IHC analysis of BDNF using anti-BDNF antibody (PB9075). <br> BDNF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-beclin-1-picoband-trade-antibody-pb9076-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-beclin-1-primary-antibodies-wb-testing-1.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg; Western blot analysis of Beclin 1 using anti-Beclin 1 antibody (PB9076). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beclin 1 antigen affinity purified polyclonal antibody (Catalog # PB9076) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beclin 1 at approximately 60 kDa. The expected band size for Beclin 1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-beclin-1-primary-antibodies-if-testing-2.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg; IF analysis of Beclin 1 using anti-Beclin 1 antibody (PB9076). <br> Beclin 1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Beclin 1 Antibody (PB9076) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-fphar-16-1526653-g007.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg;AEA improved CHF via PI3K/AKT/Bnip3 axis. (A) Representative images of PI3K/AKT axis. (B, C) The phosphorylation level of PI3K and AKT. (D) Representative images of Opa1, Drp1, Bnip3, p62, Atg5 and LC3II. (E–J) The expression level of Opa1, Drp1, Bnip3, p62, Atg5 and LC3II. (n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1526653/full'>40206063</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-fphar-16-1526653-g010.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg;AEA improved NE-induced injuries via PI3K/AKT/Bnip3 axis. (A) Representative images of PI3K/AKT axis in H9c2 cells. (B, C) The phosphorylation level of PI3K and AKT. (D) Representative images of Opa1, Drp1, TrxR2, Bnip3, p62, Atg5 and LC3II in cells. (E–K) The expression level of Opa1, Drp1, TrxR2, Bnip3, p62, Atg5 and LC3II in H9c2 cells. (n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1526653/full'>40206063</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-fphar-09-00301-g004.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg;Effects of LLC on autophagy in the hearts from rats subjected to myocardial ischemia injury. (A) Representative transmission electron ultra-images showing autophagic vacuoles (marked with red arrows in the images, scale bar = 0.5 μm). (B) Quantitative analysis of the number of autophagic vacuoles in (A) . (C) Expression levels of LC3, Beclin 1 and p62 changes with LLC (80 mg⋅Kg -1 ) pretreatment. Data were presented as means ± SD. ∗∗ P < 0.01 vs. Con group, # P < 0.05, ## P < 0.01 vs. ISO group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00301/full'>29651246</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-fphar-09-00301-g005.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg;Effects of LLC on autophagy in H9c2 cardiomyocytes under OGD. (A) Expression levels of LC3, Beclin 1 and p62 changes with LLC pretreatment. (B) Expression levels of LC3 and p62 changes in the presence or absence of 5 μmol⋅L -1 chloroquine and 40 μg⋅mL -1 LLC. (C) H9c2 cardiomyocytes were transfected with mRFP-GFP-LC3 and observed by fluorescent microscope (scale bar = 10 μm). (D) Mean number of autophagosomes represented by yellow dots in merged images and autolysosomes represented by red dots in merged images per cell. All experiments were repeated at least three times. Data were presented as means ± SD. ∗∗ P < 0.01 vs. Con group, ## P < 0.01 vs. OGD group, & P < 0.05, && P < 0.01 vs. OGD+LLC group, $ P < 0.05 vs. OGD+CQ group. CQ, chloroquine.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00301/full'>29651246</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-fphar-09-00301-g006.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg;PI3K/Akt/mTOR pathway is involved in the cardioprotective effects of LLC. (A) Expression levels of PI3K, p-Akt, Akt, p-mTOR and mTOR in hearts were analyzed. (B) Representative western blots of PI3K (p110α), p-Akt (Ser473), Akt, p-mTOR (Ser2448), mTOR, LC3, Beclin 1 and p62 in the presence or absence of 20 μmol⋅L -1 LY294002 and 40 μg⋅mL -1 LLC. (C) The cell viability was analyzed by MTT assay. (D) The cell injury was detected by LDH measurements. All experiments were repeated at least three times. Data were presented as means ± SD. ∗∗ P < 0.01 vs. Con group, ## P < 0.01 vs. OGD group, & P < 0.05, && P < 0.01 vs. OGD+LLC group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00301/full'>29651246</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-12974_2025_3493_fig9_html.pngAnti-Beclin 1/BECN1 Antibody Picoband&reg;Effects of increasing endogenous irisin by FAE on autophagy and AMPK/mTOR pathway post CCH. A-D Western blot analysis for autophagy protein levels, including Beclin 1, SQSTM1, MAP1LC3B-I and MAP1LC3B-II in brain, with quantification (band intensity normalized to β-actin) ( n = 6/group). A , E-H Western blot analysis for phosphorylation levels of AMPK and mTOR in brain, with quantification (band intensity normalized to β-actin) ( n = 6/group). One-way ANOVA, Tukey post hoc test ( B–H ). The data represent the mean ± SD, p < 0.05 was set as the threshold for significance. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, no significance, as indicated Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-025-03493-5'>40581647</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-beclin-1-primary-antibodies-fcm-testing-3.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg; Flow Cytometry analysis of Raji cells using anti-Beclin 1 antibody (PB9076). <br> Overlay histogram showing Raji cells stained with PB9076 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beclin 1 Antibody (PB9076, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-beclin-1-primary-antibodies-wb-testing-4.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg; Western blot analysis of Beclin 1 using anti-Beclin 1 antibody (PB9076). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: rat C6 whole cell lysates, <br> Lane 5: rat PC-12 whole cell lysates, <br> Lane 6: mouse NIH/3T3 whole cell lysates,<br> Lane 7: mouse Raw264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beclin 1 antigen affinity purified polyclonal antibody (PB9076) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for Beclin 1 at approximately 60 kDa. The expected band size for Beclin 1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9076-becn1-primary-antibodies-ip-testing-5_1.jpgAnti-Beclin 1/BECN1 Antibody Picoband&reg; Immunoprecipitating Beclin 1/BECN1 in MCF-7 whole cell lysate.<br> Western blot analysis of Beclin 1/BECN1 using anti-Beclin 1/BECN1 antibody (PB9076); <br> Lane 1: MCF-7 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Beclin 1/BECN1 antibody in MCF-7 whole cell lysate;<br> Lane 3: anti-Beclin 1/BECN1 antibody (2μg) + MCF-7 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beclin 1/BECN1 antigen affinity purified polyclonal antibody (PB9076) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Beclin 1/BECN1 at approximately 60 kDa. The expected band size for Beclin 1/BECN1 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cbl-picoband-trade-antibody-pb9077-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9077-cbl-primary-antibodies-wb-testing-1.jpgAnti-CBL Antibody Picoband&reg; Western blot analysis of CBL using anti-CBL antibody (PB9077). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: rat RH35 whole cell lysates, <br> Lane 6: mouse Ana-1 whole cell lysates, <br> Lane 7: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBL antigen affinity purified polyclonal antibody (Catalog # PB9077) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBL at approximately 120 kDa. The expected band size for CBL is at 100,120 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9077-2_1.jpgAnti-CBL Antibody Picoband&reg; IHC analysis of CBL using anti-CBL antibody (PB9077). <br> CBL was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CBL Antibody (PB9077) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9077-3.jpgAnti-CBL Antibody Picoband&reg; IHC analysis of CBL using anti-CBL antibody (PB9077). <br> CBL was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CBL Antibody (PB9077) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9077-4.jpgAnti-CBL Antibody Picoband&reg; IHC analysis of CBL using anti-CBL antibody (PB9077). <br> CBL was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CBL Antibody (PB9077) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9077-5.jpgAnti-CBL Antibody Picoband&reg; IF analysis of CBL using anti-CBL antibody (PB9077). <br> CBL was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CBL Antibody (PB9077) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9077-6.jpgAnti-CBL Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-CBL antibody (PB9077). <br>Overlay histogram showing A549 cells stained with PB9077 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CBL Antibody (PB9077, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcr3-picoband-trade-antibody-pb9079-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9079-1-IHC-anti-cxcr3-picoband-antibody.jpgAnti-CXCR3 Antibody Picoband&reg; IHC analysis of CXCR3 using anti-CXCR3 antibody (PB9079). <br> CXCR3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CXCR3 Antibody (PB9079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9079-2-IHC-anti-cxcr3-picoband-antibody.jpgAnti-CXCR3 Antibody Picoband&reg; IHC analysis of CXCR3 using anti-CXCR3 antibody (PB9079). <br> CXCR3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CXCR3 Antibody (PB9079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9079-fonc-11-629350-g003.jpgAnti-CXCR3 Antibody Picoband&reg;Through transwell co-culture with HPV+ cells (SiHa and Caski), PD-L1 expression in HFF-1 cells were induced by CXCL10-CXCR3 interaction, while were decreased after co-culture with the HPV+ cells which E6 and E7 has been knocked down or treatment with AMG487. (A) Immunohistochemistry revealed that the expression of PD-L1 had a strong relationship with HPV infection in cervix tissues, which showed the higher expression of PD-L1 in CSCC (HPV+ Group) than that of a normal cervical epithelium (HPV- Group); (B) The expression of PD-L1, JAK1 and pSTAT1 in different cells was tested by western blotting. The band intensities were calculated using the ImageJ software. GAPDH was used as an internal control for the total protein measurement. The ratio of the target gene to GAPDH was used to conduct the statistical analysis. (C, D) . Compared to the co-culture with HFF-1 cells, the expression levels of PD-L1, JAK1 and pSTAT1 in HFF-1 cells were all upregulated following co-culture with HPV+ cells (SiHa, Caski) while the upregulation of JAK1 and pSTAT1 were diminished after co-culture with koE6/E7Siha and SiE6Caski cells or treatment with AMG487 using the 0.4 µm polycarbonate membrane transwell assay, in which cells could not pass through. *P < 0.05, **P < 0.01, ***P<0.001 and ****P < 0.0001, as determined by Student’s t-test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.629350/full'>34422627</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9079-fonc-11-629350-g004.jpgAnti-CXCR3 Antibody Picoband&reg;CXCL10-CXCR3 upregulated PD-L1 expression by activating the JAK1-STAT pathway in HPV+ cervical cancer cells and tissues. (A) Map of chemokine signaling pathways generated by KEGG pathway analysis in DAVID by RNA-seq from Aksomics company; (B) The results of pan-phosphorylation western blot detection on the cells including HFF-1, HFF-1 co-cultured with SiHa, KoE6/E7 SiHa, and KoE6E7SiHa co-cultured with SiHa cells. (C–F) The expression of PD-L1, JAK1 and STAT1 in HFF-1 or KoE6/E7 SiHa cells after treatment with recombinant human CXCL10, AMG487 or ruxonitilib, to further verify the HPV E6/E7 can induce upregulated expression of PD-L1 in CSCC through CXCL10 binding to CXCR3 which leads to JAK-STAT pathway activation. *P < 0.05, **P < 0.01, ***P<0.001 and ****P < 0.0001, as determined by Student’s t-test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.629350/full'>34422627</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9079-3-IHC-anti-cxcr3-picoband-antibody.jpgAnti-CXCR3 Antibody Picoband&reg; IHC analysis of CXCR3 using anti-CXCR3 antibody (PB9079). <br> CXCR3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CXCR3 Antibody (PB9079) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9079-4-WB-anti-cxcr3-picoband-antibody.jpgAnti-CXCR3 Antibody Picoband&reg; Western blot analysis of CXCR3 using anti-CXCR3 antibody (PB9079). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Colo320 whole cell lysates, <br> Lane 2: human SGC whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR3 antigen affinity purified polyclonal antibody (Catalog # PB9079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCR3 at approximately 41 kDa. The expected band size for CXCR3 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-foxp3-antibody-rp1032-boster.html2026-03-24T05:04:33+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1032-fimmu-14-1238694-g001.jpgAnti-Forkhead box protein P3 FOXP3 AntibodyImmunohistochemistry findings from tissue samples of CRC patients before and after MTE treatment. (A) Shown are the findings from one patient. Results of other 16 patients are shown in Figure S1 . (B) The bar graph compares the expression of PD-1, PD-L1, TGF-β1(tumor), and FOXP3 before and after MTE treatment in all 17 patients. (C) Serum levels of IL-2, IL-10 and TGF-β1 were compared before and after treatment in all 17 patients. Cytokine concentrations were normalized against the untreated group. * P < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10465246/&orig_host=www.ncbi.nlm.nih.gov'>37649480</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1032-fimmu-14-1238694-g003.jpgAnti-Forkhead box protein P3 FOXP3 AntibodyWestern blotting and ELISA of HCT116, LoVo and Jurkat T cells treated with MTE treated in single culture or co-culture condition. (A) Western blot analysis of PD-L1, TGF-β1 expression in HCT116 or LoVo cells, single cultured or co-cultured with Jurkat T cells and treated with MTE, which were labeled in top row of blots or bottom row of column chart. (B) Western blot analysis of PD-1 and FOXP3 expression in Jurkat T cells single cultured or co-cultured with HCT116 or LoVo cells, and treated with MTE, which were labeled in top row of blots or bottom row of column chart. (C) ELISA analysis of supernatants from single cultures or co-cultures. In single culture, supernatants of HCT116 or LoVo cells was used to test TGF-β1 secretion, and supernatants of Jurkat T cells was used to test IL-2 and IL-10 secretion levels.* P < 0.05, ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10465246/&orig_host=www.ncbi.nlm.nih.gov'>37649480</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1032-foxp3-primary-antibodies-ihc-testing-1.jpgAnti-Forkhead box protein P3 FOXP3 Antibody IHC analysis of FOXP3 using anti-FOXP3 antibody (RP1032). <br> FOXP3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXP3 Antibody (RP1032) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1032-foxp3-primary-antibodies-ihc-testing-2.jpgAnti-Forkhead box protein P3 FOXP3 Antibody IHC analysis of FOXP3 using anti-FOXP3 antibody (RP1032). <br> FOXP3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXP3 Antibody (RP1032) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-galectin-3-picoband-trade-antibody-pb9081-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9081-1-IHC-anti-galectin-3-lgals3-antibody.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; IHC analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081).<br> Galectin-3 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Galectin-3 Antibody (PB9081) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9081-2-IHC-anti-galectin-3-lgals3-antibody.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; IHC analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081).<br> Galectin-3 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Galectin-3 Antibody (PB9081) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9081-galectin-3-primary-antibodies-wb-testing-3.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; Western blot analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human SiHa whole cell lysates, <br> Lane 3: human U87 whole cell lysates, <br> Lane 4: human Hacat whole cell lysates, <br> Lane 5: human PC-3 whole cell lysates, <br> Lane 6: human T47D whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3 antigen affinity purified polyclonal antibody (Catalog # PB9081) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin-3 at approximately 26 kDa. The expected band size for Galectin-3 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9081-4.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; IF analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081).<br> Galectin-3 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Galectin-3 Antibody (PB9081) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9081-5.pngAnti-Galectin 3/LGALS3 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Galectin-3 antibody (PB9081). <br> Overlay histogram showing U20S cells stained with PB9081 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Galectin-3 Antibody (PB9081&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9081-galectin-3-primary-antibodies-if-testing-6.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; IF analysis of Galectin-3 using anti-Galectin-3 antibody (PB9081). <br> Galectin-3 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Galectin-3 Antibody (PB9081) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gfap-picoband-trade-antibody-pb9082-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-13046_2010_article_360_fig6_html.jpgAnti-GFAP Antibody Picoband&reg;Immunofluorescence staining of differentiated BTSCs for GFAP (FITC, × 200) . 6A: DAPI. 6B:GFAP. 6C:Merge. It showed the differentiated BTSCs induced by ATRA were GFAP positive. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-29-113'>20716331</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-ihc-testing-7.jpgAnti-GFAP Antibody Picoband&reg;IHC analysis of GFAP using anti-GFAP antibody (PB9082). <br>GFAP was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-ymj-62-215-g001.jpgAnti-GFAP Antibody Picoband&reg;Evaluation of lncRNA Zfas1 expression in tissues and cell lines. (A) Expression of lncRNA Zfas1 was detected in serum of epilepsy patients and healthy controls. * p <0.05 when compared with NC group. (B) Serum levels of lncRNA Zfas1 were correlative with serum levels of Bax, Bcl-2, and Caspase-3. (C) Serum levels of lncRNA Zfas1 showed an association with IL-2, TNF-α, IFN-γ and HMGB-1 levels. (D) Serum levels of lncRNA Zfas1 were altered with changes in S100B, NSE, GFAP, and CGRP levels. IL, interleukin; TNF, tumor necrosis factor; IFN, Interferon; HMGB, high mobility group box protein; NSE, neuron specific enolase; GFAP, glial fibrillary acidic protein; CGRP, calcitonin gene related peptide; NC, negative control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7934098/&orig_host=www.ncbi.nlm.nih.gov'>33635011</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-nrr-16-1660-g012.jpgAnti-GFAP Antibody Picoband&reg; Effects of exendin-4 and DA-CH5 on 6-OHDA-induced alterations in astrocyte activation, striatal TNF-α levels, and pro-inflammatory cytokine levels in the rat striatum. (A) Representative fluorescence images of GFAP (green, stained by fluorescein isothiocyanate) in the striatum. Scale bar: 100 µm. (B) Quantification of GFAP-positive cells in the striatum. (C) Levels of TNF-α in striatum homogenate as quantified by enzyme-linked immunosorbent assay. (D) Bands of IL-1β and TNF-α in the striatum. (E, F) Quantification of IL-1β (E) and TNF-α (F) expression in the striatum. Data are expressed as the mean ± SD of four rats per group (A–B and D–F) or six rats per group (C). ‡ P < 0.05, ‡‡ P < 0.01, ‡‡‡ P < 0.001, vs . sham group; $ P < 0.05, $$ P < 0.01, $$$ P < 0.001, vs . 6-OHDA group; &P < 0.05, vs . 6-OHDA + exendin-4 group (one-way analysis of variance followed by Tukey’s multiple comparison test). 6-OHDA: 6-Hydroxydopamine; exendin-4 and DA-CH5: glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; IL-1β: interleukin-1β; PD: Parkinson’s disease; SN: substantia nigra; TNF-α: tumor necrosis factor-α.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8323666/&orig_host=www.ncbi.nlm.nih.gov'>33433498</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-ajtr0011-2925-f3.jpgAnti-GFAP Antibody Picoband&reg;Localization of central AGT and AT1 receptors and Blood brain barrier permeability. A. Localization of central AGT and AT1 receptors determined by doublestaining with the antibodies against AGT or AT1 receptors (green) and the antibodies-recognized NSE or GFAP (red). NSE, neuron-specific enolase; GFAP, glial fibrillary acidic protein. B. Blood brain barrier permeability was up-regulated in DM rats (b1), but there was no significant difference in all intervention groups (b2).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-nrr-14-289-g003.jpgAnti-GFAP Antibody Picoband&reg;Morphological and immunocytochemical characteristics of the hippocampal NVU. (A) Hippocampal neurons, astrocytes and microvascular endothelial cells under an inverted light microscope. (B) Neurons are positive for NSE (arrows), astrocytes express GFAP (arrows), and brain microvascular endothelial cells are positive for CD31 (arrows). Scale bars: 100 μm. NVU: Neurovascular unit; NSE: neuron-specific enolase; GFAP: glial fibrillary acidic protein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6301159/&orig_host=www.ncbi.nlm.nih.gov'>30531012</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-nrr-14-289-g007.jpgAnti-GFAP Antibody Picoband&reg;Expression of CX43 and GFAP proteins in the hippocampal NVU triple cell co-culture system and in the AS + BM co-culture system, with or without glucose (150 mM) and corticosterone (200 μM), as detected by western blot assay. Results are shown as the mean ± SD ( n = 3; one-way analysis of variance followed by Dunnett’s post hoc test). * P < 0.05, ** P < 0.01, vs . NVU group; # P < 0.05, vs . NVU model group (NVU + glucose + corticosterone). Western blot assay was performed in triplicate. GAPDH was used as loading control. Numbers on the right indicate the molecular weights of the proteins (kDa). NE: Neuron; AS: astrocyte; BM: brain microvascular endothelial cell; NVU: neurovascular unit; CX43: connexin 43; GFAP: glial fibrillary acidic protein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6301159/&orig_host=www.ncbi.nlm.nih.gov'>30531012</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-ijcep0011-3119-f1.jpgAnti-GFAP Antibody Picoband&reg;Fluorescent immunocytochemistry of cultured Schwann cells stained by S-100 (A) and GFAP (B), with nuclei counterstained with DAPI. (C) Effects of different CXCL12 concentration on Schwann cell proliferation performed by CCK-8 assay. Data are presented as mean ± SD, n = 3. One-way ANOVA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6958085/&orig_host=www.ncbi.nlm.nih.gov'>31938440</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-djir_a_12469679_f0005_c.jpgAnti-GFAP Antibody Picoband&reg;1 Hz rTSMS reduces astrocyte activation and neuroinflammation. (A) Representative immunofluorescence images showing GFAP (red) and Dapi (blue) staining in the injured spinal cord across groups. (B) Quantification of mean GFAP fluorescence intensity. (C) Western blot analysis of GFAP and BDNF expression. (D and E) Quantification of GFAP and BDNF relative to β-actin. (F) Western blot analysis of pro-inflammatory cytokines (TNF-α, IFN-γ and IL-6) and anti-inflammatory cytokines (IL-4 and IL-10). (G–K) Corresponding quantification of cytokine expression. n=5. Scale bar=20 μm. aP < 0.05, bP< 0.01, cP < 0.001, vs Sham group. dP < 0.05, eP < 0.01, fP < 0.001, vs SCI group. gP < 0.05, hP <0.01, vs 1 Hz group. The data are expressed as the mean ± SD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/JIR.S516006'>40873779</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-fbioe-13-1655686-g003.jpgAnti-GFAP Antibody Picoband&reg;Evaluation of NF and GFAP in the spinal cord tissues and assessment of behavior function. (A) Representative fluorescent micrographs of immunofluorescence staining of NF (green) and GFAP (red) at Day 35 in spinal cord tissues of CBT-gel treatment and SCI group. Nuclei were stained by DAPI (Blue). (B–D) Tissues from the lesion epicenter (B) and adjacent (C) regions were quantified for positive areas of NF and GFAP, and the NF/GFAP ratios were calculated (D) . NF/GFAP ratio = (NF + area)/(GFAP + area in same region). (E) BBB scores of the animals during the 35-day recovery. Data are presented as mean and SD (n = 6–8). Statistical analysis was assessed by Mann-Whitney U test *p < 0.05, **p < 0.01, ***p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2025.1655686/full'>40964438</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-wb-testing-1_1.jpgAnti-GFAP Antibody Picoband&reg; Western blot analysis of GFAP using anti-GFAP antibody (PB9082). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: rat C5 whole cell lysates, <br> Lane 5: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (Catalog # PB9082) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-ihc-testing-2.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PB9082). <br> GFAP was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-ihc-testing-3.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PB9082). <br> GFAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-ihc-testing-4.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PB9082). <br> GFAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-ihc-testing-5.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PB9082). <br> GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-ihc-testing-6.jpgAnti-GFAP Antibody Picoband&reg; IHC analysis of GFAP using anti-GFAP antibody (PB9082). <br> GFAP was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9082-gfap-primary-antibodies-if-testing-7.jpgAnti-GFAP Antibody Picoband&reg; IF analysis of GFAP using anti-GFAP antibody (PB9082). <br> GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmox1-picoband-trade-antibody-pb9085-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9085-fphar-10-01459-g007.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg;Effects of magnolol on mice alcohol-induced liver damage in the Nrf2/HO-1 signaling pathway. After the completion of modeling and samples were collected, the liver of mice was lysed to detect the proteins by western blotting analysis. The levels of Nrf2 and HO-1 were compared with GAPDH. The data were demonstrated as means ± SD. (*P < 0.05, ***P < 0.001 and "ns" means not significant).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.01459/full'>31920652</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9085-fphar-10-00175-g007.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg;Trimetazidine restrained EE-induced activation of NF-κB and inactivation of HO-1/Nrf2 signaling pathways. (A–C) Western blot analysis of the protein levels of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα), P-IκBα, and nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB) in myocardial tissues. GAPDH and Histone H3 were used as loading controls. (D,E) The DNA binding activity of NF-κB was evaluated by EMSA assay. (F–G) Western blot analysis of the protein levels of heme oxygenase 1 (HO-1) and nuclear factor (erythroid-derived 2)-like 2 (Nrf2) in myocardial tissues. Each value is shown as mean ± SD ( n = 6). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, versus the indicated group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00175/full'>30890937</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9085-41598_2017_851_fig6_html.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg;Involvement of Nrf2/HO-1 signaling pathway in therapeutic effects of NMN. ( A ) Representative images and quantitative analysis of HO-1 protein in peri-hemorrhagic brain tissues at 24 hours after cICH. ( B ) Representative images and quantitative analysis of Nrf2 protein in peri-hemorrhagic brain tissues at 24 hours after cICH. ( C ) Representative images and quantitative analysis of Nrf2 protein distribution in cytosolic and nuclear extracts of brain peri-hemorrhagic tissues at 24 hours after cICH. * P < 0.01, n = 5 per group. NS, no significance. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-00851-z'>28386082</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9085-hmox1-primary-antibodies-wb-testing-1_1.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg;Western blot analysis of HMOX1 using anti-HMOX1 antibody (PB9085). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat PC-12 whole cell lysates,<br> Lane 4: mouse spleen tissue lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates,<br> Lane 6: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX1 antigen affinity purified polyclonal antibody (PB9085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMOX1 at approximately 33 kDa. The expected band size for HMOX1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9085-hmox1-primary-antibodies-ihc-testing-1.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg;IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9085). <br> HMOX1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9085-hmox1-primary-antibodies-ihc-testing-2_1.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg;IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9085). <br> HMOX1 was detected in a paraffin-embedded section of rat alcoholic hepatitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-itgb1-picoband-trade-antibody-pb9086-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9086-itgb1-primary-antibodies-wb-testing-1.jpgAnti-Integrin beta 1/ITGB1 Antibody Picoband&reg; Western blot analysis of ITGB1 using anti-ITGB1 antibody (PB9086). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: rat C6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB1 antigen affinity purified polyclonal antibody (Catalog # PB9086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGB1 at approximately 120-130 kDa. The expected band size for ITGB1 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9086-2_1-IHC-anti-itgb1-integrin-beta-1-picoband-antibody.jpgAnti-Integrin beta 1/ITGB1 Antibody Picoband&reg; IHC analysis of ITGB1 using anti-ITGB1 antibody (PB9086). <br> ITGB1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGB1 Antibody (PB9086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9086-itgb1-primary-antibodies-ihc-testing-3.jpgAnti-Integrin beta 1/ITGB1 Antibody Picoband&reg; IHC analysis of ITGB1 using anti-ITGB1 antibody (PB9086). <br> ITGB1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGB1 Antibody (PB9086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nf-kb-p65-picoband-trade-antibody-pb9087-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9087-40880_2018_334_fig2_html.pngAnti-NF-kB p65/RELA Antibody Picoband&reg;LMP1 up-regulated IL-2Rα expression through the MAPK/NF-κB pathway in NK-92 cells. a NK-92 cells transduced with LMP1-encoding lentivirus led to higher levels of p-Raf-B, p-p38, p-JNK, p-ERK, and p65 than transduction with NC lentivirus. b – f NK-92 cells transduced with LMP1-encoding lentivirus were treated for 1 h with the following selective inhibitors of proteins in the MAPK/NF-κB pathway, leading to IL-2Rα down-regulation: b 0.1 μM SB590885 (B-Raf inhibitor), c 20 μM PD98059 (ERK inhibitor), d 10 μM SB203580 (p38 inhibitor), e 20 μM SP600125 (JNK inhibitor), and f 100 μM pyrrolidine dithiocarbamate (PDTC; NF-κB inhibitor) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40880-018-0334-8'>30340635</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9087-40880_2018_334_fig3_html.pngAnti-NF-kB p65/RELA Antibody Picoband&reg;Treating SNK-6 cells constitutively expressing LMP1 for 1 h with the following selective inhibitors of proteins in the MAPK/NF-κB pathway down-regulated IL-2Rα: a 0.1 μM SB590885 (B-Raf inhibitor), b 20 μM PD98059 (ERK inhibitor), c 10 μM SB203580 (p38 inhibitor), d 20 μM SP600125 (JNK inhibitor), or e 100 μM pyrrolidine dithiocarbamate (PDTC, NF-κB inhibitor) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40880-018-0334-8'>30340635</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9087-medscimonit-24-1282-g003.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Knockdown of Src downregulated the expression of Fn14 and reduced the activation of NF-κB signaling in HCC827 cells. ( A ) Representative images and quantitative analysis of the effect of Src knockdown on the levels of Fn14 and the signaling molecules in the NF-κB pathway. Knockdown of Src induced the expression of Fn14, and elevated the levels of p-IκBα, p-IKKβ, and nuclear NF-κB p65. ( B ) Representative images of immunofluorescent detection of the effect of Src knockdown on the nuclear translocation of NF-κB p65. Silencing of Src restricted the translocation of NF-κB p65 into cell nuclei. * p <0.05 versus parental group. Scale bar, 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5846370/&orig_host=www.ncbi.nlm.nih.gov'>29500337</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9087-medscimonit-24-1282-g006.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Overexpression of Fn14 restored the activation of NF-κB signaling in Src -silenced HCC827 cells. ( A ) Representative images and quantitative analysis of the effect of Fn14 overexpression on the expression of Fn14 and the activation of the NF-κB signaling molecules in Src -silenced HCC827 cells. Overexpression of Fn14 elevated the levels of p-IκBα, p-IKKβ and nuclear NF-κB p65, representing the activation of Fn14-mediated NF-κB pathway in Src knockdown HCC827 cells. ( B ) Representative images of immunofluorescent detection of the effect of Fn14 overexpression on the nuclear translocation of NF-κB p65. Overexpression of Fn14 stimulated nuclear translocation of NF-κB p65. * p <0.05 versus the shSrc group. Scale bar, 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5846370/&orig_host=www.ncbi.nlm.nih.gov'>29500337</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9087-medscimonit-24-1282-g007.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Overexpression of Fn14 restored the activation of NF-κB signaling in Src -silenced A549 cells. ( A ) Representative images and quantitative analysis of the effect of Fn14 overexpression on the expression of Fn14 and the activation of the NF-κB signaling molecules in Src -silenced A549 cells. Overexpression of Fn14 elevated the levels of p-IκBα, p-IKKβ, and nuclear NF-κB p65, representing the activation of Fn14-mediated NF-κB pathway in Src knockdown A549 cells. ( B ) Representative images of immunofluorescent detection of the effect of Fn14 overexpression on the nuclear translocation of NF-κB p65. Overexpression of Fn14 stimulated nuclear translocation of NF-κB p65. * p <0.05 versus the shSrc group. Scale bar, 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5846370/&orig_host=www.ncbi.nlm.nih.gov'>29500337</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9087-medscimonit-24-1282-g008.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Knockdown of Src in NSCLC cells limited experimental metastases in vivo and inhibited Fn14-mediated NF-κB pathway. ( A ) H&E-stained lung sections and statistics of lung metastasis in BALB/c mice with tail vein injection of HCC827 cells with or without Src knockdown. ( B ) Representative images and quantitative analysis of the effect of Src knockdown on the expression of Fn14 and nuclear translocation of NF-κB p65 in vivo . *, p <0.05 versus the shSrc group. Scale bar, 200 μm. Arrows indicate metastatic tumors.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5846370/&orig_host=www.ncbi.nlm.nih.gov'>29500337</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9087-rela-primary-antibodies-wb-testing-1.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg; Western blot analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PB9087). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Caco-2 whole cell lysates,<br> Lane 4: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65/RELA antigen affinity purified polyclonal antibody (Catalog # PB9087) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65/RELA at approximately 65 kDa. The expected band size for NF-kB p65/RELA is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp90-alpha-picoband-trade-antibody-pb9089-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9089-1-IHC-anti-hsp90-alpha-picoband-antibody.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). <br> Hsp90 Alpha was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp90 Alpha Antibody (PB9089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-hsp90aa1-primary-antibodies-ihc-testing-1.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg;IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). <br>Hsp90 Alpha was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp90 Alpha Antibody (PB9089) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9089-4-IF-anti-hsp90-alpha-picoband-antibody.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; ICC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). <br> Hsp90 Alpha was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-Hsp90 Alpha Antibody (PB9089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-hsp90aa1-primary-antibodies-ihc-testing-2.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg;IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). <br>Hsp90 Alpha was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp90 Alpha Antibody (PB9089) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9089-5-IF-anti-hsp90-alpha-picoband-antibody.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; ICC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). <br> Hsp90 Alpha was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-Hsp90 Alpha Antibody (PB9089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-hsp90-primary-antibodies-wb-testing-6.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; Western blot analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody (PB9089). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat heart tissue lysates, <br> Lane 6: mouse brain tissue lysates, <br> Lane 7: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp90 Alpha antigen affinity purified polyclonal antibody (Catalog # PB9089) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp90 Alpha at approximately 95 kDa. The expected band size for Hsp90 Alpha is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-hsp90aa1-primary-antibodies-if-testing-7.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; IF analysis of Hsp90 alpha using anti-Hsp90 alpha antibody (PB9089). <br> Hsp90 alpha was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Hsp90 alpha Antibody (PB9089) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-hsp90aa1-primary-antibodies-fcm-testing-8.pngAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-Hsp90 alpha antibody (PB9089). <br>Overlay histogram showing A549 cells stained with PB9089 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp90 alpha Antibody (PB9089, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-fphar-15-1434812-g009.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg;Effect of BC on the expression of diverse proteins in BT549 cells. (A) Image of MAPK signaling pathway-related protein expression bands. (B) The expression of MAPK signaling pathway-related proteins after BC treatment of BT549 cells. (* P < 0.05, ** P < 0.01 versus control group). (C) Molecular docking diagram of BC and HSP90AA1 protein. (D) Molecular docking diagram of BC and PTGS21 protein. (E) Image of hub protein expression bands. (F) The expression of hub proteins after BC treatment of BT549 cells. (G) Images of HSP90AA1, PTGS2, and β-Actin protein expression bands of the cellular thermal shift assay. (H) Statistical results of HSP90AA1, PTGS2, and β-Actin protein expression bands of the cellular thermal shift assay. (* P < 0.05, ** P < 0.01 versus control group).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1434812/full'>39502536</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-fphar-15-1434812-g005.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg;HSP90AA1 and PTGS2 gene mutation and methylation in BRCA. (A, B) The alteration frequency, mutation types, mutation sites, and promoter methylation level of HSP90AA1 and PTGS2. (C) The relationship between SNV, CNV and HSP90AA1 expression, and the correlation between HSP90AA1 and the expression of 44 marker genes of RNA modification. (D) The relationship between SNV, CNV and PTGS2 expression, and the correlation between PTGS2 and the expression of 44 marker genes of RNA modification. (* P < 0.05, **** P < 0.0001 versus control group).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1434812/full'>39502536</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9089-fphar-15-1434812-g004.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg;Expression of hub genes in BCRA from TCGA and GTEx data. (A) HSP90AA1 was upregulated in BRCA. (B) The expression of HSP90AA1 was associated with the stage of BRCA. (C) The protein expression of HSP90AA1 in BRCA. (D) PTGS2 was downregulated in BRCA. (E) The expression of PTGS2 was associated with the stage of BRCA. (F) The protein expression of PTGS2. (* P < 0.05 and **** P < 0.0001 versus control group).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1434812/full'>39502536</a></b> https://www.bosterbio.com/picokine-elisa-kits/mouse-vegfr3-flt4-picokine-trade-elisa-kit-ek0546-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0546.pngMouse VEGFR3/FLT4 ELISA Kit PicoKine®Mouse VEGFR3/FLT4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-amphiregulin-ar-picokine-trade-elisa-kit-ek0591-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0591.pngMouse Amphiregulin(AR) ELISA Kit PicoKine®Mouse Amphiregulin/AR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il7r-cd127-picokine-trade-elisa-kit-ek0714-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0714.pngMouse IL7R/CD127 ELISA Kit PicoKine®Mouse IL7R/CD127 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-17e-il-25-picokine-trade-elisa-kit-ek0793-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0793.pngHuman IL-17E/IL-25 ELISA Kit PicoKine®Human IL-17E/IL-25 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-trem-1-picokine-trade-elisa-kit-ek0845-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0845.pngMouse TREM-1 ELISA Kit PicoKine®Mouse TREM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sparcl1-picokine-trade-elisa-kit-ek1309-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1309.jpgHuman SPARCL1/SPARC-like protein 1 ELISA Kit PicoKine®Human SPARCL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-fcgr2b-c-picokine-trade-elisa-kit-ek1313-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1313_1.pngMouse Fc gamma RII/RIII (CD32/CD16)(Fcgr2b/Fcgr3) ELISA Kit PicoKine®Mouse CD32/FCGR2b/c PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ve-cadherin-cd144-picokine-trade-elisa-kit-ek1318-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1318.jpgMouse VE-Cadherin Cdh5 ELISA Kit PicoKine®Mouse VE-Cadherin/CD144 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd93-c1qr-picokine-trade-elisa-kit-ek1320-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1320.jpgHuman CD93/C1qR ELISA Kit PicoKine®Human CD93/C1qR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-slam-cd150-picokine-trade-elisa-kit-ek1321-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1321.pngHuman SLAM/CD150 ELISA Kit PicoKine®Human SLAM/CD150 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dkk-3-picokine-trade-elisa-kit-ek1323-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1323_1.pngHuman DKK-3 ELISA Kit PicoKine®Human DKK-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-il-3-picokine-trade-elisa-kit-ek1324-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1324.jpgRat IL-3 ELISA Kit PicoKine®Rat IL-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-adiponectin-picokine-trade-elisa-kit-ek1327-boster.html2026-03-24T05:04:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1327.pngRat Adiponectin ELISA Kit PicoKine®Rat Adiponectin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-chemerin-rarres2-picokine-trade-elisa-kit-ek1329-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1329.jpgHuman Chemerin/RARRES2 ELISA Kit PicoKine®Human Chemerin/RARRES2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-chemerin-rarres2-picokine-trade-elisa-kit-ek1330-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1330.jpgMouse Chemerin/RARRES2 ELISA Kit PicoKine®Mouse Chemerin/RARRES2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-osteoactivin-gpnmb-picokine-trade-elisa-kit-ek1331-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1331.pngMouse GPNMB/Osteoactivin ELISA Kit PicoKine®Mouse Osteoactivin/GPNMB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-madcam-1-picokine-trade-elisa-kit-ek1332-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1332.jpgMouse MADCAM-1 ELISA Kit PicoKine®Mouse MADCAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-timp-4-picokine-trade-elisa-kit-ek1335-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1335.jpgMouse TIMP-4 ELISA Kit PicoKine®Mouse TIMP-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mesothelin-picokine-trade-elisa-kit-ek1338-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1338.pngHuman Mesothelin ELISA Kit PicoKine®Human Mesothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sdc1-syndecan-1-picokine-trade-elisa-kit-ek1339-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1339.jpgHuman SDC1 / Syndecan-1 / CD138 ELISA Kit PicoKine®Human SDC1/Syndecan-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adamts1-picokine-trade-elisa-kit-ek1340-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1340.jpgHuman ADAMTS1 ELISA Kit PicoKine®Human ADAMTS1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adam9-picokine-trade-elisa-kit-ek1341-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1341.pngHuman ADAM9 ELISA Kit PicoKine®Human ADAM9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-neuropilin-1-picokine-trade-elisa-kit-ek1342-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1342.jpgMouse Neuropilin-1 ELISA Kit PicoKine®Mouse Neuropilin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-neuropilin-1-picokine-trade-elisa-kit-ek1343-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1343.jpgRat Neuropilin-1 ELISA Kit PicoKine®Rat Neuropilin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-neuropilin-2-picokine-trade-elisa-kit-ek1344-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1344.pngMouse Neuropilin-2 ELISA Kit PicoKine®Mouse Neuropilin-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-neuropilin-2-picokine-trade-elisa-kit-ek1345-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1345.pngRat Neuropilin-2 ELISA Kit PicoKine®Rat Neuropilin-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-vegfr3-flt4-picokine-trade-elisa-kit-ek1347-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1347.pngRat VEGFR3/FLT4 ELISA Kit PicoKine®Rat VEGFR3/FLT4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-afp-picoband-trade-antibody-pb9090-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9090-afp-primary-antibodies-wb-testing-1.jpgAnti-alpha 1 Fetoprotein/AFP Antibody Picoband&reg; Western blot analysis of AFP using anti-AFP antibody (PB9090). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFP antigen affinity purified polyclonal antibody (Catalog # PB9090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFP at approximately 69 kDa. The expected band size for AFP is at 69 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9090-2_1.jpgAnti-alpha 1 Fetoprotein/AFP Antibody Picoband&reg; IHC analysis of AFP using anti-AFP antibody (PB9090).<br> AFP was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AFP Antibody (PB9090) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd3-epsilon-picoband-trade-antibody-pb9093-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-40820_2020_540_fig5_html.pngAnti-CD3 epsilon/CD3E Antibody Picoband&reg;a Schematic diagram of BCP-GNCs-mediated macrophage polarization and DC maturation by releasing IL-4 and DXMS. b IF staining of Arg1 (red) and iNOS (green) of macrophage, CD83 (red) and CD40 (green) of DC and nuclei (blue) after macrophages or DCs seeded on the BCP with 690 nm far-red or 808 nm NIR stimulating the release of PBS (BCP-PBS), 690 nm far-red stimulating the release of IL-4 (BCP-IL4) or 808 nm NIR stimulating the release of DXMS (BCP-DXMS) for 24 h. Scale bar = 5 μm. c, d Semiquantification of positively stained cells in ( b ). n = 5, ** P < 0.01, *** P < 0.001. e Flowchart of time course for irradiating BCP-GNC in vivo. f–h IHC staining of M1, M2 macrophages (iNOS and Arg1), mature DCs (CD40), T cells (CD3), MSCs and osteoblasts (CD146, Runx2) under the BCP-GNCs implant in vivo. Scale bar = 100 μm. Red arrow, positive cells. M, material. i – k Semiquantification of positively stained cells in (E, F, G). n = 5, * P < 0.05, ** P < 0.01, **** P < 0.0001. l H&E and Masson staining of implant area of BCP-Con and BCP-GNCs in vivo after 4 weeks (4 W) (the red dash line shows the new bone formation area; NB, new bone; M, material). m Semiquantification of new bone area in ( k ). n = 5, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s40820-020-00540-z'>34138183</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-40820_2020_540_fig6_html.pngAnti-CD3 epsilon/CD3E Antibody Picoband&reg;a Implementation strategy of clodronate on macrophages depletion. b H&E and Masson staining of BCP implant area treating with PBS or clodronate in vivo after implant 4 weeks (the red dash line shows the new bone formation area; NB, new bone; M, material). Scale bar = 100 μm. c IHC staining of M2 macrophages (Arg1) and osteoblasts (Runx2, Col1a1) under the BCP implant treating with PBS or clodronate in vivo. Scale bar = 100 μm. Red arrow, positive cells. M, material. d – g Semiquantification of new bone area and positively stained cells in ( b, c ). n = 5, *** P < 0.001, **** P < 0.0001. h Implementation strategy of diphtheria toxin (DT) on DCs depletion. i H&E and Masson staining of BCP implant area treating with PBS or DT in vivo after implant 4 weeks (the red dash line shows the new bone formation area; NB, new bone; M, material). Scale bar = 100 μm. j IHC staining of T cells (CD3) and osteoblasts (Runx2, Col1a1) under the BCP implant treating with PBS or DT in vivo. Scale bar = 100 μm. Red arrow, positive cells. M, material. k – n Semiquantification of new bone area and positively stained cells in ( i, j ). n = 5, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1007/s40820-020-00540-z'>34138183</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-41467_2022_32104_fig2_html.pngAnti-CD3 epsilon/CD3E Antibody Picoband&reg;OSBP translocates from Golgi to PM via interacting with ORP4L. a Electron micrographs of ORP4L KI and wild-type T-cells, HepG2 and 293 T cells. Scale bar, 100 nm. Black triangles present in dividual data points for n = 20 cells from one or cell line. The central mark indicates the median, the bottom and top edges of the box indicate the interquartile range, and the whiskers represent the maximum and minimum point. One-way ANOVA test with a confidence interval of 95% was used to compute statistics. b Co-immunoprecipitation analysis of ORP4L binding to OSBP in ORP4L KI T-cells with or without anti-CD3 stimulation. c The localization of OSBP in ORP4L KI T-cells. Scale bar, 10 μm. d The localization of OSBP in ORP4L KI and wild-type T-cells with or without anti-CD3 stimulation. Scale bar, 10 μm. e Western blot analysis of OSBP protein levels in PM and Golgi of ORP4L KI or wild-type T-cells. f A schematic representation describing the BiFC technique. g Interactions between ORP4L/pVn-C1 and OSBP/pVc-C1 in ORP4L KI T-cells as determined by BiFC. Scar bar, 10 μm. h The localization of OSBP in ORP4L KI T-cells upon ORP4L knockdown. Scar bar, 10 μm. i Western blot analysis of OSBP protein levels in PM and Golgi of ORP4L KI T-cells upon ORP4L knockdown. j The localization of overexpressed wild-type OSBP or OSBP ( △ ORP4L) in ORP4L KI T-cells. Scar bar, 10 μm. k Western blot analysis of OSBP protein levels in PM and Golgi of ORP4L KI T-cells with or without VAPA knockdown. l Western blot analysis of overexpressed wild-type OSBP or OSBP( △ FFAT) protein levels in the PM and Golgi of ORP4L KI T-cells. Confocal microscopy and blot images are representative of n = 3 biological replicates with similar results. The same experiments were repeated twice in T-cells from two mice. In ( c , d , h and j ), the ration of OSBP fluorescence density from n = 10 cells from one between PM and Golgi are shown below. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-32104-7'>35906240</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-41467_2022_32104_fig4_html.pngAnti-CD3 epsilon/CD3E Antibody Picoband&reg;Pathological findings of T-cell leukemia in ORP4L KI mice. a Kaplan–Meier comparative survival analysis of ORP4L KI and WT littermate mice ( n = 20 mice per group, Log-rank test). b White blood cell count on peripheral blood of ORP4L KI and WT littermate mice. Black triangles present in dividual data points for n = 15 (ORP4L KI) and n = 12 (WT) mice. c The percentage of indicated cells in peripheral blood of ORP4L KI and WT littermate mice. Black triangles present in dividual data points for n = 10 mice. d Percentage of CD25 + Foxp3 + or CD25 + CCR4 + cells in peripheral blood of ORP4L KI and WT littermate mice. Black triangles present in dividual data points for n = 8 (ORP4L KI) and n = 6 (WT) mice. e Flow cytometry analysis of Foxp3 and CCR4 expression in ORP4L KI and WT littermate mice. The same experiments were repeated twice in two mice. f Representative blood smears of ORP4L KI and WT littermate mice ( n = 3 mice). Scale bars, 10 μm. g , h Splenomegaly and hepatomegaly in ORP4L KI mice. The right panels illustrate the organ weights. Black triangles present in dividual data points for n = 10 (ORP4L KI) and n = 6 (WT) mice. Scale bars, 1 cm. i Representative H&E-staining of organs in ORP4L KI mice. Immunohistochemical anti-CD3 antibody staining (inserts) of the tissues is shown. Scale bars, 100 μm. j Gross and histological findings of splenomegaly in B-NDG mice after transplantation of spleen cells from ORP4L KI mice. Black triangles present in dividual data points for n = 6 mice. Scale bars, 1 cm. k Representative H&E and anti-CD3 antibody staining of spleen of j . Scale bars, 100 μm. Images of i and k are representative of n = 3 biological replicates with similar results. The same experiments were repeated twice in two mice. In each box plot, the central mark indicates the median, the bottom and top edges of the box indicate the interquartile range, and the whiskers represent the maximum and minimum point. Two-tailed unpaired t -test with a confidence interval of 95% was used to compute statistics. P -values are indicated in the figures. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-022-32104-7'>35906240</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-ccid-16-639-g0003.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg;Subcutaneous injection of recombinant IL-33 aggravated the pathological changes of HFs and T cells infiltration in IMQ-induced psoriatic mice. ( a ) Schematic of the treatment performed. ( b ) H & E staining (n = 4 per group), PBS vs IL-33. Scale bar=100μm. (i) infundibulum, (ii) outer root sheath. ( c ) Immunohistochemical staining for CD3 (n=4 per group), PBS vs IL-33. Red arrows indicate CD3-positive T cells. Scale bar=100μm. ( d and e ) Statistical data of HFs number, HFs length, HFs diameter, sebaceous gland number and CD3-positive cells. Data are representative of three independent experiments. Significance was determined by a two-tailed Student’s t -test. * P <0.05, ** P <0.01, *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10019523/&orig_host=www.ncbi.nlm.nih.gov'>36936754</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-ccid-16-639-g0002.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg;The pathological changes of HFs and T cells infiltration were alleviated in IL-33 −/− and ST2 −/− mice in IMQ-induced psoriatic model. ( a ) H & E staining of lesion skin in WT psoriatic mice and IL-33 −/− psoriatic mice (n = 6 per group). Scale bar=100μm. (i) infundibulum, (ii) outer root sheath, (iii) sebaceous glands. ( b ) Immunohistochemical staining of CD3 in WT psoriatic mice and IL-33 −/− psoriatic mice (n = 6 per group). Scale bar = 100μm. Red arrows indicate CD3-positive T cells. ( c and d ) Statistical data of HFs number, HFs length, HFs diameter, sebaceous gland number and CD3-positive cells. ( e ) H & E staining of lesion skin in WT psoriatic mice and ST2 −/− psoriatic mice (n = 6 per group). Scale bar=100μm. (i) infundibulum, (ii) outer root sheath, (iii) sebaceous glands. ( f ) Immunohistochemical staining of CD3 in WT psoriatic mice and ST2 −/− psoriatic mice (n = 6 per group). Scale bar = 100μm. Red arrows indicate CD3-positive T cells. ( g and h ) Statistical data of HFs number, HFs length, HFs diameter, sebaceous gland number and CD3-positive cells. Data are representative of three independent experiments. Significance was determined by a two-tailed Student’s t -test. * P <0.05, ** P <0.01, *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10019523/&orig_host=www.ncbi.nlm.nih.gov'>36936754</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-ccid-16-639-g0001.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg;The pathological changes of HFs in IMQ-induced psoriatic mice were similar to those seen in psoriatic patients. ( a ) H & E staining of the normal skin and psoriatic skin. Scale bar = 100μm. (i) infundibulum, (ii) outer root sheath, (iii) sebaceous glands. ( b ) Immunohistochemical staining of IL-33 in normal skin and psoriatic skin. Scale bar=100μm. ( c ) Immunohistochemical staining of CD3 in normal skin and psoriatic skin. Scale bar = 100μm. ( d ) Statistical data of HFs diameter, IL-33-positive cells and CD3-positive cells. ( e ) H & E staining of the normal skin and IMQ-induced psoriatic skin. Scale bar=100μm. (i) infundibulum, (ii) outer root sheath, (iii) sebaceous glands. ( f ) Immunohistochemical staining of IL-33 in normal skin and IMQ-induced psoriatic skin. Scale bar=100μm. ( g ) Immunohistochemical staining of CD3 in normal skin and IMQ-induced psoriatic skin. Scale bar=100μm. Red arrows indicate CD3-positive T cells. ( h ) Statistical data of HFs number, HFs length, HFs diameter, IL-33-positive cells and CD3-positive cells. Data are representative of three independent experiments. Significance was determined by a two-tailed Student’s t -test. * P <0.05, ** P <0.01, *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10019523/&orig_host=www.ncbi.nlm.nih.gov'>36936754</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-cd3e-primary-antibodies-wb-testing-2_1.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg;Western blot analysis of CD3E using anti-CD3E antibody (PB9093). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human Hut-78 whole cell lysates,<br> Lane 4: human Hut-78 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E antigen affinity purified polyclonal antibody (Catalog # PB9093) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3E at approximately 23 kDa. The expected band size for CD3E is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-2-ihc-anti-cd3-epsilon-picoband-antibody.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg; IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody (PB9093). <br> CD3 Epsilon was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD3 Epsilon Antibody (PB9093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-3-ihc-anti-cd3-epsilon-picoband-antibody.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg; IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody (PB9093). <br> CD3 Epsilon was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD3 Epsilon Antibody (PB9093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-4-ihc-anti-cd3-epsilon-picoband-antibody.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg; IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody (PB9093). <br> CD3 Epsilon was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD3 Epsilon Antibody (PB9093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-5-ihc-anti-cd3-epsilon-picoband-antibody.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg; IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody (PB9093).<br> CD3 Epsilon was detected in frozen section of rat spleen tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD3 Epsilon Antibody (PB9093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-6-ihc-anti-cd3-epsilon-picoband-antibody.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg; IHC analysis of CD3 Epsilon using anti-CD3 Epsilon antibody (PB9093).<br> CD3 Epsilon was detected in frozen section of mouse spleen tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD3 Epsilon Antibody (PB9093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-yanhua_li.pngAnti-CD3 epsilon/CD3E Antibody Picoband&reg;IF analysis of CD3E using anti-CD3E antibody (PB9093). <br> CD3E was detected in a paraffin-embedded section of mouse 4T1 cell xenograft tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-CD3E Antibody (PB9093) overnight at 4°C. DyLight 550-conjugated Goat Anti-Rabbit was used as secondary antibody incubated for 1 hour at RT. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-7_1.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg; IF analysis of CD3E and CD68 using anti-CD3E antibody (PB9093) and anti-CD68 antibody (M00602) <br> CD3E and CD68 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD3E Antibody (PB9093) and mouse anti CD68 Antibody(M00602) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) and Biotin conjugated goat anti-rabbit IgG (BA1003) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9093-cd3e-primary-antibodies-if-testing-8.jpgAnti-CD3 epsilon/CD3E Antibody Picoband&reg; IF analysis of CD3E and CD20 using anti-CD3E antibody (PB9093) and anti-CD20 antibody (M03780-5).<br> CD3E and CD20 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-CD3E antibody (PB9093) and mouse anti-CD20 antibody (M03780-5) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135), DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-app-picoband-trade-antibody-pb9091-boster.html2026-03-25T05:21:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9091-APP-primary-antibodies-WB-testing-1.jpgAnti-beta Amyloid/APP Antibody Picoband&reg; Western blot analysis of APP using anti-APP antibody (PB9091). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: human Hela whole cell lysates&#44;<br>Lane 2: human U-87MG whole cell lysates&#44;<br>Lane 3: human T-47D whole cell lysates&#44;<br>Lane 4: human A549 whole cell lysates&#44;<br>Lane 5: human U2OS whole cell lysates&#44;<br>Lane 6: rat brain tissue lysates&#44;<br>Lane 7: mouse brain tissue lysates. <br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APP antigen affinity purified polyclonal antibody (Catalog # PB9091) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APP at approximately 120KD. The expected band size for APP is at 87KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9091-APP-primary-antibodies-IHC-testing-2.jpgAnti-beta Amyloid/APP Antibody Picoband&reg; IHC analysis of APP using anti-APP antibody (PB9091).<br>APP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APP Antibody (PB9091) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9091-APP-primary-antibodies-IHC-testing-3.jpgAnti-beta Amyloid/APP Antibody Picoband&reg; IHC analysis of APP using anti-APP antibody (PB9091).<br>APP was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APP Antibody (PB9091) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9091-APP-primary-antibodies-IHC-testing-4.jpgAnti-beta Amyloid/APP Antibody Picoband&reg; IHC analysis of APP using anti-APP antibody (PB9091).<br>APP was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APP Antibody (PB9091) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9091-APP-primary-antibodies-IHC-testing-5.jpgAnti-beta Amyloid/APP Antibody Picoband&reg; IHC analysis of APP using anti-APP antibody (PB9091).<br>APP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APP Antibody (PB9091) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9091-APP-primary-antibodies-IHC-testing-6.jpgAnti-beta Amyloid/APP Antibody Picoband&reg; IHC analysis of APP using anti-APP antibody (PB9091).<br>APP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APP Antibody (PB9091) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9091-APP-primary-antibodies-IF-testing-7.jpgAnti-beta Amyloid/APP Antibody Picoband&reg; IF analysis of APP using anti-APP antibody (PB9091). <br> APP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-APP Antibody (PB9091) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-myc-picoband-trade-antibody-pb9092-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9092-myc-primary-antibodies-wb-testing-1.jpgAnti-c-Myc Antibody Picoband&reg; Western blot analysis of c-Myc using anti-c-Myc antibody (PB9092). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Myc antigen affinity purified polyclonal antibody (Catalog # PB9092) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Myc at approximately 60-65 kDa. The expected band size for c-Myc is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9092-myc-primary-antibodies-if-testing-2.jpgAnti-c-Myc Antibody Picoband&reg; IF analysis of c-Myc using anti-c-Myc antibody (PB9092) and anti-Beta Tubulin antibody (M01857-3).<br> c-Myc was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-c-Myc Antibody (PB9092) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd31-picoband-trade-antibody-pb9094-boster.html2026-03-24T05:04:35+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9094-cd31-primary-antibodies-wb-testing-1_1.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human THP-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 90 kDa, 130 kDa. The expected band size for CD31/PECAM1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9094-cd31-primary-antibodies-ihc-testing-2_1.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PB9094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9094-cd31-primary-antibodies-ihc-testing-3_1.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094). <br> CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PB9094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9094-cd31-primary-antibodies-fcm-testing-4.jpgAnti-CD31/PECAM1 Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-CD31/PECAM1 antibody (PB9094). <br> Overlay histogram showing Jurkat cells stained with PB9094 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD31/PECAM1 Antibody (PB9094, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd43-picoband-trade-antibody-pb9095-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9095-1_1.jpgAnti-CD43/SPN Antibody Picoband&reg; Western blot analysis of CD43 using anti-CD43 antibody (PB9095). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD43 antigen affinity purified polyclonal antibody (Catalog # PB9095) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD43 at approximately 115KD. The expected band size for CD43 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9095-2-IHC-anti-cd43-picoband-antibody.jpgAnti-CD43/SPN Antibody Picoband&reg; IHC analysis of CD43 using anti-CD43 antibody (PB9095). <br> CD43 was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD43 Antibody (PB9095) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9095-3-IHC-anti-cd43-picoband-antibody.jpgAnti-CD43/SPN Antibody Picoband&reg; IHC analysis of CD43 using anti-CD43 antibody (PB9095). <br> CD43 was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD43 Antibody (PB9095) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9095-4-IHC-anti-cd43-picoband-antibody.jpgAnti-CD43/SPN Antibody Picoband&reg; IHC analysis of CD43 using anti-CD43 antibody (PB9095). <br> CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD43 Antibody (PB9095) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9095-5-IF-anti-cd43-picoband-antibody.jpgAnti-CD43/SPN Antibody Picoband&reg; IHC analysis of CD43 using anti-CD43 antibody (PB9095).<br> CD43 was detected in immunocytochemical section of K562 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CD43 Antibody (PB9095) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9095-6_1.jpgAnti-CD43/SPN Antibody Picoband&reg; IF analysis of CD43 using anti-CD43 antibody (PB9095) <br> CD43 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD43 Antibody (PB9095) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9095-7.pngAnti-CD43/SPN Antibody Picoband&reg; Flow Cytometry analysis of P3NSI cells using anti-CD43 antibody (PB9095). <br> Overlay histogram showing P3NSI cells stained with PB9095 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (PB9095&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9095-8.pngAnti-CD43/SPN Antibody Picoband&reg; Flow Cytometry analysis of BRL cells using anti-CD43 antibody (PB9095). <br> Overlay histogram showing BRL cells stained with PB9095 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (PB9095&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9095-9.pngAnti-CD43/SPN Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-CD43 antibody (PB9095). <br> Overlay histogram showing HL-60 cells stained with PB9095 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD43 Antibody (PB9095&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd45-picoband-trade-antibody-pb9096-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9096-fendo-09-00476-g005.jpgAnti-CD45/PTPRC Antibody Picoband&reg;Effects of long-term L -serine administration on orexigenic neuropeptide expression in aging mice. NPY (A–E) and AGRP (F–J) expression as determined by immunohistochemistry; relative NPY (K) and AGRP (L) mRNA expression. Aged, untreated control mice; Low-ser, mice supplemented with 0.1% (wt/vol) L -serine dissolved in the drinking water; Middle-ser, mice supplemented with 0.2% (wt/vol) L -serine dissolved in the drinking water; High-ser, mice supplemented with 0.5% (wt/vol) L -serine dissolved in the drinking water. Young, adult male mice at the age of 18 months. Arrow (yellow), expression of target proteins; NPY, neuropeptide Y; AGRP, agouti-related protein. Values are expressed as mean ± SEM, n = 8; a, b Means of the bars with different letters were significantly different among groups ( P < 0.05).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2018.00476/full'>30190704</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9096-cd45-primary-antibodies-wb-testing-1.jpgAnti-CD45/PTPRC Antibody Picoband&reg; Western blot analysis of CD45 using anti-CD45 antibody (PB9096). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45 antigen affinity purified polyclonal antibody (Catalog # PB9096) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45 at approximately 180-250 kDa. The expected band size for AFP is at 147 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9096-5.pngAnti-CD45/PTPRC Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-CD45 antibody (PB9096). <br> Overlay histogram showing THP-1 cells stained with PB9096 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD45 Antibody (PB9096&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9096-2-ihc-anti-cd45-picoband-antibody.jpgAnti-CD45/PTPRC Antibody Picoband&reg; IHC analysis of CD45 using anti-CD45 antibody (PB9096). <br> CD45 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD45 Antibody (PB9096) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9096-3_1.jpgAnti-CD45/PTPRC Antibody Picoband&reg; IF analysis of CD45 using anti-CD45 antibody (PB9096) <br> CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD45 Antibody (PB9096) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9096-4.jpgAnti-CD45/PTPRC Antibody Picoband&reg; IF analysis of CD45 using anti-CD45 antibody (PB9096) <br> CD45 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD45 Antibody (PB9096) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-iii-antibody-rp1033-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9098-2-RP1033-WB-anti-collagen-iii-antibody.jpgAnti-Collagen III/COL3A1 Antibody Picoband&reg;Anti-Collagen III Picoband antibody&#44; RP1033-2.jpg<br>All lanes: Anti Collagen III(RP1033) at 0.5ug/ml<br>Lane 1: MCF-4 Whole Cell Lysate at 40ug<br>Lane 2: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 139KD<br>Observed bind size: 139KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1033-1-WB-anti-collagen-iii-antibody.jpgAnti-Collagen III/COL3A1 Antibody Picoband&reg;Anti-Collagen III Picoband antibody&#44; RP1033-1.jpg<br>All lanes: Anti Collagen III (RP1033) at 0.5ug/ml<br>WB: Recombinant Human Collagen III Protein 0.5ng<br>Predicted bind size: 39KD<br>Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-iv-picoband-trade-antibody-pb9099-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9099-1_1.jpgAnti-Collagen IV/COL4A1 Antibody Picoband&reg; Western blot analysis of Collagen IV using anti-Collagen IV antibody (PB9099). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen IV antigen affinity purified polyclonal antibody (Catalog # PB9099) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen IV at approximately 250KD. The expected band size for Collagen IV is at 161KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9099-2-IHC-anti-collagen-iv-picoband-antibody.jpgAnti-Collagen IV/COL4A1 Antibody Picoband&reg; IHC analysis of Collagen using anti-Collagen antibody (PB9099). <br> Collagen was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen Antibody (PB9099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9099-3.jpgAnti-Collagen IV/COL4A1 Antibody Picoband&reg; IF analysis of COL4A1 using anti-COL4A1 antibody (PB9099) <br> COL4A1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9099-4.jpgAnti-Collagen IV/COL4A1 Antibody Picoband&reg; IF analysis of COL4A1/E Cadherin using anti-COL4A1/E Cadherin antibody (PB9099/M00063-2) <br> COL4A1/E Cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-COL4A1 Antibody (PB9099)/mouse anti E Cadherin Antibody(M00063-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) /Cy3 conjugated Goat anti mouse IgG(BA1031)&#44;were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-creb-picoband-trade-antibody-pb9100-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9100-creb1-primary-antibodies-wb-testing-1.jpgAnti-CREB/CREB1 Antibody Picoband&reg;Western blot analysis of CREB1 using anti-CREB1 antibody (PB9100). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 antigen affinity purified polyclonal antibody (PB9100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CREB1 at approximately 43 kDa. The expected band size for CREB1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9100-creb1-primary-antibodies-fcm-testing-1.jpgAnti-CREB/CREB1 Antibody Picoband&reg;Flow Cytometry analysis of 293T cells using anti-CREB1 antibody (PB9100). <br> Overlay histogram showing 293T cells stained with PB9100 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CREB1 Antibody (PB9100, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-crk-p38-picoband-trade-antibody-pb9101-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9101-crk-primary-antibodies-wb-testing-1.jpgAnti-Crk p38 Antibody Picoband&reg; Western blot analysis of Crk p38 using anti-Crk p38 antibody (PB9101). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Crk p38 antigen affinity purified polyclonal antibody (Catalog # PB9101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Crk p38 at approximately 38 kDa. The expected band size for Crk p38 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9101-crk-primary-antibodies-ihc-testing-2.jpgAnti-Crk p38 Antibody Picoband&reg; IHC analysis of Crk p38 using anti-Crk p38 antibody (PB9101). <br> Crk p38 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Crk p38 Antibody (PB9101) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9101-crk-primary-antibodies-ihc-testing-3.jpgAnti-Crk p38 Antibody Picoband&reg; IHC analysis of Crk p38 using anti-Crk p38 antibody (PB9101). <br> Crk p38 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Crk p38 Antibody (PB9101) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9101-crk-primary-antibodies-ihc-testing-4.jpgAnti-Crk p38 Antibody Picoband&reg; IHC analysis of Crk p38 using anti-Crk p38 antibody (PB9101). <br> Crk p38 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Crk p38 Antibody (PB9101) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-csk-picoband-trade-antibody-pb9102-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9102-1-WB-anti-csk-picoband-antibody.jpgAnti-Csk Antibody Picoband&reg; Western blot analysis of CSK using anti-CSK antibody (PB9102). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant human CSK protein 0.5 ng <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSK antigen affinity purified polyclonal antibody (Catalog # PB9102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSK at approximately 47 kDa. The expected band size for CSK is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9102-2-WB-anti-csk-picoband-antibody.jpgAnti-Csk Antibody Picoband&reg; Western blot analysis of CSK using anti-CSK antibody (PB9102). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates, <br> Lane 2: rat thymus tissue lysates, <br> Lane 3: mouse liver tissue lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human Jurkat whole cell lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: human MCF-7 whole cell lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates, <br> Lane 9: mouse NEURO whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSK antigen affinity purified polyclonal antibody (Catalog # PB9102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSK at approximately 51 kDa. The expected band size for CSK is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9102-3.jpgAnti-Csk Antibody Picoband&reg; IHC analysis of CSK using anti-CSK antibody (PB9102). CSK was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CSK Antibody (PB9102) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9102-4.jpgAnti-Csk Antibody Picoband&reg; IHC analysis of Csk using anti-Csk antibody (PB9102). <br> Csk was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Csk Antibody (PB9102) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-d3-picoband-trade-antibody-pb9103-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9103-1-WB-anti-cyclin-d3-picoband-antibody.jpgAnti-Cyclin D3/CCND3 Antibody Picoband&reg; Western blot analysis of Cyclin D3 using anti-Cyclin D3 antibody (PB9103). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> lane 1: recombinant human Cyclin D3 protein 0.5ng.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D3 antigen affinity purified polyclonal antibody (Catalog # PB9103) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D3 at approximately 25KD. The expected band size for Cyclin D3 is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9103-2-WB-anti-cyclin-d3-picoband-antibody.jpgAnti-Cyclin D3/CCND3 Antibody Picoband&reg; Western blot analysis of Cyclin D4 using anti-Cyclin D4 antibody (PB9103). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> lane 1: rat testis tissue lysate&#44;<br> lane 2: rat thymus tissue lysate&#44;<br> lane 3: rat lung tissue lysate&#44;<br> lane 4: rat ovary tissue lysate&#44;<br> lane 5: JURKAT whole cell lysate&#44;<br> lane 6: A549 whole cell lysate&#44;<br> lane 7: MCF-7 whole cell lysate&#44;<br> lane 8: HELA whole cell lysate.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D4 antigen affinity purified polyclonal antibody (Catalog # PB9103) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D4 at approximately 33KD. The expected band size for Cyclin D4 is at 33KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-b1-picoband-trade-antibody-pb9104-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9104-ccnb1-primary-antibodies-wb-testing-1.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband&reg; Western blot analysis of CCNB1 using anti-CCNB1 antibody (PB9104). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> lane 1: HELA whole cell lysate,<br> lane 2: 293T whole cell lysate,<br> lane 3: MCF-7 whole cell lysate,<br> lane 4: COLO320 whole cell lysate.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # PB9104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 56KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9104-ccnb1-primary-antibodies-fc-testing-3.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-CCNB1 antibody (PB9104).<br>Overlay histogram showing THP-1 cells stained with PB9104 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCNB1 Antibody (PB9104&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9104-ccnb1-primary-antibodies-if-testing-2.jpgAnti-Cyclin B1/CCNB1 Antibody Picoband&reg; IF analysis of CCNB1 using anti-CCNB1 antibody (PB9104). <br> CCNB1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCNB1 Antibody (PB9104) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-desmin-picoband-trade-antibody-pb9105-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-wb-testing-1.jpgAnti-Desmin Antibody Picoband&reg; Western blot analysis of Desmin using anti-Desmin antibody (PB9105). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat skeletal muscle tissue lysates,<br> Lane 3: mouse C2C12 whole cell lysates,<br> Lane 4: mouse heart tissue lysates,<br> Lane 5: mouse skeletal muscle tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Desmin antigen affinity purified polyclonal antibody (Catalog # PB9105) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Desmin at approximately 54 kDa. The expected band size for Desmin is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-ihc-testing-2.jpgAnti-Desmin Antibody Picoband&reg; IHC analysis of Desmin using anti-Desmin antibody (PB9105). <br> Desmin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Desmin Antibody (PB9105) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-ihc-testing-3.jpgAnti-Desmin Antibody Picoband&reg; IHC analysis of Desmin using anti-Desmin antibody (PB9105). <br> Desmin was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Desmin Antibody (PB9105) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-ihc-testing-4.jpgAnti-Desmin Antibody Picoband&reg; IHC analysis of Desmin using anti-Desmin antibody (PB9105). <br> Desmin was detected in a frozen section of rat cardiac muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Desmin Antibody (PB9105) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-if-testing-5.jpgAnti-Desmin Antibody Picoband&reg; IF analysis of Desmin using anti-Desmin antibody (PB9105). <br> Desmin was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Desmin Antibody (PB9105) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-if-testing-6.jpgAnti-Desmin Antibody Picoband&reg; IF analysis of Desmin using anti-Desmin antibody (PB9105). <br> Desmin was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Desmin Antibody (PB9105) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-if-testing-7.jpgAnti-Desmin Antibody Picoband&reg; IF analysis of Desmin using anti-Desmin antibody (PB9105). <br> Desmin was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Desmin Antibody (PB9105) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9105-desmin-primary-antibodies-if-testing-8_1.jpgAnti-Desmin Antibody Picoband&reg; IF analysis of Desmin using anti-Desmin antibody (PB9105). <br> Desmin was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Desmin Antibody (PB9105) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fsh-beta-picoband-trade-antibody-pb9106-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9106-1-WB-anti-fsh-beta-picoband-antibody.jpgAnti-FSH beta/FSHB Antibody Picoband&reg; Western blot analysis of FSH beta using anti-FSH beta antibody (PB9106). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours <br> Lane 1: recombinant human FSH beta protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH beta antigen affinity purified polyclonal antibody (Catalog # PB9106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH beta at approximately 36 kDa. The expected band size for FSH beta is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9106-2-IHC-anti-fsh-beta-picoband-antibody.jpgAnti-FSH beta/FSHB Antibody Picoband&reg; IHC analysis of FSH beta using anti-FSH beta antibody (PB9106). <br> FSH beta was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSH beta Antibody (PB9106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9106-3_1-IHC-anti-fsh-beta-picoband-antibody.jpgAnti-FSH beta/FSHB Antibody Picoband&reg; IHC analysis of FSH beta using anti-FSH beta antibody (PB9106). <br> FSH beta was detected in a paraffin-embedded section of human mammry cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FSH beta Antibody (PB9106) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glut4-picoband-trade-antibody-pb9109-boster.html2026-03-25T05:21:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9109-2-WB-anti-glut4-picoband-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg; Western blot analysis of GLUT4 using anti-GLUT4 antibody (PB9109). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> lane 1: rat cardiac muscle tissue lysate&#44;<br> lane 2: rat skeletal muscle tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT4 antigen affinity purified polyclonal antibody (Catalog # PB9109) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT4 at approximately 55KD. The expected band size for GLUT4 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9109-3-IHC-anti-glut4-picoband-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg; IHC analysis of GLUT4 using anti-GLUT4 antibody (PB9109). <br> GLUT4 was detected in frozen section of rat cardiac muscle tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PB9109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9109-4-IHC-anti-glut4-picoband-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg; IHC analysis of GLUT4 using anti-GLUT4 antibody (PB9109). <br> GLUT4 was detected in frozen section of mouse cardiac muscle tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PB9109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9109-5-IHC-anti-glut4-picoband-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg; IHC analysis of GLUT4 using anti-GLUT4 antibody (PB9109). <br> GLUT4 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PB9109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9109-6-IHC-anti-glut4-picoband-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg; IHC analysis of GLUT4 using anti-GLUT4 antibody (PB9109). <br> GLUT4 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PB9109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9109-7-IHC-anti-glut4-picoband-antibody.jpgAnti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband&reg; IHC analysis of GLUT4 using anti-GLUT4 antibody (PB9109). <br> GLUT4 was detected in paraffin-embedded section of mouse skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT4 Antibody (PB9109) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikk-alpha-picoband-trade-antibody-pb9110-boster.html2026-03-24T05:04:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9110-ikk-alpha-primary-antibodies-wb-testing-1.jpgAnti-IKK alpha/CHUK Antibody Picoband&reg; Western blot analysis of IKK alpha using anti-IKK alpha antibody (PB9110). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human Caco-2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKK alpha antigen affinity purified polyclonal antibody (Catalog # PB9110) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKK alpha at approximately 85 kDa. The expected band size for IKK alpha is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9110-ikk-alpha-primary-antibodies-ihc-testing-2.jpgAnti-IKK alpha/CHUK Antibody Picoband&reg; IHC analysis of IKKA using anti-IKKA antibody (PB9110). <br> IKKA was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IKKA Antibody (PB9110) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kv2-1-picoband-trade-antibody-pb9111-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9111-kcnb1-primary-antibodies-ihc-testing-7.jpgAnti-Kv2.1/KCNB1 Antibody Picoband&reg;IHC analysis of KCNB1 using anti-KCNB1 antibody (PB9111). <br>KCNB1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KCNB1 Antibody (PB9111) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9111-kv2.1-primary-antibodies-wb-testing-1_1.jpgAnti-Kv2.1/KCNB1 Antibody Picoband&reg; Western blot analysis of Kv2.1 using anti-Kv2.1 antibody (PB9111). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv2.1 antigen affinity purified polyclonal antibody (Catalog # PB9111) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv2.1 at approximately 96 kDa. The expected band size for Kv2.1 is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9111-2-ihc-anti-kv2-1-picoband-antibody_1.jpgAnti-Kv2.1/KCNB1 Antibody Picoband&reg; IHC analysis of KV2.1 using anti-KV2.1 antibody (PB9111). <br> KV2.1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KV2.1 Antibody (PB9111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9111-3-ihc-anti-kv2-1-picoband-antibody.jpgAnti-Kv2.1/KCNB1 Antibody Picoband&reg; IHC analysis of KV2.1 using anti-KV2.1 antibody (PB9111). <br> KV2.1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KV2.1 Antibody (PB9111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9111-4-ihc-anti-kv2-1-picoband-antibody.jpgAnti-Kv2.1/KCNB1 Antibody Picoband&reg; IHC analysis of KV2.1 using anti-KV2.1 antibody (PB9111).<br> KV2.1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KV2.1 Antibody (PB9111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9111-5-ihc-anti-kv2-1-picoband-antibody.jpgAnti-Kv2.1/KCNB1 Antibody Picoband&reg; IHC analysis of KV2.1 using anti-KV2.1 antibody (PB9111). <br> KV2.1 was detected in frozen section of rat brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KV2.1 Antibody (PB9111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9111-6-ihc-anti-kv2-1-picoband-antibody.jpgAnti-Kv2.1/KCNB1 Antibody Picoband&reg; IHC analysis of KV2.1 using anti-KV2.1 antibody (PB9111). <br> KV2.1 was detected in frozen section of mouse brain tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KV2.1 Antibody (PB9111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cias1-nalp3-picoband-trade-antibody-pb9114-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9114-1-WB-anti-cias1-nalp3-picoband-antibody.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg; Western blot analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody (PB9114). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human CIAS1 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PB9114) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIAS1/NALP3 at approximately 37 kDa. The expected band size for CIAS1/NALP3 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9114-2-WB-anti-cias1-nalp3-picoband-antibody.jpgAnti-CIAS1/NALP3/NLRP3 Antibody Picoband&reg; Western blot analysis of CIAS1/NALP3 using anti-CIAS1/NALP3 antibody (PB9114). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIAS1/NALP3 antigen affinity purified polyclonal antibody (Catalog # PB9114) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIAS1/NALP3 at approximately 118 kDa. The expected band size for CIAS1/NALP3 is at 118 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p-glycoprotein-antibody-rp1034-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9115-1-RP1034-IHC-anti-p-glycoprotein-antibody.jpgAnti-P Glycoprotein/ABCB1 AntibodyAnti-P Glycoprotein Picoband antibody&#44; RP1034-1.JPG<br>IHC(F): Mouse Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9115-2-RP1034-IHC-anti-p-glycoprotein-antibody.jpgAnti-P Glycoprotein/ABCB1 AntibodyAnti-P Glycoprotein Picoband antibody&#44; RP1034-2.JPG<br>IHC(F): Rat Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9115-3-RP1034-IHC-anti-p-glycoprotein-antibody.jpgAnti-P Glycoprotein/ABCB1 AntibodyAnti-P Glycoprotein Picoband antibody&#44; RP1034-3.JPG<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9115-4-RP1034-IHC-anti-p-glycoprotein-antibody.jpgAnti-P Glycoprotein/ABCB1 AntibodyAnti-P Glycoprotein Picoband antibody&#44; RP1034-4.JPG<br>IHC(P): Mouse Kidney Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9115-5-RP1034-IHC-anti-p-glycoprotein-antibody.jpgAnti-P Glycoprotein/ABCB1 AntibodyAnti-P Glycoprotein Picoband antibody&#44; RP1034-5.JPG<br>IHC(P): Rat Kidney Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rip-picoband-trade-antibody-pb9116-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9116-1-WB-anti-rip-picoband-antibody.jpgAnti-RIP/RIPK1 Antibody Picoband&reg; Western blot analysis of RIP using anti-RIP antibody (PB9116). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant human RIP protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 38 kDa. The expected band size for RIP is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9116-2-WB-anti-rip-picoband-antibody.jpgAnti-RIP/RIPK1 Antibody Picoband&reg; Western blot analysis of RIP using anti-RIP antibody (PB9116). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates, <br> Lane 2: human 22RV1 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP antigen affinity purified polyclonal antibody (Catalog # PB9116) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP at approximately 76 kDa. The expected band size for RIP is at 76 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-scp3-antibody-rp1035-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9117-1-RP1035-WB-anti-scp3-antibody.jpgAnti-SCP3/SYCP3 Antibody Picoband&reg;Anti-SCP3 Picoband antibody&#44; RP1035-1.jpg<br>All lanes: Anti SCP3 (RP1035) at 0.5ug/ml<br>WB: Recombinant Human SCP3 Protein 0.5ng<br>Predicted bind size: 41KD <br>Observed bind size: 41KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9117-2-RP1035-WB-anti-scp3-antibody.jpgAnti-SCP3/SYCP3 Antibody Picoband&reg;Anti-SCP3 Picoband antibody&#44; RP1035-2.jpg<br>All lanes: Anti SCP3 (RP1035) at 0.5ug/ml<br>WB: HT1080 Whole Cell Lysate at 40ug<br>Predicted bind size: 28KD<br>Observed bind size: 28KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ap2-alpha-picoband-trade-antibody-pb9118-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9118-1-WB-anti-ap2-alpha-picoband-antibody.jpgAnti-AP2 alpha/TFAP2A Antibody Picoband&reg; Western blot analysis of AP2 alpha using anti-AP2 alpha antibody (PB9118). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant human AP2A protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2 alpha antigen affinity purified polyclonal antibody (Catalog # PB9118) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2 alpha at approximately 38 kDa. The expected band size for AP2 alpha is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9118-2-WB-anti-ap2-alpha-picoband-antibody.jpgAnti-AP2 alpha/TFAP2A Antibody Picoband&reg; Western blot analysis of AP2 alpha using anti-AP2 alpha antibody (PB9118). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2 alpha antigen affinity purified polyclonal antibody (Catalog # PB9118) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2 alpha at approximately 48 kDa. The expected band size for AP2 alpha is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tie2-antibody-rp1036-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9119-1-RP1036-WB-anti-tie2-antibody.jpgAnti-TIE2/TEK Antibody Picoband&reg;Anti-TIE2 Picoband antibody&#44; RP1036-1.jpg<br>All lanes: Anti TIE2 (RP1036) at 0.5ug/ml<br>WB: Recombinant Human TIE2 Protein 0.5ng<br>Predicted bind size: 47KD<br>Observed bind size: 47KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9119-2-RP1036-WB-anti-tie2-antibody.jpgAnti-TIE2/TEK Antibody Picoband&reg;Anti-TIE2 Picoband antibody&#44; RP1036-2.jpg<br>All lanes: Anti TIE2 (RP1036) at 0.5ug/ml<br>WB: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 125KD<br>Observed bind size: 125KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hamartin-picoband-trade-antibody-pb9120-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9120-1-WB-anti-hamartin-picoband-antibody.jpgAnti-Hamartin/TSC1 Antibody Picoband&reg; Western blot analysis of Hamartin using anti-Hamartin antibody (PB9120). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human Hamartin protein 0.5 ng<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin antigen affinity purified polyclonal antibody (Catalog # PB9120) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hamartin at approximately 38 kDa. The expected band size for Hamartin is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9120-2-WB-anti-hamartin-picoband-antibody.jpgAnti-Hamartin/TSC1 Antibody Picoband&reg; Western blot analysis of Hamartin using anti-Hamartin antibody (PB9120). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin antigen affinity purified polyclonal antibody (Catalog # PB9120) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hamartin at approximately 130 kDa. The expected band size for Hamartin is at 130 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tuberin-picoband-trade-antibody-pb9121-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9121-1-WB-anti-tuberin-picoband-antibody.jpgAnti-Tuberin/TSC2 Antibody Picoband&reg; Western blot analysis of Tuberin using anti-Tuberin antibody (PB9121). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human Tuberin protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tuberin antigen affinity purified polyclonal antibody (Catalog # PB9121) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tuberin at approximately 41 kDa. The expected band size for Tuberin is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9121-2-WB-anti-tuberin-picoband-antibody.jpgAnti-Tuberin/TSC2 Antibody Picoband&reg; Western blot analysis of Tuberin using anti-Tuberin antibody (PB9121). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U20S whole cell lysates, <br> Lane 2: human PANC whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human COLO320 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tuberin antigen affinity purified polyclonal antibody (Catalog # PB9121) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tuberin at approximately 201 kDa. The expected band size for Tuberin is at 201 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9121-4-IHC-anti-tuberin-picoband-antibody.jpgAnti-Tuberin/TSC2 Antibody Picoband&reg; IHC analysis of Tuberin using anti-Tuberin antibody (PB9121). <br> Tuberin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Tuberin Antibody (PB9121) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9121-3-IHC-anti-tuberin-picoband-antibody.jpgAnti-Tuberin/TSC2 Antibody Picoband&reg; IHC analysis of Tuberin using anti-Tuberin antibody (PB9121). <br> Tuberin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Tuberin Antibody (PB9121) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9121-5-IHC-anti-tuberin-picoband-antibody.jpgAnti-Tuberin/TSC2 Antibody Picoband&reg; IHC analysis of Tuberin using anti-Tuberin antibody (PB9121). <br> Tuberin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Tuberin Antibody (PB9121) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ubiquitin-picoband-trade-antibody-pb9122-boster.html2026-03-24T05:04:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9122-1_2.jpgAnti-Ubiquitin/UBB Antibody Picoband&reg; Western blot analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates&#44;<br> Lane 2: human K562 whole cell lysates&#44; <br> Lane 3: human THP-1 whole cell lysates&#44; <br> Lane 4: rat PC-12 whole cell lysates&#44; <br> Lane 5: rat brain tissue lysates&#44;<br> Lane 6: mouse brain tissue lysates&#44;<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubiquitin antigen affinity purified polyclonal antibody (Catalog # PB9122) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubiquitin at approximately 9KD. The expected band size for Ubiquitin is at 9KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9122-2-IHC-anti-ubiquitin-picoband-antibody.jpgAnti-Ubiquitin/UBB Antibody Picoband&reg; IHC analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122).<br> Ubiquitin was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ubiquitin Antibody (PB9122) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9122-3-IHC-anti-ubiquitin-picoband-antibody.jpgAnti-Ubiquitin/UBB Antibody Picoband&reg; IHC analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122).<br> Ubiquitin was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ubiquitin Antibody (PB9122) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9122-4-IHC-anti-ubiquitin-picoband-antibody.jpgAnti-Ubiquitin/UBB Antibody Picoband&reg; IHC analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122).<br> Ubiquitin was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ubiquitin Antibody (PB9122) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9122-ubiquitin-primary-antibodies-if-testing-5.jpgAnti-Ubiquitin/UBB Antibody Picoband&reg; IF analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). <br> Ubiquitin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Ubiquitin Antibody (PB9122) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-tnr-antibody-pa1695-boster.html2026-03-24T05:04:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-tnr-primary-antibodies-ihc-testing-5.jpgAnti-Tenascin-R TNR Antibody Picoband&reg;IHC analysis of Tenascin-R/TNR using anti-Tenascin-R/TNR antibody (PA1695). <br>Tenascin-R/TNR was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tenascin-R/TNR Antibody (PA1695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-tnr-primary-antibodies-wb-testing-1.jpgAnti-Tenascin-R TNR Antibody Picoband&reg; Western blot analysis of TNR using anti-TNR antibody (PA1695). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNR antigen affinity purified polyclonal antibody (Catalog # PA1695) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNR at approximately 180-250 kDa. The expected band size for TNR is at 150 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-tnr-primary-antibodies-ihc-testing-2.jpgAnti-Tenascin-R TNR Antibody Picoband&reg; IHC analysis of TNR using anti-TNR antibody (PA1695). <br> TNR was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNR Antibody (PA1695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-tnr-primary-antibodies-ihc-testing-3.jpgAnti-Tenascin-R TNR Antibody Picoband&reg; IHC analysis of TNR using anti-TNR antibody (PA1695). <br> TNR was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNR Antibody (PA1695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-tnr-primary-antibodies-ihc-testing-4.jpgAnti-Tenascin-R TNR Antibody Picoband&reg; IHC analysis of TNR using anti-TNR antibody (PA1695). <br> TNR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TNR Antibody (PA1695) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/a/pa1695-tnr-primary-antibodies-fcm-testing-5.jpgAnti-Tenascin-R TNR Antibody Picoband&reg; Flow Cytometry analysis of SH-SY5Y cells using anti-TNR antibody (PA1695). <br> Overlay histogram showing SH-SY5Y cells stained with PA1695 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNR Antibody (PA1695, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/picokine-elisa-kits/human-igf-1-picokine-elisa-kit-ek0376-boster.html2026-03-24T05:04:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0376_2.pngHuman IGF-1 ELISA Kit PicoKine®Human IGF-1 PicoKine ELISA Kit Standard Curve https://www.bosterbio.com/picokine-elisa-kits/mouse-leptin-picokine-trade-elisa-kit-ek0438-boster.html2026-03-24T05:04:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0438.pngMouse Leptin ELISA Kit PicoKine®Mouse Leptin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-sdf-1-picokine-trade-elisa-kit-ek0500-boster.html2026-03-24T05:04:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0500.pngMouse SDF-1/CXCL12 ELISA Kit PicoKine®Mouse SDF-1/CXCL12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pdgf-bb-picokine-trade-elisa-kit-ek0956-boster.html2026-03-24T05:04:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0956.jpgHuman PDGF-BB ELISA Kit PicoKine®Human PDGF-BB PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-12-lipoxygenase-alox12-ubb-antibody-pa1485-1-boster.html2026-03-24T05:04:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pa1485-1-1_1-WB-anti-12-lipoxygenase-antibody.jpgAnti-12 Lipoxygenase/ALOX12 Antibody Picoband&reg;Anti-12 Lipoxygenase antibody&#44; PA1485-1&#44; Western blotting<br>All lanes: Anti ANOX12 (PA1485-1) at 0.5ug/ml<br> Lane 1: A549 Whole Cell Lysate at 40ug<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug<br> Lane 3: COLO320 Whole Cell Lysate at 40ug<br> Lane 4: JURKAT Whole Cell Lysate at 40ug<br> Lane 5: HELA Whole Cell Lysate at 40ug<br> Predicted bind size: 75KD<br> Observed bind size: 75KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ace-picoband-trade-antibody-pb9124-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-1_1.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; Western blot analysis of ACE using anti-ACE antibody (PB9124). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates&#44; <br> Lane 2: mouse lung tissue lysates&#44; <br> Lane 3: human Raji whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE antigen affinity purified polyclonal antibody (Catalog # PB9124) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE at approximately 180KD. The expected band size for ACE is at 150KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-ace-primary-antibodies-ihc-testing-2.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; IHC analysis of ACE using anti-ACE antibody (PB9124).<br> ACE was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-ace-primary-antibodies-ihc-testing-3.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; IHC analysis of ACE using anti-ACE antibody (PB9124).<br> ACE was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-ace-primary-antibodies-ihc-testing-4.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; IHC analysis of ACE using anti-ACE antibody (PB9124).<br> ACE was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-ace-primary-antibodies-ihc-testing-5.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; IHC analysis of ACE using anti-ACE antibody (PB9124).<br> ACE was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-6.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; IHC analysis of ACE using anti-ACE antibody (PB9124). <br> ACE was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-7.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; IHC analysis of ACE using anti-ACE antibody (PB9124). <br> ACE was detected in frozen section of mouse lung tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACE Antibody (PB9124) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9124-8.jpgAnti-Angiotensin Converting Enzyme 1/ACE Antibody Picoband&reg; IF analysis of ACE using anti-ACE antibody (PB9124).<br> ACE was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ACE Antibody (PB9124) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angiopoietin-1-picoband-trade-antibody-pb9125-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9125-1-WB-anti-angiopoietin-1-antibody.jpgAnti-Angiopoietin 1/ANGPT1 Antibody Picoband&reg; Western blot analysis of Angiopoietin 1 using anti-Angiopoietin 1 antibody (PB9125). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant human Angiopoietin1 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin 1 antigen affinity purified polyclonal antibody (Catalog # PB9125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin 1 at approximately 39 kDa. The expected band size for Angiopoietin 1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9125-2-WB-anti-angiopoietin-1-antibody.jpgAnti-Angiopoietin 1/ANGPT1 Antibody Picoband&reg; Western blot analysis of Angiopoietin 1 using anti-Angiopoietin 1 antibody (PB9125). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human COLO320 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human HepG2 whole cell lysates, <br> Lane 6: human 293T whole cell lysates, <br> Lane 7: human SW620 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin 1 antigen affinity purified polyclonal antibody (Catalog # PB9125) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin 1 at approximately 65 kDa. The expected band size for Angiopoietin 1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9125-fnins-18-1398913-g012.jpgAnti-Angiopoietin 1/ANGPT1 Antibody Picoband&reg;(A) In the schematic brain diagram, the orange area represents the infarct area. Red boxes indicate the regions where immunofluorescence images were taken. The left side shows the ingrowth of lymphatic vessels on day 28, past stroke. Scale bar: 50 μm. (B) The distribution of meningeal lymphatic vessels on day 3 post-stroke in young FTG mice. The white arrow indicates the growth of lymphatic vessels. R: right side, L: left side. Scale bar: 1,000 μm. (C) Representative Western blot images of VEGF/GAPDH and Ang1/GAPDH in the cortex on day 28 post-stroke. (D) Quantification of VEGF/GAPDH and Ang1/GAPDH relative expression on day 28 post-stroke ( n = 5 per group). (E) Representative Western blot images of PROX1/GAPDH, Lyve-1/GAPDH, and VEGFC/β-actin in the cortex on day 28 post-stroke ( n = 5 per group). (F) Quantification of PROX1/GAPDH, Lyve-1/GAPDH, and VEGFC/β-actin relative expression on day 28 post-stroke.* P < 0.05, ** indicates a p- value of < 0.01 in the aged-FTG vs. young-FTG group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2024.1398913/full'>39371609</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9125-fped-08-00190-g006.jpgAnti-Angiopoietin 1/ANGPT1 Antibody Picoband&reg;Hyperoxia down-regulated pulmonary Twist1, Tie1, Tie2, and Ang1 mRNAs, while up-regulated Ang2 mRNA. Lungs from all different groups were excised, total RNA was isolated, and Twist1 (A) , Tie1 (B) , Tie2 (C) , Ang1 (D) , and Ang2 (E) mRNA levels were determined by real time-PCR following cDNA synthesis, as described in the Materials and Methods section ( n = 6/group). * P < 0.05, ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2020.00190/full'>32391293</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9125-fped-08-00190-g007.jpgAnti-Angiopoietin 1/ANGPT1 Antibody Picoband&reg;Hyperoxia reduced pulmonary Twist1, Tie1, Tie2, and Ang1 protein levels, while up-regulated Ang2 protein level. The lung homogenates prepared from different groups of newborn rats were subjected to western blots analysis. Western blots showed the expression of Twist1 (A,D) , Ang1/2 (A,C) , Tie1 (B,F) , pTie2/Tie2 (B,E) in the lungs. The densitometric intensities of these proteins normalized to Gapdh were quantified and shown separately ( n = 8–14/group). * P < 0.05, ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2020.00190/full'>32391293</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-angiopoietin-2-antibody-rp1037-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1037-angpt2-primary-antibodies-ihc-testing-1.jpgAnti-Angiopoietin 2/ANGPT2 AntibodyIHC analysis of ANGPT2 using anti-ANGPT2 antibody (RP1037). <br> ANGPT2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPT2 Antibody (RP1037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1037-angpt2-primary-antibodies-ihc-testing-2.jpgAnti-Angiopoietin 2/ANGPT2 AntibodyIHC analysis of ANGPT2 using anti-ANGPT2 antibody (RP1037). <br> ANGPT2 was detected in a paraffin-embedded section of human adrenal adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPT2 Antibody (RP1037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1037-angpt2-primary-antibodies-ihc-testing-3.jpgAnti-Angiopoietin 2/ANGPT2 AntibodyIHC analysis of ANGPT2 using anti-ANGPT2 antibody (RP1037). <br> ANGPT2 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPT2 Antibody (RP1037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1037-angpt2-primary-antibodies-ihc-testing-4.jpgAnti-Angiopoietin 2/ANGPT2 AntibodyIHC analysis of ANGPT2 using anti-ANGPT2 antibody (RP1037). <br> ANGPT2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPT2 Antibody (RP1037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1037-angpt2-primary-antibodies-ihc-testing-5.jpgAnti-Angiopoietin 2/ANGPT2 AntibodyIHC analysis of ANGPT2 using anti-ANGPT2 antibody (RP1037). <br> ANGPT2 was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPT2 Antibody (RP1037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1037-angpt2-primary-antibodies-ihc-testing-6.jpgAnti-Angiopoietin 2/ANGPT2 AntibodyIHC analysis of ANGPT2 using anti-ANGPT2 antibody (RP1037). <br> ANGPT2 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ANGPT2 Antibody (RP1037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a1-picoband-trade-antibody-pb9127-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9127-anax1-primary-antibodies-wb-testing-1.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg; Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (PB9127). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: monkey COS-7 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human PC-3 whole cell lysates, <br> Lane 6: human U20S whole cell lysates, <br> Lane 7: rat spleen tissue lysates, <br> Lane 8: rat lung tissue lysates, <br> Lane 9: rat kidney tissue lysates, <br> Lane 10: rat C6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A1/ANXA1 antigen affinity purified polyclonal antibody (Catalog # PB9127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A1/ANXA1 at approximately 39KD. The expected band size for Annexin A1/ANXA1 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9127-annexin-a1-primary-antibodies-wb-testing-4.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg; Western blot analysis of Annexin A1 using anti-Annexin A1 antibody (PB9127). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U-87MG whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: monkey COS-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A1 antigen affinity purified polyclonal antibody (PB9127) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for Annexin A1 at approximately 35 kDa. The expected band size for Annexin A1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9127-2-ihc-anti-annexin-a1-picoband-antibody.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg; IHC analysis of Annexin A1 using anti-Annexin A1 antibody (PB9127). <br> Annexin A1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin A1 Antibody (PB9127) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9127-3-ihc-anti-annexin-a1-picoband-antibody.jpgAnti-Annexin A1/ANXA1 Antibody Picoband&reg; IHC analysis of Annexin A1 using anti-Annexin A1 antibody (PB9127). <br> Annexin A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Annexin A1 Antibody (PB9127) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ape1-picoband-trade-antibody-pb9128-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9128-apex1-primary-antibodies-wb-testing-1.jpgAnti-APE1/APEX1 Antibody Picoband&reg; Western blot analysis of APEX1 using anti-APEX1 antibody (PB9128). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: human A549 whole cell lysates,<br> Lane 6: human MCF-7 whole cell lysates,<br> Lane 7: human RT4 whole cell lysates,<br> Lane 8: human U87 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody (Catalog # PB9128) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9128-apex1-primary-antibodies-wb-testing-2_1.jpgAnti-APE1/APEX1 Antibody Picoband&reg; Western blot analysis of APEX1 using anti-APEX1 antibody (PB9128). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human RT4 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody (Catalog # PB9128) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9128-3-IHC-anti-ape1-picoband-antibody.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PB9128).<br> APEX1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APEX1 Antibody (PB9128) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9128-4-IHC-anti-ape1-picoband-antibody.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PB9128).<br> APEX1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APEX1 Antibody (PB9128) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9128-5-IHC-anti-ape1-picoband-antibody.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IHC analysis of APEX1 using anti-APEX1 antibody (PB9128).<br> APEX1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APEX1 Antibody (PB9128) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9128-6.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IF analysis of APEX1 using anti-APEX1 antibody (PB9128).<br> APEX1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-APEX1 Antibody (PB9128) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9128-7.pngAnti-APE1/APEX1 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-APEX1 antibody (PB9128). <br> Overlay histogram showing U937 cells stained with PB9128 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APEX1 Antibody (PB9128&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9128-apex1-primary-antibodies-if-testing-8.jpgAnti-APE1/APEX1 Antibody Picoband&reg; IF analysis of APEX1 using anti-APEX1 antibody (PB9128).<br> APEX1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-APEX1 Antibody (PB9128) overnight at 4°C.DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcl1-picoband-trade-antibody-pb9132-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9132-1-WB-anti-mcl1-picoband-antibody.jpgAnti-MCL1 Antibody Picoband&reg; Western blot analysis of MCL1 using anti-MCL1 antibody (PB9132). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human MCL1 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCL1 antigen affinity purified polyclonal antibody (Catalog # PB9132) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCL1 at approximately 40 kDa. The expected band size for MCL1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9132-2-WB-anti-mcl1-picoband-antibody.jpgAnti-MCL1 Antibody Picoband&reg; Western blot analysis of MCL1 using anti-MCL1 antibody (PB9132). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human COLO320 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCL1 antigen affinity purified polyclonal antibody (Catalog # PB9132) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCL1 at approximately 37 kDa. The expected band size for MCL1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9132-12885_2023_10974_fig2_html.pngAnti-MCL1 Antibody Picoband&reg;CYD0281 promotes HUVECs cell apoptosis by the exposure of the Bcl-2 BH3 domain. A Cell viability of HUVECs at 48 h. The exposure of the BH3 domain of Bcl-2 detected by immunofluorescence (IF) staining using anti-Bcl-2/BH3 domain antibody ( B ), and flow cytometry analysis of cell apoptosis ( C ) in HUVECs treated with BDA-366 and CYD0281 at the IC 50 concentration. HUVECs were treated with CYD0281 at the IC 50 concentration for 48 h, mitochondrial dysfunction was analyzed by Mito-Tracker Red CMXRos staining ( D ), Cyt c release ( E ) and PARP cleavage ( F ) were analyzed by western blotting assay, and Bcl-2 associated Bax or Bim was analyzed by co-IP using Bcl-2 antibody ( G ). H The expression of Bcl-2 in HUVECs was inhibited using siRNAs (1, 2, and 3) and analyzed by western blotting assay. I Flow cytometry analysis of cell apoptosis in Bcl-2-silenced HUVECs that were treated with CYD0281 ( n = 3). Significant effect compared to the DMSO group: * P < 0.05 and *** P < 0.001. ns: no significant differences. Scale bars: 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-023-10974-4'>37237269</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9132-12885_2023_10974_fig3_html.pngAnti-MCL1 Antibody Picoband&reg;CYD0281 inhibits angiogenic differentiation of HUVECs in vitro. Representative microscopic images and quantification of cell migration ( A ), and tube formation in Matrigel ( B ). C Representative images of endothelial cells sprout and quantification from rat aortic ring assay at 8 days of treatment. Significant effect compared to the DMSO group: ** P < 0.01 and *** P < 0.001. Scale bars: 100 μm ( A and B ) and 500 μm ( C ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12885-023-10974-4'>37237269</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9132-3-IHC-anti-mcl1-picoband-antibody.jpgAnti-MCL1 Antibody Picoband&reg; IHC analysis of MCL1 using anti-MCL1 antibody (PB9132). <br> MCL1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCL1 Antibody (PB9132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9132-4-IHC-anti-mcl1-picoband-antibody.jpgAnti-MCL1 Antibody Picoband&reg; IHC analysis of MCL1 using anti-MCL1 antibody (PB9132). <br> MCL1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCL1 Antibody (PB9132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9132-5-IHC-anti-mcl1-picoband-antibody.jpgAnti-MCL1 Antibody Picoband&reg; IHC analysis of MCL1 using anti-MCL1 antibody (PB9132). <br> MCL1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCL1 Antibody (PB9132) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmi1-picoband-trade-antibody-pb9133-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9133-1_1.jpgAnti-Bmi1 Antibody Picoband&reg; Western blot analysis of BMI1 using anti-BMI1 antibody (PB9133). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U2O whole cell lysates<br> Lane 2: human PC-3 whole cell lysates<br> Lane 3: human HEK293 whole cell lysates<br> Lane 4: human Caco-2 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 antigen affinity purified polyclonal antibody (Catalog # PB9133) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMI1 at approximately 43-45KD. The expected band size for BMI1 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9133-2_1.jpgAnti-Bmi1 Antibody Picoband&reg; Western blot analysis of BMI1 using anti-BMI1 antibody (PB9133).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates<br> Lane 2: mouse brain tissue lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMI1 antigen affinity purified polyclonal antibody (Catalog # PB9133) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMI1 at approximately 43-45KD. The expected band size for BMI1 is at 39KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-syncam-picoband-trade-antibody-pb9136-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9136-syncam-primary-antibodies-wb-testing-1.jpgAnti-SynCAM/CADM1 Antibody Picoband&reg; Western blot analysis of SynCAM using anti-SynCAM antibody (PB9136). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: rat liver tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SynCAM antigen affinity purified polyclonal antibody (Catalog # PB9136) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SynCAM at approximately 49 kDa. The expected band size for SynCAM is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd18-picoband-trade-antibody-pb9141-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9141-itgb2-primary-antibodies-wb-testing-1.jpgAnti-CD18/ITGB2 Antibody Picoband&reg; Western blot analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD18/ITGB2 antigen affinity purified polyclonal antibody (Catalog # PB9141) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD18/ITGB2 at approximately 90-100 kDa. The expected band size for CD18/ITGB2 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9141-itgb2-primary-antibodies-ihc-testing-2.jpgAnti-CD18/ITGB2 Antibody Picoband&reg; IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141). <br> CD18/ITGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD18/ITGB2 Antibody (PB9141) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9141-itgb2-primary-antibodies-ihc-testing-3.jpgAnti-CD18/ITGB2 Antibody Picoband&reg; IHC analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141). <br> CD18/ITGB2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD18/ITGB2 Antibody (PB9141) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9141-itgb2-primary-antibodies-if-testing-4.jpgAnti-CD18/ITGB2 Antibody Picoband&reg; IF analysis of CD18/ITGB2 using anti-CD18/ITGB2 antibody (PB9141). <br> CD18/ITGB2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD18/ITGB2 Antibody (PB9141) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mrp4-picoband-trade-antibody-pb9144-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9144-1-WB-anti-mrp4-picoband-antibody.jpgAnti-MRP4/ABCC4 Antibody Picoband&reg; Western blot analysis of MRP4 using anti-MRP4 antibody (PB9144). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human MRP4 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MRP4 antigen affinity purified polyclonal antibody (Catalog # PB9144) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MRP4 at approximately 47 kDa. The expected band size for MRP4 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9144-2-IHC-anti-mrp4-picoband-antibody.jpgAnti-MRP4/ABCC4 Antibody Picoband&reg; IHC analysis of MRP4 using anti-MRP4 antibody (PB9144). <br> MRP4 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MRP4 Antibody (PB9144) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9144-3-IHC-anti-mrp4-picoband-antibody.jpgAnti-MRP4/ABCC4 Antibody Picoband&reg; IHC analysis of MRP4 using anti-MRP4 antibody (PB9144). <br> MRP4 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MRP4 Antibody (PB9144) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9144-4-IHC-anti-mrp4-picoband-antibody.jpgAnti-MRP4/ABCC4 Antibody Picoband&reg; IHC analysis of MRP4 using anti-MRP4 antibody (PB9144). <br> MRP4 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MRP4 Antibody (PB9144) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-muc1-picoband-trade-antibody-pb9145-boster.html2026-03-16T09:13:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9145-1-IHC-anti-muc1-picoband-antibody.jpgAnti-MUC1 Antibody IHC analysis of MUC1 using anti-MUC1 antibody (PB9145). <br> MUC1 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MUC1 Antibody (PB9145) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-myd88-picoband-trade-antibody-pb9148-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-myd88-primary-antibodies-wb-testing-1.jpgAnti-MyD88 Antibody Picoband&reg;Western blot analysis of MyD88 using anti-MyD88 antibody (PB9148). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse HEPA1-6 whole cell lysates,<br> Lane 7: mouse Raw264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MyD88 antigen affinity purified polyclonal antibody (PB9148) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MyD88 at approximately 36 kDa. The expected band size for MyD88 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-10.jpgAnti-MyD88 Antibody Picoband&reg; IF analysis of MYD88 using anti-MYD88 antibody (PB9148)<br> MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9148-3-IHC-anti-myd88-picoband-antibody.jpgAnti-MyD88 Antibody Picoband&reg; IHC analysis of MYD88 using anti-MYD88 antibody (PB9148).<br> MYD88 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9148-4-IHC-anti-myd88-picoband-antibody.jpgAnti-MyD88 Antibody Picoband&reg; IHC analysis of MYD88 using anti-MYD88 antibody (PB9148).<br> MYD88 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9148-5-IHC-anti-myd88-picoband-antibody.jpgAnti-MyD88 Antibody Picoband&reg; IHC analysis of MYD88 using anti-MYD88 antibody (PB9148).<br> MYD88 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-6.jpgAnti-MyD88 Antibody Picoband&reg; IF analysis of MYD88 using anti-MYD88 antibody (PB9148)<br> MYD88 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-7.jpgAnti-MyD88 Antibody Picoband&reg; IF analysis of MYD88 using anti-MYD88 antibody (PB9148)<br> MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-8.jpgAnti-MyD88 Antibody Picoband&reg; IF analysis of MYD88 using anti-MYD88 antibody (PB9148)<br> MYD88 was detected in paraffin-embedded section of human colon cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-9.jpgAnti-MyD88 Antibody Picoband&reg; IF analysis of MYD88 using anti-MYD88 antibody (PB9148)<br> MYD88 was detected in paraffin-embedded section of human mammary cancar tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-11.jpgAnti-MyD88 Antibody Picoband&reg; IF analysis of MYD88 using anti-MYD88 antibody (PB9148)<br> MYD88 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MYD88 Antibody (PB9148) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-12.pngAnti-MyD88 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-MYD88 antibody (PB9148). <br> Overlay histogram showing A549 cells stained with PB9148 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MYD88 Antibody (PB9148&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9148-fphar-15-1430599-g005.jpgAnti-MyD88 Antibody Picoband&reg;MT regulated brain barrier function and TLR4/MyD88/NF-κB signaling pathway in sleep-deprived rats. (A) Western blot bands showing the protein expression levels of Iba1 and Aβ42 in the HP, respectively. (B, C) Relative protein expression level of Iba1 and Aβ42 in the HP, respectively. (D) Western blot brands showing the protein expression levels of ZO-1 and occludin in the HP, respectively. (E) Western blot brands showing the protein expression levels of TLR4, MyD88, IKB-α, p-IKB-α, NF-κB p65, and p-NF-κB p65 in the HP, respectively. (F, G) Relative protein expression level of ZO-1 and occludin in the HP, respectively. (H–K) Relative protein expression level of TLR4, MyD88, p-IKB-α/IKB-α, and p-NF-κB p65/NF-κB p65, respectively. (L–N) Relative mRNA expression of ZO-1, occluding, and TLR4 in the HP, respectively. The data are expressed as the means ± SEM. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Control group; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. Model group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1430599/full'>39101143</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfkb-p105-p50-picoband-trade-antibody-pb9149-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9149-41467_2018_7654_fig5_html.pngAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg;FLIP activates the canonical NF-κB pathway in myeloid cells. a Suppressive activity of THP1 cells transfected with c-FLIP RNA was measured by enumeration of an absolute number of human CD3 + T cells collected after in vitro co-culture. b Immunofluorescence confocal microscopy of p65 (red), p50 and p52 (green) nuclear translocation in transfected THP1 cells. c Nuclear c-FLIP protein expression by western blot in THP1 cells transfected with either GFP or c-FLIP RNA at different time points. d Immunofluorescence confocal microscopy of p50 (red) and c-FLIP (green) nuclear translocation in transfected THP1 cells. e CD11b + Ly6C + cells (M-MDSCs) were isolated from the spleen of MCA203 tumor-bearing, wild-type mice by flow sorter and transfected for 18 h with scramble, IKKα, IKKβ, or the combination IKKα plus IKKβ siRNAs. After transfection, cells were washed three times. M-MDSCs were co-incubated with peptide-stimulated CellTrace-labeled OT-I cells. Suppressive activity was measured by enumerating absolute numbers of CD8 + T cells collected after in vitro co-culture. f PD-L1 expression in transfected M-MDSCs. Data are presented either as mean ± s.e.m of three independent experiments ( a ) or as mean ± s.e.m of four independent experimental transfections ( e , f ) where each plot refers to CD11b + Ly6C + cells isolated from the pooled spleens of three tumor-bearing mice. Original images × 800 for all panels ( b , d ). * P < 0.05, ** P < 0.01; *** P < 0.001; n.s., not significant, by Mann–Whitney test ( a , b , e , f ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-018-07654-4'>30518925</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9149-nfkb1-primary-antibodies-fcm-testing-3.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-NFkB p105/p50/NFKB1 antibody (PB9149). <br>Overlay histogram showing Hela cells stained with PB9149 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFkB p105/p50/NFKB1 Antibody (PB9149, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9149-nfkb1-primary-antibodies-wb-testing-1.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg; Western blot analysis of NFkB p105/p50/NFKB1 using anti-NFkB p105/p50/NFKB1 antibody (PB9149). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates, <br> Lane 2: human SH-SY5Y whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFkB p105/p50/NFKB1 antigen affinity purified polyclonal antibody (Catalog # PB9149) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFkB p105/p50/NFKB1 at approximately 50 kDa, 120 kDa. The expected band size for NFkB p105/p50/NFKB1 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9149-nfkb1-primary-antibodies-if-testing-2.jpgAnti-NFkB/NFKB1 p105/p50 Antibody Picoband&reg; IF analysis of NFkB p105/p50/NFKB1 using anti-NFkB p105/p50/NFKB1 antibody (PB9149). <br> NFkB p105/p50/NFKB1 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NFkB p105/p50/NFKB1 Antibody (PB9149) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfkb-p100-p52-picoband-trade-antibody-pb9150-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9150-nfkb2-primary-antibodies-wb-testing-1.jpgAnti-NFkB/NFKB2 p100/p52 Antibody Picoband&reg; Western blot analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human U87 whole cell lysates,<br> Lane 6: mouse lung tissue lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFkB/NFKB2 p100/p52 antigen affinity purified polyclonal antibody (Catalog # PB9150) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFkB/NFKB2 p100/p52 at approximately 52 kDa (active form), 120kDa (precursor). The expected band size for NFkB/NFKB2 p100/p52 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9150-2-ihc-anti-nfkb-p100-p52-picoband-antibody.jpgAnti-NFkB/NFKB2 p100/p52 Antibody Picoband&reg; IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150). <br> NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9150-3-IHC-anti-nfkb-p100-p52-picoband-antibody.jpgAnti-NFkB/NFKB2 p100/p52 Antibody Picoband&reg; IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150). <br> NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9150-4-IHC-anti-nfkb-p100-p52-picoband-antibody.jpgAnti-NFkB/NFKB2 p100/p52 Antibody Picoband&reg; IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150). <br> NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9150-5-IHC-anti-nfkb-p100-p52-picoband-antibody.jpgAnti-NFkB/NFKB2 p100/p52 Antibody Picoband&reg; IHC analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150). <br> NFkB/NFKB2 p100/p52 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NFkB/NFKB2 p100/p52 Antibody (PB9150) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p63-picoband-trade-antibody-pb9152-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9152-p63-primary-antibodies-wb-testing-1.jpgAnti-p63/TP63 Antibody Picoband&reg; Western blot analysis of p63 using anti-p63 antibody (PB9152). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p63 antigen affinity purified polyclonal antibody (Catalog # PB9152) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p63 at approximately 75 kDa. The expected band size for p63 is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9152-2-IHC-anti-p63-picoband-antibody.jpgAnti-p63/TP63 Antibody Picoband&reg; IHC analysis of p63 using anti-p63 antibody (PB9152). <br> p63 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-p63 Antibody (PB9152) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9152-p63-primary-antibodies-if-testing-3.jpgAnti-p63/TP63 Antibody Picoband&reg; IF analysis of p63 using anti-p63 antibody (PB9152). <br> p63 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-p63 Antibody (PB9152) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9152-p63-primary-antibodies-if-testing-4.jpgAnti-p63/TP63 Antibody Picoband&reg;IF analysis of p63 using anti-p63 antibody (PB9152). <br> p63 was detected in a paraffin-embedded section of human prostate hyperplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-p63 Antibody (PB9152) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9152-pb9983-primary-antibodies-if-testing-1.jpgAnti-p63/TP63 Antibody Picoband&reg;IF analysis of AMACR and TP63 using anti-AMACR antibody (PB9983) and anti-TP63 antibody (PB9152). <br> AMACR and TP63 were detected in paraffin-embedded sections of human prostatic hyperplasia tissue. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0). Tissue sections were blocked with 5% BSA. The sections were incubated overnight with 5 μg/mL rabbit Anti-TP63 antibody (PB9152). HRP-labeled secondary antibody was added dropwise and incubated for 30 minutes. TYR-570plus fluorescent dye reaction solution was then added dropwise and incubated at room temperature in the dark. Next, the sections were immersed in antigen retrieval buffer again to elute the bound antibodies. The staining steps were then repeated for the second round of labeling. Anti-AMACR antibody (PB9983) was diluted 1:50 and incubated with the sections overnight at 4°C. HRP-labeled secondary antibody was added dropwise and incubated for 30 minutes. After washing, TYR-520 plus fluorescent dye reaction solution was added dropwise and incubated at room temperature in the dark. Finally, DAPI staining solution was added dropwise to stain the nuclei, and the slides were sealed with an anti-fade mounting medium. The results were observed under a fluorescence microscope. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9152-p63-primary-antibodies-fcm-testing-4.pngAnti-p63/TP63 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-p63 antibody (PB9152). <br>Overlay histogram showing A431 cells stained with PB9152 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p63 Antibody (PB9152, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pd1-antibody-rp1039-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1039-pdcd1-primary-antibodies-ihc-testing-2.jpgAnti-PD1/PDCD1 Antibody Picoband&reg;IHC analysis of PD-1/CD279/PDCD1 using anti-PD-1/CD279/PDCD1 antibody (RP1039). <br>PD-1/CD279/PDCD1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PD-1/CD279/PDCD1 Antibody (RP1039) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1039-pd1-primary-antibodies-ihc-testing-1.jpgAnti-PD1/PDCD1 Antibody Picoband&reg; IHC analysis of PD1 using anti-PD1 antibody (RP1039). <br> PD1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PD1 Antibody (RP1039) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pd-l1-picoband-trade-antibody-pb9154-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9154-pd-l1-primary-antibodies-fcm-testing-2.jpgAnti-PD-L1/CD274 Antibody Picoband&reg; Flow Cytometry analysis of JK cells using anti-PD-L1 antibody (PB9154). <br>Overlay histogram showing JK cells stained with PB9154 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PD-L1 Antibody (PB9154, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9154-pd-l1-primary-antibodies-wb-testing-1.jpgAnti-PD-L1/CD274 Antibody Picoband&reg; Western blot analysis of PD-L1 using anti-PD-L1 antibody (PB9154). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates, <br> Lane 2: human placenta tissue lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human THP-1 whole cell lysates, <br> Lane 5: human K562 whole cell lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1 antigen affinity purified polyclonal antibody (Catalog # PB9154) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1 at approximately 40-50 kDa. The expected band size for PD-L1 is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prom1-picoband-trade-antibody-pb9156-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9156-prom1-primary-antibodies-wb-testing-1.jpgAnti-PROM1 Antibody Picoband&reg; Western blot analysis of PROM1 using anti-PROM1 antibody (PB9156). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human CACO-2 whole cell lysates, <br> Lane 2: human SW620 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROM1 antigen affinity purified polyclonal antibody (Catalog # PB9156) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROM1 at approximately 120KD. The expected band size for PROM1 is at 120KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9156-13046_2018_975_fig2_html.pngAnti-PROM1 Antibody Picoband&reg;MCF-7 ALDH1A1 affects in vitro stemness. a Representative images of tumorspheres (4x magnification) showing morphology of spheroids grown on ultra-low attachment plate. Scale bar, 100 μm. b Representative images of tumorspheres (4x magnification) of MCF-7 Scr, MCF-7 ALDH1A1KD and MCF-7 ALDH1A1 + , showing morphology of spheroids grown on ultra-low attachment plate. Scale bar, 100 μm. b1, b2, b3. Representative images of tumorspheres (10x magnification) of MCF-1 Scr, MCF-7 ALDH1A1KD and MCF-7 ALDH1A1 + , showing morphology of spheroids grown on ultra-low attachment plate. Scale bar, 100 μm. c Quantification of MCF-7 tumorspheres. Tumorspheres area were calculated using ImageJ Software. Ten pictures for each well were quantified. Tumorspheres> 10.000 pixel square were considered. ** p < 0.01 vs MCF-7 Scr. ### p < 0.001 vs MCF-7 ALDH1A1KD. ( n = 3). d Western blot analysis of stemness markers CD133 and KLF4 in MCF-7 Scr, MCF-7 ALDH1A1KD, and MCF-7 ALDH1A1 + tumorspheres. e Western blot analysis of ALDH1A1, HIF-1α and VEGF in MCF-7 Scr, MCF-7 ALDH1A1KD and MCF-7 ALDH1A1 + tumorspheres <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-018-0975-0'>30541574</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9156-13046_2018_975_fig3_html.pngAnti-PROM1 Antibody Picoband&reg;MCF-7 ALDH1A1 regulates angiogenic factor output via retinoic acid signalling. a Angiogenic factor release evaluated by ELISA plate array in supernatants of MCF-7 treated with CM037 (1 μM) for 48 h. The experiment was performed 2 times in duplicate. b MCF-7 cells were exposed to CM037 at different concentrations (1 and 10 μM) for 18 h and western blot was carried out. β-Actin was used to normalize loading. c Cells were treated with CM037 (1 μM, 18 h) and VEGF levels were measured by ELISA assay in MCF-7 conditioned media. After 18 h supernatants were harvested and cells fixed, stained and counted. The number of counted cells was not significantly different. Data are reported as pg/ml. ** p < 0.01 vs untreated cells. d RT-PCR analysis of VEGF in MCF-7 Scr, MCF-7 ALDH1A1KD and MCF-7 ALDH1A1 + cultured in medium with 1% FBS for 48 h. Data are reported as ΔCt (Ct gene of interest-Ct Housekeeping gene). *** p < 0.001 vs MCF-7 Scr. ### p < 0.001 vs MCF-7 ALDH1A1KD. e Western blot analysis of VEGF and HIF-1α in MCF-7 exposed or not to CoCl 2 (100 μM, 72 h, 1% FBS). β-Actin was used as loading control. Gel shown is representative of three experiments with similar results. f Quantification of blots reported in e . * p < 0.05 vs MCF-7 Scr. ** p < 0.01 vs MCF-7 Scr. ### p < 0.001 vs MCF-7 ALDH1A1KD. g Soluble VEGF was detected by ELISA in media conditioned by MCF-7 cells. Cells were seeded in 24-well plates at density 3 × 10 4 cells/well. After 48 h the supernatants were harvested and cells fixed, stained and counted. The number of counted cells was not significantly different. Data are reported as pg/ml. ** p < 0.01 vs MCF-7 Scr. ## p < 0.01 vs MCF-7 ALDH1A1KD. h HIF-1α and VEGF expression evaluated by western blot in MCF-7 ALDH1A1KD cells exposed for 48 h (1 μM) to exogenous retinoic acid. i HIF-1α and VEGF expression in MCF-7 ALDH1A1 + treated with RAR antagonist (AGN193109) and RXR antagonist (UVI 3003) for 48 h (each at 1 μM). β-Actin was used as loading control. Gel shown is representative of three experiments with similar results. j VEGF and CD133 expression in MCF-7 transiently silenced for HIF-1α. β-Actin was used as loading control. Gel shown is representative of three experiments with similar results <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-018-0975-0'>30541574</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9156-13046_2018_975_fig6_html.pngAnti-PROM1 Antibody Picoband&reg;ALDH1A1 influences tumor angiogenesis and VEGF production in vivo. a Evaluation of VEGF, HIF-1α and ALDH1A1 RNA in tumor samples. Frozen tumors were homogenized and RNA was extracted to perform RT-PCR analysis of VEGF, HIF-1α and ALDH1A1 mRNA. Data are reported as ΔCt (Ct gene of interest-Ct Housekeeping gene). Each bar is the mean of 6 different tumors. The experiment was repeated two times. * p < 0.05 vs Scr group. ** p < 0.01 vs Scr group. # p < 0.05 vs ALDH1A1KD group. ### p < 0.001 vs ALDH1A1KD group. b Evaluation of VEGF and ALDH1A1 proteins in tumor samples. Tissues were harvested, homogenized and sonicated. Subsequently, proteins were extracted and western blot was performed. β-Actin was used as loading control. The experiment was repeated two times. c Evaluation of mRNA for CAIX (HIF-1α target gene) and stemness markers (SOX2, NANOG, OCT-4 and TWIST) in tumor samples. Each bar is the mean of 6 different tumors. The experiment was repeated two times. # p < 0.05 vs ALDH1A1KD group. ## p < 0.01 vs ALDH1A1KD group. ### p < 0.001 vs ALDH1A1KD group. d Evaluation of HIF-1α and stemness markers (CD133, KLF4 and SOX2) proteins in tumor samples. The experiment was repeated two times. e Quantification of blots reported in d . * p < 0.05 vs Scr group. # p < 0.05 vs ALDH1A1KD group. ## p < 0.01 vs ALDH1A1KD group. f Quantification of microvessel density by human CD31 staining (magnification 20x) was done counting 5 random fields for section, each slide having five sections. ** p < 0.01 vs Scr group. ## p < 0.01 vs ALDH1A1 + group. g Representative images of immunostaining for CD31 (red) and DAPI (blue) in tumor sections from Scr (left), ALDH1A1KD (center) or ALDH1A1 + (right) mice. Pictures report different vessel densities in tumors. Magnification 20x. Scale bar, 50 μm. h Representative images of double-immunostaining for CD31 (red) and NG2 (green) in tumor sections from Scr (left), ALDH1A1KD (center) or ALDH1A1 + (right) mice. DAPI staining is blue. Magnification 40x. Scale bars, 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-018-0975-0'>30541574</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9156-prom1-primary-antibodies-ihc-testing-2.jpgAnti-PROM1 Antibody Picoband&reg; IHC analysis of PROM1 using anti-PROM1 antibody (PB9156). <br> PROM1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PROM1 Antibody (PB9156) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9156-prom1-primary-antibodies-ihc-testing-3.jpgAnti-PROM1 Antibody Picoband&reg; IHC analysis of PROM1 using anti-PROM1 antibody (PB9156). <br> PROM1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PROM1 Antibody (PB9156) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9156-prom1-primary-antibodies-fcm-testing-4.pngAnti-PROM1 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-PROM1 antibody (PB9156).<br>Overlay histogram showing CACO-2 cells stained with PB9156 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PROM1 Antibody (PB9156,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-runx2-picoband-trade-antibody-pb9158-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9158-1-WB-anti-runx2-cbfa1-picoband-antibody.jpgAnti-RUNX2 Antibody Picoband&reg; Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB9158). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human RUNX2 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB9158) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 50 kDa. The expected band size for RUNX2 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9158-2-WB-anti-runx2-cbfa1-picoband-antibody.jpgAnti-RUNX2 Antibody Picoband&reg; Western blot analysis of RUNX2 using anti-RUNX2 antibody (PB9158). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PB9158) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 56 kDa. The expected band size for RUNX2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158-fphar-15-1450154-g001.jpgAnti-RUNX2 Antibody Picoband&reg;Protective effect of MD on AGEs-induced osteoblasts. (A) Effect of MD on the survival rate of osteoblasts (n = 6); (B) ALP activity in osteoblasts (n = 6); (C) Western blotting results of OCN and RUNX2 in osteoblasts (n = 3). * P < 0.05; ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1450154/full'>39525628</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158-fphar-15-1450154-g005.jpgAnti-RUNX2 Antibody Picoband&reg;Protective effect of isovitexin on AGEs-induced osteoblasts. (A) Effect of isovitexin on the survival rate of osteoblasts (n = 6); (B) ALP activity in osteoblasts (n = 6); (C) Western blotting results of OCN and RUNX2 in osteoblasts (n = 3). ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1450154/full'>39525628</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158-wjsc-15-807-g003.jpgAnti-RUNX2 Antibody Picoband&reg;The effect of aryl hydrocarbon receptor overexpression or knockdown on the osteogenic differentiation in mouse bone marrow mesenchymal stromal cells. A: Alkaline phosphatase (ALP) staining (upper) at 7 th day and Alizarin red staining staining (lower) at 14 th day of osteogenic induction; B: Relative mRNA expression of ALP, biomineralization associated [tissue-nonspecific alkaline phosphatase (ALPL)] and runt-related transcription factor 2 (RUNX2) of overexpression-negative control and overexpression-aryl hydrocarbon receptor (AhR) mouse bone marrow mesenchymal stromal cells (mBMSCs); C: Relative ALPL and RUNX2 mRNA expression of knockdown-negative control and knockdown-AhR mBMSCs at 7 th day of osteogenic induction; D: Representative images of western blot of ALPL and RUNX2 in mBMSCs of different groups at 7 th day of osteogenic induction. oe-NC: Overexpression-negative control; oe-AhR: Overexpression-aryl hydrocarbon receptor; sh-NC: Knockdown-negative control; sh-AhR: Knockdown-aryl hydrocarbon receptor; ALPL: Tissue-nonspecific alkaline phosphatase; RUNX2: Runt-related transcription factor 2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10494570/&orig_host=www.ncbi.nlm.nih.gov'>37700822</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158-ajtr0011-0340-f4.jpgAnti-RUNX2 Antibody Picoband&reg;Effect of TSA on osteogenesis differentiation of hPDLSC. A. hPDLSCs were treated with difference concentration of TSA for 48 hrs, ALP activity assay was measured. Data were presented as the mean ± SD of three independent experiments. *, P < 0.05, **, P < 0.01 (ANOVA with Tukey’s post hoc test). B. The expression of ALP in control, TSA-treated hPDLSCs was examined using western blotting. C. The mRNA level of ALP in control, TSA-treated hPDLSCs was examined using qRT-PCR. Data were presented as the mean ± SD of three independent experiments. *, P < 0.05, **, P < 0.01 (ANOVA with Tukey’s post hoc test). D. The expression of OPN and OCN in control or TSA-treated hPDLSCs was examined using western blotting. E. The mRNA level of OPN and OCN in control or TSA-treated hPDLSCs was examined using qRT-PCR. Data were presented as the mean ± SD of three independent experiments. *, P < 0.05, **, P < 0.01 (ANOVA with Tukey’s post hoc test). F. hPDLSCs were treated with TSA. The relative gene expression of Runx2 was measured by western blotting. G. hPDLSCs were treated with TSA. The relative gene expression of Runx2 was measured by qRT-PCR. Data were presented as the mean ± SD of three independent experiments. **, P < 0.01, ***, P < 0.001 (ANOVA with Tukey’s post hoc test).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6357334/&orig_host=www.ncbi.nlm.nih.gov'>30787991</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158-ajtr0011-0340-f6.jpgAnti-RUNX2 Antibody Picoband&reg;TSA induces ERK1/2 phosphorylation and the activity of ERK1/2 is required for TSA-induced osteogenesis of hPDLSCs. A. hPDLSCs were treated with 5 μM TSA with or without UO126, PD98059, SP600125 and SB203580 for 48 hrs. The relative gene expression of Runx2 was detected by qRT-PCR. The data were presented as the mean ± SD of three independent experiments. **, P < 0.01 (ANOVA with Tukey’s post hoc test). B. Cells were treated with TSA. The phosphorylated ERK1/2 (p-ERK1/2), phosphorylated p38 (p-p38), phosphorylated JNK (p-JNK), ERK1/2, p38, JNK and β-actin were detected by western blotting. C. Cells were treated with TSA with or without UO126, PD98059 for 14 days. The mineralized nodules formation was detected by Alizarin Red S staining.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6357334/&orig_host=www.ncbi.nlm.nih.gov'>30787991</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158-13287_2018_955_fig3_html.pngAnti-RUNX2 Antibody Picoband&reg;BMSCs pretreated with EMF exhibited stronger osteogenic differentiation potential. a RUNX2, ALP, OPN, and OCN mRNA levels of two groups analyzed by RT-PCR. GAPDH used as loading control for quantification ( n = 3). b Expression of OPN and RUNX2 proteins of both groups determined by western blot analysis. Relative densitometer values quantified by ImageJ software, GAPDH used as internal control ( n = 3). c Images of Alizarin Red S staining exhibited plaques of calcified extracellular matrix of both groups. Scale bar = 100 μm. d Semi-quantitative analysis of Alizarin Red S staining among both groups ( n = 6). Data shown as mean ± SD. * P < 0.05, ** P < 0.01. EMF electromagnetic field <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-018-0955-5'>30092831</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158_1-s2.0-s2214031x25001147-gr2.jpgAnti-RUNX2 Antibody Picoband&reg;Knocking down YAP can alleviate TBHP-induced endplate chondrocyte degeneration (A, B) The YAP siRNA was synthesized and the transfection efficiency was evaluated through Western blot analysis to ascertain the expression of YAP. (C, D) Endplate chondrocytes were transfected with YAP siRNA or NC siRNA following TBHP (100 μM) induction for 6 h. Western blot was conducted to examine the protein levels of COL2, MMP3 and CTGF. The band densities were quantified and normalized. (E, F) endplate chondrocytes were transfected with YAP siRNA or NC siRNA following TBHP (100 μM) induction for 6 h. Immunofluorescence staining was performed to examine the expression of COL2 (red). Scale bar = 50 μm. Quantitative analysis of red fluorescence intensity was performed. (G, H) Endplate chondrocytes were transfected with YAP siRNA or NC siRNA following TBHP (100 μM) induction for 6 h. Western blot was conducted to examine the protein levels of COL10 and RUNX2. The band densities were quantified and normalized. (I, J) Alizarin Red staining for the identification of calcium deposition in endplate chondrocytes. A semi-quantitative analysis of the mineralized nodule in endplate chondrocytes was conducted. (K, L) Alkaline phosphatase (ALP) staining of endplate chondrocytes. A semi-quantitative analysis of alkaline phosphatase (ALP) activity in endplate chondrocytes was conducted. Data are presented as mean ± SD from 3 independent experiments. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2214031X25001147'>40688343</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158_1-s2.0-s2214031x25001147-gr4.jpgAnti-RUNX2 Antibody Picoband&reg;Ferritinophagy involves the degeneration of endplate chondrocytes induced by oxidative stress (A, B) Endplate chondrocytes were isolated and treated with FAC for 24 h, then cells were transfected with YAP siRNA or NC siRNA following TBHP (100 μM) induction for 6 h. Western blot was conducted to examine the protein levels of GPX4 and NCOA4. The band densities were quantified and normalized. (C, D) Endplate chondrocytes were isolated and treated with FAC for 24 h, then cells were transfected with YAP siRNA or NC siRNA following TBHP (100 μM) induction for 6 h. Western blot was conducted to examine the protein levels of COL2, MMP3, CTGF, COL10 and RUNX2. The band densities were quantified and normalized. (E, F) Endplate chondrocytes were transfected with NCOA4 siRNA or NC siRNA following TBHP (100 μM) induction for 6 h. Western blot was conducted to examine the protein levels of NCOA4 and FTH. The band densities were quantified and normalized. (G, H) Endplate chondrocytes were treated with or without TBHP (100 μM) induction for 6 h. Immunofluorescence staining was conducted to examine the expression and localization of LC3(green) and FTH (red). Scale bar = 50 μm. Quantitative analysis of fluorescence intensity was performed. (I, J) 3-MA and Rapa were used to respectively treat endplate chondrocytes for 10 h. Western blot was conducted to examine the protein levels of NCOA4, FTH and LC3. The band densities were quantified and normalized. (K, L) Endplate chondrocytes were treated with BafA1 or TBHP or BafA1 + TBHP, Western blot was conducted to examine the protein levels of FTH and LC3. The band densities were quantified and normalized. Data are presented as mean ± SD from 3 independent experiments. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2214031X25001147'>40688343</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158_1-s2.0-s2214031x25001147-gr6.jpgAnti-RUNX2 Antibody Picoband&reg;Targeting the regulation of NCOA4-mediated ferritinophagy through the inhibition of the YAP/TEAD1 axis to ameliorate the degeneration of endplate chondrocytes (A) Endplate chondrocytes were isolated and treated with 100 μM TBHP with increasing concentrations (0,0.01,0.1,0.25,0.5,1and 2 μM) of VP for 24 h, CCK assay was conducted to evaluate the cell viability. (B, C) Endplate chondrocytes were treated with or without VP(0.25 μM) following TBHP (100 μM) induction for 6 h. Western blot was conducted to examine the protein levels of YAP, TEAD1, GPX4, NCOA4 and FTH. The band densities were quantified and normalized. (D, E) Endplate chondrocytes were treated with or without VP(0.25 μM) following TBHP (100 μM) induction for 6 h. Immunofluorescence staining was performed to examine the expression of NCOA4 (red). Scale bar = 50 μm. Quantitative analysis of red fluorescence intensity was performed. (F, G) Immunohistochemistry for TEAD1 and NCOA4 in cartilage endplate from each group. Scale bar = 100 μm. The ratio of positive cells for TEAD1 and NCOA4 was quantified under a microscope at 400 × magnification. (H) NCOA4 was overexpressed and transfection efficiency was assessed using Western blot to determine NCOA4 expression. (I-L) Endplate chondrocytes were divided into OE-NC (overexpression-NC plasmid) + TBHP group, OE-NC + TBHP + VP group and TBHP + VP + OE-NCOA4 (overexpression-Ncoa4 plasmid) group according to the treatment. Western blot was conducted to examine the protein levels of GPX4, NCOA4, FTH, COL2, MMP3, CTGF, COL10 and RUNX2. The band densities were quantified and normalized. (M) Immunohistochemistry for COL2 in cartilage endplate from each group. Scale bar = 100 μm. The ratio of positive cells for COL2 was quantified under a microscope at 400 × magnification. Data are presented as mean ± SD from 3 or 5 independent experiments. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2214031X25001147'>40688343</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9158-runx2-primary-antibodies-if-testing-3.jpgAnti-RUNX2 Antibody Picoband&reg; IF analysis of RUNX2 using anti-RUNX2 antibody (PB9158). <br> RUNX2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-RUNX2 Antibody (PB9158) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sirt2-picoband-trade-antibody-pb9160-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9160-sirt2-primary-antibodies-wb-testing-1.jpgAnti-SIRT2 Antibody Picoband&reg; Western blot analysis of SIRT2 using anti-SIRT2 antibody (PB9160). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 antigen affinity purified polyclonal antibody (Catalog # PB9160) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT2 approximately 36 kDa. The expected band size for SIRT2 is at 43, 40, 41, 30, 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9160-2-ihc-anti-sirt2-sirtuin-2-picoband-antibody.jpgAnti-SIRT2 Antibody Picoband&reg; IHC analysis of SIRT2 using anti-SIRT2 antibody (PB9160). <br> SIRT2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT2 Antibody (PB9160) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9160-3-IHC-anti-sirt2-sirtuin-2-picoband-antibody.jpgAnti-SIRT2 Antibody Picoband&reg; IHC analysis of SIRT2 using anti-SIRT2 antibody (PB9160). <br> SIRT2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT2 Antibody (PB9160) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9160-4-IHC-anti-sirt2-sirtuin-2-picoband-antibody.jpgAnti-SIRT2 Antibody Picoband&reg; IHC analysis of SIRT2 using anti-SIRT2 antibody (PB9160). <br> SIRT2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT2 Antibody (PB9160) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tdt-picoband-trade-antibody-pb9162-boster.html2026-03-24T05:04:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9162-1-WB-anti-tdt-picoband-antibody.jpgAnti-TdT/DNTT Antibody Picoband&reg; Western blot analysis of TdT using anti-TdT antibody (PB9162). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human TDT protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 39 kDa. The expected band size for TdT is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9162-2-WB-anti-tdt-picoband-antibody.jpgAnti-TdT/DNTT Antibody Picoband&reg; Western blot analysis of TdT using anti-TdT antibody (PB9162). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Jurkat whole cel lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TdT antigen affinity purified polyclonal antibody (Catalog # PB9162) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TdT at approximately 58 kDa. The expected band size for TdT is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aamp-picoband-trade-antibody-pb9123-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9123-aamp-primary-antibodies-wb-testing-1.jpgAnti-AAMP Antibody Picoband&reg; Western blot analysis of AAMP using anti-AAMP antibody (PB9123). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human CACO-2 whole cell lysates,<br> Lane 4: human 293T whole cell lysates.<br> Lane 5: rat heart tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse heart tissue lysates,<br> Lane 8: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AAMP antigen affinity purified polyclonal antibody (PB9123) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AAMP at approximately 52 kDa. The expected band size for AAMP is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9123-aamp-primary-antibodies-ihc-testing-2.jpgAnti-AAMP Antibody Picoband&reg; IHC analysis of AAMP using anti-AAMP antibody (PB9123). <br> AAMP was detected in a paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AAMP Antibody (PB9123) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9123-aamp-primary-antibodies-fcm-testing-3.jpgAnti-AAMP Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-AAMP antibody (PB9123). <br> Overlay histogram showing MCF-7 cells stained with PB9123 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AAMP Antibody (PB9123, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hif1-beta-picoband-trade-antibody-pb9129-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9129-1-WB-anti-hif1-beta-picoband-antibody.jpgAnti-HIF1 beta/ARNT Antibody Picoband&reg; Western blot analysis of HIF1 using anti-HIF1 antibody (PB9129). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human HIF1 beta protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1 antigen affinity purified polyclonal antibody (Catalog # PB9129) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1 at approximately 49 kDa. The expected band size for HIF1 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9129-2-WB-anti-hif1-beta-picoband-antibody.jpgAnti-HIF1 beta/ARNT Antibody Picoband&reg; Western blot analysis of HIF1 using anti-HIF1 antibody (PB9129). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human U87 whole cell lysates, <br> Lane 5: human COLO320 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1 antigen affinity purified polyclonal antibody (Catalog # PB9129) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF1 at approximately 87 kDa. The expected band size for HIF1 is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atf1-picoband-trade-antibody-pb9130-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9130-atf1-primary-antibodies-wb-testing-1.jpgAnti-ATF1 Antibody Picoband&reg; Western blot analysis of ATF1 using anti-ATF1 antibody (PB9130). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human K562 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF1 antigen affinity purified polyclonal antibody (Catalog # PB9130) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF1 at approximately 38 kDa. The expected band size for ATF1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9130-atf1-primary-antibodies-if-testing-1.jpgAnti-ATF1 Antibody Picoband&reg;IF analysis of ATF1 using anti-ATF1 antibody (PB9130). <br> ATF1 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATF1 Antibody (PB9130) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9130-atf1-primary-antibodies-fc-testing-3.jpgAnti-ATF1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-ATF1 antibody (PB9130). <br>Overlay histogram showing SiHa cells stained with PB9130 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF1 Antibody (PB9130&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9130-2-ihc-anti-atf1-picoband-antibody.jpgAnti-ATF1 Antibody Picoband&reg; IHC analysis of ATF1 using anti-ATF1 antibody (PB9130).<br> ATF1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATF1 Antibody (PB9130) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atf2-picoband-trade-antibody-pb9131-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-atf2-primary-antibodies-wb-testing-1.jpgAnti-ATF2 Antibody Picoband&reg; Western blot analysis of ATF2 using anti-ATF2 antibody (PB9131). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human SH-SY5Y whole cell lysates, <br> Lane 4: human U87 whole cell lysates, <br> Lane 5: human A549 whole cell lysates, <br> Lane 6: human MOLT4 whole cell lysates, <br> Lane 7: human HEL whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody (Catalog # PB9131) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF2 at approximately 65-70 kDa. The expected band size for ATF2 is at 65-70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-atf2-primary-antibodies-wb-testing-2.jpgAnti-ATF2 Antibody Picoband&reg; Western blot analysis of ATF2 using anti-ATF2 antibody (PB9131). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat thymus tissue lysates, <br> Lane 3: mouse brain tissue lysates, <br> Lane 4: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF2 antigen affinity purified polyclonal antibody (Catalog # PB9131) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF2 at approximately 65-70 kDa. The expected band size for ATF2 is at 65-70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-atf2-primary-antibodies-fc-testing-8_1.jpgAnti-ATF2 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-ATF2 antibody (PB9131).<br>Overlay histogram showing K562 cells stained with PB9131 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF2 Antibody (PB9131&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-atf2-primary-antibodies-fc-testing-9.jpgAnti-ATF2 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-ATF2 antibody (PB9131).<br>Overlay histogram showing HepG2 cells stained with PB9131 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATF2 Antibody (PB9131&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-3-ihc-anti-atf2-picoband-antibody.jpgAnti-ATF2 Antibody Picoband&reg; IHC analysis of ATF2 using anti-ATF2 antibody (PB9131).<br> ATF2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATF2 Antibody (PB9131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-4-ihc-anti-atf2-picoband-antibody.jpgAnti-ATF2 Antibody Picoband&reg; IHC analysis of ATF2 using anti-ATF2 antibody (PB9131).<br> ATF2 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATF2 Antibody (PB9131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-5-ihc-anti-atf2-picoband-antibody.jpgAnti-ATF2 Antibody Picoband&reg; IHC analysis of ATF2 using anti-ATF2 antibody (PB9131).<br> ATF2 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATF2 Antibody (PB9131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-6-ihc-anti-atf2-picoband-antibody.jpgAnti-ATF2 Antibody Picoband&reg; IHC analysis of ATF2 using anti-ATF2 antibody (PB9131).<br> ATF2 was detected in frozen section of rat brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATF2 Antibody (PB9131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9131-7-ihc-anti-atf2-picoband-antibody.jpgAnti-ATF2 Antibody Picoband&reg; IHC analysis of ATF2 using anti-ATF2 antibody (PB9131).<br> ATF2 was detected in frozen section of mouse brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATF2 Antibody (PB9131) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bub1-picoband-trade-antibody-pb9135-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9135-bub1-primary-antibodies-wb-testing-1.jpgAnti-Bub1 Antibody Picoband&reg; Western blot analysis of BUB1 using anti-BUB1 antibody (PB9135). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates, <br> Lane 5: human HL-60 whole cell lysates, <br> Lane 6: rat testis tissue lysates, <br> Lane 7: rat heart tissue lysates, <br> Lane 8: rat PC-12 whole cell lysates, <br> Lane 9: mouse testis tissue lysates, <br> Lane 10: mouse thymus tissue lysates, <br> Lane 11: mouse heart tissue lysates, <br> Lane 12: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BUB1 antigen affinity purified polyclonal antibody (Catalog # PB9135) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BUB1 at approximately 130 kDa. The expected band size for BUB1 is at 130 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctnna1-picoband-trade-antibody-pb9137-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9137-1-WB-anti-ctnna1-alpha-1-catenin-picoband-antibody.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband&reg; Western blot analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Recombinant Human CTNNA1 Protein 0.5ng. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 antigen affinity purified polyclonal antibody (Catalog # PB9137) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 47KD. The expected band size for CTNNA1 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9137-2-WB-anti-ctnna1-alpha-1-catenin-picoband-antibody.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband&reg; Western blot analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Liver Tissue Lysate<br> Lane 2: Rat Lung Tissue Lysate<br> Lane 3: Rat Cardiac Muscle Tissue Lysate<br> Lane 4: NIH3T3 Whole Cell Lysate<br> Lane 5: PC-12 Whole Cell Lysate<br> Lane 6: HEPG2 Whole Cell Lysate<br> Lane 7: HELA Whole Cell Lysate<br> Lane 8: MCF-7 Whole Cell Lysate<br> Lane 9: HEPA Whole Cell Lysate<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 antigen affinity purified polyclonal antibody (Catalog # PB9137) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 100KD. The expected band size for CTNNA1 is at 100KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9137-3-IHC-anti-ctnna1-alpha-1-catenin-picoband-antibody.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband&reg; IHC analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137).<br> CTNNA1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTNNA1 Antibody (PB9137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9137-4-IHC-anti-ctnna1-alpha-1-catenin-picoband-antibody.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband&reg; IHC analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137).<br> CTNNA1 was detected in paraffin-embedded section of human mammary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTNNA1 Antibody (PB9137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9137-5-IHC-anti-ctnna1-alpha-1-catenin-picoband-antibody.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband&reg; IHC analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137).<br> CTNNA1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTNNA1 Antibody (PB9137) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9137-ctnna1-primary-antibodies-fc-testing-6.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband&reg; Flow Cytometry analysis of U-87 cells using anti-CTNNA1 antibody (PB9137). <br>Overlay histogram showing U-87 cells stained with PB9137 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTNNA1 Antibody (PB9137&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9137-ctnna1-primary-antibodies-if-testing-7.jpgAnti-alpha 1 Catenin/CTNNA1 Antibody Picoband&reg; IF analysis of CTNNA1 using anti-CTNNA1 antibody (PB9137). <br> CTNNA1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CTNNA1 Antibody (PB9137) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-laminin-picoband-trade-antibody-pb9142-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9142-laminin-primary-antibodies-wb-testing-1.jpgAnti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband&reg; Western blot analysis of Laminin using anti-Laminin antibody (PB9142). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human PC-3 whole cell lysates,<br> Lane 4: human Caco-2 whole cell lysates.<br> Lane 5: rat NRK whole cell lysates,<br> Lane 6: rat RH35 whole cell lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates,<br> Lane 8: mouse Hepa1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Laminin antigen affinity purified polyclonal antibody (Catalog # PB9142) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Laminin at approximately 220-250 kDa. The expected band size for Laminin is at 177 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9142-3-IHC-anti-laminin-picoband-antibody.jpgAnti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband&reg; IHC analysis of Laminin using anti-Laminin antibody (PB9142). <br> Laminin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9142-3_1.jpgAnti-Laminin/Lamc1/Lamc2/Lamc3 Antibody Picoband&reg; IHC analysis of Laminin using anti-Laminin antibody (PB9142). <br> Laminin was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Laminin Antibody (PB9142) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-muc4-picoband-trade-antibody-pb9147-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9147-2-WB-anti-muc4-picoband-antibody.jpgAnti-MUC4 Antibody Picoband&reg; Western blot analysis of MUC4 using anti-MUC4 antibody (PB9147). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat PC-12 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC4 antigen affinity purified polyclonal antibody (Catalog # PB9147) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC4 at approximately 234 kDa. The expected band size for MUC4 is at 232 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc9a1-picoband-trade-antibody-pb9151-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9151-1-WB-anti-slc9a1-nhe1-picoband-antibody.jpgAnti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband&reg; Western blot analysis of SLC9A1 using anti-SLC9A1 antibody (PB9151). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant human Sodium protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A1 antigen affinity purified polyclonal antibody (Catalog # PB9151) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A1 at approximately 37 kDa. The expected band size for SLC9A1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9151-2-WB-anti-slc9a1-nhe1-picoband-antibody.jpgAnti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband&reg; Western blot analysis of SLC9A1 using anti-SLC9A1 antibody (PB9151). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat PC-12 whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A1 antigen affinity purified polyclonal antibody (Catalog # PB9151) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A1 at approximately 91 kDa. The expected band size for SLC9A1 is at 91 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9151-3-IHC-anti-slc9a1-nhe1-picoband-antibody.jpgAnti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband&reg; IHC analysis of SLC9A1 using anti-SLC9A1 antibody (PB9151). <br> SLC9A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A1 Antibody (PB9151) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9151-4-IHC-anti-slc9a1-nhe1-picoband-antibody.jpgAnti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband&reg; IHC analysis of SLC9A1 using anti-SLC9A1 antibody (PB9151). <br> SLC9A1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A1 Antibody (PB9151) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9151-5-IHC-anti-slc9a1-nhe1-picoband-antibody.jpgAnti-Sodium/Hydrogen Exchanger 1/SLC9A1 Antibody Picoband&reg; IHC analysis of SLC9A1 using anti-SLC9A1 antibody (PB9151). <br> SLC9A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC9A1 Antibody (PB9151) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-perforin-picoband-trade-antibody-pb9155-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9155-perforin-primary-antibodies-wb-testing-1.jpgAnti-Perforin/PRF1 Antibody Picoband&reg;Western blot analysis of ADFP/Perforin using anti-ADFP/Perforin antibody (PB9155). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human HEL whole cell lysates,<br> Lane 3: human Daudi whole cell lysates,<br> Lane 4: human HUH7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADFP/Perforin antigen affinity purified polyclonal antibody (Catalog # PB9155) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADFP/Perforin at approximately 61 kDa. The expected band size for ADFP/Perforin is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-runx1-aml1-picoband-trade-antibody-pb9157-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9157-1_1.jpgAnti-RUNX1/AML1 Antibody Picoband&reg; Western blot analysis of RUNX1 using anti-RUNX1 antibody (PB9157). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HL-60 whole cell lysates&#44; <br> Lane 2: rat thymus tissue lysates&#44; <br> Lane 3: mouse thymus tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1 antigen affinity purified polyclonal antibody (Catalog # PB9157) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX1 at approximately 55KD. The expected band size for RUNX1 is at 49KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9157-2_1.jpgAnti-RUNX1/AML1 Antibody Picoband&reg; IHC analysis of RUNX1 using anti-RUNX1 antibody (PB9157). <br> RUNX1 was detected in paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX1 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9157-3_1.jpgAnti-RUNX1/AML1 Antibody Picoband&reg; IHC analysis of RUNX2 using anti-RUNX2 antibody (PB9157). <br> RUNX2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX2 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9157-4_1.jpgAnti-RUNX1/AML1 Antibody Picoband&reg; IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9157). <br> RUNX3 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX3 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9157-5.jpgAnti-RUNX1/AML1 Antibody Picoband&reg; IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9157). <br> RUNX3 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX3 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9157-6.jpgAnti-RUNX1/AML1 Antibody Picoband&reg; IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9157).<br> RUNX3 was detected in frozen section of rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX3 Antibody (PB9157) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sirt1-picoband-trade-antibody-pb9159-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9159-41420_2023_1432_fig2_html.pngAnti-SIRT1 Antibody Picoband&reg;Sirtuin 6 is the possible contributor to gender differences upon IRI. A Graphical representations show the relative abundance of Sirtuin 1-7 mRNA in different groups. ** P < 0.01, *** P < 0.001 versus sham controls in male group ( n = 5); † P < 0.05 versus sham controls in female group ( n = 5). B , C Representative western blot ( B ) and graphical representations of ( C ) Sirtuin 1-7 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus male group ( n = 5). D Representative micrographs showing the expression of Sirtuin 6 in different groups, as indicated. Paraffin-embedded kidney sections were stained with an antibody against Sirtuin 6. Arrows indicate positive staining. Scale bar, 50 μm. E Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. F – H Representative western blot ( F ) and graphical representations of ( G ) PGC-1α and ( H ) TOMM20 protein expression levels are shown. *** P < 0.001 versus male group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9159-41420_2023_1432_fig4_html.pngAnti-SIRT1 Antibody Picoband&reg;The expression of Sirtuin 6 was the key regulator for gender differences in rhabdomyolysis-induced AKI. A , B Representative western blot ( A ) and graphical representations of ( B ) Sirtuin 1-7 protein expression levels are shown. * P < 0.05, ** P < 0.01, *** P < 0.001 versus female group ( n = 5). C Representative micrographs show the expression of Sirtuin 6 in different groups, as indicated. Paraffin-embedded kidney sections were stained with an antibody against Sirtuin 6. Arrows indicate positive staining. Scale bar, 50 μm. D Representative micrographs showing the expression of PGC-1α and TOMM20 in different groups, as indicated. Frozen kidney sections were stained with an antibody against PGC-1α and TOMM20. Arrows indicate positive staining. Scale bar, 50 μm. E – G Representative western blot ( E ) and graphical representations of ( F ) PGC-1α and ( G ) TOMM20 protein expression levels are shown. * P < 0.05, *** P < 0.001 versus male group ( n = 5). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9159-41420_2023_1432_fig7_html.pngAnti-SIRT1 Antibody Picoband&reg;AR increases acetylation of PGC-1α by downregulating Sirtuin 6 expression. A – C Representative western blot ( A ) and graphical representations of ( B ) Sirtuin 1-7 and ( C ) PGC-1α protein expression levels are shown. * P < 0.05, ** P < 0.01 versus control group ( n = 3). HKC-8 cells were transfected with pcDNA3 or AR overexpression plasmid for 24 h. D Representative ChIP assay results showing the binding of AR to the Sirtuin 6 gene promoter region. HKC‐8 cells were incubated with DHT (10 μMol/L) or not for 24 h. Cell lysates were precipitated with an antibody against AR, histone H3, or nonimmune IgG, and the ChIP assay was performed for Sirtuin 6 gene promoters. Total diluted lysate was used as the total genomic input DNA. E Graphical representations show the relative abundance of Sirtuin 1-7 mRNA in different groups. ** P < 0.01 versus control group ( n = 3). F – I Representative western blot ( F ) and graphical representations of ( G ) Sirtuin 1-7, ( H ) PGC-1α and ( I ) AR protein expression levels are shown. HKC-8 cells were incubated with DHT (10 μMol/L) and transfected with AR-shRNA for 24 h. * P < 0.05, ** P < 0.01, *** P < 0.001 ( n = 3); † P < 0.05, †† P < 0.01, ††† P < 0.001 ( n = 3). J Representative graphs show the binding of PGC-1α with Sirtuin 6 or acetyl. HKC-8 cells were transfected with pcDNA3 or AR overexpression plasmid for 24 h. K Representative graphs show the binding of PGC-1α with acetyl, and the expression of PGC-1α in different groups, as indicated. HKC-8 cells were treated with DHT (10 μMol/L) and transfected with Sirtuin 6 overexpression plasmid for 24 h. L Representative graphs show the binding of PGC-1α with acetyl, and the protein levels of AR in nuclear fractions in different groups, as indicated. HKC-8 cells were treated with DHT (10 μMol/L) for 24 h. M Representative graphs show the binding of PGC-1α with acetyl, and the protein levels of AR in nuclear fractions in sham control and IRI group in male mice, as indicated. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-023-01432-y'>37185276</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9159-sirt1-primary-antibodies-wb-testing-1.jpgAnti-SIRT1 Antibody Picoband&reg; Western blot analysis of SIRT1 using anti-SIRT1 antibody (PB9159). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: huamn SiHa whole cell lysates, <br> Lane 4: huamn Daudi whole cell lysates, <br> Lane 5: huamn K562 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT1 antigen affinity purified polyclonal antibody (Catalog # PB9159) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT1 at approximately 120 kDa. The expected band size for SIRT1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9159-sirt1-primary-antibodies-if-testing-2.jpgAnti-SIRT1 Antibody Picoband&reg; IF analysis of SIRT1 using anti-SIRT1 antibody (PB9159). <br> SIRT1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SIRT1 Antibody (PB9159) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd11b-picoband-trade-antibody-pb9140-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9140-1-WB-anti-cd11b-picoband-antibody.jpgAnti-CD11b/ITGAM Antibody Picoband&reg; Western blot analysis of CD11b using anti-CD11b antibody (PB9140). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human CD11B protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody (Catalog # PB9140) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11b at approximately 45 kDa. The expected band size for CD11b is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9140-2-IHC-anti-cd11b-picoband-antibody.jpgAnti-CD11b/ITGAM Antibody Picoband&reg; IHC analysis of CD11b using anti-CD11b antibody (PB9140). <br> CD11b was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD11b Antibody (PB9140) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9140-3-IHC-anti-cd11b-picoband-antibody.jpgAnti-CD11b/ITGAM Antibody Picoband&reg; IHC analysis of CD11b using anti-CD11b antibody (PB9140). <br> CD11b was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD11b Antibody (PB9140) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9140-4-IHC-anti-cd11b-picoband-antibody.jpgAnti-CD11b/ITGAM Antibody Picoband&reg; IHC analysis of CD11b using anti-CD11b antibody (PB9140). <br> CD11b was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD11b Antibody (PB9140) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9140-5.pngAnti-CD11b/ITGAM Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-CD11b antibody (PB9140). <br> Overlay histogram showing THP-1 cells stained with PB9140 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD11b Antibody (PB9140&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/picokine-elisa-kits/human-il-13-picokine-trade-elisa-kit-ek0424-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0424_1.pngHuman IL-13/Interleukin-13 ELISA Kit PicoKine®Human IL-13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-kininogen-1-kng1-elisa-kit-ek1154-boster.html2026-03-24T05:04:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1154_1.pngHuman Kininogen-1 / KNG1 / HMW Kininogen ELISA Kit PicoKine®Human Kininogen-1/KNG1 ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sp-d-picokine-trade-elisa-kit-ek1171-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1171_1.jpgHuman SP-D / Surfactant protein D ELISA Kit PicoKine®Human SP-D PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-neuropilin-1-picokine-trade-elisa-kit-ek1353-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1353_1.jpgHuman Neuropilin-1 ELISA Kit PicoKine®Human Neuropilin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tfpi-picokine-trade-elisa-kit-ek1355-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1355.pngMouse TFPI ELISA Kit PicoKine®Mouse TFPI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-s100a9-picokine-trade-elisa-kit-ek1152-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1152.pngMouse S100A9 ELISA Kit PicoKine®Mouse S100A9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-syndecan-4-sdc4-picokine-trade-elisa-kit-ek1356-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1356.pngHuman Syndecan-4/SDC4 ELISA Kit PicoKine®Human Syndecan-4/SDC4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-biglycan-picokine-trade-elisa-kit-ek1357-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1357.pngHuman Biglycan ELISA Kit PicoKine®Human Biglycan PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tfpi2-picokine-trade-elisa-kit-ek1358-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1358_1.pngHuman TFPI2 ELISA Kit PicoKine®Human TFPI2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ceacam1-picokine-trade-elisa-kit-ek1361-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1361_1.jpgHuman CEACAM1/Cd66A ELISA Kit PicoKine®Human CEACAM1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-37-il1f7-picokine-trade-elisa-kit-ek1363-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1363_2.pngHuman IL-37/IL-1F7 ELISA Kit PicoKine®Human IL-37/IL1F7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-17d-picokine-trade-elisa-kit-ek0792-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0792.pngMouse IL-17D ELISA Kit PicoKine®Mouse IL-17D PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-34-picokine-trade-elisa-kit-ek1365-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1365.pngHuman IL-34/Interleukin-34 ELISA Kit PicoKine®Human IL-34 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-34-picokine-trade-elisa-kit-ek1366-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1366.pngMouse IL-34/Interleukin-34 ELISA Kit PicoKine®Mouse IL-34 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tissue-factor-f3-picokine-trade-elisa-kit-ek1367-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1367_1.pngMouse Tissue Factor/F3 ELISA Kit PicoKine®Mouse Tissue Factor/F3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-pltp-picokine-trade-elisa-kit-ek1368-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1368_1.jpgMouse PLTP ELISA Kit PicoKine®Mouse PLTP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-hemojuvelin-picokine-trade-elisa-kit-ek1370-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1370_1.pngMouse Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®Mouse Hemojuvelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adamts4-picokine-trade-elisa-kit-ek1372-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1372_1.jpgHuman ADAMTS4 ELISA Kit PicoKine®Human ADAMTS4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cxcl3-picokine-trade-elisa-kit-ek1373-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1373.pngRat CXCL3 ELISA Kit PicoKine®Rat CXCL3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-angptl3-picokine-trade-elisa-kit-ek1374-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1374_1.jpgHuman ANGPTL3 ELISA Kit PicoKine®Human ANGPTL3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-igf2r-picokine-trade-elisa-kit-ek1326-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1326_1.pngHuman IGF2R/IGF-II R ELISA Kit PicoKine®Human IGF2R PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-endostatin-picokine-trade-elisa-kit-ek1376-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1376.pngMouse Endostatin ELISA Kit PicoKine®Mouse Endostatin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-neuregulin-1-nrg1-beta1-picokine-trade-elisa-kit-ek1378-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1378.pngHuman Neuregulin-1/NRG1-Beta1 ELISA Kit PicoKine®Human Neuregulin-1/NRG1-Beta1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-timp-1-picokine-trade-elisa-kit-ek0521-boster.html2026-03-24T05:04:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0521.jpgMouse TIMP-1 ELISA Kit PicoKine®Mouse TIMP-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-mbp-c-mbl2-picokine-trade-elisa-kit-ek0805-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0805_1.jpgHuman MBL2/MPB-C ELISA Kit PicoKine®Human MBP-C/MBL2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mbl2-picokine-trade-elisa-kit-ek0806-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0806_1.jpgMouse MBL2/MPB-C ELISA Kit PicoKine®Mouse MBL2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atm-antibody-rp1040-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9163-1-RP1040-WB-anti-atm-antibody.jpgAnti-Serine-protein kinase ATM ATM Antibody Picoband&reg;All lanes: Anti ATM (RP1040) at 0.5ug/ml<br>WB: Recombinant Human ATM Protein 0.5ng<br>Predicted bind size: 60KD<br>Observed bind size: 60KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-b-raf-picoband-trade-antibody-pb9164-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-wb-testing-1.jpgAnti-B Raf/BRAF Antibody Picoband&reg; Western blot analysis of B Raf/BRAF using anti-B Raf/BRAF antibody (PB9164). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B Raf/BRAF antigen affinity purified polyclonal antibody (Catalog # PB9164) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B Raf/BRAF at approximately 84 kDa. The expected band size for B Raf/BRAF is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-ihc-testing-2.jpgAnti-B Raf/BRAF Antibody Picoband&reg; IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody (PB9164). <br> B Raf/BRAF was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-B Raf/BRAF Antibody (PB9164) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-ihc-testing-3.jpgAnti-B Raf/BRAF Antibody Picoband&reg; IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody (PB9164). <br> B Raf/BRAF was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-B Raf/BRAF Antibody (PB9164) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-ihc-testing-4.jpgAnti-B Raf/BRAF Antibody Picoband&reg; IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody (PB9164). <br> B Raf/BRAF was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-B Raf/BRAF Antibody (PB9164) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-ihc-testing-5.jpgAnti-B Raf/BRAF Antibody Picoband&reg; IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody (PB9164). <br> B Raf/BRAF was detected in a paraffin-embedded section of human differentiated adenocarcinoma of the rectum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-B Raf/BRAF Antibody (PB9164) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-ihc-testing-6.jpgAnti-B Raf/BRAF Antibody Picoband&reg; IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody (PB9164). <br> B Raf/BRAF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-B Raf/BRAF Antibody (PB9164) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-fcm-testing-8.pngAnti-B Raf/BRAF Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-B Raf/BRAF antibody (PB9164). <br>Overlay histogram showing U20S cells stained with PB9164 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B Raf/BRAF Antibody (PB9164, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9164-braf-primary-antibodies-ihc-testing-7.jpgAnti-B Raf/BRAF Antibody Picoband&reg; IHC analysis of B Raf/BRAF using anti-B Raf/BRAF antibody (PB9164). <br> B Raf/BRAF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-B Raf/BRAF Antibody (PB9164) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caveolin-1-picoband-trade-antibody-pb9165-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-cav1-primary-antibodies-wb-testing-1.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human placenta tissue lysates, <br> Lane 4: human A431 whole cell lysates, <br> Lane 5: human HL-60 whole cell lysates, <br> Lane 6: rat ovary tissue lysates, <br> Lane 7: rat heart tissue lysates, <br> Lane 8: mouse ovary tissue lysates, <br> Lane 9: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-1/CAV1 antigen affinity purified polyclonal antibody (Catalog # PB9165) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-1/CAV1 at approximately 22 kDa. The expected band size for Caveolin-1/CAV1 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-12860_2020_324_fig4_html.pngAnti-Caveolin-1/CAV1 Antibody Picoband&reg;IMFlnc1 was associated with adipogenesis. a The expression profile of IMFlnc1 in different tissues by qRT-PCR. b The expression level of IMFlnc1 and PPARgama during porcine intramuscular preadipocyte differentiation by qRT-PCR. c The coding probability of IMFlnc1, ADNCR and CAV-1 were analyzed by Coding Potential Calculator 2 (CPC2) program. d IMFlnc1 siRNA could downregulate expression of IMFlnc1, CAV-1 and PPARgama by qRT-PCR. e The effect of IMFlnc1 siRNA on CAV-1 protein expression by Western blot. f Inhibition of IMFlnc1 inhibited adipogenesis by Oil Red O staining <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12860-020-00324-8'>33148167</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-12860_2020_324_fig5_html.pngAnti-Caveolin-1/CAV1 Antibody Picoband&reg;IMFlnc1 regulates CAV-1 expression by sponging miR-199a-5p. a Detection of IMFlnc1 in porcine intramuscular adipocyte by RNA fluorescence in situ hybridization (RNA-FISH). Red represents FISH probes of IMFlnc1. Nuclei are counterstained with DAPI (blue). Scale bar, 50 μm. b The schematic diagram shows the sequences of IMFlnc1 and CAV-1 with miR-199a-5p, inculding wild-type (Wt) and mutant type (Mut). c The binding site of miR-199a-5p at CAV-1 3’UTR (green) are evolutionarily conserved across species. d The correlation analysis of IMFlnc1 expression level and CAV-1 mRNA level in 18 porcine LD muscle tissues. e IMFlnc1 acted as the target of miR-199a-5p. f CAV-1 acted as the target of miR-199a-5p. g IMFlnc1 acted as the sponge of miR-199a-5p. IMFlnc1 increases CAV-1 expression ( h ) and adipogenesis ( i ) in a miR-199a-5p dependent manner <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12860-020-00324-8'>33148167</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-cav1-primary-antibodies-ihc-testing-2.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165). <br> Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caveolin-1/CAV1 Antibody (PB9165) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-cav1-primary-antibodies-ihc-testing-3.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165). <br> Caveolin-1/CAV1 was detected in a paraffin-embedded section of human meningeoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caveolin-1/CAV1 Antibody (PB9165) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-cav1-primary-antibodies-ihc-testing-4.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165). <br> Caveolin-1/CAV1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caveolin-1/CAV1 Antibody (PB9165) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-cav1-primary-antibodies-ihc-f-testing-5.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165). <br> Caveolin-1/CAV1 was detected in a frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caveolin-1/CAV1 Antibody (PB9165) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-cav1-primary-antibodies-if-testing-6.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PB9165). <br> Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Caveolin-1/CAV1 Antibody (PB9165) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9165-cav1-primary-antibodies-fcm-testing-7.jpgAnti-Caveolin-1/CAV1 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-Caveolin-1/CAV1 antibody (PB9165). <br> Overlay histogram showing U87 cells stained with PB9165 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1/CAV1 Antibody (PB9165, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caveolin-2-picoband-trade-antibody-pb9166-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9166-caveolin-2-primary-antibodies-wb-testing-1.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg; Western blot analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HT1080 whole cell lysates, <br> Lane 2: human U20S whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 4: human Hela whole cell lysates,<br> Lane 5: human A549 whole cell lysates,<br> Lane 6: rat lung tissue lysates,<br> Lane 7: mouse lung tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-2 antigen affinity purified polyclonal antibody (Catalog # PB9166) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-2 at approximately 21KD. The expected band size for Caveolin-2 is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9166-caveolin-2-primary-antibodies-ihc-testing-2.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg; IHC analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166). <br> Caveolin-2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9166-caveolin-2-primary-antibodies-ihc-testing-3.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg; IHC analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166). <br> Caveolin-2 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9166-caveolin-2-primary-antibodies-if-testing-4.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg; IF analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166). <br> Caveolin-2 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9166-caveolin-2-primary-antibodies-if-testing-5.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg; IF analysis of Caveolin-2 using anti-Caveolin-2 antibody (PB9166). <br> Caveolin-2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Caveolin-2 Antibody (PB9166) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9166-caveolin-2-primary-antibodies-fcm-testing-6.jpgAnti-Caveolin-2/CAV2 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-Caveolin-2 antibody (PB9166).<br>Overlay histogram showing A549 cells stained with PB9166 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-2 Antibody (PB9166,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cbs-antibody-rp1041-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1041-cbs-primary-antibodies-wb-testing-1.jpgAnti-Cystathionine beta-synthase CBS Antibody Picoband&reg; Western blot analysis of CBS using anti-CBS antibody (RP1041). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: rat liver tissue lysates,<br> Lane 5: rat pancrease tissue lysates,<br> Lane 6: mouse liver tissue lysates,<br> Lane 7: mouse pancrease tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBS antigen affinity purified polyclonal antibody (Catalog # RP1041) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBS at approximately 65 kDa. The expected band size for CBS is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1041-cbs-primary-antibodies-ihc-testing-2.jpgAnti-Cystathionine beta-synthase CBS Antibody Picoband&reg; IHC analysis of CBS using anti-CBS antibody (RP1041). <br> CBS was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CBS Antibody (RP1041) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd79a-picoband-trade-antibody-pb9168-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9168-cd79a-primary-antibodies-wb-testing-1.jpgAnti-CD79a Antibody Picoband&reg; Western blot analysis of CD79A using anti-CD79A antibody (PB9387). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates, <br> Lane 2: human Ramos whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79A antigen affinity purified polyclonal antibody (Catalog # PB9387) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD79A at approximately 44 kDa. The expected band size for CD79A is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9168-cd79a-primary-antibodies-ihc-testing-2.jpgAnti-CD79a Antibody Picoband&reg; IHC analysis of CD79A using anti-CD79A antibody (PB9168). <br> CD79A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD79A Antibody (PB9168) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9168-cd79a-primary-antibodies-fcm-testing-4_1.jpgAnti-CD79a Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-CD79A antibody (PB9168). <br> Overlay histogram showing Jurkat cells stained with PB9168 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD79A Antibody (PB9168, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9168-cd79a-primary-antibodies-if-testing-3.jpgAnti-CD79a Antibody Picoband&reg; IF analysis of CD79A using anti-CD79A antibody (PB9168). <br> CD79A was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD79A Antibody (PB9168) overnight at 4°C. DyLight®550 conjugated goat anti-rabbit IgG (BA1135) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9168-cd79a-primary-antibodies-fcm-testing-5.jpgAnti-CD79a Antibody Picoband&reg; Flow Cytometry analysis of Ramos cells using anti-CD79A antibody (PB9168). <br> Overlay histogram showing Ramos cells stained with PB9168 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD79A Antibody (PB9168, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd79b-picoband-trade-antibody-pb9169-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9169-cd79b-primary-antibodies-wb-testing-1.jpgAnti-CD79b Antibody Picoband&reg;Western blot analysis of CD79b using anti-CD79b antibody (PB9169). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human Daudi whole cell lysates,<br> Lane 3: human Ramos whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD79b antigen affinity purified polyclonal antibody (PB9169) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD79b at approximately 30-40 kDa. The expected band size for CD79b is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9169-cd79b-primary-antibodies-ihc-testing-1.jpgAnti-CD79b Antibody Picoband&reg;IHC analysis of CD79b using anti-CD79b antibody (PB9169). <br> CD79b was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD79b Antibody (PB9169) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9169-cd79b-primary-antibodies-if-testing-1.jpgAnti-CD79b Antibody Picoband&reg;IF analysis of CD79b using anti-CD79b antibody (PB9169). <br> CD79b was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD79b Antibody (PB9169) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9169-cd79b-primary-antibodies-fcm-testing-1.jpgAnti-CD79b Antibody Picoband&reg;Flow Cytometry analysis of Raji cells using anti-CD79b antibody (PB9169). <br> Overlay histogram showing Raji cells stained with PB9169 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD79b Antibody (PB9169, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd82-picoband-trade-antibody-pb9170-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9170-cd82-primary-antibodies-wb-testing-1_1.jpgAnti-CD82 Antibody Picoband&reg; Western blot analysis of CD82 using anti-CD82 antibody (PB9170). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates, <br> Lane 2: human Raji whole cell lysates, <br> Lane 3: human U-87MG whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD82 antigen affinity purified polyclonal antibody (Catalog # PB9170) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD82 at approximately 30-36 kDa. The expected band size for CD82 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9170-cd82-primary-antibodies-ihc-testing-2_1.jpgAnti-CD82 Antibody Picoband&reg; IHC analysis of CD82 using anti-CD82 antibody (PB9170). <br> CD82 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD82 Antibody (PB9170) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9170-cd82-primary-antibodies-ihc-testing-3_1.jpgAnti-CD82 Antibody Picoband&reg; IHC analysis of CD82 using anti-CD82 antibody (PB9170). <br> CD82 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD82 Antibody (PB9170) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cebp-beta-picoband-trade-antibody-pb9171-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9171-cebpb-primary-antibodies-wb-testing-1.jpgAnti-CEBP Beta/CEBPB Antibody Picoband&reg; Western blot analysis of CEBP Beta using anti-CEBP Beta antibody (PB9171). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human U87 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBP Beta antigen affinity purified polyclonal antibody (Catalog # PB9171) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CEBP Beta at approximately 42, 46 kDa. The expected band size for CEBP Beta is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9171-cebpb-primary-antibodies-ihc-testing-2.jpgAnti-CEBP Beta/CEBPB Antibody Picoband&reg; IHC analysis of CEBP Beta using anti-CEBP Beta antibody (PB9171). <br> CEBP Beta was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CEBP Beta Antibody (PB9171) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9171-cebpb-primary-antibodies-ihc-testing-3.jpgAnti-CEBP Beta/CEBPB Antibody Picoband&reg; IHC analysis of CEBP Beta using anti-CEBP Beta antibody (PB9171). <br> CEBP Beta was detected in a paraffin-embedded section of human squamous metaplasia of renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CEBP Beta Antibody (PB9171) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9171-cebpb-primary-antibodies-if-testing-4_1.jpgAnti-CEBP Beta/CEBPB Antibody Picoband&reg; IF analysis of CEBP Beta using anti-CEBP Beta antibody (PB9171). <br> CEBP Beta was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CEBP Beta Antibody (PB9171) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9171-cebpb-primary-antibodies-fcm-testing-5.jpgAnti-CEBP Beta/CEBPB Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-CEBP Beta antibody (PB9171). <br> Overlay histogram showing A431 cells stained with PB9171 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CEBP Beta Antibody (PB9171, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-claudin-2-antibody-rp1042-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1042-1-WB-anti-claudin-2-antibody.jpgAnti-Claudin 2/CLDN2 Antibody Picoband&reg;Anti-Claudin 2 antibody&#44; RP1042&#44; Western blotting<br>All lanes: Anti Claudin 2 (RP1042) at 0.5ug/ml<br>Lane 1: Rat Kidney Tissue Lysate at 50ug<br>Lane 2: Rat Liver Tissue Lysate at 50ug<br>Predicted bind size: 25KD<br>Observed bind size: 25KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1042-2-IHC-anti-claudin-2-antibody.jpgAnti-Claudin 2/CLDN2 Antibody Picoband&reg;Anti-Claudin 2 antibody&#44; RP1042&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1042-claudin-2-primary-antibody-wb-testing-1_1.pngAnti-Claudin 2/CLDN2 Antibody Picoband&reg; Western blot analysis of Claudin 2 using anti-Claudin 2 antibody (RP1042). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Normal group-rat colon tissue lysates, <br> Lane 2: Model group-rat colon tissue lysates, <br> Lane 3: Triditional Chinese medicine treatment (low dose)-rat colon tissue lysates, <br> Lane 4: Triditional Chinese medicine treatment (medium dose)-rat colon tissue lysates, <br> Lane 5: Triditional Chinese medicine treatment(high dose)-rat colon tissue lysates, <br> Lane 6: Western medicine treatment-rat colon tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin 2 antigen affinity purified polyclonal antibody (Catalog # RP1042) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Claudin 2 at approximately 20 kDa. The expected band size for Claudin 2 is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-claudin-5-antibody-rp1043-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1043-2-WB-anti-claudin-5-antibody.jpgAnti-Claudin 5/CLDN5 Antibody Picoband&reg;Anti-Claudin 5 Picoband antibody&#44; RP1043&#44; Western blotting<br>All lanes: Anti Claudin-5 (RP1043) at 0.5ug/ml<br>WB: Rat Liver Tissue Lysate at 50ug<br>Predicted bind size: 23KD<br>Observed bind size: 23KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-decorin-picoband-trade-antibody-pb9174-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-decorin-primary-antibodies-wb-testing-1.jpgAnti-Decorin/DCN Antibody Picoband&reg;Western blot analysis of Decorin using anti-Decorin antibody (PB9174). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human SW620 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat RH35 whole cell lysates,<br> Lane 7: mouse HEPA1-6 whole cell lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Decorin antigen affinity purified polyclonal antibody (PB9174) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Decorin at approximately 70 kDa. The expected band size for Decorin is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-decorin-primary-antibodies-ihc-testing-1.jpgAnti-Decorin/DCN Antibody Picoband&reg;IHC analysis of Decorin using anti-Decorin antibody (PB9174). <br>Decorin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Decorin Antibody (PB9174) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-41598_2017_article_bfsrep44473_fig5_html.jpgAnti-Decorin/DCN Antibody Picoband&reg;The AKT inhibitor MK2206 abolished the effects induced by overexpression of decorin. ( A ) The tube formation test; the photographs were taken at a magnification of 100×. ( B ) The cell wound healing test; the photographs were taken at a magnification of 100×. ( C ) The apoptosis assay. ( D ) CCK8 assessment. ( E , F ) The expression of VEGF, Bcl2, and Bax and the phosphorylation of AKT and AP 1. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. # p < 0.05, ## p < 0.01, compared to Con. & p < 0.05, && p < 0.01, compared to HG + GFP (HG). $ p < 0.05, $$ p < 0.01, compared to HG + DCN. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep44473'>28290552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-41598_2017_article_bfsrep44473_fig6_html.jpgAnti-Decorin/DCN Antibody Picoband&reg;The IGF1R antibody (αIGF1R) blocked the effects induced by overexpression of decorin. ( A , B ) The tube formation test; the photographs were taken at a magnification of 100×. ( C , D ) The cell wound healing test; the photographs were taken at a magnification of 100×. ( E , F ) The apoptosis assay. ( G ) CCK8 assessment. ( H , I ) The expression of VEGF, Bcl2, and Bax and the phosphorylation of AKT and AP 1. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. # p < 0.05, ## p < 0.01, compared to Con. & p < 0.05, && p < 0.01, compared to HG + GFP. $ p < 0.05, $$ p < 0.01, compared to HG + DCN. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep44473'>28290552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-41598_2017_article_bfsrep44473_fig4_html.jpgAnti-Decorin/DCN Antibody Picoband&reg;Overexpression of decorin activated the IGF1R-AKT-AP-1 pathway. ( A , B ) The phosphorylation of AKT was decreased by HG treatment in a time-dependent manner. ( C – E ) The expression of IGF1R, Bcl2, and Bax and the phosphorylation of AKT. ( F , G ) The phosphorylation of AP-1. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. # p < 0.05, compared to Con. & p < 0.05, && p < 0.01, compared to HG + GFP. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep44473'>28290552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-41598_2017_article_bfsrep44473_fig3_html.jpgAnti-Decorin/DCN Antibody Picoband&reg;Overexpression of decorin ameliorated the angiogenesis impaired by high glucose (HG). ( A – C ) The expression of decorin and VEGF. ( D , E ) The tube formation test; the photographs were taken at a magnification of 100×. ( F , G ) The cell wound healing test; the photographs were taken in a magnification of 100×. ( H , I ) The apoptosis rate analyzed by an Annexin V–FITC kit through flow cytometry. ( J ) The proliferative ability assessed with a CCK8 assay. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep44473'>28290552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-41598_2017_article_bfsrep44473_fig2_html.jpgAnti-Decorin/DCN Antibody Picoband&reg;Overexpression of decorin increased angiogenesis in the diabetic hearts. ( A – C ) The expression of decorin and VEGF. ( D ) The VEGF concentration in the plasma. ( E ) The capillary density using immunochemistry with a CD31 antibody. The photographs were taken at a magnification of 100× and zoomed at a magnification of 200×. The arrows show the capillary stained with the CD31 antibody. ( F ) The number of vessels counted from the immunochemistry staining. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep44473'>28290552</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9174-41598_2017_article_bfsrep44473_fig1_html.jpgAnti-Decorin/DCN Antibody Picoband&reg;Overexpression of decorin improved cardiac function in diabetic cardiomyopathy. ( A ) The oral glucose tolerance test (OGTT). ( B ) The left ventricular ejection fraction (EF) evaluated by echocardiography. ( C ) The fraction shortening (FS) evaluated by echocardiography. ( D , E ) The left ventricular wall thickness (including the interventricular septum (IVS) and the posterior wall (PW)) and the internal dimension of the left ventricle (LVID). ( F – H ) The hemodynamic function was evaluated by the cardiac catheter system, including the left ventricular end-diastolic pressure (LVEDP), as well as the Dp/dt maximum and minimum. All data are presented as the mean ± SEM. *p < 0.05; **p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep44473'>28290552</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dhfr-picoband-trade-antibody-pb9175-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9175-dhfr-primary-antibodies-wb-testing-1_1.jpgAnti-Dihydrofolate reductase (DHFR) Antibody Picoband&reg; Western blot analysis of DHFR using anti-DHFR antibody (PB9175). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: rat kidney tissue lysates, <br> Lane 5: mouse liver tissue lysates, <br> Lane 6: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHFR antigen affinity purified polyclonal antibody (Catalog # PB9175) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DHFR at approximately 21 kDa. The expected band size for DHFR is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9175-dhfr-primary-antibodies-ihc-testing-2.jpgAnti-Dihydrofolate reductase (DHFR) Antibody Picoband&reg; IHC analysis of DHFR using anti-DHFR antibody (PB9175). <br> DHFR was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHFR Antibody (PB9175) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9175-dhfr-primary-antibodies-ihc-testing-3.jpgAnti-Dihydrofolate reductase (DHFR) Antibody Picoband&reg; IHC analysis of DHFR using anti-DHFR antibody (PB9175). <br> DHFR was detected in a paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHFR Antibody (PB9175) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9175-dhfr-primary-antibodies-ihc-testing-4.jpgAnti-Dihydrofolate reductase (DHFR) Antibody Picoband&reg; IHC analysis of DHFR using anti-DHFR antibody (PB9175). <br> DHFR was detected in a paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DHFR Antibody (PB9175) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9175-dhfr-primary-antibodies-if-testing-5_1.jpgAnti-Dihydrofolate reductase (DHFR) Antibody Picoband&reg; IF analysis of DHFR using anti-DHFR antibody (PB9175). <br> DHFR was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-DHFR Antibody (PB9175) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9175-dhfr-primary-antibodies-fcm-testing-6.jpgAnti-Dihydrofolate reductase (DHFR) Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-DHFR antibody (PB9175). <br> Overlay histogram showing PC-3 cells stained with PB9175 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DHFR Antibody (PB9175, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dicer-antibody-rp1044-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1044-dicer-primary-antibodies-fcm-testing-5.jpgAnti-Dicer/DICER1 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-Dicer/DICER1 antibody (RP1044).<br>Overlay histogram showing MCF-7 cells stained with RP1044 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dicer/DICER1 Antibody (RP1044,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1044-dicer-primary-antibodies-wb-testing-1.jpgAnti-Dicer/DICER1 Antibody Picoband&reg; Western blot analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody (RP1044). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dicer/DICER1 antigen affinity purified polyclonal antibody (Catalog # RP1044) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dicer/DICER1 at approximately 250 kDa. The expected band size for Dicer/DICER1 is at 219 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1044-dicer-primary-antibodies-ihc-testing-2.jpgAnti-Dicer/DICER1 Antibody Picoband&reg; IHC analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody (RP1044). <br> Dicer/DICER1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dicer/DICER1 Antibody (RP1044) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1044-dicer-primary-antibodies-ihc-testing-3.jpgAnti-Dicer/DICER1 Antibody Picoband&reg; IHC analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody (RP1044). <br> Dicer/DICER1 was detected in a paraffin-embedded section of human penis squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dicer/DICER1 Antibody (RP1044) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1044-dicer-primary-antibodies-ihc-testing-4.jpgAnti-Dicer/DICER1 Antibody Picoband&reg; IHC analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody (RP1044). <br> Dicer/DICER1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Dicer/DICER1 Antibody (RP1044) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kat3b-p300-picoband-trade-antibody-pb9178-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9178-1-WB-anti-kat3b-p300-picoband-antibody.jpgAnti-KAT3B/p300/EP300 Antibody Picoband&reg; Western blot analysis of KAT3B/p300 using anti-KAT3B/p300 antibody (PB9178). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> lane 1: recombinant human kat3b/p300 protein 0.5ng. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/p300 antigen affinity purified polyclonal antibody (Catalog # PB9178) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/p300 at approximately 50KD. The expected band size for KAT3B/p300 is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9178-2_1.jpgAnti-KAT3B/p300/EP300 Antibody Picoband&reg; Western blot analysis of KAT3B/p300 using anti-KAT3B/p300 antibody (PB9178). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human COLO-320 whole cell lysates&#44; <br> Lane 2: rat PC-12 whole cell lysates&#44; <br> Lane 3: mouse NIH3T3 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/p3008 antigen affinity purified polyclonal antibody (Catalog # PB9178) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/p300 at approximately 300KD. The expected band size for KAT3B/p300 is at 264KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9178-3-IHC-anti-kat3b-p300-picoband-antibody.jpgAnti-KAT3B/p300/EP300 Antibody Picoband&reg; IHC analysis of KAT3B/p300 using anti-KAT3B/p300 antibody (PB9178). <br> KAT3B/p300 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KAT3B/p300 Antibody (PB9178) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9178-4-IHC-anti-kat3b-p300-picoband-antibody.jpgAnti-KAT3B/p300/EP300 Antibody Picoband&reg; IHC analysis of KAT3B/p300 using anti-KAT3B/p300 antibody (PB9178). <br> KAT3B/p300 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KAT3B/p300 Antibody (PB9178) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9178-5_1-IHC-anti-kat3b-p300-picoband-antibody.jpgAnti-KAT3B/p300/EP300 Antibody Picoband&reg; IHC analysis of KAT3B/p300 using anti-KAT3B/p300 antibody (PB9178). <br> KAT3B/p300 was detected in paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KAT3B/p300 Antibody (PB9178) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-epb41l1-picoband-trade-antibody-pb9179-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9179-epb41l1-primary-antibodies-wb-testing-1.jpgAnti-EPB41L1 Antibody Picoband&reg; Western blot analysis of EPB41L1 using anti-EPB41L1 antibody (PB9179). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human Caco-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPB41L1 antigen affinity purified polyclonal antibody (Catalog # PB9179) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPB41L1 at approximately 100-110 kDa. The expected band size for EPB41L1 is at 99 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fhit-picoband-trade-antibody-pb9181-boster.html2026-03-24T05:04:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9181-fhit-primary-antibodies-wb-testing-1.jpgAnti-FHIT Antibody Picoband&reg; Western blot analysis of FHIT using anti-FHIT antibody (PB9181). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHIT antigen affinity purified polyclonal antibody (Catalog # PB9181) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHIT at approximately 17 kDa. The expected band size for FHIT is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9181-2-ihc-anti-fhit-picoband-antibody.jpgAnti-FHIT Antibody Picoband&reg; IHC analysis of FHIT using anti-FHIT antibody (PB9181). <br> FHIT was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FHIT Antibody (PB9181) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9181-fhit-primary-antibodies-fcm-testing-4.jpgAnti-FHIT Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-FHIT antibody (PB9181).<br>Overlay histogram showing U20S cells stained with PB9181 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FHIT Antibody (PB9181,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9181-fhit-primary-antibodies-if-testing-3.jpgAnti-FHIT Antibody Picoband&reg; IF analysis of FHIT using anti-FHIT antibody (PB9181). <br> FHIT was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-FHIT Antibody (PB9181) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-furin-picoband-trade-antibody-pb9182-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9182-1-WB-anti-furin-picoband-antibody.jpgAnti-Furin Antibody Picoband&reg; Western blot analysis of Furin using anti-Furin antibody (PB9182). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human Furin protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Furin antigen affinity purified polyclonal antibody (Catalog # PB9182) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Furin at approximately 40 kDa. The expected band size for Furin is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9182-2-WB-anti-furin-picoband-antibody.jpgAnti-Furin Antibody Picoband&reg; Western blot analysis of Furin using anti-Furin antibody (PB9182). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human Colo320 whole cell lysates, <br> Lane 4: human SW620 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Furin antigen affinity purified polyclonal antibody (Catalog # PB9182) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Furin at approximately 87 kDa. The expected band size for Furin is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gad67-picoband-trade-antibody-pb9183-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9183-gad67-primary-antibodies-wb-testing-1_1.jpgAnti-GAD67/GAD1 Antibody Picoband&reg; Western blot analysis of GAD67/GAD1 using anti-GAD67/GAD1 antibody (PB9183). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAD67/GAD1 antigen affinity purified polyclonal antibody (Catalog # PB9183) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAD67/GAD1 at approximately 67 kDa. The expected band size for GAD67/GAD1 is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gst3-gst-pi-picoband-trade-antibody-pb9184-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9184-gstp1-primary-amtibodies-wb-testing-1.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg;Western blot analysis of GST3 using anti-GST3 antibody (PB9184).<br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br> Lane 1: huamn MDA-MB-231 whole cell lysates,<br> Lane 2: human MDA-MB-231 whole cell lysates,<br> Lane 3: human HCC1937 whole cell lysates,<br> Lane 4: human HCC1937 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3 antigen affinity purified polyclonal antibody (PB9184) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GST3 at approximately 23 kDa. The expected band size for GST3 is at 23 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9184-gst3-primary-antibodies-wb-testing-1.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; Western blot analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3/GST pi antigen affinity purified polyclonal antibody (Catalog # PB9184) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST3/GST pi at approximately 23 kDa. The expected band size for GST3/GST pi is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9184-gst3-primary-antibodies-fcm-testing-5.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-GST3/GST pi antibody (PB9184). <br> Overlay histogram showing THP-1 cells stained with PB9184 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GST3/GST pi Antibody (PB9184, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9184-2-ihc-anti-gst3-gst-pi-picoband-antibody.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; IHC analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184). <br> GST3/GST pi was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GST3/GST pi Antibody (PB9184) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9184-3-ihc-anti-gst3-gst-pi-picoband-antibody.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; IHC analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184). <br> GST3/GST pi was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GST3/GST pi Antibody (PB9184) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9184-4_1.jpgAnti-GST3/GST pi/GSTP1 Antibody Picoband&reg; IF analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184). <br> GST3/GST pi was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GST3/GST pi Antibody (PB9184) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gamma-catenin-picoband-trade-antibody-pb9185-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-8_1.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-gamma Catenin antibody (PB9185).<br>Overlay histogram showing A431 cells stained with PB9185 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-gamma Catenin Antibody (PB9185,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-gamma-catenin-primary-antibodies-wb-testing-1.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; Western blot analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human T47D whole cell lysates, <br> Lane 4: rat heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Catenin antigen affinity purified polyclonal antibody (Catalog # PB9185) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Catenin at approximately 82 kDa. The expected band size for gamma Catenin is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-gamma-catenin-primary-antibodies-ihc-testing-1.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg;IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). <br>gamma Catenin was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-2-ihc-anti-gamma-catenin-picoband-antibody.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). <br> gamma Catenin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-3-ihc-anti-gamma-catenin-picoband-antibody.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). <br> gamma Catenin was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-4-ihc-anti-gamma-catenin-picoband-antibody.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). <br> gamma Catenin was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-5-ihc-anti-gamma-catenin-picoband-antibody.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; IHC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). <br> gamma Catenin was detected in frozen section of rat intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-6_1.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; ICC analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185).<br> gamma Catenin was detected in immunocytochemical section of human MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9185-7_1.jpgAnti-gamma Catenin/JUP Antibody Picoband&reg; IF analysis of gamma Catenin using anti-gamma Catenin antibody (PB9185). <br> gamma Catenin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-gamma Catenin Antibody (PB9185) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-met-c-met-picoband-trade-antibody-pb9186-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9186-2-WB-anti-c-met-hgfr-antibody.jpgAnti-Met (c-Met) Antibody Picoband&reg; Western blot analysis of Met (c-Met) using anti-Met (c-Met) antibody (PB9186). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Met (c-Met) antigen affinity purified polyclonal antibody (Catalog # PB9186) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Met (c-Met) at approximately 154 kDa. The expected band size for Met (c-Met) is at 156 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9186-ajcr0013-4145-f3.jpgAnti-Met (c-Met) Antibody Picoband&reg;The enhanced apoptosis of the combination of luteolin and osimertinib but non-arrested the cell cycle in H1975/OR cells. The H1975/OR cells were treated with 1 μM osimertinib (OSI) alone or combined with 10-150 μM luteolin (LUT) for 24 h. Apoptosis (A) and the cell cycle (B) were detected with flow cytometry.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10560942/&orig_host=www.ncbi.nlm.nih.gov'>37818074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9186-ajcr0013-4145-f4.jpgAnti-Met (c-Met) Antibody Picoband&reg;The inhibition of HGF-MET-Akt pathway in MET-amplified osimertinib-acquired resistant H1975/OR cells by the combination of luteolin and osimertinib. A. Relative MET gene copy numbers in H1975 and H1975/OR cell lines at different luteolin and osimertinib concentrations. B. MET, p-MET, Akt 1,2,3, and p-Akt protein expression in H1975 and H1975/OR cells at different luteolin and osimertinib concentrations. C. HGF protein expression in H1975 and H1975/OR cell lines at different luteolin and osimertinib concentrations.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10560942/&orig_host=www.ncbi.nlm.nih.gov'>37818074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9186-ajcr0013-4145-f5.jpgAnti-Met (c-Met) Antibody Picoband&reg;The effects of MET silencing on the synergistic effect of luteolin and osimertinib. A. Relative MET gene copy numbers of MET siRNA with or without osimertinib (1 μM) and luteolin (50 μM) in H1975/OR cells. B. The cell proliferation inhibition effect of MET siRNA with or without osimertinib (1 μM) and luteolin (50 μM) in H1975/OR cells. C. The cell migration inhibition effect of MET siRNA with or without osimertinib (1 μM) and luteolin (10 μM) in H1975/OR cells. D and E. The 24 h and 48 h cell migration results were expressed as the increasing % of 0 h. The data are means ± SD of three experiments. ns, no significance, *, P < 0.05, **, P < 0.01. F. The cell invasion inhibition effect of MET siRNA with or without osimertinib (1 μM) and luteolin (10 μM) in H1975/OR cells. G. The cell apoptosis effect of MET siRNA with or without osimertinib (1 μM) and luteolin (50 μM) in H1975/OR cells.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10560942/&orig_host=www.ncbi.nlm.nih.gov'>37818074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9186-ajcr0013-4145-f6.jpgAnti-Met (c-Met) Antibody Picoband&reg;Stable binding of luteolin to MET by molecular docking analysis. A. The structural formula for luteolin. A, B, or C are the sequence number of the ring structure. B, C. The binding conformation of luteolin to the MET kinase domain. The colored structure shows the 3D conformation of the non-phosphorylated MET protein, and the red-green stick shows the 3D conformation of luteolin. Oxygen atoms: red stick. H-bond and length: yellow dotted line and attached number. PRO1158, TYR1159, and MET1160 active residues of MET binding to luteolin are represented by pink, purple, and grey sticks, respectively. D. Surface representation of luteolin docking in the binding pocket of MET kinase. E. The sensorgram of luteolin binding to MET-immobilized chip. The luteolin concentrations were 0.0390625, 0.078125, 0.15625, 0.3125, 0.625, 1.25, 2.50, and 5.00 μM (from bottom to top). F. The effect of luteolin treatment on MET kinase activity was measured by ADP-Glo™ kinase assay. The data are means ± SD of three experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10560942/&orig_host=www.ncbi.nlm.nih.gov'>37818074</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-3-picoband-trade-antibody-pb9188-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-ajcr0009-0872-f8.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg;Expressions of Ki-67, Caspase 3 were detected by immunohistochemical staining in the carcinoma stage (200 ×). Staining of colonic tissue for Ki-67 (Upper) and Caspase 3 (Lower) of 4-μm sections from paraffinembedded tissue of mice from AOM/DSS + Bs and AOM/DSS group (Scale bar: 50 μm). Ki-67 and Caspase 3 staining was performed per manufacturers’ instructions. Data are representative of three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556602/&orig_host=www.ncbi.nlm.nih.gov'>31218099</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-nrr-9-837-g002.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg;Expression of GFAP, caspase-3 and caspase-9 at various time points after adipose-derived stromal cells differentiation (immunocytochemical staining, inverted phase contrast microscope, × 200). Arrows indicate GFAP-, caspase-3- and caspase-9-positive cells. GFAP: Glial fibrillary acidic protein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4146249/&orig_host=www.ncbi.nlm.nih.gov'>25206897</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-nrr-9-837-g003.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg;Positive expression rate of glial fibrillary acidic protein (GFAP), caspase-3 and caspase-9 in adult adipose-derived stromal cells differentiated at different time points (immunocytochemistry). Data were expressed as mean ± SD. Differences were compared using one-way analysis of variance followed by Student-Newman-Keuls test. Experiments were repeated in triplicate. * P < 0.05, vs . previous time point.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4146249/&orig_host=www.ncbi.nlm.nih.gov'>25206897</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-djir_a_12304658_f0001_c.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg;Results of network pharmacology and molecular docking. (A) Curcumin and OA- and chondrocyte-related target distribution. Screened drugs may act on the core targets. (B) The interaction network of potential targets of curcumin in the treatment of osteoarthritis, wherein the top six targets of node degree are indicated with pink color. (C and D) BPs, CCs, MFs and KEGG analysis results, The size of the bubble represents the number of significantly different related targets enriched in the pathway, and the points with different color gradients represent the range of p values. The higher the enrichment factor value, the higher the enrichment degree, and the lower the p value, the higher the enrichment degree. (E) Curcumin was docked with Bcl-2, caspase-3, and Mmp-9, respectively. The red structure represents curcumin; the blue fragment on the protein structure represents the amino acid that forms hydrogen bonds with curcumin; and the yellow dotted line represents the hydrogen bond formed between the molecule and the protein. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/JIR.S459867'>39081871</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-fphar-16-1650534-g004.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg;Regulatory effects of KSG on the expression of cytokines and apoptosis-related factors in Aβ-induced PC12 cells. (A) Expression of IL-1β (n = 3). (B) Expression of IL-18 (n = 3). (C) Expression of TNF-α (n = 3). (D) Protein expression of apoptosis-related factors in PC12 cells of each group (n = 3). (E) Expression of Caspase-3 (n = 3). (F) Expression of Bax (n = 3). Data were expressed as mean ± SD. * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. Con. # P < 0.05, ## P < 0.01, and ### P < 0.001 vs. AD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1650534/full'>41050397</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-casp3-primary-antibodies-wb-testing-1_1.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg; Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: rat thymus tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates,<br> Lane 6: mouse RAW264.7 whole cell lysates,<br> Lane 7: mouse ANA-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 antigen affinity purified polyclonal antibody (Catalog # PB9188) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 at approximately 35 kDa, 17 kDa (Cleaved). The expected band size for Caspase-3 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-casp3-primary-antibodies-wb-testing-2_1.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg; Western blot analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3 antigen affinity purified polyclonal antibody (Catalog # PB9188) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3 at approximately 35 kDa, 17 kDa (Cleaved). The expected band size for Caspase-3 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-casp3-primary-antibodies-ihc-testing-3.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg; IHC analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188). <br> Caspase-3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caspase-3 Antibody (PB9188) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9188-casp3-primary-antibodies-if-testing-4.jpgAnti-Caspase-3/CASP3 Antibody Picoband&reg; IF analysis of Caspase-3 using anti-Caspase-3 antibody (PB9188). <br> Caspase-3 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Caspase-3 Antibody (PB9188) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp27-picoband-trade-antibody-pb9237-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9237-hsp27-primary-antibodies-wb-testing-1.jpgAnti-Hsp27/HSPB1 Antibody Picoband&reg; Western blot analysis of HSP27 using anti-HSP27 antibody (PB9237). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human CACO-2 whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates, <br> Lane 5: human HT1080 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP27 antigen affinity purified polyclonal antibody (Catalog # PB9237) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP27 at approximately 27 kDa. The expected band size for HSP27 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9237-hsp27-primary-antibodies-ihc-testing-2.jpgAnti-Hsp27/HSPB1 Antibody Picoband&reg; IHC analysis of HSP27 using anti-HSP27 antibody (PB9237). <br> HSP27 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSP27 Antibody (PB9237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9237-hsp27-primary-antibodies-ihc-testing-3.jpgAnti-Hsp27/HSPB1 Antibody Picoband&reg; IHC analysis of HSP27 using anti-HSP27 antibody (PB9237). <br> HSP27 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSP27 Antibody (PB9237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9237-hsp27-primary-antibodies-ihc-testing-4.jpgAnti-Hsp27/HSPB1 Antibody Picoband&reg; IHC analysis of HSP27 using anti-HSP27 antibody (PB9237). <br> HSP27 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSP27 Antibody (PB9237) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9237-hsp27-primary-antibodies-if-testing-5.jpgAnti-Hsp27/HSPB1 Antibody Picoband&reg; IF analysis of HSP27 using anti-HSP27 antibody (PB9237).<br> HSP27 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSP27 Antibody (PB9237) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9237-hsp27-primary-antibodies-fcm-testing-6.jpgAnti-Hsp27/HSPB1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-HSP27 antibody (PB9237). <br> Overlay histogram showing A431 cells stained with PB9237 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSP27 Antibody (PB9237&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9237-hsp27-primary-antibodies-ip-testing-7.jpgAnti-Hsp27/HSPB1 Antibody Picoband&reg; Immunoprecipitating HSP27 in Hela whole cell lysate. <br>Western blot analysis of HSP27 using anti-HSP27 antibody (PB9237). <br>Lane 1: Hela whole cell lysates (30ug), <br>Lane 2: Rabbit control IgG instead of anti-HSP27 antibody in Hela whole cell lysate, <br>Lane 3: anti-HSP27 antibody (2μg) + Hela whole cell lysate (500μg). <br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HSP27 antigen affinity purified polyclonal antibody (PB9237) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for HSP27 at approximately 27 kDa. The expected band size for HSP27 is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zeb2-picoband-trade-antibody-pb9236-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9236-zeb2-primary-antibodies-wb-testing-1.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; Western blot analysis of ZEB2 using anti-ZEB2 antibody (PB9236). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZEB2 antigen affinity purified polyclonal antibody (Catalog # PB9236) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZEB2 at approximately 200 kDa. The expected band size for ZEB2 is at 200 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9236-3.pngAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-ZEB2 antibody (PB9236). <br> Overlay histogram showing U937 cells stained with PB9236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9236-4.pngAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-ZEB2 antibody (PB9236). <br> Overlay histogram showing THP-1 cells stained with PB9236 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZEB2 Antibody (PB9236&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9236-zeb2-primary-antibodies-ihc-testing-5.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; IHC analysis of ZEB2 using anti-ZEB2 antibody (PB9236). <br> ZEB2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZEB2 Antibody (PB9236) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9236-zeb2-primary-antibodies-ihc-testing-6.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; IHC analysis of ZEB2 using anti-ZEB2 antibody (PB9236). <br> ZEB2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZEB2 Antibody (PB9236) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9236-zeb2-primary-antibodies-ihc-testing-7.jpgAnti-Smad Interacting Protein 1/ZEB2 Antibody Picoband&reg; IHC analysis of ZEB2 using anti-ZEB2 antibody (PB9236). <br> ZEB2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZEB2 Antibody (PB9236) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p73-picoband-trade-antibody-pb9235-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9235-1_1-WB-anti-p73-picoband-antibody.jpgAnti-p73/TP73 Antibody Picoband&reg;Anti-p73 antibody&#44; PB9235&#44; Western blotting<br>All lanes: Anti p73 (PB9235) at 0.5ug/ml<br>WB: Recombinant Human p73 Protein 0.5ng<br>Predicted bind size: 45KD<br> Observed bind size: 45KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9235-2-WB-anti-p73-picoband-antibody.jpgAnti-p73/TP73 Antibody Picoband&reg;Anti-p73 antibody&#44; PB9235&#44; Western blotting<br>All lanes: Anti p73 (PB9235) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Lane 3: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 73KD<br>Observed bind size: 73KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9235-3-IHC-anti-p73-picoband-antibody.jpgAnti-p73/TP73 Antibody Picoband&reg;Anti-p73 antibody&#44; PB9235&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9235-4-IHC-anti-p73-picoband-antibody.jpgAnti-p73/TP73 Antibody Picoband&reg;Anti-p73 antibody&#44; PB9235&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9235-5-IHC-anti-p73-picoband-antibody.jpgAnti-p73/TP73 Antibody Picoband&reg;Anti-p73 antibody&#44; PB9235&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tjp1-picoband-trade-antibody-pb9234-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-tjp1-primary-antibodies-wb-testing-1.jpgAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg; Western blot analysis of TJP1 using anti-TJP1 antibody (PB9234). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates, <br> Lane 2: human CACO-2 whole cell lysates, <br> Lane 3: human COLO320 whole cell lysates, <br> Lane 4: rat testis tissue lysates, <br> Lane 5: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP1 antigen affinity purified polyclonal antibody (Catalog # PB9234) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP1 at approximately 220 kDa. The expected band size for TJP1 is at 185 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-12951_2025_3450_fig2_html.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;GNS protected against RA-induced joint damage and bone erosion in CIA mice. A Arthritis scores in CIA mice. * indicated significant differences between RA + GNS and RA + NS, # indicated significant differences between RA + GNS + CH and RA + GNS, + indicated differences between RA + ABX and RA + NS, and ▲indicated differences between FMT(RA + GNS) and RA + NS. B Representative histology images of joints. C Histological scores of joints. D Representative TRAP staining images of joints. E Quantitative analyses of TRAP staining. F Representative photographs and micro-CT images of paws. The bright areas in the micro-CT images of the metacarpophalangeal joints on the left were the delineated region of interest (ROI) used for subsequent bone histomorphometry index analysis. G Quantitative analyses of bone histomorphometry indexes including bone mineral density (BMD), bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in CIA mice. H Representative immunohistochemical images showing in situ expression of Claudin-1 and ZO-1. I and J The Average Optical Density (AOD) of Claudin-1 and ZO-1 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-025-03450-7'>40414887</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-12865_2025_700_fig2_html.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;The results of mouse intestinal mucosa HE staining and tight junction proteins ZO-1 and Occludin immunohistochemical staining ( n = 3). A : NC group HE staining; B : Xylitol Control group HE staining results; C : DSS group HE staining results; D : Xylitol group HE staining results; E : NC group ZO-1 immunohistochemical staining; F : Xylitol Control ZO-1 immunohistochemical staining; G : DSS group ZO-1 immunohistochemical staining; H : Xylitol group ZO-1 immunohistochemical staining; I : NC group Occludin immunohistochemical staining; J : Xylitol Control group Occludin immunohistochemical staining; K : DSS group Occludin immunohistochemical staining; L : Xylitol group Occludin immunohistochemical staining; M : ZO-1 protein expression level; N : Occludin protein expression level <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12865-025-00700-z'>40065221</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-41598_2025_85148_fig3_html.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;The effects of RSV on intestinal tight junction proteins expression. ( A ) Claudin-2, ( B ) Occludin, ( C ) ZO-1 was measured by immunofluorescence (400×). ( D-F ) IPP6.0 software was used to analyze the optical density of immunofluorescence photos. We used Gel-Pro analyzer software to quantify. Data were presented as mean ± SD, n = 5 rats per group. Compared with the control group, * P < 0.01, compared with the model group, ** P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-85148-2'>39755761</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-41598_2024_72793_fig2_html.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;Effects of LF on jejunal tissue and intestinal mucosal barrier in depressed rats (a) jejunal tissues stained with HE (b) ZO-1 expression in jejunal tissue from three groups of rats (c) The relative expression of ZO-1 is indicated by the mean optical density (MOD). Note: Compared to group K, *p < 0.05, **p < 0.01; and compared to group M, #p < 0.05, ##p < 0.01. K control group; M model group; L LF treatment group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-72793-2'>39333605</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-41598_2024_65061_fig5_html.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;Effect of Lacticaseibacillus rhamnosus E9 administration on tight junction proteins and SOX-10 in ileum of MPTP-induced model of PD compared to the control. ( A ) Immunohistochemical staining of ileal ZO-1 (100 ×), Occludin (100 ×) (staining intensity is evaluated in ideal mucosa), and SOX-10 (200 ×, inset-4000 ×) (Arrows are nuclear positivity in myenteric plexus). ( B ) Protein expression of ileal ZO-1, Occludin, and SOX-10 in PD and control mice. ( C ) Gene expression of ileal ZO-1 and Occludin in PD and control mice. MPTP mice were received (i.p.) 30 mg/kg MPTP-HCl daily for 5 consecutive days (MPTP and MPTP + P). Probiotic mice were administered 1 dose (10 8 CFU/mouse/day) daily of L. rhamnosus E9 for fifteen days and sacrificed after the last dose (P and MPTP + P). *p ≤ 0.05, control vs MPTP (n:4–5/group). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-65061-w'>38965287</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-12974_2018_1342_fig6_html.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;Tight junction formation by HBMVEC under flow conditions as indicated by immunofluorescence staining of ZO-1. PKCδ inhibition (PKCδ- i ) attenuates TNF-α-induced tight junction damage in vitro in B 3 C. When cultured with normal media, tight junctions were fully established between adjacent cells ( a ). Tight junction expression was disrupted after 4 h of TNF-α activation ( b ), while PKCδ inhibition (TNF-α + PKCδ- i ) restored tight junction expression ( c ). HBMVEC cultured for 72 h under flow (0.1 μl/min) were stained with ZO-1 (red) and Hoechst 33342 (blue). d Quantitative analysis to the total tight junction fluorescence intensity confirmed our observation. Data are presented as mean ± SEM ( n = 3). *** p < 0.001 compared to no treatment and TNF-α + PKCδ- i by ANOVA with Tukey-Kramer post hoc <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-018-1342-y'>30400800</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-fmicb-11-622354-g004.jpgAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;The subcellular localization and relative expression level detection of ZO-1 and villin in the intestinal mucosa of IBD at 7 days after termination of DSS intake. (A) The ZO-1 immunohistochemistry staining of the small intestinal epithelial TJP (brown dots): (A1) the normal group: the villi and crypts were arranged compactly, and the ZO-1-positive staining (representing the TJP) showed the dotted line (brown) along the surface of the villi and the crypts; (A2) the DSS group: ZO-1 distributed dispersively in the residual villi of the small intestinal mucosa; (A3) the DSS + B. subtilis -fermented milk group: the ZO-1 staining formed the dotted line (brown, representing the TJP) at the subsurface of the regenerative villi. (B) The ZO-1 immunohistochemistry staining of the colonic epithelial TJP (brown dots): (B1) the normal group: ZO-1-positive staining distributed on the inner side of the epithelial cell membrane (representing the TJP); (B2) the DSS group: there was no ZO-1-positive staining in the scar; (B3) the DSS + B. subtilis -fermented milk group: the ZO-1-positive staining distributed on the inner side of the regenerative epithelial cell membrane (representing the TJP). (C) The villin immunohistochemistry staining (brown strip) of the small intestinal microvilli: (C1) the normal group: villin-positive staining showed a strip-like distribution on the surface of the villi in the normal small intestinal mucosa; (C2) the DSS group: villin distributed at the surface of the residual villi; (C3) the DSS + B. subtilis -fermented milk group: villin-positive staining formed an integrative strip (brown) enclosing the surface of the regenerative villi. (D) The villin immunohistochemistry staining of the colonic epithelium: (D1) the normal group: villin-positive staining (brown) showed banded distribution on the surface of the epithelium; (D2) the DSS group: almost no villin-positive staining was observed in the scar due to damage of the epithelium; (D3) the DSS + B. subtilis -fermented milk group: the villin-positive staining (brown) showed banded distribution on the surface of the regenerated epithelium in the colonic mucosa. (E,F) The western blotting analysis for the relative expression level of ZO-1 and villin in the samples contained equivalent ileum and colon. The expression level of ZO-1 and villin in the DSS group was significantly lower than that of the normal (control) group. The expression level of ZO-1 and villin and in the DSS + B. subtilis -fermented milk (FM) group was significantly higher than that of the DSS group ( n = 5, * represents p < 0.05, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-oncotarget-08-91291-g005.jpgAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;Simvastatin reversed protein expression of high glucose-induced ZO-1 and VE-Cadherin. RAECs were incubated with high glucose for 24h, which was treated with simvastatin (SIM, 5μM) in the presence or absence of NLRP3 siRNA or MCC950 (15nM). Representative Western blot gel documents (A, B) and summarized data showing the protein expression of ZO-1 (C, D) and VE-Cadherin (E, F) . (G) Representative fluorescence images showing the cell membrane fluorescence of ZO-1 from at least three independent experiments. (H) Frequency histogram of ZO-1 in the membranes showing the protein expression of ZO-1 by flowcytometry. * P <0.05 vs. Scram Vehl or Ctrl Vehl; # P <0.05 vs. HG treated group (n=4).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5710924/&orig_host=www.ncbi.nlm.nih.gov'>29207644</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-fft270164-fig-0008-m.jpgAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;Effects of MT water extract on regulating macrophage polarization, maintaining intestinal barrier integrity, and alleviating oxidative stress in the colons of DSS-induced UC mice. (A) IHC staining of iNOS, Arg1, occludin, and ZO-1 expressions in mouse colon tissues. Quantitative analysis of the integrated optical density (IOD) of (B) iNOS, (C) Arg1, (D) occludin, and (E) ZO-1 in each group. The levels of (F) IL-6, (G) IL-10, (H) MDA, and (I) GSH were measured and analyzed. Data were presented as means ± SD for three independent trials. One-way ANOVA followed by Tukey's multiple comparison test was performed to compare the differences between groups. *p < 0.05 versus control and #p < 0.05 versus DSS alone group. Arg1, arginase 1; DSS, dextran sulfate sodium; IL, interleukin; iNOS, inducible nitric oxide synthase; MDA, malondialdehyde; MT, Medulla Tetrapanacis.<br><b>Index in Food Frontiers under a CC BY license. DOI: <a href='0'>10.1002/fft2.70164</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9234-3-IHC-anti-tjp1-tight-junction-protein-1-picoband-antibody.jpgAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg; IHC analysis of TJP1 using anti-TJP1 antibody (PB9234).<br>TJP1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TJP1 Antibody (PB9234) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-3_1.jpgAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg; IF analysis of TJP1 using anti-TJP1 antibody (PB9234). <br> TJP1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TJP1 Antibody (PB9234) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-4.jpgAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-TJP1 antibody (PB9234).<br>Overlay histogram showing K562 cells stained with PB9234 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TJP1 Antibody (PB9234,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-tjp1-primary-antibodies-wb-testing-1.jpg.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;IF analysis of TJP1 using anti-TJP1 antibody (PB9234). <br> TJP1 was detected in an immunocytochemical section of Endothelial cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1:200 rabbit anti-TJP1 Antibody (PB9234) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9234-tjp1-primary-antibodies-icc-testing-3.pngAnti-ZO1 tight junction protein/TJP1 Antibody Picoband&reg;IF analysis of TJP1 using anti-TJP1 antibody (PB9234). <br> TJP1 was detected in an immunocytochemical section of human Caco-2 cells. Cells were normally cultured in 24-well plates using MEM supplemented with 20% fetal bovine serum. When the cell density reached approximately 60%, the culture was stopped. The medium was removed, cells were washed three times with PBS, fixed with 4% paraformaldehyde for 15 minutes, and then washed three times with PBS before further use. The cells were blocked with 10% goat serum. And then incubated with 1:200 rabbit anti-TJP1 Antibody (PB9234) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 45 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c5a-picoband-trade-antibody-pb9187-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9187-1_1-WB-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg; Western blot analysis of C5A using anti-C5A antibody (PB9187). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant rat C5A protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5A antigen affinity purified polyclonal antibody (Catalog # PB9187) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5A at approximately 37 kDa. The expected band size for C5A is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9187-2_1-WB-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg; Western blot analysis of C5A using anti-C5A antibody (PB9187). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C5A antigen affinity purified polyclonal antibody (Catalog # PB9187) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C5A at approximately 115 kDa. The expected band size for C5A is at 115 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9187-3_1-IHC-anti-complement-c5a-antibody.jpgAnti-C5/C5a Antibody Picoband&reg; IHC analysis of C5A using anti-C5A antibody (PB9187). <br> C5A was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTAbuffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-C5A Antibody (PB9187) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tfrc-picoband-trade-antibody-pb9233-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-tfrc-primary-antibodies-wb-testing-1.jpgAnti-Transferrin Receptor/TFRC Antibody Picoband&reg; Western blot analysis of TFRC using anti-TFRC antibody (PB9233). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human HT1080 whole cell lysates, <br> Lane 4: rat thymus tissue lysates, <br> Lane 5: rat RAW264.7 whole cell lysates, <br> Lane 6: mouse SP2/0 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFRC antigen affinity purified polyclonal antibody (Catalog # PB9233) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFRC at approximately 95 kDa. The expected band size for TFRC is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-2-ihc-anti-tfrc-transferrin-receptor-picoband-antibody.jpgAnti-Transferrin Receptor/TFRC Antibody Picoband&reg; IHC analysis of TFRC using anti-TFRC antibody (PB9233). <br> TFRC was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TFRC Antibody (PB9233) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-tfrc-primary-antibodies-wb-testing-2.jpg.pngAnti-Transferrin Receptor/TFRC Antibody Picoband&reg;Western blot analysis of TFRC using anti-TFRC antibody (PB9233). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1-3: model group-Mouse uterine tissue, <br> Lane 4-6: young group-Mouse uterine tissue.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFRC antigen affinity purified polyclonal antibody (PB9233) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TFRC at approximately 85 kDa. The expected band size for TFRC is at 85 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-3_1.jpgAnti-Transferrin Receptor/TFRC Antibody Picoband&reg; IHC analysis of TFRC using anti-TFRC antibody (PB9233). <br> TFRC was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TFRC Antibody (PB9233) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-4_1.jpgAnti-Transferrin Receptor/TFRC Antibody Picoband&reg; IF analysis of TFRC using anti-TFRC antibody (PB9233).<br> TFRC was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TFRC Antibody (PB9233) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-tfrc-primary-antibodies-if-testing-5.jpgAnti-Transferrin Receptor/TFRC Antibody Picoband&reg; IF analysis of TFRC using anti-TFRC antibody (PB9233). <br> TFRC was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-TFRC Antibody (PB9233) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-6.pngAnti-Transferrin Receptor/TFRC Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-TFRC antibody (PB9233). <br> Overlay histogram showing SiHa cells stained with PB9233 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFRC Antibody (PB9233&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9233-7.pngAnti-Transferrin Receptor/TFRC Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-TFRC antibody (PB9233). <br> Overlay histogram showing U87 cells stained with PB9233 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFRC Antibody (PB9233&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp2e1-picoband-trade-antibody-pb9190-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9190-1.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband&reg; Western blot analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>lane 1: rat liver tissue lysate&#44;<br>lane 2: mouse liver tissue lysate.<br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody (Catalog # PB9190) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 55KD. The expected band size for CYP2E1 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9190-cyp2e1-primary-antibodies-ihc-testing-4.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband&reg;IHC analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190). <br>CYP2E1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP2E1 Antibody (PB9190) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9190-3-IHC-anti-cyp2e1-picoband-antibody.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband&reg; IHC analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190).<br> CYP2E1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (PB9190) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9190-4-IHC-anti-cyp2e1-picoband-antibody.jpgAnti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband&reg; IHC analysis of CYP2E1 using anti-CYP2E1 antibody (PB9190).<br> CYP2E1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP2E1 Antibody (PB9190) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nr3c1-picoband-trade-antibody-pb9232-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9232-gr-primary-antibodies-fcm-testing-6_1.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-NR3C1 antibody (PB9232). <br>Overlay histogram showing SiHa cells stained with PB9232 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NR3C1 Antibody (PB9232, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9232-gr-primary-antibodies-wb-testing-1_1.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; Western blot analysis of NR3C1 using anti-NR3C1 antibody (PB9232). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human HEK293 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C1 antigen affinity purified polyclonal antibody (Catalog # PB9232) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR3C1 at approximately 95 kDa. The expected band size for NR3C1 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9232-gr-primary-antibodies-ihc-testing-2_1.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9232). <br> NR3C1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NR3C1 Antibody (PB9232) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9232-gr-primary-antibodies-ihc-testing-3_1.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9232). <br> NR3C1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NR3C1 Antibody (PB9232) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9232-gr-primary-antibodies-ihc-testing-4_1.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9232). <br> NR3C1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NR3C1 Antibody (PB9232) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9232-gr-primary-antibodies-if-testing-5_1.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; IF analysis of NR3C1 using anti- NR3C1 antibody (PB9232). <br> NR3C1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti- NR3C1 Antibody (PB9232) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eda-picoband-trade-antibody-pb9191-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9191-1-WB-anti-eda-eda-a2-picoband-antibody.jpgAnti-EDA Antibody Picoband&reg; Western blot analysis of EDA using anti-EDA antibody (PB9191). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Colo320 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDA antigen affinity purified polyclonal antibody (Catalog # PB9191) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDA at approximately 41 kDa. The expected band size for EDA is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9191-2-WB-anti-eda-eda-a2-picoband-antibody.jpgAnti-EDA Antibody Picoband&reg; Western blot analysis of EDA using anti-EDA antibody (PB9191). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: recombinant human EDA protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDA antigen affinity purified polyclonal antibody (Catalog # PB9191) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDA at approximately 43 kDa. The expected band size for EDA is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-estrogen-receptor-picoband-trade-antibody-pb9192-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9192-esr1-primary-antibodies-wb-testing-1.jpgAnti-Estrogen Receptor/ESR1 Antibody Picoband&reg; Western blot analysis of Estrogen Receptor using anti-Estrogen Receptor antibody (PB9192). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human T-47D whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Estrogen Receptor antigen affinity purified polyclonal antibody (Catalog # PB9192) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Estrogen Receptor at approximately 66 kDa. The expected band size for Estrogen Receptor is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9192-oncotarget-09-4475-g001.jpgAnti-Estrogen Receptor/ESR1 Antibody Picoband&reg;Immunohistochemical stain of HEV ORF2 and ORF3 antigen. ( A – C ) Expression of HEV ORF2 in ovarian tissues, no positive signals in ovaries from control group (A). The positive signals distribute in the sperm cell and ovum cytoplasm of HEV-positive ovarian section at 28 dpi (B) and 49 dpi (C). ( D – E ) Expression of HEV ORF3 in ovarian tissues, no positive signals in ovaries from control group (D). The positive signals distribute in the sperm cell and ovum cytoplasm of HEV-positive ovarian section at 28 dpi (E) and 49 dpi ( F ). (Magnification: 20×).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5796988/&orig_host=www.ncbi.nlm.nih.gov'>29435117</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9192-oncotarget-09-4475-g002.jpgAnti-Estrogen Receptor/ESR1 Antibody Picoband&reg;Histopathological analyze of ovaries. ( A ) and ( D ), There were no gross histopathological lesions in the ovarian section from the control group at 28 dpi and 49 dpi, respectively.(Magnification: 10×); ( B ) ovarian interstitial cell scattered necrosis (black arrow), a small amount of lymphocytic infiltration in part area of ovarian interstitial from HEV RNA positive rabbit at 28 dpi.(Magnification:10×); ( C ) Necrosis and scattered lymphocytic infiltration (black arrow) in part area of ovarian interstitial from HEV RNA positive rabbit at 28 dpi.(Magnification:40×); ( D ) high power field of the control group (Magnification:20×); ( E ) Large amounts of ovarian germ cell were necrosis (black arrow) and dropped off, an increase of ovarian atresia and lymphpcytic infiltration in the ovarian interstitial from the HEV RNA positive rabbit at 48 dpi. (Magnification:10×); ( F ) Large amounts of lymphcytic infiltration (green arrow) in the ovarian interstitial from the HEV RNA positive rabbit. (Magnification:40×).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5796988/&orig_host=www.ncbi.nlm.nih.gov'>29435117</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9192-oncotarget-09-4475-g004.jpgAnti-Estrogen Receptor/ESR1 Antibody Picoband&reg;Representative TUNEL-stained histological sections of ovaries from control group and Swine HEV inoculated rabbits. TUNEL-positive cells have brown nuclei. The number of TUNEL-positive cells were remarkably higher in HEV RNA positive ovaries at 28dpi ( B ) and 49dpi ( C ) than in the control ( A ). (magnification:40×). Quantitative analysis of TUNEL-positive cells in ovaries of rabbits ( D ). The data were expressed as the percentage (mean ± SD). ( * P < 0.05) or ( ** P < 0.01), indicated statistical significance versus the control group ( n = 5).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5796988/&orig_host=www.ncbi.nlm.nih.gov'>29435117</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9192-oncotarget-09-4475-g006.jpgAnti-Estrogen Receptor/ESR1 Antibody Picoband&reg;Expression level of protein of Bcl-2, Bax and Caspase-3 in ovary tissue of different groups at 28 dpi and 49 dpi. ( A ) intensity of expression of Bcl-2 ( B ) Bax, ( C ) Caspase-3 in ovaries of HEV inoculation rabbits. ( D ) Western blot analysis of Bcl-2, Bax and Caspase-3 in ovaries in different groups.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5796988/&orig_host=www.ncbi.nlm.nih.gov'>29435117</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9192-2-ihc-anti-estrogen-receptor-picoband-antibody.jpgAnti-Estrogen Receptor/ESR1 Antibody Picoband&reg; IHC analysis of Estrogen Receptor using anti-Estrogen Receptor antibody (PB9192). <br> Estrogen Receptor was detected in a paraffin-embedded section of human mammry cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Estrogen Receptor Antibody (PB9192) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-klf6-picoband-trade-antibody-pb9231-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9231-1-WB-anti-klf6-picoband-antibody.jpgAnti-KLF6 Antibody Picoband&reg;Anti-KLF6 antibody&#44; PB9231&#44; Western blotting<br>All lanes: Anti KLF6 (PB9231) at 0.5ug/ml<br>WB: Recombinant Human KLF6 Protein 0.5ng<br>Predicted bind size: 36KD<br>Observed bind size: 36KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9231-2-WB-anti-klf6-picoband-antibody.jpgAnti-KLF6 Antibody Picoband&reg;Anti-KLF6 antibody&#44; PB9231&#44; Western blotting<br>All lanes: Anti KLF6 (PB9231) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: Rat Testis Tissue Lysate at 50ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Lane 4: HEPG2 Whole Cell Lysate at 40ug<br>Lane 5: HEPA Whole Cell Lysate at 40ug<br>Predicted bind size: 32KD<br>Observed bind size: 37KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgfr4-picoband-trade-antibody-pb9193-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9193-1-WB-anti-fgfr4-picoband-antibody.jpgAnti-FGFR4 Antibody Picoband&reg; Western blot analysis of FGFR4 using anti-FGFR4 antibody (PB9193). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human FGFR4 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified polyclonal antibody (Catalog # PB9193) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 39 kDa. The expected band size for FGFR4 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9193-2-WB-anti-fgfr4-picoband-antibody.jpgAnti-FGFR4 Antibody Picoband&reg; Western blot analysis of FGFR4 using anti-FGFR4 antibody (PB9193). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human PANC whole cell lysates, <br> Lane 3: human SGC whole cell lysates, <br> Lane 4: human COLO320 whole cell lysates, <br> Lane 5: human SW620 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified polyclonal antibody (Catalog # PB9193) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 100 kDa. The expected band size for FGFR4 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9193-3-IHC-anti-fgfr4-picoband-antibody.jpgAnti-FGFR4 Antibody Picoband&reg; IHC analysis of FGFR4 using anti-FGFR4 antibody (PB9193). <br> FGFR4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FGFR4 Antibody (PB9193) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-foxm1-picoband-trade-antibody-pb9194-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9194-1-WB-anti-foxm1-picoband-antibody.jpgAnti-FOXM1 Antibody Picoband&reg; Western blot analysis of FOXM1 using anti-FOXM1 antibody (PB9194). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human COLO320 whole cell lysates, <br> Lane 3: human SW620 whole cell lysates, <br> Lane 4: human SKOV whole cell lysates, <br> Lane 5: human MCD-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXM1 antigen affinity purified polyclonal antibody (Catalog # PB9194) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXM1 at approximately 84 kDa. The expected band size for FOXM1 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9194-2-WB-anti-foxm1-picoband-antibody.jpgAnti-FOXM1 Antibody Picoband&reg; Western blot analysis of FOXM1 using anti-FOXM1 antibody (PB9194). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human FOXM1 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXM1 antigen affinity purified polyclonal antibody (Catalog # PB9194) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXM1 at approximately 36 kDa. The expected band size for FOXM1 is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcak-picoband-trade-antibody-pb9230-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9230-mcak-primary-antibodies-wb-testing-1.jpgAnti-MCAK/KIF2C Antibody Picoband&reg; Western blot analysis of MCAK using anti-MCAK antibody (PB9230). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCAK antigen affinity purified polyclonal antibody (Catalog # PB9230) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCAK at approximately 81 kDa. The expected band size for MCAK is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9230-kif2c-primary-antibodies-ihc-testing-6.jpgAnti-MCAK/KIF2C Antibody Picoband&reg;IHC analysis of KIF2C using anti-KIF2C antibody (PB9230). <br>KIF2C was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KIF2C Antibody (PB9230) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9230-5-IHC-anti-mcak-picoband-antibody.jpgAnti-MCAK/KIF2C Antibody Picoband&reg;Anti-MCAK antibody&#44; PB9230&#44; IHC(P)<br>IHC(P): Rat Thymus Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9230-4-IHC-anti-mcak-picoband-antibody.jpgAnti-MCAK/KIF2C Antibody Picoband&reg;Anti-MCAK antibody&#44; PB9230&#44; IHC(P)<br>IHC(P): Rat Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9230-3-IHC-anti-mcak-picoband-antibody.jpgAnti-MCAK/KIF2C Antibody Picoband&reg;Anti-MCAK antibody&#44; PB9230&#44; IHC(P)<br>IHC(P): Mouse Testis Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9230-mcak-primary-antibodies-if-testing-6.jpgAnti-MCAK/KIF2C Antibody Picoband&reg; IF analysis of MCAK using anti-MCAK antibody (PB9230). <br> MCAK was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCAK Antibody (PB9230) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9230-mcak-primary-antibodies-fcm-testing-7.jpgAnti-MCAK/KIF2C Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-MCAK antibody (PB9230).<br>Overlay histogram showing K562 cells stained with PB9230 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCAK Antibody (PB9230,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-foxo1a-picoband-trade-antibody-pb9195-boster.html2026-03-24T05:04:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9195-1-WB-anti-foxo1a-picoband-antibody.jpgAnti-FOXO1A Antibody Picoband&reg; Western blot analysis of FOXO1A using anti-FOXO1A antibody (PB9195). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human FOXO1A protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO1A antigen affinity purified polyclonal antibody (Catalog # PB9195) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO1A at approximately 39 kDa. The expected band size for FOXO1A is at 39 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9195-2-WB-anti-foxo1a-picoband-antibody.jpgAnti-FOXO1A Antibody Picoband&reg; Western blot analysis of FOXO1A using anti-FOXO1A antibody (PB9195). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: human COLO320 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO1A antigen affinity purified polyclonal antibody (Catalog # PB9195) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO1A at approximately 70 kDa. The expected band size for FOXO1A is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kv4-3-picoband-trade-antibody-pb9229-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9229-2-WB-anti-kv4-3-picoband-antibody.jpgAnti-Kv4.3/KCND3 Antibody Picoband&reg;Anti-Kv4.3 antibody&#44; PB9229&#44; Western blotting<br>All lanes: Anti Kv4.3 (PB9229) at 0.5ug/ml<br>WB: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 73KD<br>Observed bind size: 73KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-foxo3a-picoband-trade-antibody-pb9196-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-fgene-13-922807-g004.jpgAnti-FOXO3A Antibody Picoband&reg;Arbutin exerted protective effects via the SIRT1/FOXO3A/PGC-1α/β and NF-κB/p65 signaling pathway. (A) qRT-PCR was used to measure transcript levels of the SIRT1/FOXO3a/PGC-1α/β pathway and NF-κB/p65 genes. TBHP decreased the expression of SIRT1, FOXO3a, and PGC-1α/β and increased the expression of NF-κB/p65, whereas mRNA levels in the groups that were pretreated with Arbutin showed reversed trend. (B) western blots were conducted to detect the proteins level of SIRT1, FOXO3a, PGC-1α/β, p-ERK, and NFKB1/P65. (C) imageJ was used to analyze the relative expression level of the proteins mentioned above (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 3, bars represent SD).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.922807/full'>36051689</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-fgene-13-922807-g005.jpgAnti-FOXO3A Antibody Picoband&reg;Sirtinol diminished the capability of Arbutin to assist ARPE-19 cells to defend against oxidative stress. (A) (B) flow cytometric analysis showed that cells treated with only sirtinol, TBHP, cotreated with sirtinol, and TBHP displayed decreased cellular viability. However, sirtinol conduction diminished the protective capacity of Arbutin. (C) ARPE-19 cells were seeded in a 24-well plate and applied wounds at the confluence of 80%. The cells were pretreated with or without Arbutin and then subjected to TBHP (350 µM); meanwhile, cells in certain groups were incubated with sirtinol. Photos were taken at different time points post distinct treatments. (D) fluorescence images observed that ARPE-19 treated with Arbutin while subjected to sirtinol and then exposed to TBHP was unable to recuperate ΔΨm. (E) with sirtinol administration, the protein levels of the SIRT1/FOXO3a/PGC-1α/β pathway decreased in the presence of Arbutin (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 3, bars represent SD).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2022.922807/full'>36051689</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-foxo3a-primary-antibodies-wb-testing-1.jpgAnti-FOXO3A Antibody Picoband&reg; Western blot analysis of FOXO3A using anti-FOXO3A antibody (PB9196). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: rat ovary tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO3A antigen affinity purified polyclonal antibody (Catalog # PB9196) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO3A at approximately 80-90 kDa. The expected band size for FOXO3A is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-foxo3a-primary-antibodies-ihc-testing-2.jpgAnti-FOXO3A Antibody Picoband&reg; IHC analysis of FOXO3A using anti-FOXO3A antibody (PB9196). <br> FOXO3A was detected in a paraffin-embedded section of human pancreatic ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXO3A Antibody (PB9196) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-foxo3a-primary-antibodies-ihc-testing-3.jpgAnti-FOXO3A Antibody Picoband&reg; IHC analysis of FOXO3A using anti-FOXO3A antibody (PB9196). <br> FOXO3A was detected in a paraffin-embedded section of human pancreatic ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXO3A Antibody (PB9196) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-foxo3a-primary-antibodies-ihc-testing-4.jpgAnti-FOXO3A Antibody Picoband&reg; IHC analysis of FOXO3A using anti-FOXO3A antibody (PB9196). <br> FOXO3A was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXO3A Antibody (PB9196) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-foxo3a-primary-antibodies-if-testing-5.jpgAnti-FOXO3A Antibody Picoband&reg; IF analysis of FOXO3A using anti-FOXO3A antibody (PB9196) and anti-Beta Tubulin antibody (M01857-3).<br> FOXO3A was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FOXO3A Antibody (PB9196) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9196-foxo3a-primary-antibodies-fcm-testing-6.jpgAnti-FOXO3A Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-FOXO3A antibody (PB9196). <br> Overlay histogram showing MCF-7 cells stained with PB9196 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXO3A Antibody (PB9196, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kcnma1-picoband-trade-antibody-pb9227-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9227-1-wb-anti-kcnma1-picoband-antibody.jpg.jpgAnti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband&reg; Western blot analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates,<br> Lane 2: human U87 whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNMA1 antigen affinity purified polyclonal antibody (Catalog # PB9227) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNMA1 at approximately 110-138 kDa. The expected band size for KCNMA1 is at 138KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9227-3-IHC-anti-kcnma1-picoband-antibody.jpgAnti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband&reg; IHC analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). KCNMA1 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KCNMA1 Antibody (PB9227) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9227-4-IHC-anti-kcnma1-picoband-antibody.jpgAnti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband&reg; IHC analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). KCNMA1 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KCNMA1 Antibody (PB9227) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9227-5-IHC-anti-kcnma1-picoband-antibody.jpgAnti-Maxi Potassium channel alpha/KCNMA1 Antibody Picoband&reg; IHC analysis of KCNMA1 using anti-KCNMA1 antibody (PB9227). KCNMA1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KCNMA1 Antibody (PB9227) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ip3-receptor-picoband-trade-antibody-pb9225-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-itpr1-primary-antibodies-ihc-testing-6.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg;IHC analysis of ITPR1 using anti-ITPR1 antibody (PB9225). <br>ITPR1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ITPR1 Antibody (PB9225) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-itpr1-primary-antibodies-wb-testing-1.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg; Western blot analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP3 receptor antigen affinity purified polyclonal antibody (Catalog # PB9225) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP3 receptor at approximately 290 kDa. The expected band size for IP3 receptor is at 314 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-itpr1-primary-antibodies-ihc-testing-5.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg; IHC analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225). <br> IP3 receptor was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-IP3 receptor Antibody (PB9225) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-6.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg; IF analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225). <br> IP3 receptor was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IP3 receptor Antibody (PB9225) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-7.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-IP3 receptor antibody (PB9225).<br> Overlay histogram showing U87 cells stained with PB9225 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IP3 receptor Antibody (PB9225,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-2_1-ihc-anti-ip3-receptor-picoband-antibody.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg; IHC analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225). <br> IP3 receptor was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IP3 receptor Antibody (PB9225) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-3_1-ihc-anti-ip3-receptor-picoband-antibody.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg; IHC analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225). <br> IP3 receptor was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IP3 receptor Antibody (PB9225) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9225-4_1-ihc-anti-ip3-receptor-picoband-antibody.jpgAnti-IP3 receptor/ITPR1 Antibody Picoband&reg; IHC analysis of IP3 receptor using anti-IP3 receptor antibody (PB9225).<br> IP3 receptor was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IP3 receptor Antibody (PB9225) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-g6pd-picoband-trade-antibody-pb9198-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9198-g6pd-primary-antibodies-wb-testing-1.jpgAnti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband&reg; Western blot analysis of G6PD using anti-G6PD antibody (PB9198). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human U20S whole cell lysates,<br> Lane 5: human U251 whole cell lysates,<br> Lane 6: human A431 whole cell lysates,<br> Lane 7: human HepG2 whole cell lysates,<br> Lane 8: human RT4 whole cell lysates,<br> Lane 9: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD antigen affinity purified polyclonal antibody (Catalog # PB9198) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G6PD at approximately 59 kDa. The expected band size for G6PD is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gata3-picoband-trade-antibody-pb9199-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9199-1-WB-anti-gata3-picoband-antibody.jpgAnti-GATA3 Antibody Picoband&reg; Western blot analysis of GATA3 using anti-GATA3 antibody (PB9199). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human GATA3 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 39 kDa. The expected band size for GATA3 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9199-2-WB-anti-gata3-picoband-antibody.jpgAnti-GATA3 Antibody Picoband&reg; Western blot analysis of GATA3 using anti-GATA3 antibody (PB9199). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA3 antigen affinity purified polyclonal antibody (Catalog # PB9199) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA3 at approximately 48 kDa. The expected band size for GATA3 is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irs1-picoband-trade-antibody-pb9223-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9223-1_1-WB-anti-irs1-picoband-antibody.jpgAnti-IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PB9223&#44; Western blotting<br>All lanes: Anti IRS1 (PB9223) at 0.5ug/ml<br>WB: Recombinant Human IRS1 Protein 0.5ng<br>Predicted bind size: 39KD<br>Observed bind size: 39KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9223-2_1-WB-anti-irs1-picoband-antibody.jpgAnti-IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PB9223&#44; Western blotting<br>All lanes: Anti IRS1 (PB9223) at 0.5ug/ml<br>Lane 1: A549 Whole Cell Lysate at 40ug<br>Lane 2: MM453 Whole Cell Lysate at 40ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 132KD<br>Observed bind size: 132KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9223-3_1-IHC-anti-irs1-picoband-antibody.jpgAnti-IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PB9223&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9223-4_1-IHC-anti-irs1-picoband-antibody.jpgAnti-IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PB9223&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9223-5-IHC-anti-irs1-picoband-antibody.jpgAnti-IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PB9223&#44; IHC(P)<br>IHC(P): Rat Skeletal Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9223-6_1-IHC-anti-irs1-picoband-antibody.jpgAnti-IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PB9223&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9223-7_1-IHC-anti-irs1-picoband-antibody.jpgAnti-IRS1 Antibody Picoband&reg;Anti-IRS1 antibody&#44; PB9223&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9223-fsn3-13-e70736-g003.jpgAnti-IRS1 Antibody Picoband&reg;EGCG relieved senescence, IR, and lipid accumulation in the MPC5 model cells. (A) Molecular docking predicts the interaction of EGCG with SIRT1. (B) Determination of the optimal concentration of D‐galactose, PA, D‐galactose combined with PA and EGCG. n = 6. (C) The treatment of cell modeling in each group. (D, E) Representative SA‐β‐gal staining images of MPC5 cells and the ratio of SA‐β‐gal‐positive cells. 400×, n = 3. (F, G) Representative Oil Red O images of MPC5 cells and quantitative analysis of positive area. 400×, n = 3. (H) Representative WB images and quantification of the level of P21, P53, and P‐IRS1. n = 3. Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05. * symbol that the cell viability (%) of different group compared with the group of without any reagents, * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9223-fsn3-13-e70736-g005.jpgAnti-IRS1 Antibody Picoband&reg;Effects of TP on the typical symptoms, IR, and senescence in the aged T2DM model rats. (A) Body weight ( n = 10). (B) Food intake ( n = 10). (C) 24 h urine volume ( n = 10). (D) 24 h water intake ( n = 10). (E, F) Representative SA‐β‐gal staining images in kidney tissues of rats and the area of SA‐β‐gal staining positive cells (100×, n = 3). (G, H) Representative HE images and quantitative analysis of islet vacuoles ratio to cells (200×, n = 3). (I) FBG ( n = 10). (J–M) Representative WB images and quantification of the expression of P21, P53, and P‐IRS1 ( n = 6). Compared with the CON group, a p < 0.05; compared with the MOD group, b p < 0.05; compared with the TP‐L group, c p < 0.05; compared with the TP‐M group, d p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12339905/'>40801050</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mum1-picoband-trade-antibody-pb9222-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9222-1-WB-anti-mum1-picoband-antibody.jpgAnti-MUM1/IRF4 Antibody Picoband&reg;Anti-MUM1 antibody&#44; PB9222&#44; Western blotting<br>All lanes: Anti MUM1 (PB9222) at 0.5ug/ml<br>WB: Recombinant Human MUM1 Protein 0.5ng<br>Predicted bind size: 38KD<br>Observed bind size: 38KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9222-2-WB-anti-mum1-picoband-antibody.jpgAnti-MUM1/IRF4 Antibody Picoband&reg;Anti-MUM1 antibody&#44; PB9222&#44; Western blotting<br>All lanes: Anti MUM1 (PB9222) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 51KD<br>Observed bind size: 40KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9222-3-IHC-anti-mum1-picoband-antibody.jpgAnti-MUM1/IRF4 Antibody Picoband&reg;Anti-MUM1 antibody&#44; PB9222&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gata5-picoband-trade-antibody-pb9200-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9200-gata5-primary-antibodies-wb-testing-1.jpgAnti-GATA5 Antibody Picoband&reg; Western blot analysis of GATA5 using anti-GATA5 antibody (PB9200). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA5 antigen affinity purified polyclonal antibody (Catalog # PB9200) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA5 at approximately 50 kDa. The expected band size for GATA5 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gclc-picoband-trade-antibody-pb9201-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9201-gclc-primary-antibodies-wb-testing-1.jpgAnti-GCLC Antibody Picoband&reg; Western blot analysis of GCLC using anti-GCLC antibody (PB9201). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLC antigen affinity purified polyclonal antibody (Catalog # PB9201) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCLC at approximately 73 kDa. The expected band size for GCLC is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9201-gclc-primary-antibodies-ihc-testing-2.jpgAnti-GCLC Antibody Picoband&reg; IHC analysis of GCLC using anti-GCLC antibody (PB9201). <br> GCLC was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GCLC Antibody (PB9201) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9201-gclc-primary-antibodies-fcm-testing-3.jpgAnti-GCLC Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-GCLC antibody (PB9201). <br> Overlay histogram showing A549 cells stained with PB9201 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GCLC Antibody (PB9201, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikk-beta-picoband-trade-antibody-pb9221-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9221-1-WB-anti-ikk-beta-picoband-antibody.jpgAnti-IKK beta/IKBKB Antibody Picoband&reg;Anti-IKK beta antibody&#44; PB9221&#44; Western blotting<br>All lanes: Anti IKK beta (PB9221) at 0.5ug/ml<br>Lane 1: HEPG2 Whole Cell Lysate at 40ug<br>Lane 2: COLO320 Whole Cell Lysate at 40ug<br>Lane 3: M231 Whole Cell Lysate at 40ug<br>Lane 4: HT1080 Whole Cell Lysate at 40ug<br>Predicted bind size: 87KD<br>Observed bind size: 87KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9221-2-IHC-anti-ikk-beta-picoband-antibody.jpgAnti-IKK beta/IKBKB Antibody Picoband&reg;Anti-IKK beta antibody&#44; PB9221&#44;IHC(P)<br> IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9221-3-WB-anti-ikk-beta-picoband-antibody.jpgAnti-IKK beta/IKBKB Antibody Picoband&reg;Anti-IKK beta antibody&#44; PB9221&#44; Western blotting<br>All lanes: Anti IKK beta (PB9221) at 0.5ug/ml<br>WB: Recombinant Human IKK beta Protein 0.5ng<br>Predicted bind size: 43KD<br>Observed bind size: 43KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9221-ikk-beta-primary-antibodies-if-testing-4.jpgAnti-IKK beta/IKBKB Antibody Picoband&reg; IF analysis of IKK beta using anti-IKK beta antibody (PB9221). <br> IKK beta was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IKK beta Antibody (PB9221) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9221-ikk-beta-primary-antibodies-fcm-testing-5.jpgAnti-IKK beta/IKBKB Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-IKK beta antibody (PB9221).<br>Overlay histogram showing 293T cells stained with PB9221 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IKK beta Antibody (PB9221,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hrg-picoband-trade-antibody-pb9220-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9220-1-WB-anti-hrg-hprg-picoband-antibody.jpgAnti-HRG Antibody Picoband&reg;Anti-HRG antibody&#44; PB9220&#44; Western blotting<br>All lanes: Anti HRG (PB9220) at 0.5ug/ml<br>WB: Recombinant Human HRG Protein 0.5ng<br>Predicted bind size: 40KD<br>Observed bind size: 40KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9220-2-WB-anti-hrg-hprg-picoband-antibody.jpgAnti-HRG Antibody Picoband&reg;Anti-HRG antibody&#44; PB9220&#44; Western blotting<br>All lanes: Anti HRG (PB9220) at 0.5ug/ml<br>WB: Human Placenta Tissue Lysate at 50ug<br>Predicted bind size: 60KD<br>Observed bind size: 60KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9220-3-IHC-anti-hrg-hprg-picoband-antibody.jpgAnti-HRG Antibody Picoband&reg;Anti-HRG antibody&#44; PB9220&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hoxa11-picoband-trade-antibody-pb9218-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9218-1-WB-anti-hoxa11-picoband-antibody.jpgAnti-HOXA11 Antibody Picoband&reg; Western blot analysis of HOXA11 using anti-HOXA11 antibody (PB9218). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> lane 1: Recombinant Human HOXA11 Protein 0.5ng. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody (Catalog # PB9218) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 38KD. The expected band size for HOXA11 is at 38KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9218-2-WB-anti-hoxa11-picoband-antibody.jpgAnti-HOXA11 Antibody Picoband&reg; Western blot analysis of HOXA11 using anti-HOXA11 antibody (PB9218). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44; <br> Lane 2: Human Placenta Tissue Lysate&#44; <br> Lane 3: HLEA Whole Cell Lysate&#44; <br> Lane 4: HT1080 Whole Cell Lysate&#44; <br> Lane 5: HEPA Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody (Catalog # PB9218) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 35KD. The expected band size for HOXA11 is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9218-hoxa11-primary-antibodies-fc-testing-3.jpgAnti-HOXA11 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-HOXA11 antibody (PB9218). <br>Overlay histogram showing U937 cells stained with PB9218 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXA11 Antibody (PB9218&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hoxa10-picoband-trade-antibody-pb9217-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9217-1-WB-anti-hoxa10-picoband-antibody.jpgAnti-HOXA10 Antibody Picoband&reg;Anti-HOXA10 antibody&#44; PB9217&#44; Western blotting<br>All lanes: Anti HOXA10 (PB9217) at 0.5ug/ml<br>WB: Recombinant Human HOXA10 Protein 0.5ng<br>Predicted bind size: 45KD<br>Observed bind size: 45KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9217-2-WB-anti-hoxa10-picoband-antibody.jpgAnti-HOXA10 Antibody Picoband&reg;Anti-HOXA10 antibody&#44; PB9217&#44; Western blotting<br>All lanes: Anti HOXA10 (PB9217) at 0.5ug/ml<br>Lane 1: Rat Kidney Tissue Lysate at 50ug<br>Lane 2: Human Placenta Tissue Lysate at 50ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Lane 4: SW620 Whole Cell Lysate at 40ug<br>Lane 5: HEPA Whole Cell Lysate at 40ug<br>Predicted bind size: 41KD<br>Observed bind size: 41KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gfra1-picoband-trade-antibody-pb9202-boster.html2026-03-24T05:04:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9202-1-WB-anti-gfra1-gfr-alpha-1-picoband-antibody.jpgAnti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband&reg; Western blot analysis of GFRA1 using anti-GFRA1 antibody (PB9202). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human GFRA1 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFRA1 antigen affinity purified polyclonal antibody (Catalog # PB9202) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFRA1 at approximately 39 kDa. The expected band size for GFRA1 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9202-2-WB-anti-gfra1-gfr-alpha-1-picoband-antibody.jpgAnti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband&reg; Western blot analysis of GFRA1 using anti-GFRA1 antibody (PB9202). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFRA1 antigen affinity purified polyclonal antibody (Catalog # PB9202) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFRA1 at approximately 51 kDa. The expected band size for GFRA1 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9202-12864_2024_10120_fig2_html.pngAnti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband&reg;Effects of EZH2 interference or overexpression and JMJD3 interference or overexpression on self-renewal, proliferation and differentiation of spermatogonia. ( A ) The mRNA levels of PCNA, Cyclin-A, GFRA1, PLZF and C-KIT related to spermatogonia self-renewal and proliferation were changed after EZH2 and JMJD3 knockdown. ( B ) The expression of PCNA, cyclin-A, GFRA1, PLZF, C-KIT, DAZL and VASA was detected by qRT-PCR after EZH2 and JMJD3 overexpression. ( C ) The protein expression changes as well as statistical analysis of PCNA, DAZL and GFRA1 after EZH2 and JMJD3 overexpression. The membrane is lysed prior to hybridization with the antibody and the image has been cropped for a more aesthetically pleasing display. The full- length blots can be obtained from Additional file 2: Fig . ( D ) The cell cycle of EZH2 and JMJD3 overexpression cells was detected by flow cytometry. ( E ) Protein interaction network of EZH2, JMJD3 and spermatogonia self-renewal, proliferation and differentiation-related genes <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12864-024-10120-9'>38424516</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9202-fphys-13-843825-g001.jpgAnti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband&reg;Relative expression of specific genes in spermatogonial cells. (A) The mRNA expression of TET1 in spermatogonial cells was detected by QRT-PCR. (B) The mRNA expression of PLZF in spermatogonial cells was detected by QRT-PCR. (C) The mRNA expression of GFRα1 in spermatogonial cells was detected by QRT-PCR. (D) The mRNA expression of PRDM1 in spermatogonial cells was detected by QRT-PCR. (E) The mRNA expression of MAGE4 in spermatogonial cells was detected by QRT-PCR. (F) The expression of PLZF in TET1 overexpressed cells was detected by Western Blot. (G) Quantification of PLZF protein levels in TET1 overexpressed cells. (H) The expression of GFRα1 in TET1 overexpressed cells was detected by Western Blot. (I) Quantification of GFRα1 protein levels in TET1 overexpressed cells. p < .0001(****), p < .001(***), p < .01(**), p < .05(*).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.843825/full'>35222097</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9202-3-IHC-anti-gfra1-gfr-alpha-1-picoband-antibody.jpgAnti-GDNF Receptor alpha 1/GFRA1 Antibody Picoband&reg; IHC analysis of GFRA1 using anti-GFRA1 antibody (PB9202). <br> GFRA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GFRA1 Antibody (PB9202) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hoxa9-picoband-trade-antibody-pb9216-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9216-1-WB-anti-hoxa9-picoband-antibody.jpgAnti-HOXA9 Antibody Picoband&reg;Anti-HOXA9 antibody&#44; PB9216&#44; Western blotting<br>All lanes: Anti HOXA9 (PB9216) at 0.5ug/ml<br>WB: Recombinant Human HOXA9 Protein 0.5ng<br>Predicted bind size: 38KD<br>Observed bind size: 38KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9216-2-WB-anti-hoxa9-picoband-antibody.jpgAnti-HOXA9 Antibody Picoband&reg;Anti-HOXA9 antibody&#44; PB9216&#44; Western blotting<br>All lanes: Anti HOXA9 (PB9216) at 0.5ug/ml<br>Lane 1: Rat Testis Tissue Lysate at 50ug<br>Lane 2: HEPG2 Whole Cell Lysate at 40ug<br>Lane 3: HEPA Whole Cell Lysate at 40ug<br>Predicted bind size: 30KD<br>Observed bind size: 30KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hnf1-picoband-trade-antibody-pb9214-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9214-hnf1-primary-antibodies-wb-testing-1.jpgAnti-HNF1/HNF1A Antibody Picoband&reg; Western blot analysis of HNF1/HNF1A using anti-HNF1/HNF1A antibody (PB9214). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1/HNF1A antigen affinity purified polyclonal antibody (Catalog # PB9214) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1/HNF1A at approximately 80 kDa. The expected band size for HNF1/HNF1A is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmox2-picoband-trade-antibody-pb9213-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9213-41598_2019_43347_fig5_html.pngAnti-Heme oxygenase 2/HMOX2 Antibody Picoband&reg;TLR4 is a negative regulator of biliverdin/BVRA signaling. ( a ) Healthy donor’s blood was untreated (lane 1) or treated with LPS (10 ng/ml) for the indicated times (lanes 2–7). Leukocytes were isolated and analyzed. Abbreviations are: Heme oxygenase 1, HO-1; Heme oxygenase 2, HO-2; Haptoglobin, Hapto. ( b ) In an earlier study , subjects were administered LPS (1 ng/kg) in vivo and blood was drawn at the indicated times post LPS infusion. Leukocyte lysates available from that study were normalized for protein content and analyzed by western blotting. ( c – f ) In another prior study , leukocytes from four subjects administered LPS in vivo were analyzed for changes in gene expression over a period of 24 hours post LPS infusion. Data from that study , available through GEO dataset GSE3284, revealed a temporal ( c ) increase in TLR4 mRNA, ( d ) decline in BVRA mRNA, ( e ) decrease in PKCζ mRNA ( f ) increase in haptoglobin mRNA expression. In ( c – f ) each symbol represents a subject. ( g ) At time 0 (0 hr) healthy donor’s blood was untreated (UN; lane 1), treated for 1 hour with biliverdin (Bili 0 hr; 50 μM; lane 2), treated for 4 hours with LPS (10 ng/ml; lanes 4–6) to trigger a decline in BVRA expression, or for 1 hour with metformin (Met; 10 μM; lane 7). Four hours later (time 4 hr) blood samples were treated for 1 hour with biliverdin (Bili 4 hr; 50 μM; lane 3) or metformin (Met 4 hr; 10 μM; lane 8). Samples pretreated with LPS for 4 hours (lanes 4–6), were then treated for 1 hr with biliverdin (Bili; 50 μM; lane 5) or metformin (Met; 10 μM; lane 6). Leukocytes were isolated and analyzed by western blotting. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-019-43347-8'>31065010</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9213-hmox2-primary-antibodies-wb-testing-1.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband&reg;Western blot analysis of PHMOX2 using anti-HMOX2 antibody (PB9213). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: huamn Jurkat whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human PANC-1 whole cell lysates, <br> Lane 5: rat PC-12 whole cell lysates, <br> Lane 6: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX2 antigen affinity purified polyclonal antibody (PB9213) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HMOX2 at approximately 36 kDa. The expected band size for HMOX2 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9213-hmox2-primary-antibodies-ihc-testing-1.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband&reg;IHC analysis of HMOX2 using anti-HMOX2 antibody (PB9213). <br> HMOX2 was detected in a paraffin-embedded section of human appendix mucinous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX2 Antibody (PB9213) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9213-hmox2-primary-antibodies-ihc-testing-2.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband&reg;IHC analysis of HMOX2 using anti-HMOX2 antibody (PB9213). <br> HMOX2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX2 Antibody (PB9213) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9213-hmox2-primary-antibodies-ihc-testing-3.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband&reg;IHC analysis of HMOX2 using anti-HMOX2 antibody (PB9213). <br> HMOX2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX2 Antibody (PB9213) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9213-hmox2-primary-antibodies-ihc-testing-4.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband&reg;IHC analysis of HMOX2 using anti-HMOX2 antibody (PB9213). <br> HMOX2 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX2 Antibody (PB9213) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9213-hmox2-primary-antibodies-ihc-testing-5.jpgAnti-Heme oxygenase 2/HMOX2 Antibody Picoband&reg;IHC analysis of HMOX2 using anti-HMOX2 antibody (PB9213). <br> HMOX2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX2 Antibody (PB9213) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmox1-picoband-trade-antibody-pb9212-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9212-hmox1-primary-antibodies-ihc-testing-3.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg;IHC analysis of HO-1/HMOX1 using anti-HO-1/HMOX1 antibody (PB9212). <br>HO-1/HMOX1 was detected in a paraffin-embedded section of human small intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HO-1/HMOX1 Antibody (PB9212) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9212-hmox1-primary-antibodies-wb-testing-3.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg; Western blot analysis of heme oxygenase 1/HMOX1 using anti-heme oxygenase 1/HMOX1 antibody (PB9212). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 1x PBS +0.2% Tween-20 + 10% nonfat dry milk for 1.5 hour at RT. The membrane was incubated with rabbit anti-heme oxygenase 1/HMOX1 antibody (PB9212) at 0.5 μg/mL overnight at room temperature for 3 hours, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP-conjugated anti rabbit antibodies at 4℃ for 12 hours. The signal is developed using an Enhanced chemiluminescence (Thermo Fisher) for one minute. A specific band was detected for heme oxygenase 1/HMOX1 at approximately 32 kDa. The expected band size for heme oxygenase 1/HMOX1 is at 32 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9212-hmox1-primary-antibodies-wb-testing-1.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg; Western blot analysis of HMOX1 using anti-HMOX1 antibody (PB9212). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMOX1 antigen affinity purified polyclonal antibody (Catalog # PB9212) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMOX1 at approximately 33 kDa. The expected band size for HMOX1 is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9212-hmox1-primary-antibodies-ihc-testing-2.jpgAnti-Heme Oxygenase 1/HMOX1 Antibody Picoband&reg; IHC analysis of HMOX1 using anti-HMOX1 antibody (PB9212). <br> HMOX1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HMOX1 Antibody (PB9212) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gpx1-picoband-trade-antibody-pb9203-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9203-gpx1-primary-antibodies-wb-testing-1.jpgAnti-Glutathione Peroxidase 1/GPX1 Antibody Picoband&reg; Western blot analysis of GPX1 using anti-GPX1 antibody (PB9203). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: rat lung tissue lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: mouse lung tissue lysates,<br> Lane 5: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 antigen affinity purified polyclonal antibody (Catalog # PB9203) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22 kDa. The expected band size for GPX1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9203-gpx1-primary-antibodies-ihc-testing-2.jpgAnti-Glutathione Peroxidase 1/GPX1 Antibody Picoband&reg; IHC analysis of GPX1 using anti-GPX1 antibody (PB9203). <br> GPX1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPX1 Antibody (PB9203) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9203-gpx1-primary-antibodies-ihc-testing-3.jpgAnti-Glutathione Peroxidase 1/GPX1 Antibody Picoband&reg; IHC analysis of GPX1 using anti-GPX1 antibody (PB9203). <br> GPX1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GPX1 Antibody (PB9203) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hexb-picoband-trade-antibody-pb9211-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9211-hexb-primary-antibodies-wb-testing-1.jpgAnti-HEXB Antibody Picoband&reg; Western blot analysis of HEXB using anti-HEXB antibody (PB9211). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEXB antigen affinity purified polyclonal antibody (Catalog # PB9211) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEXB at approximately 63 kDa. The expected band size for HEXB is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9211-3-IHC-anti-hexb-picoband-antibody.jpgAnti-HEXB Antibody Picoband&reg;Anti-HEXB antibody&#44; PB9211&#44;IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gria1-picoband-trade-antibody-pb9204-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9204-1_1-WB-anti-gria1-glur1-picoband-antibody.jpgAnti-Glutamate Receptor 1/GRIA1 Antibody Picoband&reg; Western blot analysis of GRIA1 using anti-GRIA1 antibody (PB9204). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> lane 1: Recombinant Human GRIA1 Protein 0.5ng. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA1 antigen affinity purified polyclonal antibody (Catalog # PB9204) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA1 at approximately 40KD. The expected band size for GRIA1 is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9204-gria1-primary-antibodies-ihc-testing-7.jpgAnti-Glutamate Receptor 1/GRIA1 Antibody Picoband&reg;IHC analysis of GLUR1/GRIA1 using anti-GLUR1/GRIA1 antibody (PB9204). <br>GLUR1/GRIA1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GLUR1/GRIA1 Antibody (PB9204) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9204-2_1-WB-anti-gria1-glur1-picoband-antibody.jpgAnti-Glutamate Receptor 1/GRIA1 Antibody Picoband&reg; Western blot analysis of GRIA1 using anti-GRIA1 antibody (PB9204). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44; <br> Lane 2: Mouse Brain Tissue Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA1 antigen affinity purified polyclonal antibody (Catalog # PB9204) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA1 at approximately 101KD. The expected band size for GRIA1 is at 101KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9204-4_1-IHC-anti-gria1-glur1-picoband-antibody.jpgAnti-Glutamate Receptor 1/GRIA1 Antibody Picoband&reg; IHC analysis of GRIA1 using anti-GRIA1 antibody (PB9204).<br> GRIA1 was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIA1 Antibody (PB9204) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9204-3_1-IHC-anti-gria1-glur1-picoband-antibody.jpgAnti-Glutamate Receptor 1/GRIA1 Antibody Picoband&reg; IHC analysis of GRIA1 using anti-GRIA1 antibody (PB9204).<br> GRIA1 was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIA1 Antibody (PB9204) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9204-5_1-IHC-anti-gria1-glur1-picoband-antibody.jpgAnti-Glutamate Receptor 1/GRIA1 Antibody Picoband&reg; IHC analysis of GRIA1 using anti-GRIA1 antibody (PB9204).<br> GRIA1 was detected in paraffin-embedded section of Human Meningeoma Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIA1 Antibody (PB9204) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9204-slc6a1-primary-antibodies-ihc-testing-6.jpgAnti-Glutamate Receptor 1/GRIA1 Antibody Picoband&reg;IHC analysis of SLC6A1 using anti-SLC6A1 antibody (PB9204). <br> SLC6A1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SLC6A1 Antibody (PB9204) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hcn1-picoband-trade-antibody-pb9210-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9210-hcn1-primary-antibodies-wb-testing-1.jpgAnti-HCN1 Antibody Picoband&reg; Western blot analysis of HCN1 using anti-HCN1 antibody (PB9210). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human U20S whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCN1 antigen affinity purified polyclonal antibody (Catalog # PB9210) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN1 at approximately 120 kDa. The expected band size for HCN1 is at 99 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gelsolin-picoband-trade-antibody-pb9209-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9209-gelsolin-primary-antibodies-wb-testing-1.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; Western blot analysis of Gelsolin using anti-Gelsolin antibody (PB9209). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human THP-1 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: monkey COS-7 whole cell lysates,<br> Lane 5: monkey kidney tissue lysates,<br> Lane 6: human CACO-2 whole cell lysates,<br> Lane 7: rat PC-12 whole cell lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gelsolin antigen affinity purified polyclonal antibody (Catalog # PB9209) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gelsolin at approximately 86 kDa. The expected band size for Gelsolin is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9209-2-ihc-anti-gelsolin-picoband-antibody.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IHC analysis of Gelsolin using anti-Gelsolin antibody (PB9209). <br> Gelsolin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Gelsolin Antibody (PB9209) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9209-3-IHC-anti-gelsolin-picoband-antibody.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IHC analysis of Gelsolin using anti-Gelsolin antibody (PB9209). <br> Gelsolin was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Gelsolin Antibody (PB9209) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9209-4-IHC-anti-gelsolin-picoband-antibody.jpgAnti-Gelsolin/GSN Antibody Picoband&reg; IHC analysis of Gelsolin using anti-Gelsolin antibody (PB9209). <br> Gelsolin was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Gelsolin Antibody (PB9209) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grik1-picoband-trade-antibody-pb9208-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9208-1-WB-anti-grik1-picoband-antibody.jpgAnti-GRIK1 Antibody Picoband&reg; Western blot analysis of GRIK1 using anti-GRIK1 antibody (PB9208). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human GRIK1 protein 0.5 ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody (Catalog # PB9208) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 40 kDa. The expected band size for GRIK1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9208-2-WB-anti-grik1-picoband-antibody.jpgAnti-GRIK1 Antibody Picoband&reg; Western blot analysis of GRIK1 using anti-GRIK1 antibody (PB9208). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse brain tissue lysates, <br> Lane 2: mouse brain tissue lysates, <br> Lane 3: human SHG whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody (Catalog # PB9208) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 104 kDa. The expected band size for GRIK1 is at 104 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9208-3.jpgAnti-GRIK1 Antibody Picoband&reg; Western blot analysis of GRIK1 using anti-GRIK1 antibody (PB9208). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody (Catalog # PB9208) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 104KD. The expected band size for GRIK1 is at 104KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9208-4.jpgAnti-GRIK1 Antibody Picoband&reg; IHC analysis of GRIK1 using anti-GRIK1 antibody (PB9208). <br> GRIK1 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIK1 Antibody (PB9208) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9208-5.jpgAnti-GRIK1 Antibody Picoband&reg; IHC analysis of GRIK1 using anti-GRIK1 antibody (PB9208). <br> GRIK1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIK1 Antibody (PB9208) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9208-6.jpgAnti-GRIK1 Antibody Picoband&reg; IHC analysis of GRIK1 using anti-GRIK1 antibody (PB9208). <br> GRIK1 was detected in paraffin-embedded section of human thyroid cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIK1 Antibody (PB9208) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9208-7.jpgAnti-GRIK1 Antibody Picoband&reg; IHC analysis of GRIK1 using anti-GRIK1 antibody (PB9208). <br> GRIK1 was detected in paraffin-embedded section of mouse brain tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIK1 Antibody (PB9208) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gria2-picoband-trade-antibody-pb9205-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9205-gria2-primary-antibodies-wb-testing-1.jpgAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg;Western blot analysis of GRIA2 using anti-GRIA2 antibody (PB9205). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA2 antigen affinity purified polyclonal antibody (PB9205) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRIA2 at approximately 99 kDa. The expected band size for GRIA2 is at 99 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9205-gria2-primary-antibodies-ihc-testing-4.jpgAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg;IHC analysis of Glutamate receptor 2/GRIA2 using anti-Glutamate receptor 2/GRIA2 antibody (PB9205). <br>Glutamate receptor 2/GRIA2 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Glutamate receptor 2/GRIA2 Antibody (PB9205) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9205-3-IHC-anti-gria2-glur2-picoband-antibody.jpgAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg; IHC analysis of GRIA2 using anti-GRIA2 antibody (PB9205). <br> GRIA2 was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIA2 Antibody (PB9205) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9205-4-IHC-anti-gria2-glur2-picoband-antibody.jpgAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg; IHC analysis of GRIA2 using anti-GRIA2 antibody (PB9205). <br> GRIA2 was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GRIA2 Antibody (PB9205) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9205-4_1.jpgAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg; IF analysis of GRIA2 using anti-GRIA2 antibody (PB9205) <br> GRIA2 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-GRIA2 Antibody (PB9205) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9205-gria2-primary-antibodies-ihc-testing-6.jpgAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg; IHC analysis of GRIA2 using anti-GRIA2 antibody (PB9205). <br> GRIA23 was detected in a frozen section of rat brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GRIA2 Antibody (PB9205) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9205-gria2-primary-antibodies-if-testing-7.jpgAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg; IF analysis of GRIA2 using anti-GRIA2 antibody (PB9205). <br> GRIA2 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/mL rabbit anti-GRIA2 Antibody (PB9205) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9205-5.pngAnti-Ionotropic Glutamate receptor 2/GRIA2 Antibody Picoband&reg; Flow Cytometry analysis of U-87MG cells using anti-GRIA2 antibody (PB9205). <br> Overlay histogram showing U-87MG cells stained with PB9205 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA2 Antibody (PB9205&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gria4-picoband-trade-antibody-pb9207-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9207-gria4-primary-antibodies-wb-testing-1.jpgAnti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband&reg;Western blot analysis of GRIA4 using anti-GRIA4 antibody (PB9207). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 2: rat C6 whole cell lysates,<br> Lane 2: mouse brain tissue lysates,<br> Lane 2: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA4 antigen affinity purified polyclonal antibody (PB9207) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRIA4 at approximately 101 kDa. The expected band size for GRIA4 is at 101 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9207-2.pngAnti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-GRIA4 antibody (PB9207). <br> Overlay histogram showing A549 cells stained with PB9207 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRIA4 Antibody (PB9207&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gria3-picoband-trade-antibody-pb9206-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9206-gria3-primary-antibodies-wb-testing-1.jpgAnti-Glutamate receptor 3/GRIA3 Antibody Picoband&reg;Western blot analysis of GRIA3 using anti-GRIA3 antibody (PB9206). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA3 antigen affinity purified polyclonal antibody (PB9206) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRIA3 at approximately 101 kDa. The expected band size for GRIA3 is at 101 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-16-picoband-trade-antibody-pb9238-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9238-1-WB-anti-il-16-antibody.jpgAnti-IL16 Antibody Picoband&reg;Anti-IL-16 antibody&#44; PB9238&#44; Western blotting<br>All lanes: Anti IL-16 (PB9238) at 0.5ug/ml<br>WB: Recombinant Human IL-16 Protein 0.5ng<br>Predicted bind size: 17KD<br>Observed bind size: 17KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9238-2-IHC-anti-il-16-antibody.jpgAnti-IL16 Antibody Picoband&reg;Anti-IL-16 antibody&#44; PB9238&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9238-3-IF-anti-il-16-antibody.jpgAnti-IL16 Antibody Picoband&reg; IHC analysis of IL16 using anti-IL16 antibody (PB9238).<br> IL16 was detected in immunocytochemical section of human cord blood. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-IL16 Antibody (PB9238) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9238-4.jpgAnti-IL16 Antibody Picoband&reg; Western blot analysis of IL16 using anti-IL16 antibody (PB9238). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human U937 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL16 antigen affinity purified polyclonal antibody (Catalog # PB9238) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL16 at approximately 45-55KD. The expected band size for IL16 is at 142KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf1-picoband-trade-antibody-pb9241-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9241-1-WB-anti-fgf1-fgf-acidic-picoband-antibody.jpgAnti-FGF1 Antibody Picoband&reg;Anti-FGF1 antibody&#44; PB9241&#44; Western blotting<br>All lanes: Anti FGF1 (PB9241) at 0.5ug/ml<br>WB: Recombinant Human FGF1 Protein 0.5ng<br>Predicted bind size: 36KD<br>Observed bind size: 36KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fetuin-a-picoband-trade-antibody-pb9242-boster.html2026-03-24T05:04:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9242-ahsg-primary-antibodies-wb-testing-1.jpgAnti-Fetuin A/AHSG Antibody Picoband&reg; Western blot analysis of Fetuin A/AHSG using anti-Fetuin A/AHSG antibody (PB9242). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse plasma lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fetuin A/AHSG antigen affinity purified polyclonal antibody (Catalog # PB9242) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fetuin A/AHSG at approximately 55-60 kDa. The expected band size for Fetuin A/AHSG is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rack1-picoband-trade-antibody-pb9243-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9243-rack1-primary-antibodies-wb-testing-1.jpgAnti-RACK1 GNB2L1 Antibody Picoband&reg; Western blot analysis of RACK1 using anti-RACK1 antibody (PB9243). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human Raji whole cell lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates,<br> Lane 8: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RACK1 antigen affinity purified polyclonal antibody (Catalog # PB9243) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RACK1 at approximately 36 kDa and for RACK1 mature form at approximately 32 kDa. The expected band size for RACK1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9243-3_1.jpgAnti-RACK1 GNB2L1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-RACK1 antibody (PB9243).<br>Overlay histogram showing A431 cells stained with PB9243 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RACK1 Antibody (PB9243,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9243-2_1.jpgAnti-RACK1 GNB2L1 Antibody Picoband&reg; IF analysis of RACK1 using anti-RACK1 antibody (PB9243). <br> RACK1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RACK1 Antibody (PB9243) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-15-picoband-trade-antibody-pb9244-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9244-1-WB-anti-il-15-antibody.jpgAnti-IL15 Antibody Picoband&reg;Anti-IL-15 antibody&#44; PB9244&#44; Western blotting<br>All lanes: Anti IL-15 (PB9244) at 0.5ug/ml<br>WB: Recombinant Human IL-15 Protein 0.5ng<br>Predicted bind size: 15KD<br>Observed bind size: 15KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9244-12934_2021_1605_fig1_html.pngAnti-IL15 Antibody Picoband&reg;Construction and expression of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. a Schematic diagram of the two IL-15/SuIL-15Rα-IgG4 Fc complexes. IL-15/SuIL-15Rα-dFc was composed of two molecules of mutated IL-15 noncovalently bound to a modified dimeric SuIL-15Rα-IgG4 Fc fusion protein, while IL-15/SuIL-15Rα-mFc consisted of a single molecule of mutated IL-15 noncovalently bound to a modified monomeric SuIL-15Rα-IgG4 Fc fusion protein. b The construction strategy of the two IL-15/SuIL-15Rα-IgG4 Fc complexes. pPIC9-SuIL-15Rα-dFc or pPIC9-SuIL-15Rα-mFc and pPICZα-IL-15 were inserted into the genome of GS115 through homologous recombination using the his4 and AOX1 sequences, respectively. c Screening for the expression of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. Expression clones were first screened by dot blotting using rabbit anti-human IL-15 antibody followed by donkey anti-rabbit IgG-HRP conjugate, and then high expression clones were further selected and confirmed by Western blotting under nonreducing conditions using anti-human IgG4-HRP conjugate. Clones No. 15–2 and 62–4 were selected as the expression clones used in pilot-scale fermentation of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc, respectively. The numbers in the figure represent the clone numbers <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12934-021-01605-3'>34107983</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9244-12934_2021_1605_fig2_html.pngAnti-IL15 Antibody Picoband&reg;Pilot-scale fermentation, purification and characterization of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. a Representative three-step fermentation process, including batch phase, glycerol fed-batch phase and methanol induction phase. Parameters, such as dissolved oxygen (DO), feeding speed, temperature and pH, were monitored during fermentation. b Cell growth of the two IL-15/SuIL-15Rα-IgG4 Fc complex-expressing strains during fermentation was monitored and represented as wet cell weight. c The expression of the two IL-15/SuIL-15Rα-IgG4 Fc complexes during fermentation was analyzed by Western blotting using an anti-human IgG4-HRP conjugate under nonreducing conditions. d The downstream processing workflow of fermentation broth. e The two purified IL-15/SuIL-15Rα-IgG4 Fc complexes were separated by SDS-PAGE under reducing conditions and identified by Western blotting or Coomassie blue staining. The contents within the dashed boxes are the presumed protein structure of the corresponding bands. M: prestained protein marker; dFc: IL-15/SuIL-15Rα-dFc; mFc: IL-15/SuIL-15Rα-mFc <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12934-021-01605-3'>34107983</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9244-12934_2021_1605_fig3_html.pngAnti-IL15 Antibody Picoband&reg;In vitro bioactivity of rhIL-15, IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. A CTLL-2 cell proliferation assay was used to evaluate the biological activity of the rhIL-15 and IL-15/SuIL-15Rα-IgG4 Fc complexes. The EC 50 values were calculated using the four-parameter nonlinear logistic regression model. All data points are the means ± standard deviation of triplicate OD values. The results are representative of at least three experiments <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12934-021-01605-3'>34107983</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9244-12934_2021_1605_fig4_html.pngAnti-IL15 Antibody Picoband&reg;Pharmacokinetics analysis of rhIL-15, IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. C57BL/6 J mice (male, 6–8 weeks of age, n = 4–9 for each group) were intravenously administered 0.28 mg/kg rhIL-15 or 1 mg/kg IL-15/SuIL-15Rα-IgG4 Fc complexes, and blood samples were collected at the indicated time points after injection. The concentration of IL-15 in serum was measured by ELISA. All data points are the means ± standard deviation of the concentrations <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12934-021-01605-3'>34107983</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9244-12934_2021_1605_fig5_html.pngAnti-IL15 Antibody Picoband&reg;In vivo bioactivity of rhIL-15, IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. C57BL/6 J mice (male, 6–8 weeks of age, n = 10–14 for each group) were intravenously injected with PBS, 0.28 mg/kg rhIL-15 or 1 mg/kg IL-15/SuIL-15Rα-IgG4 Fc complexes. Seventy-two hours after treatment, spleens and blood samples were collected and prepared for flow cytometry. a Representative photo of spleens separated from each group (n = 5). b Spleen weight statistics. c The percentage of each indicated cell subset in splenocytes. d The percentage of each indicated cell subset in peripheral blood cells. The data shown in b, c, and d are combined from three independent experiments, and all data points are the means ± standard deviation. ns, not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001 and ****: p < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12934-021-01605-3'>34107983</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9244-12934_2021_1605_fig6_html.pngAnti-IL15 Antibody Picoband&reg;Effects of rhIL-2, rhIL-15, IL-15/SuIL-15Rα-dFc, and IL-15/SuIL-15Rα-mFc on NK and CD8 + T cell proliferation. Human PBMCs from healthy donors (n = 6) were cultured for 7 days in the absence or presence of 50 IU/mL rhIL-2, 10 ng/mL rhIL-15 or 35.7 ng/mL IL-15/SuIL-15Rα-IgG4 Fc complexes, and the proliferation of CFSE + NK cells and CFSE + CD8 + T cells was measured by flow cytometry. a Results from one representative healthy donor. b Statistics on the proliferation of NK cells or CD8 + T cells from all healthy donors. All data points are means ± standard deviation. *: p < 0.05; **: p < 0.01; ***: p < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12934-021-01605-3'>34107983</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-18-picoband-trade-antibody-pb9245-boster.html2026-03-25T05:22:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9245-1-WB-anti-il-18-antibody.jpgAnti-IL18 Antibody Picoband&reg;Anti-IL-18 Picoband antibody&#44; PB9245&#44; Western blotting<br>All lanes: Anti IL-18 (PB9245) at 0.5ug/ml<br>WB: Recombinant Mouse IL-18 Protein 0.5ng<br>Predicted bind size: 22KD<br>Observed bind size: 22KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9245-2-WB-anti-il-18-antibody.jpgAnti-IL18 Antibody Picoband&reg;Anti-IL-18 Picoband antibody&#44; PB9245&#44; Western blotting<br>All lanes: Anti IL-18 (PB9245) at 0.5ug/ml<br>WB: NIH3T3 Whole Cell Lysate at 40ug<br>Predicted bind size: 22KD<br>Observed bind size: 50KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-alpha-picoband-trade-antibody-pb9246-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9246-1_1-WB-anti-tnf-alpha-picoband-antibody.jpgAnti-TNF alpha Antibody Picoband&reg;Anti-TNF alpha Picoband antibody&#44; PB9246&#44; Western blotting<br>All lanes: Anti TNF alpha (PB9246) at 0.5ug/ml<br>WB: Recombinant Mouse TNF alpha Protein 0.5ng<br>Predicted bind size: 15KD<br>Observed bind size: 15KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9246-2_1-WB-anti-tnf-alpha-picoband-antibody.jpgAnti-TNF alpha Antibody Picoband&reg;Anti-TNF alpha Picoband antibody&#44; PB9246&#44; Western blotting<br>All lanes: Anti TNF alpha (PB9246) at 0.5ug/ml<br>Lane 1: Mouse Kidney Tissue Lysate at 50ug<br>Lane 2: Mouse Intestine Tissue Lysate at 50ug<br>Predicted bind size: 25KD<br>Observed bind size: 25KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9246-fphar-12-680139-g003.jpgAnti-TNF alpha Antibody Picoband&reg;Effect of gallic acid on the expression of TACE and TNF-α. (A). The expression of TACE in serum was detected by enzyme-linked immunosorbent assay (F (4, 10) = 89.68, p < 0.001). (B). Detection of the TACE and β -actin protein expression in the DRG by Western blotting. (C). The relative protein expression of TACE (F (4, 10) = 24.12, p < 0.001). (D). The expression of TNF-α mRNA was detected by qRT-PCR using β -actin as the housekeeper gene (F (4, 10) = 174.2, p < 0.001). (E). The expression of TNF-α mRNA was detected by qRT-PCR using GAPDH as the housekeeper gene (F (4, 10) = 22.63, p < 0.001). (F). In the DRG, TNF-α and β -actin protein expression was detected by Western blotting. (G) . The relative protein expression of TNF-α (F (4, 10) = 33.47, p < 0.001). (H). The expression of TNF-α in serum was detected by enzyme-linked immunosorbent assay (F (4, 10) = 212.2, p < 0.001). One-way ANOVA was used to detect the expression of TACE and TNF-α. Each group consisted of eight rats. Data are presented as mean ± SEM. *** p < 0.001 versus the Sham group, ### p < 0.001 versus the CCI group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.680139/full'>34512324</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9246-fphar-09-00331-g007.jpgAnti-TNF alpha Antibody Picoband&reg;The synthesized GILZ-p inhibited LPS induced inflammatory cytokine expression in Müller cells. Western blot analysis was performed to determine the protein expression levels of pro-IL-1β (A,B) , MCP-1 (C,D) , TNF-α (E,F) , and ICAM-1 (G,H) in Müller cells treated with 1000 ng/ml LPS in combination with different concentrations of GILZ-p (0.01, 0.1, 1, and 10 μM)for 24 h. β-actin was used as the loading control. The results of quantitative analysis, as determined by densitometric analysis, were expressed as relative to β-actin. Data represent the mean ± SE; the Mann–Whitney U -test was used for comparisons between two groups. n = 3 for each group. ∗ P < 0.05, ∗∗ P < 0.01. TNF-α, tumor necrosis factor-alpha; ICAM-1, intercellular adhesion molecule-1; MCP-1, monocyte chemoattractant protein-1.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00331/full'>29681857</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9246-fphar-09-00331-g008.jpgAnti-TNF alpha Antibody Picoband&reg;The synthesized GILZ-p decreased LPS induced inflammatory cytokine secretion in culture medium of Müller cells. The Enzyme-Linked Immunosorbent Assays (ELISA) were performed to determine the protein expression levels of IL-1β, MCP-1, TNF-α, and ICAM-1 in Müller cells treated with 1000 ng/ml LPS in combination with different concentrations of GILZ-p (0.01, 0.1, 1, and 10 μM) for 24 h. Data represent the mean ± SE; the Mann–Whitney U -test was used for comparisons between two groups. n = 6 for each group. ∗ P < 0.05, ∗∗ P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00331/full'>29681857</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calretinin-picoband-trade-antibody-pb9248-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9248-1-WB-anti-calretinin-picoband-antibody.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg;Anti-Calretinin Picoband antibody&#44; PB9248&#44; Western blotting<br>All lanes: Anti Calretinin (PB9248) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 31KD<br>Observed bind size: 38KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9248-2-IHC-anti-calretinin-picoband-antibody.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg;Anti-Calretinin Picoband antibody&#44; PB9248&#44; IHC(P)<br>IHC(P): Mouse Brain Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9248-3-IHC-anti-calretinin-picoband-antibody.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg;Anti-Calretinin Picoband antibody&#44; PB9248&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9248-4-IHC-anti-calretinin-picoband-antibody.jpgAnti-Calretinin/CALB2 Antibody Picoband&reg;Anti-Calretinin Picoband antibody&#44; PB9248&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd8-alpha-picoband-trade-antibody-pb9249-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9249-cd8a-primary-antibodies-if-testing-2.jpgAnti-CD8 alpha/CD8A Antibody Picoband&reg; IHC analysis of CD8 alpha using anti-CD8 alpha antibody (PB9249). <br> CD8 alpha was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD8 alpha Antibody (PB9249) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd63-picoband-trade-antibody-pb9250-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9250-10020_2025_1192_fig6_html.pngAnti-CD63 Antibody Picoband&reg;Identification of hAMSC-Exos ( A ) The morphology of hAMSC-Exos was observed using transmission electron microscopy. Scale bar = 100 nm. ( B ) The hAMSC-Exos particle size distribution was detected using NTA. ( C ) Western blot was used to detect the expression of hAMSC-Exos surface proteins CD9, CD63 and CD81 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-025-01192-8'>40329179</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9250-cd63-primary-antibodies-wb-testing-1.jpgAnti-CD63 Antibody Picoband&reg; Western blot analysis of CD63 using anti-CD63 antibody (PB9250). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MDA-MB-453 whole cell lysates,<br> Lane 2: human U87 whole cell lysates,<br> Lane 3: human HL-60 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD63 antigen affinity purified polyclonal antibody (Catalog # PB9250) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD63 at approximately 30-60 kDa. The expected band size for CD63 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9250-cd63-primary-antibodies-ihc-testing-2.jpgAnti-CD63 Antibody Picoband&reg; IHC analysis of CD63 using anti-CD63 antibody (PB9250). <br> CD63 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD63 Antibody (PB9250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9250-cd63-primary-antibodies-ihc-testing-3.jpgAnti-CD63 Antibody Picoband&reg; IHC analysis of CD63 using anti-CD63 antibody (PB9250). <br> CD63 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD63 Antibody (PB9250) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd86-b7-2-picoband-trade-antibody-pb9251-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9251-cd86-primary-antibodies-wb-testing-1.jpgAnti-CD86 Antibody Picoband&reg; Western blot analysis of CD86 using anti-CD86 antibody (PB9251). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human Daudi whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD86 antigen affinity purified polyclonal antibody (Catalog # PB9251) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD86 at approximately 60-80 kDa. The expected band size for CD86 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9251-cd86-primary-antibodies-wb-testing-2.jpgAnti-CD86 Antibody Picoband&reg; Western blot analysis of CD86 using anti-CD86 antibody (PB9251). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates,<br> Lane 2: mouse thymus tissue lysates,<br> Lane 3: mouse J774A.1 whole cell lysates,<br> Lane 4: mouse Ana-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD86 antigen affinity purified polyclonal antibody (Catalog # PB9251) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD86 at approximately 60-80 kDa. The expected band size for CD86 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9251-cd86-primary-antibodies-ihc-testing-3.jpgAnti-CD86 Antibody Picoband&reg; IHC analysis of CD86 using anti-CD86 antibody (PB9251). <br> CD86 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD86 Antibody (PB9251) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fas-picoband-trade-antibody-pb9252-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9252-1-WB-anti-fas-cd95-antibody.jpgAnti-Fas Antibody Picoband&reg;Anti-FAS Picoband antibody&#44; PB9252&#44; Western blotting<br>All lanes: Anti FAS (PB9252) at 0.5ug/ml<br>WB: Recombinant Human FAS Protein 0.5ng<br>Predicted bind size: 35KD<br>Observed bind size: 35KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9252-2-WB-anti-fas-cd95-antibody.jpgAnti-Fas Antibody Picoband&reg;Anti-FAS Picoband antibody&#44; PB9252&#44; Western blotting<br>All lanes: Anti FAS (PB9252) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Lane 3: A549 Whole Cell Lysate at 40ug<br>Lane 4: SMMC Whole Cell Lysate at 40ug<br>Lane 5: K562 Whole Cell Lysate at 40ug<br>Predicted bind size: 37KD<br>Observed bind size: 37KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9252-3-IHC-anti-fas-cd95-antibody.jpgAnti-Fas Antibody Picoband&reg;Anti-FAS Picoband antibody&#44; PB9252&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hif-1-alpha-picoband-trade-antibody-pb9253-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-hif1a-primary-antibodies-wb-testing-1.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg; Western blot analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Untreated human HepG2 whole cell lysates,<br> Lane 2: Cobalt Chloride treated human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF 1 alpha antigen affinity purified polyclonal antibody (PB9253) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HIF 1 alpha at approximately 120 kDa. The expected band size for HIF 1 alpha is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-fmed-11-1477685-g001.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Histological and immunohistochemical images of the rat livers of the control group and hypoxia group of CLD. (A) H&E staining images (× 40) of the control and hypoxia group of CLD. (B) Masson staining images (× 40) of the control group and hypoxia group of CLD. (C) Pimonidazole Immunohistochemical staining images (× 40) of control and hypoxia group of CLD. (D) HIF-1α Immunohistochemical staining images (× 40) of control and hypoxia group of CLD. (E) α-SMA Immunohistochemical staining images (× 40) of control and hypoxia group of CLD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2024.1477685/full'>39906347</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-fmed-11-1477685-g004.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Correlations for the hypoxia score (HIF-1α) with the pseudo-diffusion coefficient (D*) and the T1 mapping. (A) HIF-1α correlated positively with the D* ( r = 0.556, p = 0.020). (B) HIF-1α correlated positively with T1 mapping ( r = 0.505, p = 0.039).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/medicine/articles/10.3389/fmed.2024.1477685/full'>39906347</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-13020_2024_1039_fig5_html.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;ZXGD inhibited hypoxia, oxidative stress and inflammation in rats with PH and PASMCs induced with PDGF-BB. A – E Expression of HIF-1α, Nrf2, IL-1β, IL-6 and IL-10 in lung tissue of rats were detected using Western blot (n = 3 or 6). F – I Concentration of IL-6, IL-1β, TNF-α and IL-10 in lung tissue of rats were examined by ELISA (n = 6). J Level of ROS in PASMCs was assessed with flow cytometry, and statistical data was obtained. ** p < 0.01, *** p < 0.001 vs control, # p < 0.05, ## p < 0.01, ### p < 0.001 vs PH or PDGF-BB<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11657759/'>39696593</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-13020_2024_1039_fig7_html.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;ZXGD modulated HIF-1α mediated pulmonary vascular remodeling. A Expression level of HIF-1α in lung tissue of rats was tested with PCR (n = 3). B Expression level of HIF-1α in PASMCs transfected with siRNA (n = 6). C Effects of ZXGD on viability of PASMCs transfected with HIF-1α siRNA was examined by MTT (n = 6). D – G Levels of IL-6, IL-10, LDL-C and decadienyl- l -carnitine in PASMCs transfected with HIF-1α siRNA. H – K Expression level of HIF-1α, Caspase-3, PCNA, PLIN2 in PASMCs transfected with HIF-1α siRNA (n ≥ 3). * p < 0.05, *** p < 0.001 vs control, # p < 0.05, ## p < 0.01, ### p < 0.001 vs PH or PDGF-BB<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11657759/'>39696593</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-13020_2024_1039_fig8_html.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Effect of neohesperidin and naringin on cell viability, HIF-1α, Caspase3, PLIN2 in PASMCs. A – D Effects of neohesperidin and naringin on PASMCs viability were observed by MTT (n = 6). E – J Expression levels of HIF-1α, Caspase3, PLIN2 in PASMCs were evaluated by Western Blot (n ≥ 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs control, # p < 0.05, ## p < 0.01, ### p < 0.001 vs PDGF-BB. 5 represents 5 µM, 10 represents 10 µM<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11657759/'>39696593</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-wjgo-15-464-g005.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Effects of Xiaojianzhong decoction on gastric mucosal hypoxia in N-methyl-N’-nitro-N-nitrosoguanidine-induced gastric precancerous lesions rats. A and B: Western blot analysis was performed to detect hypoxia-inducible factor 1α (HIF-1α), E1B19000 interacting protein 3 (Bnip-3) protein expression ( n = 3); C and D: HIF-1α, Bnip-3 mRNA expression was determined using real-time polymerase chain reaction analysis ( n = 3); E-G: Hypoxia-induced autophagy-related protein HIF-1α, Bnip-3 expression was determined using immunofluorescence ( n = 5); HIF-1α, Bnip-3 proteins positive score was determined using Image J. Data are expressed as mean ± SD. a P < 0.05, b P < 0.01, c P < 0.001 vs control group; d P < 0.05, e P < 0.01, f P < 0.001 vs model group. XJZ-L: Low dose of Xiaojianzhong decoction; XJZ-M: Middle dose of Xiaojianzhong decoction; XJZ-H: High dose of Xiaojianzhong decoction; HIF-1α: Hypoxia-inducible factor 1α; Bnip-3: B cell lymphoma/Leukemia-2 and adenovirus E1B19000 interacting protein 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10052669/&orig_host=www.ncbi.nlm.nih.gov'>37009319</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-wjgo-15-464-g006.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Effects of Xiaojianzhong decoction on hypoxia-induced glycolysis in gastric mucosal epithelial cells. A-C: Hypoxia-induced glycolysis-related protein hypoxia-inducible factor 1α (HIF-1α), CD147 expression was determined using immunofluorescence ( n = 5); HIF-1α, CD147 proteins positive score was determined using Image J; D-F: CD147, monocarboxylate transporter (MCT1), and MCT4 mRNA expression was determined using real-time polymerase chain reaction analysis ( n = 3); G and H: Sirtuin 6 (SIRT6) expression was determined using immunohistochemistry ( n = 5); SIRT-6 positive score was determined using Image J. Data are expressed as mean ± SD. a P < 0.05, b P < 0.01, c P < 0.001 vs control group; d P < 0.05, e P < 0.01, f P < 0.001 vs model group. XJZ-L: Low dose of Xiaojianzhong decoction; XJZ-M: Middle dose of Xiaojianzhong decoction; XJZ-H: High dose of Xiaojianzhong decoction; HIF-1α: Hypoxia-inducible factor 1α; MCT: Monocarborxylat transporter.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10052669/&orig_host=www.ncbi.nlm.nih.gov'>37009319</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-fphys-13-925752-g006.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Response of ZF4 cells to hypoxic stress. (A) Cell Viability was analyzed with PrestoBlue™ HS Cell Viability Regent under normoxia or hypoxia treated for 1, 2, 3 and 4 d. (B) The mRNA expression of iron absorption and storage gene in ZFL cells was quantified by real-time RT–PCR under normoxia and hypoxia for 3 days. (C) Western blot analysis of Hif-1α and Ferritin expression in ZF4 cells cultured under normoxia and hypoxia for 3 days. (D) The microscope analysis of morphology changes in ZF4 cells under normoxia or hypoxia treated with or without FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM) for 3 days. (E) Cell Viability was analyzed with PrestoBlue™ HS Cell Viability Regent under normoxia or hypoxia treated with or without FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM). (F–H) Analysis of changes in general ROS (F) , mitochondrial-derived ROS (G) and lipid peroxidation (H) levels in cells with H2DCFDA, MitoSOX and C11-BODIPY probe under normoxia and hypoxia for 3 days. Cells under hypoxic stress were rescued with FAC (2.5 mM), DFO (10 µM) and Fer-1 (2.5 µM). Normoxia was used as a control group for significance analysis. Error bars, mean ± s.d., n = 3 (biological replicates).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.925752/full?utm_source=dlvr.it&utm_medium=twitter'>36091397</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-thnov10p12011g002.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;AhR significantly suppressed IRF1 and HIF-1α expression in a murine CaOx nephrocalcinosis model. (A) RNA-seq heatmap showing significantly altered mRNAs in SGA-treated BMDMs. (B) Volcano plots showing mRNA transcripts that were differentially expressed between LPS-treated and SGA-treated BMDMs. Significantly downregulated and upregulated mRNAs are shown in green and red, respectively, whereas genes that were not significantly changed are shown in black. (C) IHC staining for AhR, IRF1, and HIF-1α in the kidneys of FICZ-treated mice with CaOx nephrocalcinosis (200×; scale bar: 20 µm). (D) qRT-PCR was used to assess AhR, IRF1, and HIF-1α expression in kidney samples from FICZ-treated mice (n = 6) with CaOx nephrocalcinosis compared to kidney samples from model mice. (E, F) Pearson's correlation coefficient analysis (n = 30) of the expression levels of AhR and IRF1 (E) or HIF-1α (F). Each dot represents an individual animal. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (D).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-thnov10p12011g003.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;AhR suppressed IRF1 and HIF-1α to attenuate CaOx crystal-stimulated M1 macrophage polarization in vitro . (A) BMDMs and COM-treated TECs co-culture model. (B, C) Western blotting analysis was used to detect AhR, HIF-1α, IRF1, NF-κB p65, iNOS, and Arg-1 expression after FICZ treatment and the upregulation or downregulation of AhR in BMDMs. β-actin served as a normalization control. (D, E) iNOS (M1 macrophage marker, green) and Arg-1 (M2 macrophage marker, red) distribution in BMDMs were detected by immunofluorescence (200×; scale bar: 20 µm). (F, G) qRT-PCR analysis of iNOS, IL-6, CIITA, Arg-1, Chi3l3 and Fizz1 expression to further determine polarization state of BMDM. The data are shown as the means ± SD of triplicate experiments. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (F, G).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-thnov10p12011g004.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;AhR transcriptionally activates miR-142a to inhibit IRF1 and HIF-1α expression. (A) The top 30 miRNAs in BMDMs that are regulated by LPS are arranged in a miRNA array heatmap. In addition, miRNAs predicted to be under the transcriptional control of AhR (according to analysis with the JASPAR database) are noted. (B) Venn diagram analyses were performed to identify miRNAs that can both target IRF1 and HIF-1α and that are under the transcriptional control of AhR. (C) Renal expression of mmu-miR-142a-3p in mice (n = 6) with CaOx nephrocalcinosis following treatment with an AhR neutralizing antibody or FICZ treatment was assessed via FISH (200×; scale bar: 20 µm). (D) qRT-PCR was performed to measure mmu-miR-142a-3p expression in BMDMs using U6 RNA as a normalization control. (E, F) ChIP assays and ChIP qPCR analysis showed that AhR bound to the miR-142a promoter in BMDMs treated with the AhR overexpression plasmid. (G) A schematic model showed that AhR directly binds to the miR-142a promoter and activates its transcription. (H, I) WT and mutated miR-142a targeting sequences in the IRF1 and HIF-1α 3'-UTR regions that were used to construct luciferase reporters, with reporters bearing these IRF1 (J) or HIF-1α (L) 3'-UTR sequences co-transfected along with miR-142a mimic (100 nM). IRF1 (K) and HIF-1α (M) mRNA levels were detected via qRT-PCR in BMDMs following miR-142a mimic or inhibitor transfection. Western blotting (N, O) analysis enabled the detection of IRF1 and HIF-1α expression while also assessing the levels of iNOS and Arg-1 to monitor the polarization state of BMDMs following miR-142a mimic or inhibitor transfection. β-actin was employed as a normalization control. The data are shown as the means ± SD of triplicate experiments. *P < 0.05; **P < 0.01, as assessed via Student's t test (D, F) or one-way ANOVA (J-M, O).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-thnov10p12011g005.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;AhR activation in vitro decrease M1 macrophage polarization to inhibit kidney inflammation and injury through the AhR-miR-142a-IRF1/HIF-1α axis in vitro . (A) Western blotting analysis enabled the detection of AhR, HIF-1α, IRF1, NF-κB p65, iNOS, and Arg-1 expression in BMDMs. β-actin was detected as an internal control. (B) iNOS (M1 macrophage marker, green) and Arg-1 (M2 macrophage marker, red) distributions in BMDMs were detected by immunofluorescence (200×; scale bar: 20 µm). (C) Schematic diagram of BMDMs phagocytic capacity testing. (D) Fluorescence microscopy was performed to analyse the phagocytic ability of BMDMs (200×; scale bar: 20 µm). (E) qRT-PCR analysis of iNOS, IL-6, CIITA, Arg-1, Chi3l3 and Fizz1 expression to further determine polarization state of BMDM. (F) ELISA was used to quantify cytokine levels in the co-culture media. The data are shown as the means ± SD of triplicate experiments. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (E, F).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-thnov10p12011g006.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;AhR activation suppressed the deposition of CaOx crystal and CaOx nephrocalcinosis-mediated kidney inflammation and injury through the AhR-miR-142a-IRF1/HIF-1α axis in vivo . (A) Experimental overview. (B) The deposition of renal CaOx crystal in FICZ- and/or antagomiR-142a-treated mice was assessed via polarized light optical microscopy (20×; scale bar: 500 µm). Pizzolato staining was employed as a means of detecting these CaOx crystal in corticomedullary tissue, while PAS was utilized to evaluate injury to TECs (200×; scale bar: 20 µm), and TUNEL staining was employed to assess renal TECs death (200×; scale bar: 50 µm). (C) PET-CT scanning was employed as a means of assessing renal inflammation state in CaOx nephrocalcinosis mice. (D) IHC was used to analyse AhR, IRF1, and HIF-1α expression, and FISH was used to detect miR-142a expression in renal tissue (200×; scale bar: 20 µm). (E) iNOS (M1 macrophage marker, red) and Arg-1 (M2 macrophage marker, green) distributions in renal tissues were detected by immunofluorescence (200×; scale bar: 50 µm). (F) On days 3 and 10, the serum pro-inflammatory IL-1β, TNF-α, and IL-6 levels and the anti-inflammatory IL-10 levels were measured by ELISA. n = 6 per group. *P < 0.05; **P < 0.01, as assessed via one-way ANOVA (F).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7667681/&orig_host=www.ncbi.nlm.nih.gov'>33204326</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-oncotarget-08-22406-g002.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Tumors from CD47-deficient mice exhibited improved tumor angiogenesis and vascular integrity compared to those from WT mice. Tumors were surgically removed from WT or CD47 −/− mice 11 days after tumor cell injection for the following analyses ( n = 4 per group). ( A ) Representative images of CD31 staining (black arrow) of tumor sections (Scale bar, 20 μm). ( B ) The microvessel density (MVD) quantified by counting positive cells in six randomly selected fields (400×) using Image Pro Plus 6.0 software. ( C ) CD31 mRNA levels in tumors quantified by real-time qPCR. ( D ) Representative images of HIF-1A staining (pink) of tumor sections (Scale bar, 20 μm). ( E ) Percentages of HIF-1A + cells in tumors quantified by counting positive cells in six randomly selected fields (400×) using Image Pro Plus 6.0 software. ( F ) Representative images of CD144 staining (red) of tumor sections (Scale bar, 20 μm). ( G ) Percentages of CD144 + area in tumors quantified using Image Pro Plus 6.0 software. Data are mean ± SDs. ** P < 0.01, *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5410232/&orig_host=www.ncbi.nlm.nih.gov'>27283989</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253_41065_2025_499_fig10_html.pngAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg; Effects of XLW on HIF1α and PTGS2 expressions in rat extraocular muscle tissues. ( A ) The immunohistochemical slice of HIF1α, ( B ) HIF1α immunohistochemical quantitative analysis, ( C ) WB of PTGS2 levels, and ( D ) quantification of the WB in PTGS2. ** p < 0.01, **** p < 0.0001 versus NC group; # p < 0.05, ## p < 0.01 versus HT group; n = 6 or 8 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s41065-025-00499-0'>40696458</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-qims-15-01-662-f3.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;HIF-1α expression was analyzed by immunohistochemistry. Results of a 61-year-old male patient who underwent NAC and surgery treatment for PDAC, and had a CAP score of 2, which indicated that he was a responder. HIF-1α was highly expressed in the (A) nucleus (weighted score 12), (B) cytoplasm (weighted score 12), and (C) stroma (weighted score 4); results of a 30-year-old male patient who underwent NAC and surgery treatment for PDAC, and had a CAP score of 3, which indicated that he was a non-responder. HIF-1α was lowly expressed in the (D) nucleus (weighted score 4), (E) cytoplasm (weighted score 4), and (F) stroma (weighted score 4). 40×; scale bar =20 µm. NAC, neoadjuvant chemotherapy; PDAC, pancreatic ductal adenocarcinoma; CAP, the College of American Pathologists; HIF-1α, hypoxia-inducible factor-1-alpha. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11744163/'>39839013</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-qims-15-01-662-f4.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Receiver operator characteristic curves of nuclear HIF-1α expression (A), %ΔCA19-9 (B), and tumor differentiation (C) for predicting the post-NAC pathological response. HIF-1α, hypoxia-inducible factor-1α; NAC, neoadjuvant chemotherapy; %Δ, [(post-NAC − pre-NAC)/pre-NAC]; CA19-9, carbohydrate antigen 19-9; AUC, area under the curve; CI, confidence interval. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11744163/'>39839013</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-qims-15-01-662-f5.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Comparison of CT values between the nuclear HIF-1α-high and -low expression PDAC patients following NAC. ***, P<0.001; **, P=0.003. CT, computed tomography; HIF-1α, hypoxia-inducible factor-1-alpha; PDAC, pancreatic ductal adenocarcinoma; NAC, neoadjuvant chemotherapy; AP, arterial phase; DP, delayed phase; HU, c. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11744163/'>39839013</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-41467_2025_63749_fig6_html.pngAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;In vivo therapeutic efficacy of photosynthetic system in myocardial ischemia mice. A Schematic illustration of the animal treatment protocol in the MI model. B ECG of mice before (left) and after (right) induction. C Echocardiography of mice in different treatment groups after treatment for 7 days. D – G Quantitative analysis of the EF, FS, LVESV and LVESD at different periods ( n = 6, n represent biologically independent animal samples in each group, mean ± s.d.). H Representative images of the mouse ischemic cardiac detected by Color Doppler Flow Imaging. I TTC staining of hearts from mice in different groups. J Masson trichrome staining displayed the fibrous tissue (blue) and myocardium (red) and HE trichrome staining of sections of hearts from mice in different groups, scale bar: 100 μm. K HIF-1α (red) and DAPI immunostaining (blue) of myocardium in different groups, scale bar: 50 μm. L – N Quantitative analysis of collagen area, fibrosis area, infarct area ( n = 6, n represent biologically independent animal samples in each group, mean ± s.d.). Statistical analysis was performed using unpaired two-tailed Student’s t test. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-63749-9'>41028003</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-41467_2025_63749_fig4_html.pngAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Validation of the alleviating hypoxia capacity and anti-apoptotic effects of photosynthetic system on cardiomyocytes. A Bright-field and fluorescence-field images of C. pyre in HAMA, scale bar, 5 μm. B Activity of C. pyre . C Dissolved O 2 level changes of the solution containing HCU with various treatments. D Flow chart of induced hypoxia in cardiomyocytes. E Fluorescence images of H9c2 cells staining with RDPP probe after various treatments. F Fluorescence images of H9c2 cells staining with HIF-1α antibody (red) and DAPI (blue) after various treatments, scale bar: 50 μm. G Quantitative fluorescence analysis of RDPP probes ( n = 5, n represent the number of independent experiments, mean ± s.d.). H Quantitative fluorescence analysis of HIF-1α ( n = 5, n represent the number of independent experiments, mean ± s.d.). I The levels of proteins including HIF-1α, Bcl-2, C-Caspase 3 and Cyt c in H9c2 cells analyzed by western blotting. J – M Corresponding quantitative histograms of protein expression ( n = 5, n represent the number of independent experiments, mean ± s.d.). N Anti-apoptosis analysis of H9c2 cells by flow cytometry after various treatments. O Corresponding quantitative histograms of anti-apoptosis rate ( n = 3, n represent the number of independent experiments, mean ± s.d.). Statistical analysis was performed using unpaired two-tailed Student’s t test. Independent experiments were performed ( n = 3) for ( A ) with similar results. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-63749-9'>41028003</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-fgene-16-1646991-g007.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg;Construction of animal models and analysis of pathological damage. (A) TTC staining plots of the Sham and VD group. (B) Motion trajectory diagram. (C) Representative images stained with HE (scale bars = 50 μm). (D) Representative histopathological images obtained from TUNEL staining (scale bars = 50 μm), showing changes in the cortex hippocampus CA1. (E) Quantification of TUNEL+ cells in the hippocampal CA1 (n = 3). (F) Expression profile of CD31 between Sham and VD groups (qPCR). **P < 0.01. (G) Expression profile of HIF-1α between Sham and VD groups (qPCR; n = 6). ***P < 0.001. (H) WB strips. (I) Expression profile of CD31 between Sham and VD groups (WB). *P < 0.05. (J) Expression profile of HIF-1α between Sham and VD groups (WB; n = 3). ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1646991/full'>40988927</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9253-3-IHC-anti-hif-1-alpha-picoband-antibody.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg; IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). <br> HIF 1 alpha was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9253-4-IHC-anti-hif-1-alpha-picoband-antibody.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg; IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). <br> HIF 1 alpha was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9253-5-IHC-anti-hif-1-alpha-picoband-antibody_1.jpgAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg; IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253). <br> HIF 1 alpha was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9253-hif1a-primary-antibodies-wb-testing-2.pngAnti-HIF-1-alpha/HIF1A Antibody Picoband&reg; Western blot analysis of HIF1A using anti-HIF1A antibody (PB9253).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: mouse 4T1 whole cell lysates,<br> Lane 2: LPS-stimulated mouse 4T1 whole cell lysates,<br> Lane 3: Low-dose drug mouse 4T1 whole cell lysates,<br> Lane 4: Medium-dose drug mouse 4T1 whole cell lysates,<br> Lane 5: High-dose drug mouse 4T1 whole cell lysates,<br> Lane 6: Positive control drug mouse 4T1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1 hour at RT. The membrane was incubated with rabbit anti-HIF1A antigen affinity purified monoclonal antibody (Catalog # PB9253) at 1:2500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with ChemiDoc MP system. A specific band was detected for HIF1A at approximately 110-120 kDa. The expected band size for HIF1A is at 120kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-alpha-5-picoband-trade-antibody-pb9254-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9254-itga5-primary-antibodies-wb-testing-1.jpgAnti-Integrin alpha 5/ITGA5 Antibody Picoband&reg; Western blot analysis of ITGA5 using anti-ITGA5 antibody (PB9254). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA5 antigen affinity purified polyclonal antibody (Catalog # PB9254) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA5 at approximately 130 kDa. The expected band size for ITGA5 is at 115 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kcnd1-picoband-trade-antibody-pb9256-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9256-1-WB-anti-kcnd1-kv4-1-picoband-antibody.jpgAnti-KCND1 Antibody Picoband&reg;Anti-KCND1 Picoband antibody&#44; PB9256&#44; Western blotting<br>All lanes: Anti KCND1 (PB9256) at 0.5ug/ml<br>WB: Recombinant Human KCND1 Protein 0.5ng<br>Predicted bind size: 39KD<br>Observed bind size: 39KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9256-2-WB-anti-kcnd1-kv4-1-picoband-antibody.jpgAnti-KCND1 Antibody Picoband&reg;Anti-KCND1 Picoband antibody&#44; PB9256&#44; Western blotting<br>All lanes: Anti KCND1 (PB9256) at 0.5ug/ml<br>Lane 1: Mouse Brain Tissue Lysate at 50ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: COLO320 Whole Cell Lysate at 40ug<br>Lane 4: A549 Whole Cell Lysate at 40ug<br>Predicted bind size: 71KD<br>Observed bind size: 71KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-kit-picoband-trade-antibody-pb9258-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9258-1-WB-anti-kit-scfr-antibody.jpgAnti-c-Kit Antibody Picoband&reg; Western blot analysis of C-Kit using anti-C-Kit antibody (PB9258). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Recombinan Human C-Kit Protein 0.5ng <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody (Catalog # PB9258) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Kit at approximately 49KD. The expected band size for C-Kit is at 49KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9258-fonc-12-859275-g002.jpgAnti-c-Kit Antibody Picoband&reg;Lumiflavin treatment reduces the development of DDP resistance of ovarian cancer OVCAR-3 cells line that is associated with cancer stem cells (CSCs). DDP resistance of ovarian cancer OVCAR-3 cell lines were induced by gradient concentration increment method ( ), and treated with 10, 20, 40, and 80 μm lumiflavin intervention simultaneously. The cell inhibition rate and resistance index of OVCAR-3cells to DDP were detected by CCK-8 assay kit. The proportion of CSCs (CD133+/CD177+ double positive cells) in OVCAR-3 cells was detected by flow cytometry. (A) Inhibitory curves of lumiflavin and DDP on OVCAR-3 and OVCAR-3/DDP cells. (B) Resistance index of OVCAR-3/DDP cells compared with OVCAR-3 cells. (C) Flow detection results of CD133+/CD117+ cells ratio of OVCAR-3 and OVCAR-3/DDP cells. (D) Statistical analysis graph of (C) . Mean ± SD ( n = 3). ** P < 0.01 difference between groups; * P < 0.05 difference between groups. DDP, cisplatin; OVCAR-3/DDP, DDP resistant OVCAR-3 cell lines; LUM, lumiflavin.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2022.859275/full'>35669418</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9258-fonc-12-859275-g004.jpgAnti-c-Kit Antibody Picoband&reg;Effects of lumiflavin treatment on phenotypic differentiation of DDP-resistant cancer stem cells (CSCs/DDP). CSCs, CSCs/DDP were treated with 80 μM DDP, and CSCs/DDP were treated with 80 μM DDP and 20, 40, 80 μM lumiflavin for 72 h. Co-expression of CD133+/CD117+, CD44+/CD177, and CD44+/CD24– of CSCs/DDP were detected through flow cytometry. (A) Flow detection results of CD133+/CD117+, CD44+/CD177, and CD44+/CD24- cells of CSCs/DDP. (B) Statistical analysis graph of (A) . ** P < 0.01 between groups. Mean ± SD ( n = 3). CSCs/DDP, CSCs from DDP resistant OVCAR-3 cell lines; LUM, lumiflavin.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2022.859275/full'>35669418</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9258-2-WB-anti-kit-scfr-antibody.jpgAnti-c-Kit Antibody Picoband&reg; Western blot analysis of C-Kit using anti-C-Kit antibody (PB9258). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: HEPG2 Whole Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C-Kit antigen affinity purified polyclonal antibody (Catalog # PB9258) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C-Kit at approximately 109KD. The expected band size for C-Kit is at 109KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9258-3-IHC-anti-kit-scfr-antibody.jpgAnti-c-Kit Antibody Picoband&reg; IHC analysis of C-Kit using anti-C-Kit antibody (PB9258).<br> C-Kit was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C-Kit Antibody (PB9258) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9258-c-kit-primary-antibodies-fc-testing-4.pngAnti-c-Kit Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-C-Kit antibody (PB9258).<br>Overlay histogram showing K562 cells stained with PB9258 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Kit Antibody (PB9258&#44;1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psa-picoband-trade-antibody-pb9259-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9259-klk3-primary-antibodies-wb-testing-1.jpgAnti-Prostate Specific Antigen/KLK3 Antibody Picoband&reg; Western blot analysis of KLK3 using anti-KLK3 antibody (PB9259). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human LNCAP whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLK3 antigen affinity purified polyclonal antibody (Catalog # PB9259) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLK3 at approximately 29 kDa. The expected band size for KLK3 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9259-klk3-primary-antibodies-ihc-testing-2.jpgAnti-Prostate Specific Antigen/KLK3 Antibody Picoband&reg; IHC analysis of KLK3 using anti-KLK3 antibody (PB9259). <br> KLK3 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KLK3 Antibody (PB9259) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-4-picoband-trade-antibody-pb9260-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9260-1-WB-anti-kallikrein-4-picoband-antibody.jpgAnti-Kallikrein 4/KLK4 Antibody Picoband&reg;Anti-Picoband antibody&#44; PB&#44; Western blotting<br>All lanes: Anti Kallikrein 4 (PB9260) at 0.5ug/ml<br>WB: Recombinant Human Kallikrein 4 Protein 0.5ng<br>Predicted bind size: 25KD<br>Observed bind size: 25KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9260-2-WB-anti-kallikrein-4-picoband-antibody.jpgAnti-Kallikrein 4/KLK4 Antibody Picoband&reg;Anti-Picoband antibody&#44; PB&#44; Western blotting<br>All lanes: Anti Kallikrein 4 (PB9260) at 0.5ug/ml<br>Lane 1: 293T Whole Cell Lysate at 40ug<br>Lane 2: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 27KD<br>Observed bind size: 27KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm7-picoband-trade-antibody-pb9261-boster.html2026-03-24T05:04:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-mcm7-primary-antibodies-wb-testing-1.jpgAnti-MCM7 Antibody Picoband&reg; Western blot analysis of MCM7 using anti-MCM7 antibody (PB9261). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM7 antigen affinity purified polyclonal antibody (Catalog # PB9261) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM7 at approximately 81 kDa. The expected band size for MCM7 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-2-ihc-anti-mcm7-picoband-antibody.jpgAnti-MCM7 Antibody Picoband&reg; IHC analysis of MCM7 using anti-MCM7 antibody (PB9261). <br> MCM7 was detected in a paraffin-embedded section of Mouse Testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCM7 Antibody (PB9261) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-3-ihc-anti-mcm7-picoband-antibody.jpgAnti-MCM7 Antibody Picoband&reg; IHC analysis of MCM7 using anti-MCM7 antibody (PB9261). <br> MCM7 was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCM7 Antibody (PB9261) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-4-ihc-anti-mcm7-picoband-antibody.jpgAnti-MCM7 Antibody Picoband&reg; IHC analysis of MCM7 using anti-MCM7 antibody (PB9261). <br> MCM7 was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCM7 Antibody (PB9261) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-5-if-anti-mcm7-picoband-antibody.jpgAnti-MCM7 Antibody Picoband&reg; IHC analysis of MCM7 using anti-MCM7 antibody (PB9261).<br> MCM7 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-MCM7 Antibody (PB9261) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-9_1.pngAnti-MCM7 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-MCM7 antibody (PB9261). <br> Overlay histogram showing A431 cells stained with PB9261 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM7 Antibody (PB9261&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-6_1.jpgAnti-MCM7 Antibody Picoband&reg; IF analysis of MCM7 using anti-MCM7 antibody (PB9261) <br> MCM7 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MCM7 Antibody (PB9261) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-7_1.jpgAnti-MCM7 Antibody Picoband&reg; IF analysis of MCM7 using anti-MCM7 antibody (PB9261).<br> MCM7 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-MCM7 Antibody (PB9261) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9261-8_1.jpgAnti-MCM7 Antibody Picoband&reg; IF analysis of MCM7 using anti-MCM7 antibody (PB9261).<br> MCM7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MCM7 Antibody (PB9261) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mad1-picoband-trade-antibody-pb9262-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-1_1.jpgAnti-MAD1/MAD1L1 Antibody Picoband&reg; Western blot analysis of MAD1 using anti-MAD1 antibody (PB9262). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44; <br> Lane 2: human Raji whole cell lysates&#44; <br> Lane 3: human Jurkat whole cell lysates&#44; <br> Lane 4: human HepG2 whole cell lysates. <br> Lane 5: human HL-60 whole cell lysates. <br> Lane 6: human THP-1 whole cell lysates. <br> Lane 7: human Caco-2 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody (Catalog # PB9262) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-2_1.jpgAnti-MAD1/MAD1L1 Antibody Picoband&reg; Western blot analysis of MAD1 using anti-MAD1 antibody (PB9262). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat kidney tissue lysates&#44; <br> Lane 3: rat lung tissue lysates&#44; <br> Lane 4: rat liver tissue lysates&#44; <br> Lane 5: mouse brain tissue lysates&#44; <br> Lane 6: mouse kidney tissue lysates&#44; <br> Lane 7: mouse lung tissue lysates&#44; <br> Lane 8: mouse liver tissue lysates&#44; <br> Lane 9: mouse NIH3T3 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAD1 antigen affinity purified polyclonal antibody (Catalog # PB9262) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAD1 at approximately 83KD. The expected band size for MAD1 is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-3_1.jpgAnti-MAD1/MAD1L1 Antibody Picoband&reg; IHC analysis of MAD1 using anti-MAD1 antibody (PB9262). <br> MAD1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAD1 Antibody (PB9262) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-4.jpgAnti-MAD1/MAD1L1 Antibody Picoband&reg; IHC analysis of MAD1 using anti-MAD1 antibody (PB9262). <br> MAD1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAD1 Antibody (PB9262) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-5.jpgAnti-MAD1/MAD1L1 Antibody Picoband&reg; IHC analysis of MAD1 using anti-MAD1 antibody (PB9262). <br> MAD1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAD1 Antibody (PB9262) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-6.jpgAnti-MAD1/MAD1L1 Antibody Picoband&reg; IHC analysis of MAD1 using anti-MAD1 antibody (PB9262).<br> MAD1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-MAD1 Antibody (PB9262) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-7.pngAnti-MAD1/MAD1L1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-MAD1 antibody (PB9262). <br> Overlay histogram showing A431 cells stained with PB9262 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAD1 Antibody (PB9262&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9262-8.jpgAnti-MAD1/MAD1L1 Antibody Picoband&reg; IF analysis of MAD1 using anti-MAD1 antibody (PB9262). <br> MAD1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MAD1 Antibody (PB9262) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-menin-picoband-trade-antibody-pb9263-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9263-1-WB-anti-menin-picoband-antibody.jpgAnti-Menin/MEN1 Antibody Picoband&reg;Anti-Menin Picoband antibody&#44; PB9263&#44; Western blotting<br>All lanes: Anti Menin (PB9263) at 0.5ug/ml<br>WB: Recombinant Human Menin Protein 0.5ng<br>Predicted bind size: 39KD<br>Observed bind size: 39KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9263-2-WB-anti-menin-picoband-antibody.jpgAnti-Menin/MEN1 Antibody Picoband&reg;Anti-Menin Picoband antibody&#44; PB9263&#44; Western blotting<br>All lanes: Anti Menin (PB9263) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: 293T Whole Cell Lysate at 40ug<br>Lane 3: SMMC Whole Cell Lysate at 40ug<br>Lane 4: HEPA Whole Cell Lysate at 40ug<br>Lane 5: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 68KD<br>Observed bind size: 68KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mitofusin-1-picoband-trade-antibody-pb9264-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9264-mitofusin-1-primary-antibodies-wb-testing-1.jpgAnti-Mitofusin 1/MFN1 Antibody Picoband&reg; Western blot analysis of Mitofusin 1 using anti-Mitofusin 1 antibody (PB9264). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: rat heart tissue lysates, <br> Lane 3: mouse kidney tissue lysates, <br> Lane 4: mouse heart tissue lysates,<br> Lane 5: human placenta tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 1 antigen affinity purified polyclonal antibody (Catalog # PB9264) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 1 at approximately 84KD. The expected band size for Mitofusin 1 is at 84KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mitofusin-2-picoband-trade-antibody-pb9265-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9265-1-WB-anti-mitofusin-2-picoband-antibody.jpgAnti-Mitofusin 2/MFN2 Antibody Picoband&reg; Western blot analysis of Mitofusin-2 using anti-Mitofusin-2 antibody (PB9265). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Recombinant Human Mitofusin-2 Protein 0.5ng. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin-2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin-2 at approximately 45KD. The expected band size for Mitofusin-2 is at 45KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9265-nrr-15-2143-g006.jpgAnti-Mitofusin 2/MFN2 Antibody Picoband&reg;ε-Viniferin drives the expression of mitochondrial homeostasis-related proteins. Western blot assays demonstrated that ε-viniferin treatment increases the expression levels of mitochondrial biosynthesis-related proteins (PGC-1α and TFAM); mitochondrial fusion and fission proteins (DRP1, MFN2, and FIS1), and mitophagy-related proteins (BNIP3, NIX, and Parkin), and decreases the expression levels of P62. Data are shown as the mean ± SD. # P < 0.05, vs . ε-viniferin treatment group (one-way analysis of variance followed by Bonferroni test). 1: Model group; 2: ε-viniferin treatment group; 3: ε-viniferin + SIRT3 shRNA group; 4: ε-viniferin + FOXO3 shRNA group; 5: ε-viniferin + control shRNA group. VFN: ε-Viniferin.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7716051/&orig_host=www.ncbi.nlm.nih.gov'>32394973</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9265-2-WB-anti-mitofusin-2-picoband-antibody.jpgAnti-Mitofusin 2/MFN2 Antibody Picoband&reg; Western blot analysis of Mitofusin-2 using anti-Mitofusin-2 antibody (PB9265). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: HELA Whole Cell Lysate&#44;<br> Lane 2: A549 Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin-2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin-2 at approximately 86KD. The expected band size for Mitofusin-2 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9265-3_1.jpgAnti-Mitofusin 2/MFN2 Antibody Picoband&reg; Western blot analysis of Mitofusin 2 using anti-Mitofusin 2 antibody (PB9265). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat heart tissue lysates&#44; <br> Lane 3: rat kidney tissue lysates&#44;<br> Lane 4: mouse brain tissue lysates&#44;<br> Lane 5: mouse heart tissue lysates&#44;<br> Lane 6: mouse kidney tissue lysates&#44;<br> Lane 7: mouse small intestine tissue lysates&#44;<br> Lane 8: mouse NIH3T3 whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 2 antigen affinity purified polyclonal antibody (Catalog # PB9265) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 2 at approximately 86KD. The expected band size for Mitofusin 2 is at 86KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mgmt-picoband-trade-antibody-pb9266-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9266-mgmt-primary-antibodies-wb-testing-1_1.jpgAnti-MGMT Antibody Picoband&reg; Western blot analysis of MGMT using anti-MGMT antibody (PB9266). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human CACO-2 whole cell lysates, <br> Lane 2: human Raji whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGMT antigen affinity purified polyclonal antibody (Catalog # PB9266) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGMT at approximately 22 kDa. The expected band size for MGMT is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9266-mgmt-primary-antibodies-ihc-testing-2.jpgAnti-MGMT Antibody Picoband&reg; IHC analysis of MGMT using anti-MGMT antibody (PB9266). <br> MGMT was detected in a paraffin-embedded section of human colorectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MGMT Antibody (PB9266) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9266-3_1.jpgAnti-MGMT Antibody Picoband&reg; ICC analysis of MGMT using anti-MGMT antibody (PB9266).<br> MGMT was detected in immunocytochemical section of human MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-MGMT Antibody (PB9266) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9266-mgmt-primary-antibodies-if-testing-4.jpgAnti-MGMT Antibody Picoband&reg; IF analysis of MGMT using anti- MGMT antibody (PB9266). <br> MGMT was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- MGMT Antibody (PB9266) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9266-5.jpgAnti-MGMT Antibody Picoband&reg; IF analysis of MGMT using anti-MGMT antibody (PB9266). <br> MGMT was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MGMT Antibody (PB9266) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9266-6.jpgAnti-MGMT Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-MGMT antibody (PB9266).<br> Overlay histogram showing THP-1 cells stained with PB9266 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MGMT Antibody (PB9266,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp3-picoband-trade-antibody-pb9267-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9267-1-WB-anti-mmp-3-antibody.jpgAnti-MMP3 Antibody Picoband&reg;Anti-MMP3 Picoband antibody&#44; PB9267&#44; Western blotting<br>All lanes: Anti MMP3 (PB9267) at 0.5ug/ml<br>WB: Recombinant Human MMP3 Protein 0.5ng<br>Predicted band size: 36KD<br>Observed band size: 36KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9267-2-WB-anti-mmp-3-antibody.jpgAnti-MMP3 Antibody Picoband&reg;Anti-MMP3 Picoband antibody&#44; PB9267&#44; Western blotting<br>All lanes: Anti MMP3 (PB9267) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: U20S Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Lane 4: PANC Whole Cell Lysate at 40ug<br>Lane 5: COLO320 Whole Cell Lysate at 40ug<br>Predicted band size: 54KD<br>Observed band size: 54KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9267-fonc-10-602670-g004.jpgAnti-MMP3 Antibody Picoband&reg;Identification of miR-18a target genes in cisplatin-resistant ovarian cancer. (A) qRT-PCR was performed with total RNA extracted from miR-18a-OMM or CNT-OMM transfected A2780CP20 cells. Rq (relative expression) values were calculated relative to CNT-OMM samples. (B) Western blot analysis was performed with protein extracts (50 µg) of OMM transfected cells. (C) Densitometric analysis of band intensities was performed and relative values were calculated using the intensity of β-actin as control (**P < 0.01). (D) Dual - luciferase assay was performed to assess the binding of miR-18a to the 3’UTR region of MMP-3. Experiments were performed in triplicates. Mean ± SEM is shown (*P < 0.05). (E) Western blot analysis was conducted for MMP-3 with protein extracts of ovarian cancer cell lines.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.602670/full'>33392094</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9267-fonc-10-602670-g005.jpgAnti-MMP3 Antibody Picoband&reg;Effect of MMP-3 silencing on cell viability, cell growth and invasion. MMP-3 silencing in (A) A2780CP20 and (B) OVCAR3CIS was assessed by Western blot analysis of cells collected 24 h after transfection with siRNAs. Cell viability was assessed in (C) A2780CP20 and (D) OVCAR3CIS with AlamarBlue dye 72 h after siRNA transfection (*P < 0.05, **P < 0.01). Colony formation assay was performed after siRNA transfection in (E) A2780CP20 and (F) OVCAR3CIS. % of clonogenicity was calculated relative to CNT-siRNA-transfected cells (*P < 0.05). Invasion assay was performed after siRNA (25 nM siRNA, final concentration) transfection in (G) A2780CP20 and (H) OVCAR3CIS cells. Untransfected cells were taken as 100% invasion. Images of invaded cells were taken with a light microscope on 20X magnification lenses. Mean ± SEM is shown as a result of three independent experiments (*P < 0.05, ****P < 0.0001).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.602670/full'>33392094</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-msk1-picoband-trade-antibody-pb9268-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9268-msk1-primary-antibodies-wb-testing-1.jpgAnti-MSK1/RPS6KA5 Antibody Picoband&reg; Western blot analysis of MSK1 using anti-MSK1 antibody (PB9268). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSK1 antigen affinity purified polyclonal antibody (Catalog # PB9268) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSK1 at approximately 90 kDa. The expected band size for MSK1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9268-2-ihc-anti-msk1-picoband-antibody.jpgAnti-MSK1/RPS6KA5 Antibody Picoband&reg; IHC analysis of MSK1 using anti-MSK1 antibody (PB9268). <br> MSK1 was detected in a paraffin-embedded section of Mouse Cardiac Muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MSK1 Antibody (PB9268) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9268-3-ihc-anti-msk1-picoband-antibody.jpgAnti-MSK1/RPS6KA5 Antibody Picoband&reg; IHC analysis of MSK1 using anti-MSK1 antibody (PB9268). <br> MSK1 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MSK1 Antibody (PB9268) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9268-4-ihc-anti-msk1-picoband-antibody.jpgAnti-MSK1/RPS6KA5 Antibody Picoband&reg; IHC analysis of MSK1 using anti-MSK1 antibody (PB9268). <br> MSK1 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MSK1 Antibody (PB9268) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9268-msk1-primary-antibodies-if-testing-5.jpgAnti-MSK1/RPS6KA5 Antibody Picoband&reg; IF analysis of MSK1 using anti-MSK1 antibody (PB9268). <br> MSK1 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MSK1 Antibody (PB9268) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lkb1-picoband-trade-antibody-pb9270-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9270-stk11-primary-antibodies-wb-testing-1.jpgAnti-LKB1/STK11 Antibody Picoband&reg;Western blot analysis of LKB1/STK11 using anti-LKB1/STK11 antibody (PB9270). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LKB1/STK11 antigen affinity purified polyclonal antibody (PB9270) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LKB1/STK11 at approximately 55 kDa. The expected band size for LKB1/STK11 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpc5-picoband-trade-antibody-pb9271-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9271-1-WB-anti-trpc5-picoband-antibody.jpgAnti-TRPC5 Antibody Picoband&reg;Anti-TRPC5 Picoband antibody&#44; PB9271&#44; Western blotting<br>All lanes: Anti TRPC5 (PB9271) at 0.5ug/ml<br>WB: Recombinant Human TRPC5 Protein 0.5ng<br>Predicted bind size: 50KD<br>Observed bind size: 50KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9271-2-WB-anti-trpc5-picoband-antibody.jpgAnti-TRPC5 Antibody Picoband&reg;Anti-TRPC5 Picoband antibody&#44; PB9271&#44; Western blotting<br>All lanes: Anti TRPC5 (PB9271) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: U87 Whole Cell Lysate at 40ug<br>Lane 3: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 111KD<br>Observed bind size: 111KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9271-trpc5-primary-antibodies-wb-testing-3.jpgAnti-TRPC5 Antibody Picoband&reg; Western blot analysis of TRPC5 using anti-TRPC5 antibody (PB9271). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC5 antibody (PB9271) at 1:200 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using a fluorescent. A specific band was detected for TRPC5 at approximately 111 kDa. The expected band size for TRPC5 is at 111 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trp-7-picoband-trade-antibody-pb9272-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9272-1-WB-anti-trp-7-picoband-antibody.jpgAnti-TRP 7/TRPC7 Antibody Picoband&reg;Anti-TRP 7 Picoband antibody&#44; PB9272&#44; Western blotting<br>All lanes: Anti TRP 7 (PB9272) at 0.5ug/ml<br>WB: Recombinant Human TRP 7 Protein 0.5ng<br>Predicted bind size: 39KD<br>Observed bind size: 39KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9272-2_1-WB-anti-trp-7-picoband-antibody.jpgAnti-TRP 7/TRPC7 Antibody Picoband&reg;Anti-TRP 7 Picoband antibody&#44; PB9272&#44; Western blotting<br>All lanes: Anti TRP 7 (PB9272) at 0.5ug/ml<br>Lane 1: Mouse Brain Tissue Lysate at 50ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Lane 3: COLO320 Whole Cell Lysate at 40ug<br>Lane 4: SKOV Whole Cell Lysate at 40ug<br>Predicted bind size: 99KD<br>Observed bind size: 99KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vwf-picoband-trade-antibody-pb9273-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9273-1-WB-anti-vwf-von-willebrand-factor-picoband-antibody.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;Anti-VWF Picoband antibody&#44; PB9273&#44; Western blotting<br>All lanes: Anti VWF (PB9273) at 0.5ug/ml<br>WB: Recombinant Mouse VWF Protein 0.5ng<br>Predicted bind size: 37KD<br>Observed bind size: 37KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9273-2-WB-anti-vwf-von-willebrand-factor-picoband-antibody.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;Anti-VWF Picoband antibody&#44; PB9273&#44; Western blotting<br>All lanes: Anti VWF (PB9273) at 0.5ug/ml<br>WB: Mouse Lung Tissue Lysate at 50ug<br>Predicted bind size: 309KD<br>Observed bind size: 309KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9273-3-IHC-anti-vwf-von-willebrand-factor-picoband-antibody.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;Anti-VWF Picoband antibody&#44; PB9273&#44; IHC(P)<br>IHC(P): Mouse Liver Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-13020_2024_926_fig10_html.pngAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;Representative images showed IHC staining of HIF-1α, VEGF, and vWF in the cerebral cortex (magnification 400 ×) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-024-00926-w'>38600597</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-13287_2021_2280_fig4_html.pngAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;The detection of ovarian angiogenesis in different treatment groups at day 28 after transplantation. a Histochemical images displayed vWF- and CD34-positive cells in ovarian stroma from different treatment groups. Scale bar 50 μm and 25 μm. b MVD quantification determined by CD34- and vWF-positive cells showed the number of microvessels in ovarian sections of the different groups. * p < 0.05 vs. Bu/Cy group; ## p < 0.01, #### p < 0.0001 vs. SA-BG group; $$$ p < 0.0005, $$$$ p < 0.0001 vs. SA-BG/hAECs group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-021-02280-2'>33794993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-13287_2021_2260_fig5_html.pngAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;Effects of hAECs on angiogenesis in the injured uteruses. a IHC staining of von Willebrand factor (vWF) reflected the blood vessel density in the uterine scars of different groups at day 30 and 60 after injection of hAECs or PBS ( n = 8 uterine horns per group). Scale bars = 25 μm. b IHC staining of VEGFA in the uterine scars at day 30 and 60 after injection of hAECs or PBS in different groups ( n = 8 uterine horns per group). Scale bars = 25 μm. c Statistical analysis of the blood vessel density indicated by vWF-positive staining. d The VEGFA expression level was semi-qualified by calculating the percentage of positive cells per field under a magnification of × 400. e Western blot analysis showed that VEGFA expression of the uterine scars in the hAECs group and PBS group at day 5 after injection of hAECs or PBS. f The grayscale values of the western blots were analyzed. The protein level of VEGFA was normalized to that of β-tubulin ( n = 5) (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; NS, P ≥ 0.05) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-021-02260-6'>33762002</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-13287_2019_1368_fig4_html.pngAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;hAECs facilitated endometrial recovery in the IUA model. A IHC staining of vWF reflected the MVD of the endometrium. The microvessels, which were vWF-positive, are indicated by arrows in the figure. MVD was reduced in the IUA group and increased in the hAEC-treated group. B IHC staining showed that the expression of VEGF was higher in the hAEC-treated group than in the IUA group. C The expression of PCNA decreased in the IUA group and reached almost normal levels in the hAEC-treated group. a–c, scale bar = 100 μm; d–f, scale bar = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-019-1368-9'>31412924</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-13287_2019_1368_fig5_html.pngAnti-Von Willebrand Factor/VWF Antibody Picoband&reg;hAECs facilitated endometrial recovery in the IUA model. A According to IHC staining, the number of ER-positive cells was higher in the hAEC-treated group than in the IUA group. B There was no difference in PR expression among these three groups. C VEGF expression was semi-quantified, and the number of positive cells per field was calculated. D MVD was valued by counting microvascular vessels, which were vWF-positive. E – G . PCNA, ER, and PR expression levels were semi-quantified by calculating the percentage of positive cells per field (* p < 0.05; ** p < 0.01; *** p < 0.001; NS, p ≥ 0.05). a–c, scale bar = 100 μm; d–f, scale bar = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13287-019-1368-9'>31412924</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-4.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg; IHC analysis of VWF using anti-VWF antibody (PB9273). <br> VWF was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VWF Antibody (PB9273) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-vwf-primary-antibodies-if-testing-5.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg; IF analysis of VWF using anti-VWF antibody (PB9273). <br> VWF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-VWF Antibody (PB9273) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9273-vwf-primary-antibodies-if-testing-6.jpgAnti-Von Willebrand Factor/VWF Antibody Picoband&reg; IF analysis of VWF and alpha-Smooth Muscle Actin using anti-VWF antibody (PB9273) and anti-alpha-Smooth Muscle Actin antibody (MA1106).<br> VWF and alpha-Smooth Muscle Actin a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-VWF antibody (PB9273) and mouse anti-alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-3-antibody-rp1046-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1046-1-WB-anti-il-3-antibody.jpgAnti-Interleukin-3 IL3 Antibody Picoband&reg;Anti-IL-3 antibody&#44; RP1046&#44; Western blotting<br>All lanes: Anti IL-3 (RP1046) at 0.5ug/ml<br>WB: Recombinant Rat IL-3 Protein 0.5ng<br>Predicted bind size: 15KD<br>Observed bind size: 15KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1046-2-WB-anti-il-3-antibody.jpgAnti-Interleukin-3 IL3 Antibody Picoband&reg;Anti-IL-3 antibody&#44; RP1046&#44; Western blotting<br>All lanes: Anti IL-3 (RP1046) at 0.5ug/ml<br>Lane 1: PC-12 Whole Cell Lysate at 40ug<br>Lane 2: NRK Whole Cell Lysate at 40ug<br>Lane 3: RH35 Whole Cell Lysate at 40ug<br>Predicted bind size: 17KD<br>Observed bind size: 37KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-13-antibody-rp1047-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-Interleukin-13 IL13 Antibody Picoband&reg;Boster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-13-antibody-rp1048-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1048-il13-primary-antibodies-elisa-testing-1.jpgAnti-Interleukin-13 IL13 Antibody Sandwich ELISA - Recombinant rat IL13 protein standard curve.<br> Use in combination with reagents from Rat IL13 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0900). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mbd2-antibody-rp1049-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1049-1-WB-anti-mbd2-antibody.jpgAnti-MBD2 Antibody Picoband&reg;Anti-MBD2 antibody&#44; RP1049&#44; Western blotting<br>All lanes: Anti MBD2 (RP1049) at 0.5ug/m<br>lWB: Recombinant Human MBD2 Protein 0.5ng<br>Predicted bind size: 40KD<br>Observed bind size: 40KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1049-2-WB-anti-mbd2-antibody.jpgAnti-MBD2 Antibody Picoband&reg;Anti-MBD2 antibody&#44; RP1049&#44; Western blotting<br>All lanes: Anti MBD2 (RP1049) at 0.5ug/ml<br>Lane 1: SGC Whole Cell Lysate at 40ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Lane 4: K562 Whole Cell Lysate at 40ug<br>Predicted bind size: 47KD<br>Observed bind size: 47KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd146-antibody-rp1050-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1050-cd146-primary-antibodies-wb-testing-1.jpgAnti-CD146/MCAM Antibody Picoband&reg; Western blot analysis of CD146 using anti-CD146 antibody (RP1050). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U2OS whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD146 antigen affinity purified polyclonal antibody (Catalog # RP1050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD146 at approximately 120 kDa. The expected band size for CD146 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1050-3-IHC-anti-cd146-antibody.jpgAnti-CD146/MCAM Antibody Picoband&reg;Anti-CD146 antibody&#44; RP1050&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcb4-picoband-trade-antibody-pb9275-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9275-abcb4-primary-antibodies-ihc-testing-3.jpgAnti-ABCB4 Antibody Picoband&reg;IHC analysis of ABCB4 using anti-ABCB4 antibody (PB9275). <br>ABCB4 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB4 Antibody (PB9275) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9275-abcb4-primary-antibodies-fcm-testing-3.jpgAnti-ABCB4 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-ABCB4 antibody (PB9275). <br>Overlay histogram showing HepG2 cells stained with PB9275 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB4 Antibody (PB9275, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9275-abcb4-primary-antibodies-wb-testing-1.jpgAnti-ABCB4 Antibody Picoband&reg; Western blot analysis of ABCB4 using anti-ABCB4 antibody (PB9275). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: rat RH35 whole cell lysates, <br> Lane 4: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody (Catalog # PB9275) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 150 kDa. The expected band size for ABCB4 is at 142 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9275-abcb4-primary-antibodies-ihc-testing-2_1.jpgAnti-ABCB4 Antibody Picoband&reg; IHC analysis of ABCB4 using anti-ABCB4 antibody (PB9275). <br> ABCB4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCB4 Antibody (PB9275) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-midkine-antibody-rp1051-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1051-mdk-primary-antibodies-wb-testing-1.jpgAnti-Midkine/MDK Antibody Picoband&reg; Western blot analysis of MDK using anti-MDK antibody (RP1051). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human U20S whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDK antigen affinity purified polyclonal antibody (Catalog # RP1051) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDK at approximately 16 kDa. The expected band size for MDK is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-survivin-antibody-rp1052-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1052-survivin-primary-antibodies-wb-testing-1.jpgAnti-Survivin/BIRC5 Antibody Picoband&reg; Western blot analysis of Survivin using anti-Survivin antibody (RP1052). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse thymus tissue lysates, <br> Lane 2: mouse SP2/0 whole cell lysates, <br> Lane 3: mouse RAW264.7 whole cell lysates, <br> Lane 4: mouse ANA-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody (Catalog # RP1052) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Survivin at approximately 16 kDa. The expected band size for Survivin is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1052-survivin-primary-antibodies-ihc-testing-2.jpgAnti-Survivin/BIRC5 Antibody Picoband&reg; IHC analysis of Survivin using anti-Survivin antibody (RP1052). <br> Survivin was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin Antibody (RP1052) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1052-survivin-primary-antibodies-ihc-testing-3.jpgAnti-Survivin/BIRC5 Antibody Picoband&reg; IHC analysis of Survivin using anti-Survivin antibody (RP1052). <br> Survivin was detected in a paraffin-embedded section of mouse lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin Antibody (RP1052) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1052-survivin-primary-antibodies-fcm-testing-4.jpgAnti-Survivin/BIRC5 Antibody Picoband&reg; Flow Cytometry analysis of Neuro2a cells using anti-Survivin antibody (RP1052). <br>Overlay histogram showing Neuro2a cells stained with RP1052 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Survivin Antibody (RP1052, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serca1-atpase-antibody-rp1053-boster.html2026-03-24T05:04:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1053-atp2a1-primary-antibodies-wb-testing-1.jpgAnti-SERCA1 ATPase/ATP2A1 Antibody Picoband&reg;Western blot analysis of ATP2A1 using anti-ATP2A1 antibody (RP1053). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue lysates,<br> Lane 2: mouse skeletal muscle tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A1 antigen affinity purified polyclonal antibody (Catalog # RP1053) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ATP2A1 at approximately 110 kDa. The expected band size for ATP2A1 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1053-atp2a1-primary-antibodies-ihc-testing-1.jpgAnti-SERCA1 ATPase/ATP2A1 Antibody Picoband&reg;IHC analysis of ATP2A1 using anti-ATP2A1 antibody (RP1053). <br> ATP2A1 was detected in a paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP2A1 Antibody (RP1053) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1053-atp2a1-primary-antibodies-ihc-testing-2.jpgAnti-SERCA1 ATPase/ATP2A1 Antibody Picoband&reg;IHC analysis of ATP2A1 using anti-ATP2A1 antibody (RP1053). <br> ATP2A1 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP2A1 Antibody (RP1053) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1053-atp2a1-primary-antibodies-fcm-testing-1.jpgAnti-SERCA1 ATPase/ATP2A1 Antibody Picoband&reg;Flow Cytometry analysis of 293T cells using anti-ATP2A1 antibody (RP1053). <br> Overlay histogram showing 293T cells stained with RP1053 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP2A1 Antibody (RP1053, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serca2-atpase-antibody-rp1054-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1054-atp2a2-primary-antibodies-wb-testing-1.jpgAnti-SERCA2 ATPase/ATP2A2 Antibody Picoband&reg; Western blot analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 antigen affinity purified polyclonal antibody (Catalog # RP1054) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP2A2 at approximately 115 kDa. The expected band size for ATP2A2 is at 115 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1054-atp2a2-primary-antibodies-ihc-testing-4.jpgAnti-SERCA2 ATPase/ATP2A2 Antibody Picoband&reg;IHC analysis of SERCA2/ATP2A2 using anti-SERCA2/ATP2A2 antibody (RP1054). <br>SERCA2/ATP2A2 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERCA2/ATP2A2 Antibody (RP1054) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1054-2-IHC-anti-serca2-atpase-antibody.jpgAnti-SERCA2 ATPase/ATP2A2 Antibody Picoband&reg; IHC analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054). <br> ATP2A2 was detected in a paraffin-embedded section of Mouse Lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ATP2A2 Antibody (RP1054) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1054-3_1-IHC-anti-serca2-atpase-antibody.jpgAnti-SERCA2 ATPase/ATP2A2 Antibody Picoband&reg; IHC analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054). <br> ATP2A2 was detected in a paraffin-embedded section of Rat Lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ATP2A2 Antibody (RP1054) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atp2a3-antibody-rp1055-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1055-1-WB-anti-atp2a3-serca3-atpase-antibody.jpgAnti-SERCA3 ATPase/ATP2A3 Antibody Picoband&reg;Anti-ATP2A3 antibody&#44; RP1055&#44; Western blotting<br>All lanes: Anti ATP2A3 (RP1055) at 0.5ug/ml<br>Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug<br>Predicted bind size: 114KD<br>Observed bind size: 114KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1055-2-IHC-anti-atp2a3-serca3-atpase-antibody.jpgAnti-SERCA3 ATPase/ATP2A3 Antibody Picoband&reg;Anti-ATP2A3 antibody&#44; RP1055&#44; IHC(P)<br>IHC(P): Mouse Thymus Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1055-3-IHC-anti-atp2a3-serca3-atpase-antibody.jpgAnti-SERCA3 ATPase/ATP2A3 Antibody Picoband&reg;Anti-ATP2A3 antibody&#44; RP1055&#44;IHC(P)<br> IHC(P): Rat Thymus Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1055-atp2a3-primary-antibodies-ihc-testing-4.jpgAnti-SERCA3 ATPase/ATP2A3 Antibody Picoband&reg;IHC analysis of SERCA3/ATP2A3 using anti-SERCA3/ATP2A3 antibody (RP1055). <br>SERCA3/ATP2A3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERCA3/ATP2A3 Antibody (RP1055) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1055-atp2a3-primary-antibodies-ihc-testing-5.jpgAnti-SERCA3 ATPase/ATP2A3 Antibody Picoband&reg;IHC analysis of SERCA3/ATP2A3 using anti-SERCA3/ATP2A3 antibody (RP1055). <br>SERCA3/ATP2A3 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SERCA3/ATP2A3 Antibody (RP1055) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kat13a-src1-antibody-rp1056-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1056-1_1.jpgAnti-KAT13A/SRC1/NCOA1 Antibody Picoband&reg; Western blot analysis of KAT13A/SRC1 using anti-KAT13A/SRC1 antibody (RP1056). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: SK-OV-3 whole cell lysates, <br> Lane 2: SGC-7901 whole cell lysates, <br> Lane 3: Jurkat whole cell lysates, <br> Lane 4: rat kidney tissue lysates, <br> Lane 5: NIH/3T3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT13A/SRC1 antigen affinity purified polyclonal antibody (Catalog # RP1056) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT13A/SRC1 at approximately 157KD. The expected band size for KAT13A/SRC1 is at 157KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1056-2.jpgAnti-KAT13A/SRC1/NCOA1 Antibody Picoband&reg; IF analysis of KAT13A/SRC1 using anti-KAT13A/SRC1 antibody (RP1056). <br> KAT13A/SRC1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-KAT13A/SRC1 Antibody (RP1056) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1056-3.jpgAnti-KAT13A/SRC1/NCOA1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-KAT13A/SRC1 antibody (RP1056).<br>Overlay histogram showing SiHa cells stained with RP1056 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KAT13A/SRC1 Antibody (RP1056,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mif-picoband-trade-antibody-pb9274-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9274-mif-primary-antibodies-wb-testing-1.jpgAnti-MIF Antibody Picoband&reg; Western blot analysis of MIF using anti-MIF antibody (PB9274). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (Catalog # PB9274) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9274-mif-primary-antibodies-ihc-testing-2.jpgAnti-MIF Antibody Picoband&reg; IHC analysis of MIF using anti-MIF antibody (PB9274). <br> MIF was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIF Antibody (PB9274) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9274-mif-primary-antibodies-ihc-testing-3.jpgAnti-MIF Antibody Picoband&reg; IHC analysis of MIF using anti-MIF antibody (PB9274). <br> MIF was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIF Antibody (PB9274) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9274-mif-primary-antibodies-if-testing-4.jpgAnti-MIF Antibody Picoband&reg; IF analysis of MIF using anti-MIF antibody (PB9274). <br> MIF was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MIF Antibody (PB9274) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9274-mif-primary-antibodies-fcm-testing-5.jpgAnti-MIF Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-MIF antibody (PB9274). <br> Overlay histogram showing Jurkat cells stained with PB9274 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIF Antibody (PB9274, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-occludin-antibody-rp1057-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1057-1-WB-anti-occludin-antibody.jpgAnti-Occludin/OCLN Antibody Picoband&reg;Anti-Occludin antibody&#44; RP1057&#44; Western blotting<br>All lanes: Anti Occludin (RP1057) at 0.5ug/ml<br>Lane 1: SW620 Whole Cell Lysate at 40ug<br>Lane 2: COLO320 Whole Cell Lysate at 40ug<br>Lane 3: A549 Whole Cell Lysate at 40ug<br>Lane 4: 293T Whole Cell Lysate at 40ug Lane 5: U87 Whole Cell Lysate at 40ug<br>Predicted bind size: 59KD<br>Observed bind size: 59KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1057-2-IHC-anti-occludin-antibody.jpgAnti-Occludin/OCLN Antibody Picoband&reg;Anti-Occludin antibody&#44; RP1057&#44;IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-delta-antibody-rp1058-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1058-prkcd-primary-antibodies-wb-testing-1.jpgAnti-PKC delta/PRKCD Antibody Picoband&reg;Western blot analysis of PRKCD using anti-PRKCD antibody (RP1058). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human CACO-2 whole cell lysates,<br> Lane 4: mouse small intestine tissue lysates.<br> Lane 5: mouse ovary tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCD antigen affinity purified polyclonal antibody (Catalog # RP1058) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKCD at approximately 78 kDa. The expected band size for PRKCD is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1058-prkcd-primary-antibodies-wb-testing-2.jpgAnti-PKC delta/PRKCD Antibody Picoband&reg;Western blot analysis of PRKCD using anti-PRKCD antibody (RP1058). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat lung tissue lysates,<br> Lane 3: mouse brain tissue lysates,<br> Lane 4: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKCD antigen affinity purified polyclonal antibody (Catalog # RP1058) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRKCD at approximately 78 kDa. The expected band size for PRKCD is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1058-prkcd-primary-antibodies-ihc-testing-1.jpgAnti-PKC delta/PRKCD Antibody Picoband&reg;IHC analysis of PRKCD using anti-PRKCD antibody (RP1058). <br> PRKCD was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKCD Antibody (RP1058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1058-prkcd-primary-antibodies-ihc-testing-2.jpgAnti-PKC delta/PRKCD Antibody Picoband&reg;IHC analysis of PRKCD using anti-PRKCD antibody (RP1058). <br> PRKCD was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PRKCD Antibody (RP1058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-shbg-picoband-trade-antibody-pb9326-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9326-1-WB-anti-shbg-picoband-antibody.jpgAnti-Sex Hormone Binding Globulin/SHBG Antibody Picoband&reg;Anti-SHBG Picoband antibody&#44; PB9326&#44; Western blotting<br> All lanes: Anti SHBG (PB9326) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 44KD<br>Observed bind size: 70KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp47-picoband-trade-antibody-pb9325-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9325-serpinh1-primary-antibodies-wb-testing-1_1.jpgAnti-Hsp47/SERPINH1 Antibody Picoband&reg; Western blot analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human SK-N-SH whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: rat lung tissue lysates, <br> Lane 5: rat kidney tissue lysates, <br> Lane 6: rat L6 whole cell lysates, <br> Lane 7: mouse lung tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp47/SERPINH1 antigen affinity purified polyclonal antibody (Catalog # PB9325) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp47/SERPINH1 at approximately 46 kDa. The expected band size for Hsp47/SERPINH1 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9325-serpinh1-primary-antibodies-ihc-testing-2.jpgAnti-Hsp47/SERPINH1 Antibody Picoband&reg; IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). <br> Hsp47/SERPINH1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsp47/SERPINH1 Antibody (PB9325) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9325-serpinh1-primary-antibodies-ihc-testing-3.jpgAnti-Hsp47/SERPINH1 Antibody Picoband&reg; IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). <br> Hsp47/SERPINH1 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsp47/SERPINH1 Antibody (PB9325) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9325-serpinh1-primary-antibodies-ihc-testing-4.jpgAnti-Hsp47/SERPINH1 Antibody Picoband&reg; IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). <br> Hsp47/SERPINH1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsp47/SERPINH1 Antibody (PB9325) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9325-serpinh1-primary-antibodies-ihc-testing-5.jpgAnti-Hsp47/SERPINH1 Antibody Picoband&reg; IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). <br> Hsp47/SERPINH1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsp47/SERPINH1 Antibody (PB9325) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9325-serpinh1-primary-antibodies-ihc-testing-6.jpgAnti-Hsp47/SERPINH1 Antibody Picoband&reg; IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). <br> Hsp47/SERPINH1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsp47/SERPINH1 Antibody (PB9325) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9325-serpinh1-primary-antibodies-ihc-testing-7.jpgAnti-Hsp47/SERPINH1 Antibody Picoband&reg; IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody (PB9325). <br> Hsp47/SERPINH1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Hsp47/SERPINH1 Antibody (PB9325) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nf-kb-p65-picoband-trade-antibody-pb9324-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9324-fphar-15-1447560-g005.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Molecular docking diagram. (A) HBOA-MAPK (B) HBOA-FoXO, (C) HBOA-AMPK, (D) HBOA-JAK, (E) HBOA-STAT, (F) HBOA-NF-κB, (G) HBOA-PPAR, (H) HBOA-Ras, and (I) HBOA-TLR4. (J) Binding energy score diagram obtained by molecular docking.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1447560/full'>39323644</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9324-fphar-15-1447560-g006.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;HBOA inhibits the TLR4/NF-κB signaling pathway. The protein expressions of (A) TLR4 and MyD88, (B) p-IKKα/β, IKKα/β, p-IκBα, IκBα, p-NF-κBp65, and NF-κBp65, and (C) nucleus NF-κBp65, Lamin B1, and cytosol NF-κBp65 were examined by Western blotting. * P < 0.05, ** P < 0.01 versus the model group; ## P < 0.01 versus the normal group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1447560/full'>39323644</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9324-fphar-15-1447560-g008.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg;Integrated analysis of transcriptomics and metabolomics. (A) Correlation heat map. (B) 9-quadrant map. (C) Bubble plot of the Joint-Pathway analysis of differential metabolites and differential genes. (D) Integration of metabolite and gene relationships in the glycerophospholipid metabolic pathway. (E–I) The contents of Glycerophospholipid metabolism pathway-related metabolites and the expression levels of the target genes.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1447560/full'>39323644</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9324-rela-primary-antibodies-wb-testing-1.jpgAnti-NF-kB p65/RELA Antibody Picoband&reg; Western blot analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PB9324). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse NIH/3T3 whole cell lysates,<br> Lane 2: mouse ANA-1 whole cell lysates.<br> Lane 3: rat testis tissue lysates.<br> Lane 4: rat PC-12 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65/RELA antigen affinity purified polyclonal antibody (Catalog # PB9324) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65/RELA at approximately 65 kDa. The expected band size for NF-kB p65/RELA is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dystrophin-picoband-trade-antibody-pb9276-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9276-1_1.jpgAnti-Dystrophin/DMD Antibody Picoband&reg; Western blot analysis of Dystrophin using anti-Dystrophin antibody (PB9276). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates&#44; <br> Lane 2: human K562 whole cell lysates&#44; <br> Lane 3: mouse HEPA1-6 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dystrophin antigen affinity purified polyclonal antibody (Catalog # PB9276) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dystrophin at approximately 427KD. The expected band size for Dystrophin is at 427KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9276-2-IHC-anti-dystrophin-picoband-antibody.jpgAnti-Dystrophin/DMD Antibody Picoband&reg; IHC analysis of Dystrophin using anti-Dystrophin antibody (PB9276). <br> Dystrophin was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Dystrophin Antibody (PB9276) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9276-3_1-IHC-anti-dystrophin-picoband-antibody.jpgAnti-Dystrophin/DMD Antibody Picoband&reg; IHC analysis of Dystrophin using anti-Dystrophin antibody (PB9276). <br> Dystrophin was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Dystrophin Antibody (PB9276) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9276-4-IHC-anti-dystrophin-picoband-antibody.jpgAnti-Dystrophin/DMD Antibody Picoband&reg; IHC analysis of Dystrophin using anti-Dystrophin antibody (PB9276). <br> Dystrophin was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Dystrophin Antibody (PB9276) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9276-5.pngAnti-Dystrophin/DMD Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Dystrophin antibody (PB9276). <br> Overlay histogram showing HepG2 cells stained with PB9276 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Dystrophin Antibody (PB9276&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rad51-picoband-trade-antibody-pb9323-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9323-rad51-primary-antibodies-wb-testing-2.jpgAnti-Rad51 Antibody Picoband&reg; Western blot analysis of Rad51 using anti-Rad51 antibody (PB9323). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% BSA in tbst for 2 hour at RT. The membrane was incubated with anti-Rad51 antibody (PB9323) at 1:2000 at 4℃ overnight , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a LICOR secondary antibody at a dilution of 1:2000 for 2 hour rotating at RT. The signal is developed using a LICOR Odyssey. A specific band was detected for Rad51 at approximately 36 kDa. The expected band size for Rad51 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9323-rad51-primary-antibodies-wb-testing-1.jpgAnti-Rad51 Antibody Picoband&reg; Western blot analysis of Rad51 using anti-Rad51 antibody (PB9323). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: rat liver tissue lysates, <br> Lane 5: rat C6 whole cell lysates, <br> Lane 6: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51 antigen affinity purified polyclonal antibody (Catalog # PB9323) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51 at approximately 37 kDa. The expected band size for Rad51 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab3a-picoband-trade-antibody-pb9322-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9322-rab3a-primary-antibodies-wb-testing-1.jpgAnti-Rab3A Antibody Picoband&reg; Western blot analysis of Rab3A using anti-Rab3A antibody (PB9322). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab3A antigen affinity purified polyclonal antibody (Catalog # PB9322) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab3A at approximately 25 kDa. The expected band size for Rab3A is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-iota-picoband-trade-antibody-pb9321-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9321-1-WB-anti-pkc-iota-picoband-antibody.jpgAnti-PKC iota/PRKCI Antibody Picoband&reg;Anti-PKC iota Picoband antibody&#44; PB9321&#44; Western blotting<br>All lanes: Anti PKC iota (PB9321) at 0.5ug/ml<br>Lane 1: SHG Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Lane 3: U87 Whole Cell Lysate at 40ug<br>Lane 4: 293T Whole Cell Lysate at 40ug<br>Lane 5: HELA Whole Cell Lysate at 40ug<br>Lane 6: JURKAT Whole Cell Lysate at 40ug<br>Predicted bind size: 68KD<br>Observed bind size: 68KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9321-2-IHC-anti-pkc-iota-picoband-antibody.jpgAnti-PKC iota/PRKCI Antibody Picoband&reg;Anti-PKC iota Picoband antibody&#44; PB9321&#44;IHC(P)<br>IHC(P): Mouse Brain Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9321-3-IHC-anti-pkc-iota-picoband-antibody.jpgAnti-PKC iota/PRKCI Antibody Picoband&reg;Anti-PKC iota Picoband antibody&#44; PB9321&#44;IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9321-4-IHC-anti-pkc-iota-picoband-antibody.jpgAnti-PKC iota/PRKCI Antibody Picoband&reg;Anti-PKC iota Picoband antibody&#44; PB9321&#44;IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hspg2-picoband-trade-antibody-pb9277-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9277-2-IHC-anti-hspg2-perlecan-picoband-antibody.jpgAnti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband&reg;Anti-HSPG2 Picoband antibody&#44; PB9277&#44;IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9277-1_1.jpgAnti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband&reg; Western blot analysis of HSPG2 using anti-HSPG2 antibody (PB9277). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates&#44; <br> Lane 2: human A549 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPG2 antigen affinity purified polyclonal antibody (Catalog # PB9277) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPG2 at approximately 469KD. The expected band size for HSPG2 is at 469KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-epsilon-picoband-trade-antibody-pb9320-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9320-1-WB-anti-pkc-epsilon-picoband-antibody.jpgAnti-PKC epsilon/PRKCE Antibody Picoband&reg;Anti-PKC epsilon Picoband antibody&#44; PB9320&#44; Western blotting<br>All lanes: Anti PKC epsilon (PB9320) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mosue Brain Tissue Lysate at 50ug<br>Lane 3: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 88KD<br>Observed bind size: 88KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9320-2-IHC-anti-pkc-epsilon-picoband-antibody.jpgAnti-PKC epsilon/PRKCE Antibody Picoband&reg;Anti-PKC epsilon Picoband antibody&#44; PB9320&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9320-3-IHC-anti-pkc-epsilon-picoband-antibody.jpgAnti-PKC epsilon/PRKCE Antibody Picoband&reg;Anti-PKC epsilon Picoband antibody&#44; PB9320&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9320-prkce-primary-antibodies-ihc-testing-4.jpgAnti-PKC epsilon/PRKCE Antibody Picoband&reg;IHC analysis of PKC Epsilon/PRKCE using anti-PKC Epsilon/PRKCE antibody (PB9320). <br>PKC Epsilon/PRKCE was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKC Epsilon/PRKCE Antibody (PB9320) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9320-prkce-primary-antibodies-ihc-testing-5.jpgAnti-PKC epsilon/PRKCE Antibody Picoband&reg;IHC analysis of PKC Epsilon/PRKCE using anti-PKC Epsilon/PRKCE antibody (PB9320). <br>PKC Epsilon/PRKCE was detected in a paraffin-embedded section of human stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKC Epsilon/PRKCE Antibody (PB9320) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9320-12967_2014_article_236_fig6_html.jpgAnti-PKC epsilon/PRKCE Antibody Picoband&reg;The effect of SNN on SOCS3 and PKC ε expression in the liver. Male Wistar rats (6 weeks old) were placed on chow diet or HC diet for 8 weeks followed by the 4-week SNN intervention. Livers were collected, the protein expression of SOCS3 and PKCε was analysed by Western blots. Data (mean ± SE) are presented as relative expression levels compared to that of control group. ** P < 0.01 relative to control group, # P < 0.05 in comparison to HC group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12967-014-0236-8'>25160038</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-beta-1-picoband-trade-antibody-pb9319-boster.html2026-03-24T05:04:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9319-prkcb-primary-antibodies-wb-testing-1_1.jpgAnti-PKC beta 1/PRKCB Antibody Picoband&reg;Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (PB9319). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HEL whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC beta 1 antigen affinity purified polyclonal antibody (Catalog # PB9319) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKC beta 1 at approximately 80 kDa. The expected band size for PKC beta 1 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9319-prkcb-primary-antibodies-ip-testing-1.jpgAnti-PKC beta 1/PRKCB Antibody Picoband&reg;Immunoprecipitating (IP) PKC beta 1 in Jurkat whole cell lysate.<br> Western blot analysis of PKC beta 1 using anti-PKC beta 1 antibody (PB9319); <br> Lane 1: Jurkat whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-PKC beta 1 antibody in Jurkat whole cell lysate;<br> Lane 3: anti-PKC beta 1 antibody (2μg) + Jurkat whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PKC beta 1 antigen affinity purified polyclonal antibody (PB9319) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PKC beta 1 at approximately 80 kDa. The expected band size for PKC beta 1 is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9319-prkcb-primary-antibodies-fcm-testing-1.jpgAnti-PKC beta 1/PRKCB Antibody Picoband&reg;Flow Cytometry analysis of Jurkat cells using anti-PKC beta 1 antibody (PB9319). <br> Overlay histogram showing Jurkat cells stained with PB9319 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC beta 1 Antibody (PB9319, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-alpha-picoband-trade-antibody-pb9318-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9318-prkca-primary-antibodies-wb-testing-1.jpgAnti-PKC alpha/PRKCA Antibody Picoband&reg; Western blot analysis of PKC alpha using anti-PKC alpha antibody (PB9318). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates, <br> Lane 2: human HEL whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human U20S whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC alpha antigen affinity purified polyclonal antibody (Catalog # PB9318) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC alpha at approximately 77 kDa. The expected band size for PKC alpha is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9318-2.jpgAnti-PKC alpha/PRKCA Antibody Picoband&reg; IHC analysis of PKC alpha using anti-PKC alpha antibody (PB9318). <br> PKC alpha was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC alpha Antibody (PB9318) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9318-3.jpgAnti-PKC alpha/PRKCA Antibody Picoband&reg; IHC analysis of PKC alpha using anti-PKC alpha antibody (PB9318). <br> PKC alpha was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC alpha Antibody (PB9318) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9318-4.jpgAnti-PKC alpha/PRKCA Antibody Picoband&reg; IHC analysis of PKC alpha using anti-PKC alpha antibody (PB9318). <br> PKC alpha was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PKC alpha Antibody (PB9318) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9318-pkc-primary-antibodies-wb-testing-5.pngAnti-PKC alpha/PRKCA Antibody Picoband&reg; Western blot analysis of PKC alpha/PRKCA using anti-PKC alpha/PRKCA Antibody (PB9318). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 2.5% BSA on 1X-TBST 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC alpha/PRKCA Antibody (PB9318) at 1:1000 (in 0.5%l BSA containing 1X-TBST) and overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a donkey anti-rabbit Ig-Cy5 secondary antibody at a dilution of 1:1000 (in 0.5%l BSA containing 1X-TBST) at 25°C for 2 h. The signal is developed using an CyDye. A specific band was detected for PKC alpha/PRKCA at approximately 76 kDa. The expected band size for PKC alpha/PRKCA is at 76 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-oct-1-picoband-trade-antibody-pb9317-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9317-1-WB-anti-oct-1-picoband-antibody.jpgAnti-Oct-1/POU2F1 Antibody Picoband&reg;Anti-Oct-1 Picoband antibody&#44; PB9317&#44; Western blotting<br>All lanes: Anti Oct-1 (PB9317) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: Human Placenta Tissue Lysate at 50ug<br>Lane 3: JURKAT Whole Cell Lysate at 40ug<br>Lane 4: HELA Whole Cell Lysate at 40ug<br>Lane 5: A549 Whole Cell Lysate at 40ug<br>Lane 6: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 90KD<br>Observed bind size: 90KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9317-2-IHC-anti-oct-1-picoband-antibody.jpgAnti-Oct-1/POU2F1 Antibody Picoband&reg;Anti-Oct-1 Picoband antibody&#44; PB9317&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pin1-picoband-trade-antibody-pb9316-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9316-pin1-primary-antibodies-wb-testing-1_1.jpgAnti-Pin1 Antibody Picoband&reg; Western blot analysis of PIN1 using anti-PIN1 antibody (PB9316). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: monkey COS-7 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat liver tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIN1 antigen affinity purified polyclonal antibody (Catalog # PB9316) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIN1 at approximately 18 kDa. The expected band size for PIN1 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9316-pin1-primary-antibodies-ihc-testing-1.jpgAnti-Pin1 Antibody Picoband&reg;IHC analysis of PIN1 using anti PIN1 antibody (PB9316). <br> PIN1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PIN1 Antibody (PB9316) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9316-pin1-primary-antibodies-if-testing-1.jpgAnti-Pin1 Antibody Picoband&reg;IF analysis of PIN1 using anti-PIN1 antibody (PB9316). <br> PIN1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PIN1 Antibody (PB9316) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9316-pin1-primary-antibodies-fcm-testing-2.jpgAnti-Pin1 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-PIN1 antibody (PB9316). <br>Overlay histogram showing CACO-2 cells stained with PB9316 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIN1 Antibody (PB9316, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pim1-picoband-trade-antibody-pb9315-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9315-pim1-primary-antibodies-wb-testing-1.jpgAnti-PIM1 Antibody Picoband&reg; Western blot analysis of PIM1 using anti-PIM1 antibody (PB9315). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human U20S whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIM1 antigen affinity purified polyclonal antibody (Catalog # PB9315) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIM1 at approximately 44KD. The expected band size for PIM1 is at 44KD. https://www.bosterbio.com/products/primary-antibodies/anti-pik3ca-picoband-trade-antibody-pb9314-boster.html2026-03-16T09:15:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9314-41598_2019_53145_fig3_html.pngAnti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband&reg;( A ) Gene expression analysis of substrates involved in the pathways related to the signaling downstream of insulin (INS) receptors by quantitative RT-PCR. Caco-2 and SW480 carcinoma cells were treated with 5-fluorouracil (FU) and irinotecan (IRI) in the concentration of 500 µM and IRI 50 µM, respectively for 48 h. The mRNA expression of INSR (insulin receptor), IRS 1 (insulin receptor substrate 1), PIK3CA (phosphatidylinositol 3-kinase catalytic subunit alpha), PIK3R1 (phosphatidylinositol 3-kinase regulatory subunit alpha), AKT1 and AKT2 (AKT Serine/Threonine Kinase 1 and 2), MAPK1 (Mitogen-Activated Protein Kinase 1), MAP2K2 (Mitogen-Activated Protein Kinase Kinase 2), GRB2 (Growth factor receptor-bound protein 2), glucose transporters (GLUT-1, GLUT-3, GLUT-4), SREBP-1c (sterol regulatory element-binding protein-1c), GSK3B (Glycogen synthase kinase 3 beta), antiapoptotic protein BCL-2, caspase 3 (CASP3) was determined by quantitative RT-PCR using gene-specific primers. Data are presented as mean ± SD. Experiments were run in triplicate and carried out once. *P < 0.05 compared with control group. ( B ) Western blotting analysis of expression of PIK3CA and GRB2 in Caco-2 and SW480 cancer cell lines. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-019-53145-x'>31719636</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9314-pik3ca-primary-antibodies-wb-testing-1.jpgAnti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband&reg; Western blot analysis of PIK3CA using anti-PIK3CA antibody (PB9314). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CA antigen affinity purified polyclonal antibody (Catalog # PB9314) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3CA at approximately 124 kDa. The expected band size for PIK3CA is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9314-pik3ca-primary-antibodies-fcm-testing-2.jpgAnti-PI 3 Kinase catalytic subunit alpha/PIK3CA Antibody Picoband&reg; Flow Cytometry analysis of Raji cells using anti-PIK3CA antibody (PB9314). <br> Overlay histogram showing Raji cells stained with PB9314 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PIK3CA Antibody (PB9314, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-profilin-1-picoband-trade-antibody-pb9313-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9313-pfn1-primary-antibodies-wb-testing-1.jpgAnti-Profilin 1/PFN1 Antibody Picoband&reg; Western blot analysis of Profilin1 using anti-Profilin1 antibody (PB9313). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates,<br> Lane 6: rat brain tissue lysates,<br> Lane 7: mouse testis tissue lysates,<br> Lane 8: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Profilin1 antigen affinity purified polyclonal antibody (Catalog # PB9313) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Profilin1 at approximately 15 kDa. The expected band size for Profilin1 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9313-2.jpgAnti-Profilin 1/PFN1 Antibody Picoband&reg; IHC analysis of Profilin 1 using anti-Profilin 1 antibody (PB9313). <br> Profilin 1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Profilin 1 Antibody (PB9313) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9313-3.jpgAnti-Profilin 1/PFN1 Antibody Picoband&reg; IHC analysis of Profilin 1 using anti-Profilin 1 antibody (PB9313). <br> Profilin 1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Profilin 1 Antibody (PB9313) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9313-4_1.jpgAnti-Profilin 1/PFN1 Antibody Picoband&reg; IF analysis of Profilin 1 using anti-Profilin 1 antibody (PB9313). <br> Profilin 1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Profilin 1 Antibody (PB9313) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9313-5.pngAnti-Profilin 1/PFN1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-Profilin1 antibody (PB9313). <br> Overlay histogram showing PC-3 cells stained with PB9313 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Profilin1 Antibody (PB9313&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-per2-picoband-trade-antibody-pb9312-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9312-per2-primary-antibodies-wb-testing-1.jpgAnti-PER2 Antibody Picoband&reg; Western blot analysis of PER2 using anti-PER2 antibody (PB9312). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PER2 antigen affinity purified polyclonal antibody (Catalog # PB9312) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PER2 at approximately 130 kDa. The expected band size for PER2 is at 137 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p2x5-picoband-trade-antibody-pb9305-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9305-p2x5-primary-antibodies-wb-testing-1.jpgAnti-P2X5/P2RX5 Antibody Picoband&reg;Western blot analysis of P2X5 using anti-P2X5 antibody (PB9305). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: mouse heart tissue lysates,<br> Lane 4: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X5 antigen affinity purified polyclonal antibody (Catalog # PB9305) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P2X5 at approximately 47 kDa. The expected band size for P2X5 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9305-p2x5-primary-antibodies-ihc-testing-1.jpgAnti-P2X5/P2RX5 Antibody Picoband&reg;IHC analysis of P2X5 using anti-P2X5 antibody (PB9305). <br> P2X5 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-P2X5 Antibody (PB9305) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9305-p2x5-primary-antibodies-if-testing-1.jpgAnti-P2X5/P2RX5 Antibody Picoband&reg;IF analysis of P2X5 using anti-P2X5 antibody (PB9305). <br> P2X5 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-P2X5 Antibody (PB9305) overnight at 4°C. Cy Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdgf-beta-picoband-trade-antibody-pb9311-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9311-1-WB-anti-pdgf-bb-antibody.jpgAnti-PDGF beta/PDGFB Antibody Picoband&reg;Anti-PDGF beta Picoband antibody&#44; PB9311&#44; Western blotting<br>All lanes: Anti PDGF beta (PB9311) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: Rat Brain Tissue Lysate at 50ug<br>Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Lane 4: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 27KD<br>Observed bind size: 13KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pbk-picoband-trade-antibody-pb9310-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9310-pbk-primary-antibodies-wb-testing-1.jpgAnti-PBK Antibody Picoband&reg; Western blot analysis of PBK using anti-PBK antibody (PB9310). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: mouse testis tissue lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBK antigen affinity purified polyclonal antibody (Catalog # PB9310) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBK at approximately 40 kDa. The expected band size for PBK is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9310-2-IHC-anti-pbk-picoband-antibody.jpgAnti-PBK Antibody Picoband&reg; IHC analysis of PBK using anti-PBK antibody (PB9310). <br> PBK was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PBK Antibody (PB9310) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9310-3-IHC-anti-pbk-picoband-antibody.jpgAnti-PBK Antibody Picoband&reg; IHC analysis of PBK using anti-PBK antibody (PB9310). <br> PBK was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PBK Antibody (PB9310) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9310-4-IHC-anti-pbk-picoband-antibody.jpgAnti-PBK Antibody Picoband&reg; IHC analysis of PBK using anti-PBK antibody (PB9310). <br> PBK was detected in a paraffin-embedded section of human mammary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PBK Antibody (PB9310) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9310-5.jpgAnti-PBK Antibody Picoband&reg; IF analysis of PBK using anti-PBK antibody (PB9310). <br> PBK was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PBK Antibody (PB9310) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9310-6.jpgAnti-PBK Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-PBK antibody (PB9310). <br>Overlay histogram showing A549 cells stained with PB9310 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PBK Antibody (PB9310, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-parp-picoband-trade-antibody-pb9309-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp-primary-antibodies-wb-testing-1.jpgAnti-PARP/PARP1 Antibody Picoband&reg; Western blot analysis of PARP using anti-PARP antibody (PB9309). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44;<br> Lane 2: human HepG2 whole cell lysates&#44;<br> Lane 3: human COLO-320 whole cell lysates&#44; <br> Lane 4: human Jurkat whole cell lysates&#44;<br> Lane 5: rat PC-12 whole cell lysates&#44;<br> Lane 6: mouse NIH3T3 whole cell lysates&#44;<br> Lane 7: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP antigen affinity purified polyclonal antibody (Catalog # PB9309) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP at approximately 120KD. The expected band size for PARP is at 113KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-wb-testing-2_2.jpgAnti-PARP/PARP1 Antibody Picoband&reg; Western blot analysis of PARP1 using anti-PARP1 antibody (PB9309). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela- WT whole cell lysates,<br> Lane 2: human Hela-GPX4 KO whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (PB9309) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 118 kDa. The expected band size for PARP1 is at 118 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-3_1.jpgAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PARP using anti-PARP antibody (PB9309). <br> PARP was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-4_1.jpgAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PARP using anti-PARP antibody (PB9309). <br> PARP was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-5_1.jpgAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PARP using anti-PARP antibody (PB9309). <br> PARP was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-6_1.jpgAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PARP using anti-PARP antibody (PB9309).<br> PARP was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-papr-primary-antibodies-ihc-testing-7_1.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-8_1.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-9.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-10.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of human tosil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-11.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-12.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-13.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-ihc-testing-14.pngAnti-PARP/PARP1 Antibody Picoband&reg; IHC analysis of PAPR using anti-PAPR antibody (PB9309). <br> PAPR was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-PAPR Antibody (PB9309) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-wb-testing-15.pngAnti-PARP/PARP1 Antibody Picoband&reg; IF analysis of PARP using anti-PARP antibody (PB9309). <br> PARP was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PARP Antibody (PB9309) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-if-testing-16.pngAnti-PARP/PARP1 Antibody Picoband&reg; IF analysis of PARP using anti-PARP antibody (PB9309). <br> PARP was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PARP Antibody (PB9309) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-if-testing-17.pngAnti-PARP/PARP1 Antibody Picoband&reg; IF analysis of PARP using anti-PARP antibody (PB9309). <br> PARP was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PARP Antibody (PB9309) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp-primary-antibodies-if-testing-18.jpgAnti-PARP/PARP1 Antibody Picoband&reg; IF analysis of PARP using anti-PARP antibody (PB9309). <br> PARP was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-PARP Antibody (PB9309) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Cy3 Conjugated Avidin (BA1037). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9309-parp1-primary-antibodies-fcm-testing-19.pngAnti-PARP/PARP1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-PARP antibody (PB9309). <br> Overlay histogram showing A431 cells stained with PB9309 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PARP Antibody (PB9309, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-park7-dj1-picoband-trade-antibody-pb9308-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9308-park7-primary-antibodies-wb-testing-1.jpgAnti-PARK7/DJ1 Antibody Picoband&reg; Western blot analysis of PARK7 using anti-PARK7 antibody (PB9308). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human PANC-1 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human U251 whole cell lysates,<br> Lane 5: rat kidney tissue lysates,<br> Lane 6: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARK7 antigen affinity purified polyclonal antibody (Catalog # PB9308) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARK7 at approximately 20 kDa. The expected band size for PARK7 is at 20 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9308-2-IHC-anti-park7-dj1-picoband-antibody.jpgAnti-PARK7/DJ1 Antibody Picoband&reg;Anti-PARK7 Picoband antibody&#44; PB9308&#44; IHC(P)<br>IHC(P): Mouse Pancreas Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9308-3-IHC-anti-park7-dj1-picoband-antibody.jpgAnti-PARK7/DJ1 Antibody Picoband&reg;Anti-PARK7 Picoband antibody&#44; PB9308&#44; IHC(P)<br>IHC(P): Rat Pancreas Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9308-4_1-IHC-anti-park7-dj1-picoband-antibody.jpgAnti-PARK7/DJ1 Antibody Picoband&reg;Anti-PARK7 Picoband antibody&#44; PB9308&#44; IHC(P)<br>IHC(P): Human Pancreatic Cancer Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9308-park7-primary-antibodies-if-testing-1.jpgAnti-PARK7/DJ1 Antibody Picoband&reg;IF analysis of PARK7 using anti-PARK7 antibody (PB9308). <br> PARK7 was detected in an immunocytochemical section of SK-OV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PARK7 Antibody (PB9308) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9308-park7-primary-antibodies-ip-testing-1.jpgAnti-PARK7/DJ1 Antibody Picoband&reg;Immunoprecipitating (IP) PARK7 in Hela whole cell lysate.<br> Western blot analysis of PARK7 using anti-PARK7 antibody (PB9308); <br> Lane 1: Hela whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-PARK7 antibody in Hela whole cell lysate;<br> Lane 3: anti-PARK7 antibody (2μg) + Hela whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PARK7 antigen affinity purified polyclonal antibody (PB9308) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PARK7 at approximately 20 kDa. The expected band size for PARK7 is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-parkin-picoband-trade-antibody-pb9307-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9307-1-WB-anti-parkin-picoband-antibody.jpgAnti-Parkin/PRKN Antibody Picoband&reg;Anti-Parkin Picoband antibody&#44; PB9307&#44; Western blotting<br>All lanes: Anti Parkin (PB9307) at 0.5ug/ml<br>Lane 1: U87 Whole Cell Lysate at 40ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 66KD<br>Observed bind size: 66KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9307-2-IHC-anti-parkin-picoband-antibody.jpgAnti-Parkin/PRKN Antibody Picoband&reg;Anti-Parkin Picoband antibody&#44; PB9307&#44; IHC(P)<br>IHC(P): Mouse Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9307-3_1-IHC-anti-parkin-picoband-antibody.jpgAnti-Parkin/PRKN Antibody Picoband&reg;Anti-Parkin Picoband antibody&#44; PB9307&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9307-4-IHC-anti-parkin-picoband-antibody.jpgAnti-Parkin/PRKN Antibody Picoband&reg;Anti-Parkin Picoband antibody&#44; PB9307&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9307-1-s2.0-s2319417025001015-gr3.jpgAnti-Parkin/PRKN Antibody Picoband&reg;Immunohistochemistry of piriformis muscle tissue sections. (A) Parkin expression was higher in the ACP and FSN groups than in the MOD group, with the FSN group showing significantly stronger Parkin immunoreactivity. (B) PINK1 expression was higher in the ACP and FSN groups than in the MOD group, with the FSN group surpassing the ACP group. (C) P62 staining was higher in the MOD and ACP groups than in the COT group. The FSN group showed significantly reduced P62 staining compared to the MOD group. (D-F) Relative protein expression and statistical results. Compared with the COT group, the MOD group had lower Parkin/PINK1 expression and higher P62 expression. Compared with the MOD group, the FSN group had higher Parkin/PINK1 expression and lower P62 expression. Compared with the ACP group, the FSN group showed higher Parkin/PINK1 expression and lower P62 expression. *P<0.05; **P<0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2319417025001015'>41177207</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9307-1-s2.0-s2319417025001015-gr4.jpgAnti-Parkin/PRKN Antibody Picoband&reg;Immunofluorescence of piriformis muscle tissue sections. (A) PINK1 fluorescence intensity was higher in the FSN group than the MOD and ACP groups. (B) Parkin fluorescence intensity was higher in the FSN group than in the MOD and ACP groups. (C) P62 fluorescence intensity was higher in the MOD and ACP groups than in the MOD group. (D-F) Relative protein expression and statistical results. Compared with the COT group, the MOD group had lower PINK1/Parkin expression and higher P62 expression. Compared with the MOD group, the FSN group had higher PINK1/Parkin expression and lower P62 expression. Compared with the ACP group, the FSN group showed higher PINK1/Parkin expression and lower P62 expression. *P<0.05; **P<0.01; ***P<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.sciencedirect.com/science/article/pii/S2319417025001015'>41177207</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9307-5-IF-anti-parkin-picoband-antibody.jpgAnti-Parkin/PRKN Antibody Picoband&reg; IHC analysis of Parkin using anti-Parkin antibody (PB9307).<br> Parkin was detected in immunocytochemical section of NEURO-2α Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Parkin Antibody (PB9307) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-papp-a-picoband-trade-antibody-pb9306-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9306-pappa-primary-antibodies-wb-testing-1.jpgAnti-PAPP A/PAPPA Antibody Picoband&reg;Western blot analysis of PAPPA using anti-PAPPA antibody (PB9306). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human SiHa whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAPPA antigen affinity purified polyclonal antibody (PB9306) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAPPA at approximately 180-220 kDa. The expected band size for PAPPA is at 181 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9306-2-IHC-anti-papp-a-picoband-antibody.jpgAnti-PAPP A/PAPPA Antibody Picoband&reg;Anti-PAPP A Picoband antibody&#44; PB9306&#44; IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p2rx4-picoband-trade-antibody-pb9304-boster.html2026-03-24T05:04:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9304-p2rx4-primary-antibodies-wb-testing-1.jpgAnti-P2RX4 Antibody Picoband&reg; Western blot analysis of P2RX4 using anti-P2RX4 antibody (PB9304). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human A375 whole cell lysates, <br> Lane 4: human T-47D whole cell lysates, <br> Lane 5: human A549 whole cell lysates, <br> Lane 6: monkey COS-7 whole cell lysates, <br> Lane 7: rat NRK whole cell lysates, <br> Lane 8: rat RH-35 whole cell lysates, <br> Lane 9: mouse kidney tissue lysates, <br> Lane 10: mouse liver tissue lysates, <br> Lane 11: mouse Raw264.7 whole cell lysates, <br> Lane 12: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RX4 antigen affinity purified polyclonal antibody (Catalog # PB9304) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RX4 at approximately 72KD. The expected band size for P2RX4 is at 43KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p2x2-picoband-trade-antibody-pb9303-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9303-1-WB-anti-p2x2-picoband-antibody.jpgAnti-P2X2/P2RX2 Antibody Picoband&reg;Anti-P2X2 Picoband antibody&#44; PB9303&#44; Western blotting<br>All lanes: Anti P2X2 (PB9303) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Lane 3: Human Placenta Tissue Lysate at 50ug<br>Lane 4: HELA Whole Cell Lysate at 40ug<br>Lane 5: SHG Whole Cell Lysate at 40ug<br>Lane 6: NEURO Whole Cell Lysate at 40ug<br>Lane 7: 22RV1 Whole Cell Lysate at 40ug<br>Lane 8: U87 Whole Cell Lysate at 40ug<br>Predicted bind size: 52KD<br>Observed bind size: 60KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9303-2-IHC-anti-p2x2-picoband-antibody.jpgAnti-P2X2/P2RX2 Antibody Picoband&reg;Anti-P2X2 Picoband antibody&#44; PB9303&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nup98-picoband-trade-antibody-pb9302-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9302-nup98-primary-antibodies-wb-testing-1.jpgAnti-NUP98 Antibody Picoband&reg; Western blot analysis of NUP98 using anti-NUP98 antibody (PB9302). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human COLO320 whole cell lysates,<br> Lane 5: human HepG2 whole cell lysates,<br> Lane 6: human Hacat whole cell lysates,<br> Lane 7: monkey COS-7 whole cell lysates,<br> Lane 8: rat NRK whole cell lysates,<br> Lane 9: mouse lung tissue lysates,<br> Lane 10: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP98 antigen affinity purified polyclonal antibody (Catalog # PB9302) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP98 at approximately 98 kDa. The expected band size for NUP98 is at 198 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-numb-picoband-trade-antibody-pb9301-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9301-numb-primary-antibodies-wb-testing-1.jpgAnti-NUMB Antibody Picoband&reg; Western blot analysis of NUMB using anti-NUMB antibody (PB9301). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: monkey COS-7 whole cell lysates,<br> Lane 3: human RT4 whole cell lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMB antigen affinity purified polyclonal antibody (Catalog # PB9301) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMB at approximately 71 kDa. The expected band size for NUMB is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9301-numb-primary-antibodies-ihc-testing-2.jpgAnti-NUMB Antibody Picoband&reg; IHC analysis of NUMB using anti-NUMB antibody (PB9301). <br> NUMB was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUMB Antibody (PB9301) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9301-numb-primary-antibodies-ihc-testing-3.jpgAnti-NUMB Antibody Picoband&reg; IHC analysis of NUMB using anti-NUMB antibody (PB9301). <br> NUMB was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NUMB Antibody (PB9301) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9301-numb-primary-antibodies-fcm-testing-4.jpgAnti-NUMB Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-NUMB antibody (PB9301). <br>Overlay histogram showing U87 cells stained with PB9301 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NUMB Antibody (PB9301, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neuropilin-1-picoband-trade-antibody-pb9300-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9300-1-WB-anti-neuropilin-1-antibody.jpgAnti-Neuropilin 1/NRP1 Antibody Picoband&reg;Anti-Neuropillin-1 Picoband antibody&#44; PB9300&#44; Western blotting<br>All lanes: Anti Neuropillin-1 (PB9300) at 0.5ug/ml<br>Lane 1: U87 Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Lane 3: Human Placenta Tissue Lysate at 50ug<br>Lane 4: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Predicted bind size: 103KD<br>Observed bind size: 103KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9300-2-IHC-anti-neuropilin-1-antibody.jpgAnti-Neuropilin 1/NRP1 Antibody Picoband&reg;Anti-Neuropillin-1 Picoband antibody&#44; PB9300&#44; IHC(P)<br> IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nrg1-picoband-trade-antibody-pb9299-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9299-1-WB-anti-neuregulin-1-antibody.jpgAnti-NRG1 Antibody Picoband&reg;Anti-NRG1 Picoband antibody&#44; PB9299&#44; Western blotting<br>All lanes: Anti NRG1 (PB9299) at 0.5ug/ml<br>Lane 1: MCF-7 Whole Cell Lysate at 40ug<br>Lane 2: SKOV Whole Cell Lysate at 40ug<br>Lane 3: 22RV1 Whole Cell Lysate at 40ug<br>Lane 4: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 70KD<br>Observed bind size: 70KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nr5a2-lrh1-picoband-trade-antibody-pb9298-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9298-1-WB-anti-nr5a2-lrh1-picoband-antibody.jpgAnti-NR5A2/LRH1 Antibody Picoband&reg;Anti-NR5A2 Picoband antibody&#44; PB9298&#44; Western blotting<br>All lanes: Anti NR5A2 (PB9298) at 0.5ug/ml<br>Lane 1: PANC Whole Cell Lysate at 40ug<br>Lane 2: HEPG2 Whole Cell Lysate at 40ug<br>Lane 3: A549 Whole Cell Lysate at 40ug<br>Lane 4: SMMC Whole Cell Lysate at 40ug<br>Lane 5: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 61KD<br>Observed bind size: 61KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neuropeptide-y-picoband-trade-antibody-pb9296-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9296-1_1-WB-anti-neuropeptide-y-picoband-antibody.jpgAnti-Neuropeptide Y/NPY Antibody Picoband&reg;All lanes: Anti Neuropeptide Y (PB9296) at 0.5ug/ml<br> WB: Recombinant Human Neuropeptide Y Protein 0.5ng<br> Predicted bind size: 11KD<br> Observed bind size: 11KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9296-npy-primary-antibodies-ihc-testing-5.jpgAnti-Neuropeptide Y/NPY Antibody Picoband&reg;IHC analysis of Neuropeptide Y/NPY using anti-Neuropeptide Y/NPY antibody (PB9296). <br>Neuropeptide Y/NPY was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Neuropeptide Y/NPY Antibody (PB9296) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9296-npy-primary-antibodies-ihc-testing-4.jpgAnti-Neuropeptide Y/NPY Antibody Picoband&reg;IHC analysis of Neuropeptide Y using anti-Neuropeptide Y antibody (PB9296). <br> Neuropeptide Y was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Neuropeptide Y Antibody (PB9296) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9296-2-ihc-anti-neuropeptide-y-picoband-antibody.jpgAnti-Neuropeptide Y/NPY Antibody Picoband&reg;IHC analysis of Neuropeptide Y using anti-Neuropeptide Y antibody (PB9296). <br> Neuropeptide Y was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Neuropeptide Y Antibody (PB9296) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9296-3-ihc-anti-neuropeptide-y-picoband-antibody.jpgAnti-Neuropeptide Y/NPY Antibody Picoband&reg;IHC analysis of Neuropeptide Y using anti-Neuropeptide Y antibody (PB9296). <br> Neuropeptide Y was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Neuropeptide Y Antibody (PB9296) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-anp-picoband-trade-antibody-pb9295-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9295-1-WB-anti-anp-picoband-antibody.jpgAnti-ANP/NPPA Antibody Picoband&reg;Anti-ANP Picoband antibody&#44; PB9295&#44; Western blotting<br>All lanes: Anti ANP (PB9295) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Predicted bind size: 17KD<br>Observed bind size: 17KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-card4-picoband-trade-antibody-pb9294-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9294-1-WB-anti-card4-picoband-antibody.jpgAnti-CARD4/NOD1 Antibody Picoband&reg; Western blot analysis of CARD4 using anti-CARD4 antibody (PB9294). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: A549 Whole Cell Lysate&#44;<br> Lane 2: Rat Cardiac Muscle Tissue Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4 antigen affinity purified polyclonal antibody (Catalog # PB9294) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD4 at approximately 107KD. The expected band size for CARD4 is at 107KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9294-2-IHC-anti-card4-picoband-antibody.jpgAnti-CARD4/NOD1 Antibody Picoband&reg; IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).<br> CARD4 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9294-card4_-primary-antibodies-ihc-testing-3.jpgAnti-CARD4/NOD1 Antibody Picoband&reg; IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).<br> CARD4 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9294-card4_-primary-antibodies-ihc-testing-4.jpgAnti-CARD4/NOD1 Antibody Picoband&reg; IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).<br> CARD4 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9294-card4_-primary-antibodies-ihc-testing-5.jpgAnti-CARD4/NOD1 Antibody Picoband&reg; IHC analysis of CARD4 using anti-CARD4 antibody (PB9294).<br> CARD4 was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CARD4 Antibody (PB9294) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kininogen-1-picoband-trade-antibody-pb9278-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9278-kng1-primary-antibodies-wb-testing-1.jpgAnti-Kininogen 1/KNG1 Antibody Picoband&reg; Western blot analysis of Kininogen 1/KNG1 using anti-Kininogen 1/KNG1 antibody (PB9278). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kininogen 1/KNG1 antigen affinity purified polyclonal antibody (Catalog # PB9278) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kininogen 1/KNG1 at approximately 72 kDa. The expected band size for Kininogen 1/KNG1 is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nkx2-5-picoband-trade-antibody-pb9293-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9293-1-WB-anti-nkx2-5-picoband-antibody.jpgAnti-Nkx2.5/NKX2-5 Antibody Picoband&reg;Anti-NKX2 Picoband antibody&#44; PB9293&#44; Western blotting<br>All lanes: Anti NKX2 (PB9293) at 0.5ug/ml<br>Lane 1: Mouse Spleen Tissue Lysate at 50ug<br>Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 35KD<br>Observed bind size: 35KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-galectin-3-picoband-trade-antibody-pb9279-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9279-lgals3-primary-antibodies-wb-testing-1.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; Western blot analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates,<br> Lane 2: rat stomach tissue lysates,<br> Lane 3: mouse lung tissue lysates,<br> Lane 4: mouse stomach tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 3/LGALS3 antigen affinity purified polyclonal antibody (Catalog # PB9279) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 3/LGALS3 at approximately 29 kDa. The expected band size for Galectin 3/LGALS3 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9279-lgals3-primary-antibodies-ihc-testing-2.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; IHC analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279). <br> Galectin 3/LGALS3 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Galectin 3/LGALS3 Antibody (PB9279) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9279-lgals3-primary-antibodies-ihc-testing-3.jpgAnti-Galectin 3/LGALS3 Antibody Picoband&reg; IHC analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody (PB9279). <br> Galectin 3/LGALS3 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Galectin 3/LGALS3 Antibody (PB9279) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikb-beta-picoband-trade-antibody-pb9292-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9292-ikb-beta-primary-antibodies-wb-testing-1.jpgAnti-IKB beta/NFKBIB Antibody Picoband&reg;Western blot analysis of IKB beta using anti-IKB beta antibody (PB9292). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: rat testis tissue lysates,<br> Lane 5: mouse testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB beta antigen affinity purified polyclonal antibody (PB9292) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IKB beta at approximately 45-48 kDa. The expected band size for IKB beta is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9292-ikb-beta-primary-antibodies-ihc-testing-1.jpgAnti-IKB beta/NFKBIB Antibody Picoband&reg;IHC analysis of IKB beta using anti-IKB beta antibody (PB9292). <br> IKB beta was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IKB beta Antibody (PB9292) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikb-alpha-picoband-trade-antibody-pb9291-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9291-nfkbia-primary-antibodies-wb-testing-1.jpgAnti-IKB alpha/NFKBIA Antibody Picoband&reg;Western blot analysis of IKB Alpha/NFKBIA using anti-IKB Alpha/NFKBIA antibody (PB9291). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human PC-3 whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB Alpha/NFKBIA antigen affinity purified polyclonal antibody (PB9291) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IKB Alpha/NFKBIA at approximately 39 kDa. The expected band size for IKB Alpha/NFKBIA is at 36 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hef1-picoband-trade-antibody-pb9289-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9289-hef1-primary-antibodies-wb-testing-1.jpgAnti-HEF1/NEDD9 Antibody Picoband&reg; Western blot analysis of HEF1 using anti-HEF1 antibody (PB9289). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human Daudi whole cell lysates,<br> Lane 3: monkey COS-7 whole cell lysates,<br> Lane 4: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEF1 antigen affinity purified polyclonal antibody (Catalog # PB9289) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEF1 at approximately 93-105 kDa. The expected band size for HEF1 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9289-hef1-primary-antibodies-wb-testing-2.jpgAnti-HEF1/NEDD9 Antibody Picoband&reg; Western blot analysis of HEF1 using anti-HEF1 antibody (PB9289). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human Daudi whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: rat spleen tissue lysates,<br> Lane 5: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEF1 antigen affinity purified polyclonal antibody (Catalog # PB9289) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEF1 at approximately 93-105 kDa. The expected band size for HEF1 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9289-hef1-primary-antibodies-ihc-testing-3.jpgAnti-HEF1/NEDD9 Antibody Picoband&reg; IHC analysis of HEF1 using anti-HEF1 antibody (PB9289). <br> HEF1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HEF1 Antibody (PB9289) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9289-hef1-primary-antibodies-ihc-testing-4.jpgAnti-HEF1/NEDD9 Antibody Picoband&reg; IHC analysis of HEF1 using anti-HEF1 antibody (PB9289). <br> HEF1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HEF1 Antibody (PB9289) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9289-hef1-primary-antibodies-if-testing-5.jpgAnti-HEF1/NEDD9 Antibody Picoband&reg; IF analysis of HEF1 using anti-HEF1 antibody (PB9289). <br> HEF1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HEF1 Antibody (PB9289) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9289-hef1-primary-antibodies-fcm-testing-6.jpgAnti-HEF1/NEDD9 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-HEF1 antibody (PB9289). <br> Overlay histogram showing A549 cells stained with PB9289 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HEF1 Antibody (PB9289, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-myb-picoband-trade-antibody-pb9288-boster.html2026-03-24T05:04:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9288-c-myb-primary-antibodies-wb-testing-1_1.jpgAnti-c-Myb Antibody Picoband&reg; Western blot analysis of SMC4 using anti-SMC4 antibody (PB9288). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human MOLT-4 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC4 antigen affinity purified polyclonal antibody (PB9288) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMC4 at approximately 70-80 kDa. The expected band size for SMC4 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9288-c-myb-primary-antibodies-ihc-testing-2.jpgAnti-c-Myb Antibody Picoband&reg; IHC analysis of c-Myb using anti-c-Myb antibody (PB9288). <br> c-Myb was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-c-Myb Antibody (PB9288) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9288-c-myb-primary-antibodies-ihc-testing-3.jpgAnti-c-Myb Antibody Picoband&reg; IHC analysis of c-Myb using anti-c-Myb antibody (PB9288). <br> c-Myb was detected in a paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-c-Myb Antibody (PB9288) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9288-c-myb-primary-antibodies-if-testing-1.jpgAnti-c-Myb Antibody Picoband&reg;IF analysis of c-Myb using anti-c-Myb antibody (PB9288). <br> c-Myb was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-c-Myb Antibody (PB9288) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9288-c-myb-primary-antibodies-fcm-testing-4.jpgAnti-c-Myb Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-c-Myb antibody (PB9288). <br> Overlay histogram showing Jurkat cells stained with PB9288 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Myb Antibody (PB9288, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lamin-a-c-picoband-trade-antibody-pb9280-boster.html2026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9280-lamin_a-c-primary-antibodies-wb-testing-1.jpgAnti-Lamin A+C/LMNA Antibody Picoband&reg; Western blot analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: human PC-3 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin A/C antigen affinity purified polyclonal antibody (Catalog # PB9280) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin A/C at approximately 74 kDa, 63 kDa. The expected band size for Lamin A/C is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9280-2-IHC-anti-lamin-a-c-picoband-antibody.jpgAnti-Lamin A+C/LMNA Antibody Picoband&reg; IHC analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). <br> Lamin A/C was detected in a paraffin-embedded section of Mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lamin A/C Antibody (PB9280) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9280-3-IHC-anti-lamin-a-c-picoband-antibody.jpgAnti-Lamin A+C/LMNA Antibody Picoband&reg; IHC analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). <br> Lamin A/C was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lamin A/C Antibody (PB9280) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9280-4-IHC-anti-lamin-a-c-picoband-antibody.jpgAnti-Lamin A+C/LMNA Antibody Picoband&reg; IHC analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). <br> Lamin A/C was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lamin A/C Antibody (PB9280) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9280-5_1.jpgAnti-Lamin A+C/LMNA Antibody Picoband&reg; IF analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). <br> Lamin A/C was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Lamin A/C Antibody (PB9280) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9280-6.jpgAnti-Lamin A+C/LMNA Antibody Picoband&reg; IF analysis of Lamin A/C using anti-Lamin A/C antibody (PB9280). <br> Lamin A/C was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Lamin A/C Antibody (PB9280) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9280-7.jpgAnti-Lamin A+C/LMNA Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-Lamin A/C antibody (PB9280).<br> Overlay histogram showing THP-1 cells stained with PB9280 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lamin A/C Antibody (PB9280,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mesothelin-picoband-trade-antibody-pb9287-boster.html2026-04-01T05:01:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9287-1-WB-anti-mesothelin-picoband-antibody.jpgAnti-Mesothelin/MSLN Antibody Picoband&reg; Western blot analysis of Mesothelin using anti-Mesothelin antibody (PB9287). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Recombinant Human Mesothelin Protein 0.5ng. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody (Catalog # PB9287) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 43KD. The expected band size for Mesothelin is at 43KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9287-2-IHC-anti-mesothelin-picoband-antibody.jpgAnti-Mesothelin/MSLN Antibody Picoband&reg; IHC analysis of Mesothelin using anti-Mesothelin antibody (PB9287).<br> Mesothelin was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Mesothelin Antibody (PB9287) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9287-mesothelin-primary-antibodies-fc-testing-3.jpgAnti-Mesothelin/MSLN Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-Mesothelin antibody (PB9287).<br>Overlay histogram showing SiHa cells stained with PB9287 (Blue line). Mesothelin cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mesothelin Antibody (PB9287&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9287-mesothelin-primary-antibodies-wb-testing-4.jpgAnti-Mesothelin/MSLN Antibody Picoband&reg; Western blot analysis of Mesothelin using anti-Mesothelin antibody (PB9287). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mesothelin antigen affinity purified polyclonal antibody (Catalog # PB9287) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mesothelin at approximately 69KD. The expected band size for Mesothelin is at 69KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mucin-5ac-picoband-trade-antibody-pb9286-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9286-muc5ac-primary-antibodies-ihc-testing-1.jpgAnti-Mucin 5AC/MUC5AC Antibody IHC analysis of Mucin-5AC using anti-Mucin-5AC antibody (PB9286). <br> Mucin-5AC was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mucin-5AC Antibody (PB9286) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9286-muc5ac-primary-antibodies-ihc-testing-2.jpgAnti-Mucin 5AC/MUC5AC Antibody IHC analysis of Mucin-5AC using anti-Mucin-5AC antibody (PB9286). <br> Mucin-5AC was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Mucin-5AC Antibody (PB9286) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lrrk2-picoband-trade-antibody-pb9281-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9281-1-WB-anti-lrrk2-picoband-antibody.jpgAnti-LRRK2 Antibody Picoband&reg;Anti-LRRK2 Picoband antibody&#44; PB9281&#44; Western blotting<br>All lanes: Anti LRRK2 (PB9281) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Lane 3: Rat Liver Tissue Lysate at 50ug<br>Lane 4: U87 Whole Cell Lysate at 40ug<br>Lane 5: NEURO Whole Cell Lysate at 40ug<br>Lane 6: A549 Whole Cell Lysate at 40ug<br>Lane 7: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 286KD<br>Observed bind size: 286 KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-msh2-picoband-trade-antibody-pb9284-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9284-msh2-primary-antibodies-wb-testing-1.jpgAnti-MSH2 Antibody Picoband&reg; Western blot analysis of MSH2 using anti-MSH2 antibody (PB9284). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human HEL whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: human A549 whole cell lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # PB9284) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH2 at approximately 105 kDa. The expected band size for MSH2 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9284-msh2-primary-antibodies-wb-testing-2.jpgAnti-MSH2 Antibody Picoband&reg; Western blot analysis of MSH2 using anti-MSH2 antibody (PB9284). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human SH-SY5Y whole cell lysates, <br> Lane 3: human 293T whole cell lysates, <br> Lane 4: human A549 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSH2 antigen affinity purified polyclonal antibody (Catalog # PB9284) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody (Left) at a dilution of 1:2000 or a goat anti-rabbit IgG-HRP Conjugated secondary antibody (Right) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSH2 approximately 105 kDa. The expected band size for MSH2 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9284-5-IF-anti-msh2-picoband-antibody.jpgAnti-MSH2 Antibody Picoband&reg; IHC analysis of MSH2 using anti-MSH2 antibody (PB9284).<br> MSH2 was detected in immunocytochemical section of SMMC Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-MSH2 Antibody (PB9284) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9284-4-ihc-anti-msh2-picoband-antibody.jpgAnti-MSH2 Antibody Picoband&reg; IHC analysis of MSH2 using anti-MSH2 antibody (PB9284). <br> MSH2 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MSH2 Antibody (PB9284) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9284-5_1.jpgAnti-MSH2 Antibody Picoband&reg; IF analysis of MSH2 using anti-MSH2 antibody (PB9284).<br> MSH2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MSH2 Antibody (PB9284) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9284-6.pngAnti-MSH2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-MSH2 antibody (PB9284). <br> Overlay histogram showing A431 cells stained with PB9284 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSH2 Antibody (PB9284&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mad2l1-picoband-trade-antibody-pb9282-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9282-mad2l1-primary-antibodies-wb-testing-1.jpgAnti-Mad2L1 Antibody Picoband&reg;Western blot analysis of Mad2L1 using anti-Mad2L1 antibody (PB9282). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human THP-1 whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat RH35 whole cell lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mad2L1 antigen affinity purified polyclonal antibody (Catalog # PB9282) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Mad2L1 at approximately 24 kDa. The expected band size for Mad2L1 is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp14-picoband-trade-antibody-pb9283-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9283-1-WB-anti-mmp-14-antibody.jpgAnti-MMP14 Antibody Picoband&reg;Anti-MMP14 Picoband antibody&#44; PB9283&#44; Western blotting<br>All lanes: Anti MMP14 (PB9283) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: HELA Whole Cell Lysate at 40ug<br>Lane 3: U87 Whole Cell Lysate at 40ug<br>Predicted bind size: 55KD<br>Observed bind size: 55KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apolipoprotein-e-picoband-trade-antibody-pb9327-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9327-apoe-primary-antibodies-wb-testing-1.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified polyclonal antibody (Catalog # PB9327) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36 kDa. The expected band size for Apolipoprotein E is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9327-2-IHC-anti-apoe-apolipoprotein-e-antibody.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327). <br> Apolipoprotein E was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Apolipoprotein E Antibody (PB9327) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9327-apoe-primary-antibodies-if-testing-3.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; IF analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9327). <br> Apolipoprotein E was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E Antibody (PB9327) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9327-4.pngAnti-Apolipoprotein E/APOE Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Apolipoprotein E antibody (PB9327). <br> Overlay histogram showing HepG2 cells stained with PB9327 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Apolipoprotein E Antibody (PB9327&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9327-apoe-primary-antibodies-elisa-testing-5.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; Sandwich ELISA - Recombinant human Apolipoprotein E/APOE protein standard curve.<br> Use in combination with reagents from human APOE ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1455). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atp5h-picoband-trade-antibody-pb9328-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9328-atp5h-primary-antibodies-wb-testing-1.jpgAnti-ATP5H Antibody Picoband&reg; Western blot analysis of ATP5H using anti-ATP5H antibody (PB9328). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human U-87MG whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: monkey COS-7 whole cell lysates, <br> Lane 6: human SW620 whole cell lysates, <br> Lane 7: human Jurkat whole cell lysates, <br> Lane 8: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5H antigen affinity purified polyclonal antibody (Catalog # PB9328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22 kDa. The expected band size for ATP5H is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9328-atp5h-primary-antibodies-wb-testing-2.jpgAnti-ATP5H Antibody Picoband&reg; Western blot analysis of ATP5H using anti-ATP5H antibody (PB9328). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: rat heart tissue lysates, <br> Lane 4: rat PC-12 whole cell lysates, <br> Lane 5: mouse brain tissue lysates, <br> Lane 6: mouse liver tissue lysates, <br> Lane 7: mouse heart tissue lysates, <br> Lane 8: mouse ANA-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5H antigen affinity purified polyclonal antibody (Catalog # PB9328) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5H at approximately 22 kDa. The expected band size for ATP5H is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9328-atp5h-primary-antibodies-if-testing-3.jpgAnti-ATP5H Antibody Picoband&reg; IF analysis of ATP5H using anti-ATP5H antibody (PB9328). <br> ATP5H was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATP5H Antibody (PB9328) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9328-atp5h-primary-antibodies-ihc-testing-4.jpgAnti-ATP5H Antibody Picoband&reg; IHC analysis of ATP5H using anti-ATP5H antibody (PB9328). <br> ATP5H was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP5H Antibody (PB9328) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cav1-3-picoband-trade-antibody-pb9329-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9329-1-WB-anti-cav1-3-picoband-antibody.jpgAnti-CaV1.3/CACNA1D Antibody Picoband&reg; Western blot analysis of CAV1.3 using anti-CAV1.3 antibody (PB9329). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44;<br> Lane 2: Mouse Brain Tissue Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV1.3 antigen affinity purified polyclonal antibody (Catalog # PB9329) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CAV1.3 at approximately 245KD. The expected band size for CAV1.3 is at 245KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9329-cav1.3-primary-antibodies-fc-testing-2.jpgAnti-CaV1.3/CACNA1D Antibody Picoband&reg; Flow Cytometry analysis of U-87 cells using anti-CaV1.3 antibody (PB9329). <br>Overlay histogram showing U-87 cells stained with PB9329 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaV1.3 Antibody (PB9329&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calpain-1-picoband-trade-antibody-pb9330-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9330-calpain-1-primary-antibodies-wb-testing-1.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; Western blot analysis of Calpain 1 using anti-Calpain 1 antibody (PB9330). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human PC-3 whole cell lysates, <br> Lane 5: human T47D whole cell lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: rat testis tissue lysates, <br> Lane 8: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 1 antigen affinity purified polyclonal antibody (Catalog # PB9330) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 1 at approximately 82 kDa. The expected band size for Calpain 1 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9330-2-IHC-anti-calpain-1-picoband-antibody.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; IHC analysis of Calpain 1 using anti-Calpain 1 antibody (PB9330). <br> Calpain 1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Calpain 1 Antibody (PB9330) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9330-3.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; IF analysis of Calpain 1 using anti-Calpain 1 antibody (PB9330). <br> Calpain 1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Calpain 1 Antibody (PB9330) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9330-4.jpgAnti-Calpain 1/CAPN1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-Calpain 1 antibody (PB9330).<br>Overlay histogram showing PC-3 cells stained with PB9330 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpain 1 Antibody (PB9330,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-8-picoband-trade-antibody-pb9331-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9331-1-WB-anti-caspase-8-antibody.jpgAnti-Caspase-8/CASP8 Antibody Picoband&reg;Anti-Caspase 8 Picoband antibody&#44; PB9331&#44; Western blotting<br>All lanes: Anti Caspase 8 (PB9331) at 0.5ug/ml<br>Lane 1: Mouse Spleen Tissue Lysate at 50ug<br>Lane 2: Mouse Thymus Tissue Lysate at 50ug<br>Lane 3: Mouse Kidney Tissue Lysate at 50ug<br>Lane 4: Mouse Lung Tissue Lysate at 50ug<br>Lane 5: HEPA Cell Lysate at 40ug<br>Predicted bind size: 55KD<br>Observed bind size: 55KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-9-picoband-trade-antibody-pb9332-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9332-1-WB-anti-caspase-9-picoband-antibody.jpgAnti-Caspase-9/CASP9 Antibody Picoband&reg;Anti-Caspase-9 Picoband antibody&#44; PB9332&#44; Western blotting<br>All lanes: Anti Caspase-9 (PB9332) at 0.5ug/ml<br>Lane 1: A549 Whole Cell Lysate at 40ug<br>Lane 2: SMMC Whole Cell Lysate at 40ug<br>Lane 3: 293T Whole Cell Lysate at 40ug<br>Lane 4: JURKAT Whole Cell Lysate at 40ug<br>Lane 5: RAJI Whole Cell Lysate at 40ug<br>Lane 6: CEM Whole Cell Lysate at 40ug<br>Lane 7: HUT Whole Cell Lysate at 40ug<br>Predicted bind size: 35KD<br>Observed bind size: 35KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd44-picoband-trade-antibody-pb9333-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9333-cd44-primary-antibodies-wb-testing-1.jpgAnti-CD44 Antibody Picoband&reg; Western blot analysis of CD44 using anti-CD44 antibody (PB9333). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human U87 whole cell lysates,<br> Lane 3: rat PC-12 whole cell lysates,<br> Lane 4: rat C6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antigen affinity purified polyclonal antibody (Catalog # PB9333) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD44 at approximately 82 kDa. The expected band size for CD44 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9333-2-IHC-anti-cd44-picoband-antibody.jpgAnti-CD44 Antibody Picoband&reg; IHC analysis of CD44 using anti-CD44 antibody (PB9333). <br> CD44 was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9333-41419_2016_article_bfcddis2016432_fig6_html.jpgAnti-CD44 Antibody Picoband&reg;MiRNAs suppressed cell metastasis in vivo . ( a ) An experimental metastasis model was injected with control miR-206, miR-140, or scrambled control oligos-treated A549/34 R cells. ( b ) Visualization of the HE-stained lung section. Arrow, the migratory A549 cells. ( c ) Immunohistochemistry was conducted to detect CD44 expression. Brown color indicates the migratory A549 cells. Bar=100 μ m <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fcddis2016432'>28005074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9333-3.jpgAnti-CD44 Antibody Picoband&reg; IF analysis of CD44 using anti-CD44 antibody (PB9333) <br> CD44 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9333-4.jpgAnti-CD44 Antibody Picoband&reg; IF analysis of CD44 using anti-CD44 antibody (PB9333) <br> CD44 was detected in paraffin-embedded section of rat lymphaden tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CD44 Antibody (PB9333) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9333-cd44-primary-antibodies-wb-testing-5.jpgAnti-CD44 Antibody Picoband&reg; Western blot analysis of CD44 using anti-CD44 antibody (PB9333). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw) for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD44 antibody ( PB9333) at 1 ug/mL in 5% milk/PBS/0.05% Tween 20 overnight at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit antibody conjugated with HRP at 1:5,000 in 5% milk/PBS/Tw at 4℃ for 12 hours. The signal is developed using an SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific). A specific band was detected for EGFR at approximately 20 kDa. The expected band size for EGFR is at 81 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytochrome-c-picoband-trade-antibody-pb9334-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9334-cycs-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; Western blot analysis of Cytochrome c/CYCS using anti-Cytochrome c/CYCS antibody (PB9334). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: rat kidney tissue lysates, <br> Lane 5: rat skeletal muscle tissue lysates, <br> Lane 6: mouse kidney tissue lysates, <br> Lane 7: mouse skeletal muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytochrome c/CYCS antigen affinity purified polyclonal antibody (PB9334) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytochrome c/CYCS at approximately 14 kDa. The expected band size for Cytochrome c/CYCS is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9334-fphar-10-00175-g006.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg;Trimetazidine inhibited EE-induced apoptosis of myocardial cells. (A) The apoptosis in myocardial tissues was evaluated by TUNEL staining. Scale bar is 50 μm. Western blot analysis of the levels of apoptosis-related proteins B-cell lymphoma 2 (Bcl-2) (B) , Bcl-2-associated X protein (Bax) (C) , cleaved caspase-3 (D) , cleaved PARP (E) , and cytoplasmic cytochrome complex (Cytochrome c, Cyto-C) (F) in myocardial tissues. GAPDH was used as a loading control. Each value is shown as mean ± SD ( n = 6). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, versus the indicated group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00175/full'>30890937</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9334-41598_2017_article_bfsrep42465_fig12_html.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg;Levels of the cytochrome c protein in the cytosol ( A ) and mitochondria ( B ) of the MCF-7/ADR cells after a 24 h incubation with the different formulations (1: blank medium, 2: Pluronic L61 solution, 3: CUR solution, 4: the mixed Pluronic L61/CUR solution, 5: F-pHSM, 6: F-pHSM-L61, 7: F-pHSM/CUR and 8: F-pHSM-L61/CUR). * P < 0.05: significantly different from cells treated with the blank medium, # P < 0.05: significantly different from the F-pHSM-treated cells. The blots were cropped and the full-length blots were included in the . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep42465'>28195164</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9334-2-IHC-anti-cytochrome-c-picoband-antibody.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334). <br> Cytochrome C was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9334-3-IHC-anti-cytochrome-c-picoband-antibody.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334). <br> Cytochrome C was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9334-4-IHC-anti-cytochrome-c-picoband-antibody.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334). <br> Cytochrome C was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9334-5-IF-anti-cytochrome-c-picoband-antibody.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IHC analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334).<br> Cytochrome C was detected in immunocytochemical section of SMMC-7721. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9334-6.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IF analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334) <br> Cytochrome C was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9334-7.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IF analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334) <br> Cytochrome C was detected in paraffin-embedded section of mouse cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9334-8.jpgAnti-Cytochrome C/CYCS Antibody Picoband&reg; IF analysis of Cytochrome C using anti-Cytochrome C antibody (PB9334) <br> Cytochrome C was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Cytochrome C Antibody (PB9334) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmdar2a-picoband-trade-antibody-pb9335-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9335-1-WB-anti-nmdar2a-picoband-antibody.jpgAnti-NMDAR2A/GRIN2A Antibody Picoband&reg;Anti-NMDAR2A Picoband antibody&#44; PB9335&#44; Western blotting<br>All lanes: Anti NMDAR2A (PB9335) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 165KD<br>Observed bind size: 165KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erab-antibodyd-trade-antibody-pb9336-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-hsd17b10-primary-antibodies-wb-testing-1.jpgAnti-ERAB/HSD17B10 Antibody Picoband&reg; Western blot analysis of ERAB using anti-ERAB antibody (PB9336). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human MOLT4 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERAB antigen affinity purified polyclonal antibody (Catalog # PB9336) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERAB at approximately 25 kDa. The expected band size for ERAB is at 27-30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9336-2-IHC-anti-erab-antibody-antibody.jpgAnti-ERAB/HSD17B10 Antibody Picoband&reg; IHC analysis of ERAB using anti-ERAB antibody (PB9336).<br> ERAB was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ERAB Antibody (PB9336) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-erab-primary-antibodies-ihc-testing-3.jpgAnti-ERAB/HSD17B10 Antibody Picoband&reg; IHC analysis of ERAB using anti-ERAB antibody (PB9336).<br> ERAB was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-ERAB Antibody (PB9336) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-erab-primary-antibodies-ihc-testing-4.jpgAnti-ERAB/HSD17B10 Antibody Picoband&reg; IHC analysis of ERAB using anti-ERAB antibody (PB9336).<br> ERAB was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-ERAB Antibody (PB9336) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-erab-primary-antibodies-ihc-testing-5.jpgAnti-ERAB/HSD17B10 Antibody Picoband&reg; IHC analysis of ERAB using anti-ERAB antibody (PB9336).<br> ERAB was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-ERAB Antibody (PB9336) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-erab-primary-antibodies-ihc-testing-6_1.jpgAnti-ERAB/HSD17B10 Antibody Picoband&reg; IHC analysis of ERAB using anti-ERAB antibody (PB9336).<br> ERAB was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-ERAB Antibody (PB9336) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-erab-primary-antibodies-fc-testing-8.pngAnti-ERAB/HSD17B10 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-ERAB antibody (PB9336). <br> Overlay histogram showing A549 cells stained with PB9336 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERAB Antibody (PB9336&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-erab-primary-antibodies-if-testing-7.jpg.jpgAnti-ERAB/HSD17B10 Antibody Picoband&reg; IF analysis of ERAB using anti-ERAB antibody (PB9336). <br> ERAB was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ERAB Antibody (PB9336) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9336-erab-primary-antibodies-fc-testing-9.pngAnti-ERAB/HSD17B10 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ERAB antibody (PB9336). <br> Overlay histogram showing A431 cells stained with PB9336 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERAB Antibody (PB9336&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp60-picoband-trade-antibody-pb9337-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9337-hsp60-primary-antibodies-wb-testing-1.jpgAnti-Hsp60/HSPD1 Antibody Picoband&reg; Western blot analysis of Hsp60 using anti-Hsp60 antibody (PB9337). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human PANC-1 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse liver tissue lysates,<br> Lane 7: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp60 antigen affinity purified polyclonal antibody (Catalog # PB9337) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp60 at approximately 61 kDa. The expected band size for Hsp60 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9337-4-IHC-anti-hsp60-picoband-antibody.jpgAnti-Hsp60/HSPD1 Antibody Picoband&reg;Anti-Hsp60 Picoband antibody&#44; PB9337&#44; IHC(P)<br>IHC(P): Human Intestinal Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9337-3-IHC-anti-hsp60-picoband-antibody.jpgAnti-Hsp60/HSPD1 Antibody Picoband&reg;Anti-Hsp60 Picoband antibody&#44; PB9337&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9337-2-IHC-anti-hsp60-picoband-antibody.jpgAnti-Hsp60/HSPD1 Antibody Picoband&reg;Anti-Hsp60 Picoband antibody&#44; PB9337&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9337-5-IF-anti-hsp60-picoband-antibody.jpgAnti-Hsp60/HSPD1 Antibody Picoband&reg; IHC analysis of Hsp60 using anti-Hsp60 antibody (PB9337).<br> Hsp60 was detected in immunocytochemical section of SMMC-7721 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Hsp60 Antibody (PB9337) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9337-6.jpgAnti-Hsp60/HSPD1 Antibody Picoband&reg; IF analysis of Hsp60 using anti-Hsp60 antibody (PB9337).<br> Hsp60 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hsp60 Antibody (PB9337) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lyric-picoband-trade-antibody-pb9338-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9338-1_1.jpgAnti-LYRIC/MTDH Antibody Picoband&reg; Western blot analysis of LYRIC using anti-LYRIC antibody (PB9338). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: U-87MG whole cell lysates,<br> Lane 2: Hela whole cell lysates,<br> Lane 3: MDA-MB-453 whole cell lysates,<br> Lane 4: PC-3 whole cell lysates,<br> Lane 5: A431 whole cell lysates,<br> Lane 6: CACO-2 whole cell lysates,<br> Lane 7: HepG2 whole cell lysates,<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRIC antigen affinity purified polyclonal antibody (Catalog # PB9338) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LYRIC at approximately 70-80KD. The expected band size for LYRIC is at 70-80KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9338-2-IHC-anti-lyric-picoband-antibody.jpgAnti-LYRIC/MTDH Antibody Picoband&reg; IHC analysis of LYRIC using anti-LYRIC antibody (PB9338).<br> LYRIC was detected in paraffin-embedded section of Rat Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LYRIC Antibody (PB9338) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9338-3-IHC-anti-lyric-picoband-antibody.jpgAnti-LYRIC/MTDH Antibody Picoband&reg; IHC analysis of LYRIC using anti-LYRIC antibody (PB9338).<br> LYRIC was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LYRIC Antibody (PB9338) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9338-lyric-primary-antibodies-ihc-testing-4.jpgAnti-LYRIC/MTDH Antibody Picoband&reg; IHC analysis of LYRIC using anti-LYRIC antibody (PB9338).<br> LYRIC was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-LYRIC Antibody (PB9338) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9338-lyric-primary-antibodies-ihc-testing-5.jpgAnti-LYRIC/MTDH Antibody Picoband&reg; IHC analysis of LYRIC using anti-LYRIC antibody (PB9338).<br> LYRIC was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-LYRIC Antibody (PB9338) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9338-lyric-primary-antibodies-ihc-testing-6.jpgAnti-LYRIC/MTDH Antibody Picoband&reg; IHC analysis of LYRIC using anti-LYRIC antibody (PB9338).<br> LYRIC was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-LYRIC Antibody (PB9338) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-appbp1-picoband-trade-antibody-pb9339-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9339-appbp1-primary-antibodies-wb-testing-1_1.jpgAnti-APPBP1/NAE1 Antibody Picoband&reg; Western blot analysis of APPBP1/NAE1 using anti-APPBP1/NAE1 antibody (PB9339). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: human U20S whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 7: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APPBP1/NAE1 antigen affinity purified polyclonal antibody (Catalog # PB9339) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APPBP1/NAE1 at approximately 60 kDa. The expected band size for APPBP1/NAE1 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmi-picoband-trade-antibody-pb9340-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-1_1.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; Western blot analysis of NMI using anti-NMI antibody (PB9340). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 4: human A431 whole cell lysates,<br> Lane 5: human PC-3 whole cell lysates,<br> Lane 6: human 293T whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMI antigen affinity purified polyclonal antibody (Catalog # PB9340) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMI at approximately 35KD. The expected band size for NMI is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-2_1.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; IHC analysis of NMI using anti-NMI antibody (PB9340). <br> NMI was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NMI Antibody (PB9340) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-3.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; IHC analysis of NMI using anti-NMI antibody (PB9340). <br> NMI was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NMI Antibody (PB9340) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-4.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; IHC analysis of NMI using anti-NMI antibody (PB9340). <br> NMI was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NMI Antibody (PB9340) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-5.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; IHC analysis of NMI using anti-NMI antibody (PB9340). <br> NMI was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NMI Antibody (PB9340) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-6.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; IHC analysis of NMI using anti-NMI antibody (PB9340). <br> NMI was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NMI Antibody (PB9340) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-7.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; IHC analysis of NMI using anti-NMI antibody (PB9340). <br> NMI was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-NMI Antibody (PB9340) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-8.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; IF analysis of NMI using anti-NMI antibody (PB9340). <br> NMI was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-NMI Antibody (PB9340) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9340-9.jpgAnti-N myc interactor/NMI Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-NMI antibody (PB9340).<br> Overlay histogram showing U937 cells stained with PB9340 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NMI Antibody (PB9340,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nucleophosmin-picoband-trade-antibody-pb9341-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9341-npm1-primary-antibodies-wb-testing-1.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; Western blot analysis of Nucleophosmin using anti-Nucleophosmin antibody (PB9341). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: rat NRK whole cell lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: rat RH35 whole cell lysates,<br> Lane 8: mouse ANA-1 whole cell lysates,<br> Lane 9: mouse RAW264.7 whole cell lysates,<br> Lane 10: mouse Neuro-2a whole cell lysates,<br> Lane 11: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleophosmin antigen affinity purified polyclonal antibody (Catalog # PB9341) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nucleophosmin at approximately 39 kDa. The expected band size for Nucleophosmin is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9341-2-IHC-anti-nucleophosmin-picoband-antibody.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PB9341). <br> Nucleophosmin was detected in a paraffin-embedded section of Mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleophosmin Antibody (PB9341) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9341-3-IHC-anti-nucleophosmin-picoband-antibody.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PB9341). <br> Nucleophosmin was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleophosmin Antibody (PB9341) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9341-4-IHC-anti-nucleophosmin-picoband-antibody.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IHC analysis of Nucleophosmin using anti-Nucleophosmin antibody (PB9341). <br> Nucleophosmin was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleophosmin Antibody (PB9341) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9341-npm1-primary-antibodies-if-testing-5.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IF analysis of Nucleophosmin using anti-Nucleophosmin antibody (PB9341). <br> Nucleophosmin was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Nucleophosmin Antibody (PB9341) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9341-npm1-primary-antibodies-if-testing-6.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; IF analysis of Nucleophosmin using anti-Nucleophosmin antibody (PB9341). <br> Nucleophosmin was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Nucleophosmin Antibody (PB9341) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9341-npm1-primary-antibodies-fcm-testing-7_1.jpgAnti-Nucleophosmin/NPM1 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-Nucleophosmin antibody (PB9341). <br> Overlay histogram showing HEL cells stained with PB9341 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nucleophosmin Antibody (PB9341, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nr3c1-picoband-trade-antibody-pb9342-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9342-nr3c1-primary-antibodies-wb-testing-1.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; Western blot analysis of NR3C1 using anti-NR3C1 antibody (PB9342). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: rat liver tissue lysates, <br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C1 antigen affinity purified polyclonal antibody (Catalog # PB9342) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR3C1 at approximately 100 kDa. The expected band size for NR3C1 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9342-2-IHC-anti-nr3c1-glucocorticoid-receptor-picoband-antibody.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9342). <br> NR3C1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9342-3-IHC-anti-nr3c1-glucocorticoid-receptor-picoband-antibody.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9342). <br> NR3C1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9342-4-IHC-anti-nr3c1-glucocorticoid-receptor-picoband-antibody.jpgAnti-Glucocorticoid Receptor/NR3C1 Antibody Picoband&reg; IHC analysis of NR3C1 using anti-NR3C1 antibody (PB9342). <br> NR3C1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NR3C1 Antibody (PB9342) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-optineurin-picoband-trade-antibody-pb9343-boster.html2026-03-24T05:04:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9343-optineurin-primary-antibodies-wb-testing-1.jpgAnti-Optineurin/OPTN Antibody Picoband&reg; Western blot analysis of Optineurin using anti-Optineurin antibody (PB9343). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HT1080 whole cell lysates, <br> Lane 3: huamn HepG2 whole cell lysates, <br> Lane 4: rat heart tissue lysates,<br> Lane 5: rat brain tissue lysates, <br> Lane 6: mouse heart tissuel lysates, <br> Lane 7: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Optineurin antigen affinity purified polyclonal antibody (Catalog # PB9343) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Optineurin at approximately 75 kDa. The expected band size for Optineurin is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9343-optineurin-primary-antibodies-ihc-testing-2.jpgAnti-Optineurin/OPTN Antibody Picoband&reg; IHC analysis of Optineurin using anti-Optineurin antibody (PB9343). <br> Optineurin was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Optineurin Antibody (PB9343) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9343-optineurin-primary-antibodies-ihc-testing-3.jpgAnti-Optineurin/OPTN Antibody Picoband&reg; IHC analysis of Optineurin using anti-Optineurin antibody (PB9343). <br> Optineurin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Optineurin Antibody (PB9343) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9343-optineurin-primary-antibodies-fcm-testing-5_1.jpgAnti-Optineurin/OPTN Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-Optineurin antibody (PB9343). <br>Overlay histogram showing A431 cells stained with PB9343 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Optineurin Antibody (PB9343, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9343-optineurin-primary-antibodies-ihc-testing-4.jpgAnti-Optineurin/OPTN Antibody Picoband&reg; IHC analysis of Optineurin using anti-Optineurin antibody (PB9343). <br> Optineurin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Optineurin Antibody (PB9343) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-otx2-picoband-trade-antibody-pb9344-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9344-1-WB-anti-otx2-picoband-antibody.jpgAnti-Otx2 Antibody Picoband&reg;Anti-Otx2 Picoband antibody&#44; PB9344&#44; Western blotting<br>All lanes: Anti Otx2 (PB9344) at 0.5ug/ml<br>WB: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 32KD<br>Observed bind size: 32KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tpa-picoband-trade-antibody-pb9345-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9345-plat-primary-antibodies-wb-testing-1.jpgAnti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband&reg; Western blot analysis of PLAT using anti-PLAT antibody (PB9345). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human U87 whole cell lysates, <br> Lane 4: human SH-SY5Y whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # PB9345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 63-69 kDa. The expected band size for PLAT is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pp2a-alpha-picoband-trade-antibody-pb9347-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-wb-testing-1.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; Western blot analysis of PPP2CA using anti-PPP2CA antibody (PB9347). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human 293T whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human HepG2 whole cell lysates, <br> Lane 6: rat brain tissue lysates, <br> Lane 7: rat PC-12 whole cell lysates, <br> Lane 8: mouse lung tissue lysates, <br> Lane 9: mouse liver tissue lysates, <br> Lane 10: mouse brain tissue lysates, <br> Lane 11: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2CA antigen affinity purified polyclonal antibody (Catalog # PB9347) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2CA at approximately 34, 36 kDa. The expected band size for PPP2CA is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-ihc-testing-2.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IHC analysis of PPP2CA using anti-PPP2CA antibody (PB9347). <br> PPP2CA was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (PB9347) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-ihc-testing-3.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IHC analysis of PPP2CA using anti-PPP2CA antibody (PB9347). <br> PPP2CA was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (PB9347) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-ihc-testing-4.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IHC analysis of PPP2CA using anti-PPP2CA antibody (PB9347). <br> PPP2CA was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (PB9347) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-ihc-testing-5.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IHC analysis of PPP2CA using anti-PPP2CA antibody (PB9347). <br> PPP2CA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (PB9347) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-ihc-testing-6.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IHC analysis of PPP2CA using anti-PPP2CA antibody (PB9347). <br> PPP2CA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PPP2CA Antibody (PB9347) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-if-testing-7.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; IF analysis of PPP2CA using anti-PPP2CA antibody (PB9347). <br> PPP2CA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP2CA Antibody (PB9347) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9347-ppp2ca-primary-antibodies-fcm-testing-8.jpgAnti-PP2A-alpha/PPP2CA Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-PPP2CA antibody (PB9347). <br> Overlay histogram showing K562 cells stained with PB9347 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP2CA Antibody (PB9347, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-1-picoband-trade-antibody-pb9348-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-prdx1-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; Western blot analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human MDA-MB-453 whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human HepG2 whole cell lysates, <br> Lane 6: human HEK293 whole cell lysates, <br> Lane 7: human placenta tissue lysates, <br> Lane 8: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 1 at approximately 24 kDa. The expected band size for Peroxiredoxin 1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-fnins-14-00181-g002.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg;Prdx1 expression was significantly increased in rats after ICH. (A) Prdx1 expression in the perihematomal area was detected by qRT-PCR (df = 4, F = 35.945, ∗ P < 0.05 versus sham, ∗∗ P < 0.01 versus sham, n = 3). (B) Survival statistics of the sham group and WT group (χ2 = 10.457, df = 1, ∗∗ P = 0.01, n = 20). (C) Prdx1 expression in the perihematomal area was detected by western blot analysis ( F = 2.014, t = –3.432, ∗∗ P < 0.01 versus sham, n = 3). (D) Prdx1 expression in perihematomal tissue was detected by immunohistochemistry (scale bars, 100 μm, F = 1.225, t = –7.288, ∗∗ P < 0.01 versus sham, n = 3). (E) Prdx1 expression in perihematomal tissue was determined by immunofluorescence, and the percentage of positive Prdx1/NeuN, Prdx1/Ibα-1 and Prdx1/GFAP in three randomly chosen fields within the perihematomal area was counted (scale bars, 25 μm, F = 10.964, t = –11.790, ## P < 0.01 versus Prdx1/NeuN. F = 12.000, t = –60.228, ∗∗ P < 0.01 versus Prdx1/NeuN, n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.00181/full'>32210752</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-fnins-14-00181-g003.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg;Prdx1 overexpression alleviated the symptoms of rats after ICH. (A) Survival statistics of the sham group, WT group, Vector group, and Prdx1-OE group (χ2 = 14.310, df = 3, ∗ P < 0.05 versus Vector, # P < 0.05 versus WT, n = 20). (B) Brain water content of the four groups (df = 3, F = 9.324, ∗ P < 0.05 versus Vector, ## P < 0.01 versus WT, n = 6). (C) Hematoma volume of the sham group, the WT group, the Vector group, and the Prdx1-OE group (df = 3, F = 151.467, ∗ P < 0.05 versus Vector, # P < 0.05 versus WT, n = 6). (D) The mNSS was determined for four groups 3 days after ICH (df = 3, F = 75.196, ∗∗ P < 0.01 versus Vector, ## P < 0.01 versus WT, n = 5).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.00181/full'>32210752</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-fnins-14-00181-g004.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg;Prdx1 overexpression inhibited inflammation and apoptosis after ICH. (A) Representative Nissl staining in sham, WT, Prdx1-OE and Vector rats. The number of Nissl-positive cells was assessed (scale bars, 50 μm, df = 3, F = 174.206, ∗∗ P < 0.01 versus Vector; ## P < 0.01 versus WT, n = 3). (B) Representative FJB staining in sham, WT, Prdx1-OE, and Vector rats. The number of FJB-positive cells was assessed (scale bars, 50 μm, df = 3, F = 435.050, ∗∗ P < 0.01 versus Vector; ## P < 0.01 versus WT, n = 3). (C) Western blotting was used to detect Bcl2 and Bax expression in the four groups (df = 3, F = 32.759, ∗ P < 0.05 versus Vector; # P < 0.05 versus WT, n = 3). (D) Comparison of the expression of inflammatory factors in the four groups using qRT-PCR: IL-6 (df = 3, F = 27.046, ∗ P < 0.05 versus Vector; ## P < 0.01 versus WT, n = 3), IL-10 (df = 3, F = 79.041, ∗∗ P < 0.01 versus Vector, ## P < 0.01 versus WT, n = 3), TNF-α (df = 3, F = 10.274, ∗∗ P < 0.01 versus Vector; # P < 0.05 versus WT, n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.00181/full'>32210752</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-fnins-14-00181-g005.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg;The genome-wide landscape of Prdx1 binding sites on RNA. (A) Venn diagram of Prdx1 RIP-seq genes from two biological replicates. (B) Heat map showing the correlation coefficient of two biological replicates. (C) The Prdx1 RIP-seq peaks are predominantly enriched in the CDS region, the 3′ UTR and the 5′ UTR. All RIP-seq peaks were classified according to their distribution on the RNA elements and compared to the genomic background. (D) De novo motif analysis identified GA repeat and GA-enriched sequences as Prdx1 binding motifs. (E) Prdx1 RIP-seq peak distribution proportion. (F) Prdx1 RIP-seq peaks are shown as track signals. The peak area is indicated by the black frame. (G) RIP-qPCR analysis of Prdx1 binding RNAs, IgG RIP was negative RIP control (BCL6: F = 3.745, t = –6.708, ∗∗ P < 0.01 versus IgG, n = 3; TLR6: F = 1.668, t = –7.065, ∗∗ P < 0.01 versus IgG, n = 3; FOS: F = 5.224, t = –10.432, ∗∗ P < 0.01 versus IgG, n = 3; PTEN: F = 0.000, t = –6.998, ∗∗ P < 0.01 versus IgG, n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.00181/full'>32210752</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-fnins-14-00181-g006.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg;Prdx1 affects inflammation- and apoptosis-related mRNA stability. (A) Prdx1 expression in control HeLa cells and in the Prdx1-OE group was detected by qRT-PCR ( F = 8.494, t = 40.635, ∗∗ P < 0.01 versus Control). (B) Scatter plots and correlation coefficients of two biological replicates of RNA-seq. (C) Volcano map: red dot indicates a gene that is upregulated after Prdx1 overexpression, black dot indicates a gene with no significant change, and blue dot indicates a downregulated gene. (D) Gene ontology (GO) analysis of Prdx1-dependent DEGs. Significantly enriched GO terms of genes. The x -axis indicates the enrichment P -value on a −log10 scale; the y -axis indicates terms. (E) Heat map showing that apoptosis- and inflammation-related genes were significantly decreased after Prdx1 was upregulated in HeLa cells. (F) qRT-PCR was performed in four groups 3 days after ICH (BMP2: F = 8.949, df = 3, ∗ P < 0.05 versus Vector, # P < 0.05 versus WT, n = 3; CCL2: F = 33.103, df = 3, ∗∗ P < 0.01 versus Vector, ## P < 0.01 versus WT, n = 3; TLR3: F = 39.330, df = 3, ∗∗ P < 0.01 versus Vector, ## P < 0.01 versus WT, n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.00181/full'>32210752</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-fnins-14-00181-g007.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg;Prdx1 plays similar roles in vitro and in vivo . (A) Prdx1 RIP-seq peaks are shown as track signals of ANGPTL4, GADD45A and THBS1. The peak area is indicated by the black frame. (B) RIP-qPCR analysis of Prdx1 binding RNAs in astrocytes with IgG RIP as a negative RIP control (ANGPTL4: F = 4.297, t = –13.551, ∗∗ P < 0.01 versus IgG, n = 3; GADD45A: F = 12.277, t = –8.909, ∗∗ P < 0.01 versus IgG, n = 3; THBS: F = 4.072, t = –20.619, ∗∗ P < 0.01 versus IgG, n = 3). (C) qRT-PCR was performed in four groups of ANGPTL4, GADD45A, and THBS1 mRNA (ANGPTL4: F = 87.049, df = 3, ∗ P < 0.05 versus Vector, # P < 0.05 versus WT, n = 3; GADD45A: F = 73.252, df = 3, ∗∗ P < 0.01 versus Vector, ## P < 0.01 versus WT, n = 3; THBS: F = 20.553, df = 3, ∗∗ P < 0.01 versus Vector, ## P < 0.01 versus WT, n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.00181/full'>32210752</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-prdx1-primary-antibodies-wb-testing-2.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; Western blot analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: rat testis tissue lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: rat PC-12 whole cell lysates, <br> Lane 5: mouse kidney tissue lysates, <br> Lane 6: mouse testis tissue lysates, <br> Lane 7: mouse liver tissue lysates, <br> Lane 8: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 1 at approximately 24 kDa. The expected band size for Peroxiredoxin 1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-prdx1-primary-antibodies-fcm-testing-9.pngAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; Flow Cytometry analysis of HEPG2 cells using anti-Peroxiredoxin 1 antibody (PB9348). <br>Overlay histogram showing HEPG2 cells stained with PB9348 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 1 Antibody (PB9348, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-3-ihc-anti-peroxiredoxin-1-picoband-antibody.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348). <br> Peroxiredoxin 1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Peroxiredoxin 1 Antibody (PB9348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-4-ihc-anti-peroxiredoxin-1-picoband-antibody.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348). <br> Peroxiredoxin 1 was detected in a paraffin-embedded section of Mouse Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Peroxiredoxin 1 Antibody (PB9348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-5-ihc-anti-peroxiredoxin-1-picoband-antibody.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348). <br> Peroxiredoxin 1 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Peroxiredoxin 1 Antibody (PB9348) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-6-icc-anti-peroxiredoxin-1-picoband-antibody.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348).<br> Peroxiredoxin 1 was detected in immunocytochemical section of SMMC-7721 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 1 Antibody (PB9348) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-7.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; IF analysis of Peroxiredoxin 1 using anti-Peroxiredoxin 1 antibody (PB9348). <br> Peroxiredoxin 1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 1 Antibody (PB9348) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9348-prdx1-primary-antibodies-if-testing-8.jpgAnti-Peroxiredoxin 1/PRDX1 Antibody Picoband&reg; IF analysis of Peroxiredoxin 1 using anti- Peroxiredoxin 1 antibody (PB9348). <br> Peroxiredoxin 1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 1 Antibody (PB9348) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-3-picoband-trade-antibody-pb9349-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9349-prdx3-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg;Western blot analysis of PRDX3 using anti-PRDX3 antibody (PB9349). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human LN229 whole cell lysates,<br> Lane 2: rat C6 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human 293T whole cell lysates,<br> Lane 6: human CACO-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX3 antigen affinity purified polyclonal antibody (PB9349) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRDX3 at approximately 25 kDa. The expected band size for PRDX3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9349-2-IHC-anti-peroxiredoxin-3-picoband-antibody.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody (PB9349).<br> Peroxiredoxin 3 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peroxiredoxin 3 Antibody (PB9349) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9349-peroxiredoxin_3-primary-antibodies-ihc-testing-3.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody (PB9349).<br> Peroxiredoxin 3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 3 Antibody (PB9349) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9349-peroxiredoxin_3-primary-antibodies-ihc-testing-4.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody (PB9349).<br> Peroxiredoxin 3 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 3 Antibody (PB9349) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9349-peroxiredoxin_3-primary-antibodies-ihc-testing-5.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody (PB9349).<br> Peroxiredoxin 3 was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 3 Antibody (PB9349) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9349-peroxiredoxin_3-primary-antibodies-ihc-testing-6.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; IF analysis of Peroxiredoxin 3 using anti-Peroxiredoxin 3 antibody (PB9349). <br> Peroxiredoxin 3 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 3 Antibody (PB9349) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9349-peroxiredoxin_3-primary-antibodies-fcm-testing-7.jpgAnti-Peroxiredoxin 3/PRDX3 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-Peroxiredoxin 3 antibody (PB9349).<br>Overlay histogram showing U937 cells stained with PB9349 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 3 Antibody (PB9349,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-6-picoband-trade-antibody-pb9350-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9350-2-IHC-anti-peroxiredoxin-6-picoband-antibody.jpgAnti-Peroxiredoxin 6/PRDX6 Antibody Picoband&reg;Anti-Peroxiredoxin 6 Picoband antibody&#44; PB9350&#44; IHC(P)<br> IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9350-prdx6-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 6/PRDX6 Antibody Picoband&reg; Western blot analysis of PRDX6 using anti-PRDX6 antibody (PB9350). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse lung tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX6 antigen affinity purified polyclonal antibody (Catalog # PB9350) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 25 kDa. The expected band size for PRDX6 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9350-3.jpgAnti-Peroxiredoxin 6/PRDX6 Antibody Picoband&reg; ICC analysis of PRDX6 using anti-PRDX6 antibody (PB9350).<br> PRDX6 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-PRDX6 Antibody (PB9350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9350-4.jpgAnti-Peroxiredoxin 6/PRDX6 Antibody Picoband&reg; IHC analysis of PRDX6 using anti-PRDX6 antibody (PB9350). <br> PRDX6 was detected in paraffin-embedded section of human gastric cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDX6 Antibody (PB9350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9350-5.jpgAnti-Peroxiredoxin 6/PRDX6 Antibody Picoband&reg; IHC analysis of PRDX6 using anti-PRDX6 antibody (PB9350). <br> PRDX6 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDX6 Antibody (PB9350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9350-6.jpgAnti-Peroxiredoxin 6/PRDX6 Antibody Picoband&reg; IHC analysis of PRDX6 using anti-PRDX6 antibody (PB9350). <br> PRDX6 was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDX6 Antibody (PB9350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9350-7.jpgAnti-Peroxiredoxin 6/PRDX6 Antibody Picoband&reg; IHC analysis of PRDX6 using anti-PRDX6 antibody (PB9350). <br> PRDX6 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PRDX6 Antibody (PB9350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psca-picoband-trade-antibody-pb9351-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9351-1-WB-anti-psca-picoband-antibody.jpgAnti-PSCA Antibody Picoband&reg;Anti-PSCA Picoband antibody&#44; PB9351&#44; Western blotting<br>All lanes: Anti PSCA (PB9351) at 0.5ug/ml<br>Lane 1: Rat Stomach Tissue Lysate at 50ug<br>Lane 2: Mouse Stomach Tissue Lysate at 50ug<br>Predicted bind size: 13KD<br>Observed bind size: 25KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-semaphorin-3a-picoband-trade-antibody-pb9353-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9353-1-WB-anti-semaphorin-3a-picoband-antibody.jpgAnti-Semaphorin 3A/SEMA3A Antibody Picoband&reg;Anti-Semaphorin 3A Picoband antibody&#44; PB9353&#44; Western blotting<br>All lanes: Anti Semaphorin 3A (PB9353) at 0.5ug/ml<br>Lane 1: 293T Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 89KD<br>Observed bind size: 89KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serpina1-picoband-trade-antibody-pb9354-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9354-serpina1-primary-antibodies-wb-testing-1.jpgAnti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband&reg; Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (PB9354). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: rat lung tissue lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: mouse kidney tissue lysates, <br> Lane 5: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (Catalog # PB9354) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 53 kDa. The expected band size for SERPINA1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9354-2-IHC-anti-serpina1-alpha-1-antitrypsin-picoband-antibody.jpgAnti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband&reg; IHC analysis of SERPINA1 using anti-SERPINA1 antibody (PB9354). <br> SERPINA1 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SERPINA1 Antibody (PB9354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9354-3-IHC-anti-serpina1-alpha-1-antitrypsin-picoband-antibody.jpgAnti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband&reg; IHC analysis of SERPINA1 using anti-SERPINA1 antibody (PB9354). <br> SERPINA1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SERPINA1 Antibody (PB9354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serpinb2-picoband-trade-antibody-pb9355-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9355-1-WB-anti-serpinb2-picoband-antibody.jpgAnti-SerpinB2 Antibody Picoband&reg;Anti-SerpinB2 Picoband antibody&#44; PB9355&#44; Western blotting<br>All lanes: Anti SerpinB2 (PB9355) at 0.5ug/ml<br>WB: Human Placenta Tissue Lysate at 50ug<br>Predicted bind size: 47KD<br>Observed bind size: 47KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9355-2-IHC-anti-serpinb2-picoband-antibody.jpgAnti-SerpinB2 Antibody Picoband&reg;Anti-SerpinB2 Picoband antibody&#44; PB9355&#44;IHC(P)<br>IHC(P): Human Placenta Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-maspin-picoband-trade-antibody-pb9356-boster.html2026-04-01T05:01:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9356-serpinb5-primary-antibodies-wb-testing-1.jpgAnti-MASPIN/SERPINB5 Antibody Picoband&reg; Western blot analysis of MASPIN/SERPINB5 using anti-MASPIN/SERPINB5 antibody (PB9356). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Hacat whole cell lysates,<br> Lane 3: human CACO-2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASPIN/SERPINB5 antigen affinity purified polyclonal antibody (Catalog # PB9356) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MASPIN/SERPINB5 at approximately 42 kDa. The expected band size for MASPIN/SERPINB5 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9356-serpinb5-primary-antibodies-ihc-testing-2.jpgAnti-MASPIN/SERPINB5 Antibody Picoband&reg; IHC analysis of MASPIN/SERPINB5 using anti-MASPIN/SERPINB5 antibody (PB9356). <br> MASPIN/SERPINB5 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MASPIN/SERPINB5 Antibody (PB9356) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9356-serpinb5-primary-antibodies-ihc-testing-3.jpgAnti-MASPIN/SERPINB5 Antibody Picoband&reg; IHC analysis of MASPIN/SERPINB5 using anti-MASPIN/SERPINB5 antibody (PB9356). <br> MASPIN/SERPINB5 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MASPIN/SERPINB5 Antibody (PB9356) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9356-serpinb5-primary-antibodies-if-testing-4.jpgAnti-MASPIN/SERPINB5 Antibody Picoband&reg; IF analysis of MASPIN/SERPINB5 using anti-MASPIN/SERPINB5 antibody (PB9356). <br> MASPIN/SERPINB5 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MASPIN/SERPINB5 Antibody (PB9356) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sirt6-picoband-trade-antibody-pb9357-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9357-sirt6-primary-antibodies-wb-testing-1.jpgAnti-SIRT6 Antibody Picoband&reg; Western blot analysis of SIRT6 using anti-SIRT6 antibody (PB9357). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human U2OS whole cell lysates,<br> Lane 5: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT6 antigen affinity purified polyclonal antibody (Catalog # PB9357) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT6 at approximately 39-42 kDa. The expected band size for SIRT6 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9357-2-IHC-anti-sirt6-picoband-antibody.jpgAnti-SIRT6 Antibody Picoband&reg;Anti-SIRT6Picoband antibody&#44; PB9357&#44; IHC(P)<br>IHC(P): Mouse Cardiac Muscle Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9357-3-IHC-anti-sirt6-picoband-antibody.jpgAnti-SIRT6 Antibody Picoband&reg;Anti-SIRT6Picoband antibody&#44; PB9357&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sirt7-picoband-trade-antibody-pb9358-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9358-sirt7-primary-antibodies-wb-testing-1.jpgAnti-SIRT7 Antibody Picoband&reg; Western blot analysis of SIRT7 using anti-SIRT7 antibody (PB9358). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: rat spleen tissue lysates, <br> Lane 4: rat thymus tissue lysates, <br> Lane 5: mouse thyums tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT7 antigen affinity purified polyclonal antibody (Catalog # PB9358) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT7 at approximately 40 kDa. The expected band size for SIRT7 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9358-2-IHC-anti-sirt7-picoband-antibody.jpgAnti-SIRT7 Antibody Picoband&reg; IHC analysis of SIRT7 using anti-SIRT7 antibody (PB9358). <br> SIRT7 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT7 Antibody (PB9358) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9358-3-IHC-anti-sirt7-picoband-antibody.jpgAnti-SIRT7 Antibody Picoband&reg; IHC analysis of SIRT7 using anti-SIRT7 antibody (PB9358). <br> SIRT7 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT7 Antibody (PB9358) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vimentin-picoband-trade-antibody-pb9359-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-wb-testing-1_1.jpgAnti-Vimentin Antibody Picoband&reg; Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human U-87MG whole cell lysates, <br> Lane 3: human U20S whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: human Hela whole cell lysates,<br> Lane 6: human THP-1 whole cell lysates,<br> Lane 7: human A549 whole cell lysates,<br> Lane 8: human MCF-7 whole cell lysates,<br> Lane 9: rat ovary tissue lysates,<br> Lane 10: rat lung tissue lysates,<br> Lane 11: mouse lung tissue lysates,<br> Lane 12: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # PB9359) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-wb-testing-2.jpgAnti-Vimentin Antibody Picoband&reg;Western blot analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1-4: control group-human HTR8 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (PB9359) at 1:2500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substratewith ChemiDoc MP system. A specific band was detected for Vimentin at approximately 54 kDa. The expected band size for Vimentin is at 54 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9359-2-IHC-anti-vimentin-picoband-antibody.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9359-3-IHC-anti-vimentin-picoband-antibody.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9359-4-IHC-anti-vimentin-picoband-antibody.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-ihc-testing-5.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-ihc-testing-6.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-ihc-testing-7.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-ihc-testing-8.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of human invasive urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-ihc-testing-9.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-ihc-testing-10.jpgAnti-Vimentin Antibody Picoband&reg; IHC analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-if-testing-11.jpgAnti-Vimentin Antibody Picoband&reg; IF analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-vimentin-primary-antibodies-if-testing-12.jpgAnti-Vimentin Antibody Picoband&reg; IF analysis of Vimentin using anti-Vimentin antibody (PB9359). <br> Vimentin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Vimentin Antibody (PB9359) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-41419_2018_986_fig4_html.pngAnti-Vimentin Antibody Picoband&reg;MiR-3619 induces p21, downregulates β-catenin and CDK2 expression and inhibits the EMT process. a p21, E-cadherin, and Cyclin D1 mRNA expression were detected by qRT-PCR. b , c The expression of proteins from potential miR-3619 targets is shown in a western blot of BCa cells from 3 days after transfection of miR-3619 or controls. d , e Immunofluorescence staining of β-catenin in 5637 and T24 cells. f N-cadherin, Vimentin, and Snail mRNA expression in 5637 and T24 cells were measured using qRT-PCR. g The N-cadherin, Vimentin, and Snail protein expression in 5637 and T24 cells were measured using western blot. * P < 0.05, ** P < 0.01 compared with the dsControl group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-018-0986-y'>30237499</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-41598_2018_24888_fig1_html.jpgAnti-Vimentin Antibody Picoband&reg;Generation of 3D-spheroid GMSCs. ( A ) 4 × 10 4 of GMSCs/well in 200 µl of complete stemgro culture medium was seeded into each well of ultra-low attachment round-shaped 96-U well plates and cultured for 48 h. H & E staining of cryosections of GMSC spheroids. Scale bar: 50 µm. ( B ) Immunocytochemistry showed the expression of a panel of MSC-associated markers CD29, CD73 and CD90 and extracellular components such as type I collagen (col-I), vimentin, fibronectin, and laminin in GMSC-derived spheroids. ( C ) GMSC spheroids were dissociated into single cells and stained with Annexin V-FITC and 7-AAD to detect early apoptosis and late apoptotic/necrotic cells by flow cytometric analysis. ( D ) Immunocytochemistry showed that less than 10% of cells inside GMSC spheroids were positive for the cleaved caspase-3. Cell nuclei were counter-stained by DAPI (blue). Scale bar: 50 µm. Data are representative of 3 independent experiments. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-018-24888-w'>29700345</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-13046_2019_1175_fig3_html.pngAnti-Vimentin Antibody Picoband&reg;MiR-139 suppresses HCC migration and invasion. a - e Hep3B and SMMC7721 cells were transfected with 100 nM miR-139 mimic oligos or control oligos. The migration capacity of ( a ) Hep3B and ( b ) SMMC7721 cells were analyzed with the transwell migration assay. The invasion ability of ( c ) Hep3B and ( d ) SMMC7721 cells were measured with the matrigel transwell invasion assay. e The protein expression levels of E-cadherin, Vimentin and SNAIL1 were assayed with western blot in both Hep3B and SMMC7721 cells. f Hep3B and SMMC7721 cells were transfected with 100 nM miR-139 inhibitory or control oligos. The protein levels of E-cadherin, Vimentin and SNAIL1 were measured by western blot in both cell lines. All experiments were repeated 3 times. ** P < 0.01, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1175-2'>31046781</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-13046_2019_1175_fig6_html.pngAnti-Vimentin Antibody Picoband&reg;Down-regulating KPNA2 leads to decreased growth of HCC cells. Hep3B cells were transfected with non-targeting control oligo or with siRNA specifically targeting KPNA2 using lipofectamine 2000. a KPNA2 mRNA level was measured with qRT-PCR. b Western blot was used to assay the protein level of KPNA2. c Cell viability was measured using the MTT assay. d Cell apoptosis was measured with the Annxin V-FITC/Hoechst 33258 double staining flow cytometry. The cells were incubated for 72 h after transfection of the oligos. e colonogenic, ( f ) migratory and ( g ) invading capacities of cells were assayed as described above. h The protein levels of E-cadherin and Vimentin were measured using western blot. i The OS of HCC patients with low or high KPNA2 level was analyzed with Kaplan-Meier survival analysis and the curves were compared with the Log-rank test. The Q1–4 represent the 25% quantile of KPNA2 expression. Q1 represents the lowest 25%, Q2 represents 25–50%, Q3 is the 50–75% and Q4 is the 75–100%. All experiments were repeated 3 times. * P < 0.05, ** P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13046-019-1175-2'>31046781</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-fonc-10-00144-g003.jpgAnti-Vimentin Antibody Picoband&reg;LRRC4 inhibits collective cells invasion by down-regulating E-cadherin without EMT induction. (A) Phalloidin staining was performed in SKOV3 cells stably expressing LRRC4 through lentivirus infection. F-actin aggregates along control cell peripherals, while LRRC4 inhibites the accumulation and aggregation of F-actin (indicated with arrowheads). (B) The mRNA levels of EMT associated-genes, including E-cadherin, N-cadherin, slug, twist, ZEB1 and ZEB2, as assessed with RT-qPCR. (C) Western blotting analysis of E-cadherin and Vimentin protein in SKOV3 cells transiently transfected with different doses of LRRC4 expression vector (0.5, 1, and 2 μg). (D,E) E-cadherin and LRRC4 protein expression and localization as detected with immunohistochemistry (IHC) in human normal ovarian epithelial cells ( D , n = 5), human HGSC ( E , n = 17) and ascites of ovarian cancer patients. Scale bars: the left is 50 μm while the right is 20 μm. The area in the red boxes to the right is magnified. (F) E-cadherin and LRRC4 in EOC ascitic tissue as stained with IHC. Scale bars: 20 μm. (G) Expression of E-cadherin, Vimentin and Pan-cadherin in intraperitoneal xenografted primary tumor tissues from the mouse model was examined by the use of IHC in. Scale bars: 20 μm and 50 μm. * p < 0.05, ** p < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2020.00144/full'>32117780</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-ijmsv17p0137g001.jpgAnti-Vimentin Antibody Picoband&reg;Key characteristics of the immortalized HP-1 human PSC cell line. Phase contrast microscopy in HP-1 cells after seeding for 12 h ( A ); Double-immunofluorescence labelling using antibodies for SV40 antigen and GFAP, green, localization of SV40 antigen, red, localization of GFAP (B); Immunofluorescence showing the presence of α-SMA ( C ); Double-immunofluorescence labelling for vimentin and desmin. Green, localization of vimentin (D), red, localization of desmin (E), yellow, colocalization of vimentin and desmin (F). (Original magnification × 200 in A ; × 400 in B-F ).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6945563/&orig_host=www.ncbi.nlm.nih.gov'>31929747</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-41419_2020_3300_fig5_html.pngAnti-Vimentin Antibody Picoband&reg;ZFPM2-AS1 promoted cell metastatic properties by affecting EMT in HCC cells. A , B The migration abilities were evaluated in HCC cells infected with sh-ZFPM2-AS1-1 or sh-ZFPM2-AS1-2 lentivirus, by wound-healing assays. Scale bar = 50 μm. C Transwell assays detected the invasion capacities of HCC cells after ZFPM2-AS1-2 depletion. Scale bar = 50 μm. D Western blot examined the protein levels of N-cadherin and vimentin in HCC cells. Data are presented as the mean ± SD from three independent experiments. * P < 0.05, ** P < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-03300-4'>33414427</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-12906_2021_3213_fig1_html.pngAnti-Vimentin Antibody Picoband&reg;The characterization of PDLCs. Primary culture of PDLCs at the Day 7 (×100) ( a ); Morphology of PDLCs at Passage 1 (× 100) ( b ); Immunohistochemistry staining results of PDLCs, positive for vimentin (× 100) ( c ) and negative for cytokeratin (× 100) ( d ) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12906-021-03213-5'>33485352</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-41419_2022_5482_fig2_html.pngAnti-Vimentin Antibody Picoband&reg;LPC inhibits pulmonary metastasis of CT26 colon cancer. a Schematic view of the experimental procedures of CT26 pulmonary metastatic mouse model. b , c Image and corresponding H&E staining of lung tissue. d Percentage change of body weight. e – g Lung weight, the number of lung tumor nodule, and metastasis rate ( n = 6 mice). h Protein expression of PCNA, vimentin and E-cadherin in lungs ( n = 3 mice). Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-022-05482-5'>36774343</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-41598_2024_75938_fig4_html.pngAnti-Vimentin Antibody Picoband&reg;The combination of AO/854 and CDDP could effectively suppress the ability of metastasis and invasion in PC3 cells. ( A-B ) Inhibitory effects of AO/854 (2.5 µmol/L) and CDDP (2.5 µmol/L) on the migration activity of PC3 cells. Scale bars: 50 μm. ( C-D ) Inhibitory effects of AO/854 (2.5 µmol/L) and CDDP (2.5 µmol/L) on invasion activity of PC3 cells. Scale bars: 50 μm. ( E-F ) The results of WB for E-cadherin, N-cadherin, and vimentin. PC3 cells were treated with AO/854 (2.5 µmol/L) and CDDP (2.5 µmol/L) for 48 h. GAPDH was examined as a loading control. The presented results were representative of experiments repeated at least three times. Data were represented as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001). Original blots are presented in Figs. – . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-75938-5'>39438537</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9359-j_chem-2018-0042_fig_002.jpgAnti-Vimentin Antibody Picoband&reg;Western blot results showing the expression levels of A549 cells treated with different concentrations of GLXB-D drug serum and TGF-β1 for 24 h . (A): The protein expression straps of E-cadherin and Vimentin; (B): The grey value ratio of expressions of E-cadherin and Vimentin. (1) control group; (2) 5ng/mL TGF-β1 induced group; (3) 5% GLXB-D drug serum group; (4) 10% GLXB-D drug serum group; (5) 15% GLXB-D drug serum group. <br><b>Index in De Gruyter Brill under a CC BY license. DOI: <a href='https://www.degruyterbrill.com/document/doi/10.1515/chem-2018-0042/html'>10.1515/chem-2018-0042</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fe65-antibody-rp1059-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1059-1-WB-anti-fe65-antibody.jpgAnti-FE65/APBB1 Antibody Picoband&reg;Anti-FE65 antibody&#44; RP1059&#44; Western blotting<br>All lanes: Anti (RP1059) at 0.5ug/ml<br>WB: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 77KD<br>Observed bind size: 77KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hgf-antibody-rp1062-boster.html2026-03-24T05:04:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1062-13019_2022_1861_fig2_html.pngAnti-Hepatocyte growth factor HGF Antibody Picoband&reg;HGF is a direct target of miR-144-3p. a HGF 3′-UTR was a direct target of miR-144-3p. b Luciferase reporter assay detected the luciferase activity of cells co-transfected with the wild-type (WT) or mutant (Mut) HGF 3′-UTR reporter genes or negative control miRNA mimics (miR-NC). ** P < 0.01 versus miR-NC. c RT-qPCR was used to quantify HGF expression at mRNA levels. ** P < 0.01 versus negative control. d Western blot was used to measure HGF protein expression in A549 cells co-transfected with miR-144-3p or controls. e Western blot was used to detect the expression of HGF protein, and RT-qPCR was used to detect expression of miR-144-3p. ** P < 0.01 versus miR-NC <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13019-022-01861-3'>35568918</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1062-13019_2022_1861_fig3_html.pngAnti-Hepatocyte growth factor HGF Antibody Picoband&reg;HGF expression levels in lung cancer tissues and various lung cancer lines. a Immunohistochemical staining was used to measure HGF protein expression. b RT-qPCR was used to quantify HGF expression at the mRNA level in tumor tissues and paired normal tissues. c RT-qPCR was used to analyze HGF expression in lung cancer cell lines and a normal lung cell line. ** P < 0.01 versus controls. Scale bar = 50 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13019-022-01861-3'>35568918</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1062-13019_2022_1861_fig4_html.pngAnti-Hepatocyte growth factor HGF Antibody Picoband&reg;Function of miR-144-3p in A549 cells. a Proliferation of A549 cells transfected with control or miR-144-3p mimics together with HGF was detected by CCK-8 assay. * P < 0.05 versus negative control miRNA mimics (miR-NC). ## P < 0.01 versus miR-144-3p + Vector. b Flow cytometry was used to analyze the cell cycle. * P < 0.05 versus miR-NC. ## P < 0.01 versus miR-144-3p + Vector. Representative images and counts of cell migration following transfection with control or miR-144-3p together with HGF, c Transwell assay and d Wound-healing assay. * P < 0.05 versus miR-NC. ## P < 0.01 versus miR-144-3p + Vector. Scale bar = 25 μm. miR-NC, miR-144, miR-144 + vector and miR-144 + HGF need to be explained in a figure <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13019-022-01861-3'>35568918</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1062-13019_2022_1861_fig5_html.pngAnti-Hepatocyte growth factor HGF Antibody Picoband&reg;Function of miR-144-3p in the phenotype of NCI-H1299 cells. a Proliferation of NCI-H1299 cells transfected with control or miR-144-3p mimics together with HGF, as determined by CCK-8 assay. ** P < 0.01 versus miR-NC. ## P < 0.01 versus miR-144-3p + Vector. b Flow cytometry was used to analyze the cell cycle. ** P < 0.01 versus miR-NC. ## P < 0.01 versus miR-144-3p + Vector. Represented images and counts of cell migration following transfection with control or miR-144-3p mimics together with HGF, as detected by c Transwell assay and d Wound-healing assay. ** P < 0.01 versus miR-NC. ## P < 0.01 versus miR-144-3p + Vector. Scale bar = 25 μm. miR-NC, miR-144, miR-144 + vector and miR-144 + HGF need to be explained in a figure <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13019-022-01861-3'>35568918</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1062-hgf-primary-antibodies-wb-testing-1.jpgAnti-Hepatocyte growth factor HGF Antibody Picoband&reg;Western blot analysis of HGF using anti-HGF antibody (RP1062). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human HUH7 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat RH35 whole cell lysates,<br> Lane 7: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGF antigen affinity purified polyclonal antibody (Catalog # RP1062) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HGF at approximately 70 kDa. The expected band size for HGF is at 83 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eaat2-antibody-rp1065-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1065-slc1a2-primary-antibodies-wb-testing-1.jpgAnti-EAAT2/GLT-1/SLC1A2 Antibody Picoband&reg; Western blot analysis of EAAT2/GLT-1/SLC1A2 using anti-EAAT2/GLT-1/SLC1A2 antibody (RP1065). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT2/GLT-1/SLC1A2 antigen affinity purified polyclonal antibody (Catalog # RP1065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT2/GLT-1/SLC1A2 at approximately 65 kDa. The expected band size for EAAT2/GLT-1/SLC1A2 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1065-slc1a2-primary-antibodies-ihc-testing-2.jpgAnti-EAAT2/GLT-1/SLC1A2 Antibody Picoband&reg; IHC analysis of EAAT2/GLT-1/SLC1A2 using anti-EAAT2/GLT-1/SLC1A2 antibody (RP1065). <br> EAAT2/GLT-1/SLC1A2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EAAT2/GLT-1/SLC1A2 Antibody (RP1065) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1065-slc1a2-primary-antibodies-ihc-testing-3.jpgAnti-EAAT2/GLT-1/SLC1A2 Antibody Picoband&reg; IHC analysis of EAAT2/GLT-1/SLC1A2 using anti-EAAT2/GLT-1/SLC1A2 antibody (RP1065). <br> EAAT2/GLT-1/SLC1A2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EAAT2/GLT-1/SLC1A2 Antibody (RP1065) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1065-slc1a2-primary-antibodies-if-testing-4.jpgAnti-EAAT2/GLT-1/SLC1A2 Antibody Picoband&reg; IF analysis of EAAT2/GLT-1/SLC1A2 using anti-EAAT2/GLT-1/SLC1A2 antibody (RP1065). <br> EAAT2/GLT-1/SLC1A2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EAAT2/GLT-1/SLC1A2 Antibody (RP1065) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1065-slc1a2-primary-antibodies-if-testing-5.jpgAnti-EAAT2/GLT-1/SLC1A2 Antibody Picoband&reg; IF analysis of EAAT2/GLT-1/SLC1A2 using anti-EAAT2/GLT-1/SLC1A2 antibody (RP1065). <br> EAAT2/GLT-1/SLC1A2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EAAT2/GLT-1/SLC1A2 Antibody (RP1065) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rbp4-picoband-trade-antibody-pb9360-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9360-rbp4-primary-antibodies-wb-testing-1.jpgAnti-RBP4 Antibody Picoband&reg;Western blot analysis of RBP4 using anti-RBP4 antibody (PB9360). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RBP4 antigen affinity purified polyclonal antibody (PB9360) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RBP4 at approximately 23 kDa. The expected band size for RBP4 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9360-rbp4-primary-antibodies-ihc-testing-2.jpgAnti-RBP4 Antibody Picoband&reg;IHC analysis of RBP4 using anti-RBP4 antibody (PB9360). <br> RBP4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RBP4 Antibody (PB9360) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9360-rbp4-primary-antibodies-if-testing-3.jpgAnti-RBP4 Antibody Picoband&reg;IF analysis of RBP4 using anti-RBP4 antibody (PB9360). <br> RBP4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RBP4 Antibody (PB9360) overnight at 4°C. Cy Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prolactin-picoband-trade-antibody-pb9361-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9361-1_1-WB-anti-prolactin-picoband-antibody.jpgAnti-Prolactin/PRL Antibody Picoband&reg;Anti-Prolactin Picoband antibody&#44; PB361&#44; Western blotting<br>All lanes: Anti Prolactin (PB9361) at 0.5ug/ml<br> WB: MCF-7 Whole Cell Lysate at 40ug<br> Predicted bind size: 25KD<br> Observed bind size: 25KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sfrp2-picoband-trade-antibody-pb9362-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9362-1-WB-anti-sfrp2-picoband-antibody.jpgAnti-SFRP2 Antibody Picoband&reg;Anti-SFRP2 Picoband antibody&#44; PB9362&#44; Western blotting<br>All lanes: Anti SFRP2 (PB9362) at 0.5ug/ml<br>Lane 1: COLO320 Whole Cell Lysate at 40ug<br>Lane 2: SW620 Whole Cell Lysate at 40ug<br>Predicted bind size: 33KD<br>Observed bind size: 33KD https://www.bosterbio.com/products/primary-antibodies/anti-bcrp-abcg2-picoband-trade-antibody-pb9364-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9364-abcg2-primary-antibodies-wb-testing-1.jpgAnti-BCRP/ABCG2 Antibody Picoband&reg;Western blot analysis of ABCG2 using anti-ABCG2 antibody (PB9364). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG2 antigen affinity purified polyclonal antibody (PB9364) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ABCG2 at approximately 75-80 kDa. The expected band size for ABCG2 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9364-abcg2-primary-antibodies-ihc-testing-1.jpgAnti-BCRP/ABCG2 Antibody Picoband&reg;IHC analysis of ABCG2 using anti-ABCG2 antibody (PB9364). <br>ABCG2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCG2 Antibody (PB9364) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9364-abcg2-primary-antibodies-ihc-testing-2.jpgAnti-BCRP/ABCG2 Antibody Picoband&reg;IHC analysis of ABCG2 using anti-ABCG2 antibody (PB9364). <br>ABCG2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABCG2 Antibody (PB9364) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc12a1-picoband-trade-antibody-pb9392-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9392-slc12a1-primary-antibodies-wb-testing-1.jpgAnti-SLC12A1 Antibody Picoband&reg; Western blot analysis of SLC12A1 using anti-SLC12A1 antibody (A01106). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: monkey kidney tissue lysates, <br> Lane 2: rat kidney tissue lysates, <br> Lane 3: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC12A1 antigen affinity purified polyclonal antibody (Catalog # A01106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC12A1 at approximately 150-290 kDa. The expected band size for SLC12A1 is at 121 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9392-slc12a1-primary-antibodies-ihc-testing-2.jpgAnti-SLC12A1 Antibody Picoband&reg; IHC analysis of SLC12A1 using anti-SLC12A1 antibody (PB9392). <br> SLC12A1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A1 Antibody (PB9392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9392-slc12a1-primary-antibodies-ihc-testing-3.jpgAnti-SLC12A1 Antibody Picoband&reg; IHC analysis of SLC12A1 using anti-SLC12A1 antibody (PB9392). <br> SLC12A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A1 Antibody (PB9392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9392-slc12a1-primary-antibodies-ihc-testing-4.jpgAnti-SLC12A1 Antibody Picoband&reg; IHC analysis of SLC12A1 using anti-SLC12A1 antibody (PB9392). <br> SLC12A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC12A1 Antibody (PB9392) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9392-slc12a1-primary-antibodies-if-testing-5.jpgAnti-SLC12A1 Antibody Picoband&reg; IF analysis of SLC12A1 using anti-SLC12A1 antibody (PB9392). <br> SLC12A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC12A1 Antibody (PB9392) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9392-slc12a1-primary-antibodies-if-testing-6.jpgAnti-SLC12A1 Antibody Picoband&reg; IF analysis of SLC12A1 using anti-SLC12A1 antibody (PB9392). <br> SLC12A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SLC12A1 Antibody (PB9392) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-shc-picoband-trade-antibody-pb9391-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9391-shc1-primary-antibodies-wb-testing-1.jpgAnti-SHC/SHC1 Antibody Picoband&reg;Western blot analysis of SHC1 using anti-SHC1 antibody (PB9391). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC1 antigen affinity purified polyclonal antibody (PB9391) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHC1 at approximately 63 kDa. The expected band size for SHC1 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc22a2-picoband-trade-antibody-pb9394-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9394-slc22a2-primary-antibodies-wb-testing-1_1.jpgAnti-SLC22A2 Antibody Picoband&reg;Western blot analysis of SLC22A2 using anti-SLC22A2 antibody (PB9394). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC22A2 antigen affinity purified polyclonal antibody (PB9394) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC22A2 at approximately 63 kDa. The expected band size for SLC22A2 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9394-slc22a2-primary-antibodies-ihc-testing-1.jpgAnti-SLC22A2 Antibody Picoband&reg;IHC analysis of SLC22A2 using anti-SLC22A2 antibody (PB9394). <br>SLC22A2 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A2 Antibody (PB9394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9394-slc22a2-primary-antibodies-ihc-testing-2.jpgAnti-SLC22A2 Antibody Picoband&reg;IHC analysis of SLC22A2 using anti-SLC22A2 antibody (PB9394). <br>SLC22A2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A2 Antibody (PB9394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9394-slc22a2-primary-antibodies-ihc-testing-3.jpgAnti-SLC22A2 Antibody Picoband&reg;IHC analysis of SLC22A2 using anti-SLC22A2 antibody (PB9394). <br>SLC22A2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC22A2 Antibody (PB9394) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd62p-picoband-trade-antibody-pb9363-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9363-cd62p-primary-antibodies-wb-testing-1.jpgAnti-CD62P/SELP Antibody Picoband&reg; Western blot analysis of CD62P using anti-CD62P antibody (PB9363). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD62P antigen affinity purified polyclonal antibody (PB9363) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD62P at approximately 140 kDa. The expected band size for CD62P is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9363-cd62p-primary-antibodies-ihc-testing-2.jpgAnti-CD62P/SELP Antibody Picoband&reg; IHC analysis of CD62P using anti-CD62P antibody (PB9363). <br> CD62P was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD62P Antibody (PB9363) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9363-cd62p-primary-antibodies-ihc-testing-3.jpgAnti-CD62P/SELP Antibody Picoband&reg; IHC analysis of CD62P using anti-CD62P antibody (PB9363). <br> CD62P was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD62P Antibody (PB9363) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9363-cd62p-primary-antibodies-ihc-testing-4.jpgAnti-CD62P/SELP Antibody Picoband&reg; IHC analysis of CD62P using anti-CD62P antibody (PB9363). <br> CD62P was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD62P Antibody (PB9363) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adrb2-picoband-trade-antibody-pb9365-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9365-1-WB-anti-adrb2-beta-2-adrenergic-r-picoband-antibody.jpgAnti-beta 2 Adrenergic Receptor/ADRB2 Antibody Picoband&reg;Anti-ADRB2 Picoband antibody&#44; PB9365&#44; Western blotting<br>All lanes: AntiADRB2 (PB9365) at 0.5ug/ml<br>WB: Rat Brain Tissue Lysate at 50ug<br>Predicted bind size: 47KD<br>Observed bind size: 47KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smad1-picoband-trade-antibody-pb9395-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9395-1-WB-anti-smad1-picoband-antibody.jpgAnti-SMAD1 Antibody Picoband&reg;Anti-SMAD1 Picoband antibody&#44; PB9395&#44; Western blotting<br>All lanes: Anti SMAD1 (PB9395) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug<br>Lane 5: 293T Whole Cell Lysate at 40ug<br>Lane 6: MCF-7 Whole Cell Lysate at 40ug<br>Lane 7: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 52KD<br>Observed bind size: 52KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9395-fphar-12-643489-g004.jpgAnti-SMAD1 Antibody Picoband&reg;Silibinin inhibited the growth and promoted the apoptosis of LA795 cells. (A) . Migration of LA795 cells in the presence of 0, 3, 10, 30, 100, and 300 μM silibinin assessed by wound healing assay ( n = 4). (B) . Statistical results of the percentage of wound area in (A) ( n = 4) (C) . Statistical results of LA795 cells viability incubated with different concentrations of silibinin ( n = 6). (D) . Expression of β -catenin, E-cadherin, N-cadherin, and vimentin of LA795 cells after treated with 200 μM silibinin for 24 h ( n = 3). (E) . Representative real-time images of LA795 cells incubated with different concentrations of silibinin ( n = 6). (F) . Representative images of apoptotic cells after 200 μM silibinin treatment for 24 h detected by annexin V apoptosis assay ( n = 3). (G) . Expression of cleaved-caspase 3 and cleaved-caspase 9 of LA795 cells after 200 μM silibinin treatment for 24 h ( n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.643489/full'>33935737</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aif-picoband-trade-antibody-pb9366-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9366-aif-primary-antibodies-wb-testing-1.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; Western blot analysis of AIF using anti-AIF antibody (PB9366). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: human Hela whole cell lysates,<br> Lane 6: human Jurkat whole cell lysates,<br> Lane 7: human 293T whole cell lysates,<br> Lane 8: human U251 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIF antigen affinity purified polyclonal antibody (Catalog # PB9366) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIF at approximately 67 kDa. The expected band size for AIF is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9366-aif-primary-antibodies-wb-testing-2.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; Western blot analysis of AIF using anti-AIF antibody (PB9366). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat stomach tissue lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: rat NRK whole cell lysates,<br> Lane 4: mouse stomach tissue lysates,<br> Lane 5: mouse kidney tissue lysates,<br> Lane 6: mouse pancreas tissue lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates,<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIF antigen affinity purified polyclonal antibody (Catalog # PB9366) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIF at approximately 67 kDa. The expected band size for AIF is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9366-3-IHC-anti-aif-picoband-antibody.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; IHC analysis of AIF using anti-AIF antibody (PB9366). <br> AIF was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIF Antibody (PB9366) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9366-4-IHC-anti-aif-picoband-antibody.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; IHC analysis of AIF using anti-AIF antibody (PB9366). <br> AIF was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIF Antibody (PB9366) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9366-5_1-IF-anti-aif-picoband-antibody.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; IHC analysis of AIF using anti-AIF antibody (PB9366).<br> AIF was detected in immunocytochemical section of SMMC-7721 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-AIF Antibody (PB9366) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9366-6-ihc-anti-aif-picoband-antibody.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; IHC analysis of AIF using anti-AIF antibody (PB9366). <br> AIF was detected in paraffin-embedded section of Mouse Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AIF Antibody (PB9366) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9366-7.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; IF analysis of AIF using anti-AIF antibody (PB9366). <br> AIF was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AIF Antibody (PB9366) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9366-8.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; IF analysis of AIF using anti-AIF antibody (PB9366). <br> AIF was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AIF Antibody (PB9366) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9366-9.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-AIF antibody (PB9366).<br> Overlay histogram showing Hela cells stained with PB9366 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIF Antibody (PB9366,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9366-aifm1-primary-antibodies-wb-testing-10.jpgAnti-AIF/AIFM1 Antibody Picoband&reg; Western blot analysis of AIF using anti-AIF antibody (PB9366). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: rat NRK whole cell lysates, <br> Lane 3: mouse stomach tissue lysates, <br> Lane 4: mouse kidney tissue lysates, <br> Lane 5: mouse pancreas tissue lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIF antigen affinity purified polyclonal antibody (PB9366) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for AIF at approximately 70 kDa. The expected band size for AIF is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smad3-picoband-trade-antibody-pb9396-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9396-smad3-primary-antibodies-wb-testing-1_1.jpgAnti-Smad3 Antibody Picoband&reg;Western blot analysis of Smad3 using anti-Smad3 antibody (PB9396). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat ovary tissue lysates,<br> Lane 7: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smad3 antigen affinity purified polyclonal antibody (PB9396) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Smad3 at approximately 50-55 kDa. The expected band size for Smad3 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9396-smad3-primary-antibodies-ihc-testing-1.jpgAnti-Smad3 Antibody Picoband&reg;IHC analysis of Smad3 using anti-Smad3 antibody (PB9396). <br>Smad3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Smad3 Antibody (PB9396) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9396-smad3-primary-antibodies-if-testing-1.jpgAnti-Smad3 Antibody Picoband&reg;IF analysis of Smad3 using anti-Smad3 antibody (PB9396) and anti-Tubulin Alpha antibody (M03989-3).<br> Smad3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Smad3 Antibody (PB9396) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-2-picoband-trade-antibody-pb9368-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9368-1-WB-anti-caspase-2-picoband-antibody.jpgAnti-Caspase-2/CASP2 Antibody Picoband&reg;Anti-Caspase-2 Picoband antibody&#44; PB9368&#44; Western blotting<br> All lanes: Anti Caspase-2 (PB9368) at 0.5ug/ml<br>Lane 1: Rat Lung Tissue Lysate at 50ug<br>Lane 2: Mouse Liver Tissue Lysate at 50ug<br>Lane 3: A549 Whole Cell Lysate at 40ug<br>Lane 4: PANC Whole Cell Lysate at 40ug<br>Lane 5: 293T Whole Cell Lysate at 40ug<br>Predicted bind size: 18KD<br>Observed bind size: 18KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caspase-7-picoband-trade-antibody-pb9369-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9369-1-WB-anti-caspase-7-picoband-antibody.jpgAnti-Caspase-7/CASP7 Antibody Picoband&reg;Anti-Caspase-7 Picoband antibody&#44; PB9369&#44; Western blotting<br>All lanes: Anti Caspase-7(PB9369) at 0.5ug/ml<br>Lane 1: A549 Whole Cell Lysate at 40ug<br>Lane 2: Rat Spleen Tissue Lysate at 50ug<br>Lane 3: Rat Lung Tissue Lysate at 50ug<br>Predicted bind size: 34KD<br>Observed bind size: 34KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9369-2-IHC-anti-caspase-7-picoband-antibody.jpgAnti-Caspase-7/CASP7 Antibody Picoband&reg;Anti-Caspase-7 Picoband antibody&#44; PB9369&#44;IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-d1-picoband-trade-antibody-pb9370-boster.html2026-03-24T05:04:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-ccnd1-primary-antibodies-wb-testing-1.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;Western blot analysis of CCND1 using anti-CCND1 antibody (PB9370). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: rat lung tissue lysates,<br> Lane 5: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCND1 antigen affinity purified polyclonal antibody (PB9370) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCND1 at approximately 34 kDa. The expected band size for CCND1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-ccnd1-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;IHC analysis of CCND1 using anti-CCND1 antibody (PB9370). <br> CCND1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCND1 Antibody (PB9370) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-ccnd1-primary-antibodies-ihc-testing-2.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;IHC analysis of CCND1 using anti-CCND1 antibody (PB9370). <br> CCND1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCND1 Antibody (PB9370) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-ccnd1-primary-antibodies-ihc-testing-3.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;IHC analysis of CCND1 using anti-CCND1 antibody (PB9370). <br> CCND1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCND1 Antibody (PB9370) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-ccnd1-primary-antibodies-if-testing-1.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;IF analysis of CCND1 using anti-CCND1 antibody (PB9370) and anti-Tubulin Alpha antibody (M03989-3).<br> CCND1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CCND1 Antibody (PB9370) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-ijcep0006-2460-f3.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;ZFX knockdown altered levels or activities of proteins related to proliferation, survival and motility. A: Western blot showing phosphorylated and total levels of STAT3, Akt and ERK1/2 in H1299 cells treated as indicated. B: Western blot showing levels of Cyclin D1, Cyclin B1, Ki-67, Bcl-2, MMP-2, c-Myc and Survivin in H1299 cells treated as indicated. C: Western blot showing phosphorylated levels of ATM in H1299, U87 and U251 cells treated as indicated. All experiments were repeated twice. GAPDH was used as the loading control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816815/'>24228108</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-12885_2014_article_4882_fig3_html.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;The effect of ADI on apoptosis-related proteins and cell cycle protein cyclin D1 in pancreatic cancer cells. A and B , Treatment with ADI (1 mU/mL) regulates the levels of antiapoptotic proteins XIAP and survivin, and pro-apoptotic proteins caspase-3 and caspase-9 in PANC-1 cells. *, P < 0.05 as compared with the control group (0 mU/mL ADI); NS, not significant. C , ADI does not alter the expression level of p53 and p21 proteins in PANC-1 cells, as compared with the control group. D and E , ADI does not alter the expression level of p53 and p21 proteins in BxPC-3 cells, as compared with the control group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1471-2407-14-686'>25240403</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-12885_2014_article_4882_fig4_html.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;The effect of ADI on caspase-3 and cyclin D1, and the phosphorylation of NF-κB p65, STAT3, Akt, and ERK1/2 in ASS-deficient PANC-1 cells. A , ADI (1 mU/mL) up-regulates caspase-3 protein and decreases cell cycle protein cyclin D1 in a time-dependent manner in PANC-1 cells. *, P < 0.05 as compared with the treatment at 0 h; NS, not significant. B , ADI treatment (1 mU/mL for 0–24 h) of PANC-1 cells resulted in reduced phosphorylation of the NF-κB p65 subunit at Ser536. *, P < 0.05 as compared with the treatment group at 0 h; NS, not significant. C , The effect of ADI (1 mU/mL) on the phosphorylation of cell survival- associated proteins STAT3, Akt, and ERK1/2. *, P < 0.05 as compared with the control group; NS, not significant. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1471-2407-14-686'>25240403</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-fphar-09-00674-g004.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;Shh and AKT signaling pathway inhibitors abolish the effect of EGCG on the growth of DPCs and ORSCs. (A,B) After treatment with EGCG and/or cyclopamine, GANT61 or LY294002, the cell viability of DPCs and ORSCs was assessed by MTT assay. (C,D) Protein levels of cyclinB1 and cyclinD1 in DPCs and ORSCs were assessed by western blot with β-actin as the internal reference. All experiments were repeated three times. The results are presented as mean ± SD. ### p < 0.001 compared with the control group; ∗∗∗ p < 0.001 compared with the EGCG group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00674/full'>29997505</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-fphar-09-00674-g001.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;Epigallocatechin-3-gallate (EGCG) accelerates the growth of DPCs and ORSCs. (A,B) After treatment with 0.25, 0.5, 1, 2, and 4 μM EGCG for 12, 24, and 48 h, the cell viability of DPCs and ORSCs was assessed by MTT assay. $ p < 0.05 for 12 h, ∗∗∗ p < 0.001 for 24 h, ### p < 0.001 for 48 h, all compared with the control group. (C) After treatment with different concentrations of EGCG, the protein level of cyclinB1 in DPCs and ORSCs was detected by western blot. β-actin served as the internal reference. (D) Western blot was performed to detect the protein level of cyclinD1 in DPCs and ORSCs after treatment with EGCG. All experiments were repeated three times. The results are presented as mean ± SD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00674/full'>29997505</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-ijbsv15p0533g004.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;Interfering RRM2 inhibits the proliferation of cancer cells. A and B Western blot analysis showed that cyclin A, cyclin B1, and cyclin D1 were down-regulated in RRM2-transfected U87 cells. data expressed as mean ± SD of three independent experiments, # P <0.001 and Ф P <0.001, versus shNC. C RRM2 depletion significantly increased the proportion of U87 cells in G2 and M phase using flow cytometry. D and E Cell proliferation was detected by CCK-8 assay in U87 and LN229 cells; data expressed as mean ± SD of three independent experiments, # P < 0.001 and Ф P < 0.001, versus shNC. F and G Silencing of RRM2 inhibited the growth of U87 cells analyzed by colony-forming assay. The colonies were calculated. data expressed as mean ± SD of three independent experiments, # P < 0.001 and Ф P < 0.001, versus shNC.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367584/'>30745840</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-fonc-11-637298-g002.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;KDM2B restrains cell proliferation in CRC and induces DNA damage. (A) KDM2B protein was analyzed by Western blotting in the indicated normal human colon epithelial cells (CCD841CoN) and CRC cell lines (HT-29, ROK, LOVO, and DLD-1) * p < 0.05, ** p < 0.01, *** p < 0.001. (B,C) HT-29 and DLD-1 were transfected with siRNA (siKDM2B #1 and siKDM2B #2) and negative control (NC) using lipofectamine 2000. The relative expression of KDM2B mRNA was examined by real-time qRT-PCR after normalizing to GAPDH ( n = 3), *** p < 0.001. (D,E) The assessment of the expression level of KDM2B protein and the bar chart of quantified KDM2B protein expression in transfected HT-29 and DLD-1 cells. Data are presented as Mean ± SEM ( n = 3). Statistical significant differences in mRNA and protein in cells were observed (* p < 0.05, ** p < 0.01, *** p < 0.001 vs . negative control). The results of the CCK-8 assay (F,G) demonstrated that cell viability decreased in HT-29 and DLD-1 cells after KDM2B knockdown * p < 0.05, ** p < 0.01 vs . control. (H,I) HT-29 and DLD-1 cell colonies formed and graphical presentation of the average of colonies formed in control (NC) and transfected groups (#1 and #2). Data are expressed as Mean ± SEM ( n = 3). ** p < 0.01. (J) Comet images from HT-29 and DLD-1 cells after knockdown of KDM2B. The transfected cell group shows increasing levels of damage compared with the negative control. The number of cells scored in each measured concentration was 50. (K) The bar chart of the mean tail comet in percentage *** p < 0.001. (L) Representative densities of P21, P27, Cyclin D, and β-Tubulin proteins after Western blot experiment. (M) Cluster bar charts of representative proteins ** p < 0.01, *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fonc.2021.637298/full'>33791221</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9370-fonc-11-648985-g001.jpgAnti-Cyclin D1/CCND1 Antibody Picoband&reg;PD promotes the anti-tumor effects of sorafenib in PC3 cells. (A) . The effects of PD, sorafenib and PD plus sorafenib on cell viability. (B, C) . The changes in the cell membrane and nucleus after treatment with PD alone, sorafenib (Sor) alone or PD plus sorafenib. (D) . The induction of apoptosis by PD, Sor and PD + Sor. (E) . The protein expression levels of Caspase 3, C-Caspase 3, PARP and C-PARP were examined after cells were treated with 10 μM PD alone, 10 μM sorafenib alone or PD plus sorafenib for 6 h. (F) . The ability of cells to achieve colony growth was assessed after treatment with PD alone, sorafenib alone or PD plus sorafenib for 10 days. (G) . The proliferation of cells was monitored using the CFDA SE assay after treatment with PD alone, sorafenib alone or PD plus sorafenib for 5 days. (H, I) . The cell cycle distribution of PC3 cells following treatment with PD alone, sorafenib alone or PD plus sorafenib for 24 h after pre-treatment with (H) or without (I) 2 mM thymidine. (J) . Changes in the protein expression levels of CDK4, CDK6 and cyclin D1 after treatment with 5 μM PD alone, 2.5 μM sorafenib alone or PD plus sorafenib for 24 h. * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fonc.2021.648985/full'>34026624</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smad4-picoband-trade-antibody-pb9397-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9397-1-WB-anti-smad4-picoband-antibody.jpgAnti-Smad4 Antibody Picoband&reg;Anti-SMAD4 Picoband antibody&#44; PB9397&#44; Western blotting<br>All lanes: Anti SMAD4 (PB9397) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Lane 3: Rat Skeletal Muscle Tissue Lysate at 50ug<br>Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50ug<br>Lane 5: U87 Whole Cell Lysate at 40ug<br>Lane 6: Human Placenta Tissue Lysate at 50ug<br>Lane 7: HT1080 Whole Cell Lysate at 40ug<br>Lane 8: HELA Whole Cell Lysate at 40ug<br>Lane 9: NEURO Whole Cell Lysate at 40ug<br>Predicted bind size: 60KD<br>Observed bind size: 60KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smn1-2-picoband-trade-antibody-pb9398-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9398-smn-primary-antibodies-wb-testing-1.jpgAnti-SMN1/2 Antibody Picoband&reg;Western blot analysis of SMN1/2 using anti-SMN1/2 antibody (PB9398). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human A375 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human Raji whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMN1/2 antigen affinity purified polyclonal antibody (PB9398) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SMN1/2 at approximately 39 kDa. The expected band size for SMN1/2 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9398-4-IHC-anti-smn1-2-picoband-antibody.jpgAnti-SMN1/2 Antibody Picoband&reg;Anti-SMN1/2 Picoband antibody&#44; PB9398&#44;IHC(P)<br>IHC(P): Human Mammary Cancer Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9398-3-IHC-anti-smn1-2-picoband-antibody.jpgAnti-SMN1/2 Antibody Picoband&reg;Anti-SMN1/2 Picoband antibody&#44; PB9398&#44;IHC(P)<br>IHC(P): Rat Brain Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9398-2-IHC-anti-smn1-2-picoband-antibody.jpgAnti-SMN1/2 Antibody Picoband&reg;Anti-SMN1/2 Picoband antibody&#44; PB9398&#44;IHC(P)<br>IHC(P): Mouse Brain Tissue https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9398-5_1.jpgAnti-SMN1/2 Antibody Picoband&reg; IF analysis of SMN1/2 using anti-SMN1/2 antibody (PB9398). <br> SMN1/2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SMN1/2 Antibody (PB9398) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9398-6.jpgAnti-SMN1/2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-SMN1/2 antibody (PB9398).<br>Overlay histogram showing A431 cells stained with PB9398 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMN1/2 Antibody (PB9398,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-frzb-picoband-trade-antibody-pb9372-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9372-1-WB-anti-frzb-sfrp-3-picoband-antibody.jpgAnti-FRZB Antibody Picoband&reg;Anti-FRZB Picoband antibody&#44; PB9372&#44; Western blotting<br>All lanes: Anti FRZB (PB9372) at 0.5ug/ml<br>Lane 1: PANC Whole Cell Lysate at 40ug<br>Lane 2: U87 Whole Cell Lysate at 40ug<br>Predicted bind size: 36KD<br>Observed bind size: 36KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmdar2b-picoband-trade-antibody-pb9373-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9373-nmdar2b-primary-antibodies-wb-testing-1.jpgAnti-NMDAR2B/GRIN2B Antibody Picoband&reg; Western blot analysis of NMDAR2B using anti-NMDAR2B antibody (PB9373). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat C6 whole cell lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMDAR2B antigen affinity purified polyclonal antibody (Catalog # PB9373) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMDAR2B at approximately 180-190 kDa. The expected band size for NMDAR2B is at 166 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmdar2c-picoband-trade-antibody-pb9374-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9374-1-WB-anti-nmdar2c-picoband-antibody.jpgAnti-NMDAR2C/GRIN2C Antibody Picoband&reg;Anti-NMDAR2C Picoband antibody&#44; PB9374&#44; Western blotting<br>All lanes: Anti NMDAR2C (PB9374) at 0.5ug/ml<br>WB: Rat Brain Tissue Lysate at 50ug<br>Predicted bind size: 134KD<br>Observed bind size: 134KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hkdc1-picoband-trade-antibody-pb9375-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9375-hkdc1-primary-antibodies-wb-testing-1.jpgAnti-HKDC1 Antibody Picoband&reg; Western blot analysis of HKDC1 using anti-HKDC1 antibody (PB9375). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HKDC1 antigen affinity purified polyclonal antibody (Catalog # PB9375) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HKDC1 at approximately 103 kDa. The expected band size for HKDC1 is at 103 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hla-a-picoband-trade-antibody-pb9376-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9376-hla-a-primary-antibodies-wb-testing-1.jpgAnti-HLA A/HLA-A Antibody Picoband&reg; Western blot analysis of HLA A using anti-HLA A antibody (PB9376). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human HL-60 whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human HEL whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA A antigen affinity purified polyclonal antibody (Catalog # PB9376) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA A at approximately 41 kDa. The expected band size for HLA A is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9376-3-IHC-anti-hla-a-picoband-antibody.jpgAnti-HLA A/HLA-A Antibody Picoband&reg; IHC analysis of HLA A using anti-HLA A antibody (PB9376). <br> HLA A was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HLA A Antibody (PB9376) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9376-fonc-13-1089090-g004.jpgAnti-HLA A/HLA-A Antibody Picoband&reg;Signaling pathways and genes altered in CRC progression. (A) Changes in signaling pathways as CRC developed in sample S115 by GSVA analysis. (B) Spatial distribution and expression trend of ribosome pathway in S115. (C) RPL5 (left) and IMP3 (right) expression gradually increased in the direction of the red arrow. (D) Spatial distribution and expression trend of antigen processing and presentation pathway in S115. (E) HLA-A (left) and HSPA2 (right) expression gradually increased in the direction of the blue arrow. (F, G) Expression direction of peroxisome pathway (F) and leucine and isoleucine degradation pathway (G) in S115. (H, I) Immunohistochemical staining showed expression of RPL5 (H) and HLA-A (I) in Mucosa and cancer(up) and Invasive margin(down) (n=45). The scale bars on the lower right in (H) , (I) are 100 µm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9928961/&orig_host=www.ncbi.nlm.nih.gov'>36816947</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hla-c-picoband-trade-antibody-pb9377-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9377-hla-c-primary-antibodies-wb-testing-1_1.jpgAnti-HLA-C Antibody Picoband&reg; Western blot analysis of HLA-C using anti-HLA-C antibody (PB9377). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human U20S whole cell lysates,<br> Lane 5: human Hela whole cell lysates,<br> Lane 6: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-C antigen affinity purified polyclonal antibody (Catalog # PB9377) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-C at approximately 41 kDa. The expected band size for HLA-C is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9377-hla-c-primary-antibodies-ihc-testing-2.jpgAnti-HLA-C Antibody Picoband&reg;Figure 2 IHC analysis of HLA-C using anti-HLA-C antibody (PB9377).<br>HLA-C was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HLA-C Antibody (PB9377) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9377-hla-c-primary-antibodies-icc-testing-3.jpgAnti-HLA-C Antibody Picoband&reg; IHC analysis of HLA-C using anti-HLA-C antibody (PB9377).<br> HLA-C was detected in immunocytochemical section of HELA Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-HLA-C Antibody (PB9377) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9377-hla-c-primary-antibodies-if-testing-4.jpgAnti-HLA-C Antibody Picoband&reg; IF analysis of HLA-C using anti-HLA-C antibody (PB9377) <br> HLA-C was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-HLA-C Antibody (PB9377) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9377-hla-c-primary-antibodies-if-testing-5.jpgAnti-HLA-C Antibody Picoband&reg; IF analysis of HLA-C using anti-HLA-C antibody (PB9377) <br> HLA-C was detected in paraffin-embedded section of human oesophagus squama cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-HLA-C Antibody (PB9377) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9377-hla-c-primary-antibodies-fc-testing-6.jpgAnti-HLA-C Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-HLA-C antibody (PB9377). <br>Overlay histogram showing SiHa cells stained with PB9377 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLA-C Antibody (PB9377&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkm2-picoband-trade-antibody-pb9379-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-99188-g002.jpgAnti-PKM2 Antibody Picoband&reg;Effects of hypoxia on SLC16A8 expression and metabolic reprogramming of colorectal cancer cells. A: The expression level of SLC16A8 in colorectal cancer cell lines; B: The expression characteristics of SLC16A8 under hypoxia; C: The effect of hypoxia on the ECAR of RKO and LoVo; D: The effect of hypoxia on the extracellular lactate levels of RKO and LoVo; E: The effect of hypoxia on the expression of PKM2 and LDHA, the key proteins of metabolic reprogramming; F: The effect of hypoxia on glucose consumption in RKO and LoVo cells. a P < 0.05, b P < 0.01, c P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-99188-g005.jpgAnti-PKM2 Antibody Picoband&reg;SLC16A8 siRNA reverses the effects of hypoxia on cell metabolic reprogramming. A: SLC16A8 siRNA affects the expression of SLC16A8 in hypoxia treated cells; B: Effect of SLC16A8 siRNA on ECAR of hypoxia treated cells; C: SLC16A8 siRNA can affect the level of extracellular lactic acid after hypoxia treatment; D: SLC16A8 siRNA affects the expression of PKM2 and LDHA in hypoxia treated cells; E: Effect of SLC16A8 siRNA on glucose consumption in hypoxia treated cells. a P < 0.05, b P < 0.01, c P < 0.001. 2-DG: 2-deoxyglucose.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-pkm2-primary-antibodies-wb-testing-1.jpgAnti-PKM2 Antibody Picoband&reg; Western blot analysis of PKM2 using anti-PKM2 antibody (PB9379). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human MDA-B-453 whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human U87 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat L6 whole cell lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKM2 antigen affinity purified polyclonal antibody (Catalog # PB9379) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKM2 at approximately 58 kDa. The expected band size for PKM2 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-pkm2-primary-antibodies-ihc-testing-2.jpgAnti-PKM2 Antibody Picoband&reg; IHC analysis of PKM2 using anti-PKM2 antibody (PB9379). <br> PKM2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKM2 Antibody (PB9379) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-pkm2-primary-antibodies-ihc-testing-3.jpgAnti-PKM2 Antibody Picoband&reg; IHC analysis of PKM2 using anti-PKM2 antibody (PB9379). <br> PKM2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKM2 Antibody (PB9379) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-pkm2-primary-antibodies-ihc-testing-4.jpgAnti-PKM2 Antibody Picoband&reg; IHC analysis of PKM2 using anti-PKM2 antibody (PB9379). <br> PKM2 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKM2 Antibody (PB9379) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-pkm2-primary-antibodies-ihc-testing-5.jpgAnti-PKM2 Antibody Picoband&reg; IHC analysis of PKM2 using anti-PKM2 antibody (PB9379). <br> PKM2 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PKM2 Antibody (PB9379) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9379-pkm2-primary-antibodies-if-testing-6.jpgAnti-PKM2 Antibody Picoband&reg; IF analysis of PKM2 using anti-PKM2 antibody (PB9379). <br> PKM2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PKM2 Antibody (PB9379) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pld1-picoband-trade-antibody-pb9380-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9380-1-WB-anti-pld1-phospholipase-d1-picoband-antibody.jpgAnti-Phospholipase D1/PLD1 Antibody Picoband&reg;Anti-PLD1 Picoband antibody&#44; PB9380&#44; Western blotting<br>All lanes: Anti PLD1 (PB9380) at 0.5ug/ml<br>Lane 1: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: 22RV1 Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Predicted bind size: 124KD<br>Observed bind size: 124KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pln-picoband-trade-antibody-pb9381-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9381-pln-primary-antibodies-wb-testing-1.jpgAnti-Phospholamban/PLN Antibody Picoband&reg; Western blot analysis of PLN using anti-PLN antibody (PB9381). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates, <br> Lane 2: rat heart tissue lysates, <br> Lane 3: mouse heart tissue lysates, <br> Lane 4: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLN antigen affinity purified polyclonal antibody (Catalog # PB9381) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected respectively for PLN at approximately 24 kDa and 12 kDa. The expected band size for PLN is at 6 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9381-pln-primary-antibodies-ihc-testing-2.jpgAnti-Phospholamban/PLN Antibody Picoband&reg; IHC analysis of PLN using anti-PLN antibody (PB9381). <br> PLN was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLN Antibody (PB9381) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9381-pln-primary-antibodies-ihc-testing-3.jpgAnti-Phospholamban/PLN Antibody Picoband&reg; IHC analysis of PLN using anti-PLN antibody (PB9381). <br> PLN was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PLN Antibody (PB9381) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calcineurin-a-picoband-trade-antibody-pb9382-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9382-1-WB-anti-calcineurin-a-picoband-antibody.jpgAnti-Calcineurin A/PPP3CA Antibody Picoband&reg;Anti-Calcineurin A Picoband antibody&#44; PB9382&#44; Western blotting<br>All lanes: Anti Calcineurin A (PB9382) at 0.5ug/ml<br>Lane 1: HELA Whole Cell Lysate at 40ug<br>Lane 2: A549 Whole Cell Lysate at 40ug<br>Lane 3: COLO320 Whole Cell Lysate at 40ug<br>Predicted bind size: 59KD<br>Observed bind size: 84KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-4-picoband-trade-antibody-pb9383-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9383-4-IHC-anti-peroxiredoxin-4-picoband-antibody.jpgAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg;Anti-Peroxiredoxin 4 Picoband antibody&#44; PB9383&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9383-peroxiredoxin-4-primary-antibodies-wb-testing-1.jpgAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg; Western blot analysis of Peroxiredoxin 4 using anti-Peroxiredoxin 4 antibody (PB9383). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 4 antigen affinity purified polyclonal antibody (Catalog # PB9383) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 4 at approximately 28-30 kDa. The expected band size for Peroxiredoxin 4 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9383-3-IHC-anti-peroxiredoxin-4-picoband-antibody.jpgAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg;Anti-Peroxiredoxin 4 Picoband antibody&#44; PB9383&#44; IHC(P)<br>IHC(P): Rat Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9383-2-IHC-anti-peroxiredoxin-4-picoband-antibody.jpgAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg;Anti-Peroxiredoxin 4 Picoband antibody&#44; PB9383&#44; IHC(P)<br>IHC(P): Mouse Brain Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9383-5-IF-anti-peroxiredoxin-4-picoband-antibody.jpgAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 4 using anti-Peroxiredoxin 4 antibody (PB9383).<br> Peroxiredoxin 4 was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 4 Antibody (PB9383) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9383-peroxiredoxin-4-primary-antibodies-if-testing-6.jpgAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg; IF analysis of Peroxiredoxin 4 using anti-Peroxiredoxin 4 antibody (PB9383). <br> Peroxiredoxin 4 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 4 Antibody (PB9383) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9383-peroxiredoxin-4-primary-antibodies-fcm-testing-7.pngAnti-Peroxiredoxin 4/PRDX4 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-Peroxiredoxin 4 antibody (PB9383).<br>Overlay histogram showing MCF-7 cells stained with PB9383 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 4 Antibody (PB9383,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peroxiredoxin-5-picoband-trade-antibody-pb9384-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9384-4-IHC-anti-peroxiredoxin-5-picoband-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg;Anti-Peroxiredoxin 5 Picoband antibody&#44; PB9384&#44; IHC(P)<br>IHC(P): Human Lung Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9384-3-IHC-anti-peroxiredoxin-5-picoband-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg;Anti-Peroxiredoxin 5 Picoband antibody&#44; PB9384&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9384-2-IHC-anti-peroxiredoxin-5-picoband-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg;Anti-Peroxiredoxin 5 Picoband antibody&#44; PB9384&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9384-1-WB-anti-peroxiredoxin-5-picoband-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg;Anti-Peroxiredoxin 5 Picoband antibody&#44; PB9384&#44; Western blotting<br>All lanes: Anti Peroxiredoxin 5 (PB9384) at 0.5ug/ml<br>Lane 1: A549 Whole Cell Lysate at 40ug<br>Lane 2: Rat Brain Tissue Lysate at 50ug<br>Lane 3: Mouse Brain Tissue Lysate at 50ug<br>Predicted bind size: 22KD<br>Observed bind size: 22KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9384-5-IF-anti-peroxiredoxin-5-picoband-antibody.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg; IHC analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (PB9384).<br> Peroxiredoxin 5 was detected in immunocytochemical section of SMMC Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Peroxiredoxin 5 Antibody (PB9384) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9384-6.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg; IF analysis of Peroxiredoxin 5 using anti-Peroxiredoxin 5 antibody (PB9384). <br> Peroxiredoxin 5 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Peroxiredoxin 5 Antibody (PB9384) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9384-7.jpgAnti-Peroxiredoxin 5/PRDX5 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-Peroxiredoxin 5 antibody (PB9384).<br>Overlay histogram showing A549 cells stained with PB9384 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Peroxiredoxin 5 Antibody (PB9384,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pten-picoband-trade-antibody-pb9385-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9385-1-WB-anti-pten-picoband-antibody.jpgAnti-PTEN Antibody Picoband&reg;Anti-PTEN Picoband antibody&#44; PB9385&#44; Western blotting<br>All lanes: Anti PTEN (PB9385) at 0.5ug/ml<br>WB: Rat Brain Tissue Lysate at 50ug<br>Predicted bind size: 47KD<br>Observed bind size: 47KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9385-sci2022-9993393.005.jpgAnti-PTEN Antibody Picoband&reg;HOTTIP regulates EZH2 to inhibit PTEN expression. Note: (a) catRAPID predicted binding region of HOTTIP and EZH2. (b) FISH analyzed the localization of EZH2 and HOTTIP in IR-K562 cells. (c) RIP-PCR were used to test the interaction between EZH2 and HOTTIP. ∗ p < 0.05 vs. IgG. (d) RT-qPCR and Western blot were used to measure EZH2 mRNA and protein level after knocking down HOTTIP. (e) Western blot analysis was used to measure EZH2 in BM-MNCs of CML patients. Right panel, bar chart of protein densitometric analysis. ∗∗∗ p < 0.001. RT-qPCR was used to detect EZH2 level in BM-MNCs of CML patients. Normalized to GAPDH. ∗∗∗ p < 0.001 vs. NC. Western blot analysis was used to measure EZH2 protein level in IR-K562 and K562 cells. Right panel, bar chart of protein densitometric analysis. ∗∗∗ p < 0.001; RT-qPCR was used to detect EZH2 mRNA level in CML cell lines (K562 and IR-K562). Normalized to GAPDH. ∗∗∗ P < 0.001 vs. NC. (f) RT-qPCR and Western blot were used to detect EZH2 and PTEN mRNA and protein levels after transfecting with specific sh-con or sh-EZH2. ∗∗∗ P < 0.001 vs. DMSO.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9477575/&orig_host=www.ncbi.nlm.nih.gov'>36117724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9385-sci2022-9993393.007.jpgAnti-PTEN Antibody Picoband&reg;The effect of HOTTIP on CML cell in vivo. Note: (a) Tazemetostat drug structure. (b) Analysis of H3K27me3. PTEN expression in IR-K562 cells treated by Tazemetostat with different concentrations for 48 h. ∗∗∗ p < 0.001 vs. DMSO. (c) IR-K562 cells were engineered to stably knock down HOTTIP, and the cells were then subcutaneously injected into the nude mice to establish CML xenograft tumors. Tumor volumes were monitored by direct measurement. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ P < 0.001 vs.sh-con. Representative tumor sizes of xenograft mice in each group. The xenograft tumor wet weight in each group of mice. ∗ p < 0.05, ∗∗ P < 0.01 vs. sh-con. (d) Immunohistochemistry stain was used to measure the PTEN, c-caspase3 protein levels in xenograft tumors. (e) Western blot was used to detect the H3K27me3, PTEN, and c-caspase3 protein levels in xenograft tumors.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9477575/&orig_host=www.ncbi.nlm.nih.gov'>36117724</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9385-fig-3-1x.jpgAnti-PTEN Antibody Picoband&reg;miR-21 effect of on PTEN expression in CSCs. Cultured CSCs were treated with miR-21 mimics or its negative control scramble for 48 h, then cells were harvested and subjected to RT-PCR or Western blot. PTEN mRNA of Control, scramble treated or miR-21 mimics treated CSCs showed no significant difference (A), but PTEN protein dramatically decreased after miR-21 mimics treatment (B). a, P < 0.05 compared with Control; b, P < 0.05 compared with Scramble. n = 3 in each group. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/2859/'>28168101</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9385-fig-4-1x.jpgAnti-PTEN Antibody Picoband&reg;PTEN/PI3K/Akt pathway’s contribution in miR-21 induced proliferation in c-kit + CSCs. Cultured c-kit + CSCs were treated with miR-21 mimics for 48 h before subjected to EdU immunofluorescence (A–B), flow cytometry (C) or Western blot (D). To test the contribution of PTEN/PI3K/Akt signaling, PTEN and PI3K were inhibited with Phen or LY294002 respectively. (A) c-kit + CSCs were double stained by EdU (green) and DAPI (blue), and observed under a fluorescence microscope (Olympus). Bar = 50 µm. DAPI = propidium iodide. (B) The statistics of EdU positive CSCs from immunofluorescence in (A). n = 6 in each group. (C) Flow cytometry was employed to detect cell cycle profiles in CSCs underwent different treatments miR-21 mimics or Phen increased the proportion of S phase CSCs compared with Control or scramble treated groups. n = 3. (D) PTEN/PI3K/Akt pathway’s influences on BrdU expression, which was detected with immune blotting. Just like miR-21 mimics’ effect on BrdU, when PTEN was inhibited by Phen, there was notably increase of BrdU compared with Normal or Scramble group. When PI3K was inhibited by LY294002, there was notably decrease of BrdU in mimics+LY294002 group compared with mimics group in CSCs. n = 3 in each group. a, P < 0.05 compared with Control; b, P < 0.05 compared with Scramble; c, P < 0.05 compared with miR-21 mimics group; d, P < 0.05 compared with Phen group. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/2859/'>28168101</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9385-fig-5-1x.jpgAnti-PTEN Antibody Picoband&reg;Expression change of PTEN/PI3K/Akt pathway in the process of miR-21 mimics induced proliferation in c-kit + CSCs. Cultured CSCs were treated with miR-21 mimics for 48 h before the subsequent procedures. To test the contribution of PTEN/PI3K/Akt signaling to miR-21 mimics’s pro-proliferation effects in c-kit + CSCs, PTEN and PI3K were inhibited with Phen or LY294002 respectively. (A) RT-PCR was carried out to detect miR-21 mimics’s effects on PTEN expression at the mRNA level, which showed no change between Control, miR-21 scramble, miR-21 mimics and miR-21 mimics+ LY294002 group, while Phen resulted in a significant down-regulation of PTEN compared with the other groups. (B–C) Western blot was carried out to detect miR-21 mimics’s effects on PTEN protein expression, which showed that miR-21 mimics significantly down-regulated PTEN protein in miR-21 mimics group compared with the scramble group. In addition, both Phen treatment and miR-21 mimics incubation increased p-Akt level, while PI3K inhibitor LY294002 decreased p-Akt level dramatically ( P < 0.05). a, P < 0.05 compared with Control; b, P < 0.05 compared with miR-21 scramble group; c, P < 0.05 compared with miR-21 mimics group; d, P < 0.05 compared with Phen group. n = 3 in each group. p-Akt = phosphor-Akt. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/2859/'>28168101</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-socs2-picoband-trade-antibody-pb9400-boster.html2026-03-24T05:04:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9400-1-WB-anti-socs2-picoband-antibody.jpgAnti-SOCS2 Antibody Picoband&reg;Anti-Picoband antibody&#44; PB&#44; Western blotting<br>All lanes: Anti SOCS2 (PB9400) at 0.5ug/ml<br>Lane 1: PC-12 Whole Cell Lysate at 40ug<br>Lane 2: SW620 Whole Cell Lysate at 40ug<br>Lane 3: HELA Whole Cell Lysate at 40ug<br>Lane 4: MCF-7 Whole Cell Lysate at 40ug<br>Predicted bind size: 22KD<br>Observed bind size: 22KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ptger2-picoband-trade-antibody-pb9386-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9386-1_1-WB-anti-ptger2-picoband-antibody.jpgAnti-Prostaglandin E Receptor EP2/PTGER2 Antibody Picoband&reg;Anti-PTGER2 Picoband antibody&#44; PB9386&#44; Western blotting<br>All lanes: Anti PTGER2 (PB9386) at 0.5ug/ml<br> Lane 1: Human Placenta Tissue Lysate at 50ug<br> Lane 2: A549 Whole Cell Lysate at 40ug<br> Lane 3: HEPG2 Whole Cell Lysate at 40ug<br> Predicted bind size: 40KD<br> Observed bind size: 40KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sod1-picoband-trade-antibody-pb9402-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9402-sod1-primary-antibodies-wb-testing-1.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg;Western blot analysis of SOD1 using anti-SOD1 antibody (PB9402). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human T47D whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PB9402) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9402-sod1-primary-antibodies-ihc-testing-1.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg;IHC analysis of SOD1 using anti-SOD1 antibody (PB9402). <br> SOD1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOD1 Antibody (PB9402) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9402-sod1-primary-antibodies-fcm-testing-1.jpgAnti-Superoxide Dismutase 1/SOD1 Antibody Picoband&reg;Flow Cytometry analysis of MCF-7 cells using anti-SOD1 antibody (PB9402). <br> Overlay histogram showing MCF-7 cells stained with PB9402 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOD1 Antibody (PB9402, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rock2-picoband-trade-antibody-pb9387-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9387-rock2-primary-antibodies-wb-testing-1.jpgAnti-ROCK2 Antibody Picoband&reg; Western blot analysis of ROCK2 using anti-ROCK2 antibody (PB9387). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse brain tissue lysates, <br> Lane 4: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # PB9387) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161 kDa. The expected band size for ROCK2 is at 161 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9387-2_1-IHC-anti-rock2-picoband-antibody.jpgAnti-ROCK2 Antibody Picoband&reg; IHC analysis of ROCK2 using anti-ROCK2 antibody (PB9387). <br> ROCK2 was detected in a paraffin-embedded section of Mouse Cardiac Muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ROCK2 Antibody (PB9387) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9387-rock2-primary-antibodies-wb-testing-2.pngAnti-ROCK2 Antibody Picoband&reg; Western blot analysis of ROCK2 using anti-ROCK2 antibody (PB9387). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Normal group-rat colon tissue lysates, <br> Lane 2: Model group-rat colon tissue lysates, <br> Lane 3: Triditional Chinese medicine treatment (low dose)-rat colon tissue lysates, <br> Lane 4: Triditional Chinese medicine treatment (medium dose)-rat colon tissue lysates, <br> Lane 5: Triditional Chinese medicine treatment(high dose)-rat colon tissue lysates, <br> Lane 6: Western medicine treatment-rat colon tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified polyclonal antibody (Catalog # PB9387) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L)secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for ROCK2 at approximately 180 kDa. The expected band size for ROCK2 is at 161 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9387-3_1-IHC-anti-rock2-picoband-antibody.jpgAnti-ROCK2 Antibody Picoband&reg; IHC analysis of ROCK2 using anti-ROCK2 antibody (PB9387). <br> ROCK2 was detected in a paraffin-embedded section of Rat Brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ROCK2 Antibody (PB9387) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9387-4_1-IHC-anti-rock2-picoband-antibody.jpgAnti-ROCK2 Antibody Picoband&reg; IHC analysis of ROCK2 using anti-ROCK2 antibody (PB9387). <br> ROCK2 was detected in a paraffin-embedded section of Human Mammary Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ROCK2 Antibody (PB9387) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sox1-picoband-trade-antibody-pb9403-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9403-1-WB-anti-sox1-picoband-antibody.jpgAnti-SOX1 Antibody Picoband&reg;Anti-SOX1 Picoband antibody&#44; PB9403&#44; Western blotting<br>All lanes: Anti SOX1 (PB9403) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: NIH3T3 Whole Cell Lysate at 40ug<br>Predicted bind size: 39KD<br>Observed bind size: 39KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-syndecan-4-picoband-trade-antibody-pb9388-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9388-4-IHC-anti-syndecan-4-sdc4-antibody.jpgAnti-Syndecan 4/SDC4 Antibody Picoband&reg;Anti-Syndecan 4 Picoband antibody&#44; PB9388&#44; IHC(P)<br>IHC(P): Human Mammary Cancer Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9388-1-WB-anti-syndecan-4-sdc4-antibody.jpgAnti-Syndecan 4/SDC4 Antibody Picoband&reg;Anti-Syndecan 4 Picoband antibody&#44; PB9388&#44; Western blotting<br>All lanes: Anti Syndecan 4 (PB9388) at 0.5ug/ml<br>Lane 1: MCF-7 Whole Cell Lysate at 40ug<br>Lane 2: SW620 Whole Cell Lysate at 40ug<br>Lane 3: Rat Lung Tissue Lysate at 50ug<br>Predicted bind size: 22KD<br>Observed bind size: 22KD https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9388-3-IHC-anti-syndecan-4-sdc4-antibody.jpgAnti-Syndecan 4/SDC4 Antibody Picoband&reg;Anti-Syndecan 4 Picoband antibody&#44; PB9388&#44; IHC(P)<br>IHC(P): Rat Intestine Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9388-2-IHC-anti-syndecan-4-sdc4-antibody.jpgAnti-Syndecan 4/SDC4 Antibody Picoband&reg;Anti-Syndecan 4 Picoband antibody&#44; PB9388&#44; IHC(P)<br>IHC(P): Mouse Intestine Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-l-selectin-picoband-trade-antibody-pb9389-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9389-2-IHC-anti-l-selectin-antibody.jpgAnti-CD62L/SELL Antibody Picoband&reg;Anti-L-selectin Picoband antibody&#44; PB9389&#44; IHC(P)<br>IHC(P): Human Tonsil Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9389-1-WB-anti-l-selectin-antibody.jpgAnti-CD62L/SELL Antibody Picoband&reg;Anti-L-selectin Picoband antibody&#44; PB9389&#44; Western blotting<br>All lanes: Anti L-selectin (PB9389) at 0.5ug/ml<br>Lane 1: Human Placenta Tissue Lysate at 50ug<br>Lane 2: JURKAT Whole Cell Lysate at 40ug<br>Lane 3: CEM Whole Cell Lysate at 40ug<br>Lane 4: HL60 Whole Cell Lysate at 40ug<br>Lane 5: Rat Spleen Tissue Lysate at 50ug<br>Lane 6: Mouse Spleen Tissue Lysate at 50ug<br>Predicted bind size: 42KD<br>Observed bind size: 42KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sftpa1-2-picoband-trade-antibody-pb9390-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9390-sftpa1-2-primary-antibodies-wb-testing-1.jpgAnti-SFTPA1/2 Antibody Picoband&reg; Western blot analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates,<br> Lane 2: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1/2 antigen affinity purified polyclonal antibody (Catalog # PB9390) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1/2 at approximately 26-38 kDa. The expected band size for SFTPA1/2 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9390-2-IHC-anti-sftpa1-2-picoband-antibody.jpgAnti-SFTPA1/2 Antibody Picoband&reg; IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). <br> SFTPA1/2 was detected in paraffin-embedded section of Mouse Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1/2 Antibody (PB9390) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9390-3-IHC-anti-sftpa1-2-picoband-antibody.jpgAnti-SFTPA1/2 Antibody Picoband&reg; IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). <br> SFTPA1/2 was detected in paraffin-embedded section of Rat Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1/2 Antibody (PB9390) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9390-4-IHC-anti-sftpa1-2-picoband-antibody.jpgAnti-SFTPA1/2 Antibody Picoband&reg; IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). <br> SFTPA1/2 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1/2 Antibody (PB9390) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9390-5-IHC-anti-sftpa1-2-picoband-antibody.jpgAnti-SFTPA1/2 Antibody Picoband&reg; IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). <br> SFTPA1/2 was detected in frozen section of Mouse Lung Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1/2 Antibody (PB9390) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9390-6-IHC-anti-sftpa1-2-picoband-antibody.jpgAnti-SFTPA1/2 Antibody Picoband&reg; IHC analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). <br> SFTPA1/2 was detected in frozen section of Rat Lung Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1/2 Antibody (PB9390) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9390-sftpa1-2-primary-antibodies-if-testing-7.jpgAnti-SFTPA1/2 Antibody Picoband&reg; IF analysis of SFTPA1/2 using anti-SFTPA1/2 antibody (PB9390). <br> SFTPA1/2 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SFTPA1/2 Antibody (PB9390) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sf2-picoband-trade-antibody-pb9404-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9404-srsf1-primary-antibodies-wb-testing-1.jpgAnti-SF2/SRSF1 Antibody Picoband&reg; Western blot analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody (PB9404). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human SW620 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human 293T whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat liver tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF2/SRSF1 antigen affinity purified polyclonal antibody (Catalog # PB9404) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF2/SRSF1 at approximately 28 kDa. The expected band size for SF2/SRSF1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9404-srsf1-primary-antibodies-ihc-testing-2.jpgAnti-SF2/SRSF1 Antibody Picoband&reg; IHC analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody (PB9404). <br> SF2/SRSF1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF2/SRSF1 Antibody (PB9404) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9404-srsf1-primary-antibodies-ihc-testing-3.jpgAnti-SF2/SRSF1 Antibody Picoband&reg; IHC analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody (PB9404). <br> SF2/SRSF1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF2/SRSF1 Antibody (PB9404) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9404-srsf1-primary-antibodies-ihc-testing-4.jpgAnti-SF2/SRSF1 Antibody Picoband&reg; IHC analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody (PB9404). <br> SF2/SRSF1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SF2/SRSF1 Antibody (PB9404) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat6-picoband-trade-antibody-pb9405-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9405-1-WB-anti-stat6-picoband-antibody.jpgAnti-STAT6 Antibody Picoband&reg; Western blot analysis of STAT6 using anti-STAT6 antibody (PB9405). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT6 antigen affinity purified polyclonal antibody (Catalog # PB9405) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT6 at approximately 94 kDa. The expected band size for STAT6 is at 94 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stim1-picoband-trade-antibody-pb9406-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9406-stim1-primary-antibodies-wb-testing-1.jpgAnti-STIM1 Antibody Picoband&reg; Western blot analysis of STIM1 using anti-STIM1 antibody (PB9406, Left) and anti-STIM1 antibody (M00312, Right). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. <br> Lane 1: human Jurakt whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: rat liver tissue lysates,<br> Lane 5: rat RH35 whole cell lysates,<br> Lane 6: mouse liver tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody (Catalog # PB9406) and rabbit anti-STIM1 antigen affinity purified monoclonal antibody (Catalog # (M00312) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIM1 at approximately 97KD. The expected band size for STIM1 is at 77KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9406-2-IHC-anti-stim1-picoband-antibody.jpgAnti-STIM1 Antibody Picoband&reg; IHC analysis of STIM1 using anti-STIM1 antibody (PB9406). <br> STIM1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9406-3-IHC-anti-stim1-picoband-antibody.jpgAnti-STIM1 Antibody Picoband&reg; IHC analysis of STIM1 using anti-STIM1 antibody (PB9406). <br> STIM1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9406-4-IHC-anti-stim1-picoband-antibody.jpgAnti-STIM1 Antibody Picoband&reg; IHC analysis of STIM1 using anti-STIM1 antibody (PB9406). <br> STIM1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9406-stim1-primary-antibodies-if-testing-5.jpgAnti-STIM1 Antibody Picoband&reg; IF analysis of STIM1 using anti-STIM1 antibody (PB9406). <br> STIM1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-STIM1 Antibody (PB9406) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd36-picoband-trade-antibody-pb9371-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9371-2-IHC-anti-cd36-sr-b3-antibody.jpgAnti-CD36 Antibody Picoband&reg;Anti-CD36 Picoband antibody&#44; PB9371&#44; IHC(P)<br>IHC(P): Rat Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9371-1-WB-anti-cd36-sr-b3-antibody.jpgAnti-CD36 Antibody Picoband&reg;Anti-CD36 Picoband antibody&#44; PB9371&#44; Western blotting<br>All lanes: Anti CD36 (PB9371) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 3: Mouse Liver Tissue Lysate at 50ug<br>Lane 4: Mouse Cardiac Muscle Tissue Lysate at 50ug<br>Lane 5: SMMC Whole Cell Lysate at 40ug<br>Predicted bind size: 53KD<br>Observed bind size: 88KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9371-3.jpgAnti-CD36 Antibody Picoband&reg; IHC analysis of Galectin 1 using anti-Galectin 1 antibody (PB9371). <br> Galectin 1 was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Galectin 1 Antibody (PB9371) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-unrip-picoband-trade-antibody-pb9407-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9407-1_1.jpgAnti-Unrip/STRAP Antibody Picoband&reg; Western blot analysis of Unrip using anti-Unrip antibody (PB9407).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates<br> Lane 2: human Jurkat whole cell lysates<br> Lane 3: human PC-3 whole cell lysates<br> Lane 4: human THP-1 whole cell lysates<br> Lane 5: rat brain tissue lysates<br> Lane 6: mouse HEPA1-6 whole cell lysates<br> Lane 7: mouse RAW246.7 whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Unrip antigen affinity purified polyclonal antibody (Catalog # PB9407) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Unrip at approximately 39KD. The expected band size for Unrip is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9407-2-IHC-anti-unrip-picoband-antibody.jpgAnti-Unrip/STRAP Antibody Picoband&reg; IHC analysis of Unrip using anti-Unrip antibody (PB9407). <br> Unrip was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Unrip Antibody (PB9407) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-syntaxin-1a-picoband-trade-antibody-pb9408-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9408-1-WB-anti-syntaxin-1a-picoband-antibody.jpgAnti-Syntaxin 1a/STX1A Antibody Picoband&reg; Western blot analysis of Syntaxin 1a using anti-Syntaxin 1a antibody (PB9408). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: U87 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 1a antigen affinity purified polyclonal antibody (Catalog # PB9408) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 1a at approximately 37 kDa. The expected band size for Syntaxin 1a is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-synaptophysin-picoband-trade-antibody-pb9409-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9409-1_1.jpgAnti-Synaptophysin/SYP Antibody Picoband&reg; Western blot analysis of Synaptophysin using anti-Synaptophysin antibody (PB9409). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin antigen affinity purified polyclonal antibody (Catalog # PB9409) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptophysin at approximately 38KD. The expected band size for Synaptophysin is at 34KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prolactin-picoband-trade-antibody-pb9411-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9411-1-WB-anti-prolactin-picoband-antibody.jpgAnti-Prolactin/PRL Antibody Picoband&reg; Western blot analysis of Prolactin using anti-Prolactin antibody (PB9411). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Recombinant Human Resistin Protein 0.5ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolactin antigen affinity purified polyclonal antibody (Catalog # PB9411) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolactin at approximately 10 kDa. The expected band size for Prolactin is at 10 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-resistin-picoband-trade-antibody-pb9412-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9412-1-WB-anti-resistin-picoband-antibody.jpgAnti-Resistin/RETN Antibody Picoband&reg; Western blot analysis of Resistin using anti-Resistin antibody (PB9412). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Recombinant Human Resistin Protein 0.5ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Resistin antigen affinity purified polyclonal antibody (Catalog # PB9412) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Resistin at approximately 11 kDa. The expected band size for Resistin is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9412-antioxidants-13-01496-g005.pngAnti-Resistin/RETN Antibody Picoband&reg;Monomethyl fumarate (MMF) fully suppresses differentiation mixture (MIX)-induced protein expression of the transcription factors peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBP-α) (a) as well as the adipokines, resistin and adiponectin (b). Cellular protein abundance was assessed by Western blotting. Data are expressed as the means ± standard deviation; * p < 0.05 and ** p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.mdpi.com/2076-3921/13/12/1496'>39765824</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rank-picoband-trade-antibody-pb9413-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9413-1-WB-anti-rank-picoband-antibody.jpgAnti-RANK/TNFRSF11A Antibody Picoband&reg; Western blot analysis of RANK using anti-RANK antibody (PB9413). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HEPA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANK antigen affinity purified polyclonal antibody (Catalog # PB9413) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANK at approximately 100 kDa. The expected band size for RANK is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mafa-antibody-rp1066-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1066-1_1.jpgAnti-Transcription factor MafA MAFA Antibody Picoband&reg;Western blot analysis of MAFA using anti-MAFAantibody (RP1066).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human 22RV1 whole cell lysates, <br> Lane 4: rat PC-12 whole cell lysates,<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAFA antigen affinity purified polyclonal antibody (Catalog # RP1066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAFA at approximately 37KD. The expected band size for MAFA is at 37KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sfrp4-antibody-rp1067-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1067-1-WB-anti-sfrp4-antibody.jpgAnti-SFRP4 Antibody Picoband&reg;Anti-SFRP4 Picoband antibody&#44; RP1067&#44; Western blotting<br>All lanes: Anti SFRP4 (RP1067) at 0.5ug/ml<br>Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug<br>Lane 2: SKOV Whole Cell Lysate at 40ug<br>Predicted bind size: 40KD<br>Observed bind size: 40KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-synaptopodin-antibody-rp1069-boster.html2026-03-24T05:04:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1069-1-WB-anti-synaptopodin-antibody.jpgAnti-Synaptopodin/SYNPO Antibody Picoband&reg;Anti-Synaptopodin antibody&#44; RP1069&#44; Western blotting<br>All lanes: Anti Synaptopodin (RP1069) at 0.5ug/ml<br>Lane 1: Mosue Brain Tissue Lysate at 50ug<br>Lane 2: U87 Whole Cell Lysate at 40ug<br>Lane 3: HEPG2 Whole Cell Lysate at 40ug<br>Lane 4: 293T Whole Cell Lysate at 40ug<br>Predicted bind size: 99KD<br>Observed bind size: 99KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcg5-picoband-trade-antibody-pb9415-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9415-1-WB-anti-abcg5-picoband-antibody.jpgAnti-ABCG5 Antibody Picoband&reg; Western blot analysis of ABCG5 using anti-ABCG5 antibody (PB9415). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 2: A549 Whole Cell Lysate at 40ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody (Catalog # PB9415) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73 kDa. The expected band size for ABCG5 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9415-10020_2022_450_fig7_html.pngAnti-ABCG5 Antibody Picoband&reg;Quantitative real-time PCR and Western blot analysis of SR-BI, ABCG5, and ABCG8. A Quantitative real-time PCR analysis of the mRNA levels of SR-BI, ABCG8 and ABCG5 in liver of the mice in the control and LOX-1 groups. The expression of mRNA was normalized to that of GAPDH. B - E Western blot analysis of the hepatic lipid metabolism core pathway-related proteins SR-BI, ABCG5, and ABCG8 8 weeks after virus transduction. Values are expressed as mean ± SD. **P < 0.01 compared with the control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-022-00450-3'>35236285</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcb11-picoband-trade-antibody-pb9414-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9414-abcb11-primary-antibodies-wb-testing-1.jpgAnti-ABCB11 Antibody Picoband&reg; Western blot analysis of ABCB11 using anti-ABCB11 antibody (PB9414). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HCCT tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB11 antigen affinity purified polyclonal antibody (Catalog # PB9414) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB11 at approximately 146 kDa. The expected band size for ABCB11 is at 146 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9414-abcb11-primary-antibodies-ihc-testing-5.jpgAnti-ABCB11 Antibody Picoband&reg;IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414). <br>BSEP/ABCB11 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BSEP/ABCB11 Antibody (PB9414) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9414-abcb11-primary-antibodies-ihc-testing-6.jpgAnti-ABCB11 Antibody Picoband&reg;IHC analysis of BSEP/ABCB11 using anti-BSEP/ABCB11 antibody (PB9414). <br>BSEP/ABCB11 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BSEP/ABCB11 Antibody (PB9414) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9414-4-IHC-anti-abcb11-bsep-picoband-antibody.jpgAnti-ABCB11 Antibody Picoband&reg; IHC analysis of ABCB11 using anti-ABCB11 antibody (PB9414).<br> ABCB11 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ABCB11 Antibody (PB9414) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9414-3-IHC-anti-abcb11-bsep-picoband-antibody.jpgAnti-ABCB11 Antibody Picoband&reg; IHC analysis of ABCB11 using anti-ABCB11 antibody (PB9414).<br> ABCB11 was detected in paraffin-embedded section of Rat Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ABCB11 Antibody (PB9414) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9414-2-IHC-anti-abcb11-bsep-picoband-antibody.jpgAnti-ABCB11 Antibody Picoband&reg; IHC analysis of ABCB11 using anti-ABCB11 antibody (PB9414).<br> ABCB11 was detected in paraffin-embedded section of Human Liver Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ABCB11 Antibody (PB9414) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9414-5.jpgAnti-ABCB11 Antibody Picoband&reg; IF analysis of ABCB11 using anti-ABCB11 antibody (PB9414)<br> ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ABCB11 Antibody (PB9414) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abi1-picoband-trade-antibody-pb9416-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9416-abi1-primary-antibodies-ihc-testing-4.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg;IHC analysis of ABI1 using anti-ABI1 antibody (PB9416). <br>ABI1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABI1 Antibody (PB9416) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9416-abi1-primary-antibodies-fcm-testing-5.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-SSH3BP1/ABI1 antibody (PB9416). <br>Overlay histogram showing K562 cells stained with PB9416 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3BP1/ABI1 Antibody (PB9416, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9416-abi1-primary-antibodies-wb-testing-1.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; Western blot analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody (PB9416). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human U87 whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3BP1/ABI1 antigen affinity purified polyclonal antibody (Catalog # PB9416) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH3BP1/ABI1 at approximately 65 kDa. The expected band size for SSH3BP1/ABI1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9416-abi1-primary-antibodies-ihc-testing-2.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IHC analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody (PB9416). <br> SSH3BP1/ABI1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSH3BP1/ABI1 Antibody (PB9416) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9416-abi1-primary-antibodies-ihc-testing-3.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IHC analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody (PB9416). <br> SSH3BP1/ABI1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SSH3BP1/ABI1 Antibody (PB9416) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9416-abi1-primary-antibodies-if-testing-4.jpgAnti-SSH3BP1/ABI1 Antibody Picoband&reg; IF analysis of SSH3BP1/ABI1 using anti-SSH3BP1/ABI1 antibody (PB9416). <br> SSH3BP1/ABI1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SSH3BP1/ABI1 Antibody (PB9416) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ache-picoband-trade-antibody-pb9417-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9417-1-WB-anti-ache-acetylcholinesterase-picoband-antibody.jpgAnti-Acetylcholinesterase/ACHE Antibody Picoband&reg; Western blot analysis of ACHE using anti-ACHE antibody (PB9417). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Kidney Tissue Lysate at 50ug,<br> Lane 2: Mouse Liver Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: PANC Whole Cell Lysate at 40ug,<br> Lane 5: COLO320 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACHE antigen affinity purified polyclonal antibody (Catalog # PB9417) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACHE at approximately 68 kDa. The expected band size for ACHE is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adipor1-picoband-trade-antibody-pb9418-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9418-1-WB-anti-adipor1-adiponectin-receptor-1-picoband-antibody.jpgAnti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband&reg; Western blot analysis of ADIPOR1 using anti-ADIPOR1 antibody (PB9418). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Thymus Tissue Lysate at 50ug,<br> Lane 2: Rat Testis Tissue Lysate at 50ug,<br> Lane 3: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 4: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADIPOR1 antigen affinity purified polyclonal antibody (Catalog # PB9418) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADIPOR1 at approximately 43 kDa. The expected band size for ADIPOR1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9418-2.jpgAnti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband&reg; IF analysis of ADIPOR1 using anti-ADIPOR1 antibody (PB9418). <br> ADIPOR1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ADIPOR1 Antibody (PB9418) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ahr-picoband-trade-antibody-pb9419-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9419-1_1-WB-anti-ahr-picoband-antibody.jpgAnti-Aryl hydrocarbon Receptor/AHR Antibody Picoband&reg; Western blot analysis of AHR using anti-AHR antibody (PB9419). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Liver Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody (Catalog # PB9419) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHR at approximately 96 kDa. The expected band size for AHR is at 96 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-annexin-a3-picoband-trade-antibody-pb9420-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9420-anxa3-primary-antibodies-wb-testing-1.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; Western blot analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human CACO-2 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human SH-SY5Y whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A3 antigen affinity purified polyclonal antibody (Catalog # PB9420) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A3 at approximately 36 kDa. The expected band size for Annexin A3 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9420-2-IHC-anti-annexin-a3-picoband-antibody.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420). <br> Annexin A3 was detected in paraffin-embedded section of Mouse Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Annexin A3 Antibody (PB9420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9420-3-IHC-anti-annexin-a3-picoband-antibody.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420). <br> Annexin A3 was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Annexin A3 Antibody (PB9420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9420-4-IHC-anti-annexin-a3-picoband-antibody.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420). <br> Annexin A3 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Annexin A3 Antibody (PB9420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9420-5-IHC-anti-annexin-a3-picoband-antibody.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420).<br> Annexin A3 was detected in frozen section of IHC(F): Mouse Cardiac Muscle Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Annexin A3 Antibody (PB9420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9420-6-IHC-anti-annexin-a3-picoband-antibody.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420).<br> Annexin A3 was detected in frozen section of IHC(F): Rat Cardiac Muscle Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Annexin A3 Antibody (PB9420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9420-7-IHC-anti-annexin-a3-picoband-antibody.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420).<br> Annexin A3 was detected in frozen section of IHC(F): Human Placenta Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Annexin A3 Antibody (PB9420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9420-8.jpgAnti-Annexin A3/ANXA3 Antibody Picoband&reg; IHC analysis of Annexin A3 using anti-Annexin A3 antibody (PB9420).<br> Annexin A3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Annexin A3 Antibody (PB9420) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aph1a-picoband-trade-antibody-pb9421-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9421-aph1a-primary-antibodies-wb-testing-1.jpgAnti-APH1a Antibody Picoband&reg; Western blot analysis of APH1A using anti-APH1A antibody (PB9421). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APH1A antigen affinity purified polyclonal antibody (Catalog # PB9421) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APH1A at approximately 29 kDa. The expected band size for APH1A is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ataxin-1-picoband-trade-antibody-pb9422-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9422-ataxin-1-primary-antibodies-wb-testing-1.jpgAnti-Ataxin 1/ATXN1 Antibody Picoband&reg; Western blot analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 1 antigen affinity purified polyclonal antibody (Catalog # PB9422) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 1 at approximately 105 kDa. The expected band size for Ataxin 1 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9422-ataxin-1-primary-antibodies-ihc-testing-2.jpgAnti-Ataxin 1/ATXN1 Antibody Picoband&reg; IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). <br> Ataxin 1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ataxin 1 Antibody (PB9422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9422-ataxin-1-primary-antibodies-fcm-testing-6.jpgAnti-Ataxin 1/ATXN1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-Ataxin 1 antibody (PB9422). <br>Overlay histogram showing SiHa cells stained with PB9422 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ataxin 1 Antibody (PB9422, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9422-ataxin-1-primary-antibodies-ihc-testing-3.jpgAnti-Ataxin 1/ATXN1 Antibody Picoband&reg; IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). <br> Ataxin 1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ataxin 1 Antibody (PB9422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9422-ataxin-1-primary-antibodies-ihc-testing-4.jpgAnti-Ataxin 1/ATXN1 Antibody Picoband&reg; IHC analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). <br> Ataxin 1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ataxin 1 Antibody (PB9422) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9422-ataxin-1-primary-antibodies-if-testing-5.jpgAnti-Ataxin 1/ATXN1 Antibody Picoband&reg; IF analysis of Ataxin 1 using anti-Ataxin 1 antibody (PB9422). <br> Ataxin 1 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- Ataxin 1 Antibody (PB9422) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ataxin-3-picoband-trade-antibody-pb9423-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9423-1-WB-anti-ataxin-3-picoband-antibody.jpgAnti-Ataxin 3/ATXN3 Antibody Picoband&reg; Western blot analysis of Ataxin 3 using anti-Ataxin 3 antibody (PB9423). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate<br> Lane 2: COLO320 Whole Cell Lysate<br> Lane 3: HELA Whole Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9423) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 3 at approximately 42KD. The expected band size for Ataxin 3 is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9423-2-IHC-anti-ataxin-3-picoband-antibody.jpgAnti-Ataxin 3/ATXN3 Antibody Picoband&reg; IHC analysis of Ataxin 3 using anti-Ataxin 3 antibody (PB9423). <br> Ataxin 3 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ataxin 3 Antibody (PB9423) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9423-atxn3-primary-antibodies-fc-testing-3.jpgAnti-Ataxin 3/ATXN3 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-ATXN3 antibody (PB9423). <br>Overlay histogram showing A549 cells stained with PB9423 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATXN3 Antibody (PB9423&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9423-atxn3-primary-antibodies-if-testing-4.jpgAnti-Ataxin 3/ATXN3 Antibody Picoband&reg; IF analysis of ATXN3 using anti-ATXN3 antibody (PB9423).<br> ATXN3 was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ATXN3 Antibody (PB9423) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-a2-picoband-trade-antibody-pb9424-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-fimmu-15-1426474-g010.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg;CCNA2 is highly expressed in PRAD and is associated with poor patient prognosis. (A, B) Differential expression of CCNA2 in PRAD. (C) Diagnostic predictive value of CCNA2 in PRAD. (D) KM curve of overall survival of CCNA2 in PRAD. (E) Prognostic predictive value of CCNA2 in PRAD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1426474/full'>38947325</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-fimmu-15-1426474-g009.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg;CCNA2 has a high binding capacity to PRAD-targeted drugs. (A) Analysis of the binding capacity of CCNA2 to PD1 inhibitors. (B) Analysis of the binding capacity of CCNA2 to bicalutamide. (C) Analysis of the binding capacity of CCNA2 to enzalutamide. (D) Analysis of the binding capacity of CCNA2 to abiraterone.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1426474/full'>38947325</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-fimmu-15-1426474-g007.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg;CCNA2 positively correlates with monocyte infiltration levels. (A) Correlation analysis of CCNA2 and monocyte infiltration levels. (B, C) Analysis of CCNA2 correlation with monocyte markers. (D) Mendelian randomization analysis of high HLA-DR expressing monocytes in relation to prostate cancer. (E–I) Single-cell analysis of the correlation between CCNA2 and immune cell infiltration.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1426474/full'>38947325</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-fimmu-15-1426474-g006.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg;CCNA2 identified as the best prognostic gene among monocyte-associated genes. (A, B) Functional analysis of monocyte-related prognostic genes. (C, D) GBM and RandomForest algorithms to screen key prognostic genes in the TCGA-PRAD dataset. (E, F) GBM and RandomForest algorithms to screen key prognostic genes in the GSE16560 dataset. (G, H) KM curves of CCNA2 and ACSM3 in the TCGA-PRAD dataset. (I, J) KM curves of CCNA2 and ACSM3 in the GSE16560 dataset.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1426474/full'>38947325</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-fimmu-15-1426474-g008.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg;Functional analysis of CCNA2 in PRAD. (A) KEGG analysis of CCNA2 in PRAD. (B–J) GSEA analysis of CCNA2 in PRAD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2024.1426474/full'>38947325</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-ccna2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg; Western blot analysis of Cyclin A2 using anti-Cyclin A2 antibody (PB9424). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human HL-60 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: rat stomach tissue lysates, <br> Lane 5: rat testis tissue lysates, <br> Lane 6: mouse stomach tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A2 antigen affinity purified polyclonal antibody (Catalog # PB9424) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A2 at approximately 55 kDa. The expected band size for Cyclin A2 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-ccna2-primary-antibodies-fcm-testing-4.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Cyclin A2 antibody (PB9424). <br>Overlay histogram showing U20S cells stained with PB9424 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin A2 Antibody (PB9424, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-ccna2-primary-antibodies-ihc-testing-2.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg; IHC analysis of Cyclin A2 using anti-Cyclin A2 antibody (PB9424). <br> Cyclin A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cyclin A2 Antibody (PB9424) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-ccna2-primary-antibodies-if-testing-3.jpgAnti-Cyclin A2/CCNA2 Antibody Picoband&reg; IF analysis of Cyclin A2 using anti-Cyclin A2 antibody (PB9424). <br> Cyclin A2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cyclin A2 Antibody (PB9424) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9424-ccna2-primary-antibodies-wb-testing-2.pngAnti-Cyclin A2/CCNA2 Antibody Picoband&reg; Western blot analysis of Cyclin A2 using anti-Cyclin A2 antibody (PB9424). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Normal group-rat colon tissue lysates, <br> Lane 2: Model group-rat colon tissue lysates, <br> Lane 3: Triditional Chinese medicine treatment (low dose)-rat colon tissue lysates, <br> Lane 4: Triditional Chinese medicine treatment (medium dose)-rat colon tissue lysates, <br> Lane 5: Triditional Chinese medicine treatment(high dose)-rat colon tissue lysates, <br> Lane 6: Western medicine-rat colon tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A2 antigen affinity purified polyclonal antibody (Catalog # PB9424) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Cyclin A2 at approximately 55 kDa. The expected band size for Cyclin A2 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctbp1-picoband-trade-antibody-pb9425-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9425-ctbp1-primary-antibodies-wb-testing-1.jpgAnti-CtBP1 Antibody Picoband&reg; Western blot analysis of CTBP1 using anti-CTBP1 antibody (PB9425). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human MOLT-4 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse SP2/0 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP1 antigen affinity purified polyclonal antibody (Catalog # PB9425) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTBP1 at approximately 48 kDa. The expected band size for CTBP1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9425-2-IHC-anti-ctbp1-picoband-antibody.jpgAnti-CtBP1 Antibody Picoband&reg; IHC analysis of CTBP1 using anti-CTBP1 antibody (PB9425). <br> CTBP1 was detected in paraffin-embedded section of Mouse Thymus Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9425-3-IHC-anti-ctbp1-picoband-antibody.jpgAnti-CtBP1 Antibody Picoband&reg; IHC analysis of CTBP1 using anti-CTBP1 antibody (PB9425). <br> CTBP1 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9425-4-IHC-anti-ctbp1-picoband-antibody.jpgAnti-CtBP1 Antibody Picoband&reg; IHC analysis of CTBP1 using anti-CTBP1 antibody (PB9425). <br> CTBP1 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9425-5.jpgAnti-CtBP1 Antibody Picoband&reg; IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425) <br> CTBP1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9425-6.jpgAnti-CtBP1 Antibody Picoband&reg; IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425) <br> CTBP1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9425-7.jpgAnti-CtBP1 Antibody Picoband&reg; IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425).<br> CTBP1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CTBP1 Antibody (PB9425) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9425-ctbp1-primary-antibodies-if-testing-8.jpgAnti-CtBP1 Antibody Picoband&reg; IF analysis of CTBP1 using anti-CTBP1 antibody (PB9425) and anti-Tubulin Alpha antibody (M03989-3).<br> CTBP1 was detected in immunocytochemical section of T47D cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-CTBP1 Antibody (PB9425) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9425-9.pngAnti-CtBP1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-CTBP1 antibody (PB9425). <br> Overlay histogram showing U20S cells stained with PB9425 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP1 Antibody (PB9425&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctbp2-picoband-trade-antibody-pb9426-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-ctbp2-primary-antibodies-wb-testing-1.jpgAnti-CTBP2 Antibody Picoband&reg; Western blot analysis of CTBP2 using anti-CTBP2 antibody (PB9426). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human THP-1 whole cell lysates, <br> Lane 4: human HEK293 whole cell lysates, <br> Lane 5: human CACO-2 whole cell lysates, <br> Lane 6: human HELA whole cell lysates, <br> Lane 7: rat C6 whole cell lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTBP2 antigen affinity purified polyclonal antibody (Catalog # PB9426) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTBP2 at approximately 50KD. The expected band size for CTBP2 is at 50KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9426-2-IHC-anti-ctbp2-picoband-antibody.jpgAnti-CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PB9426). <br> CTBP2 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9426-3-IHC-anti-ctbp2-picoband-antibody.jpgAnti-CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PB9426). <br> CTBP2 was detected in paraffin-embedded section of Rat Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9426-4-IHC-anti-ctbp2-picoband-antibody.jpgAnti-CTBP2 Antibody Picoband&reg; IHC analysis of CTBP2 using anti-CTBP2 antibody (PB9426). <br> CTBP2 was detected in paraffin-embedded section of Mouse Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-5.jpgAnti-CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 using anti-CTBP2 antibody (PB9426) <br> CTBP2 was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-6.jpgAnti-CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 using anti-CTBP2 antibody (PB9426) <br> CTBP2 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C.Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-7.jpgAnti-CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 using anti-CTBP2 antibody (PB9426).<br> CTBP2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-8.pngAnti-CTBP2 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-CTBP2 antibody (PB9426). <br> Overlay histogram showing SiHa cells stained with PB9426 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP2 Antibody (PB9426&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-9.pngAnti-CTBP2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-CTBP2 antibody (PB9426). <br> Overlay histogram showing A431 cells stained with PB9426 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP2 Antibody (PB9426&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-10.jpgAnti-CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 using anti-CTBP2 antibody (PB9426).<br> CTBP2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-11.jpgAnti-CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 using anti-CTBP2 antibody (PB9426) <br> P-cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-12.jpgAnti-CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 using anti-CTBP2 antibody (PB9426) <br> P-cadherin was detected in paraffin-embedded section of human colon cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9426-13.jpgAnti-CTBP2 Antibody Picoband&reg; IF analysis of CTBP2 using anti-CTBP2 antibody (PB9426) <br> P-cadherin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-CTBP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-heparanase-1-picoband-trade-antibody-pb9427-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9427-1-WB-anti-heparanase-1-picoband-antibody.jpgAnti-Heparanase 1/HPSE Antibody Picoband&reg; Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody (PB9427). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Human Placenta Tissue Lysate at 50ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody (Catalog # PB9427) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 61 kDa. The expected band size for Heparanase 1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-41598_2021_91777_fig1_html.pngAnti-Heparanase 1/HPSE Antibody Picoband&reg;( a ) Representative images of rat lungs after procurement. ( b ) Heparanase activity was detected in tissue after procurement following 1 h of warm ischemia. ( c ) Ultrastructural images of the endothelial glycocalyx (eGC) from lung grafts after 1 h of warm ischemia obtained using transmission electron microscopy. The labeled glycocalyx appears as the darker cell surface layer; black arrow indicates glycocalyx desquamated from the surface of an endothelial cell. *p < 0.05, **p < 0.01. NL native lungs, Ctrl control lungs with eGC damage induced by 1 h of ischemia, Hep lungs with eGC preserved by heparin administration prior to 1 h of ischemia, NAH lungs with intact eGC preserved by N-acetyl heparin administration prior to 1 h of ischemia. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-91777-0'>34112915</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-41598_2021_91777_fig2_html.pngAnti-Heparanase 1/HPSE Antibody Picoband&reg;The effect of eGC damage in the lung grafts on the posttransplant graft function and quality. Grafts were evaluated 2 h after transplantation for ( a ) PaO2/FiO2 (P/F ratio), ( b ) macroscopic morphology (representative samples are shown), ( c ) Wet-to-dry (W/D) ratio, and ( d ) microscopic morphology (representative H&E stained samples are shown). *p < 0.05, **p < 0.01. NL native lungs, Ctrl control lungs with eGC damage prior to transplant, Hep lungs with eGC preserved by heparin prior to transplant, NAH lungs with eGC preserved by N-acetyl heparin prior to transplant, I/R injury ischemia reperfusion injury. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-91777-0'>34112915</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-41598_2021_91777_fig3_html.pngAnti-Heparanase 1/HPSE Antibody Picoband&reg;Endothelial glycocalyx influences the inflammation and inflammatory cell migration in lung grafts after transplantation. ( a ) Real-time RT-PCR for the mRNA of proinflammatory cytokines interleukin (IL)-6 and IL-1β . ( b ) The extravasation of neutrophils, T cells, and monocytes in the transplanted grafts were evaluated by specific staining of polymorphonuclear neutrophils (naphthol staining, positive cells indicated by black arrow heads), T cells (CD3 + -positive, indicated by white arrows) and macrophages (CD68 + -positive, purple staining), and ( c ) quantitated. *p < 0.05, **p < 0.01. NL native lungs, Ctrl control lungs with eGC damage prior to transplant, Hep lungs with eGC preserved by heparin prior to transplant, NAH lungs with eGC preserved by N-acetyl heparin prior to transplant. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-91777-0'>34112915</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-41598_2021_91777_fig4_html.pngAnti-Heparanase 1/HPSE Antibody Picoband&reg;Assessment of endothelial glycocalyx and microvascular integrity in lung grafts after reperfusion. ( a ) Western blot of syendecan-1 in fractionated lung tissue 2 h after transplantation. Gel image depicts a single representative sample from each treatment group. Vascular endothelial cadherin (VE-Cad) and heat shock protein 90 (HSP90) were blotted as loading markers for the plasma membrane and cytosolic protein fractions, respectively. A full-length image of this blot and the blots showing multiple samples for each treatment group are shown in Supplemental Figs. – . Cyt cytosol protein fraction, M membrane protein fraction. ( b ) Glycosaminoglycan (GAG) content in the grafts 2 h after transplantation. ( c ) Syndecan-1 staining (yellow) in the peripheral vasculature (φ ~ 50 mm) of the grafts 2 h after transplantation. The endothelial luminal surface is indicated as a white line in the merged images (right column). Nuclei were stained using Hoechst 33342. BAF background autofluorescence, RBCs red blood cells, VL vascular lumen. ( d ) Pulmonary vascular resistance (PVR) of lung grafts during ex vivo lung perfusion (EVLP). Time-dependent fold changes from the values at 1 h are shown. n = 4–5 for each group. ( e ) Endothelial barrier integrity after EVLP was assessed by measuring the amount of Evans blue dye (EBD) retained in the tissue. ( f ) Time-dependent changes of syndecan-1 in the perfusate during EVLP. *p < 0.05, **p < 0.01. NL native lungs, Ctrl control lungs with eGC damage prior to transplant/EVLP, Hep lungs with eGC preserved by heparin treatment prior to transplant or EVLP, NAH lungs with eGC preserved by N-acetyl heparin treatment prior to transplant or EVLP. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-91777-0'>34112915</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-41598_2021_91777_fig5_html.pngAnti-Heparanase 1/HPSE Antibody Picoband&reg;The effects of heparanase inhibition on MMP-2 and MMP-9 activity. ( a ) The mRNA expression of metalloprotease (MMP)-9 in lung grafts 2 h after transplantation. ( b ) Representative gelatin zymography of time-dependent changes in activity of secreted MMP-2 and MMP-9 in EVLP perfusate. Full length of gels and replications are shown in Supplemental Fig. . ( c ) MMP-2 activity quantitated and shown as fold change. *p < 0.05, **p < 0.01. NL native lungs, Ctrl control, Hep heparin, NAH N-acetyl heparin. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-91777-0'>34112915</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-12933_2013_article_642_fig1_html.jpgAnti-Heparanase 1/HPSE Antibody Picoband&reg;Viability analysis of Ana-1 macrophages after treatment with AGEs, LY294002, anti-RAGE or HPA antibody. Cell viability assay is performed using MTT assay. A , Cells (5 × 10 4 ) were treated with AGEs (0, 25, 50, 100, 200 and 400 mg/L) for 3, 6, 12, 24 h. B , Cells (5 × 10 4 ) were pretreated with LY294002 (7.5-120 μM) for 1 h before culture with 100 mg/L AGEs for 3, 6, 12, 24 h. C , Cells (5 × 10 4 ) were pretreated with anti-RAGE or HPA antibody for 1 h before culture with 100 mg/L AGEs for 3, 6, 12, 24 h. The results represent the mean of six culture wells (mean ± SEM). *p < 0.05, as compared to the control group. All of the experiments were performed independently in triplicate. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1475-2840-12-37'>23442498</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-12933_2013_article_642_fig2_html.jpgAnti-Heparanase 1/HPSE Antibody Picoband&reg;HPA, RAGE and PI3K/AKT pathway correlate with AGEs-induced macrophage migration. Cells were cultured with AGEs for 24 h with or without pre-treatment with LY294002, anti-HPA or RAGE antibody for 1 h. The migration was measured by transwell assays. Results were normalized to the number of macrophages that migrated in control group. The results represent the mean of six culture wells (mean ± SEM). *p < 0.05 compared to control and #p <0.05 compared to AGEs. All of the experiments were performed independently in triplicate. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1475-2840-12-37'>23442498</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-12933_2013_article_642_fig3_html.jpgAnti-Heparanase 1/HPSE Antibody Picoband&reg;AGEs up-regulates HPA mRNA, protein expression and secretion in macrophages via RAGE. Cells were cultured with AGEs for 24 h with or without pre-treatment with antibody against RAGE for 1 h. A , The levels of HPA mRNA were assessed with real time quantitative RT-PCR. B , The secretion of HPA in supernatant was measured by enzyme-linked immunosorbent assay (ELISA). C , The expression of HPA protein in macrophages was determined by Western blotting. The results represent the mean of six culture wells (mean ± SEM). *p < 0.05 compared to control and #p <0.05 compared to AGEs. All of the experiments were performed independently in triplicate. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1475-2840-12-37'>23442498</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9427-12933_2013_article_642_fig4_html.jpgAnti-Heparanase 1/HPSE Antibody Picoband&reg;The expression of AKT protein in AGEs-induced macrophages. Cells were cultured with AGEs or pretreated with antibody against RAGE or HPA for 1 h before exposed to AGEs for 24 h. AKT and p-AKT protein expression is determined by Western blot analysis using anti-AKT and p-AKT antibody. The results represent the mean of six culture wells (mean ± SEM). * P < 0.05 compared to control and # P <0.05 compared to AGEs. All of the experiments were performed independently in triplicate. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1475-2840-12-37'>23442498</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-itpr3-picoband-trade-antibody-pb9428-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9428-itpr3-primary-antibodies-wb-testing-1.jpgAnti-ITPR3 Antibody Picoband&reg; Western blot analysis of ITPR3 using anti-ITPR3 antibody (PB9428). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITPR3 antigen affinity purified polyclonal antibody (Catalog # PB9428) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITPR3 at approximately 270-300 kDa. The expected band size for ITPR3 is at 304 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9428-2-IHC-anti-itpr3-ip3r3-picoband-antibody.jpgAnti-ITPR3 Antibody Picoband&reg; IHC analysis of ITPR3 using anti-ITPR3 antibody (PB9428). <br> ITPR3 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITPR3 Antibody (PB9428) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9428-3-IHC-anti-itpr3-ip3r3-picoband-antibody.jpgAnti-ITPR3 Antibody Picoband&reg; IHC analysis of ITPR3 using anti-ITPR3 antibody (PB9428). <br> ITPR3 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITPR3 Antibody (PB9428) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9428-4-IHC-anti-itpr3-ip3r3-picoband-antibody.jpgAnti-ITPR3 Antibody Picoband&reg; IHC analysis of ITPR3 using anti-ITPR3 antibody (PB9428). <br> ITPR3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITPR3 Antibody (PB9428) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kdr-picoband-trade-antibody-pb9429-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9429-1-WB-anti-vegfr2-kdr-antibody.jpgAnti-VEGF Receptor 2/KDR Antibody Picoband&reg; Western blot analysis of KDR using anti-KDR antibody (PB9429). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: MCF-7 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDR antigen affinity purified polyclonal antibody (Catalog # PB9429) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDR at approximately 152 kDa. The expected band size for KDR is at 152 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-plectin-picoband-trade-antibody-pb9430-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9430-plectin-primary-antibodies-wb-testing-1.jpgAnti-Plectin Antibody Picoband&reg; Western blot analysis of Plectin using anti-Plectin antibody (PB9430). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plectin antigen affinity purified polyclonal antibody (Catalog # PB9430) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plectin at approximately 532 kDa. The expected band size for Plectin is at 532 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9430-plectin-primary-antibodies-ihc-testing-2.jpgAnti-Plectin Antibody Picoband&reg; IHC analysis of Plectin using anti-Plectin antibody (PB9430). <br> Plectin was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Plectin Antibody (PB9430) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9430-plectin-primary-antibodies-ihc-testing-3.jpgAnti-Plectin Antibody Picoband&reg; IHC analysis of Plectin using anti-Plectin antibody (PB9430). <br> Plectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Plectin Antibody (PB9430) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9430-plectin-primary-antibodies-ihc-testing-4.jpgAnti-Plectin Antibody Picoband&reg; IHC analysis of Plectin using anti-Plectin antibody (PB9430). <br> Plectin was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Plectin Antibody (PB9430) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9430-plectin-primary-antibodies-ihc-testing-5.jpgAnti-Plectin Antibody Picoband&reg; IHC analysis of Plectin using anti-Plectin antibody (PB9430). <br> Plectin was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Plectin Antibody (PB9430) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9430-plectin-primary-antibodies-if-testing-6.jpgAnti-Plectin Antibody Picoband&reg; IF analysis of Plectin using anti-Plectin antibody (PB9430). <br> Plectin was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Plectin Antibody (PB9430) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rac1-picoband-trade-antibody-pb9431-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9431-rac1-primary-antibodies-wb-testing-1.jpgAnti-Rac1 Antibody Picoband&reg; Western blot analysis of Rac1 using anti-Rac1 antibody (PB9431). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human Hacat whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat small intestine tissue lysates,<br> Lane 6: mouse small intestine tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rac1 antigen affinity purified polyclonal antibody (Catalog # PB9431) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rac1 at approximately 21 kDa. The expected band size for Rac1 is at 21 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9431-rac1-primary-antibodies-ihc-testing-2.jpgAnti-Rac1 Antibody Picoband&reg; IHC analysis of Rac1 using anti-Rac1 antibody (PB9431). <br> Rac1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Rac1 Antibody (PB9431) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9431-rac1-primary-antibodies-if-testing-3.jpgAnti-Rac1 Antibody Picoband&reg; IF analysis of Rac1 using anti-Rac1 antibody (PB9431) and anti-Beta Tubulin antibody (M01857-3).<br> Rac1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Rac1 Antibody (PB9431) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9431-rac1-primary-antibodies-fcm-testing-4.jpgAnti-Rac1 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-Rac1 antibody (PB9431). <br> Overlay histogram showing U87 cells stained with PB9431 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rac1 Antibody (PB9431, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-runx3-picoband-trade-antibody-pb9432-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9432-runx3-primary-antibodies-wb-testing-1.jpgAnti-RUNX3 Antibody Picoband&reg; Western blot analysis of RUNX3 using anti-RUNX3 antibody (PB9432). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates, <br> Lane 2: human Ramos whole cell lysates, <br> Lane 3: human HT1080 whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX3 antigen affinity purified polyclonal antibody (Catalog # PB9432) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX3 at approximately 43-48 kDa. The expected band size for RUNX3 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9432-2-IHC-anti-runx3-cbfa3-picoband-antibody.jpgAnti-RUNX3 Antibody Picoband&reg; IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9432). <br> RUNX3 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RUNX3 Antibody (PB9432) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9432-3-IHC-anti-runx3-cbfa3-picoband-antibody.jpgAnti-RUNX3 Antibody Picoband&reg; IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9432). <br> RUNX3 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RUNX3 Antibody (PB9432) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9432-4-IHC-anti-runx3-cbfa3-picoband-antibody.jpgAnti-RUNX3 Antibody Picoband&reg; IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9432). <br> RUNX3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RUNX3 Antibody (PB9432) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9432-5-IHC-anti-runx3-cbfa3-picoband-antibody.jpgAnti-RUNX3 Antibody Picoband&reg; IHC analysis of RUNX3 using anti-RUNX3 antibody (PB9432). <br> RUNX3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RUNX3 Antibody (PB9432) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9432-6.pngAnti-RUNX3 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-RUNX3 antibody (PB9432). <br> Overlay histogram showing THP-1 cells stained with PB9432 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUNX3 Antibody (PB9432&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9432-runx3-primary-antibodies-if-testing-7.jpgAnti-RUNX3 Antibody Picoband&reg; IF analysis of RUNX3 using anti-RUNX3 antibody (PB9432). <br> RUNX3 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RUNX3 Antibody (PB9432) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sdha-picoband-trade-antibody-pb9433-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9433-sdha-primary-antibodies-wb-testing-1.jpgAnti-SDHA Antibody Picoband&reg; Western blot analysis of SDHA using anti-SDHA antibody (PB9433). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SDHA antigen affinity purified polyclonal antibody (Catalog # PB9433) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SDHA at approximately 73 kDa. The expected band size for SDHA is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9433-sdha-primary-antibodies-ihc-testing-2.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PB9433). <br> SDHA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PB9433) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9433-sdha-primary-antibodies-ihc-testing-3.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PB9433). <br> SDHA was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PB9433) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9433-sdha-primary-antibodies-ihc-testing-4.jpgAnti-SDHA Antibody Picoband&reg; IHC analysis of SDHA using anti-SDHA antibody (PB9433). <br> SDHA was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SDHA Antibody (PB9433) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9433-sdha-primary-antibodies-if-testing-5.jpgAnti-SDHA Antibody Picoband&reg; IF analysis of SDHA using anti-SDHA antibody (PB9433). <br> SDHA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SDHA Antibody (PB9433) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9433-sdha-primary-antibodies-ip-testing-1.jpgAnti-SDHA Antibody Picoband&reg;Immunoprecipitating (IP) SDHA in HepG2 whole cell lysate.<br> Western blot analysis of SDHA using anti-SDHA antibody (PB9433); <br> Lane 1: HepG2 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-SDHA antibody in HepG2 whole cell lysate;<br> Lane 3: anti-SDHA antibody (2μg) + HepG2 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SDHA antigen affinity purified polyclonal antibody (PB9433) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SDHA at approximately 73 kDa. The expected band size for SDHA is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9433-sdha-primary-antibodies-fcm-testing-6.jpgAnti-SDHA Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-SDHA antibody (PB9433). <br> Overlay histogram showing 293T cells stained with PB9433 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (PB9433, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sgk1-picoband-trade-antibody-pb9434-boster.html2026-03-24T05:04:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9434-1-WB-anti-sgk1-sgk-picoband-antibody.jpgAnti-SGK1 Antibody Picoband&reg; Western blot analysis of SGK1 using anti-SGK1 antibody (PB9434). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HEPG2 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGK1 antigen affinity purified polyclonal antibody (Catalog # PB9434) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGK1 at approximately 49 kDa. The expected band size for SGK1 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc2a1-picoband-trade-antibody-pb9435-boster.html2026-03-25T05:22:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-1_1.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; Western blot analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysate&#44; <br> Lane 2: mouse brain tissue lysate&#44; <br> Lane 3: mouse NIH3T3 whole cell lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1 antigen affinity purified polyclonal antibody (Catalog # PB9435) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A1 at approximately 55KD. The expected band size for SLC2A1 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9435-2-IHC-anti-slc2a1-glut1-picoband-antibody.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9435-3-IHC-anti-slc2a1-glut1-picoband-antibody.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9435-4-IHC-anti-slc2a1-glut1-picoband-antibody.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9435-5-IHC-anti-slc2a1-glut1-picoband-antibody.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in frozen section of Human Placenta Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9435-6-IF-anti-slc2a1-glut1-picoband-antibody.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435).<br> SLC2A1 was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-wb-testing-7.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; Western blot analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: mouse brain tissue lysates, <br> Lane 4: rat PC-12 whole cell lysates, <br> Lane 5: mouse NIH/3T3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A1 antigen affinity purified polyclonal antibody (Catalog # PB9435) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A1 at approximately 55KD. The expected band size for SLC2A1 is at 55KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-ihc-testing-8.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-ihc-testing-9.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-ihc-testing-10.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-ihc-testing-11.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-ihc-testing-12.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of human placenta cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-ihc-testing-13.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IHC analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in paraffin-embedded section of human placenta cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-icc-testing-14.jpgAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; IF analysis of SLC2A1 using anti-SLC2A1 antibody (PB9435). <br> SLC2A1 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SLC2A1 Antibody (PB9435) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9435-slc2a1-primary-antibodies-fcm-testing-15.pngAnti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-SLC2A1 antibody (PB9435). <br>Overlay histogram showing A431 cells stained with PB9435 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC2A1 Antibody (PB9435, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc2a2-picoband-trade-antibody-pb9436-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9436-1-WB-anti-slc2a2-glut2-picoband-antibody.jpgAnti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband&reg; Western blot analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: PANC Whole Cell Lysate at 40ug,<br> Lane 2: A549 Whole Cell Lysate at 40ug,<br> Lane 3: HT1080 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A2 antigen affinity purified polyclonal antibody (Catalog # PB9436) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A2 at approximately 50 kDa. The expected band size for SLC2A2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9436-2-IHC-anti-slc2a2-glut2-picoband-antibody.jpgAnti-Glucose Transporter GLUT2/SLC2A2 Antibody Picoband&reg; IHC analysis of SLC2A2 using anti-SLC2A2 antibody (PB9436). <br> SLC2A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SLC2A2 Antibody (PB9436) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-band-3-picoband-trade-antibody-pb9437-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9437-slc4a1-primary-antibodies-wb-testing-1.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; Western blot analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human T-47D whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC4A1 antigen affinity purified polyclonal antibody (Catalog # PB9437) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC4A1 at approximately 102 kDa. The expected band size for SLC4A1 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9437-2_1-IHC-anti-band-3-picoband-antibody.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; IHC analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437). <br> SLC4A1 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC4A1 Antibody (PB9437) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9437-3_1-IHC-anti-band-3-picoband-antibody.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; IHC analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437). <br> SLC4A1 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC4A1 Antibody (PB9437) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9437-4-IHC-anti-band-3-picoband-antibody.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; IHC analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437). <br> SLC4A1 was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC4A1 Antibody (PB9437) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9437-5-IHC-anti-band-3-picoband-antibody.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; IHC analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437). <br> SLC4A1 was detected in frozen section of Human Placenta Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC4A1 Antibody (PB9437) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9437-6.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; IF analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437) <br> SLC4A1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-SLC4A1 Antibody (PB9437) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9437-slc4a1-primary-antibodies-ihc-testing-6.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; IHC analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437). <br> SLC4A1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SLC4A1 Antibody (PB9437) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9437-7.jpgAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; IF analysis of SLC4A1 using anti-SLC4A1 antibody (PB9437) <br> SLC4A1 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-SLC4A1 Antibody (PB9437) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9437-slc4a1-primary-antibodies-fcm-testing-8.pngAnti-SLC4A1/CD233/Band 3 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-SLC4A1 antibody (PB9437). <br> Overlay histogram showing K562 cells stained with PB9437, (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC4A1 Antibody (PB9437, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc6a4-picoband-trade-antibody-pb9438-boster.html2026-04-01T05:01:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-wb-testing-1.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg; Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates, <br> Lane 2: human U2OS whole cell lysates, <br> Lane 3: human SW620 whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat C6 whole cell lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse Neuro-2a whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (PB9438) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 70-90 kDa. The expected band size for SLC6A4 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-wb-testing-2.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg; Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human CACO-2 whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human SH-SY5Y whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (PB9438) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 70-90 kDa. The expected band size for SLC6A4 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-wb-testing-3.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg; Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates, <br> Lane 2: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (PB9438) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 70-90 kDa. The expected band size for SLC6A4 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-wb-testing-4_1.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg;Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438) .<br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 2: human 293T whole cell lysatess.<br> Lane 3: human MCF-7 whole cell lysatess.<br> Lane 4: human A549 whole cell lysatess.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (PB9438) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 10 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 95 kDa. The expected band size for SLC6A4 is at 95 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-wb-testing-5.pngAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg;Western blot analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438) .<br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Normal group-rat colon tissue lysates, <br> Lane 2: Model group-rat colon tissue lysates, <br> Lane 3: Triditional Chinese medicine treatment (low dose)-rat colon tissue lysates, <br> Lane 4: Triditional Chinese medicine treatment (medium dose)-rat colon tissue lysates, <br> Lane 5: Triditional Chinese medicine treatment(high dose)-rat colon tissue lysates, <br> Lane 6: Western medicine treatment-rat colon tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody (PB9438) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 10 minutes each and probed with a HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for SLC6A4 at approximately 95 kDa. The expected band size for SLC6A4 is at 95 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-ihc-testing-4.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg; IHC analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). <br> SLC6A4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-ihc-testing-5.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg; IHC analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). <br> SLC6A4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-if-testing-6.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg; IF analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). <br> SLC6A4 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9438-slc6a4-primary-antibodies-if-testing-7.jpgAnti-Serotonin transporter/SLC6A4 Antibody Picoband&reg; IF analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438). <br> SLC6A4 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/anti-slug-picoband-trade-antibody-pb9439-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9439-snai2-primary-antibodies-ihc-testing-3.jpgAnti-SLUG/SNAI2 Antibody Picoband&reg;IHC analysis of SNAI2 using anti-SNAI2 antibody (PB9439). <br>SNAI2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAI2 Antibody (PB9439) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9439-12885_2023_11796_fig6_html.pngAnti-SLUG/SNAI2 Antibody Picoband&reg;EMP3 affects the malignant phenotype of glioblastoma by promoting the EMT process ( A )-( D ). Correlation analysis of VIM , FOS , SNAI2 , TWIST1 and EMP3 using TCGA data ( E )-( H ). These related EMT markers were tested by qRT-PCR after the knockdown of EMP3 for 96 h cultured with siRNA. On the other hand, proteins of EMT markers VIM, SNAI2, FOS, TWIST1 were investigated by western blot after EMP3 siRNA transient transfection ( I )-( M ), Actin as the internal parameter. Cells were divided into three groups, NC (negtive control) group, siRNA EMP3-1 and siRNA EMP3-2 group. U87 cells were lysis after siRNA incubation for 72 h. The efficiency of trasfection of siRNA EMP3 was detected by WB ( N ). All of the full-length blots/gels of western blot are presented in Supplementary Figure C-H. * p < 0.05, ** P < 0.01, *** P < 0.001,**** p < 0.0001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://bmccancer.biomedcentral.com/articles/10.1186/s12885-023-11796-0'>38229014</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9439-snai2-primary-antibodies-wb-testing-1.jpgAnti-SLUG/SNAI2 Antibody Picoband&reg; Western blot analysis of SNAI2 using anti-SNAI2 antibody (PB9439). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: rat lung tissue lysates,<br> Lane 5: mouse lung tissue lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI2 antigen affinity purified polyclonal antibody (Catalog # PB9439) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI2 at approximately 30 kDa. The expected band size for SNAI2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9439-snai2-primary-antibodies-ihc-testing-2.jpgAnti-SLUG/SNAI2 Antibody Picoband&reg; IHC analysis of SNAI2 using anti-SNAI2 antibody (PB9439). <br> SNAI2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNAI2 Antibody (PB9439) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-snai3-picoband-trade-antibody-pb9440-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9440-1-WB-anti-snai3-picoband-antibody.jpgAnti-SNAI3 Antibody Picoband&reg; Western blot analysis of SNAI3 using anti-SNAI3 antibody (PB9440). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: MCF-7 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI3 antigen affinity purified polyclonal antibody (Catalog # PB9440) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI3 at approximately 32 kDa. The expected band size for SNAI3 is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-snrpn-picoband-trade-antibody-pb9441-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-wb-testing-1.jpgAnti-SNRPN Antibody Picoband&reg; Western blot analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human THP-1 whole cell lysates, <br> Lane 4: human RT4 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPN antigen affinity purified polyclonal antibody (Catalog # PB9441) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPN at approximately 25 kDa. The expected band size for SNRPN is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-ihc-testing-2.jpgAnti-SNRPN Antibody Picoband&reg; IHC analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-ihc-testing-3.jpgAnti-SNRPN Antibody Picoband&reg; IHC analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-ihc-testing-4.jpgAnti-SNRPN Antibody Picoband&reg; IHC analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-ihc-testing-5.jpgAnti-SNRPN Antibody Picoband&reg; IHC analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-ihc-testing-6.jpgAnti-SNRPN Antibody Picoband&reg; IHC analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-ihc-testing-7.jpgAnti-SNRPN Antibody Picoband&reg; IHC analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-if-testing-10.jpgAnti-SNRPN Antibody Picoband&reg; IF analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of human breast cnacer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-ihc-testing-8.jpgAnti-SNRPN Antibody Picoband&reg; IHC analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-if-testing-11.jpgAnti-SNRPN Antibody Picoband&reg; IF analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-if-testing-9.jpgAnti-SNRPN Antibody Picoband&reg; IF analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9441-snrpn-primary-antibodies-if-testing-12.jpgAnti-SNRPN Antibody Picoband&reg; IF analysis of SNRPN using anti-SNRPN antibody (PB9441). <br> SNRPN was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SNRPN Antibody (PB9441) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sod2-picoband-trade-antibody-pb9442-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9442-sod2-primary-antibodies-wb-testing-1.jpgAnti-SOD2 Antibody Picoband&reg; Western blot analysis of SOD2 using anti-SOD2 antibody (PB9442). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Human HepG2 whole cell lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: mouse liver tissue lysates,<br> Lane 5: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 antigen affinity purified polyclonal antibody (Catalog # PB9442) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 24 kDa. The expected band size for SOD2 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9442-dmso_a_12471889_f0008_c.jpgAnti-SOD2 Antibody Picoband&reg;DGLHT’s effect on liver protein expression Level in T2DM Mice.Three samples were analyzed for each group. The data were analyzed using an unpaired t-test. #P<0.05, ##P<0.01, ###P<0.001 vs Control group, *P<0.05, **P<0.01 vs Model group.Bar graphs show mean ± SD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/DMSO.S525913'>41146733</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9442-2-IHC-anti-sod2-mnsod-picoband-antibody.jpgAnti-SOD2 Antibody Picoband&reg; IHC analysis of SOD2 using anti-SOD2 antibody (PB9442).<br> SOD2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD2 Antibody (PB9442) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9442-sod2_-primary-antibodies-ihc-testing-3.jpgAnti-SOD2 Antibody Picoband&reg; IHC analysis of SOD2 using anti-SOD2 antibody (PB9442).<br> SOD2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SOD2 Antibody (PB9442) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9442-sod2_-primary-antibodies-ihc-testing-4.jpgAnti-SOD2 Antibody Picoband&reg; IHC analysis of SOD2 using anti-SOD2 antibody (PB9442).<br> SOD2 was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SOD2 Antibody (PB9442) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sp5-picoband-trade-antibody-pb9443-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9443-1-WB-anti-sp5-picoband-antibody.jpgAnti-Sp5 Antibody Picoband&reg; Western blot analysis of SP5 using anti-SP5 antibody (PB9443). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: MCF-7 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP5 antigen affinity purified polyclonal antibody (Catalog # PB9443) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP5 at approximately 42 kDa. The expected band size for SP5 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9443-2-IHC-anti-sp5-picoband-antibody.jpgAnti-Sp5 Antibody Picoband&reg; IHC analysis of SP5 using anti-SP5 antibody (PB9443). <br> SP5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP5 Antibody (PB9443) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sqstm1-p62-picoband-trade-antibody-pb9444-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-sqstm1-primary-antibodies-wb-testing-1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; Western blot analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: rat brain tissue lysates, <br> Lane 7: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 antigen affinity purified polyclonal antibody (Catalog # PB9444) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQSTM1 at approximately 62 kDa. The expected band size for SQSTM1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-fphar-16-1526653-g007.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;AEA improved CHF via PI3K/AKT/Bnip3 axis. (A) Representative images of PI3K/AKT axis. (B, C) The phosphorylation level of PI3K and AKT. (D) Representative images of Opa1, Drp1, Bnip3, p62, Atg5 and LC3II. (E–J) The expression level of Opa1, Drp1, Bnip3, p62, Atg5 and LC3II. (n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1526653/full'>40206063</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-fphar-16-1526653-g010.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;AEA improved NE-induced injuries via PI3K/AKT/Bnip3 axis. (A) Representative images of PI3K/AKT axis in H9c2 cells. (B, C) The phosphorylation level of PI3K and AKT. (D) Representative images of Opa1, Drp1, TrxR2, Bnip3, p62, Atg5 and LC3II in cells. (E–K) The expression level of Opa1, Drp1, TrxR2, Bnip3, p62, Atg5 and LC3II in H9c2 cells. (n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1526653/full'>40206063</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-fphar-16-1526653-g011.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;Immunofluorescence of Bnip3, Drp1, and p62 in H9c2 cells. (A) Immunofluorescence of Bnip3. (B) Immunofluorescence of Drp1. (C) Immunofluorescence of p62. (D) Quantitative analysis of Bnip3. (E) Quantitative analysis of Drp1. (F) Quantitative analysis of p62. (n = 3).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1526653/full'>40206063</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-nrr-15-2143-g006.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;ε-Viniferin drives the expression of mitochondrial homeostasis-related proteins. Western blot assays demonstrated that ε-viniferin treatment increases the expression levels of mitochondrial biosynthesis-related proteins (PGC-1α and TFAM); mitochondrial fusion and fission proteins (DRP1, MFN2, and FIS1), and mitophagy-related proteins (BNIP3, NIX, and Parkin), and decreases the expression levels of P62. Data are shown as the mean ± SD. # P < 0.05, vs . ε-viniferin treatment group (one-way analysis of variance followed by Bonferroni test). 1: Model group; 2: ε-viniferin treatment group; 3: ε-viniferin + SIRT3 shRNA group; 4: ε-viniferin + FOXO3 shRNA group; 5: ε-viniferin + control shRNA group. VFN: ε-Viniferin.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7716051/&orig_host=www.ncbi.nlm.nih.gov'>32394973</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-fphar-09-00301-g004.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;Effects of LLC on autophagy in the hearts from rats subjected to myocardial ischemia injury. (A) Representative transmission electron ultra-images showing autophagic vacuoles (marked with red arrows in the images, scale bar = 0.5 μm). (B) Quantitative analysis of the number of autophagic vacuoles in (A) . (C) Expression levels of LC3, Beclin 1 and p62 changes with LLC (80 mg⋅Kg -1 ) pretreatment. Data were presented as means ± SD. ∗∗ P < 0.01 vs. Con group, # P < 0.05, ## P < 0.01 vs. ISO group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00301/full'>29651246</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-fphar-09-00301-g005.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;Effects of LLC on autophagy in H9c2 cardiomyocytes under OGD. (A) Expression levels of LC3, Beclin 1 and p62 changes with LLC pretreatment. (B) Expression levels of LC3 and p62 changes in the presence or absence of 5 μmol⋅L -1 chloroquine and 40 μg⋅mL -1 LLC. (C) H9c2 cardiomyocytes were transfected with mRFP-GFP-LC3 and observed by fluorescent microscope (scale bar = 10 μm). (D) Mean number of autophagosomes represented by yellow dots in merged images and autolysosomes represented by red dots in merged images per cell. All experiments were repeated at least three times. Data were presented as means ± SD. ∗∗ P < 0.01 vs. Con group, ## P < 0.01 vs. OGD group, & P < 0.05, && P < 0.01 vs. OGD+LLC group, $ P < 0.05 vs. OGD+CQ group. CQ, chloroquine.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00301/full'>29651246</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-fphar-09-00301-g006.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;PI3K/Akt/mTOR pathway is involved in the cardioprotective effects of LLC. (A) Expression levels of PI3K, p-Akt, Akt, p-mTOR and mTOR in hearts were analyzed. (B) Representative western blots of PI3K (p110α), p-Akt (Ser473), Akt, p-mTOR (Ser2448), mTOR, LC3, Beclin 1 and p62 in the presence or absence of 20 μmol⋅L -1 LY294002 and 40 μg⋅mL -1 LLC. (C) The cell viability was analyzed by MTT assay. (D) The cell injury was detected by LDH measurements. All experiments were repeated at least three times. Data were presented as means ± SD. ∗∗ P < 0.01 vs. Con group, ## P < 0.01 vs. OGD group, & P < 0.05, && P < 0.01 vs. OGD+LLC group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00301/full'>29651246</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-ijbms-20-1029-g001.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;The changes in apoptosis and autophagy flux in spinal cord tissue after spinal cord injury. (A) The apoptosis in spinal cord tissue was detected by TUNEL staining (magnification 400x). (B) The protein levels of LC3I, LC3II and p62 in spinal cord tissue were assessed by Western blot assay. β-actin was used as a loading control. (C)&(D) The gray intensities of the bands were statistically analyzed and shown scale bar in A is 50 μm. The results shown represent at least three independent experiments. Each value represents the mean±SD (n=6) *** P <0.001, versus the sham group<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5651456/&orig_host=www.ncbi.nlm.nih.gov'>29085598</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-ijbms-20-1029-g003.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;The cell damage and impaired autophagy flux induced by mechanical injury in primary spinal cord neurons. (A) The primary spinal cord neurons were identified by immunofluoresence staining of NSE (magnification 400x). (B) The nucleus damage induced by MI in primary neurons of spinal cord was observed by Hoechst 33342 staining. (C-E) The protein expressions of LC3I, LC3II and p62 in primary spinal cord neurons were determined by Western blot analysis. β-actin was used as a loading control. The gray intensities of the bands were statistically analyzed and shown. Scale bar in A is 50 μm. The results shown represent at least three independent experiments. Each value represents the mean±SD (n=3). ** P <0.01, *** P <0.001, versus the control group MI on autophagy related proteins in primary neurons was assessed by Western blot. The results indicated that MI resulted in first increase and then decrease in LC3II/LC3I ratio (compared with control group, P <0.01) and p62 (compared with control group, P <0.001 and P <0.01, respectively) level in primary neurons over time, which was similar to that in spinal cord tissue after SCI.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5651456/&orig_host=www.ncbi.nlm.nih.gov'>29085598</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-ijbms-20-1029-g005.jpgAnti-SQSTM1/p62 Antibody Picoband&reg;Activation of AMP-activated protein kinase/sirtuin 1 pathway by resveratrol alleviated mechanical injury-induced cell damage and impaired autophagy flux in primary spinal cord neurons. (A-C) The protein levels of p-AMPK and SIRT1 in primary spinal cord neurons were assessed by Western blot assay and the statistical analysis of the gray intensities of the bands were shown. β-actin was used as a loading control. (D) The nucleus damage induced by MI in primary neurons of spinal cord was observed by Hoechst 33342 staining. (E-G) The protein levels of LC3I, LC3II and p62 in primary spinal cord neurons were assessed by Western blot assay. β-actin was used as a loading control. The gray intensities of the bands were statistically analyzed and shown. The results shown represent at least three independent experiments. Each value represents the mean±SD (n= 3). * P <0.05, ** P <0.01, *** P <0.001, versus the control group. # P <0.05, ## P <0.01, ### P <0.001, versus the MI group<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5651456/&orig_host=www.ncbi.nlm.nih.gov'>29085598</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-sqstm1-primary-antibodies-ihc-testing-2.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444).<br> SQSTM1 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-sqstm1-primary-antibodies-ihc-testing-3_1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444).<br> SQSTM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-4_1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). <br> SQSTM1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-sqstm1-primary-antibodies-ihc-testing-5.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444).<br> SQSTM1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-6_2.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IHC analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). <br> SQSTM1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-sqstm1-primary-antibodies-if-testing-7.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IF analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444).<br> SQSTM1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SQSTM1 Antibody (PB9444) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9444-8_1.jpgAnti-SQSTM1/p62 Antibody Picoband&reg; IF analysis of SQSTM1/p62 using anti-SQSTM1/p62 antibody (PB9444)<br> SQSTM1/p62 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-SQSTM1/p62 Antibody (PB9444) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tacr1-picoband-trade-antibody-pb9446-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9446-1-WB-anti-tacr1-nk1r-picoband-antibody.jpgAnti-Neurokinin 1 Receptor/TACR1 Antibody Picoband&reg; Western blot analysis of TACR1 using anti-TACR1 antibody (PB9446). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug,<br> Lane 2: Rat Lung Tissue Lysate at 50ug,<br> Lane 3: Rat Brain Tissue Lysate at 50ug,<br> Lane 4: U87 Whole Cell Lysate at 40ug,<br> Lane 5: A431 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TACR1 antigen affinity purified polyclonal antibody (Catalog # PB9446) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TACR1 at approximately 55 kDa. The expected band size for TACR1 is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mttfa-picoband-trade-antibody-pb9447-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-fimmu-14-1228374-g002.jpgAnti-mtTFA/TFAM Antibody Picoband&reg;Atenolol decreased the production of NETs induced by LPS. Fluorescence intensity for a representative animal of each group for the extracellular trap markers MPO (A) , H3 citr (C) , Sytox (E) and TFAM (G) in saline (blue), atenolol (orange), LPS (red) and LPS + atenolol (green). Quantification of mean fluorescence intensities (MFIs) in live neutrophils for the extracellular trap markers MPO (B) , H3 citr (D) , Sytox (F) and TFAM (H) for saline (blue), atenolol (orange), LPS (red) and LPS + atenolol (green) groups. Representative dot plot of living neutrophils co-expressing MPO and H3 citr in saline (I) , LPS (J) and LPS + atenolol (K) groups. Representative pictures of Ly6G+ neutrophils (green) labeling with citrullinated histone H3 (H3 citr) (red). The nucleus of cells was labeled with DAPI (in blue). Arrow shows a neutrophil producing an ET (L) . Representative pictures of Ly6G+ neutrophils (green) labeling with myeloperoxidase (MPO) (red). The nucleus of cells was labeled with DAPI (in blue) (M) . Saline n=16, atenolol n=6, LPS n=13, LPS + atenolol n=10. * p<0.05, ** p<0.01, *** p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10556451/&orig_host=www.ncbi.nlm.nih.gov'>37809074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-fimmu-14-1228374-g003.jpgAnti-mtTFA/TFAM Antibody Picoband&reg;Atenolol decreased the production of MiETs induced by LPS. Fluorescence intensity for a representative animal of each group for the extracellular trap markers MPO (A) , H3 citr (C) , Sytox (E) and TFAM (G) in saline (blue), atenolol (orange), LPS (red) and LPS + atenolol (green) groups. Quantification of mean fluorescence intensities (MFIs) in live microglia for the extracellular traps markers MPO (B) , H3 citr (D) , Sytox (F) and TFAM (H) for saline (blue), atenolol (orange), LPS (red) and LPS + atenolol (green) groups. Representative dot plot of living microglia co-expressing MPO and H3 citr in saline (I) , LPS (J) and LPS + atenolol (K) groups. Representative pictures of Iba1 + microglia (green) labeling with citrullinated histone H3 (H3 citr) (red). The nucleus of cells was labeled with DAPI (in blue) for saline (L) and LPS (M) groups. Saline n=16, atenolol n=6, LPS n=13, LPS + atenolol n=10. ** p<0.01, *** p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10556451/&orig_host=www.ncbi.nlm.nih.gov'>37809074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-fimmu-14-1228374-g005.jpgAnti-mtTFA/TFAM Antibody Picoband&reg;Sivelestat decreased the production of ETs induced by LPS. Quantification of mean fluorescence intensities (MFIs) of extracellular trap markers MPO (A) , H3 citr (B) , Sytox (C) and TFAM (D) for saline (blue), sivelestat (turquoise), LPS (red) and LPS + sivelestat (khaki) groups in live neutrophils. Quantification of MFI of extracellular traps markers MPO (E) , H3 citr (F) , Sytox (G) and TFAM (H) for saline (blue), sivelestat (turquoise), LPS (red) and LPS + sivelestat (khaki) groups in live microglia. Representative dot plot of live neutrophils co-expressing MPO and H3 citr in saline (I) , LPS (J) and LPS + sivelestat (K) . Representative dot plot of living microglia co-expressing MPO and H3 citr in saline (L) , LPS (M) and LPS + sivelestat (N) groups. Saline n=16, sivelestat n=6, LPS n=13, LPS + sivelestat n=8. * p<0.05, ** p<0.01, ***p<0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10556451/&orig_host=www.ncbi.nlm.nih.gov'>37809074</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-fcell-10-862675-g007.jpgAnti-mtTFA/TFAM Antibody Picoband&reg;CXCR2 promotes mitochondrial dysfunction and tubular senescence in vitro . (A–J) Representative micrographs of western blot and quantitative statistical data show renal expression of (B) CXCR2 (C) active β-catenin, (D) PGC-1α, (E) COX1, (F) TFAM, (G) TOMM20, (H) p16 INK4A , (I) γ-H2AX and (J) FN in each group. * p < 0.05, ** p < 0.01, *** p < 0.001, versus the pcDNA3 group ( n = 3). (K,L) Graphical representations show the relative mRNA level of (K) CXCR2 and (L) p16 INK4A in different groups. *** p < 0.001 versus the pcDNA3 group ( n = 3). (M) Representative micrographs show SA-β-gal staining and γ-H2AX expression in different groups. Frozen sections were performed by SA-β-gal and γ-H2AX immunofluorescence staining. Arrows indicates positive staining. Scale bar = 50 or 10 μm. (N–R) Representative micrographs of western blot and quantitative statistical data show protein levels of (O) CXCR2, (P) TOMM20, (Q) p16 INK4A and (R) FN in each group. * p < 0.05 versus the control group ( n = 3). (S) Representative micrographs show SA-β-gal staining in each group. Frozen renal sections were performed by SA-β-gal staining. Arrows indicate positive staining. Scale bar, 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9110966/&orig_host=www.ncbi.nlm.nih.gov'>35592244</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-fphar-13-836496-g009.jpgAnti-mtTFA/TFAM Antibody Picoband&reg;O304 could promote autophagy and alleviate mitochondrial dysfunction in vitro . (A–D) Representative western blot showing the expression of p-mTOR, P62, and LC3B in different groups. HKC-8 cells were stimulated by D-Gal (10 mg/ml) for 60 h with or without O304 (50 nM). * p < 0.05 versus control (Ctrl) cells; # p < 0.05 versus D-Gal-treated group alone ( n = 3). (E) Representative micrographs show that O304 promoted acidic-pH LC3B-positive autophagolysosomes (red fluorescence). HKC-8 cells were pre-transfected with lentiviruses expressing RFP-GFP-LC3B for 12 h, and were then stimulated by D-Gal (10 mg/ml) for 60 h with or without O304 (50 nM). Natural-pH LC3B-positive autophagosomes (green fluorescence) and acidic-pH LC3B-positive autophagolysosomes (red fluorescence) were detected. (F–H) Representative western blot and quantitative data showing the expression of PGC-1α and TFAM. * p < 0.05 versus control group; # p < 0.05 versus D-Gal-treated group alone ( n = 3). (I) Representative micrographs showing the expression of mTOR, P62, TOMM20 and mitochondrial ROS production in different groups. HKC-8 cells were seeded on coverslips and stimulated by D-Gal (10 mg/ml) for 60 h with or without O304 (50 nM). The cells were immunostained with mitoSOX probe or antibodies against mTOR, P62 and TOMM20, respectively. Representative transmission electron microscopy (TEM) micrographs (middle) showing O304 protects the normal structure of mitochondria in HKC-8 cells. For mTOR, P62, TOMM20 and mitoSOX staining, arrows indicate positive staining. For TEM analyses, arrowheads indicate abnormal-shaped mitochondria.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8924548/&orig_host=www.ncbi.nlm.nih.gov'>35308246</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-mttfa-primary-antibodies-wb-testing-1.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; Western blot analysis of mtTFA using anti-mtTFA antibody (PB9447). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human Caco-2 whole cell lysates,<br> Lane 4: human Raji whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse brian tissue lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody (PB9447) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for mtTFA at approximately 24 kDa. The expected band size for mtTFA is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-mttfa-primary-antibodies-ihc-testing-2.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PB9447). <br> mtTFA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PB9447) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-mttfa-primary-antibodies-ihc-testing-3.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IHC analysis of mtTFA using anti-mtTFA antibody (PB9447). <br> mtTFA was detected in a paraffin-embedded section of human pancrease cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-mtTFA Antibody (PB9447) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-mttfa-primary-antibodies-if-testing-4.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; IF analysis of mtTFA using anti-mtTFA antibody (PB9447) and anti-Tubulin Alpha antibody (M03989-3).<br> mtTFA was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-mtTFA Antibody (PB9447) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9447-mttfa-primary-antibodies-ip-testing-5.jpgAnti-mtTFA/TFAM Antibody Picoband&reg; Immunoprecipitating (IP) mtTFA in K562 whole cell lysate.<br> Western blot analysis of mtTFA using anti-mtTFA antibody (PB9447); <br> Lane 1: K562 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-mtTFA antibody in K562 whole cell lysate;<br> Lane 3: anti-mtTFA antibody (2μg) + K562 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-mtTFA antigen affinity purified polyclonal antibody (PB9447) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for mtTFA at approximately 24 kDa. The expected band size for mtTFA is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tgm2-picoband-trade-antibody-pb9448-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-1-WB-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; Western blot analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Liver Tissue Lysate<br> Lane 2: Rat Ovary Tissue Lysate<br> Lane 3: HELA Whole Cell Lysate<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGM2 antigen affinity purified polyclonal antibody (Catalog # PB9448) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGM2 at approximately 77KD. The expected band size for TGM2 is at 80KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-2-IHC-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> TGM2 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGM2 Antibody (PB9448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-3-IHC-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> TGM2 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGM2 Antibody (PB9448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-4-IHC-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> TGM2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGM2 Antibody (PB9448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-5-IHC-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> TGM2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGM2 Antibody (PB9448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-6-IHC-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> TGM2 was detected in frozen section of mouse spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGM2 Antibody (PB9448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-7-IHC-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> TGM2 was detected in frozen section of rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGM2 Antibody (PB9448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9448-8-IHC-anti-tgm2-transglutaminase-2-picoband-antibody.jpgAnti-Transglutaminase 2/TGM2 Antibody Picoband&reg; IHC analysis of TGM2 using anti-TGM2 antibody (PB9448).<br> TGM2 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TGM2 Antibody (PB9448) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tyrosine-hydroxylase-picoband-trade-antibody-pb9449-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-th-primary-antibodies-wb-testing-1_1.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg; Western blot analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosine Hydroxylase/TH antigen affinity purified polyclonal antibody (Catalog # PB9449) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase/TH at approximately 59 kDa. The expected band size for Tyrosine Hydroxylase/TH is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-th-primary-antibodies-ihc-testing-1.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;IHC analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449). <br> Tyrosine Hydroxylase/TH was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydroxylase/TH Antibody (PB9449) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ajtr0011-2925-f5.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;The central administration of losartan or tempol prevented renal RAS activation. A. The central administration of losartan or tempol prevented renal RAS activation. Representative photographs and semiquantitative data of AGT, AT1 and MCP-1 expression by immunohistochemistry. B. The central administration of losartan or tempol did not prevente glomerularsclerosis, however only oral administration of drugs could alleviate glomerulosclerosis. Representative photographs and semiquantitative data of glomerulosclerosis index were shown by PAS. Data are expressed as the mean ± SD (n=6 in each group). * P <0.05 versus IG 0 mg/kg/d Los. PAS, periodic acid-Schiff.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-th-primary-antibodies-ihc-testing-2.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg; IHC analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449). <br> Tyrosine Hydroxylase/TH was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydroxylase/TH Antibody (PB9449) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g2.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Sleep apnea and hypnic occurrence rate in Cdkl5 KO mice. (A) Apnea occurrence rate during non-rapid-eye-movement sleep (NREM) sleep period in young adult Cdkl5 -/Y (n = 8) and Cdkl5 +/Y (n = 9) mice, and middle-aged Cdkl5 -/Y (n = 14) and Cdkl5 +/Y (n = 14) mice. (B) Percentage of time spent in wakefulness, in NREM, and in rapid-eye-movement sleep (REM) during whole-body-plethysmography recordings in the same animals as in A. Values represent mean ± SEM. *p< 0.05, **p< 0.01, ***p< 0.001 (Fisher’s LSD test after two-way ANOVA).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-th-primary-antibodies-ihc-testing-3.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg; IHC analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449). <br> Tyrosine Hydroxylase/TH was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tyrosine Hydroxylase/TH Antibody (PB9449) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ajtr0011-2925-f2.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Brain RAS, oxidative stress and sympathetic activity were up-regulated in DM rats. A. AGT and AT1 receptors in SFO (a1), SON (a2) and PVN (a3) measured by immunohistochemistry. B. AGT and AT1 receptors in SFO (b1), SON (b2) and PVN (b3) measured by Western blot. C. Protein levels of NOX2 and NOX4 in SFO (c1), SON (c2) and PVN (c3) measured by Western-blot. D. Representative photographs of TH+c-fos positive cells in RVLM measured by immunohistochemistry. E. Protein levels of TH in RVLM measured by Western-blot. F. Protein levels of TH in SFO, SON, PVN measured by Western-blot. Data are expressed as the mean ± SD (n=15 in each group). * P <0.05 versus Non-DM.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-th-primary-antibodies-if-testing-4_1.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg; IF analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449). <br> Tyrosine Hydroxylase/TH was detected in a paraffin-embedded section of mouse brian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Tyrosine Hydroxylase/TH Antibody (PB9449) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ajtr0011-2925-f4.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Central administration of tempol, but not clonidine, downregulate overexpression of brain RAS and sympathetic nerve activity in DM rats. A. Central administration of tempol downregulate overexpression of brain RAS in DM rats measured by immunohistochemistry. B. Central administration of losartan or tempol decreased sympathetic nerve activity in DM rats. Representative photographs of TH- and c-fos-positive neurons in RVLM measured by double immunohistochemical staining. Data are expressed as the mean ± SD (n=6 in each group). * P <0.05 versus IG 0 mg/kg/d Los.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-th-primary-antibodies-if-testing-5_1.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg; IF analysis of Tyrosine Hydroxylase/TH using antiTyrosine Hydroxylase/TH antibody (PB9449). <br> Tyrosine Hydroxylase/TH was detected in a paraffin-embedded section of rat brian tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Tyrosine Hydroxylase/TH Antibody (PB9449) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g7.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Increased neuronal cell death in Cdkl5 KO mice. (A-B) Quantification of cells positive for cleaved caspase-3 in the CA1 layer of the hippocampus (A) and in layer II/III of the somatosensory cortex (S1, B) from young adult Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 4) mice, and middle-aged Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 4) mice. (C) Representative images, one from each group, of cells, in the hippocampal CA1 region (upper panel) and in layer II/III of the somatosensory cortex (S1 , lower panel), immunopositive for cleaved caspase-3 (red) and stained with Hoechst (blue). Scale bar = 50 μm. (D) Quantification of the total number of TH-positive neurons in the substantia nigra (SN) and ventral tegmental area (VTA) from young adult Cdkl5 +/Y (n = 5) and Cdkl5 -/Y (n = 4) mice, and middle-aged Cdkl5 +/Y (n = 4) and Cdkl5 - /Y (n = 5) mice. (E) Representative images of tyrosine hydroxylase (TH) immunofluorescence staining in the VTA and SN of middle-aged Cdkl5 +/Y and Cdkl5 -/Y mice. Scale bar = 100 μm. The dotted boxes indicate the VTA region shown at a higher magnification in the right panel. Scale bar = 40 μm. Values are represented as means ± SE. *p<0.05, **p<0.01, ***p<0.001 (Fisher’s LSD test after two-way ANOVA).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g9.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Increased DNA damage in the hippocampus of Cdkl5 KO mice. (A-B) Representative fluorescent images of the CA3 (A) and CA1 (B) hippocampal regions of adult and middle-aged Cdkl5 +/Y and Cdkl5 -/Y mice, immunostained for γH2AX and counterstained with Hoechst. Scale bar = 500 μm (C) Quantification of γH2AX nuclear signal intensity in the CA3 and CA1 pyramidal layer of young adult Cdkl5 +/Y (n = 3) and Cdkl5 -/Y (n = 3) mice, and middle-aged Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 3) mice. (D) Western blot analysis of γH2AX levels normalized to GAPDH levels in the hippocampus of young adult Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 5) mice, and middle-aged Cdkl5 +/Y (n = 3) and Cdkl5 -/Y (n = 3) mice. (E) Western blot analysis of XRCC5 levels normalized to GAPDH levels in the hippocampus of young adult Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 5) mice, and middle-aged Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 3) mice. (F) Immunoblot images of γH2AX, XRCC5, and GAPDH levels in protein extracts from one animal of each experimental group. Data in Western blot analysis are expressed as a percentage of the values compared to young adult Cdkl5 +/Y mice. Values are represented as means ± SE. *p< 0.05 and **p< 0.01, ***p<0.001 (Fisher’s LSD test after two-way ANOVA).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fimmu-12-714244-g001.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Electroacupuncture prevented cisplatin-induced leukopenia. (A) Experimental flowchart depicting the time of the treatments of Cisplatin (C), electroacupuncture (E), and the analyses of body weight (W) and sample collection. (B) Representative peripheral blood flow cytometry analyses of neutrophils (LY6G + ), monocytes (LY6C + ), T (CD3 + ), and B (CD19 + ) lymphocytes and (C) Blood counts of specific subpopulation of leukocytes of mice with control (Veh), cisplatin alone (Cis; 3 mg/kg), or with electroacupuncture (EA) treatment (leukocytes, lymphocytes: n =6 per group; neutrophils, monocytes, T and B lymphocytes: Veh, n =6; Cis, n =6; EA, n =7). (D) Mice body weight curves treatment at day 0, 3, 7, 10, 14 ( n =6 per group), P values were calculated using two-way repeated-measures ANOVA. Data are mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001 vs Veh; # P < 0.05, ## P < 0.01 vs Cis.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.714244/full'>34552585</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g5.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Cdkl5 KO mice exhibit a reduction of the number of c-fos. (A, C, E) Representative examples of c-Fos staining obtained from the pyramidal cell layer of the hippocampal CA1 (A), and from the primary somatosensory S1 (C) and primary visual cortices V1 (E) of young adult and middle-aged Cdkl5 +/Y and Cdkl5 -/Y mice. Scale bar = 50μm. (B) Quantification of the density of c-Fos immunoreactive cells in the CA1 layer from young adult Cdkl5 +/Y (n = 6) and Cdkl5 -/Y (n = 7) mice, and middle-aged Cdkl5 +/Y (n = 3) and Cdkl5 -/Y (n = 4) mice. (D) Quantitative analysis of the density of c-Fos positive cells in the S1 cortex of young adult Cdkl5 +/Y (n = 8) and Cdkl5 -/Y (n = 10) mice, and middle-aged Cdkl5 +/Y (n = 4) and Cdkl5 - /Y (n = 3) mice. (F) Quantification of c-Fos positive cells in the V1 cortex of young adult Cdkl5 +/Y (n = 5) and Cdkl5 -/Y (n = 5) mice, and middle-aged Cdkl5 +/Y (n = 3) and Cdkl5 -/Y (n = 3) mice. Values are represented as means ± SE. *p<0.05, **p<0.01, ***p<0.001 (Fisher’s LSD test after two-way ANOVA).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fig-4-1x.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Localized Ang II in the PVN triggers fibrosis in the FA-CKD model via SNS activation. Outline of experimental design: PVN-specific deletion of Ang II type 1a receptor (AT1a) was achieved by injecting AAV-Cre into PVN of AT1a fl/fl mice. Blockade of sympathetic outflow was achieved by denervation of the post-obstructed kidney (kdn). (B) Kidney norepinephrine (NE) level in homogenates of the FA-CKD mice. (C) Kidney fibrosis was visualized by Masson trichrome staining in FA-CKD mice: representative images (left) and quantitative data (right). Scale bar, 50 um. (D) Level of collagen I (Co I) mRNA in kidney homogenates of the FA-CKD mice. Error bars, mean ± standard deviation ( n = 6 in each group). *, p < 0.01. One-way analysis of variance or t test with Bonferroni correction. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/18166/'>39346076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fig-5-1x.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Ang II signaling in PVN activates the PVN-RVLM pathway that enhances SNS discharge to the kidney. (A) Schematic showing the injection of a retrograde tracer, CTb-555, into the RVLM and retrograde labeling of PVN (→RVLM) neurons. The source for the component is from . (B) Expression of c-fos was detected in CTb-555 PVN neurons: representative images and summary of percentage of c-fos+ cell in CTb-555+ cells. Scale bar, 50 um. *, p < 0.01. (C) Schematic of the coronal brain section indicating the RVLM. (D) Immunostaining of c-fos and tyrosine hydroxylase (TH) in RVLM: representative images (left) and summary of percentage of c-fos+ cell in TH+ cells(right). Scale bar, 50 um. *, p < 0.01. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/18166/'>39346076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fig-3-1x.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Activated Ang II signaling in PVN enhances SNS discharge that promotes fibrosis in the FA-CKD mice. (A) Outline of experimental design: PVN-specific deletion of Ang II type 1a receptor (AT1a) was achieved by injecting AAV-Cre into PVN of AT1a fl/fl mice. (B) Immunostaining of Cre protein and in situ hybridization of AT1a mRNA in PVN. Scale bar, 50 um. (C) Level of AT1a mRNA in PVN homogenates. (D) Sympathetic nerve activity (SNA) in the kidney: representative raw records (left) and quantitative data (right). Scale bar, 1 s. (E) Kidney norepinephrine (NE) level in homogenates of the FA-CKD mice. (F) Kidney fibrosis was visualized by Masson trichrome staining in FA-CKD mice: representative images (left) and quantitative data (right). Scale bar, 50 um. (G) Level of collagen I (Co I) mRNA in kidney homogenates of the FA-CKD mice. Error bars, mean G standard deviation ( n = 6 in each group). *, p < 0.01. One-way analysis of variance or t test with Bonferroni correction. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/18166/'>39346076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fig-2-1x.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Folic acid-induced kidney injury in mice is accompanied by activity of the local renin-angiotensin system in the PVN. (A) Immunostaining for angiotensinogen (AGT) in paraventricular nucleus (PVN, Bregma −0.94 mm): representative images (left) and quantitative data (right). Scale bar, 100 um. (B) Level of AGT mRNA in PVN homogenates. (C) Immunostaining for angiotensin II (Ang II) in PVN (Bregma −0.94 mm): representative images (left) and quantitative data (right). Scale bar, 50 um (D) Concentration of Ang II in PVN homogenates. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/18166/'>39346076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fig-1-1x.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Kidney fibrosis in folic acid-induced kidney is accompanied by enhanced sympathetic nervous system (SNS) discharge to the kidney. (A) Kidney fibrosis was visualized by Masson trichrome staining in folic acid-induced kidney injury from mice at various time points: representative images (left) and quantitative data (right). Scale bar, 50 um. (B) Level of collagen I (Co I) mRNA in kidney homogenates of the folic acid-induced kidney injury mice. (C) Sympathetic nerve activity (SNA) in the folic acid-induced kidney: representative raw records (left) and quantitative data (right). Scale bar, 2s. (D) Levels of norepinephrine (NE) in kidney homogenates of the folic acid-induced kidney. *, p < 0.01 versus sham. Error bars, mean ± standard deviation ( n = 6 in each group). One-way analysis of variance or t test with Bonferroni correction. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/18166/'>39346076</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g3.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Impaired dendritic morphology in Cdkl5 KO mice. (A) Example of a Golgi-stained brain slice. The dotted boxes highlight the brain regions of the hippocampal formation (granular layer (DG) and hippocampal CA1 region (CA1)) and the visual cortex (V1) where dendritic length and spine density and maturation were evaluated. Scale bar = 500 μm. (B-C) Mean total dendritic length (B) and mean number of dendritic segments (C) of Golgi-stained granule cells of the dentate gyrus (DG) in adult Cdkl5 -/Y (n = 3) and Cdkl5 +/Y (n = 3) mice, and middle-aged Cdkl5 -/Y (n = 3) and Cdkl5 +/Y (n = 3) mice. (D) Examples of the reconstructed dendritic tree of Golgi-stained mature granule neurons of one animal from each experimental group. Scale bar = 100 μm. (E-F) Mean total dendritic length (E) and mean number of basal dendritic segments (F) of Golgi-stained pyramidal neurons of the primary visual cortex (V1, layer II/III) in adult Cdkl5 -/Y (n = 4) and Cdkl5 +/Y (n = 4) mice, and middle-aged Cdkl5 -/Y (n = 4) and Cdkl5 +/Y (n = 4) mice. (G-H) Dendritic spine density (number of spines per 10 μm) on apical dendrites of pyramidal neurons of the CA1 layer of the hippocampus (G) and pyramidal neurons of V1 (layer II/III; H) from adult Cdkl5 -/Y (n = 4 or 5, respectively) and Cdkl5 +/Y (n = 4) mice, and middle-aged Cdkl5 -/Y (n = 4) and Cdkl5 +/Y (n = 4) mice. Values are represented as means ± SE. *p< 0.05, **p< 0.01, ***p< 0.001 (Fisher’s LSD after two-way ANOVA).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fimmu-12-714244-g002.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Electroacupuncture preserved hematopoiesis in mice with cisplatin chemotherapy. (A) Representative H&E staining of tibia BM from mice with control (Veh), cisplatin alone (Cis; 3 mg/kg), or with electroacupuncture (EA) treatment (scale bar=20.0 μm) and (B) Histogram representation of BM hematopoietic cellularity of H&E staining analyzed by Image-Pro Plus 6.0 software ( n= 4 per group). (C) Representative H&E staining of tibia BM from mice with Veh, cisplatin alone or with EA treatment at high configuration (scale bar=10.0 μm). (D) Representative HistoFAXS Tissue Analysis of BM cell nuclei hematoxylin-shade-mean intensity, and quantitative analysis of BM cell density ( n =4 per group). (E) Flowchart of hematopoiesis and hematopoietic cells markers. (F) Representative flow cytometry analyses and (G) quantification of hematopoietic BM cell subpopulations (Positive cells events (%) = (the events in target gate/the total cell) × 100) ( n =6 per group). (H) Representative PI nuclear staining flow cytometry analyses in BM cell cycle (G 0 /G 1 , S, G 2 /M phases) and (I) Quantification of PI nuclear staining of BM cells in G 0 /G 1 , S, G 2 /M phases by ModFit 3.1 software ( n =6 per group). (J) Expression of cell cycle related genes in BM cells ( Ki67 : Veh, n =7; Cis, n =5; EA, n =7. Ccna2 : n =7 per group. Ccnd1 : Veh, n =5; Cis, n =4; EA, n =6. Ccne1 : Veh, n =6; Cis, n =4; EA, n =5). Data are mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001 vs Veh; # P < 0.05 vs Cis.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.714244/full'>34552585</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fimmu-12-714244-g003.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Analyses of expression and enrichment of electroacupuncture in the bone marrow of mice with cisplatin. (A) Scatter plots of differentially expressed genes (DEG)s in Cis vs Veh and EA vs Cis group Each probe is represented by a point with red and blue points showing up- and down-regulated genes defined above Log2 FC > 2. (B) Venn diagram and (C) PPI network analyses of DEGs results. (D) RT-qPCR analyses of factors related to extracellular matrix ( Col1a1 , Col1a2 ), ribosome ( Rpl14 , Rpl29 , Rpl32) , and PPAR signaling ( Fabp4 , Scd1) ( Col1a1 , Col1a2 : n =7 per group. Rpl14 , Rpl29 , Scd1 : n =6 per group. Rpl32 , Fabp4 : Veh, n =6; Cis, n =5; EA, n =6). Data are mean ± SEM, * P < 0.05, ** P < 0.01 vs Veh.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.714244/full'>34552585</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g6.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Reduced neuronal survival in Cdkl5 KO mice. (A) Quantification of Hoechst-positive cells in CA1 of hippocampal sections from young adult Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 4) mice, and middleaged Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 4) mice. (B) Quantification of pyknotic cells in the CA1 layer of the hippocampus from mice as in B. (C) Quantification of NeuN-positive cells in CA1 of hippocampal sections from young adult Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 4) mice, and middle-aged Cdkl5 +/Y (n = 5) and Cdkl5 -/Y (n = 4) mice. (D) Representative fluorescence images of cell layers in the hippocampal CA1 region from young adult and middle-aged Cdkl5 +/Y and Cdkl5 -/Y mice. Arrows indicate pyknotic nuclei. Scale bar = 100 μm. E) Representative fluorescence images of sections immunopositive for NeuN (red) and counterstained with Hoechst (blue) in the hippocampal CA1 region of one animal from each group. Scale bar = 100 μm. Values are represented as means ± SE. *p<0.05, **p<0.01, ***p<0.001 (Fisher’s LSD test after two-way ANOVA).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fimmu-12-714244-g004.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Neurogenic PACAP mediated electroacupuncture-induced protection to cisplatin. (A) Representative immunofluorescence images (Scale bar=20.0 μm) and (B) Quantification of sympathetic Th + fibers (red) and nuclear (blue) in the BM of the experimental mice ( n= 4 per group). (C) Expression analyses of neurotrophic factors ( Ngf , Bndf : Veh, n =7; Cis, n =6; EA, n =7. PACAP: Veh, n =5; Cis, n =6; EA, n =6). (D) Representation of the latency time (seconds) in hot-plate tests of mice treated with control (Veh), cisplatin (Cis; 3mg/kg), and cisplatin + electroacupuncture (EA) without or with PACAP6-38 (a blocker for PACAP receptor, PAC1) at low (10 μg/kg) or high (100 μg/kg) concentrations (Cis, n =7; other groups, n =8), P values were calculated using two-way repeated-measures ANOVA. (E) Peripheral blood counts of specific subpopulation of leukocytes (Veh, Cis, EA: n =6; other groups, n =7). (F) Analyses of hematopoietic BM subpopulation cells (Veh, Cis, EA, EA+PA6-38-L, EA+PA6-38-H: n =7; PA6-38-L, n =8, PA6-38-H, n =6). (G) Quantification of PI nuclear staining of BM cells (Veh, n =8; Cis, n =7; EA, n =8; EA+PA6-38-L, n =8; EA+PA6-38-H, n =7; PA6-38-L, n =8; PA6-38-H, n =7). Data are mean ± SEM * P < 0.05, ** P < 0.01, *** P < 0.001 vs Veh; # P < 0.05, ## P < 0.01, ### P < 0.001 vs Cis; ⋆ P < 0.05, ⋆⋆ P < 0.01 vs EA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.714244/full'>34552585</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g8.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Senescence-associated-β-galactosidase (SA-β-gal) activity in Cdkl5 KO mice. (A) Representative image at low magnification of a hippocampus of a middle-aged Cdkl5 -/Y mouse. Scale bar = 500 μm. The dotted boxes indicate the regions used for quantification in (D-F). (B-C) Higher magnification shows representative images of SA-β-gal staining in the hippocampal CA3 (B) and CA1 (C) region from adult and middle-aged Cdkl5 +/Y and Cdkl5 -/Y mice. Scale bar = 50 μm. (D-F) Quantification of SA-β-gal intensity in CA3 (D) and CA1 (E) hippocampal layers, and in layer II/III of the S1 cortex (F) from young adult Cdkl5 +/Y (n = 3) and Cdkl5 -/Y (n = 4) mice, and middle-aged Cdkl5 +/Y (n = 4) and Cdkl5 -/Y (n = 3) mice. Values are represented as means ± SE. *p< 0.05, **p< 0.01, ***p< 0.001 (Fisher’s LSD test after two-way ANOVA).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fimmu-12-714244-g005.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;PAC1-agonist mimics electroacupuncture-induced protection to cisplatin. (A) Representation of the latency time (seconds) in hot-plate tests of mice with control (Veh), cisplatin (Cis; 3 mg/kg), EA (cisplatin + electroacupuncture), cisplatin mice were treated with low (10 μg/kg) or high (50 μg/kg) concentrations PAC1-agonist, PACAP1-38 (Veh, n =8; Cis, n =7; EA, n =8; PA38-L, n =8; PA38-H, n =7), P values were calculated using two-way repeated-measures ANOVA. (B) Peripheral blood counts of specific subpopulation of leukocytes ((Veh, Cis, EA: n =6; other groups, n =7). (C) Analyses of hematopoietic BM cell subpopulation (Veh, Cis, EA: n =7; PA38-L, PA38-H: n =8). (D) Quantification of PI nuclear staining of BM cells (Veh, n =8; Cis, n =7; EA, n =8; PA38-L, n =8; PA38-H, n =7). Data are mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001 vs Veh; # P < 0.05, ## P < 0.01 vs Cis.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.714244/full'>34552585</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ajtr0011-2925-f1.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Renal RAS, oxidative stress, inflammation and glomerulosclerosis were up-regulated in DM rats. A. Representative photographs and semiquantitative data of AGT, AT1 and MCP-1 expression detected by immunohistochemistry (a1) and Western blot (a2). B. Glomerulosclerosis index measured by PAS. C. Protein level of Noxs in renal cortex measured by Western blot. Data are expressed as the mean ± SD (n=15 in each group). * P <0.05 versus non-DM rats. PAS, periodic acid-Schiff.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fimmu-12-714244-g006.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Electroacupuncture restores hematopoiesis in cancer mice during cisplatin chemotherapy. (A) Experimental flowchart depicting the time of treatments of tumor (LLC) cells at day 0, cisplatin (C), electroacupuncture (E), and analyses of tumor volume (T) and sample collection. (B) Tumor growth curve ( n =9 per group), P values were calculated using two-way repeated-measures ANOVA. (C) Peripheral blood counts of specific subpopulation of leukocytes (leukocytes, lymphocytes: T, n =8; Cis, n =6; EA, n =8. neutrophils, monocytes, T and B lymphocytes: T, n =8; Cis, n =6; EA, n =7). (D) Analyses of hematopoietic BM cell subpopulation ( n =9 per group). (E) Quantification of PI nuclear staining of BM cells ( n =9 per group). Data are mean ± SEM, * P < 0.05, ** P < 0.01, *** P < 0.001 vs Veh; # P < 0.05, ## P < 0.01 vs Cis.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.714244/full'>34552585</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ajtr0011-2925-f3.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Localization of central AGT and AT1 receptors and Blood brain barrier permeability. A. Localization of central AGT and AT1 receptors determined by doublestaining with the antibodies against AGT or AT1 receptors (green) and the antibodies-recognized NSE or GFAP (red). NSE, neuron-specific enolase; GFAP, glial fibrillary acidic protein. B. Blood brain barrier permeability was up-regulated in DM rats (b1), but there was no significant difference in all intervention groups (b2).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g1.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Age-dependent deterioration of behavior in Cdkl5 KO mice. (A) Marble burying test in young adult Cdkl5 -/Y (n = 38) and Cdkl5 +/Y (n = 31) mice, and middle-aged Cdkl5 -/Y (n = 29) and Cdkl5 +/Y (n = 22) mice. (B) Fear conditioning paradigm, measuring time spent freezing in response to a mild footshock, upon the return to the testing chamber (context) and upon hearing the testing tone (cue) in adult Cdkl5 -/Y (n = 29) and Cdkl5 +/Y (n = 28) mice, and middle-aged Cdkl5 -/Y (n = 22) and Cdkl5 +/Y (n = 20) mice. (C) Frequency of passive rotations (rotations in which the does not perform any coordinated movement but is passively transported on the rotating apparatus) in adult Cdkl5 -/Y (n = 30) and Cdkl5 +/Y (n = 19) mice, and middle-aged Cdkl5 -/Y (n = 13) and Cdkl5 +/Y (n = 10) mice. The mean frequency of passive rotations was expressed as fold of the wild-type counterparts of the same age. (D) Percentage of time spent hind-limb clasping during a 2-min interval in adult Cdkl5 -/Y (n = 29) and Cdkl5 +/Y (n =19) mice, and middle-aged Cdkl5 -/Y (n = 27) and Cdkl5 +/Y (n = 19) mice. Values represent mean ± SEM. *p< 0.05, **p< 0.01, ***p< 0.001 (Dunn’s test after Kruskal-Wallis).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ajtr0011-2925-f6.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Expression of RAS components, NOXs and TH in brain nuclei and kidney measured by western-blot. A. Protein levels of AGT (a1) and AT1 receptors (a2) in brain nuclei measured by Western-blot. B. Protein levels of NOX2 (b1) and NOX4 (b2) in brain nuclei measured by Western-blot. C. Protein expression of TH in SFO, SON PVN (c1) and RVLM (c2) measured by western-blot. D. Protein levels of AGT, AT1, MCP-1 (d1) and Noxs (d2) in renal cortex homogenates measured by Western-blot. Data are expressed as the mean ± SD (n=6 in each group). * P <0.05 versus IG 0 mg/kg/d Los.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6556645/&orig_host=www.ncbi.nlm.nih.gov'>31217864</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-ad-12-3-764-g4.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;Cdkl5 KO mice exhibit a decrease in the number of excitatory synaptic contacts. (A, C) Representative confocal micrographs of the neuropil in layer II-III of both S1 (A) and V1 (C) cortices showing immunofluorescence staining for VGluT1 (green) and Homer1bc (red) in young adult and middleaged Cdkl5 +/Y and Cdkl5 -/Y mice. Scale bar 5 μm. Arrows point to examples of VGluT1-Homer1bc coappositions. Arrowheads point to examples of solitary VGluT1+ terminals. (B, D) Quantitative analysis of the percentage of VGluT1+ terminals juxtaposed to Homer1bc+ in cerebral cortex from young adult Cdkl5 -/Y (n = 4) and Cdkl5 +/Y (n = 5) mice, and middle-aged Cdkl5 -/Y (n = 3) and Cdkl5 +/Y (n = 4) mice. Values are represented as means ± SE. **p<0.01, ***p<0.001 (Fisher’s LSD test after two-way ANOVA<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8139207/&orig_host=www.ncbi.nlm.nih.gov'>34094641</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-fimmu-12-714244-t002.jpgAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg;The node counts between proteins with PPI.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.714244/full'>34552585</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9449-th-primary-antibodies-ihc-testing-4.pngAnti-Tyrosine Hydroxylase/TH Antibody Picoband&reg; IHC analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449). <br> Tyrosine Hydroxylase/TH was detected in a paraffin-embedded section of normal mouse brain (nomal group) and Alzheimer’s model mouse brain (model group) tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-Tyrosine Hydroxylase/TH Antibody (PB9449) overnight at 4°C. A two-step IHC kit was used for 30 minutes at 37℃,The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thbs1-picoband-trade-antibody-pb9450-boster.html2026-03-25T05:22:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9450-thbs1-primary-antibodies-wb-testing-1_1.jpgAnti-Thrombospondin/THBS1 Antibody Picoband&reg; Western blot analysis of THBS1 using anti-THBS1 antibody (PB9450). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: rat C6 whole cell lysates,<br> Lane 4: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THBS1 antigen affinity purified polyclonal antibody (Catalog # PB9450) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for THBS1 at approximately 160 kDa. The expected band size for THBS1 is at 129 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr5-picoband-trade-antibody-pb9451-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9451-1-WB-anti-tlr5-picoband-antibody.jpgAnti-TLR5 Antibody Picoband&reg; Western blot analysis of TLR5 using anti-TLR5 antibody (PB9451). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: JURKAT Whole Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR5 antigen affinity purified polyclonal antibody (Catalog # PB9451) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR5 at approximately 98KD. The expected band size for TLR5 is at 83KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9451-2-IHC-anti-tlr5-picoband-antibody.jpgAnti-TLR5 Antibody Picoband&reg; IHC analysis of TLR5 using anti-TLR5 antibody (PB9451).<br> TLR5 was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR5 Antibody (PB9451) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9451-3.pngAnti-TLR5 Antibody Picoband&reg; Flow Cytometry analysis of H-PBMC cells using anti-TLR5 antibody (PB9451).<br> Overlay histogram showing H-PBMC cells stained with PB9451 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR5 Antibody (PB9451&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr7-picoband-trade-antibody-pb9452-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9452-1_2-WB-anti-tlr7-picoband-antibody.jpgAnti-TLR7 Antibody Picoband&reg; Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: MCF-7 Whole Cell Lysate<br> Lane 2: COLO320 Whole Cell Lysate<br> Lane 3: JURKAT Whole Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9452-2_2-IHC-anti-tlr7-picoband-antibody.jpgAnti-TLR7 Antibody Picoband&reg; IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).<br> TLR7 was detected in paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9452-3_1-IHC-anti-tlr7-picoband-antibody.jpgAnti-TLR7 Antibody Picoband&reg; IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).<br> TLR7 was detected in paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9452-4_1-IHC-anti-tlr7-picoband-antibody.jpgAnti-TLR7 Antibody Picoband&reg; IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).<br> TLR7 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9452-5-IHC-anti-tlr7-picoband-antibody.jpgAnti-TLR7 Antibody Picoband&reg; IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).<br> TLR7 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9452-6-IF-anti-tlr7-picoband-antibody.jpgAnti-TLR7 Antibody Picoband&reg; IHC analysis of TLR7 using anti-TLR7 antibody (PB9452).<br> TLR7 was detected in immunocytochemical section of a549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-TLR7 Antibody (PB9452) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9452-7.pngAnti-TLR7 Antibody Picoband&reg; Flow Cytometry analysis of H-PBMC cells using anti-TLR7 antibody (PB9452).<br> Overlay histogram showing H-PBMC cells stained with PB9452 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR7 Antibody (PB9452&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-versican-picoband-trade-antibody-pb9453-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9453-1_1-WB-anti-versican-picoband-antibody.jpgAnti-Versican/VCAN Antibody Picoband&reg; Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9453). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: HEPA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9453) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 373 kDa. The expected band size for Vitronectin is at 373 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9453-2_1-IHC-anti-versican-picoband-antibody.jpgAnti-Versican/VCAN Antibody Picoband&reg; IHC analysis of Vitronectin using anti-Vitronectin antibody (PB9453). <br> Vitronectin was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Vitronectin Antibody (PB9453) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9453-ott-13-12739-g0006.jpgAnti-Versican/VCAN Antibody Picoband&reg;Differentially expressed genes after rhCCL20 treatment in ovarian cancer cells. Notes: ( A ) Heatmap of differentially expressed genes after rhCCL20 treatment in SKOV3 cells for 24 hours. ( B ) KEGG analysis of differentially expressed genes after rhCCL20 treatment. ( C, D ) qRT-PCR verification of the expression of VCAN, CDH1 and CDH2 in SKOV3 and A2780 cells after rhCCL20 treatment. ( E ) H&E staining and IHC staining of N-cadherin, E-cadherin and versican on tumor tissues from in vivo experiment. *Compared to CTRL group, P <0.05 Abbreviations: KEGG, Kyoto Encyclopedia of Genes and Genomes; CTRL, control; H&E, hematoxylin and eosin; IHC, immunohistochemical; rhCCL20, recombinant human CCL20 protein.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7738160/&orig_host=www.ncbi.nlm.nih.gov'>33335408</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9453-vcan-primary-antibodies-wb-testing-3.jpgAnti-Versican/VCAN Antibody Picoband&reg; Western blot analysis of versican/VCAN using anti-versican/VCAN Antibody (PB9453). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% milk with TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-versican/VCAN Antibody (PB9453) at 1: 500 overnight at 4℃ , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with an anti-rabbit 1 hour at room temperature. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for versican/VCAN at approximately 245 kDa. The expected band size for versican/VCAN is at 373 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vcp-picoband-trade-antibody-pb9454-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-1_2.jpgAnti-VCP Antibody Picoband&reg; Western blot analysis of VCP using anti-VCP antibody (PB9454). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human U-87MG whole cell lysates, <br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human SH-SY5Y whole cell lysates,<br> Lane 6: human K562 whole cell lysates,<br> Lane 7: human Raji whole cell lysates,<br> Lane 8: human HepG2 whole cell lysates,<br> Lane 9: rat heart tissue lysates,<br> Lane 10: rat spleen tissue lysates,<br> Lane 11: rat kidney tissue lysates,<br> Lane 12: rat liver tissue lysates,<br> Lane 13: mouse heart tissue lysates,<br> Lane 14: mouse kidney tissue lysates,<br> Lane 15: mouse liver tissue lysates,<br> Lane 16: mouse HEPA1-6 whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # PB9454) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97KD. The expected band size for VCP is at 89KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-2_1.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PB9454) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-3_1.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PB9454) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-4_1.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in paraffin-embedded section of human meningeoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PB9454) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-5_1.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PB9454) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-6_1.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PB9454) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-7.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in frozen section of mouse brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PB9454) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-8.jpgAnti-VCP Antibody Picoband&reg; IHC analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in frozen section of rat brain tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VCP Antibody (PB9454) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-9.jpgAnti-VCP Antibody Picoband&reg; IF analysis of VCP using anti-VCP antibody (PB9454). <br> VCP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-VCP Antibody (PB9454) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9454-10.jpgAnti-VCP Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-VCP antibody (PB9454).<br>Overlay histogram showing HepG2 cells stained with PB9454 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VCP Antibody (PB9454,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vdac-porin-picoband-trade-antibody-pb9455-boster.html2026-03-24T05:04:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9455-2-IHC-anti-vdac-porin-picoband-antibody.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IHC analysis of VDAC using anti-VDAC antibody (PB9455). <br> VDAC was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDAC Antibody (PB9455) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9455-vdac1-primary-antibodies-wb-testing-1.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; Western blot analysis of VDAC using anti-VDAC antibody (PB9455). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat heart tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse heart tissue lysates,<br> Lane 8: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDAC antigen affinity purified polyclonal antibody (Catalog # PB9455) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDAC at approximately 35 kDa. The expected band size for VDAC is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9455-3-IHC-anti-vdac-porin-picoband-antibody.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IHC analysis of VDAC using anti-VDAC antibody (PB9455). <br> VDAC was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDAC Antibody (PB9455) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9455-4-IHC-anti-vdac-porin-picoband-antibody.jpgAnti-VDAC/Porin/VDAC1 Antibody Picoband&reg; IHC analysis of VDAC using anti-VDAC antibody (PB9455). <br> VDAC was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDAC Antibody (PB9455) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vdr-picoband-trade-antibody-pb9456-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9456-1-WB-anti-vdr-nr1i1-picoband-antibody.jpgAnti-Vitamin D Receptor/VDR Antibody Picoband&reg; Western blot analysis of VDR using anti-VDR antibody (PB9456). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug,<br> Lane 2: Rat Kidney Tissue Lysate at 50ug,<br> Lane 3: Rat Liver Tissue Lysate at 50ug,<br> Lane 4: Rat Pancreas Tissue Lysate at 50ug,<br> Lane 5: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDR antigen affinity purified polyclonal antibody (Catalog # PB9456) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDR at approximately 48 kDa. The expected band size for VDR is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9456-2-IHC-anti-vdr-nr1i1-picoband-antibody.jpgAnti-Vitamin D Receptor/VDR Antibody Picoband&reg; IHC analysis of VDR using anti-VDR antibody (PB9456). <br> VDR was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDR Antibody (PB9456) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9456-3-IHC-anti-vdr-nr1i1-picoband-antibody.jpgAnti-Vitamin D Receptor/VDR Antibody Picoband&reg; IHC analysis of VDR using anti-VDR antibody (PB9456). <br> VDR was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDR Antibody (PB9456) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9456-4-IHC-anti-vdr-nr1i1-picoband-antibody.jpgAnti-Vitamin D Receptor/VDR Antibody Picoband&reg; IHC analysis of VDR using anti-VDR antibody (PB9456). <br> VDR was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VDR Antibody (PB9456) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-villin-picoband-trade-antibody-pb9457-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9457-1-WB-anti-villin-picoband-antibody.jpgAnti-Villin/VIL1 Antibody Picoband&reg; Western blot analysis of Villin using anti-Villin antibody (PB9457). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Intestine Tissue Lysate at 50ug<br> Lane 2: Mouse Kidney Tissue Lysate at 50ug<br> Lane 3: RH35 Whole Cell Lysate at 40ug<br> Lane 4: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 5: MCF-7 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Villin antigen affinity purified polyclonal antibody (Catalog # PB9457) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Villin at approximately 93 kDa. The expected band size for Villin is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9457-2-IHC-anti-villin-picoband-antibody.jpgAnti-Villin/VIL1 Antibody Picoband&reg; IHC analysis of Villin using anti-Villin antibody (PB9457). <br> Villin was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Villin Antibody (PB9457) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-fmicb-11-622354-g001.jpgAnti-Villin/VIL1 Antibody Picoband&reg;Effects of the B. subtilis -fermented milk on the disease severity and the pathological changes in the small intestine and the colon of the DSS-induced IBD. (A) The DAI dynamic changes of the DSS-induced IBD animal models. During the second 7-day period (active phase of IBD), DAI in the DSS + B. subtilis -fermented milk group was lower than those of the DSS group and the DSS + milk group ( n = 15, p < 0.01). In the third 7-day period (recovery phase of IBD), after termination of DSS intake, DAI recovery with varying degrees was observed in the DSS group, the DSS + milk group, and the DSS + B. subtilis -fermented milk group. However, in the DSS + B. subtilis -fermented milk group, the DAI index recovered faster and was significantly lower than those of the DSS group and the DSS + milk group ( n = 15, p < 0.01). The DSS + milk group showed no obvious difference from the DSS group ( n = 15, p > 0.05). (B) The colonic appearance of the model animals at 7 days after termination of DSS administration. (C) Quantification analysis of the length of the colons in different groups. At 7 days after the termination of DSS intake, the colons in the DSS group and the DSS + milk group were shortened significantly compared with those in the normal group and B. subtilis -fermented milk group. However, in the DSS + B. subtilis -fermented milk, the colons were shortened only slightly and were significantly longer than those of the DSS group and the DSS + milk group. The B. subtilis -fermented milk group showed no obvious difference from the normal group, and the DSS + milk group showed no obvious difference from the DSS group ( n = 15, * represents p < 0.05, ** represents p < 0.01). (D,E) Changes in the histological structure of the small intestinal mucosa in the active phase of the DSS-induced IBD observed with HE staining and Alcian blue staining, respectively. (D1,E1) The normal group: the villi in the small intestinal mucosa were finger-like, which were arranged compactly and tidily. The crypt structure was integrated among the base of villi. The surface of the villi and the crypt were covered with epithelial cells and goblet cells, with abundant mucus; (D2,E2) the DSS group: the necrosis and ulcer of the mucosa were observed, the villi were disintegrated, and only some residual crypts were observed; (D3,E3) the DSS + B. subtilis -fermented milk group: damage in the small intestinal mucosa was relatively slight, and the crypt structure was almost integrated. (F,G) Changes in the histological structure of the small intestinal mucosa in the recovery phase of the DSS-induced IBD observed with HE staining and Alcian blue staining, respectively. (F1,G1) The normal group: no significant change was observed in the mucosa; (F2,G2) the DSS group: the small intestinal mucosa was partially recovered, and the villi were short and scattered; (F3,G3) the DSS + B. subtilis -fermented milk group: the intestinal mucosa epithelium and the crypts were integrated, although the regenerative villi were shorter than those of the normal small intestines. (H,I) Changes in the histological structure of the colonic mucosa in the active phase of the DSS-induced IBD observed with HE staining and Alcian blue staining, respectively. (H1,I1) The normal group: the colonic mucus were intact; (H2,I2) the DSS group: the deep ulcer was observed due to the damage of the epithelium and glands; (H3,I3) the DSS + B. subtilis -fermented milk group: fewer bleeding and necrosis were observed. The damage of the colonic mucosa was relatively slight, and only local superficial ulcers were observed. A major part of the epithelium and glands was integrated in the structure, and the goblet cells were filled with abundant mucus. (J,K) Changes in the histological structure of the colonic mucosa in the recovery phase of the DSS-induced IBD observed with HE staining and Alcian blue staining, respectively. (J1,K1) The normal group: no significant change was observed in the colonic mucosa; (J2,K2) the DSS group: ulcer of the colonic mucosa was replaced by an inflammatory scar; (J3,K3) the DSS + B. subtilis -fermented milk group: the epithelium was almost integrated and the surficial ulcer was replaced by regenerated regenerative colonic glands consisting of goblet cells, which were filled with abundant mucus. (L) Histological damage scores of different groups evaluated with H&E-stained sections of the small intestines. The histological damage scores of the DSS + B. subtilis -fermented milk group in both the active phase and the recovery phase were significantly lower than those of the DSS group ( n = 5, * represents p < 0.05). (M) Histological damage scores of different groups evaluated with H&E-stained sections of the colons. The histological damage scores of the DSS + B. subtilis -fermented milk group in both the active phase and the recovery phase were significantly lower than those of the DSS group ( n = 5, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-fmicb-11-622354-g004.jpgAnti-Villin/VIL1 Antibody Picoband&reg;The subcellular localization and relative expression level detection of ZO-1 and villin in the intestinal mucosa of IBD at 7 days after termination of DSS intake. (A) The ZO-1 immunohistochemistry staining of the small intestinal epithelial TJP (brown dots): (A1) the normal group: the villi and crypts were arranged compactly, and the ZO-1-positive staining (representing the TJP) showed the dotted line (brown) along the surface of the villi and the crypts; (A2) the DSS group: ZO-1 distributed dispersively in the residual villi of the small intestinal mucosa; (A3) the DSS + B. subtilis -fermented milk group: the ZO-1 staining formed the dotted line (brown, representing the TJP) at the subsurface of the regenerative villi. (B) The ZO-1 immunohistochemistry staining of the colonic epithelial TJP (brown dots): (B1) the normal group: ZO-1-positive staining distributed on the inner side of the epithelial cell membrane (representing the TJP); (B2) the DSS group: there was no ZO-1-positive staining in the scar; (B3) the DSS + B. subtilis -fermented milk group: the ZO-1-positive staining distributed on the inner side of the regenerative epithelial cell membrane (representing the TJP). (C) The villin immunohistochemistry staining (brown strip) of the small intestinal microvilli: (C1) the normal group: villin-positive staining showed a strip-like distribution on the surface of the villi in the normal small intestinal mucosa; (C2) the DSS group: villin distributed at the surface of the residual villi; (C3) the DSS + B. subtilis -fermented milk group: villin-positive staining formed an integrative strip (brown) enclosing the surface of the regenerative villi. (D) The villin immunohistochemistry staining of the colonic epithelium: (D1) the normal group: villin-positive staining (brown) showed banded distribution on the surface of the epithelium; (D2) the DSS group: almost no villin-positive staining was observed in the scar due to damage of the epithelium; (D3) the DSS + B. subtilis -fermented milk group: the villin-positive staining (brown) showed banded distribution on the surface of the regenerated epithelium in the colonic mucosa. (E,F) The western blotting analysis for the relative expression level of ZO-1 and villin in the samples contained equivalent ileum and colon. The expression level of ZO-1 and villin in the DSS group was significantly lower than that of the normal (control) group. The expression level of ZO-1 and villin and in the DSS + B. subtilis -fermented milk (FM) group was significantly higher than that of the DSS group ( n = 5, * represents p < 0.05, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-fmicb-11-622354-g002.jpgAnti-Villin/VIL1 Antibody Picoband&reg;The infiltration of MPO + neutrophils, and the cellular distribution and relative expression level detection of the TNF and IL-10 in the small intestinal and colonic mucosa at 7 days after the termination of DSS administration. (A) The MPO immunohistochemistry staining of the small intestinal mucosa: (A1) the normal group: few neutrophils were observed in the small intestinal mucosa; (A2) the DSS group: a number of accumulative MPO + neutrophils (brown) infiltrated into the mucosa epithelium; (A3) the DSS + B. subtilis- fermented milk group: only limited neutrophil infiltration could be observed in the small intestinal mucosa. (B) The MPO immunohistochemistry staining of the colonic mucosa: (B1) the normal group: few neutrophils were observed in the colonic mucosa; (B2) the DSS group: colonic epithelium and the glands disappeared, and the ulcer was locally replaced by scars and a number of accumulative MPO + neutrophils (brown) were observed in the scars; (B3) the DSS + B. subtilis -fermented milk group: only limited MPO + neutrophils observed in the colonic mucosa. (C) The TNF immunohistochemistry staining of the small intestinal mucosa: (C1) the normal group: the epithelium was integrated with faint yellow staining, suggesting low expression of TNF; (C2) the DSS group: the villus structure is not integrated, and the epithelial cells showed black brown, suggesting overexpression of TNF; (C3) the DSS + B. subtilis -fermented milk group: the villus and the glands were almost integrated, and the staining of epithelial cells was similar to that of the normal group (C1) , suggesting low expression of TNF. (D) The TNF immunohistochemistry staining of the colonic mucosa: (D1) the normal colonic mucosa: the epithelium was integrated with low TNF expression (faint yellow); ( D2 ) the DSS group: the epithelium structure and the glands were destroyed and replaced by a scar, and there were a number of TNF + inflammatory cells (black brown) in the scar; (D3) the DSS + B. subtilis -fermented milk group: the recovered epithelium showed faint yellow, suggesting low TNF expression. (E) The IL-10 immunohistochemistry staining of the small intestinal mucosa: (E1) the normal small intestinal mucosa: the IL-10 staining dispersed in the villi and the crypts with faint yellow, suggesting low-level expression of IL-10; (E2) the DSS group, the residual epithelium and the crypts were light brown, suggesting mid-level of IL-10 expression; (E3) the DSS + B. subtilis -fermented milk group: the dark brown staining of the regenerative epithelium represented high-level expression of IL-10. (F) The IL-10 immunohistochemistry staining of the colonic mucosa: (F1) the normal group: the IL-10 staining dispersed in the glands with bright yellow, suggesting low-level expression of IL-10; (F2) the DSS group: there were few IL-10 + cells in the scars; (F3) the DSS + B. subtilis -fermented milk group, the dark brown staining of the epithelial cells represented high-level expression of IL-10. (G,H) Western blotting analysis for the expression of MPO, TNF, and IL-10 in the samples containing equivalent ileum and colon. The expression level of MPO, TNF, and IL-10 in the DSS group was significantly higher than that of the normal (control) group. The expression level of MPO and TNF in the DSS + B. subtilis -fermented milk (FM) group was significantly lower than that of the DSS group, while the expression level of IL-10 in the DSS + B. subtilis -fermented milk (FM) group was significantly higher than that of the DSS group ( n = 5, * represents p < 0.05, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-fmicb-11-622354-g003.jpgAnti-Villin/VIL1 Antibody Picoband&reg;The distribution of Lgr5 + ISCs in the intestinalmucosa and the subcellular localization and relative expression level detection of epithelial function proteins CDX2 and villin in the intestinal mucosa of IBD at 7 days after termination of DSS administration. (A) The Lgr5 + ISCs (brown) in the small intestinal mucosa: (A1) the normal group, the villi and the crypts were arranged compactly, and Lgr5 + ISCs were observed in the crypts; (A2) the DSS group, the villi and the crypts were scattered, with few Lgr5 + ISCs; (A3) the DSS + B. subtilis -fermented milk group, there were more Lgr5 + ISCs in villi and crypts compared with those in the DSS group. (B) The Lgr5 + ISCs (brown) in the colonic mucosa: (B1) the normal group, the glands were arranged compactly, and there were large amounts of Lgr5 + ISCs at the bottom of the glands; (B2) the DSS group: the ulcers were replaced by scars. No Lgr5 + ISCs were observed in the scars; (B3) the DSS + B. subtilis -fermented milk group: the colonic epithelium was integrated, with some regenerated glands. A number of Lgr5 + ISCs were observed at the bottom of the regenerated glands. (C) The CDX2 was localized in the epithelial cellular nuclei (brown) by immunohistochemistry staining in the small intestinal mucosa: (C1) the normal group: the villi and the crypts were arranged compactly, and CDX2 + epithelial cells were observed on the surface of the villi and the crypts; (C2) the DSS group: the villi and the crypts were scattered, and few CDX2 + epithelial cells were observed on the surface of the crypt and the villi; (C3) the DSS + B. subtilis -fermented milk group: more villi and crypts were observed in comparison with the DSS group, and there were more CDX2 + epithelial cells covering the villi and crypts. (D) The CDX2 was localized in the epithelial cellular nuclei (brown) by immunohistochemistry staining in the colonic mucosa: (D1) the normal group: the colonic glands were arranged compactly, and CDX2 + epithelial cells were observed on the surface of the glands; (D2) the DSS group: the glands were scattered, and few CDX2 + epithelial cells were observed in the scar; (D3) the DSS + B. subtilis -fermented milk group: more colonic glands were observed in comparison with the DSS group, and there were more CDX2 + epithelial cells in the glands. (E) The Mucin2 was localized in the cytoplasm of the goblet cells (brown) by immunohistochemistry staining in the small intestinal mucosa: (E1) the normal group, a number of Mucin2 + goblet cells observed in the epithelium; (E2) the DSS group: only few Mucin2 + goblet cells were observed in the remaining villi and crypts; (E3) the DSS + B. subtilis -fermented milk group: more Mucin2 + goblet cells were observed in the recovered mucosa. (F) The Mucin2 was localized in the cytoplasm of the goblet cells (brown) by immunohistochemistry staining in the colonic mucosa: (F1) the normal group, large amounts of Mucin2 + goblet cells were observed in the mucosa; (F2) the DSS group: only few Mucin2 + goblet cells were observed in the scars; (F3) the DSS + B. subtilis -fermented milk group: more Mucin2 + goblet cells were observed in the recovered colonic mucosa. (G,H) Western blotting was applied for detection of the relative expression level of Lgr5, CDX2, and Mucin2 in the samples containing equivalent ileum and colon. The expression level of Lgr5, CDX2, and Mucin2 in the DSS group was significantly lower than that of the normal (control) group. The expression level of Lgr5, CDX2, and Mucin2 in the DSS + B. subtilis -fermented milk (FM) group was significantly higher than that of the DSS group ( n = 5, ** represents p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.622354/full'>33519783</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9457-3-IHC-anti-villin-picoband-antibody.jpgAnti-Villin/VIL1 Antibody Picoband&reg; IHC analysis of Villin using anti-Villin antibody (PB9457). <br> Villin was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Villin Antibody (PB9457) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9457-4-IHC-anti-villin-picoband-antibody.jpgAnti-Villin/VIL1 Antibody Picoband&reg; IHC analysis of Villin using anti-Villin antibody (PB9457). <br> Villin was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Villin Antibody (PB9457) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9457-5.jpgAnti-Villin/VIL1 Antibody Picoband&reg; IF analysis of Villi using anti-Villi antibody (PB9457) <br>Villi was detected in paraffin-embedded section of human rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Villi Antibody (PB9457) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9457-6.jpgAnti-Villin/VIL1 Antibody Picoband&reg; IF analysis of Villi using anti-Villi antibody (PB9457) <br>Villi was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Villi Antibody (PB9457) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9457-7.jpgAnti-Villin/VIL1 Antibody Picoband&reg; IF analysis of Villi using anti-Villi antibody (PB9457) <br>Villi was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Villi Antibody (PB9457) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-villin-primary-antibodies-if-testing-8_1.pngAnti-Villin/VIL1 Antibody Picoband&reg; IF analysis of Villin using anti-Villin antibody (PB9457). <br> Villin was detected in paraffin-embedded section of human ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Villin Antibody (PB9457) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-villin-primary-antibodies-if-testing-9.pngAnti-Villin/VIL1 Antibody Picoband&reg; IF analysis of Villin using anti-Villin antibody (PB9457). <br> Villin was detected in paraffin-embedded section of human colon organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Villin Antibody (PB9457) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-villin-primary-antibodies-if-testing-10.pngAnti-Villin/VIL1 Antibody Picoband&reg; IF analysis of Villin using anti-Villin antibody (PB9457). <br> Villin was detected in paraffin-embedded section of mouse ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Villin Antibody (PB9457) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-villin-primary-antibodies-if-testing-11.pngAnti-Villin/VIL1 Antibody Picoband&reg; IF analysis of Villin using anti-Villin antibody (PB9457). <br> Villin was detected in paraffin-embedded section of mouse ileum organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Villin Antibody (PB9457) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9457-villin-primary-antibodies-fcm-testing-12.jpgAnti-Villin/VIL1 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-Villin antibody (PB9457). <br>Overlay histogram showing CACO-2 cells stained with PB9457 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Villin Antibody (PB9457, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vitronectin-picoband-trade-antibody-pb9458-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9458-vitronectin-primary-antibodies-wb-testing-1.jpgAnti-Vitronectin/VTN Antibody Picoband&reg;Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9458). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (PB9458) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 65-70 kDa. The expected band size for Vitronectin is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9458-vitronectin-primary-antibodies-wb-testing-2.jpgAnti-Vitronectin/VTN Antibody Picoband&reg;Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9458). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse plamsa lysates,<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (PB9458) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 65-70 kDa. The expected band size for Vitronectin is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9458-vitronectin-primary-antibodies-ihc-testing-1.jpgAnti-Vitronectin/VTN Antibody Picoband&reg;IHC analysis of Vitronectin using anti-Vitronectin antibody (PB9458). <br>Vitronectin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitronectin Antibody (PB9458) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9458-vitronectin-primary-antibodies-ihc-testing-2.jpgAnti-Vitronectin/VTN Antibody Picoband&reg;IHC analysis of Vitronectin using anti-Vitronectin antibody (PB9458). <br>Vitronectin was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitronectin Antibody (PB9458) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9458-vitronectin-primary-antibodies-ihc-testing-3.jpgAnti-Vitronectin/VTN Antibody Picoband&reg;IHC analysis of Vitronectin using anti-Vitronectin antibody (PB9458). <br>Vitronectin was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Vitronectin Antibody (PB9458) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wasp-picoband-trade-antibody-pb9459-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9459-wasp-primary-antibodies-wb-testing-1.jpgAnti-WASP Antibody Picoband&reg; Western blot analysis of WASP using anti-WASP antibody (PB9459). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HL-60 whole cell lysates,<br> Lane 2: human U937 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse RAW264.7 cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WASP antigen affinity purified polyclonal antibody (Catalog # PB9459) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WASP at approximately 53 kDa. The expected band size for WASP is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9459-2-IHC-anti-wasp-picoband-antibody.jpgAnti-WASP Antibody Picoband&reg; IHC analysis of WAS using anti-WAS antibody (PB9459).<br> WAS was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-WAS Antibody (PB9459) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9459-3-IHC-anti-wasp-picoband-antibody.jpgAnti-WASP Antibody Picoband&reg; IHC analysis of WAS using anti-WAS antibody (PB9459).<br> WAS was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-WAS Antibody (PB9459) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9459-4-IF-anti-wasp-picoband-antibody.jpgAnti-WASP Antibody Picoband&reg; IHC analysis of WAS using anti-WAS antibody (PB9459).<br> WAS was detected in immunocytochemical section of a549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-WAS Antibody (PB9459) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt1-picoband-trade-antibody-pb9460-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9460-fimmu-16-1654180-g006.jpgAnti-Wnt1 Antibody Picoband&reg;FBXO45 activates the Wnt/β-catenin signaling pathway. (A) KEGG enrichment analysis. (B) Pearson correlation analysis revealing the relationship between FBXO45 and β-catenin. (C) GSEA of the FBXO45 and Wnt/β-catenin signaling pathway. (D, E) Western blot analysis assessing the impact of FBXO45 knockdown on key proteins in the Wnt/β-Catenin pathway in A2780 and HEY cells. (F) Western blot analysis of FBXO45 and WNT1 expression in three normal ovarian tissues and three OV tissues, accompanied by statistical analysis (* p < 0.05; ** p < 0.01; *** p < 0.001).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2025.1654180/full'>40990020</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9460-wnt1-primary-antibodies-wb-testing-1.jpgAnti-Wnt1 Antibody Picoband&reg;Western blot analysis of Wnt1 using anti-Wnt1 antibody (PB9460). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human CACO-2 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt1 antigen affinity purified polyclonal antibody (PB9460) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Wnt1 at approximately 45 kDa. The expected band size for Wnt1 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt2-picoband-trade-antibody-pb9461-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9461-1-WB-anti-wnt2-picoband-antibody.jpgAnti-Wnt2 Antibody Picoband&reg; Western blot analysis of WNT2 using anti-WNT2 antibody (PB9461). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: COLO320 Whole Cell Lysate at 40ug,<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT2 antigen affinity purified polyclonal antibody (Catalog # PB9461) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT2 at approximately 40 kDa. The expected band size for WNT2 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt2b-picoband-trade-antibody-pb9462-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9462-1-WB-anti-wnt2b-picoband-antibody.jpgAnti-Wnt2b Antibody Picoband&reg; Western blot analysis of WNT2B using anti-WNT2B antibody (PB9462). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: 22RV1 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT2B antigen affinity purified polyclonal antibody (Catalog # PB9462) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT2B at approximately 44 kDa. The expected band size for WNT2B is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9462-2-IHC-anti-wnt2b-picoband-antibody.jpgAnti-Wnt2b Antibody Picoband&reg; IHC analysis of WNT2B using anti-WNT2B antibody (PB9462). <br> WNT2B was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNT2B Antibody (PB9462) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9462-3-IHC-anti-wnt2b-picoband-antibody.jpgAnti-Wnt2b Antibody Picoband&reg; IHC analysis of WNT2B using anti-WNT2B antibody (PB9462). <br> WNT2B was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNT2B Antibody (PB9462) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9462-4-IHC-anti-wnt2b-picoband-antibody.jpgAnti-Wnt2b Antibody Picoband&reg; IHC analysis of WNT2B using anti-WNT2B antibody (PB9462). <br> WNT2B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNT2B Antibody (PB9462) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xbp1-picoband-trade-antibody-pb9463-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9463-1-WB-anti-xbp1-picoband-antibody.jpgAnti-XBP1 Antibody Picoband&reg; Western blot analysis of XBP using anti-XBP antibody (PB9463). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 2: MM231 Whole Cell Lysate at 40ug,<br> Lane 3: MM453 Whole Cell Lysate at 40ug,<br> Lane 4: SKOV Whole Cell Lysate at 40ug,<br> Lane 5: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XBP antigen affinity purified polyclonal antibody (Catalog # PB9463) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XBP at approximately 29 kDa. The expected band size for XBP is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9463-2-IHC-anti-xbp1-picoband-antibody.jpgAnti-XBP1 Antibody Picoband&reg; IHC analysis of XBP using anti-XBP antibody (PB9463). <br> XBP was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XBP Antibody (PB9463) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9463-fphys-13-941706-g005.jpgAnti-XBP1 Antibody Picoband&reg;Inhibition of autophagic flux alleviated hepatocyte ER stress and LD accumulation. (A) Changes in ER morphology and LD accumulation in different groups were assessed. (B) Hepatic XBP1, IRE1α, and EIF2α protein levels were compared among groups. (C) XBP1 and IRE1α protein levels were normalized based on β-Actin levels, and the p-EIF2α/EIF2α ratio was compared among groups. (Yellow arrow: LDs; black arrow: ER; * p < 0.05, ** p < 0.01, ns: no statistical difference).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.941706/full?utm_source=dlvr.it&utm_medium=twitter'>35982710</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9463-3-IHC-anti-xbp1-picoband-antibody.jpgAnti-XBP1 Antibody Picoband&reg; IHC analysis of XBP using anti-XBP antibody (PB9463). <br> XBP was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XBP Antibody (PB9463) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9463-4-IHC-anti-xbp1-picoband-antibody.jpgAnti-XBP1 Antibody Picoband&reg; IHC analysis of XBP using anti-XBP antibody (PB9463). <br> XBP was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XBP Antibody (PB9463) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9463-5.pngAnti-XBP1 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-XBP1 antibody (PB9463). <br> Overlay histogram showing HepG2 cells stained with PB9463 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XBP1 Antibody (PB9463&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ku80-picoband-trade-antibody-pb9464-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9464-ku80-primary-antibodies-wb-testing-1.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; Western blot analysis of KU80 using anti-KU80 antibody (PB9464). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KU80 antigen affinity purified polyclonal antibody (Catalog # PB9464) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKU80 at approximately 83 kDa. The expected band size for KU80 is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9464-2-IHC-anti-ku80-picoband-antibody.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IHC analysis of KU80 using anti-KU80 antibody (PB9464). <br> KU80 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KU80 Antibody (PB9464) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9464-3.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IHC analysis of Ku80 using anti-Ku80 antibody (PB9464). <br> Ku80 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ku80 Antibody (PB9464) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9464-4.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; IF analysis of Ku80 using anti-Ku80 antibody (PB9464). <br> Ku80 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Ku80 Antibody (PB9464) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9464-5.jpgAnti-Ku80/XRCC5 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-Ku80 antibody (PB9464).<br>Overlay histogram showing SiHa cells stained with PB9464 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ku80 Antibody (PB9464,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-yb1-picoband-trade-antibody-pb9465-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9465-ybx1-primary-antibodies-wb-testing-1.jpgAnti-YB1/YBX1 Antibody Picoband&reg; Western blot analysis of YB1 using anti-YB1 antibody (PB9465). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human T47D whole cell lysates, <br> Lane 5: rat spleen tissue lysates, <br> Lane 6: mouse RAW264.7 whole cell lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YB1 antigen affinity purified polyclonal antibody (Catalog # PB9465) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YB1 at approximately 50 kDa. The expected band size for YB1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9465-2_1-IHC-anti-yb1-picoband-antibody.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YB using anti-YB antibody (PB9465).<br> YB was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-YB Antibody (PB9465) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9465-3_1-IHC-anti-yb1-picoband-antibody.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YB using anti-YB antibody (PB9465).<br> YB was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-YB Antibody (PB9465) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9465-4_1-IHC-anti-yb1-picoband-antibody.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YB using anti-YB antibody (PB9465).<br> YB was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-YB Antibody (PB9465) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9465-5_1-IHC-anti-yb1-picoband-antibody.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IHC analysis of YB using anti-YB antibody (PB9465).<br> YB was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-YB Antibody (PB9465) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9465-ybx1-primary-antibodies-if-testing-6.jpgAnti-YB1/YBX1 Antibody Picoband&reg; IF analysis of YB1 using anti-YB1 antibody (PB9465). <br> YB1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-YB1 Antibody (PB9465) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9465-ybx1-primary-antibodies-fcm-testing-7_1.pngAnti-YB1/YBX1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-YB1 antibody (PB9465). <br> Overlay histogram showing A431 cells stained with PB9465 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YB1 Antibody (PB9465, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-yes1-picoband-trade-antibody-pb9466-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9466-yes1-primary-antibodies-wb-testing-1.jpgAnti-Yes1 Antibody Picoband&reg; Western blot analysis of YES1 using anti-YES1 antibody (PB9466). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U20S whole cell lysates, <br> Lane 2: human CACO-2 whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: human MCF-7 whole cell lysates, <br> Lane 6: human PC-3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YES1 antigen affinity purified polyclonal antibody (Catalog # PB9466) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YES1 at approximately 61 kDa. The expected band size for YES1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9466-2-IHC-anti-yes1-yes-picoband-antibody.jpgAnti-Yes1 Antibody Picoband&reg; IHC analysis of YES1 using anti-YES1 antibody (PB9466). <br> YES1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-YES1 Antibody (PB9466) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-actin-antibody-rp1070-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-wb-testing-1.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; Western blot analysis of Actin using anti-Actin antibody (RP1070). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: human A431 whole cell lysates,<br> Lane 6: human U87 whole cell lysates,<br> Lane 7: human U937 whole cell lysates,<br> Lane 8: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (Catalog # RP1070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-wb-testing-2.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; Western blot analysis of Actin using anti-Actin antibody (RP1070). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: mouse heart tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse spleen tissue lysates,<br> Lane 7: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (Catalog # RP1070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-ihc-testing-3.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (RP1070). <br> Actin was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (RP1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-ihc-testing-4.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (RP1070). <br> Actin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (RP1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-ihc-testing-5.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (RP1070). <br> Actin was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (RP1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-ihc-testing-6.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (RP1070). <br> Actin was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (RP1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-ihc-testing-7.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (RP1070). <br> Actin was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (RP1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1070-actin-primary-antibodies-ihc-testing-8.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (RP1070). <br> Actin was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (RP1070) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-agfg1-antibody-rp1071-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1071-agfg1-primary-antibodies-wb-testing-1.jpgAnti-AGFG1 Antibody Picoband&reg; Western blot analysis of AGFG1 using anti-AGFG1 antibody (RP1071). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGFG1 antigen affinity purified polyclonal antibody (RP1071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AGFG1 at approximately 58 kDa. The expected band size for AGFG1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1071-2.jpgAnti-AGFG1 Antibody Picoband&reg; IF analysis of AGFG1 using anti-AGFG1 antibody (RP1071). <br> AGFG1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AGFG1 Antibody (RP1071) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1071-3.jpgAnti-AGFG1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-AGFG1 antibody (RP1071).<br>Overlay histogram showing SiHa cells stained with RP1071 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGFG1 Antibody (RP1071,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sp1-antibody-rp1072-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1072-sp1-primary-antibodies-wb-testing-1.jpgAnti-Transcription factor Sp1 SP1 Antibody Picoband&reg; Western blot analysis of SP1 using anti-SP1 antibody (RP1072). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human CACO-2 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human COLO320 whole lysates, <br> Lane 5: rat testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP1 antigen affinity purified polyclonal antibody (Catalog # RP1072) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP1 at approximately 90 kDa. The expected band size for SP1 is at 90 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tlr8-antibody-rp1073-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1073-1-WB-anti-tlr8-antibody.jpgAnti-Toll-like receptor 8 TLR8 Antibody Picoband&reg;Anti-TLR8 antibody&#44; RP1073&#44; Western blotting<br>All lanes: Anti TLR8 (RP1073) at 0.5ug/ml<br>Lane 1: Rat Liver Tissue Lysate at 50ug<br>Lane 2: HEPG2 Whole Cell Lysate at 40ug<br>Predicted bind size: 120KD<br>Observed bind size: 120KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-abl-picoband-trade-antibody-pb9468-boster.html2026-03-24T05:05:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9468-1_2-WB-anti-c-abl-picoband-antibody.jpgAnti-c Abl/ABL1 Antibody Picoband&reg; Western blot analysis of cABI using anti-cABI antibody (PB9468). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cABI antigen affinity purified polyclonal antibody (Catalog # PB9468) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cABI at approximately 150 kDa. The expected band size for cABI is at 123 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rage-picoband-trade-antibody-pb9469-boster.html2026-03-28T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9469-1-WB-anti-rage-picoband-antibody.jpgAnti-RAGE/AGER Antibody Picoband&reg; Western blot analysis of RAGE using anti-RAGE antibody (PB9469). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Lung Tissue Lysate at 50ug,<br> Lane 2: RH35 Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAGE antigen affinity purified polyclonal antibody (Catalog # PB9469) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAGE at approximately 45-58 kDa. The expected band size for RAGE is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9469-2-IHC-anti-rage-picoband-antibody.jpgAnti-RAGE/AGER Antibody Picoband&reg; IHC analysis of RAGE using anti-RAGE antibody (PB9469). <br> RAGE was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RAGE Antibody (PB9469) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9469-3-IHC-anti-rage-picoband-antibody.jpgAnti-RAGE/AGER Antibody Picoband&reg; IHC analysis of RAGE using anti-RAGE antibody (PB9469). <br> RAGE was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RAGE Antibody (PB9469) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9469-4-IHC-anti-rage-picoband-antibody.jpgAnti-RAGE/AGER Antibody Picoband&reg; IHC analysis of RAGE using anti-RAGE antibody (PB9469). <br> RAGE was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RAGE Antibody (PB9469) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-agtr1-picoband-trade-antibody-pb9470-boster.html2026-03-25T05:22:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9470-agtr1-primary-antibodies-wb-testing-1.jpgAnti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband&reg; Western blot analysis of AGTR1 using anti-AGTR1 antibody (PB9470). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates, <br> Lane 2: human T-47D whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human U-87MG whole cell lysates, <br> Lane 5: human HepG2 whole cell lysates, <br> Lane 6: monkey COS-7 whole cell lysates, <br> Lane 7: rat brain tissue lysates, <br> Lane 8: rat liver tissue lysates, <br> Lane 9: rat kidney tissue lysates, <br> Lane 10: rat RH35 whole cell lysates, <br> Lane 11: mouse brain tissue lysates, <br> Lane 12: mouse liver tissue lysates, <br> Lane 13: mouse kidney tissue lysates, <br> Lane 14: mouse HEPA1-6 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (Catalog # PB9470) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9470-2-IHC-anti-agtr1-picoband-antibody.jpgAnti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband&reg; IHC analysis of AGTR1 using anti-AGTR1 antibody (PB9470). <br> AGTR1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AGTR1 Antibody (PB9470) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh2-picoband-trade-antibody-pb9472-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9472-aldh2-primary-antibodies-wb-testing-1.jpgAnti-ALDH2 Antibody Picoband&reg;Western blot analysis of ALDH2 using anti-ALDH2 antibody (PB9472). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: rat liver tissuelysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat kidney tissue lysates,<br> Lane 5: mouse kidney tissue lysates,<br> Lane 6: mouse ANA-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH2 antigen affinity purified polyclonal antibody (PB9472) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9472-aldh2-primary-antibodies-wb-testing-2.pngAnti-ALDH2 Antibody Picoband&reg;Western blot analysis of ALDH2 using anti-ALDH2 antibody (PB9472). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Control group-mouse hippocampus tissue, <br> Lane 2: Model group-mouse hippocampus tissue, <br> Lane 3: Drug treatment (100 mg/kg) – Mouse hippocampus tissue, <br> Lane 4: : Drug treatment (100 mg/kg) – Mouse hippocampus tissue.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH2 antigen affinity purified polyclonal antibody (PB9472) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9472-aldh2-primary-antibodies-ihc-testing-1.jpgAnti-ALDH2 Antibody Picoband&reg;IHC analysis of ALDH2 using anti-ALDH2 antibody (PB9473). <br> ALDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH2 Antibody (PB9473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9472-aldh2-primary-antibodies-fcm-testing-1.jpgAnti-ALDH2 Antibody Picoband&reg;Flow Cytometry analysis of HepG2 cells using anti-ALDH2 antibody (PB9472). <br> Overlay histogram showing HepG2 cells stained with PB9472 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH2 Antibody (PB9472, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9472-fnut-09-848918-g005.jpgAnti-ALDH2 Antibody Picoband&reg;Effects of WEATs on ALDH2 expression in the liver of mice. Representative immunoblots (A) and IHC images (B) of ALDH2 expression in the liver. Quantification of ALDH2 expression by western blotting (C) and IHC (D) . Each value represents the mean ± SEM ( n = 9). * p < 0.05 vs. MOD; ** p < 0.01 vs. MOD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9169692/&orig_host=www.ncbi.nlm.nih.gov'>35677547</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-1-picoband-trade-antibody-pb9473-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-ihc-testing-5.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg;IHC analysis of AQP1 using anti-AQP1 antibody (PB9473). <br>AQP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PB9473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-ihc-testing-6.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg;IHC analysis of AQP1 using anti-AQP1 antibody (PB9473). <br>AQP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PB9473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-fphar-15-1469223-g004.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg;Immunoprecipitation determinations of HMGB1 and AQP1 (A) Western blots of HMGB1 in plasmids. (B) Western blots of AQP1 in plasmids. (C) CO-IP assay results. 293T: 293T-null cells; 293T-E5061-E5077: 293T- E5061 HA empty control plasmid transfection- E5077 negative control CON238 plasmid; 293T-E5062-E5078: 293T- E5062 HA-Aqp1 overexpression plasmid transfection- E5078 Hmgb1-3flag overexpression plasmid. Flag: HMGB1; HA:AQP1.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1469223/full'>39359252</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-fphar-15-1469223-g005.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg;Changes in AQP1 expression after HMGB1 knockdown (A) Protein expression levels of HMGB1 and AQP1 in the spinal cords of rats in each group. (B) Protein expression levels of HMGB1 and AQP1 in the LPS inflammatory cell model in each group. (C) mRNA levels of HMGB1 and AQP1 in the spinal cords of rats in each group. (D) mRNA levels of HMGB1 and AQP1 in the LPS inflammatory cell model in each group. N = 3 per group **** p < 0.0001 *** p < 0.001 ** p < 0.01* p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1469223/full'>39359252</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-fphar-15-1469223-g006.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg;Changes in AQP1 expression after TAK-242 and FPS-ZM1 treatments (A) Protein expression levels of TLR4 and AQP1 in the spinal cords of rats in each group. (B) Protein expression levels of TLR4 and AQP1 in the LPS inflammatory cell model in each group. (C) Protein expression levels of RAGE and AQP1 in the spinal cords of rats in each group. (D) Protein expression levels of RAGE and AQP1 in the LPS inflammatory cell model in each group. (E) mRNA levels of TLR4 and AQP1 in each group. (F) mRNA levels of RAGE and AQP1 in each group. N = 3 per group **** p < 0.0001 *** p < 0.001 ** p < 0.01* p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1469223/full'>39359252</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-fphar-15-1469223-g007.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg;Changes in NF-κB expression following knockdown of HMGB1 or AQP1 (A) Protein expression levels of HMGB1 and NF-κB in the spinal cords of rats in each group. (B) mRNA levels of HMGB1 and NF-κB in the spinal cords of rats in each group. (C) Protein expression levels of AQP1 and NF-κB in the spinal cords of rats in each group. (D) mRNA levels of AQP1 and NF-κB in the spinal cords of rats in each group. (E) Protein expression levels of NF-κB and AQP1 in the LPS inflammatory cell model in each group. (F) mRNA levels of NF-κB and AQP1 in the LPS inflammatory cell model in each group. N = 3 per group **** p < 0.0001 *** p < 0.001 ** p < 0.01* p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1469223/full'>39359252</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-wb-testing-1_1.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; Western blot analysis of AQP1 using anti-AQP1 antibody (PB9473). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates, <br> Lane 2: rat kidney tissue lysates, <br> Lane 3: rat lung tissue lysates, <br> Lane 4: mouse heart tissue lysates, <br> Lane 5: mouse kidney tissue lysates, <br> Lane 6: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP1 antigen affinity purified polyclonal antibody (PB9473) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AQP1 at approximately 25, 35-38 kDa. The expected band size for AQP1 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-ihc-testing-2_1.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; IHC analysis of AQP1 using anti-AQP1 antibody (PB9473). <br> AQP1 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PB9473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-ihc-testing-3_1.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; IHC analysis of AQP1 using anti-AQP1 antibody (PB9473). <br> AQP1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PB9473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-ihc-testing-4_1.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; IHC analysis of AQP1 using anti-AQP1 antibody (PB9473). <br> AQP1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AQP1 Antibody (PB9473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-if-testing-5.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; IF analysis of AQP1 using anti-AQP1 antibody (PB9473). <br> AQP1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-AQP1 Antibody (PB9473) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9473-aqp1-primary-antibodies-fcm-testing-6.jpgAnti-Aquaporin 1/AQP1 Antibody Picoband&reg; Flow Cytometry analysis of U2OS cells using anti-AQP1 antibody (PB9473). <br> Overlay histogram showing U2OS cells stained with PB9473 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP1 Antibody (PB9473, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-2-picoband-trade-antibody-pb9474-boster.html2026-03-25T05:22:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9474-aqp2-primary-antibodies-wb-testing-1.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg; Western blot analysis of Aquaporin 2 using anti-Aquaporin 2 antibody (PB9474). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: rat NRK whole cell lysates, <br> Lane 3: rat PC-12 whole cell lysates, <br> Lane 4: mouse kidney tissue lysates, <br> Lane 5: mouse HBZY whole cell lysates, <br> Lane 6: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 2 antigen affinity purified polyclonal antibody (Catalog # PB9474) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 2 at approximately 26 kDa. The expected band size for Aquaporin 2 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9474-2-IHC-anti-aquaporin-2-picoband-antibody.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg; IHC analysis of Aquaporin 2 using anti-Aquaporin 2 antibody (PB9474). <br> Aquaporin 2 was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 2 Antibody (PB9474) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9474-3-IHC-anti-aquaporin-2-picoband-antibody.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg; IHC analysis of Aquaporin 2 using anti-Aquaporin 2 antibody (PB9474). <br> Aquaporin 2 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 2 Antibody (PB9474) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9474-4-IHC-anti-aquaporin-2-picoband-antibody.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg; IHC analysis of Aquaporin 2 using anti-Aquaporin 2 antibody (PB9474). <br> Aquaporin 2 was detected in paraffin-embedded section of Human Renal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 2 Antibody (PB9474) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9474-aqp2-primary-antibodies-fc-testing-5.jpgAnti-Aquaporin 2/AQP2 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-AQP2 antibody (PB9474). <br>Overlay histogram showing PC-3 cells stained with PB9474 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AQP2 Antibody (PB9474&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-4-picoband-trade-antibody-pb9475-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-12974_2024_3030_fig7_html.pngAnti-Aquaporin 4/AQP4 Antibody Picoband&reg;Effects of maternal obesity on the hippocampal vascularization and coverage of capillaries by end-feet of astrocytes in offspring: no significant difference in the length density of CD31 + capillaries between two groups of mice ( A ); an example of the length density of AQP4 + capillaries measurement. The green lines (arrow) represent the intersection between isotropic virtual plane and the focal plane. Capillaries that are in focus and intersected by virtual planes are counted as positive AQP4.For estimating the reference volume, we use the four corner points of the box.Scale bar = 20 μm ( B ); a significant higher coverage of capillaries with end-feet of astrocytes ( A ) without changes in vascularization in offspring of obese dams ( C ); a significant positive correlation between length density of AQP4 + capillaries and number of astrocytic branches ( D ); a significant higher endothelial expression of LCN2, suggesting longer LCN2 + capillaries, in the hippocampus of offspring of obese dams ( E ); examples of hippocampal endothelial LCN2 expression in the offspring of control ( F ) and obese dams ( G ). Scale bar = 20 μm; a significant positive correlation between length density of LCN2 + capillaries and number of astrocytic branches ( H ); a significant negative correlation between length density of LCN2 + capillaries and volume of hippocampus ( I ). * p < 0.05, ** p < 0.01, ( n = 6/group) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12974-024-03030-w'>38308309</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-12974_2024_3305_fig3_html.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg;CD22 blockade exacerbates NMOSD pathology in mice. A T2WI scans revealed demyelinating lesions in the indicated groups of NMOSD mice. The lesion areas are marked with red lines. Scale bar: 2 mm. B Bar graphs depicting the volume of demyelinating lesions in the indicated groups of NMOSD mice; n = 10 per group. C Immunostaining of the indicated markers (GFAP, AQP4, or MBP) in brain tissue sections from NMOSD mice receiving the anti-CD22 mAb or IgG control on day 3 after NMOSD induction. The white lines indicate areas with loss of AQP4, GFAP or MBP. Scale bar: 3,000 μm (left), 100 μm (right). D Bar graphs illustrating demyelination in NMOSD mice receiving anti-CD22 mAb or IgG control; n = 10 per group. The data are expressed as the mean ± SEM. ** p < 0.01<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11607829/'>39616380</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-thnov15p4495g006.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg;T cells, B cells and NK cells are not involved in the benefit of FPR2 antagonism in NMOSD mice. ( A ) 9.4T-T2 weighted MR images showing brain lesions within the axial position in RAG2 -/- NMOSD mice treated with vehicle or Quin-C7 (32 mg/kg/day). ( B ) Bar graph showing the volumes of intracranial lesions in RAG2 -/- NMOSD mice treated with vehicle or Quin-C7 (32 mg/kg/day). ( C ) Schematic diagram depicts experimental design. NMOSD was induced in NK depleted mice by intracerebral injection of AQP4-IgG with hC. Thereafter, these mice received daily administration of Quin-C7 (32 mg/kg) or an equal volume of vehicle for 4 consecutive days starting immediately after NMOSD induction. On day 4 after NMOSD, the imaging analysis was performed. For NK cell depletion, mice were injected i.p. with 250 μg anti-NK1.1 mAb or isotype control every three days, the first time was injected one day before NMOSD induction. ( D-E ) Flow cytometry gating strategy ( D ) and quantification of NK cell percentage ( E ) in the peripheral blood of mice with or without anti-NK1.1 mAb treatment. n = 6 per group. ( F ) Representative 9.4T-T2 weighted MR images showing brain lesions within the axial position in indicated groups of NMOSD mice. ( G ) The histogram shows the volumes of intracranial lesions in indicated groups of NMOSD mice. Data are presented as mean ± SEM. ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11984409/'>40225578</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-thnov15p4495g005.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg;Microglia contribute to the benefit of FPR2 antagonism in NMOSD mice. ( A ) Schematic diagram illustrates drug administration and experimental design. C57BL/6 mice received oral gavage of PLX5622 for 14 days and continuously until the experiment ended. NMOSD was induced in PLX5622-treated mice by intracerebral injection of AQP4-IgG with hC. Thereafter, these mice received daily administration of Quin-C7 (32 mg/kg) or an equal volume of vehicle for 4 consecutive days starting immediately after NMOSD induction. On day 4 after NMOSD, the imaging analysis was performed. ( B-C ) Flow cytometry gating strategy ( B ) and bar graph ( C ) showing microglia percentage in the brain tissues obtained from mice receiving a control diet or PLX5622 for 14 days. n = 6 per group. ( D ) 9.4T-T2 weighted MR images showing brain lesions within the axial position in indicated groups of NMOSD mice. ( E ) Bar graph showing the volumes of intracranial lesions in indicated groups of NMOSD mice. Data are presented as mean ± SEM. ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11984409/'>40225578</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-aqp4-primary-antibodies-wb-testing-1.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; Western blot analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4 antigen affinity purified polyclonal antibody (Catalog # PB9475) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 4 at approximately 37 kDa. The expected band size for Aquaporin 4 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-thnov15p4495g003.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg;FPR2 antagonism reduces NMOSD pathology in mice. ( A ) Schematic diagram depicts experimental design. Mice received daily gavage administration of different dosages of Quin-C7 or an equal volume of vehicle for four consecutive days, starting immediately after NMOSD induction. Mouse brain tissue was collected for immunostaining analysis on day 4 post-NMOSD. ( B ) Immunostaining shows the expression of AQP4, GFAP, and MBP in indicated groups of NMOSD mice, white lines represent the area with the loss of AQP4, GFAP and MBP. Scale bar: 1mm. ( C ) Bar graphs showing the loss of AQP4, GFAP and MBP. n = 8-11 mice per group. ( D ) 9.4T-T2 weighted MR images showing brain lesions within the axial position in vehicle or Quin-C7-treated (32 mg/kg/day) NMOSD mice on day 4. ( E ) Bar graph showing the intracranial lesion volumes of the vehicle or Quin-C7-treated (32 mg/kg/day) NMOSD mice. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11984409/'>40225578</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-aqp4-primary-antibodies-ihc-testing-2.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475). <br> Aquaporin 4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-aqp4-primary-antibodies-ihc-testing-3.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475). <br> Aquaporin 4 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-aqp4-primary-antibodies-wb-testing-2_2_.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg;Western blot analysis of AQP4 using anti-AQP4 antibody (PB9475). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions. <br> Lane 1: Zebrafish head tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP4 antigen affinity purified polyclonal antibody (PB9475) at 0.5 μg/ml overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AQP4 at approximately 37 kDa.The expected band size for AQP4 is at 37 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-aqp4-primary-antibodies-ihc-testing-4.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475). <br> Aquaporin 4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9475-5-IHC-anti-aquaporin-4-picoband-antibody.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475). <br> Aquaporin 4 was detected in frozen section of Mouse Brain Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9475-6-IHC-anti-aquaporin-4-picoband-antibody.jpgAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; IHC analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475). <br> Aquaporin 4 was detected in frozen section of Rat Brain Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Aquaporin 4 Antibody (PB9475) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9475-aqp4-primary-antibodies-wb-testing-3.pngAnti-Aquaporin 4/AQP4 Antibody Picoband&reg; Western blot analysis of Aquaporin 4 using anti-Aquaporin 4 antibody (PB9475). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Normal group-rat colon tissue lysates,<br> Lane 2: Model group-rat colon tissue lysates,<br> Lane 3: Traditional Chinese medicine-rat colon tissue lysates,<br> Lane 4: Western medicine-rat colon tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 4 antigen affinity purified polyclonal antibody (Catalog # PB9475) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Aquaporin 4 at approximately 37 kDa. The expected band size for Aquaporin 4 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aplp1-picoband-trade-antibody-pb9476-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9476-1_3-WB-anti-aplp1-picoband-antibody.jpgAnti-APLP1 Antibody Picoband&reg; Western blot analysis of APLP1 using anti-APLP1 antibody (PB9476). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44;<br> Lane 2: Rat Testis Tissue Lysate&#44;<br> Lane 3: SGC Whole Cell Lysate&#44;<br> Lane 4: 22RV1 Whole Cell Lysate&#44;<br> Lane 5: MCF-7 Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APLP1 antigen affinity purified polyclonal antibody (Catalog # PB9476) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APLP1 at approximately 85KD. The expected band size for APLP1 is at 72KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9476-aplp1-primary-antibodies-ihc-testing-4.jpgAnti-APLP1 Antibody Picoband&reg;IHC analysis of APLP1 using anti-APLP1 antibody (PB9476). <br>APLP1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-APLP1 Antibody (PB9476) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9476-2_2-IHC-anti-aplp1-picoband-antibody.jpgAnti-APLP1 Antibody Picoband&reg; IHC analysis of APLP1 using anti-APLP1 antibody (PB9476). <br> APLP1 was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APLP1 Antibody (PB9476) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9476-3_2-IHC-anti-aplp1-picoband-antibody.jpgAnti-APLP1 Antibody Picoband&reg; IHC analysis of APLP1 using anti-APLP1 antibody (PB9476). <br> APLP1 was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-APLP1 Antibody (PB9476) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9476-aplp1-primary-antibodies-fc-testing-4.jpgAnti-APLP1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-APLP1 antibody (PB9476). <br>Overlay histogram showing SiHa cells stained with PB9476 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APLP1 Antibody (PB9476&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arsa-picoband-trade-antibody-pb9477-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9477-arsa-primary-antibodies-wb-testing-1.jpgAnti-ARSA Antibody Picoband&reg;Western blot analysis of ARSA using anti-ARSA antibody (PB9477). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: rat kidney tissue lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse kidney tissue lysates,<br> Lane 7: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARSA antigen affinity purified polyclonal antibody (PB9477) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARSA at approximately 54 kDa. The expected band size for ARSA is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9477-2-IHC-anti-arsa-arylsulfatase-a-picoband-antibody.jpgAnti-ARSA Antibody Picoband&reg; IHC analysis of ARSA using anti-ARSA antibody (PB9477).<br> ARSA was detected in paraffin-embedded section of Rat Lung Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARSA Antibody (PB9477) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9477-3-IHC-anti-arsa-arylsulfatase-a-picoband-antibody.jpgAnti-ARSA Antibody Picoband&reg; IHC analysis of ARSA using anti-ARSA antibody (PB9477).<br> ARSA was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARSA Antibody (PB9477) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9477-arsa-primary-antibodies-fc-testing-4_1.jpgAnti-ARSA Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-ARSA antibody (PB9477).<br>Overlay histogram showing Hela cells stained with PB9477 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (PB9477,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9477-arsa-primary-antibodies-fc-testing-5.jpgAnti-ARSA Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-ARSA antibody (PB9477).<br>Overlay histogram showing PC-3 cells stained with PB9477 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARSA Antibody (PB9477&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9477-arsa-primary-antibodies-if-testing-6_1.jpgAnti-ARSA Antibody Picoband&reg; IF analysis of ARSA using anti-ARSA antibody (PB9477).<br> ARSA was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ARSA Antibody (PB9477) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-asph-picoband-trade-antibody-pb9478-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9478-1-WB-anti-asph-aspartate-beta-hydroxylase-picoband-antibody.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg; Western blot analysis of ASPH using anti-ASPH antibody (PB9478). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44;<br> Lane 2: Rat Liver Tissue Lysate&#44;<br> Lane 3: HELA Whole Cell Lysate&#44;<br> Lane 4: HEPG2 Whole Cell Lysate&#44;<br> Lane 5: HEPA Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASPH antigen affinity purified polyclonal antibody (Catalog # PB9478) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASPH at approximately 100KD. The expected band size for ASPH is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-jcav15p1138g001.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg;Immunoblotting detection of the ASPH protein. TC-1, TC-1/dASPH, TC-1/A9 and MK16/KLL cells were treated with 20 μM MO-I-1151 inhibitor for 24 h and DMSO was used as a control. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. Antibodies applied for ASPH staining are indicated.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10861829/&orig_host=www.ncbi.nlm.nih.gov'>38356711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-jcav15p1138g002.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg;The effect of ASPH inhibition on cell proliferation. (A) TC-1, TC-1/dASPH, TC-1/A9 and MK16/KLL cells were treated with the MO-I-1151 inhibitor at concentration 0.1, 1, 5, 10 and 20 μM for 48 h and then subjected to an MTT assay or (B) incubated with MO-I-1151 at 20 μM for 7 days, stained with crystal violet and photographed. Images were quantified by ImageJ software. DMSO was used as a control. Data represents the mean ± SEM of three independent experiments. Statistical significance refers to the comparison with the non-treated (DMSO) samples. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001 by t -test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10861829/&orig_host=www.ncbi.nlm.nih.gov'>38356711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-jcav15p1138g003.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg;The effect of ASPH inhibition on cell migration. (A) Confluent TC-1, TC-1/dASPH, TC-1/A9 and MK16/KLL cells were incubated with 20 μM MO-I-1151 for the indicated times. The migration was measured by the area of the wound made in cells and images were taken at 0, 6 and 12 h and images were quantified using ImageJ software. (B) Cell lines were treated with 20 μM MO-I-1151 in transwell chambers. After 24 h, the cells were fixed and stained. Microscopic images were quantified by ImageJ software. DMSO was used as a control. Data represents the mean ± SEM of three independent experiments. Statistical significance refers to the comparison with the non-treated (DMSO) samples. * p <0.05 , ** p <0.01 by t -test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10861829/&orig_host=www.ncbi.nlm.nih.gov'>38356711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-jcav15p1138g004.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg;The effect of ASPH inhibition on cell invasions. (A) Spheroids were embedded into a 3D collagen matrix and treated with 20 μM MO-I-1151. Images were taken at 0 and 72 h and quantified by ImageJ software. (B) Cell lines were treated with 20 μM MO-I-1151 in pre-coated Matrigel transwell chambers. After 24 h, the cells were fixed and stained. Microscopic images were quantified by ImageJ software. DMSO was used as a control. Data represents the mean ± SEM of three independent experiments. Statistical significance refers to the comparison with the non-treated (DMSO) samples. * p <0.05, ** p <0.01 by t -test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10861829/&orig_host=www.ncbi.nlm.nih.gov'>38356711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-jcav15p1138g005.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg;The effect of ASPH inhibition on cellular signaling. Cells were treated with 20 μM MO-I-1151 for 24 h (DMSO was used as a control), and protein samples were collected and subjected to SDS-PAGE electrophoresis and immunoblotting. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. (A) Notch and SRC pathways, (B) epithelial-mesenchymal pathway.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10861829/&orig_host=www.ncbi.nlm.nih.gov'>38356711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-jcav15p1138g006.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg;The effect of ASPH downregulation on gene expression. Bulk RNA-seq was performed after ASPH inhibition with 20 μM MO-I-1151 inhibitor for 24 h in TC-1, TC-1/A9, and MK16/KLL cells or ASPH deactivation with CRISPR/Cas9 in TC-1/dASPH cells (n=3). DMSO was used as a control . (A) Differential gene expression. Orange dots indicate genes with at least 2-fold decreased or increased expression and p adj ≤0.01. Numbers of these genes are indicated in green and red colors, respectively. (B) Overlap of down- and upregulated genes. (C) Enrichment analysis. Some of the most significant differences found with Hallmark and Reactome gene sets are shown.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10861829/&orig_host=www.ncbi.nlm.nih.gov'>38356711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-jcav15p1138g007.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg;The effect of ASPH inhibition on regulation of Ly6 family members. Cells were treated with 20 μM MO-I-1151 inhibitor for 24 h and DMSO was used as a control. (A) Ly6a and Ly6c1 expression was detected by RT-qPCR and relative quantification was calculated. Data represents the mean ± SEM of three independent experiments. TC-1, TC-1/A9, and MK16/KLL cell lines incubated with MO-I-1151 were compared with DMSO-treated controls and TC-1/dASPH cells with TC-1 cell line. * p <0.05, ** p <0.01 by t -test. (B, C, D) Ly6 proteins were examined by immunoblotting. Equal amounts of proteins were subjected to SDS-PAGE. GAPDH was used as an internal control. Ly6a and Ly6c were detected in cell lines TC-1/dASPH, TC-1, TC-1/A9, and MK16/KLL (B) and RMA, JUN-3, 4T1, and B16-F10 (C) and Ly6D and Ly6K in human cell lines HeLa, CaSki, MCF-7, Detroit 562 and SiHa (D) .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10861829/&orig_host=www.ncbi.nlm.nih.gov'>38356711</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9478-2-IHC-anti-asph-aspartate-beta-hydroxylase-picoband-antibody.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg; IHC analysis of ASPH using anti-ASPH antibody (PB9478). ASPH was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ASPH Antibody (PB9478) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9478-3.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg; IHC analysis of ASPH using anti-ASPH antibody (PB9478).<br> ASPH was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-ASPH Antibody ( PB9478) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9478-4.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg; Flow Cytometry analysis of HeLa cells using anti-ASPH antibody (PB9478). <br>Overlay histogram showing HeLa cells stained with PB9478 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASPH Antibody (PB9478&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9478-5.jpgAnti-Aspartate beta hydroxylase/ASPH Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-ASPH antibody (PB9478). <br>Overlay histogram showing U87 cells stained with PB9478 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ASPH Antibody (PB9478&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apg7-picoband-trade-antibody-pb9479-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9479-1-WB-anti-apg7-picoband-antibody.jpgAnti-Apg7/ATG7 Antibody Picoband&reg; Western blot analysis of APG7 using anti-APG7 antibody (PB9479). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: 293T Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APG7 antigen affinity purified polyclonal antibody (Catalog # PB9479) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APG7 at approximately 78 kDa. The expected band size for APG7 is at 78 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kiaa0652-picoband-trade-antibody-pb9480-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9480-1-WB-anti-kiaa0652-picoband-antibody.jpgAnti-KIAA0652/ATG13 Antibody Picoband&reg; Western blot analysis of KIAA0652 using anti-KIAA0652 antibody (PB9480). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA0652 antigen affinity purified polyclonal antibody (Catalog # PB9480) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA0652 at approximately 56 kDa. The expected band size for KIAA0652 is at 56 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atg14l-picoband-trade-antibody-pb9481-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9481-1-WB-anti-atg14l-picoband-antibody.jpgAnti-ATG14L Antibody Picoband&reg; Western blot analysis of ATG14L using anti-ATG14L antibody (PB9481). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44;<br> Lane 2: HELA Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG14L antigen affinity purified polyclonal antibody (Catalog # PB9481) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG14L at approximately 59KD. The expected band size for ATG14L is at 59KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9481-2-IHC-anti-atg14l-picoband-antibody.jpgAnti-ATG14L Antibody Picoband&reg; IHC analysis of ATG14L using anti-ATG14L antibody (PB9481). <br> ATG14L was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATG14L Antibody (PB9481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9481-3-IHC-anti-atg14l-picoband-antibody.jpgAnti-ATG14L Antibody Picoband&reg; IHC analysis of ATG14L using anti-ATG14L antibody (PB9481). <br> ATG14L was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATG14L Antibody (PB9481) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9481-atg14l-primary-antibodies-if-testing-4.jpgAnti-ATG14L Antibody Picoband&reg; IF analysis of ATG14L using anti-ATG14L antibody (PB9481). <br> ATG14L was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ATG14L Antibody (PB9481) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9481-atg14l-primary-antibodies-if-testing-5.jpgAnti-ATG14L Antibody Picoband&reg; IF analysis of ATG14L using anti-ATG14L antibody (PB9481). <br> ATG14L was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATG14L Antibody (PB9481) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9481-atg14l-primary-antibodies-fc-testing-8.jpgAnti-ATG14L Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-ATG14L antibody (PB9481). <br>Overlay histogram showing SiHa cells stained with PB9481 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (PB9481&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9481-atg14l-primary-antibodies-if-testing-6.jpgAnti-ATG14L Antibody Picoband&reg; IF analysis of ATG14L using anti-ATG14L antibody (PB9481). <br> ATG14L was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATG14L Antibody (PB9481) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9481-atg14l-primary-antibodies-fc-testing-9.jpgAnti-ATG14L Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ATG14L antibody (PB9481). <br>Overlay histogram showing A431 cells stained with PB9481 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (PB9481&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9481-atg14l-primary-antibodies-if-testing-7.jpgAnti-ATG14L Antibody Picoband&reg; IF analysis of ATG14L using anti-ATG14L antibody (PB9481). <br> ATG14L was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATG14L Antibody (PB9481) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9481-atg14l-primary-antibodies-fc-testing-10.jpgAnti-ATG14L Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-ATG14L antibody (PB9481). <br>Overlay histogram showing PC-3 cells stained with PB9481 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (PB9481&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atp1a1-picoband-trade-antibody-pb9482-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-wb-testing-1_1.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; Western blot analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates, <br> Lane 4: mouse brain tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A1 antigen affinity purified polyclonal antibody (Catalog # PB9482) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody (Left) at a dilution of 1:2000 or a goat anti-rabbit IgG-HRP Conjugated secondary antibody (Right) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A1 approximately 100 kDa. The expected band size for ATP1A1 is at 74, 110, 113 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-2.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-3.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-4.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-5.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-6.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-7.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-8.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-ihc-testing-9.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg; IHC analysis of ATP1A1 using anti-ATP1A1 antibody (PB9482). <br> ATP1A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATP1A1 Antibody (PB9482) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-if-testing-1.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg;IF analysis of ATP1A1 using anti-ATP1A1 antibody (A05223-3). <br> ATP1A1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATP1A1 Antibody (A05223-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9482-atp1a1-primary-antibodies-if-testing-2.jpgAnti-alpha 1 Sodium Potassium ATPase/ATP1A1 Antibody Picoband&reg;IF analysis of ATP1A1 using anti-ATP1A1 antibody (A05223-3). <br> ATP1A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ATP1A1 Antibody (A05223-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atx2-picoband-trade-antibody-pb9483-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9483-atxn2-primary-antibodies-wb-testing-1.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; Western blot analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: mouse brain tissue lysates, <br> Lane 6: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATX2/ATXN2 antigen affinity purified polyclonal antibody (Catalog # PB9483) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATX2/ATXN2 at approximately 140 kDa. The expected band size for ATX2/ATXN2 is at 140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9483-atxn2-primary-antibodies-ihc-testing-2.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483). <br> ATX2/ATXN2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9483-atxn2-primary-antibodies-ihc-testing-3.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483). <br> ATX2/ATXN2 was detected in a paraffin-embedded section of human colonic adenoma cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9483-atxn2-primary-antibodies-ihc-testing-4.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483). <br> ATX2/ATXN2 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9483-atxn2-primary-antibodies-ihc-testing-5.jpgAnti-ATX2/ATXN2 Antibody Picoband&reg; IHC analysis of ATX2/ATXN2 using anti-ATX2/ATXN2 antibody (PB9483). <br> ATX2/ATXN2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ATX2/ATXN2 Antibody (PB9483) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bak-picoband-trade-antibody-pb9484-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9484-3-IHC-anti-bak-picoband-antibody.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484). <br> BAK was detected in paraffin-embedded section of Rat Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-wb-testing-1.jpgAnti-BAK/BAK1 Antibody Picoband&reg;Western blot analysis of BAK using anti-BAK antibody (PB9484). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: human Hacat whole cell lysates,<br> Lane 6: human A431 whole cell lysates,<br> Lane 7: human Caco-2 whole cell lysates,<br> Lane 8: human RT4 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK antigen affinity purified polyclonal antibody (PB9484) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BAK at approximately 23 kDa. The expected band size for BAK is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9484-4-IHC-anti-bak-picoband-antibody.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484). <br>BAK was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-wb-testing-2.jpgAnti-BAK/BAK1 Antibody Picoband&reg;Western blot analysis of BAK using anti-BAK antibody (PB9484). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: rat heart tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse thymus tissue lysates,<br> Lane 6: mouse heart tissue lysates,<br> Lane 7: mouse lung tissue lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK antigen affinity purified polyclonal antibody (PB9484) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BAK at approximately 23 kDa. The expected band size for BAK is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-ihc-testing-5.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484).<br> BAK was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-ihc-testing-6.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484).<br> BAK was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-ihc-testing-7.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484).<br> BAK was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-ihc-testing-8.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484).<br> BAK was detected in frozen section of mouse cardiac muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-ihc-testing-9.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484).<br> BAK was detected in frozen section of rat cardiac muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9484-2-IHC-anti-bak-picoband-antibody.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IHC analysis of BAK using anti-BAK antibody (PB9484). <br>BAK was detected in paraffin-embedded section of Mouse Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BAK Antibody (PB9484) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-if-testing-12.jpgAnti-BAK/BAK1 Antibody Picoband&reg; IF analysis of BAK using anti-BAK antibody (PB9484). <br> BAK was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-BAK Antibody (PB9484) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9484-bak-primary-antibodies-fc-testing-11.pngAnti-BAK/BAK1 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-BAK antibody (PB9484). <br> Overlay histogram showing THP-1 cells stained with PB9484 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAK Antibody (PB9484&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-a1-picoband-trade-antibody-pb9485-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9485-ccna1-primary-antibodies-wb-testing-1_1.jpgAnti-Cyclin A1/CCNA1 Antibody Picoband&reg;Western blot analysis of Cyclin A1/CCNA1 using anti-Cyclin A1/CCNA1 antibody (PB9485). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: rat kidney tissue lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: mouse kidney tissue lysates, <br> Lane 7: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A1/CCNA1 antigen affinity purified polyclonal antibody (Catalog # PB9485) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cyclin A1/CCNA1 at approximately 52 kDa. The expected band size for Cyclin A1/CCNA1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9485-ccna1-primary-antibodies-if-testing-2_1.jpgAnti-Cyclin A1/CCNA1 Antibody Picoband&reg;IF analysis of Cyclin A1/CCNA1 using anti-Cyclin A1/CCNA1 antibody (PB9485) and anti-Tubulin Alpha antibody (M03989-3).<br> Cyclin A1/CCNA1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cyclin A1/CCNA1 Antibody (PB9485) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9485-ccna1-primary-antibodies-fcm-testing-1.jpgAnti-Cyclin A1/CCNA1 Antibody Picoband&reg;Flow Cytometry analysis of PC-3 cells using anti-Cyclin A1/CCNA1 antibody (PB9485). <br> Overlay histogram showing PC-3 cells stained with PB9485 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin A1/CCNA1 Antibody (PB9485, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd46-picoband-trade-antibody-pb9486-boster.html2026-03-24T05:05:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9486-cd46-primary-antibodies-wb-testing-1.jpgAnti-CD46 Antibody Picoband&reg; Western blot analysis of CD46 using anti-CD46 antibody (PB9486). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD46 antigen affinity purified polyclonal antibody (Catalog # PB9486) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD46 at approximately 50-70 kDa. The expected band size for CD46 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9486-cd46-primary-antibodies-ihc-testing-2.jpgAnti-CD46 Antibody Picoband&reg; IHC analysis of CD46 using anti-CD46 antibody (PB9486).<br> CD46 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD46 Antibody (PB9486) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9486-cd46-primary-antibodies-ihc-testing-3.jpgAnti-CD46 Antibody Picoband&reg; IHC analysis of CD46 using anti-CD46 antibody (PB9486).<br> CD46 was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD46 Antibody (PB9486) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9486-cd46-primary-antibodies-if-testing-4.jpgAnti-CD46 Antibody Picoband&reg; IF analysis of CD46 using anti- CD46 antibody (PB9486). <br> CD46 was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CD46 Antibody (PB9486) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd55-picoband-trade-antibody-pb9487-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9487-cd55-primary-antibodies-fcm-testing-3.jpgAnti-CD55 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-CD55 antibody (PB9487). <br> Overlay histogram showing PC-3 cells stained with PB9487 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD55 Antibody (PB9487&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9487-cd55-primary-antibodies-fcm-testing-4.jpgAnti-CD55 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-CD55 antibody (PB9487). <br> Overlay histogram showing SiHa cells stained with PB9487 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD55 Antibody (PB9487&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9487-cd55-primary-antibodies-wb-testing-1_1_1.jpgAnti-CD55 Antibody Picoband&reg; Western blot analysis of CD55 using anti-CD55 antibody (PB9487). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD55 antigen affinity purified polyclonal antibody (Catalog # PB9487) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD55 at approximately 75KD. The expected band size for CD55 is at 75KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9487-cd55-primary-antibodies-ihc-testing-2_1.jpgAnti-CD55 Antibody Picoband&reg; IHC analysis of CD55 using anti-CD55 antibody (PB9487). <br> CD55 was detected in paraffin-embedded section of Human Placenta Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD55 Antibody (PB9487) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9487-cd55-primary-antibodies-if-testing-5.jpgAnti-CD55 Antibody Picoband&reg; IF analysis of CD55 using anti-CD55 antibody (PB9487). <br> CD55 was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD55 Antibody (PB9487) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9487-cd55-primary-antibodies-if-testing-6.jpgAnti-CD55 Antibody Picoband&reg; IF analysis of CD55 using anti-CD55 antibody (PB9487). <br> CD55 was detected in paraffin-embedded section of huamn placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-CD55 Antibody (PB9487) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc25b-picoband-trade-antibody-pb9488-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9488-cdc25b-primary-antibodies-wb-testing-1.jpgAnti-Cdc25B Antibody Picoband&reg; Western blot analysis of Cdc25B using anti-Cdc25B antibody (PB9488). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25B antigen affinity purified polyclonal antibody (Catalog # PB9488) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25B at approximately 65 kDa. The expected band size for Cdc25B is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9488-cdc25b-primary-antibodies-ihc-testing-2.jpgAnti-Cdc25B Antibody Picoband&reg; IHC analysis of Cdc25B using anti-Cdc25B antibody (PB9488). <br> Cdc25B was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cdc25B Antibody (PB9488) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9488-cdc25b-primary-antibodies-if-testing-3.jpgAnti-Cdc25B Antibody Picoband&reg; IF analysis of Cdc25B using anti-Cdc25B antibody (PB9488). <br> Cdc25B was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cdc25B Antibody (PB9488) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9488-cdc25b-primary-antibodies-fcm-testing-4.jpgAnti-Cdc25B Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Cdc25B antibody (PB9488). <br> Overlay histogram showing HepG2 cells stained with PB9488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cdc25B Antibody (PB9488, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cib1-picoband-trade-antibody-pb9489-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9489-perilipin-a-primary-antibodies-wb-testing-1.jpgAnti-CIB1 Antibody Picoband&reg; Western blot analysis of CIB1 using anti-CIB1 antibody (PB9489). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: rat PC-12 whole cell lysates,<br> Lane 3: mouse brian tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIB1 antigen affinity purified polyclonal antibody (Catalog # PB9489) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIB1 at approximately 22 kDa. The expected band size for CIB1 is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-jab1-picoband-trade-antibody-pb9490-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9490-cops5-primary-antibodies-wb-testing-1.jpgAnti-JAB1/COPS5 Antibody Picoband&reg; Western blot analysis of JAB1 using anti-JAB1 antibody (PB9490). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human A549 whole cell lysates, <br> Lane 6: human HEK293 whole cell lysates, <br> Lane 7: rat brain tissue lysates, <br> Lane 8: rat heart tissue lysates, <br> Lane 9: mouse brain tissue lysates, <br> Lane 10: mouse heart tissue lysates, <br> Lane 11: mouse SP2/0 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JAB1 antigen affinity purified polyclonal antibody (Catalog # PB9490) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JAB1 at approximately 38 kDa. The expected band size for JAB1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9490-2-IHC-anti-jab1-picoband-antibody.jpgAnti-JAB1/COPS5 Antibody Picoband&reg; IHC analysis of JAB1 using anti-JAB1 antibody (PB9490). <br> JAB1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-JAB1 Antibody (PB9490) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9490-3-IHC-anti-jab1-picoband-antibody.jpgAnti-JAB1/COPS5 Antibody Picoband&reg; IHC analysis of JAB1 using anti-JAB1 antibody (PB9490). <br> JAB1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-JAB1 Antibody (PB9490) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9490-4-IHC-anti-jab1-picoband-antibody.jpgAnti-JAB1/COPS5 Antibody Picoband&reg; IHC analysis of JAB1 using anti-JAB1 antibody (PB9490). <br> JAB1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-JAB1 Antibody (PB9490) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9490-5.pngAnti-JAB1/COPS5 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-JAB1 antibody (PB9490). <br> Overlay histogram showing U20S cells stained with PB9490 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAB1 Antibody (PB9490&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9490-6.pngAnti-JAB1/COPS5 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-JAB1 antibody (PB9490). <br> Overlay histogram showing U937 cells stained with PB9490 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JAB1 Antibody (PB9490&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9490-7.jpgAnti-JAB1/COPS5 Antibody Picoband&reg; ICC analysis of JAB1 using anti-JAB1 antibody (PB9490).<br> JAB1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-JAB1 Antibody (PB9490) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9490-cops5-primary-antibodies-ip-testing-1.jpgAnti-JAB1/COPS5 Antibody Picoband&reg;Immunoprecipitating (IP) JAB1 in A549 whole cell lysate.<br> Western blot analysis of JAB1 using anti-JAB1 antibody (PB9490); <br> Lane 1: A549 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-JAB1 antibody in A549 whole cell lysate;<br> Lane 3: anti-JAB1 antibody (2μg) + A549 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-JAB1 antigen affinity purified polyclonal antibody (PB9490) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for JAB1 at approximately 38 kDa. The expected band size for JAB1 is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cpt1b-picoband-trade-antibody-pb9491-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9491-cpt1b-primary-antibodies-wb-testing-1.jpgAnti-CPT1B Antibody Picoband&reg; Western blot analysis of CPT1B using anti-CPT1B antibody (PB9491). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates, <br> Lane 2: rat skeletal muscle tissue lysates, <br> Lane 3: mouse heart tissue lysates, <br> Lane 4: mouse skeletal muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1B antigen affinity purified polyclonal antibody (Catalog # PB9491) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1B at approximately 88 kDa. The expected band size for CPT1B is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9491-gr6.jpgAnti-CPT1B Antibody Picoband&reg;Expression of the lipid metabolism regulator PPARβ and downstream effectors (CPT1b, FAS, and ACC2). Protein levels in obese rats under hypoxic conditions, with hypoxic training, and with regulated miR-122 expression were determined using Western blot analysis. OE, obese rats with miR-122 overexpression and hypoxic training; IE, obese rats with miR-122 depletion and hypoxic training; CE, obese rats with hypoxic training only; H, obese sedentary rats without regulation of miR-122 expression. Data are presented as mean ± SD. *p < 0.05, **p < 0.01 vs rats in group H; # p < 0.05, ## p < 0.01 vs rats in group CE; & p < 0.05, && p < 0.01 vs rats in group OE (1-way analysis of variance). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10906430/&orig_host=www.ncbi.nlm.nih.gov'>38434053</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9491-2-IHC-anti-cpt1b-picoband-antibody.jpgAnti-CPT1B Antibody Picoband&reg; IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).<br> CPT1B was detected in paraffin-embedded section of Mouse Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9491-3-IHC-anti-cpt1b-picoband-antibody.jpgAnti-CPT1B Antibody Picoband&reg; IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).<br> CPT1B was detected in paraffin-embedded section of Rat Cardiac Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9491-cpt1b-primary-antibodies-ihc-testing-4.jpgAnti-CPT1B Antibody Picoband&reg; IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).<br> CPT1B was detected in frozen section of mouse cardiac muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9491-cpt1b-primary-antibodies-ihc-testing-5.jpgAnti-CPT1B Antibody Picoband&reg; IHC analysis of CPT1B using anti-CPT1B antibody (PB9491).<br> CPT1B was detected in frozen section of rat cardiac muscle tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9491-cpt1b-primary-antibodies-if-testing-6.jpgAnti-CPT1B Antibody Picoband&reg; IF analysis of CPT1B using anti-CPT1B antibody (PB9491). <br> CPT1B was detected in a paraffin-embedded section of mouse cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9491-cpt1b-primary-antibodies-if-testing-7.jpgAnti-CPT1B Antibody Picoband&reg; IF analysis of CPT1B using anti-CPT1B antibody (PB9491). <br> CPT1B was detected in a paraffin-embedded section of rat cardiac tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CPT1B Antibody (PB9491) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-creb3l1-picoband-trade-antibody-pb9492-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9492-1-WB-anti-creb3l1-oasis-picoband-antibody.jpgAnti-CREB3L1 Antibody Picoband&reg; Western blot analysis of CREB3L1 using anti-CREB3L1 antibody (PB9492). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug,<br> Lane 4: MM231 Whole Cell Lysate at 40ug,<br> Lane 5: COLO320 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3L1 antigen affinity purified polyclonal antibody (Catalog # PB9492) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CREB3L1 at approximately 57 kDa. The expected band size for CREB3L1 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctcf-picoband-trade-antibody-pb9493-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9493-ctcf-primary-antibodies-wb-testing-1_1.jpgAnti-CTCF Antibody Picoband&reg; Western blot analysis of CTCF using anti-CTCF antibody (PB9493). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human SiHa whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTCF antigen affinity purified polyclonal antibody (PB9493) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CTCF at approximately 150 kDa. The expected band size for CTCF is at 83 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9493-ctcf-primary-antibodies-if-testing-2.jpgAnti-CTCF Antibody Picoband&reg; IF analysis of CTCF using anti-CTCF antibody (PB9493) and anti-Tubulin Alpha antibody (M03989-3).<br> CTCF was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CTCF Antibody (PB9493) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9493-ctcf-primary-antibodies-fcm-testing-3.jpgAnti-CTCF Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-CTCF antibody (PB9493). <br> Overlay histogram showing MCF-7 cells stained with A04887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTCF Antibody (PB9493, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cystathionase-picoband-trade-antibody-pb9494-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9494-1_1.jpgAnti-Cystathionase/CTH Antibody Picoband&reg; Western blot analysis of Cystathionase using anti-Cystathionase antibody (PB9494). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> lane 1: K562 whole cell lysates,<br> lane 2: HepG2 whole cell lysates, <br> lane 3: RH35 whole cell lysates,<br> lane 4: rat liver tissue lysates,<br> lane 5: rat kidney tissue lysates,<br> lane 6: mouse liver tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystathionase antigen affinity purified polyclonal antibody (Catalog # PB9494) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystathionase at approximately 45KD. The expected band size for Cystathionase is at 45KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ica1-picoband-trade-antibody-pb9495-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9495-ica1-primary-antibodies-wb-testing-1.jpgAnti-ICA1 Antibody Picoband&reg; Western blot analysis of ICA1 using anti-ICA1 antibody (PB9495). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat pancreas tissue lysates,<br> Lane 6: rat brain tissue lysates,<br> Lane 7: mouse pancreas tissue lysates,<br> Lane 8: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICA1 antigen affinity purified polyclonal antibody (Catalog # PB9495) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ICA1 at approximately 60-65 kDa. The expected band size for ICA1 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-itch-aip4-picoband-trade-antibody-pb9496-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9496-itch-primary-antibodies-wb-testing-1.jpgAnti-ITCH/AIP4 Antibody Picoband&reg; Western blot analysis of ITCH/AIP4 using anti-ITCH/AIP4 antibody (PB9496). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: monkey COS-7 whole cell lysates,<br> Lane 5: human HepG2 whole cell lysates,<br> Lane 6: human A549 whole cell lysates,<br> Lane 7: rat C6 whole cell lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITCH/AIP4 antigen affinity purified polyclonal antibody (Catalog # PB9496) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITCH/AIP4 at approximately 103 kDa. The expected band size for ITCH/AIP4 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9496-itch-primary-antibodies-fcm-testing-2.jpgAnti-ITCH/AIP4 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-ITCH antibody (PB9496). <br> Overlay histogram showing SiHa cells stained with PB9496 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ITCH Antibody (PB9496, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nqo1-picoband-trade-antibody-pb9497-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9497-13020_2024_1027_fig6_html.jpgAnti-NQO1 Antibody Picoband&reg;RSBQD activated PI3K/AKT/Nrf2 signaling pathway in exercise fatigued mice (n = 10). A Hepatic GSH-Px activity. B Hepatic SOD activity. C Hepatic MDA content. D – F The relative mRNA levels of Sod1 ( D ), Nrf2 ( E ), and Ho-1 ( F ). G Representative images of Western blot. H – K Relative protein levels of KEAP1 ( H ), NRF2 ( I ), HO-1 ( J ), and NQO1 ( K ). ( L , M ) The ratio of p-PI3K/PI3K L and p-AKT/AKT M . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11539552/'>39501343</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9497-nqo1-primary-antibodies-wb-testing-1.jpgAnti-NQO1 Antibody Picoband&reg; Western blot analysis of NQO1 using anti-NQO1 antibody (PB9497). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: rat RH35 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (Catalog # PB9497) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 31 kDa. The expected band size for NQO1 is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pinx1-picoband-trade-antibody-pb9498-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9498-1-WB-anti-pinx1-picoband-antibody.jpgAnti-PINX1 Antibody Picoband&reg; Western blot analysis of PINX1 using anti-PINX1 antibody (PB9498). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: HUT Whole Cell Lysate at 40ug,<br> Lane 4: JURKAT Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PINX1 antigen affinity purified polyclonal antibody (Catalog # PB9498) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PINX1 at approximately 37 kDa. The expected band size for PINX1 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pklr-picoband-trade-antibody-pb9499-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9499-pklr-primary-antibodies-wb-testing-1.jpgAnti-PKLR Antibody Picoband&reg; Western blot analysis of PKLR using anti-PKLR antibody (PB9499). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKLR antigen affinity purified polyclonal antibody (Catalog # PB9499) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKLR at approximately 62 kDa. The expected band size for PKLR is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9499-2-IHC-anti-pklr-picoband-antibody.jpgAnti-PKLR Antibody Picoband&reg; IHC analysis of PKLR using anti-PKLR antibody (PB9499). <br> PKLR was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PKLR Antibody (PB9499) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-plk1-picoband-trade-antibody-pb9500-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9500-1-WB-anti-plk1-plk-picoband-antibody.jpgAnti-PLK1 Antibody Picoband&reg; Western blot analysis of PLK1 using anti-PLK1 antibody (PB9500). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug,<br> Lane 2: PANC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLK1 antigen affinity purified polyclonal antibody (Catalog # PB9500) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLK1 at approximately 68 kDa. The expected band size for PLK1 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-scarb1-picoband-trade-antibody-pb9502-boster.html2026-03-24T05:05:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9502-scarb1-primary-antibodies-wb-testing-1.jpgAnti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband&reg; Western blot analysis of SCARB1 using anti-SCARB1 antibody (PB9502). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARB1 antigen affinity purified polyclonal antibody (Catalog # PB9502) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCARB1 at approximately 85 kDa. The expected band size for SCARB1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9502-scarb1-primary-antibodies-ihc-testing-2.jpgAnti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband&reg; IHC analysis of SCARB1 using anti-SCARB1 antibody (PB9502). <br> SCARB1 was detected in a paraffin-embedded section of rat adrenal gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCARB1 Antibody (PB9502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9502-scarb1-primary-antibodies-ihc-testing-3.jpgAnti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband&reg; IHC analysis of SCARB1 using anti-SCARB1 antibody (PB9502). <br> SCARB1 was detected in a paraffin-embedded section of rat adrenal gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SCARB1 Antibody (PB9502) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sgca-picoband-trade-antibody-pb9503-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9503-1-WB-anti-sgca-alpha-sarcoglycan-picoband-antibody.jpgAnti-alpha Sarcoglycan/SGCA Antibody Picoband&reg; Western blot analysis of SGCA using anti-SGCA antibody (PB9503). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug,<br> Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA antigen affinity purified polyclonal antibody (Catalog # PB9503) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGCA at approximately 43 kDa. The expected band size for SGCA is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc19a1-picoband-trade-antibody-pb9504-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9504-1_1.jpgAnti-SLC19A1 Antibody Picoband&reg; Western blot analysis of SLC19A1 using anti-SLC19A1 antibody (PB9504). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat ovary tissue lysates&#44; <br> Lane 3: mouse brain tissue lysates&#44; <br> Lane 4: mouse testicular tissue lysates&#44; <br> Lane 5: mouse ovary tissue lysates&#44; <br> Lane 6: mouse NIH/3T3 whole cell lysates&#44; <br> Lane 7: human placenta tissue lysates&#44; <br> Lane 8: human HEK293 whole cell lysates&#44; <br> Lane 9: monkey COS-7 whole cell lysates&#44; <br> Lane 10: human PC-3 whole cell lysates&#44; <br> Lane 11: human 22RV1 whole cell lysates&#44; <br> Lane 12: human K562 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC19A1 antigen affinity purified polyclonal antibody (Catalog # PB9504) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC19A1 at approximately 65KD. The expected band size for SLC19A1 is at 65KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tcptp-picoband-trade-antibody-pb9501-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9501-1-WB-anti-tcptp-picoband-antibody.jpgAnti-TCPTP/PTPN2 Antibody Picoband&reg; Western blot analysis of TCPTP using anti-TCPTP antibody (PB9501). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug,<br> Lane 2: JURKAT Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCPTP antigen affinity purified polyclonal antibody (Catalog # PB9501) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCPTP at approximately 48 kDa. The expected band size for TCPTP is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9501-2-IHC-anti-tcptp-picoband-antibody.jpgAnti-TCPTP/PTPN2 Antibody Picoband&reg; IHC analysis of TCPTP using anti-TCPTP antibody (PB9501). <br> TCPTP was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TCPTP Antibody (PB9501) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9501-3-IHC-anti-tcptp-picoband-antibody.jpgAnti-TCPTP/PTPN2 Antibody Picoband&reg; IHC analysis of TCPTP using anti-TCPTP antibody (PB9501). <br> TCPTP was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TCPTP Antibody (PB9501) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/picokine-elisa-kits/rat-scf-picokine-trade-elisa-kit-ek0508-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0508_1.pngRat SCF/KITLG/Kit ligand ELISA Kit PicoKine®Rat SCF PicoKine ELISA Kit Standard Curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-socs4-picoband-trade-antibody-pb9506-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9506-1-WB-anti-socs4-picoband-antibody.jpgAnti-SOCS4 Antibody Picoband&reg; Western blot analysis of SOCS4 using anti-SOCS4 antibody (PB9506). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 2: SW620 Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: SKOV Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS4 antigen affinity purified polyclonal antibody (Catalog # PB9506) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS4 at approximately 51 kDa. The expected band size for SOCS4 is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sox5-picoband-trade-antibody-pb9507-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9507-1-WB-anti-sox5-picoband-antibody.jpgAnti-SOX5 Antibody Picoband&reg; Western blot analysis of SOX5 using anti-SOX5 antibody (PB9507). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Rat Testis Tissue Lysate at 50ug,<br> Lane 3: Rat Brain Tissue Lysate at 50ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug,<br> Lane 5: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody (Catalog # PB9507) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84 kDa. The expected band size for SOX5 is at 84 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9507-2-western-blotting.jpgAnti-SOX5 Antibody Picoband&reg;<b> Western blot analysis of SOX5 using anti-SOX5 antibody (PB9507). </b><br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. <br>All lanes: pig adipose cells<br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody (Catalog # PB9507) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84KD. The expected band size for SOX5 is at 84KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9507-3-western-blotting.jpgAnti-SOX5 Antibody Picoband&reg; Western blot analysis of SOX5 using anti-SOX5 antibody (PB9507). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysate&#44; <br> Lane 2: mouse thymus tissue lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody (Catalog # PB9507) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84KD. The expected band size for SOX5 is at 84KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sp3-picoband-trade-antibody-pb9508-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9508-1-WB-anti-sp3-picoband-antibody.jpgAnti-SP3 Antibody Picoband&reg; Western blot analysis of SP3 using anti-SP3 antibody (PB9508). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: PC-12 Whole Cell Lysate at 40ug,<br> Lane 2: HEPA Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP3 antigen affinity purified polyclonal antibody (Catalog # PB9508) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP3 at approximately 82 kDa. The expected band size for SP3 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9508-2-IHC-anti-sp3-picoband-antibody.jpgAnti-SP3 Antibody Picoband&reg; IHC analysis of SP3 using anti-SP3 antibody (PB9508). <br> SP3 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP3 Antibody (PB9508) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9508-3-IHC-anti-sp3-picoband-antibody.jpgAnti-SP3 Antibody Picoband&reg; IHC analysis of SP3 using anti-SP3 antibody (PB9508). <br> SP3 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP3 Antibody (PB9508) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9508-4-IHC-anti-sp3-picoband-antibody.jpgAnti-SP3 Antibody Picoband&reg; IHC analysis of SP3 using anti-SP3 antibody (PB9508). <br> SP3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SP3 Antibody (PB9508) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sfrs3-picoband-trade-antibody-pb9509-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9509-sfrs3-primary-antibodies-wb-testing-1.jpgAnti-SFRS3/SRSF3 Antibody Picoband&reg; Western blot analysis of SFRS3 using anti-SFRS3 antibody (PB9509). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human SiHa whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRS3 antigen affinity purified polyclonal antibody (Catalog # PB9509) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRS3 at approximately 19 kDa. The expected band size for SFRS3 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9509-sfrs3-primary-antibodies-if-testing-2.jpgAnti-SFRS3/SRSF3 Antibody Picoband&reg; IF analysis of SFRS3 using anti-SFRS3 antibody (PB9509) and anti-Beta Tubulin antibody (M01857-3).<br> SFRS3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SFRS3 Antibody (PB9509) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9509-srsf3-primary-antibodies-fcm-testing-3.jpgAnti-SFRS3/SRSF3 Antibody Picoband&reg; Flow Cytometry analysis of SH-SY5Y cells using anti-SFRS3 antibody (PB9509). <br> Overlay histogram showing SH-SY5Y cells stained with PB9509 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRS3 Antibody (PB9509, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat1-picoband-trade-antibody-pb9510-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9510-stat1-primary-antibodies-wb-testing-1.jpgAnti-STAT1 Antibody Picoband&reg; Western blot analysis of STAT1 using anti-STAT1 antibody (PB9510). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 antigen affinity purified polyclonal antibody (Catalog # PB9510) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT1 at approximately 83, 91 kDa. The expected band size for STAT1 is at 83, 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9510-stat1-primary-antibodies-fcm-testing-2.jpgAnti-STAT1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-STAT1 antibody (PB9510). <br> Overlay histogram showing A549 cells stained with PB9510 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT1 Antibody (PB9510, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat3-picoband-trade-antibody-pb9511-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-wb-testing-1_1.jpgAnti-STAT3 Antibody Picoband&reg; Western blot analysis of STAT3 using anti-STAT3 antibody (PB9511). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human Hacat whole cell lysates,<br> Lane 4: human U251 whole cell lysates,<br> Lane 5: human K562 whole cell lysates,<br> Lane 6: human SiHa whole cell lysates,<br> Lane 7: human RT4 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # PB9511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-wb-testing-2.jpgAnti-STAT3 Antibody Picoband&reg; Western blot analysis of STAT3 using anti-STAT3 antibody (PB9511). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat heart tissue lysates,<br> Lane 5: mouse lung tissue lysates,<br> Lane 6: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # PB9511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-fonc-11-620993-g004.jpgAnti-STAT3 Antibody Picoband&reg;The expression of leptin in serum and target protein expression in lung tissues of C57BL/6J mice. (A) Serum leptin levels in different groups of C57BL/6J mice as measured by ELISA (n =3–5 mice/group). *P < 0.05, **P < 0.01. (B) Pulmonary expression of STAT3 and GRP78 in different groups of C57BL/6J mice shown by western blotting (n=3 mice/group). *P < 0.05, **P < 0.01, ***P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.620993/full'>33708630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-fonc-11-620993-g005.jpgAnti-STAT3 Antibody Picoband&reg;Effect of leptin on lung cancer cell proliferation and apoptosis in vitro . (A) Cell viability increased in a dose-dependent manner when A549 or H460 cells were treated with increasing concentrations of leptin (0, 50, 100, and 200 ng/ml). (B) Proportion of apoptotic cells decreased when A549 was treated with 100 ng/ml leptin as measured by flow cytometry assays. Upper: representative image of flow cytometry assay. (C) Cell cycle changes were observed when A549 was treated with 100 ng/ml leptin. Upper: representative image of flow cytometry assay. (D) Western blotting for the expression of STAT3, Bcl-2, and CyclinD1 in A549 lung cancer cells with or without the inhibition of mTOR. (E) Western blotting for the expression of mTOR, STAT3, Bcl-2, and CyclinD1 in A549 lung cancer cells with or without the Inhibition of PI3K. All quantifications are made from three individual experiments. *P < 0.05, **P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.620993/full'>33708630</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-13046_2009_article_237_fig3_html.jpgAnti-STAT3 Antibody Picoband&reg;Western blot analysis of regulation of protein expression by HIF-1alpha in NCI-H446 cells . According to different treatments, all the cells were divided into four groups: control group (the cells cultured under normoxic conditions of 20% O2), Ad5-HIF-1alpha transfection group, hypoxia group (the cells cultured under normoxic conditions of 1% O2) and Ad5-siHIF-1alpha transfection group (after transfection, the cells were cultured under normoxic conditions of 1% O2). (A) Western blot analysis for IGFBP5 protein expressed by the cells of four groups. (B) Western blot analysis for SOCS1 protein expressed by the cells of four groups. (C) Densitometric analysis of the IGFBP5 and SOCS1 bands compared to the corresponding β-actin bands (*p < 0.05 expression of IGFBP5 or SOCS1 protein in Ad5-HIF-1alpha group vs. control group; ** p < 0.05 expression of IGFBP5 or SOCS1 protein in hypoxia group vs. control group; *** p < 0.05 expression of IGFBP5 or SOCS1 protein in Ad5-siHIF-1alpha group vs. control group). (D) Western blot analysis for IL-6 protein expressed by the cells of four groups. (E) Western blot analysis for STAT3 protein expressed by the cells of four groups. (F) Densitometric analysis of the IL-6 and STAT3 bands compared to the corresponding β-actin bands (*p < 0.05 expression of IL-6 or STAT3 protein in Ad5-HIF-1alpha group vs. Ad5-siHIF-1alpha group group.) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-28-150'>20003295</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-wb-testing-3_1.jpgAnti-STAT3 Antibody Picoband&reg; Western blot analysis of STAT3 using anti-STAT3 antibody (PB9511). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela- WT whole cell lysates,<br> Lane 2: human Hela-STAT3 KO whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (PB9511) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-ihc-testing-1.jpgAnti-STAT3 Antibody Picoband&reg;IHC analysis of STAT3 using anti-STAT3 antibody (PB9511). <br> STAT3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STAT3 Antibody (PB9511) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-if-testing-4.jpgAnti-STAT3 Antibody Picoband&reg; IF analysis of STAT3 using anti-STAT3 antibody (PB9511) and anti-Beta Tubulin antibody (M01857-3).<br> STAT3 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAT3 Antibody (PB9511) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-if-testing-5.jpgAnti-STAT3 Antibody Picoband&reg; IF analysis of STAT3 using anti-U2OS antibody (PB9511) and anti-Beta Tubulin antibody (M01857-3).<br> STAT3 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STAT3 Antibody (PB9511) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-fcm-testing-6.jpgAnti-STAT3 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-STAT3 antibody (PB9511). <br> Overlay histogram showing SiHa cells stained with PB9511 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT3 Antibody (PB9511, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9511-stat3-primary-antibodies-fcm-testing-7.jpgAnti-STAT3 Antibody Picoband&reg; Flow Cytometry analysis of PC-12 cells using anti-STAT3 antibody (PB9511). <br> Overlay histogram showing PC-12 cells stained with PB9511 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT3 Antibody (PB9511, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stat5b-picoband-trade-antibody-pb9512-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9512-1_1.jpgAnti-STAT5b Antibody Picoband&reg; Western blot analysis of STAT5b using anti-STAT5b antibody (PB9512).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: K562 Whole Cell Lysate,<br> Lane 2: HEK293 Whole Cell Lysate,<br> Lane 3: PANC-1 Whole Cell Lysate,<br> Lane 4: SW620 Whole Cell Lysate,<br> Lane 5: SGC-7901 Whole Cell Lysate,<br> Lane 6: Rat Lung Tissue Lysate. <br> Lane 7: Rat Liver Tissue Lysate. <br> Lane 8: Mouse Spleen Tissue Lysate. <br> Lane 9: Mouse Spleen Tissue Lysate. <br> Lane 10: Mouse Lung Tissue Lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5b antigen affinity purified polyclonal antibody (Catalog # PB9512) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT5b at approximately 90KD. The expected band size for STAT5b is at 90KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9512-2.pngAnti-STAT5b Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-STAT5b antibody (PB9512). <br> Overlay histogram showing Jurkat cells stained with PB9512 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5b Antibody (PB9512&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9512-3.pngAnti-STAT5b Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-STAT5b antibody (PB9512). <br> Overlay histogram showing A431 cells stained with PB9512 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STAT5b Antibody (PB9512&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9512-4.jpgAnti-STAT5b Antibody Picoband&reg; IF analysis of STAT5b using anti-STAT5b antibody (PB9512). <br> STAT5b was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-STAT5b Antibody (PB9512) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tmem173-picoband-trade-antibody-pb9513-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9513-sting1-primary-antibodies-wb-testing-1.jpgAnti-TMEM173 Antibody Picoband&reg;Western blot analysis of TMEM173/STING1 using anti-TMEM173/STING1 antibody (PB9513). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: mouse spleen tissue lysates,<br> Lane 4: mouse thymus tissue lysates,<br> Lane 5: mouse MH-3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM173/STING1 antigen affinity purified polyclonal antibody (PB9513) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TMEM173/STING1 at approximately 35 kDa. The expected band size for TMEM173/STING1 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9513-sting1-primary-antibodies-ihc-testing-1.jpgAnti-TMEM173 Antibody Picoband&reg;IHC analysis of TMEM173/STING1 using anti-TMEM173/STING1 antibody (PB9513). <br>TMEM173/STING1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TMEM173/STING1 Antibody (PB9513) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9513-sting1-primary-antibodies-fcm-testing-1.jpgAnti-TMEM173 Antibody Picoband&reg;Flow Cytometry analysis of HepG2 cells using anti-TMEM173/STING1 antibody (PB9513). <br> Overlay histogram showing HepG2 cells stained with PB9513 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM173/STING1 Antibody (PB9513, 1 μg/1x10⁶ cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-receptor-ii-picoband-trade-antibody-pb9514-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9514-tnfrsf1b-primary-antibodies-wb-testing-1.jpgAnti-TNF Receptor II/TNFRSF1B Antibody Picoband&reg; Western blot analysis of TNFRSF1B using anti-TNFRSF1B antibody (PB9514). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SW620 whole cell lysates,<br> Lane 2: human COLO320 whole cell lysates,<br> Lane 3: human THP-1 whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: mouse ANA-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF1B antigen affinity purified polyclonal antibody (Catalog # PB9514) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF1B at approximately 60-65 kDa. The expected band size for TNFRSF1B is at 48 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnf-receptor-ii-picoband-trade-antibody-pb9515-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9515-1_1-WB-anti-stnfsr-ii-antibody.jpgAnti-TNF Receptor II/TNFRSF1B Antibody Picoband&reg; Western blot analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Thymus Tissue Lysate at 50ug,<br> Lane 2: Mouse Thymus Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II antigen affinity purified polyclonal antibody (Catalog # PB9515) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II at approximately 75 kDa. The expected band size for TNF Receptor II is at 75 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9515-2-IHC-anti-stnfsr-ii-antibody.jpgAnti-TNF Receptor II/TNFRSF1B Antibody Picoband&reg; IHC analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). <br> TNF Receptor II was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TNF Receptor II Antibody (PB9515) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9515-3-IHC-anti-stnfsr-ii-antibody.jpgAnti-TNF Receptor II/TNFRSF1B Antibody Picoband&reg; IHC analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). <br> TNF Receptor II was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TNF Receptor II Antibody (PB9515) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-traf2-picoband-trade-antibody-pb9516-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9516-1-WB-anti-traf2-picoband-antibody.jpgAnti-TRAF2 Antibody Picoband&reg; Western blot analysis of TRAF2 using anti-TRAF2 antibody (PB9516). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Testis Tissue Lysate at 50ug,<br> Lane 3: Mouse Brain Tissue Lysate at 50ug,<br> Lane 4: Human Placenta Tissue Lysate at 50ug,<br> Lane 5: 22RV1 Whole Cell Lysate at 40ug,<br> Lane 6: SMMC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF2 antigen affinity purified polyclonal antibody (Catalog # PB9516) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF2 at approximately 53 kDa. The expected band size for TRAF2 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9516-2-IHC-anti-traf2-picoband-antibody.jpgAnti-TRAF2 Antibody Picoband&reg; IHC analysis of TRAF2 using anti-TRAF2 antibody (PB9516). <br> TRAF2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRAF2 Antibody (PB9516) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpv5-picoband-trade-antibody-pb9518-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9518-1-WB-anti-trpv5-picoband-antibody.jpgAnti-TRPV5 Antibody Picoband&reg; Western blot analysis of TRPV5 using anti-TRPV5 antibody (PB9518). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Pancreas Tissue Lysate at 50ug,<br> Lane 2: Rat Lung Tissue Lysate at 50ug,<br> Lane 3: Rat Intestine Tissue Lysate at 50ug,<br> Lane 4: SW620 Whole Cell Lysate at 40ug,<br> Lane 5: COLO320 Whole Cell Lysate at 40ug,<br> Lane 6: 293T Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV5 antigen affinity purified polyclonal antibody (Catalog # PB9518) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV5 at approximately 83 kDa. The expected band size for TRPV5 is at 83 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ulk3-picoband-trade-antibody-pb9519-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9519-1-WB-anti-ulk3-picoband-antibody.jpgAnti-ULK3 Antibody Picoband&reg; Western blot analysis of ULK3 using anti-ULK3 antibody (PB9519). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Testis Tissue Lysate at 50ug,<br> Lane 3: Mouse Brain Tissue Lysate at 50ug,<br> Lane 4: Human Placenta Tissue Lysate at 50ug,<br> Lane 5: 22RV1 Whole Cell Lysate at 40ug,<br> Lane 6: SMMC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK3 antigen affinity purified polyclonal antibody (Catalog # PB9519) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK3 at approximately 53 kDa. The expected band size for ULK3 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9519-2-IHC-anti-ulk3-picoband-antibody.jpgAnti-ULK3 Antibody Picoband&reg; IHC analysis of ULK3 using anti-ULK3 antibody (PB9519). <br> ULK3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ULK3 Antibody (PB9519) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9519-3-IHC-anti-ulk3-picoband-antibody.jpgAnti-ULK3 Antibody Picoband&reg; IHC analysis of ULK3 using anti-ULK3 antibody (PB9519). <br> ULK3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ULK3 Antibody (PB9519) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9519-4.pngAnti-ULK3 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-ULK3 antibody (PB9519). <br> Overlay histogram showing U20S cells stained with PB9519 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ULK3 Antibody (PB9519&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9519-5.pngAnti-ULK3 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-ULK3 antibody (PB9519). <br> Overlay histogram showing THP-1 cells stained with PB9519 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ULK3 Antibody (PB9519&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ku70-picoband-trade-antibody-pb9520-boster.html2026-03-24T05:05:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-wb-testing-1.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; Western blot analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human LNCAP whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: human Jurkat whole cell lysates,<br> Lane 6: human K562 whole cell lysates,<br> Lane 7: human A549 whole cell lysates,<br> Lane 8: human A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ku70/XRCC6 antigen affinity purified polyclonal antibody (Catalog # PB9520) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ku70/XRCC6 at approximately 70 kDa. The expected band size for Ku70/XRCC6 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-2.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-3.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-4.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-5.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human colon adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-6.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-7.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-8.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-9.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-10.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-11.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-12.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human pancreas ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-13.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human pancreas ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-14.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-ihc-testing-15.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IHC analysis of Ku70/XRCC6 using anti-Ku70/XRCC6 antibody (PB9520). <br> Ku70/XRCC6 was detected in a paraffin-embedded section of human testicular seminoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ku70/XRCC6 Antibody (PB9520) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-if-testing-16.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IF analysis of XRCC6 using anti-XRCC6 antibody (PB9520) and anti-Beta Tubulin antibody (M01857-3).<br> XRCC6 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-XRCC6 Antibody (PB9520) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and FITC Conjugated Goat Anti-Mouse IgG (BA1101) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-if-testing-17.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; IF analysis of XRCC6 using anti-XRCC6 antibody (PB9520). <br> XRCC6 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-XRCC6 Antibody (PB9520) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9520-xrcc6-primary-antibodies-fcm-testing-18.jpgAnti-Ku70/XRCC6 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-XRCC6 antibody (PB9520). <br> Overlay histogram showing A431 cells stained with PB9520 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XRCC6 Antibody (PB9520, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il7-picoband-trade-antibody-pb9521-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9521-1-WB-anti-il-7-antibody.jpgAnti-IL7 Antibody Picoband&reg; Western blot analysis of IL7 using anti-IL7 antibody (PB9521). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: K562 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PB9521) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 75 kDa. The expected band size for IL7 is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tff1-picoband-trade-antibody-pb9522-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9522-1-WB-anti-tff1-ps2-picoband-antibody.jpgAnti-Estrogen Inducible Protein pS2/TFF1 Antibody Picoband&reg; Western blot analysis of TFF1 using anti-TFF1 antibody (PB9522). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFF1 antigen affinity purified polyclonal antibody (Catalog # PB9522) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFF1 at approximately 12 kDa. The expected band size for TFF1 is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-liver-arginase-antibody-rp1075-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1075-arg1-primary-antibodies-wb-testing-1.jpgAnti-liver Arginase/ARG1 Antibody Picoband&reg; Western blot analysis of Arginase-1/ARG1 using anti-Arginase-1/ARG1 antibody (RP1075). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, <br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arginase-1/ARG1 antigen affinity purified polyclonal antibody (RP1075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arginase-1/ARG1 at approximately 38 kDa. The expected band size for Arginase-1/ARG1 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd59-antibody-rp1076-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1076-1-IHC-anti-cd59-antibody.jpgAnti-CD59 glycoprotein CD59 Antibody Picoband&reg; IHC analysis of CD59 using anti-CD59 antibody (RP1076). <br> CD59 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD59 Antibody (RP1076) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1076-cd59-primary-antibodies-fc-testing-2.pngAnti-CD59 glycoprotein CD59 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-CD59 antibody (RP1076).<br>Overlay histogram showing K562 cells stained with RP1076 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD59 Antibody (RP1076&#44;1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1076-cd59-primary-antibodies-wb-testing-3.jpgAnti-CD59 glycoprotein CD59 Antibody Picoband&reg; Western blot analysis of CD59 using anti-CD59 antibody (RP1076). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 antigen affinity purified polyclonal antibody (Catalog # RP1076) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD59 at approximately 19 kDa. The expected band size for CD59 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1076-cd59-primary-antibodies-if-testing-4.jpgAnti-CD59 glycoprotein CD59 Antibody Picoband&reg; IF analysis of CD59 using anti-CD59 antibody (RP1076). <br> CD59 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CD59 Antibody (RP1076) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-jnk2-antibody-rp1077-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1077-jnk2-primary-antibodies-wb-testing-1.jpgAnti-JNK2/MAPK9 Antibody Picoband&reg;Western blot analysis of JNK2/MAPK9 using anti-JNK2/MAPK9 antibody (RP1077). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SiHa whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: human Caco-2 whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JNK2/MAPK9 antigen affinity purified polyclonal antibody (RP1077) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for JNK2/MAPK9 at approximately 48-50 kDa. The expected band size for JNK2/MAPK9 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1077-jnk2-primary-antibodies-if-testing-1.jpgAnti-JNK2/MAPK9 Antibody Picoband&reg;IF analysis of JNK2/MAPK9 using anti-JNK2/MAPK9 antibody (RP1077). <br> JNK2/MAPK9 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-JNK2/MAPK9 Antibody (RP1077) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1077-jnk2-primary-antibodies-fcm-testing-1.jpgAnti-JNK2/MAPK9 Antibody Picoband&reg;Flow Cytometry analysis of HEL cells using anti-JNK2/MAPK9 antibody (RP1077). <br> Overlay histogram showing HEL cells stained with RP1077 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (RP1077, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1077-jnk2-primary-antibodies-fcm-testing-2.jpgAnti-JNK2/MAPK9 Antibody Picoband&reg;Flow Cytometry analysis of HEPA1-6 cells using anti-JNK2/MAPK9 antibody (RP1077). <br> Overlay histogram showing HEPA1-6 cells stained with RP1077 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (RP1077, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1077-jnk2-primary-antibodies-fcm-testing-3.jpgAnti-JNK2/MAPK9 Antibody Picoband&reg;Flow Cytometry analysis of RH35 cells using anti-JNK2/MAPK9 antibody (RP1077). <br> Overlay histogram showing RH35 cells stained with RP1077 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JNK2/MAPK9 Antibody (RP1077, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tsg101-antibody-rp1078-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1078-tsg101-primary-antibodies-wb-testing-1.jpgAnti-TSG101 Antibody Picoband&reg;Western blot analysis of TSG101 using anti-TSG101 antibody (RP1078). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat PC-12 whole cell lysates, <br> Lane 6: mouse brain tissue lysates, <br> Lane 7: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSG101 antigen affinity purified polyclonal antibody (RP1078) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TSG101 at approximately 44 kDa. The expected band size for TSG101 is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bag2-picoband-trade-antibody-pb9524-boster.html2026-03-25T05:22:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9524-bag2-primary-antibodies-fcm-testing-3.pngAnti-BAG2 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-BAG2 antibody (PB9524). <br>Overlay histogram showing THP-1 cells stained with PB9524 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAG2 Antibody (PB9524, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9524-bag2-primary-antibodies-wb-testing-1.jpgAnti-BAG2 Antibody Picoband&reg; Western blot analysis of BAG2 using anti-BAG2 antibody (PB9524). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human K562 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAG2 antigen affinity purified polyclonal antibody (Catalog # PB9524) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAG2 at approximately 24 kDa. The expected band size for BAG2 is at 24 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9524-bag2-primary-antibodies-if-testing-2.jpgAnti-BAG2 Antibody Picoband&reg; IF analysis of BAG2 using anti-BAG2 antibody (PB9524). <br> BAG2 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BAG2 Antibody (PB9524) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcl10-picoband-trade-antibody-pb9525-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9525-bcl10-primary-antibodies-wb-testing-1.jpgAnti-Bcl10 Antibody Picoband&reg; Western blot analysis of Bcl10 using anti-Bcl10 antibody (PB9525). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl10 antigen affinity purified polyclonal antibody (Catalog # PB9525) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl10 at approximately 28 kDa. The expected band size for Bcl10 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9525-bcl10-primary-antibodies-ihc-testing-2.jpgAnti-Bcl10 Antibody Picoband&reg; IHC analysis of Bcl10 using anti-Bcl10 antibody (PB9525). <br> Bcl10 was detected in a paraffin-embedded section of human lymph node carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bcl10 Antibody (PB9525) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bche-picoband-trade-antibody-pb9526-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9526-bche-primary-antibodies-wb-testing-1.jpgAnti-Butyrylcholinesterase/BCHE Antibody Picoband&reg; Western blot analysis of BCHE using anti-BCHE antibody (PB9526). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCHE antigen affinity purified polyclonal antibody (Catalog # PB9526) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCHE at approximately 85-90 kDa. The expected band size for BCHE is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ciap2-picoband-trade-antibody-pb9527-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9527-1-WB-anti-ciap2-picoband-antibody.jpgAnti-cIAP2/BIRC3 Antibody Picoband&reg; Western blot analysis of cIAP2 using anti-cIAP2 antibody (PB9527). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cIAP2 antigen affinity purified polyclonal antibody (Catalog # PB9527) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cIAP2 at approximately 68 kDa. The expected band size for cIAP2 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmpr1b-picoband-trade-antibody-pb9528-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9528-1-WB-anti-bmpr1b-alk-6-picoband-antibody.jpgAnti-BMPR1B Antibody Picoband&reg; Western blot analysis of BMPR1B using anti-BMPR1B antibody (PB9528). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR1B antigen affinity purified polyclonal antibody (Catalog # PB9528) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR1B at approximately 57 kDa. The expected band size for BMPR1B is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9528-2-IHC-anti-bmpr1b-alk-6-picoband-antibody.jpgAnti-BMPR1B Antibody Picoband&reg; IHC analysis of BMPR1B using anti-BMPR1B antibody (PB9528). <br> BMPR1B was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BMPR1B Antibody (PB9528) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ca1-picoband-trade-antibody-pb9529-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9529-ca1-primary-antibodies-fcm-testing-7.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-CA1 antibody (PB9529). <br>Overlay histogram showing HEL cells stained with PB9529 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CA1 Antibody (PB9529, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9529-ca1-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; Western blot analysis of CA1 using anti-CA1 antibody (PB9529). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates, <br> Lane 2: rat spleen tissue lysates, <br> Lane 3: mouse spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA1 antigen affinity purified polyclonal antibody (Catalog # PB9529) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA1 at approximately 29 kDa. The expected band size for CA1 is at 29 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9529-ca1-primary-antibodies-ihc-testing-2.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; IHC analysis of CA1 using anti-CA1 antibody (PB9529). <br> CA1 was detected in a paraffin-embedded section of human adenocarcinoma of the colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CA1 Antibody (PB9529) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9529-ca1-primary-antibodies-ihc-testing-3.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; IHC analysis of CA1 using anti-CA1 antibody (PB9529). <br> CA1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CA1 Antibody (PB9529) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9529-ca1-primary-antibodies-ihc-testing-4.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; IHC analysis of CA1 using anti-CA1 antibody (PB9529). <br> CA1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CA1 Antibody (PB9529) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9529-ca1-primary-antibodies-ihc-testing-5.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; IHC analysis of CA1 using anti-CA1 antibody (PB9529). <br> CA1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CA1 Antibody (PB9529) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9529-ca1-primary-antibodies-if-testing-6.jpgAnti-Carbonic Anhydrase I/CA1 Antibody Picoband&reg; IF analysis of CA1 using anti-CA1 antibody (PB9529). <br> CA1 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CA1 Antibody (PB9529) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc6-picoband-trade-antibody-pb9531-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9531-1-WB-anti-cdc6-picoband-antibody.jpgAnti-Cdc6 Antibody Picoband&reg; Western blot analysis of Cdc6 using anti-Cdc6 antibody (PB9531). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: JURKAT Whole Cell Lysate at 40ug,<br> Lane 3: MM231 Whole Cell Lysate at 40ug,<br> Lane 4: HT1080 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc6 antigen affinity purified polyclonal antibody (Catalog # PB9531) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc6 at approximately 63 kDa. The expected band size for Cdc6 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9531-2-IHC-anti-cdc6-picoband-antibody.jpgAnti-Cdc6 Antibody Picoband&reg; IHC analysis of Cdc6 using anti-Cdc6 antibody (PB9531). <br> Cdc6 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdc6 Antibody (PB9531) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9531-3-IHC-anti-cdc6-picoband-antibody.jpgAnti-Cdc6 Antibody Picoband&reg; IHC analysis of Cdc6 using anti-Cdc6 antibody (PB9531). <br> Cdc6 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdc6 Antibody (PB9531) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hrpt2-picoband-trade-antibody-pb9532-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9532-cdc73-primary-antibodies-wb-testing-1.jpgAnti-HRPT2/CDC73 Antibody Picoband&reg; Western blot analysis of HRPT2/CDC73 using anti-HRPT2/CDC73 antibody (PB9532). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HRPT2/CDC73 antigen affinity purified polyclonal antibody (PB9532) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HRPT2/CDC73 at approximately 61 kDa. The expected band size for HRPT2/CDC73 is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk1-picoband-trade-antibody-pb9533-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9533-1-WB-anti-cdk1-cdc2-picoband-antibody.jpgAnti-CDK1 Antibody Picoband&reg; Western blot analysis of CDK1 using anti-CDK1 antibody (PB9533). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Thymus Tissue Lysate at 50ug,<br> Lane 2: Rat Spleen Tissue Lysate at 50ug,<br> Lane 3: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug,<br> Lane 5: JURKAT Whole Cell Lysate at 40ug,<br> Lane 6: NIH3T3 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 antigen affinity purified polyclonal antibody (Catalog # PB9533) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK1 at approximately 34 kDa. The expected band size for CDK1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9533-fcimb-08-00279-g007.jpgAnti-CDK1 Antibody Picoband&reg;Western blot analysis of cell-cycle related proteins. RD cells, mock-infected (M) or infected with CVA6 (I) at an MOI of 1, were collected at 0, 12, 24, 36, and 48 h. The expression of CDK4, CDK6, cyclinD1, P53, P21, P16, cyclinE1, CDK2, cyclinB1, CDK1, and VP1 proteins was analyzed. Histone is the loading control. The results were representative of three independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2018.00279/full'>30159255</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9533-fig-7-1x.jpgAnti-CDK1 Antibody Picoband&reg;Down-regulation of gene products associated with the cell cycle pathway in 75% dieting KDY rats’ liver for 2 weeks (mean + STDEV.S; n = 3). (A) Casp3, Cdk1 and Ki67 in the liver was evaluated at mRNA level, and a high value of FPKM means a high expression; (B) Casp3, Cdk1 and Ki67 in the liver was evaluated by Western blot; C, Results of B were quantified by relative brightness. FPKM, Fragments Per Kilobase of exon model per Million mapped fragments. Control, control group, KDY rats were given free approach to food and water; 75% dieting, 75% dieting group, rats were given 75% food of control group at the same age in days and given free access to water. * P < 0.05 vs control group, Student’s t -test, two-tailed. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/15999/'>37701826</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9533-2-cdk1-primary-antibodies-wb-testing-1.pngAnti-CDK1 Antibody Picoband&reg;Western blot analysis of CDK1 using anti-CDK1 antibody (A01246). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Normal group-rat colon tissue,<br> Lane 2: Model group-colon tissue from model rats,<br> Lane 3: Low dose group-colon tissue from model rats,<br> Lane 4: Medium dose group-colon tissue from model rats,<br> Lane 5: High dose group-colon tissue from model rats,<br> Lane 6: Western medicine treated-colon tissue from model rats.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK1 antigen affinity purified monoclonal antibody (A01246) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for CDK1 at approximately 34 kDa. The expected band size for CDK1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9533-2-IHC-anti-cdk1-cdc2-picoband-antibody.jpgAnti-CDK1 Antibody Picoband&reg; IHC analysis of CDK1 using anti-CDK1 antibody (PB9533). <br> CDK1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CDK1 Antibody (PB9533) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9533-3-IHC-anti-cdk1-cdc2-picoband-antibody.jpgAnti-CDK1 Antibody Picoband&reg; IHC analysis of CDK1 using anti-CDK1 antibody (PB9533). <br> CDK1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CDK1 Antibody (PB9533) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9533-4-IHC-anti-cdk1-cdc2-picoband-antibody.jpgAnti-CDK1 Antibody Picoband&reg; IHC analysis of CDK1 using anti-CDK1 antibody (PB9533). <br> CDK1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CDK1 Antibody (PB9533) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9533-5.jpgAnti-CDK1 Antibody Picoband&reg; IF analysis of CDK1 using anti-CDK1 antibody (PB9533).<br> CDK1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CDK1 Antibody (PB9533) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9533-6.pngAnti-CDK1 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-CDK1 antibody (PB9533). <br> Overlay histogram showing U937 cells stained with PB9533 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDK1 Antibody (PB9533&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk2-picoband-trade-antibody-pb9534-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9534-12957_2025_3746_fig7_html.pngAnti-Cdk2 Antibody Picoband&reg;eIF6 triggered cancer signaling pathways linked to PI3K/AKT in the advancement of GC. a , c The expression levels of apoptosis and cell cycle markers, such as Parp, Caspase 3, Bcl-2, Cytochrome C, Cyclin A, and CDK2, were measured by western blotting. Using the ImageJ program, the Western Blotting's blot density was measured. Loading control was established using β-actin. e Through western blotting, the expression level of markers associated to PI3K/AKT was ascertained. Using the ImageJ program, the Western Blotting's blot density in HGC27 and MKN45 cells ( b , d , f ) was measured. ** p <0.01; *** p <0.001; and * p <0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12957-025-03746-w'>40170052</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9534-cdk2-primary-antibodies-wb-testing-1_1.jpgAnti-Cdk2 Antibody Picoband&reg; Western blot analysis of Cdk2 using anti-Cdk2 antibody (PB9534). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurlat whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human U2OS whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: human T47D whole cell lysates, <br> Lane 6: human CACO-2 whole cell lysates, <br> Lane 7: human 293T whole cell lysates, <br> Lane 8: human PC-3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk2 antigen affinity purified polyclonal antibody (Catalog # PB9534) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk2 at approximately 30 kDa. The expected band size for Cdk2 is at 30, 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9534-cdk2-primary-antibodies-ihc-testing-2.jpgAnti-Cdk2 Antibody Picoband&reg; IHC analysis of Cdk2 using anti-Cdk2 antibody (PB9534). <br> Cdk2 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cdk2 Antibody (PB9534) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9534-cdk2-primary-antibodies-if-testing-3.jpgAnti-Cdk2 Antibody Picoband&reg; IF analysis of Cdk2 using anti-Cdk2 antibody (PB9534) and anti-Beta Tubulin antibody (M01857-3).<br> Cdk2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-Cdk2 Antibody (PB9534) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk4-picoband-trade-antibody-pb9535-boster.html2026-03-24T05:05:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9535-cdk4-primary-antibodies-wb-testing-1.jpgAnti-Cdk4 Antibody Picoband&reg; Western blot analysis of CDK4 using anti-CDK4 antibody (PA1428-1, Left) and anti-CDK4 antibody (PB9535, Right). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: rat PC-12 whole cell lysates, <br> Lane 4: mosue NIH/3T3 whole cell lysates, <br> Lane 5: mosue RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PA1428-1) and rabbit anti-CDK4 antigen affinity purified polyclonal antibody (Catalog # PB9535)at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9535-fonc-11-648985-g001.jpgAnti-Cdk4 Antibody Picoband&reg;PD promotes the anti-tumor effects of sorafenib in PC3 cells. (A) . The effects of PD, sorafenib and PD plus sorafenib on cell viability. (B, C) . The changes in the cell membrane and nucleus after treatment with PD alone, sorafenib (Sor) alone or PD plus sorafenib. (D) . The induction of apoptosis by PD, Sor and PD + Sor. (E) . The protein expression levels of Caspase 3, C-Caspase 3, PARP and C-PARP were examined after cells were treated with 10 μM PD alone, 10 μM sorafenib alone or PD plus sorafenib for 6 h. (F) . The ability of cells to achieve colony growth was assessed after treatment with PD alone, sorafenib alone or PD plus sorafenib for 10 days. (G) . The proliferation of cells was monitored using the CFDA SE assay after treatment with PD alone, sorafenib alone or PD plus sorafenib for 5 days. (H, I) . The cell cycle distribution of PC3 cells following treatment with PD alone, sorafenib alone or PD plus sorafenib for 24 h after pre-treatment with (H) or without (I) 2 mM thymidine. (J) . Changes in the protein expression levels of CDK4, CDK6 and cyclin D1 after treatment with 5 μM PD alone, 2.5 μM sorafenib alone or PD plus sorafenib for 24 h. * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fonc.2021.648985/full'>34026624</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9535-2-IHC-anti-cdk4-picoband-antibody.jpgAnti-Cdk4 Antibody Picoband&reg; IHC analysis of Cdk4 using anti-Cdk4 antibody (PB9535). <br> Cdk4 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdk4 Antibody (PB9535) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9535-3-IHC-anti-cdk4-picoband-antibody.jpgAnti-Cdk4 Antibody Picoband&reg; IHC analysis of Cdk4 using anti-Cdk4 antibody (PB9535). <br> Cdk4 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdk4 Antibody (PB9535) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9535-4-IHC-anti-cdk4-picoband-antibody.jpgAnti-Cdk4 Antibody Picoband&reg; IHC analysis of Cdk4 using anti-Cdk4 antibody (PB9535). <br> Cdk4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdk4 Antibody (PB9535) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9535-cdk4-primary-antibodies-if-testing-5.jpgAnti-Cdk4 Antibody Picoband&reg; IF analysis of CDK4 using anti-CDK4 antibody (PB9535). <br> CDK4 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CDK4 Antibody (PB9535) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk7-picoband-trade-antibody-pb9536-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9536-1_1-WB-anti-cdk7-picoband-antibody.jpgAnti-Cdk7 Antibody Picoband&reg; Western blot analysis of Cdk7 using anti-Cdk7 antibody (PB9536). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug,<br> Lane 4: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 5: 293T Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk7 antigen affinity purified polyclonal antibody (Catalog # PB9536) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk7 at approximately 39 kDa. The expected band size for Cdk7 is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk9-picoband-trade-antibody-pb9537-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9537-1-WB-anti-cdk9-picoband-antibody.jpgAnti-Cdk9 Antibody Picoband&reg; Western blot analysis of Cdk9 using anti-Cdk9 antibody (PB9537). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: JURKAT Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk9 antigen affinity purified polyclonal antibody (Catalog # PB9537) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdk9 at approximately 43 kDa. The expected band size for Cdk9 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9537-2-IHC-anti-cdk9-picoband-antibody.jpgAnti-Cdk9 Antibody Picoband&reg; IHC analysis of Cdk9 using anti-Cdk9 antibody (PB9537). <br> Cdk9 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdk9 Antibody (PB9537) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9537-3-IHC-anti-cdk9-picoband-antibody.jpgAnti-Cdk9 Antibody Picoband&reg; IHC analysis of Cdk9 using anti-Cdk9 antibody (PB9537). <br> Cdk9 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cdk9 Antibody (PB9537) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cenpa-picoband-trade-antibody-pb9538-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9538-1-WB-anti-cenpa-picoband-antibody.jpgAnti-CENPA Antibody Picoband&reg; Western blot analysis of CENPA using anti-CENPA antibody (PB9538). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Rat Lung Tissue Lysate at 50ug,<br> Lane 3: Rat Pancreas Tissue Lysate at 50ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CENPA antigen affinity purified polyclonal antibody (Catalog # PB9538) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CENPA at approximately 16 kDa. The expected band size for CENPA is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-comt-picoband-trade-antibody-pb9539-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9539-comt-primary-antibodies-wb-testing-1_1.jpgAnti-COMT Antibody Picoband&reg;Western blot analysis of COMT using anti-COMT antibody (PB9539). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat liver tissue lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COMT antigen affinity purified polyclonal antibody (Catalog # PB9539) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COMT at approximately 26 kDa. The expected band size for COMT is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9539-comt-primary-antibodies-ihc-testing-1.jpgAnti-COMT Antibody Picoband&reg;IHC analysis of COMT using anti-COMT antibody (PB9539). <br> COMT was detected in a paraffin-embedded section of human human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COMT Antibody (PB9539) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9539-comt-primary-antibodies-fcm-testing-1.jpgAnti-COMT Antibody Picoband&reg;Flow Cytometry analysis of U251 cells using anti-COMT antibody (PB9539). <br> Overlay histogram showing U251 cells stained with PB9539 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-COMT Antibody (PB9539, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cryptochrome-i-picoband-trade-antibody-pb9540-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9540-1-WB-anti-cryptochrome-i-picoband-antibody.jpgAnti-Cryptochrome I/CRY1 Antibody Picoband&reg; Western blot analysis of Cryptochrome I using anti-Cryptochrome I antibody (PB9540). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate Lane 2: Rat Testis Tissue Lysate Lane 3: HELA Whole Cell Lysate After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cryptochrome I antigen affinity purified polyclonal antibody (Catalog # PB9540) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cryptochrome I at approximately 66KD. The expected band size for Cryptochrome I is at 66KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9540-cry1-primary-antibodies-ihc-testing-5.jpgAnti-Cryptochrome I/CRY1 Antibody Picoband&reg;IHC analysis of Cryptochrome 1/CRY1 using anti-Cryptochrome 1/CRY1 antibody (PB9540). <br>Cryptochrome 1/CRY1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cryptochrome 1/CRY1 Antibody (PB9540) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9540-2.jpgAnti-Cryptochrome I/CRY1 Antibody Picoband&reg; IHC analysis of CRY1 using anti-CRY1 antibody (PB9540). CRY1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRY1 Antibody (PB9540) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9540-3.jpgAnti-Cryptochrome I/CRY1 Antibody Picoband&reg; IHC analysis of CRY1 using anti-CRY1 antibody (PB9540). CRY1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRY1 Antibody (PB9540) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9540-4.jpgAnti-Cryptochrome I/CRY1 Antibody Picoband&reg; IHC analysis of CRY1 using anti-CRY1 antibody (PB9540). CRY1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRY1 Antibody (PB9540) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ctgf-picoband-trade-antibody-pb9541-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9541-1-WB-anti-ctgf-ccn2-picoband-antibody.jpgAnti-CTGF Antibody Picoband&reg; Western blot analysis of CTGF using anti-CTGF antibody (PB9541). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Rat Thymus Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTGF antigen affinity purified polyclonal antibody (Catalog # PB9541) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTGF at approximately 38 kDa. The expected band size for CTGF is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9541-2-IHC-anti-ctgf-ccn2-picoband-antibody.jpgAnti-CTGF Antibody Picoband&reg; IHC analysis of CTGF using anti-CTGF antibody (PB9541). <br> CTGF was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CTGF Antibody (PB9541) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9541-fphar-13-808576-g005.jpgAnti-CTGF Antibody Picoband&reg;The key fibrosis-related gene and signaling pathway that are inhibited by KD (A) The results of high-throughput sequencing of the transcriptome showed that the expression of CTGF gene increased after irradiation (B) The results of high-throughput sequencing of the transcriptome showed that the expression of CTGF gene decreased after the administration of KD (C) The results of Real-Time PCR were consistent with the results of the sequencing. The expression of CTGF mRNA increased in the IR group, but decreased in the IR + KD group (D) The results of western blotting were consistent with the results of sequencing. The expression of CTGF protein increased in the IR group, but decreased in the IR + KD group (E) In the transcriptome KEGG gene pathway enrichment map, the TGF-β pathway was closely related to CTGF and RILF (F) The results of tissue TGF-β1 immunohistochemistry indicated that in the IR + KD group the expression of TGF-β1 in the liver tissue decreased compared with the IR group. For all results in this figure, original magnification, ×40 and ×100. Mean ± SEM. * p < 0.05, ** p < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8814438/&orig_host=www.ncbi.nlm.nih.gov'>35126163</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9541-fphar-13-808576-g006.jpgAnti-CTGF Antibody Picoband&reg;KD ameliorates the RILF by inhibiting TGF-β1/Smad/CTGF pathway (A) Exogenous TGF-β1 led to the increased expression of CTGF protein (B) Exogenous TGF-β1 induced an increase in the expression of collagen and a -SMA proteins (C) The TGF-β1 activity in the conditioned media significantly increased after 6Gy irradiation compared with the control group, while the TGF-β1 activity in the conditioned media in the IR + KD group decreased compared with the IR group (D-G) The expressions of TGF-β1 and downstream Smad2/3 as well as phosphorylated Smad2/3 reduced in the IR + KD group compared with the IR group. Mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8814438/&orig_host=www.ncbi.nlm.nih.gov'>35126163</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9541-13098_2013_article_300_fig3_html.jpgAnti-CTGF Antibody Picoband&reg;Top panel: representative slides showing immunohistochemical staining of connective tissue growth factor (CTGF) (stained in brown as shown by arrow) in the myocardium. Slides A , B , C , D represent control, diabetic, diabetic treated with low dose (2 μg · kg −1 .d −1 ) of exenatide and diabetic treated with high dose (10 μg · kg −1 .d −1 ) of exenatide, respectively. Magnifications × 400. Bottom: bar graph shows quantitative analysis of myocardial CTGF expression in control, diabetic and diabetic treated with low (2 μg · kg −1 .d −1 ) or high (10 μg · kg −1 .d −1 ) dose of exenatide. The CTGF expression was quantified using the average value of gray scale which had an inverse proportion to the positive stained intensity. Control (C, n = 7), diabetic (D, n = 10), diabetic treated with low dose (2 μg · kg −1 .d −1 ) of exenatide (DTL, n = 10), diabetic treated with high dose (10 μg · kg −1 .d −1 ) of exenatide (DTH, n = 9). Data are expressed as mean ± S.E.M. *P < 0.05 different from control, # P < 0.05 different from diabetic, $ P < 0.05 different from diabetic treated with low dose (2 μg · kg −1 .d −1 ) of exenatide. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1758-5996-6-29'>24576329</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cullin-1-picoband-trade-antibody-pb9542-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9542-cul1-primary-antibodies-wb-testing-1.jpgAnti-Cullin 1/CUL1 Antibody Picoband&reg; Western blot analysis of Cullin 1/CUL1 using anti-Cullin 1/CUL1 antibody (PB9542). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human SiHa whole cell lysates, <br> Lane 3: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 1/CUL1 antigen affinity purified polyclonal antibody (PB9542) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cullin 1/CUL1 at approximately 90 kDa. The expected band size for Cullin 1/CUL1 is at 90 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyld-picoband-trade-antibody-pb9543-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9543-1-WB-anti-cyld-cylindromatosis-1-picoband-antibody.jpgAnti-CYLD Antibody Picoband&reg; Western blot analysis of CYLD using anti-CYLD antibody (PB9543). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Testis Tissue Lysate at 50ug,<br> Lane 2: Rat Brain Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYLD antigen affinity purified polyclonal antibody (Catalog # PB9543) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYLD at approximately 107 kDa. The expected band size for CYLD is at 107 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp1a1-picoband-trade-antibody-pb9544-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9544-2-IHC-anti-cyp1a1-cytochrome-p450-1a1-picoband-antibody.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; IHC analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544).<br> CYP1A1 was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1A1 Antibody (PB9544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9544-cyp1a1-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg;Western blot analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat RH-35 whole cell lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse liver tissue lysates,<br> Lane 6: mouse HEPA1/6 whole cell lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A1 antigen affinity purified polyclonal antibody (Catalog # PB9544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP1A1 at approximately 58 kDa. The expected band size for CYP1A1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9544-3-IHC-anti-cyp1a1-cytochrome-p450-1a1-picoband-antibody.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; IHC analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544).<br> CYP1A1 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1A1 Antibody (PB9544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9544-4-IHC-anti-cyp1a1-cytochrome-p450-1a1-picoband-antibody.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; IHC analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544).<br> CYP1A1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1A1 Antibody (PB9544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9544-5-IHC-anti-cyp1a1-cytochrome-p450-1a1-picoband-antibody.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; IHC analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544).<br> CYP1A1 was detected in frozen section of Mouse Kidney Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1A1 Antibody (PB9544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9544-6-IHC-anti-cyp1a1-cytochrome-p450-1a1-picoband-antibody.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; IHC analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544).<br> CYP1A1 was detected in frozen section of Human Placenta Tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1A1 Antibody (PB9544) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9544-cyp1a1-primary-antibodies-fc-testing-7.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-CYP1A1 antibody (PB9544).<br>Overlay histogram showing CACO-2 cells stained with PB9544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (PB9544&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9544-cyp1a1-primary-antibodies-fc-testing-8.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-CYP1A1 antibody (PB9544).<br>Overlay histogram showing Hela cells stained with PB9544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (PB9544&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9544-cyp1a1-primary-antibodies-if-testing-10.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; IF analysis of CYP1A1 using anti-CYP1A1 antibody (PB9544). <br> CYP1A1 was detected in immunocytochemical section of Caco-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CYP1A1 Antibody (PB9544) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9544-cyp1a1-primary-antibodies-fc-testing-9.jpgAnti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-CYP1A1 antibody (PB9544).<br>Overlay histogram showing K562 cells stained with PB9544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (PB9544&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp1a2-picoband-trade-antibody-pb9545-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9545-cyp1a2-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome P450 1A2/CYP1A2 Antibody Picoband&reg; Western blot analysis of CYP1A2 using anti-CYP1A2 antibody (PB9545). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HCCP tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1A2 antigen affinity purified polyclonal antibody (Catalog # PB9545) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP1A2 at approximately 58 kDa. The expected band size for CYP1A2 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9545-cyp1a2-primary-antibodies-ihc-testing-5.jpgAnti-Cytochrome P450 1A2/CYP1A2 Antibody Picoband&reg;IHC analysis of CYP1A2 using anti-CYP1A2 antibody (PB9545). <br>CYP1A2 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYP1A2 Antibody (PB9545) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9545-2-IHC-anti-cyp1a2-cytochrome-p450-1a2-picoband-antibody.jpgAnti-Cytochrome P450 1A2/CYP1A2 Antibody Picoband&reg; IHC analysis of CYP1A2 using anti-CYP1A2 antibody (PB9545). <br> CYP1A2 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP1A2 Antibody (PB9545) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9545-3-IHC-anti-cyp1a2-cytochrome-p450-1a2-picoband-antibody.jpgAnti-Cytochrome P450 1A2/CYP1A2 Antibody Picoband&reg; IHC analysis of CYP1A2 using anti-CYP1A2 antibody (PB9545). <br> CYP1A2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP1A2 Antibody (PB9545) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9545-4-IHC-anti-cyp1a2-cytochrome-p450-1a2-picoband-antibody.jpgAnti-Cytochrome P450 1A2/CYP1A2 Antibody Picoband&reg; IHC analysis of CYP1A2 using anti-CYP1A2 antibody (PB9545). <br> CYP1A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP1A2 Antibody (PB9545) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp1b1-picoband-trade-antibody-pb9546-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9546-cyp1b1-primary-antibodies-wb-testing-1.jpgAnti-CYP1B1 Antibody Picoband&reg; Western blot analysis of CYP1B1 using anti-CYP1B1 antibody (PB9546). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: rat heart tissue lysates, <br> Lane 4: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP1B1 antigen affinity purified polyclonal antibody (Catalog # PB9546) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP1B1 at approximately 61 kDa. The expected band size for CYP1B1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9546-41467_2023_38406_fig3_html.pngAnti-CYP1B1 Antibody Picoband&reg;aHSC release LTB4R2 ligands in a manner dependent on CYP1B1. a TEAD-luciferase activity in Huh7 cells treated with conditioned medium (CM) from LX2 cells transduced with scrambled shRNA (SCRsh-CM) vs. SCD-shRNA (SCDsh-CM) as compared to the media without Huh7 cells (Media). * p < 0.05 by two-sided t-test. Data presented as means ± SEM ( n = 4 separate experiments). b TEAD-luciferase activity in Huh7 cells exposed to SCRsh-CM vs. SCDsh-CM in the presence of the TBXAS1 inhibitor Ozagarel or vehicle DMSO. * p < 0.001 vs. Media, # p < 0.001 vs. DMSO by two-sided t-test. Data presented as means ± SEM ( n = 3 experiments). c A scRNA - seq t-SNE plot showing Cyp1b1 + cells and violin plots revealing selective Cyp1b1 expression by Fbln2 + cells. Cell numbers for different cell type groups are provided in Supplementary Data . d Contour FACS plots of liver mesenchymal cells isolated from control vs. DEN + WAD treated Rosa26mTmG;Col1a1-Cre; ( mTmG;CC ) mouse, gated by DAPI ( Y -axis) for Vit A fluorescence and FITC (X-axis) for Col1a1-GFP, revealing VitA + GFP - quiescent HSC (blue), VitA + GFP + aHSC (red), and VitA − GFP + cells (green). FACS gating strategies are provided in . e scRNA-seq analysis for expression of 12-HHTrE biosynthetic genes in VitA + GFP + (top) and VitA − GFP + (bottom) subpopulations from DEN+WAD mouse (DEN) vs. normal (Cont.) livers. f Violin plots for Cyp1b1 expression by subpopulations based on Lrat , Thy1 , and Fbln2 expression in VitA + GFP + cells from the DEN mouse and g in VitA − GFP + cells. (See Supplementary Data for parameter values for violin plots and cell numbers for different subpopulations). h CRISPR/Cas9 ablation of CYP1B1 in LX2 cells using the guide RNA-A (sgRNA-A) or -B (sgRNA-B) (top), represses CYP1B1 mRNA. * p < 0.05 vs. control by two-sided t-test. Data presented as means ± SEM ( n = 3 separate samples). i Oxylipin concentrations in CM from LX2 cells with CYP1B1 KD are described above. * p < 0.05 vs. control by two-sided t-test. Data presented as means ± SEM ( n = 4 separate samples). (Raw data provided in Supplementary Data in Supplementary File). j Reduced stimulatory effects of CM from CYP1B1 ablated LX2 cells on the TEAD promoter activity in Huh7 cells (right). *** p < 0.001 vs. Control CM by two-sided t-test. Data presented as means ± SEM ( n = 3 experiments). For all relevant figures, source data and exact p values are provided in the file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-023-38406-8'>37156770</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9546-41467_2023_38406_fig4_html.pngAnti-CYP1B1 Antibody Picoband&reg;HCC growth is dependent on LTB4R2. a IB analysis of LTB4R2 and NaKATPase (membrane), pYAP1, pGSK3β, GSK3β, HuR, GAPDH (cytosolic), YAP1 and CTNNB1 (nuclear) proteins from B6 mice subjected to the DEN + WAD regimen and injected with AAV vector (4 × 10 11 GC per mouse) expressing SCR-shRNA (SCR-sh) vs. LTB4R2-shRNA (LTB4R2-sh) one month prior to the end of experiment ( n = 6 mice per group). b Liver tumor development in the mice with SCR-sh vs. LTB4R2-sh treatment depicted by representative images and total tumor volume and multiplicity. * p < 0.05 vs. SCR-sh mice by two-sided t-test. Data presented as means ± SEM ( n = 9 mice each). c qPCR data for oxylipin synthetic genes, LTB4R2 , and YAP1 in patient HCC ( n = 6) vs. normal subject livers ( n = 6). * p < 0.05, ** p < 0.01 vs. normal liver by two-sided t-test. Data presented as means ± SEM. d Representative IHC-HRP staining for CYP1B1 and LTB4R2 of patient HCC liver sections (×200) from four patient samples examined. Areas demarked by broken lines are HCC. e Top: Growth of patient HCC organoid (model-1 and model-2) in the presence of DMSO (vehicle) or the LTB4R2 antagonist LY255283. * p < 0.05, ** p < 0.01, *** p < 0.005 vs. DMSO by two-way ANOVA with post hoc test. Data presented as means ± SEM ( n = 3 experiments). Bottom: Growth of patient HCC organoid model-1 and model-2 without (CTRL) or with infection with adenovirus expressing scrambled shRNA ( shCTRL ) or LTB4R2 shRNA (shLTB4R2). * p < 0.05, *** p < 0.005 vs. DMSO ( n = 3 experiments). f Schematic diagram of HCC promotion initiated by SCD-CYP1B1-dependent release of 12-HHTrE by Lart + Fbln2 + aHSC, activating LTB4R2-CTNNB1-YAP1 pathway in HCC cell. For all relevant figures , source data and exact p values are provided in the file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-023-38406-8'>37156770</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9546-2-IHC-anti-cyp1b1-picoband-antibody.jpgAnti-CYP1B1 Antibody Picoband&reg; IHC analysis of CYP1B1 using anti-CYP1B1 antibody (PB9546). <br> CYP1B1 was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1B1 Antibody (PB9546) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9546-3-IHC-anti-cyp1b1-picoband-antibody.jpgAnti-CYP1B1 Antibody Picoband&reg; IHC analysis of CYP1B1 using anti-CYP1B1 antibody (PB9546). <br> CYP1B1 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1B1 Antibody (PB9546) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9546-4-IHC-anti-cyp1b1-picoband-antibody.jpgAnti-CYP1B1 Antibody Picoband&reg; IHC analysis of CYP1B1 using anti-CYP1B1 antibody (PB9546). <br> CYP1B1 was detected in paraffin-embedded section of Human Liver Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CYP1B1 Antibody (PB9546) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9546-cyp1b1-primary-antibodies-fc-testing-5.jpgAnti-CYP1B1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-CYP1B1 antibody (PB9546). <br>Overlay histogram showing SiHa cells stained with PB9546 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1B1 Antibody (PB9546&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp24a1-picoband-trade-antibody-pb9547-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9547-cyp24a1-primary-antibodies-wb-testing-1.jpgAnti-CYP24A1 Antibody Picoband&reg;Western blot analysis of CYP24A1 using anti-CYP24A1 antibody (PB9547). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP24A1 antigen affinity purified polyclonal antibody (PB9547) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CYP24A1 at approximately 59 kDa. The expected band size for CYP24A1 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9547-2-IHC-anti-cyp24a1-cyp24-picoband-antibody.jpgAnti-CYP24A1 Antibody Picoband&reg; IHC analysis of CYP24A1 using anti-CYP24A1 antibody (PB9547). <br> CYP24A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP24A1 Antibody (PB9547) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9547-41598_2024_75338_fig5_html.pngAnti-CYP24A1 Antibody Picoband&reg;SARS-CoV-2 infection induces abnormal vitamin D metabolism. ( A ) Representative immunofluorescence images of VDR (red: VDR, green: LTL, and blue: nuclei) in mouse kidneys seven days after SARS-CoV-2 or vehicle treatment and ( B ) quantification of positive nuclei per field. Each data from male and female mice were shown with closed and opened symbols. ( C ) VDR mRNA levels seven days after infection were quantified using qPCR (means ± SD, n = 6/group). ( D , F ) Representative immunohistochemistry images of CYP27B1 ( D ), and CYP24A1 ( F ) in mouse kidneys seven days after SARS-CoV-2. mRNA levels of CYP27B1 ( E ) and CYP24A1 ( G ) seven days after infection were quantified using qPCR (means ± SD, n = 6/group). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41598-024-75338-9'>39414885</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9547-3-IHC-anti-cyp24a1-cyp24-picoband-antibody.jpgAnti-CYP24A1 Antibody Picoband&reg; IHC analysis of CYP24A1 using anti-CYP24A1 antibody (PB9547). <br> CYP24A1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP24A1 Antibody (PB9547) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9547-4-IHC-anti-cyp24a1-cyp24-picoband-antibody.jpgAnti-CYP24A1 Antibody Picoband&reg; IHC analysis of CYP24A1 using anti-CYP24A1 antibody (PB9547). <br> CYP24A1 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP24A1 Antibody (PB9547) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp27b1-picoband-trade-antibody-pb9548-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9548-cyp27b1-primary-antibodies-wb-testing-1.jpgAnti-CYP27B1 Antibody Picoband&reg; Western blot analysis of CYP27B1 using anti-CYP27B1 antibody (PB9548). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Caco-2 whole cell lysates,<br> Lane 3: rat heart tissue lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse heart tissue lysates,<br> Lane 6: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP27B1 antigen affinity purified polyclonal antibody (Catalog # PB9548) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP27B1 at approximately 57 kDa. The expected band size for CYP27B1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9548-2-IHC-anti-cyp27b1-picoband-antibody.jpgAnti-CYP27B1 Antibody Picoband&reg; IHC analysis of CYP27B1 using anti-CYP27B1 antibody (PB9548). <br> CYP27B1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP27B1 Antibody (PB9548) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9548-3-IHC-anti-cyp27b1-picoband-antibody.jpgAnti-CYP27B1 Antibody Picoband&reg; IHC analysis of CYP27B1 using anti-CYP27B1 antibody (PB9548). <br> CYP27B1 was detected in a paraffin-embedded section of human kidney cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP27B1 Antibody (PB9548) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccn1-picoband-trade-antibody-pb9549-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9549-ccn1-primary-antibodies-wb-testing-1.jpgAnti-CCN1/CYR61 Antibody Picoband&reg;Western blot analysis of CCN1/CYR61 using anti-CCN1/CYR61 antibody (PB9549). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SiHa whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: human U2OS whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: rat kidney tissue lysates,<br> Lane 7: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCN1/CYR61 antigen affinity purified polyclonal antibody (PB9549) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CCN1/CYR61 at approximately 42 kDa. The expected band size for CCN1/CYR61 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9549-ccn1-primary-antibodies-ihc-testing-1.jpgAnti-CCN1/CYR61 Antibody Picoband&reg;IHC analysis of CCN1/CYR61 using anti-CCN1/CYR61 antibody (PB9549). <br> CCN1/CYR61 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCN1/CYR61 Antibody (PB9549) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-daxx-picoband-trade-antibody-pb9550-boster.html2026-04-01T05:01:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9550-daxx-primary-antibodies-wb-testing-1.jpgAnti-Daxx Antibody Picoband&reg; Western blot analysis of Daxx using anti-Daxx antibody (PB9550). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: rat PC-12 whole cell lysates, <br> Lane 3: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Daxx antigen affinity purified polyclonal antibody (Catalog # PB9550) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Daxx at approximately 115 kDa. The expected band size for Daxx is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9550-2-IHC-anti-daxx-picoband-antibody.jpgAnti-Daxx Antibody Picoband&reg; IHC analysis of DAXX using anti-DAXX antibody (PB9550). <br> DAXX was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DAXX Antibody (PB9550) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9550-3-IHC-anti-daxx-picoband-antibody.jpgAnti-Daxx Antibody Picoband&reg; IHC analysis of DAXX using anti-DAXX antibody (PB9550). <br> DAXX was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DAXX Antibody (PB9550) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9550-4_1-IF-anti-daxx-picoband-antibody.jpgAnti-Daxx Antibody Picoband&reg; IHC analysis of DAXX using anti-DAXX antibody (PB9550).<br> DAXX was detected in immunocytochemical section of SMMC-7721 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-DAXX Antibody (PB9550) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9550-5_1-IF-anti-daxx-picoband-antibody.jpgAnti-Daxx Antibody Picoband&reg; IHC analysis of DAXX using anti-DAXX antibody (PB9550).<br> DAXX was detected in immunocytochemical section of A549 Cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-DAXX Antibody (PB9550) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9550-daxx-primary-antibodies-if-testing-6.jpgAnti-Daxx Antibody Picoband&reg; IF analysis of Daxx using anti-Daxx antibody (PB9550). <br> Daxx was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Daxx Antibody (PB9550) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9550-daxx-primary-antibodies-if-testing-7.jpgAnti-Daxx Antibody Picoband&reg; IF analysis of Daxx using anti-Daxx antibody (PB9550) and anti-Tubulin Alpha antibody (M03989-3).<br>Daxx was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Daxx Antibody (PB9550) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9550-daxx-primary-antibodies-fcm-testing-8.jpgAnti-Daxx Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-Daxx antibody (PB9550).<br>Overlay histogram showing 293T cells stained with PB9550 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Daxx Antibody (PB9550,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dkk2-picoband-trade-antibody-pb9551-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9551-1-WB-anti-dkk2-picoband-antibody.jpgAnti-DKK2 Antibody Picoband&reg; Western blot analysis of DKK2 using anti-DKK2 antibody (PB9551). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 2: Rat Brain Tissue Lysate at 50ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK2 antigen affinity purified polyclonal antibody (Catalog # PB9551) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK2 at approximately 28 kDa. The expected band size for DKK2 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sap97-picoband-trade-antibody-pb9552-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9552-1-WB-anti-sap97-picoband-antibody.jpgAnti-SAP97/DLG1 Antibody Picoband&reg; Western blot analysis of SAP97 using anti-SAP97 antibody (PB9552). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Lung Tissue Lysate at 50ug,<br> Lane 2: Mouse Lung Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: MM231 Whole Cell Lysate at 40ug,<br> Lane 5: COLO320 Whole Cell Lysate at 40ug,<br> Lane 6: A549 Whole Cell Lysate at 40ug,<br> Lane 7: NIH3T3 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP97 antigen affinity purified polyclonal antibody (Catalog # PB9552) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP97 at approximately 130 kDa. The expected band size for SAP97 is at 130 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9552-2-IHC-anti-sap97-picoband-antibody.jpgAnti-SAP97/DLG1 Antibody Picoband&reg; IHC analysis of SAP97 using anti-SAP97 antibody (PB9552). <br> SAP97 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SAP97 Antibody (PB9552) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ebag9-picoband-trade-antibody-pb9553-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9553-1-WB-anti-ebag9-rcas1-picoband-antibody.jpgAnti-EBAG9 Antibody Picoband&reg; Western blot analysis of EBAG9 using anti-EBAG9 antibody (PB9553). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Testis Tissue Lysate at 50ug,<br> Lane 2: 22RV1 Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 5: JURKAT Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EBAG9 antigen affinity purified polyclonal antibody (Catalog # PB9553) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EBAG9 at approximately 34 kDa. The expected band size for EBAG9 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9553-2-IHC-anti-ebag9-rcas1-picoband-antibody.jpgAnti-EBAG9 Antibody Picoband&reg; IHC analysis of EBAG9 using anti-EBAG9 antibody (PB9553). <br> EBAG9 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-EBAG9 Antibody (PB9553) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9553-3-IHC-anti-ebag9-rcas1-picoband-antibody.jpgAnti-EBAG9 Antibody Picoband&reg; IHC analysis of EBAG9 using anti-EBAG9 antibody (PB9553). <br> EBAG9 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-EBAG9 Antibody (PB9553) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9553-4-IHC-anti-ebag9-rcas1-picoband-antibody.jpgAnti-EBAG9 Antibody Picoband&reg; IHC analysis of EBAG9 using anti-EBAG9 antibody (PB9553). <br> EBAG9 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-EBAG9 Antibody (PB9553) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ednrb-picoband-trade-antibody-pb9554-boster.html2026-03-24T05:05:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9554-1-WB-anti-ednrb-endothelin-b-receptor-picoband-antibody.jpgAnti-Endothelin B Receptor/EDNRB Antibody Picoband&reg; Western blot analysis of EDNRB using anti-EDNRB antibody (PB9554). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EDNRB antigen affinity purified polyclonal antibody (Catalog # PB9554) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EDNRB at approximately 50 kDa. The expected band size for EDNRB is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9554-2-IHC-anti-ednrb-endothelin-b-receptor-picoband-antibody.jpgAnti-Endothelin B Receptor/EDNRB Antibody Picoband&reg; IHC analysis of EDNRB using anti-EDNRB antibody (PB9554). <br> EDNRB was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-EDNRB Antibody (PB9554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9554-3-IHC-anti-ednrb-endothelin-b-receptor-picoband-antibody.jpgAnti-Endothelin B Receptor/EDNRB Antibody Picoband&reg; IHC analysis of EDNRB using anti-EDNRB antibody (PB9554). <br> EDNRB was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-EDNRB Antibody (PB9554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9554-4-IHC-anti-ednrb-endothelin-b-receptor-picoband-antibody.jpgAnti-Endothelin B Receptor/EDNRB Antibody Picoband&reg; IHC analysis of EDNRB using anti-EDNRB antibody (PB9554). <br> EDNRB was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-EDNRB Antibody (PB9554) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9554-5.jpgAnti-Endothelin B Receptor/EDNRB Antibody Picoband&reg; IF analysis of EDNRB using anti-EDNRB antibody (PB9554). <br> EDNRB was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EDNRB Antibody (PB9554) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9554-6.jpgAnti-Endothelin B Receptor/EDNRB Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-EDNRB antibody (PB9554).<br>Overlay histogram showing A431 cells stained with PB9554 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EDNRB Antibody (PB9554,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-parvin-alpha-picoband-trade-antibody-pb9555-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9555-1-WB-anti-parvin-alpha-picoband-antibody.jpgAnti-Parvin alpha/PARVA Antibody Picoband&reg; Western blot analysis of Parvin alpha using anti-Parvin alpha antibody (PB9555). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: SW620 Whole Cell Lysate at 40ug,<br> Lane 4: HEPA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Parvin alpha antigen affinity purified polyclonal antibody (Catalog # PB9555) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Parvin alpha at approximately 42 kDa. The expected band size for Parvin alpha is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9555-2-IHC-anti-parvin-alpha-picoband-antibody.jpgAnti-Parvin alpha/PARVA Antibody Picoband&reg; IHC analysis of Parvin alpha using anti-Parvin alpha antibody (PB9555). <br> Parvin alpha was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Parvin alpha Antibody (PB9555) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkc-eta-picoband-trade-antibody-pb9556-boster.html2026-04-01T05:01:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9556-1-WB-anti-pkc-eta-picoband-antibody.jpgAnti-PKC eta/PRKCH Antibody Picoband&reg; Western blot analysis of PKC eta using anti-PKC eta antibody (PB9556). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: A431 Whole Cell Lysate<br> Lane 2: A549 Whole Cell Lysate<br> Lane 3: HELA Whole Cell Lysate<br> Lane 4: SKOV Whole Cell Lysate <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC eta antigen affinity purified polyclonal antibody (Catalog # PB9556) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC eta at approximately 78KD. The expected band size for PKC eta is at 78KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9556-pkc-eta-primary-antibodies-fc-testing-2.jpgAnti-PKC eta/PRKCH Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-PKC-eta antibody (PB9556). <br>Overlay histogram showing A431 cells stained with PB9556 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9556-pkc-eta-primary-antibodies-fc-testing-3.jpgAnti-PKC eta/PRKCH Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-PKC-eta antibody (PB9556). <br>Overlay histogram showing K562 cells stained with PB9556 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9556-pkc-eta-primary-antibodies-fc-testing-4.jpgAnti-PKC eta/PRKCH Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-PKC-eta antibody (PB9556). <br>Overlay histogram showing MCF-7 cells stained with PB9556 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKC-eta Antibody (PB9556&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sp2-picoband-trade-antibody-pb9557-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9557-1-WB-anti-sp2-picoband-antibody.jpgAnti-SP2 transcription factor Antibody Picoband&reg; Western blot analysis of SP2 using anti-SP2 antibody (PB9557). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Lung Tissue Lysate at 50ug,<br> Lane 2: A549 Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP2 antigen affinity purified polyclonal antibody (Catalog # PB9557) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP2 at approximately 72 kDa. The expected band size for SP2 is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sp4-picoband-trade-antibody-pb9558-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9558-1-WB-anti-sp4-picoband-antibody.jpgAnti-Transcription factor Sp4 Antibody Picoband&reg; Western blot analysis of SP4 using anti-SP4 antibody (PB9558). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: U87 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP4 antigen affinity purified polyclonal antibody (Catalog # PB9558) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP4 at approximately 82 kDa. The expected band size for SP4 is at 82 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sparc-picoband-trade-antibody-pb9559-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9559-1-WB-anti-sparc-picoband-antibody.jpgAnti-SPARC Antibody Picoband&reg; Western blot analysis of SPARC using anti-SPARC antibody (PB9559). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug,<br> Lane 2: 293T Whole Cell Lysate at 40ug,<br> Lane 3: MCF-7 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # PB9559) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stathmin-1-picoband-trade-antibody-pb9560-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-stmn1-primary-antibodies-wb-testing-1.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; Western blot analysis of Stathmin 1 using anti-Stathmin 1 antibody (PB9560).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.<br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human THP-1 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat thymus tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse thymus tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Stathmin 1 antigen affinity purified polyclonal antibody (Catalog # PB9560) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Stathmin 1 at approximately 17KD. The expected band size for Stathmin 1 is at 17KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-2_1.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; IHC analysis of Stathmin 1 using anti-Stathmin 1 antibody (PB9560). <br> Stathmin 1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Stathmin 1 Antibody (PB9560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-3_1.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; IHC analysis of Stathmin 1 using anti-Stathmin 1 antibody (PB9560). <br> Stathmin 1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Stathmin 1 Antibody (PB9560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-4_1.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; IHC analysis of Stathmin 1 using anti-Stathmin 1 antibody (PB9560). <br> Stathmin 1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Stathmin 1 Antibody (PB9560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-5.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; IHC analysis of Stathmin 1 using anti-Stathmin 1 antibody (PB9560). <br> Stathmin 1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Stathmin 1 Antibody (PB9560) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-6.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; IF analysis of Stathmin 1 using anti-Stathmin 1 antibody (PB9560). <br> Stathmin 1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Stathmin 1 Antibody (PB9560) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-7.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-Stathmin 1 antibody (PB9560).<br>Overlay histogram showing THP-1 cells stained with PB9560 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Stathmin 1 Antibody (PB9560,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-8.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; Flow Cytometry analysis of RAW264.7 cells using anti-Stathmin 1 antibody (PB9560).<br>Overlay histogram showing RAW264.7 cells stained with PB9560 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Stathmin 1 Antibody (PB9560,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9560-9.jpgAnti-Stathmin 1/STMN1 Antibody Picoband&reg; Flow Cytometry analysis of RH-35 cells using anti-Stathmin 1 antibody (PB9560).<br>Overlay histogram showing RH-35 cells stained with PB9560 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Stathmin 1 Antibody (PB9560,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e-cadherin-picoband-trade-antibody-pb9561-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-wb-testing-1.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Western blot analysis of CDH1 using anti-CDH1 antibody (PB9561). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human T47D whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH1 antigen affinity purified polyclonal antibody (PB9561) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CDH1 at approximately 130 kDa. The expected band size for CDH1 is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-ihc-testing-1.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;IHC analysis of CDH1 using anti-CDH1 antibody (PB9561). <br>CDH1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-ihc-testing-2.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;IHC analysis of CDH1 using anti-CDH1 antibody (PB9561). <br>CDH1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-ihc-testing-3.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;IHC analysis of CDH1 using anti-CDH1 antibody (PB9561). <br>CDH1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-ihc-testing-4.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;IHC analysis of CDH1 using anti-CDH1 antibody (PB9561). <br>CDH1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-ihc-testing-5.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;IHC analysis of CDH1 using anti-CDH1 antibody (PB9561). <br>CDH1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-if-testing-1.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;IF analysis of CDH1 using anti-CDH1 antibody (PB9561). <br> CDH1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CDH1 Antibody (PB9561) overnight at 4°C. Fluoro488488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-ijcep0008-12357-f5.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Wnt/β-catenin pathway is involved in CXCL12-induced EMT in MCF-7 cells. A. Relative protein levels of Wnt and β-catenin were detected by western blot, and representative blots were presented including β-actin as the internal control; B. A series of representative illustrations of β-catenin distributions from immunofluorescence analysis are shown. β-catenin was visualized with Cy3-labeled goat anti-rabbit IgG as red. Cells nuclei were stained with DAPI as blue. Scale bars: 200 μm; C. Western blot analysis on the expression of β-catenin and E-cadherin after RNA interference of β-catenin gene in CXCL12 over expressed MCF-7 cells. Representative photographs were shown including β-actin as the internal control. Experiments were performed for three times, and data are expressed as mean ± SD. ** P <0.01, *** P <0.001 vs. parental.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4680367/&orig_host=www.ncbi.nlm.nih.gov'>26722422</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-41598_2017_18869_fig7_html.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Immunohistochemical analysis of dorsolateral prostate E-cadherin, Vimtein, ERα and AR expression in aged rats. The expression of vimentin, ERα and AR increased, and the expression of E-cadherin decreased in BPA-treated groups. ( a – p ) Representative sections of comparable regions are shown for vehicle control rats ( a , e , i , m ), and animals exposed to BPA (10 μg/kg/day) ( b , f , j , n ), BPA (30 μg /kg/day) ( c , g , k , o ), and BPA (90 μg/kg/day) ( d , h , l , p ) (scale bar: 50 μm, ×400). BPA: bisphenol A; AR: androgen receptor; ERα:estrogen receptor-α. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-18869-8'>29323181</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-ijcep0008-12357-f4.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Over expression of CXCL12 promotes EMT of MCF-7 cells. A. Representative photographs of western blot analysis on the expression of E-cadherin, vimentin, N-cadherin, and α-SMA. B. The corresponding densitometric analysis of E-cadherin. C. The corresponding densitometric analysis of vimentin, N-cadherin, and α-SMA. β-actin was served as an internal control; D. Immunofluorescence assay was carried out to address the expression of E-cadherin. Representative photomicrographs are shown. The red fluorescence in cytoplasm was target proteins stained with Cy3-labeled goat anti-rabbit IgG. Nuclei labeled with DAPI were observed to be blue. Scale bars: 200 μm. The above two experiments were repeated for three times, data are presented as mean ± SD. ** P <0.01, *** P <0.001 vs. parental.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4680367/&orig_host=www.ncbi.nlm.nih.gov'>26722422</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-aair-14-233-g002.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;TL1A levels are increased after TGF-β1 treatment in bronchial epithelial cells. (A) Volcano plots were constructed using fold-change values and adjusted P . The red point in the plot represents overexpressed mRNAs and the blue point indicates down-regulated mRNAs with statistical significance. (B) The expression distribution of TNFSF15, where different colors represent different groups and the vertical axis represents the gene expression distribution. (C and D) Protein expression levels of E-cadherin, N-cadherin, fibronectin, DR3, TL1A, and α-SMA after treatment with different concentrations of TGF-β1 for 48 hours. (E) Immunofluorescence for collagen I, fibronectin, and TL1A after treatment with 20 ng/mL TGF-β1 for 48 hours. (F) The RNA levels of E-cadherin, collagen I, N-cadherin, fibronectin, DR3, TL1A, MMP-9. and α-SMA after treatment with different concentrations of TGF-β1 for 48 hours. (G-H) The levels of secreted TL1A remained unchanged in BEAS-2B cells treated with TGF-β1 at various concentrations and during various time courses. Data are expressed as means ± standard deviation. TGF, transforming growth factor; TL1A, tumor necrosis factor ligand-related molecule 1A; DR3, death receptor 3; MMP-9, matrix metallopeptidase 9; α-SMA, α-smooth muscle actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. * P < 0.05, ** P < 0.01 versus the control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8914606/&orig_host=www.ncbi.nlm.nih.gov'>35255540</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-aair-14-233-g004.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Overexpression nsTL1A affects remodeling and EMT induced by TGF-β1. (A) The construction of truncated TL1A plasmid for deletion aa66-94. (B) mRNA expression of TL1A after truncated TL1A plasmid transfection and TGF-β treatment. (B-D) Protein and mRNA expressions of fibronectin, collagen I, N-cadherin E-cadherin and MMP-9 after different treatments. Data are expressed as mean ± standard deviation. nsTL1A, non-secreted tumor necrosis factor ligand-related molecule 1A; EMT, epithelial-mesenchymal transition; TGF, transforming growth factor; MMP-9, matrix metallopeptidase 9; TNF, tumor necrosis factor; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. * P < 0.05, ** P < 0.01 versus the corresponding group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8914606/&orig_host=www.ncbi.nlm.nih.gov'>35255540</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-41419_2022_5482_fig2_html.pngAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;LPC inhibits pulmonary metastasis of CT26 colon cancer. a Schematic view of the experimental procedures of CT26 pulmonary metastatic mouse model. b , c Image and corresponding H&E staining of lung tissue. d Percentage change of body weight. e – g Lung weight, the number of lung tumor nodule, and metastasis rate ( n = 6 mice). h Protein expression of PCNA, vimentin and E-cadherin in lungs ( n = 3 mice). Data were presented as mean ± SEM, * p < 0.05, ** p < 0.01. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-022-05482-5'>36774343</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-41598_2022_23837_fig9_html.pngAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Interfering with DLGAP4 inhibits the PPARβ/δ signalling pathway and the expression of proliferation- and metastasis-related proteins. Western blotting was performed to measure the protein expression of DLGAP4, PPARβ/δ, CyclinD1, E-cadherin and N-cadherin in shNC or shDLGAP4 HepG2 and HCCLM3 cells. The data were obtained from the average of three independent experiments. *P < 0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-022-23837-y'>36396671</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-99188-g006.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;SLC16A8 siRNA reverses the effects of hypoxia on cell endothelial-mesenchymal transition of HUVECs. A and B: The effect of SLC16A8 siRNA on the proliferation of hypoxia treated cells; C and D: SLC16A8 siRNA can affect the migration (C) and invasion (D) of hypoxic HUVECs; E: SLC16A8 siRNA can affect the expression level of E-cadherin, N-cadherin and Vimentin in hypoxia treated cells; F: Effect of SLC16A8 siRNA on the ability of endothelial cells to form tubes after co culture of colorectal cancer cells with hypoxia. a P < 0.05, b P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-41598_2024_75938_fig4_html.pngAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;The combination of AO/854 and CDDP could effectively suppress the ability of metastasis and invasion in PC3 cells. ( A-B ) Inhibitory effects of AO/854 (2.5 µmol/L) and CDDP (2.5 µmol/L) on the migration activity of PC3 cells. Scale bars: 50 μm. ( C-D ) Inhibitory effects of AO/854 (2.5 µmol/L) and CDDP (2.5 µmol/L) on invasion activity of PC3 cells. Scale bars: 50 μm. ( E-F ) The results of WB for E-cadherin, N-cadherin, and vimentin. PC3 cells were treated with AO/854 (2.5 µmol/L) and CDDP (2.5 µmol/L) for 48 h. GAPDH was examined as a loading control. The presented results were representative of experiments repeated at least three times. Data were represented as mean ± SD (* P < 0.05, ** P < 0.01, *** P < 0.001). Original blots are presented in Figs. – . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-75938-5'>39438537</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-99188-g003.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Effect of hypoxia on endothelial-mesenchymal transition of HUVEC co-cultured with colorectal cancer cells. A and B: Effect of hypoxia on cell viability; C and D: Effect of hypoxia on migration (C) and invasion (D) of HUVECs; E: Effect of hypoxia on the ability of endothelial cells’ tube formation; F: Hypoxia affected the expression of E-cadherin, N-cadherin and Vimentin in HUVECs. a P < 0.05, b P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-ijms-26-04630-g007.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;PPI inhibits the metastasis of cervical cancer cells in vivo. ( A , B ) Bioluminescence imaging of various organs exhibiting metastatic or disseminated cancer cells ( A ) and a quantitative analysis of total bioluminescence in each group of organs ( B ). ( C ) H&E staining of various organs in each group. The scale is 100 μm. ( D , E ) The immunofluorescence staining of E-cadherin, VE-cadherin, and Vimentin was performed, followed by data quantification analysis, comparing the control group with the treated group. Six animal samples were used per group ( n = 6), and the results are presented as mean ± S.D. **: p < 0.01; ***: p < 0.001 vs. control. The scale is 100 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12110821/'>40429774</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-ijms-26-04630-g004.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;PPI inhibits the growth, migration, and invasion of HO-8910PM cells by reversing the EMT progress. ( A ) Cell proliferation was evaluated at 48 h using the MTT assay, and the IC 50 values were determined from dose–response curves generated with GraphPad Prism 8. ( B ) The wound-healing assay was conducted in HO-8910PM cells to evaluate cell migration. Images were captured at 0 and 24 h. The scale is 500 μm. ( C ) The data quantification results of ( B ). ( D ) A transwell migration assay was performed to monitor the rate of cellular migration. The scale is 500 μm. ( E ) The quantification results of ( D ). ( F ) The protein expression levels of E-cadherin, VE-cadherin, and Vimentin in HO-8910PM cells. ( G ) The quantification results of ( F ). ( H ) An immunofluorescence assay was performed to detect E-cadherin, VE-cadherin, and Vimentin expression in HO-8910PM cells treated with PPI. The scale is 100 μm. ( I ) The quantification results of ( H ). Data from six independent experiments are presented as mean ± S.D. *: p < 0.05; **: p < 0.01; ***: p < 0.001 vs. control.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12110821/'>40429774</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-wjg-18-7070-g002.jpgAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Expression of connective tissue growth factor mRNA and its association with epithelial-mesenchymal transition in hepatocellular carcinoma. Connective tissue growth factor (CCN2) mRNA (A) or protein (B) were detected in hepatoma cells at the edge of carcinoma foci by in situ hybridization or immunohistochemistry respectively; C: E-cadherin was only weakly detectable in carcinoma foci but stained more intensely in normal hepatocytes; D, E: Fibroblast-specific protein-1 (FSP-1)-positive hepatoma cells (white arrow) were located at a position between the edge of carcinoma foci and vascular wall; FSP-1-positive (black arrows) (D) or (E) α-smooth muscle actin-positive (black arrows) fibroblast-like or myofibroblast-like hepatic stellate cell (HSC) were found within carcinoma foci; F-H: CCN2 mRNA was detected in either fibroblast-like or myofibroblast-like HSC (black arrows); CCN2 mRNA (G) or (H) FSP-1 were found in some hepatoma cells. Original magnification, × 400 in A, B, C, D, F and G, × 200 in E and H. Examples of positively stained cells or structures in each panel are arrowed.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC3531696/&orig_host=www.ncbi.nlm.nih.gov'>23323010</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9561-cdh1-primary-antibodies-wb-review-1.pngAnti-E Cadherin 1/CDH1 Antibody Picoband&reg;Western blot analysis of CDH1 using anti-CDH1 antibody (PB9561). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human cervical cancer tissue lysates, <br> Lane 2: human cervical cancer adjacent tissue lysates, <br> Lane 3: human cervical cancer tissue lysates, <br> Lane 4: human cervical cancer adjacent tissue lysates. <br> Lane 5: human cervical cancer tissue lysates, <br> Lane 6: human cervical cancer adjacent tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH1 antigen affinity purified polyclonal antibody (PB9561) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for CDH1 at approximately 130 kDa. The expected band size for CDH1 is at 98 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcsf-picoband-trade-antibody-pb9562-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9562-1-IHC-anti-m-csf-antibody.jpgAnti-MCSF/CSF1 Antibody IHC analysis of MCSF using anti-MCSF antibody (PB9562). <br> MCSF was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCSF Antibody (PB9562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9562-2-IHC-anti-m-csf-antibody.jpgAnti-MCSF/CSF1 Antibody IHC analysis of MCSF using anti-MCSF antibody (PB9562). <br> MCSF was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MCSF Antibody (PB9562) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-g-csf-picoband-trade-antibody-pb9563-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9563-g-csf-primary-antibodies-wb-testing-1.jpgAnti-G-CSF/CSF3 Antibody Picoband&reg; Western blot analysis of G-CSF/CSF3 using anti-G-CSF/CSF3 antibody (PB9563). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human MOLT-4 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G-CSF/CSF3 antigen affinity purified polyclonal antibody (Catalog # PB9563) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G-CSF/CSF3 at approximately 22 kDa. The expected band size for G-CSF/CSF3 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9563-g-csf-primary-antibodiesihc-testing-2.jpgAnti-G-CSF/CSF3 Antibody Picoband&reg; IHC analysis of G-CSF/CSF3 using anti-G-CSF/CSF3 antibody (PB9563). <br> G-CSF/CSF3 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G-CSF/CSF3 Antibody (PB9563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9563-g-csf-primary-antibodiesihc-testing-3.jpgAnti-G-CSF/CSF3 Antibody Picoband&reg; IHC analysis of G-CSF/CSF3 using anti-G-CSF/CSF3 antibody (PB9563). <br> G-CSF/CSF3 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G-CSF/CSF3 Antibody (PB9563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9563-g-csf-primary-antibodiesihc-testing-4.jpgAnti-G-CSF/CSF3 Antibody Picoband&reg; IHC analysis of G-CSF/CSF3 using anti-G-CSF/CSF3 antibody (PB9563). <br> G-CSF/CSF3 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-G-CSF/CSF3 Antibody (PB9563) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cystatin-a-picoband-trade-antibody-pb9564-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9564-csta-primary-antibodies-wb-testing-1.jpgAnti-Cystatin A/CSTA Antibody Picoband&reg;Western blot analysis of Cystatin A using anti-Cystatin A antibody (PB9564). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat stomach tissue lysates,<br> Lane 5: mouse stomach tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystatin A antigen affinity purified polyclonal antibody (Catalog # PB9564) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cystatin A at approximately 11 kDa. The expected band size for Cystatin A is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9564-csta-primary-antibodies-if-testing-1.jpgAnti-Cystatin A/CSTA Antibody Picoband&reg;IF analysis of Cystatin A using anti-Cystatin A antibody (PB9564) and anti-Tubulin Alpha antibody (M03989-3).<br> Cystatin A was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cystatin A Antibody (PB9564) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stefin-b-picoband-trade-antibody-pb9565-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9565-cstb-primary-antibodies-wb-testing-1.jpgAnti-Stefin B/CSTB Antibody Picoband&reg; Western blot analysis of Stefin B using anti-Stefin B antibody (PB9565). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A431 whole cell lysates.<br> Lane 3: human A549 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Stefin B antigen affinity purified polyclonal antibody (Catalog # PB9565) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Stefin B at approximately 11 kDa. The expected band size for Stefin B is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9565-2-IHC-anti-cystatin-b-antibody.jpgAnti-Stefin B/CSTB Antibody Picoband&reg; IHC analysis of Stefin B using anti-Stefin B antibody (PB9565). <br> Stefin B was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Stefin B Antibody (PB9565) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9565-cstb-primary-antibodies-if-testing-3.jpgAnti-Stefin B/CSTB Antibody Picoband&reg; IF analysis of Stefin B using anti-Stefin B antibody (PB9565). <br> Stefin B was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Stefin B Antibody (PB9565) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9565-4.pngAnti-Stefin B/CSTB Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Stefin B antibody (PB9565). <br> Overlay histogram showing U20S cells stained with Stefin B (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Stefin B Antibody (Stefin B&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9565-5.pngAnti-Stefin B/CSTB Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-Stefin B antibody (PB9565). <br> Overlay histogram showing A431 cells stained with Stefin B (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Stefin B Antibody (Stefin B&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-baff-picoband-trade-antibody-pb9567-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9567-1-WB-anti-baff-picoband-antibody.jpgAnti-BAFF/TNFSF13B Antibody Picoband&reg; Western blot analysis of BAFF using anti-BAFF antibody (PB9567). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Kidney Tissue Lysate at 50ug,<br> Lane 2: Rat Spleen Tissue Lysate at 50ug,<br> Lane 3: Mouse Spleen Tissue Lysate at 50ug,<br> Lane 4: Human Placenta Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF antigen affinity purified polyclonal antibody (Catalog # PB9567) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF at approximately 31 kDa. The expected band size for BAFF is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp7a1-antibody-rp1079-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1079-cyp7a1-primary-antibodies-wb-testing-1.jpgAnti-CYP7A1 Antibody Picoband&reg; Western blot analysis of CYP7A1 using anti-CYP7A1 antibody (RP1079). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: rat RH35 whole clel lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP7A1 antigen affinity purified polyclonal antibody (Catalog # RP1079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP7A1 at approximately 58 kDa. The expected band size for CYP7A1 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1079-cyp7a1-primary-antibodies-fcm-testing-2.jpgAnti-CYP7A1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-CYP7A1 antibody (RP1079). <br>Overlay histogram showing SiHa cells stained with RP1079 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP7A1 Antibody (RP1079, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-osteopontin-antibody-rp1080-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1080-1_1.jpgAnti-Osteopontin/SPP1 Antibody Picoband&reg; Western blot analysis of Osteopontin using anti-Osteopontin antibody (RP1080). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates&#44; <br> Lane 3: human SHG-44 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin antigen affinity purified polyclonal antibody (Catalog # RP1080) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Osteopontin at approximately 66&#44;25-55KD. The expected band size for Osteopontin is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1080-40364_2025_746_fig7_html.pngAnti-Osteopontin/SPP1 Antibody Picoband&reg;Mononuclear phagocytic system lineage analysis reveals the potential function of SPP1 + Mac enriched in LFH. (A) UMAP of MPs in human LF tissue, dyed according to the cell types. SPP1 + Mac are circled by the black dotted line. (B) Dot plot displaying marker gene expression in different mononuclear phagocyte subpopulations, with color indicating the scaled mean expression of genes. (C) UMAP of MPs from the non-LFH group and the LFH group, dyed according to the cell types. (D) Bar graph showing the proportion of each mononuclear phagocyte subpopulation in the non-LFH group and the LFH group. (E) Stacked violin plot showing the expression of common markers in SPP1 + Mac and IL1B + Mac, categorized into Mø, M1, and M2 types. (F) Representative IHC images showing the expression of CD86, CD206, and SPP1 in non-LFH and LFH samples. Scale bar, 50 μm. Data quantification results are presented on the right, as mean ± SD, with * p < 0.05, ** p < 0.01, and *** p < 0.001. (G) UMAP plots showing M1 (left) and M2 (right) scoring results for each mononuclear phagocyte cluster, with yellow representing high scores and purple representing low scores. (H) Heatmap revealing GSVA scoring results for each mononuclear phagocyte cluster, with red indicating high scores and blue indicating low scores <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40364-025-00746-6'>40001138</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1080-40364_2025_746_fig8_html.pngAnti-Osteopontin/SPP1 Antibody Picoband&reg;Deciphering the complex interactions among multiple cell lineages in the fibrotic microenvironment of LFH. (A) Circos plots showing potential interactions between High Ferro-score FB and 15 immune cell clusters in the non-LFH (left) and LFH groups (right). Edge width indicates the number of significant L-R pairs between cell types. (B) Interaction strengths for incoming and outgoing signaling events among all clusters in non-LFH and LFH groups. The horizontal axis represents outgoing interaction strength, while the vertical axis represents incoming interaction strength. (C) Bubble plot illustrating communication probabilities of L-R interactions between SPP1 + Mac or IL1B + Mac subclusters (sending signals) and High Ferro-score FB subcluster (receiving signals) in the up-regulated signaling pathways of the LFH group.Red characters represent ligands, and purple characters represent receptors. Bubble color and size represent calculated communication probabilities and p -values, respectively. (D) Comparison of the number and strength of inferred cellular interactions in the non-LFH and LFH groups. (E) Bubble connectivity plot displaying upregulated receptor-ligand pairs from signaling pathways in the LFH group, and their expression levels in corresponding cell clusters. The color of the bubble reflects the communication probability, and the bubble size indicates the percentage expression of L-R pairs. (F) Circle plot of inferred SPP1 signaling networks among SPP1 + Mac and other cell clusters. (G) Bar graph showing the distribution of L-R pairs between SPP1 + Mac and High Ferro-score FB. (H) Violin plots showing the expression difference of ligand SPP1 between SPP1 + Mac in non-LFH and LFH groups, and of receptor CD44 expression in High Ferro-score FB between non-LFH and LFH groups. **** p < 0.0001. ( I - J ) Representative multiplex immunofluorescent images of LF tissue from non-LFH and LFH groups showing the expression of SPP1 (red), CD44 (green), COL1 (orange), and FTL (purple). Nuclei are labeled with DAPI (blue). Scale bar, left, 100 μm; bottom, 100 μm; right, 20 μm <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s40364-025-00746-6'>40001138</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1080-thnov14p4822g004.jpgAnti-Osteopontin/SPP1 Antibody Picoband&reg;Spatial co-localization of TAM with FAP + CAF. (A) UMAP shows the distribution of monocyte/macrophage subtypes. (B) Bar plot shows the relative proportions of macrophage subtypes in HCC and ICC samples (left); paired dot plot shows the relative proportions of DAB2 + / SPP1 + TAMs in tumor and adjacent liver paired samples (right). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. (C) Distribution of DAB2 + TAMs and FAP + CAF in HCC boundary slides, and SPP1 + TAM and FAP + CAF in ICC boundary slides based on CellTrek deconvolution (left); heatmap shows the Kullback-Leibler (KL) divergence of FAP + CAF with different macrophage subtypes in ST slides, with the higher KL divergence representing the greater degree of co-localization of the two cell types (right). (D) Pie plots showing the relative proportions of different macrophage subtypes in the ST slides. (E) Unbiased clustering of ST spots and definition of cell types of each cluster (left); dot plot showing the expression of select marker genes of each cluster (right). (F) Multi-plex immunofluorescence images showing the aggregation of FAP + CAF with DAB2 + TAM at the tumor border in HCC and FAP + CAF with SPP1 + TAM at the tumor border and core in ICC. The scale bar is 200 μm and 100 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC11373629/&orig_host=www.ncbi.nlm.nih.gov'>39239526</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fetuin-a-picoband-trade-antibody-pb9568-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9568-ahsg-primary-antibodies-wb-testing-1.jpgAnti-AHSG Antibody Picoband&reg; Western blot analysis of AHSG using anti-AHSG antibody (PB9568). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HCCT tissue lysates, <br> Lane 3: human HCCP tissue lysates, <br> Lane 4: rat plasma lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHSG antigen affinity purified polyclonal antibody (Catalog # PB9568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AHSG at approximately 55-60 kDa. The expected band size for AHSG is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9568-2-IHC-anti-fetuin-a-antibody.jpgAnti-AHSG Antibody Picoband&reg; IHC analysis of AHSG using anti-AHSG antibody (PB9568). <br> AHSG was detected in paraffin-embedded section of Human Liver Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AHSG Antibody (PB9568) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9568-fetuin_a-primary-antibodies-fc-testing-3.jpgAnti-AHSG Antibody Picoband&reg;3. Flow Cytometry analysis of HEPG2 cells using anti-Fetuin A antibody (PB9568). <br>Overlay histogram showing HEPG2 cells stained with PB9568 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Fetuin A Antibody (PB9568&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eotaxin-picoband-trade-antibody-pb9569-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9569-ccl11-primary-antibodies-wb-testing-1.jpgAnti-Eotaxin/CCL11 Antibody Picoband&reg;Western blot analysis of Eotaxin/CCL11 using anti-Eotaxin/CCL11 antibody (PB9569). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant human Eotaxin/CCL11 protein 5ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin/CCL11 antigen affinity purified polyclonal antibody (PB9569) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Eotaxin/CCL11 at approximately 11 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9569-ccl11-primary-antibodies-wb-testing-2.jpgAnti-Eotaxin/CCL11 Antibody Picoband&reg;Western blot analysis of Eotaxin/CCL11 using anti-Eotaxin/CCL11 antibody (PB9569). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant mouse Eotaxin/CCL11 protein 5ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin/CCL11 antigen affinity purified polyclonal antibody (PB9569) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Eotaxin/CCL11 at approximately 11 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9569-ccl11-primary-antibodies-wb-testing-3.jpgAnti-Eotaxin/CCL11 Antibody Picoband&reg;Western blot analysis of Eotaxin/CCL11 using anti-Eotaxin/CCL11 antibody (PB9569). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant rat Eotaxin/CCL11 protein 10ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eotaxin/CCL11 antigen affinity purified polyclonal antibody (PB9569) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Eotaxin/CCL11 at approximately 15 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9569-ccl11-primary-antibodies-ihc-testing-1.jpgAnti-Eotaxin/CCL11 Antibody Picoband&reg;IHC analysis of Eotaxin/CCL11 using anti-Eotaxin/CCL11 antibody (PB9569). <br> Eotaxin/CCL11 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Eotaxin/CCL11 Antibody (PB9569) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcp-1-picoband-trade-antibody-pb9570-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9570-1-WB-anti-mcp-1-antibody.jpgAnti-MCP1/CCL2 Antibody Picoband&reg; Western blot analysis of MCP-1 using anti-MCP-1 antibody (PB9570). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: SW620 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCP-1 antigen affinity purified polyclonal antibody (Catalog # PB9570) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCP-1 at approximately 11 kDa. The expected band size for MCP-1 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9570-ccl2-primary-antibodies-ihc-testing-2.jpgAnti-MCP1/CCL2 Antibody Picoband&reg; IHC analysis of MCP1/CCL2 using anti-MCP1/CCL2 antibody (PB9570). <br> MCP1/CCL2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCP1/CCL2 Antibody (PB9570) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9570-ccl2-primary-antibodies-ihc-testing-3.jpgAnti-MCP1/CCL2 Antibody Picoband&reg; IHC analysis of MCP1/CCL2 using anti-MCP1/CCL2 antibody (PB9570). <br> MCP1/CCL2 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MCP1/CCL2 Antibody (PB9570) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccl3-picoband-trade-antibody-pb9571-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9571-1-WB-anti-ccl3-mip-1alpha-picoband-antibody.jpgAnti-CCL3/Macrophage Inflammatory Protein 1 alpha Antibody Picoband&reg; Western blot analysis of CCL3 using anti-CCL3 antibody (PB9571). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: JURKAT Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9571) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 11 kDa. The expected band size for CCL3 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccl4-mip-1-beta-picoband-trade-antibody-pb9572-boster.html2026-03-24T05:05:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9572-1-WB-anti-ccl4-mip-1-beta-antibody.jpgAnti-Macrophage Inflammatory Protein 1 beta/CCL4 Antibody Picoband&reg; Western blot analysis of CCL4 using anti-CCL4 antibody (PB9572). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL4 antigen affinity purified polyclonal antibody (Catalog # PB9572) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL4 at approximately 11 kDa. The expected band size for CCL4 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rantes-picoband-trade-antibody-pb9573-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9573-1-WB-anti-rantes-picoband-antibody.jpgAnti-RANTES/CCL5 Antibody Picoband&reg; Western blot analysis of RANTES using anti-RANTES antibody (PB9573). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HUT Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANTES antigen affinity purified polyclonal antibody (Catalog # PB9573) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANTES at approximately 11 kDa. The expected band size for RANTES is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc37-picoband-trade-antibody-pb9574-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9574-1_1-WB-anti-cdc37-picoband-antibody.jpgAnti-Cdc37 Antibody Picoband&reg; Western blot analysis of Cdc37 using anti-Cdc37 antibody (PB9574). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: 293T Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc37 antigen affinity purified polyclonal antibody (Catalog # PB9574) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc37 at approximately 44 kDa. The expected band size for Cdc37 is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apolipoprotein-j-picoband-trade-antibody-pb9575-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9575-1-WB-anti-clusterin-antibody.jpgAnti-Apolipoprotein J/CLU Antibody Picoband&reg; Western blot analysis of Apolipoprotein J using anti-Apolipoprotein J antibody (PB9575). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SKOV Whole Cell Lysate at 40ug,<br> Lane 2: U87 Whole Cell Lysate at 40ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein J antigen affinity purified polyclonal antibody (Catalog # PB9575) at 0.5 &mu;g/mL overnight at 4&deg;C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein J at approximately 82 kDa. The expected band size for Apolipoprotein J is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9575-2-IHC-anti-clusterin-antibody.jpgAnti-Apolipoprotein J/CLU Antibody Picoband&reg; IHC analysis of Apolipoprotein J using anti-Apolipoprotein J antibody (PB9575). <br> Apolipoprotein J was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Apolipoprotein J Antibody (PB9575) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cry2-picoband-trade-antibody-pb9576-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9576-1-WB-anti-cry2-picoband-antibody.jpgAnti-CRY2 Antibody Picoband&reg; Western blot analysis of CRY2 using anti-CRY2 antibody (PB9576). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Rat Testis Tissue Lysate at 50ug,<br> Lane 2: Rat Brain Tissue Lysate at 50ug,<br> Lane 3: Mouse Brain Tissue Lysate at 50ug,<br> Lane 4: 22RV1 Whole Cell Lysate at 40ug. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRY2 antigen affinity purified polyclonal antibody (Catalog # PB9576) at 0.5 &mu;g/mL overnight at 4&deg;C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRY2 at approximately 67 kDa. The expected band size for CRY2 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9576-2-IHC-anti-cry2-picoband-antibody.jpgAnti-CRY2 Antibody Picoband&reg; IHC analysis of CRY2 using anti-CRY2 antibody (PB9576). <br> CRY2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CRY2 Antibody (PB9576) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9576-cry2-primary-antibodies-ihc-testing-4.jpgAnti-CRY2 Antibody Picoband&reg;IHC analysis of Cryptochrome 2/CRY2 using anti-Cryptochrome 2/CRY2 antibody (PB9576). <br>Cryptochrome 2/CRY2 was detected in a paraffin-embedded section of human heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cryptochrome 2/CRY2 Antibody (PB9576) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9576-3-IHC-anti-cry2-picoband-antibody.jpgAnti-CRY2 Antibody Picoband&reg; IHC analysis of CRY2 using anti-CRY2 antibody (PB9576). <br> CRY2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CRY2 Antibody (PB9576) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-g-csf-picoband-trade-antibody-pb9577-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9577-1-WB-anti-g-csf-picoband-antibody.jpgAnti-G-CSF/CSF3 Antibody Picoband&reg; Western blot analysis of G-CSF using anti-G-CSF antibody (PB9577). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G-CSF antigen affinity purified polyclonal antibody (Catalog # PB9577) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G-CSF at approximately 22 kDa. The expected band size for G-CSF is at 22 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddb1-picoband-trade-antibody-pb9578-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-wb-testing-1.jpgAnti-DDB1 Antibody Picoband&reg; Western blot analysis of DDB1 using anti-DDB1 antibody (PB9578). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human HEK293 whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: human Raji whole cell lysates, <br> Lane 6: human CACO-2 whole cell lysates, <br> Lane 7: human THP-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB1 antigen affinity purified polyclonal antibody (Catalog # PB9578) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB1 at approximately 127 kDa. The expected band size for DDB1 is at 127 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-wb-testing-2.jpgAnti-DDB1 Antibody Picoband&reg; Western blot analysis of DDB1 using anti-DDB1 antibody (PB9578). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat kidney tissue lysates, <br> Lane 3: rat C6 whole cell lysates, <br> Lane 4: mouse brain tissue lysates, <br> Lane 5: mouse kidney tissue lysates, <br> Lane 6: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB1 antigen affinity purified polyclonal antibody (Catalog # PB9578) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB1 at approximately 127 kDa. The expected band size for DDB1 is at 127 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-fcm-testing-11.jpgAnti-DDB1 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-DDB1 antibody (PB9578).<br>Overlay histogram showing 293T cells stained with PB9578 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDB1 Antibody (PB9578,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-3-ihc-anti-ddb1-picoband-antibody.jpgAnti-DDB1 Antibody Picoband&reg; IHC analysis of DDB1 using anti-DDB1 antibody (PB9578).<br> DDB1 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-4-ihc-anti-ddb1-picoband-antibody.jpgAnti-DDB1 Antibody Picoband&reg; IHC analysis of DDB1 using anti-DDB1 antibody (PB9578).<br> DDB1 was detected in paraffin-embedded section of Rat Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-5-ihc-anti-ddb1-picoband-antibody.jpgAnti-DDB1 Antibody Picoband&reg; IHC analysis of DDB1 using anti-DDB1 antibody (PB9578).<br> DDB1 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-ihc-testing-6_1.jpgAnti-DDB1 Antibody Picoband&reg; IHC analysis of DDB1 using anti-DDB1 antibody (PB9578).<br> DDB1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-ihc-testing-7_1.jpgAnti-DDB1 Antibody Picoband&reg; IHC analysis of DDB1 using anti-DDB1 antibody (PB9578).<br> DDB1 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-ihc-testing-8_1.jpgAnti-DDB1 Antibody Picoband&reg; IHC analysis of DDB1 using anti-DDB1 antibody (PB9578).<br> DDB1 was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-ihc-testing-9.jpgAnti-DDB1 Antibody Picoband&reg; IHC analysis of DDB1 using anti-DDB1 antibody (PB9578).<br> DDB1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9578-ddb1-primary-antibodies-if-testing-10.jpgAnti-DDB1 Antibody Picoband&reg; IF analysis of DDB1 using anti-DDB1 antibody (PB9578). <br> DDB1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDB1 Antibody (PB9578) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dld-picoband-trade-antibody-pb9579-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9579-2-IHC-anti-dld-picoband-antibody.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; IHC analysis of DLD using anti-DLD antibody (PB9579). <br> DLD was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DLD Antibody (PB9579) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9579-dld-primary-antibodies-wb-testing-1_2.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; Western blot analysis of DLD using anti-DLD antibody (PB9579). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates,<br> Lane 4: human U251 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat heart tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLD antigen affinity purified polyclonal antibody (Catalog # PB9579) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DLD at approximately 54 kDa. The expected band size for DLD is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9579-dld-primary-antibodies-icc-testing-3.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; IF analysis of DLD using anti-DLD antibody (PB9579). <br> DLD was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DLD Antibody (PB9579) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9579-dld-primary-antibodies-fcm-testing-4.pngAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-DLD antibody (PB9579). <br>Overlay histogram showing A549 cells stained with PB9579 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DLD Antibody (PB9579, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9579-dld-primary-antibodies-wb-testing-5.jpgAnti-Lipoamide Dehydrogenase/DLD Antibody Picoband&reg; Western blot analysis of DLD using anti-DLD antibody (PB9579). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human SiHa whole cell lysates, <br> Lane 4: human U251 whole cell lysates, <br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat heart tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DLD antigen affinity purified polyclonal antibody (PB9579) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for DLD at approximately 54 kDa. The expected band size for DLD is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eed-picoband-trade-antibody-pb9581-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9581-1-WB-anti-eed-picoband-antibody.jpgAnti-EED Antibody Picoband&reg; Western blot analysis of EED using anti-EED antibody (PB9581). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 2: Rat Kidney Tissue Lysate at 50ug,<br> Lane 3: Rat Liver Tissue Lysate at 50ug,<br> Lane 4: Rat Lung Tissue Lysate at 50ug,<br> Lane 5: SKOV Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EED antigen affinity purified polyclonal antibody (Catalog # PB9581) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EED at approximately 72 kDa. The expected band size for EED is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif6-picoband-trade-antibody-pb9582-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9582-eif6-primary-antibodies-wb-testing-1.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg; Western blot analysis of EIF6 using anti-EIF6 antibody (PB9582). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human 293T whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: mouse liver tissue lysates, <br> Lane 7: mouse RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF6 antigen affinity purified polyclonal antibody (Catalog # PB9582) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF6 at approximately 26 kDa. The expected band size for EIF6 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9582-2-IHC-anti-eif6-picoband-antibody.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg; IHC analysis of EIF6 using anti-EIF6 antibody (PB9582).<br> EIF6 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF6 Antibody (PB9582) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9582-3-IHC-anti-eif6-picoband-antibody.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg; IHC analysis of EIF6 using anti-EIF6 antibody (PB9582).<br> EIF6 was detected in paraffin-embedded section of Rat Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF6 Antibody (PB9582) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9582-4-IHC-anti-eif6-picoband-antibody.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg; IHC analysis of EIF6 using anti-EIF6 antibody (PB9582).<br> EIF6 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF6 Antibody (PB9582) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9582-eif6-primary-antibodies-ihc-testing-5.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg; IHC analysis of EIF6 using anti-EIF6 antibody (PB9582).<br> EIF6 was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EIF6 Antibody (PB9582) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9582-eif6-primary-antibodies-ihc-testing-6.jpgAnti-integrin beta 4 binding protein/EIF6 Antibody Picoband&reg; IHC analysis of EIF6 using anti-EIF6 antibody (PB9582).<br> EIF6 was detected in immunocytochemical section of SW480 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-EIF6 Antibody (PB9582) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eph-receptor-a5-picoband-trade-antibody-pb9583-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9583-1-WB-anti-eph-receptor-a5-picoband-antibody.jpgAnti-Eph receptor A5/EPHA5 Antibody Picoband&reg; Western blot analysis of Eph receptor A5 using anti-Eph receptor A5 antibody (PB9583). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug<br> Lane 2: Mouse Brain Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A5 antigen affinity purified polyclonal antibody (Catalog # PB9583) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A5 at approximately 114 kDa. The expected band size for Eph receptor A5 is at 114 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eph-receptor-b1-picoband-trade-antibody-pb9584-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9584-ephb1-primary-antibodies-wb-testing-1.jpgAnti-Eph receptor B1/EPHB1 Antibody Picoband&reg; Western blot analysis of Eph receptor B1 using anti-Eph receptor B1 antibody (PB9584). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates, <br> Lane 2: human Hel whole cell lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: mouse testis tissue lysates, <br> Lane 6: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor B1 antigen affinity purified polyclonal antibody (Catalog # PB9584) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor B1 at approximately 120 kDa. The expected band size for Eph receptor B1 is at 110 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9584-2-IHC-anti-eph-receptor-b1-picoband-antibody.jpgAnti-Eph receptor B1/EPHB1 Antibody Picoband&reg; IHC analysis of Eph receptor B1 using anti-Eph receptor B1 antibody (PB9584). <br> Eph receptor B1 was detected in paraffin-embedded section of Human Glioma Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Eph receptor B1 Antibody (PB9584) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9584-3.pngAnti-Eph receptor B1/EPHB1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Eph receptor B1 antibody (PB9584). <br> Overlay histogram showing U20S cells stained with PB9584 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Eph receptor B1 Antibody (PB9584&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ewsr1-picoband-trade-antibody-pb9585-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9585-ewsr1-primary-antibodies-wb-testing-1.jpgAnti-EWSR1 Antibody Picoband&reg; Western blot analysis of EWSR1 using anti-EWSR1 antibody (PB9585). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human HL-60 whole cell lysates, <br> Lane 5: human HEK293 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EWSR1 antigen affinity purified polyclonal antibody (Catalog # PB9585) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EWSR1 at approximately 95 kDa. The expected band size for EWSR1 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9585-2-IHC-anti-ewsr1-picoband-antibody.jpgAnti-EWSR1 Antibody Picoband&reg; IHC analysis of EWSR1 using anti-EWSR1 antibody (PB9585). <br> EWSR1 was detected in paraffin-embedded section of Mouse Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9585-3-IHC-anti-ewsr1-picoband-antibody.jpgAnti-EWSR1 Antibody Picoband&reg; IHC analysis of EWSR1 using anti-EWSR1 antibody (PB9585). <br> EWSR1 was detected in paraffin-embedded section of Rat Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9585-4-IHC-anti-ewsr1-picoband-antibody.jpgAnti-EWSR1 Antibody Picoband&reg; IHC analysis of EWSR1 using anti-EWSR1 antibody (PB9585). <br> EWSR1 was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9585-5.jpgAnti-EWSR1 Antibody Picoband&reg; IHC analysis of EWSR1 using anti-EWSR1 antibody (PB9585).<br> EWSR1 was detected in frozen section of mouse intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9585-6.jpgAnti-EWSR1 Antibody Picoband&reg; IHC analysis of EWSR1 using anti-EWSR1 antibody (PB9585).<br> EWSR1 was detected in frozen section of rat intestine tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9585-7.jpgAnti-EWSR1 Antibody Picoband&reg; IHC analysis of EWSR1 using anti-EWSR1 antibody (PB9585).<br> EWSR1 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9585-8.jpgAnti-EWSR1 Antibody Picoband&reg; IHC analysis of EWSR1 using anti-EWSR1 antibody (PB9585).<br> EWSR1 was detected in immunocytochemical section of human SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9585-9.jpgAnti-EWSR1 Antibody Picoband&reg; IF analysis of EWSR1 using anti-EWSR1 antibody (PB9585).<br> EWSR1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-EWSR1 Antibody (PB9585) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9585-10.pngAnti-EWSR1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-EWSR1 antibody (PB9585). <br> Overlay histogram showing U20S cells stained with PB9585 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EWSR1 Antibody (PB9585&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-liver-fabp-picoband-trade-antibody-pb9586-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9586-fabp1-primary-antibodies-wb-testing-1.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg; Western blot analysis of liver FABP using anti-liver FABP antibody (PB9586). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: chicken liver tissue lysates, <br> Lane 3: monkey liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-liver FABP antigen affinity purified polyclonal antibody (Catalog # PB9586) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for liver FABP at approximately 14 kDa. The expected band size for liver FABP is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9586-2-IHC-anti-liver-fabp-picoband-antibody.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg; IHC analysis of liver FABP using anti-liver FABP antibody (PB9586). <br> liver FABP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-liver FABP Antibody (PB9586) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9586-3-IHC-anti-liver-fabp-picoband-antibody.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg; IHC analysis of liver FABP using anti-liver FABP antibody (PB9586). <br> liver FABP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-liver FABP Antibody (PB9586) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9586-4-IHC-anti-liver-fabp-picoband-antibody.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg; IHC analysis of liver FABP using anti-liver FABP antibody (PB9586). <br> liver FABP was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-liver FABP Antibody (PB9586) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9586-fabp1-primary-antibodies-wb-testing-5_1.jpgAnti-liver FABP/FABP1 Antibody Picoband&reg; Western blot analysis of liver FABP using anti-liver FABP antibody (PB9586). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat small intestine tissue lysates, <br> Lane 3: rat RH35 whole cell lysates, <br> Lane 4: rat PC-12 whole cell lysates, <br> Lane 5: mouse liver tissue lysates, <br> Lane 6: mouse small intestine tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-liver FABP antigen affinity purified polyclonal antibody (Catalog # PB9586) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for liver FABP at approximately 14 kDa. The expected band size for liver FABP is at 14 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf2-picoband-trade-antibody-pb9587-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9587-fgf2-primary-antibodies-wb-testing-1.jpgAnti-FGF2 Antibody Picoband&reg; Western blot analysis of FGF2 using anti-FGF2 antibody (PB9587). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SKOV3 whole cell lysates,<br> Lane 3: rat liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (Catalog # PB9587) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 18, 20 kDa. The expected band size for FGF2 is at 18, 22, 30 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-flt-3ligand-picoband-trade-antibody-pb9588-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9588-1-WB-anti-flt-3ligand-antibody.jpgAnti-Flt3 ligand/FLT3LG Antibody Picoband&reg; Western blot analysis of Flt-3ligand using anti-Flt-3ligand antibody (PB9588). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Spleen Tissue Lysate at 50ug,<br> Lane 3: Rat Kidney Tissue Lysate at 50ug,<br> Lane 4: SMMC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt-3ligand antigen affinity purified polyclonal antibody (Catalog # PB9588) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flt-3ligand at approximately 26 kDa. The expected band size for Flt-3ligand is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fmo1-picoband-trade-antibody-pb9589-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9589-fmo1-primary-antibodies-wb-testing-1.jpgAnti-FMO1 Antibody Picoband&reg; Western blot analysis of FMO1 using anti-FMO1 antibody (PB9589). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human HUH7 whole cell lysates,<br> Lane 4: rat liver tissue lysates,<br> Lane 5: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO1 antigen affinity purified polyclonal antibody (PB9589) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FMO1 at approximately 60 kDa. The expected band size for FMO1 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fmo3-picoband-trade-antibody-pb9590-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9590-fmo3-primary-antibodies-wb-testing-1.jpgAnti-FMO3 Antibody Picoband&reg; Western blot analysis of FMO3 using anti-FMO3 antibody (PB9590). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, <br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates, <br> Lane 3: rat liver tissue lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO3 antigen affinity purified polyclonal antibody (Catalog # PB9590) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO3 at approximately 60 kDa. The expected band size for FMO3 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fra2-picoband-trade-antibody-pb9591-boster.html2026-03-24T05:05:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9591-1-WB-anti-fra2-picoband-antibody.jpgAnti-FRA2/FOSL2 Antibody Picoband&reg; Western blot analysis of FRA2 using anti-FRA2 antibody (PB9591). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Lung Tissue Lysate at 50ug,<br> Lane 2: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug,<br> Lane 4: SW620 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA2 antigen affinity purified polyclonal antibody (Catalog # PB9591) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA2 at approximately 43 kDa. The expected band size for FRA2 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fascin-picoband-trade-antibody-pb9592-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9592-fscn1-primary-antibodies-wb-testing-1_1.jpgAnti-Fascin/FSCN1 Antibody Picoband&reg; Western blot analysis of Fascin/FSCN1 using anti-Fascin/FSCN1 antibody (PB9592). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human HepG2 whole cell lysates,<br> Lane 6: human PC-3 whole cell lysates,<br> Lane 7: rat brain tissue lysates,<br> Lane 8: mouse brain tissue lysates,<br> Lane 9: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fascin/FSCN1 antigen affinity purified polyclonal antibody (Catalog # PB9592) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fascin/FSCN1 at approximately 55 kDa. The expected band size for Fascin/FSCN1 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fut1-picoband-trade-antibody-pb9593-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9593-1-WB-anti-fut1-picoband-antibody.jpgAnti-Galactoside 2-alpha-L-fucosyltransferase 1/FUT1 Antibody Picoband&reg; Western blot analysis of FUT1 using anti-FUT1 antibody (PB9593). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Kidney Tissue Lysate at 50ug,<br> Lane 2: SW620 Whole Cell Lysate at 40ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUT1 antigen affinity purified polyclonal antibody (Catalog # PB9593) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FUT1 at approximately 50 kDa. The expected band size for FUT1 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9593-2-IHC-anti-fut1-picoband-antibody.jpgAnti-Galactoside 2-alpha-L-fucosyltransferase 1/FUT1 Antibody Picoband&reg; IHC analysis of FUT1 using anti-FUT1 antibody (PB9593). <br> FUT1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FUT1 Antibody (PB9593) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9593-3-IHC-anti-fut1-picoband-antibody.jpgAnti-Galactoside 2-alpha-L-fucosyltransferase 1/FUT1 Antibody Picoband&reg; IHC analysis of FUT1 using anti-FUT1 antibody (PB9593). <br> FUT1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FUT1 Antibody (PB9593) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9593-4-IHC-anti-fut1-picoband-antibody.jpgAnti-Galactoside 2-alpha-L-fucosyltransferase 1/FUT1 Antibody Picoband&reg; IHC analysis of FUT1 using anti-FUT1 antibody (PB9593). <br> FUT1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FUT1 Antibody (PB9593) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-frizzled-homolog-1-picoband-trade-antibody-pb9594-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9594-1-WB-anti-frizzled-homolog-1-picoband-antibody.jpgAnti-Frizzled homolog 1/FZD1 Antibody Picoband&reg; Western blot analysis of Frizzled homolog 1 using anti-Frizzled homolog 1 antibody (PB9594). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: 22RV1 Whole Cell Lysate at 40ug,<br> Lane 2: 293T Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Frizzled homolog 1 antigen affinity purified polyclonal antibody (Catalog # PB9594) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Frizzled homolog 1 at approximately 71 kDa. The expected band size for Frizzled homolog 1 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9594-2-IHC-anti-frizzled-homolog-1-picoband-antibody.jpgAnti-Frizzled homolog 1/FZD1 Antibody Picoband&reg; IHC analysis of Frizzled homolog 1 using anti-Frizzled homolog 1 antibody (PB9594). <br> Frizzled homolog 1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Frizzled homolog 1 Antibody (PB9594) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gabrb3-picoband-trade-antibody-pb9595-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9595-1-WB-anti-gabrb3-gaba-a-receptor-beta-3-picoband-antibody.jpgAnti-GABA A Receptor beta 3/GABRB3 Antibody Picoband&reg; Western blot analysis of GABRB3 using anti-GABRB3 antibody (PB9595). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRB3 antigen affinity purified polyclonal antibody (Catalog # PB9595) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRB3 at approximately 54 kDa. The expected band size for GABRB3 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gja3-picoband-trade-antibody-pb9596-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9596-1-WB-anti-gja3-connexin-46-picoband-antibody.jpgAnti-GJA3 Antibody Picoband&reg; Western blot analysis of GJA3 using anti-GJA3 antibody (PB9596). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 2: Rat Kidney Tissue Lysate at 50ug,<br> Lane 3: Human Placenta Tissue Lysate at 50ug,<br> Lane 4: NIH3T3 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA3 antigen affinity purified polyclonal antibody (Catalog # PB9596) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA3 at approximately 55 kDa. The expected band size for GJA3 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hnf1-beta-picoband-trade-antibody-pb9597-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9597-1-WB-anti-hnf1-beta-picoband-antibody.jpgAnti-HNF1 beta/HNF1B Antibody Picoband&reg; Western blot analysis of HNF1 beta using anti-HNF1 beta antibody (PB9597). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Rat Kidney Tissue Lysate at 50ug,<br> Lane 3: SW620 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HNF1 beta antigen affinity purified polyclonal antibody (Catalog # PB9597) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HNF1 beta at approximately 61 kDa. The expected band size for HNF1 beta is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hspb2-picoband-trade-antibody-pb9598-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9598-1-WB-anti-hspb2-picoband-antibody.jpgAnti-HSPB2 Antibody Picoband&reg; Western blot analysis of HSPB2 using anti-HSPB2 antibody (PB9598). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB2 antigen affinity purified polyclonal antibody (Catalog # PB9598) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB2 at approximately 20 kDa. The expected band size for HSPB2 is at 20 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-5ht2a-receptor-picoband-trade-antibody-pb9599-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9599-1-WB-anti-5ht2a-receptor-picoband-antibody.jpgAnti-5HT2A Receptor/HTR2A Antibody Picoband&reg; Western blot analysis of 5HT2A Receptor using anti-5HT2A Receptor antibody (PB9599). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44;<br> Lane 2: Rat Testis Tissue Lysate&#44;<br> Lane 3: U87 Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2A Receptor antigen affinity purified polyclonal antibody (Catalog # PB9599) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2A Receptor at approximately 53KD. The expected band size for 5HT2A Receptor is at 53KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9599-htr2a-primary-antibodies-ihc-testing-4.jpgAnti-5HT2A Receptor/HTR2A Antibody Picoband&reg;IHC analysis of HTR2A using anti-HTR2A antibody (PB9599). <br>HTR2A was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HTR2A Antibody (PB9599) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9599-2.jpgAnti-5HT2A Receptor/HTR2A Antibody Picoband&reg; IHC analysis of 5HT2A Receptor using anti-5HT2A Receptor antibody (PB9599).<br> 5HT2A Receptor was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-5HT2A Receptor Antibody (PB9599) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9599-3.jpgAnti-5HT2A Receptor/HTR2A Antibody Picoband&reg; IHC analysis of 5HT2A Receptor using anti-5HT2A Receptor antibody (PB9599).<br> 5HT2A Receptor was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-5HT2A Receptor Antibody (PB9599) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-5ht2b-receptor-picoband-trade-antibody-pb9600-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9600-1-WB-anti-5ht2b-receptor-picoband-antibody.jpgAnti-5HT2B Receptor/HTR2B Antibody Picoband&reg; Western blot analysis of 5HT2B Receptor using anti-5HT2B Receptor antibody (PB9600). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: U87 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-5HT2B Receptor antigen affinity purified polyclonal antibody (Catalog # PB9600) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for 5HT2B Receptor at approximately 54 kDa. The expected band size for 5HT2B Receptor is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-idh1-picoband-trade-antibody-pb9601-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9601-idh1-primary-antibodies-wb-testing-1.jpgAnti-Isocitrate dehydrogenase/IDH1 Antibody Picoband&reg; Western blot analysis of IDH1 using anti-IDH1 antibody (PB9601). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human RT4 whole cell lysates,<br> Lane 5: human K562 whole cell lysates,<br> Lane 6: human CACO-2 whole cell lysates,<br> Lane 7: human SiHa whole cell lysates,<br> Lane 8: human A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH1 antigen affinity purified polyclonal antibody (Catalog # PB9601) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IDH1 at approximately 47 kDa. The expected band size for IDH1 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9601-2-IHC-anti-idh1-picoband-antibody.jpgAnti-Isocitrate dehydrogenase/IDH1 Antibody Picoband&reg; IHC analysis of IDH1 using anti-IDH1 antibody (PB9601).<br> IDH1 was detected in paraffin-embedded section of Mouse Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IDH1 Antibody (PB9601) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9601-3-IHC-anti-idh1-picoband-antibody.jpgAnti-Isocitrate dehydrogenase/IDH1 Antibody Picoband&reg; IHC analysis of IDH1 using anti-IDH1 antibody (PB9601).<br> IDH1 was detected in paraffin-embedded section of Rat Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IDH1 Antibody (PB9601) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9601-4-IHC-anti-idh1-picoband-antibody.jpgAnti-Isocitrate dehydrogenase/IDH1 Antibody Picoband&reg; IHC analysis of IDH1 using anti-IDH1 antibody (PB9601).<br> IDH1 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IDH1 Antibody (PB9601) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9601-idh1-primary-antibodies-ihc-testing-5.jpgAnti-Isocitrate dehydrogenase/IDH1 Antibody Picoband&reg; IHC analysis of IDH1 using anti-IDH1 antibody (PB9601).<br> IDH1 was detected in frozen section of rat small intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IDH1 Antibody (PB9601) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9601-6.jpgAnti-Isocitrate dehydrogenase/IDH1 Antibody Picoband&reg; IHC analysis of IDH1 using anti-IDH1 antibody (PB9601).<br> IDH1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-IDH1 Antibody (PB9601) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9601-7.pngAnti-Isocitrate dehydrogenase/IDH1 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-IDH1 antibody (PB9601). <br> Overlay histogram showing HepG2 cells stained with PB9601 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDH1 Antibody (PB9601&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-idh2-picoband-trade-antibody-pb9602-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9602-idh2-primary-antibodies-wb-testing-1_1.jpgAnti-IDH2 Antibody Picoband&reg; Western blot analysis of IDH2 using anti-IDH2 antibody (PB9602). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human THP-1 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human HEL whole cell lysates,<br> Lane 5: rat heart tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse heart tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDH2 antigen affinity purified polyclonal antibody (Catalog # PB9602) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IDH2 at approximately 45 kDa. The expected band size for IDH2 is at 45, 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9602-idh2-primary-antibodies-ihc-testing-2.jpgAnti-IDH2 Antibody Picoband&reg; IHC analysis of IDH2 using anti-IDH2 antibody (PB9602). <br> IDH2 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH2 Antibody (PB9602) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9602-idh2-primary-antibodies-ihc-testing-3.jpgAnti-IDH2 Antibody Picoband&reg; IHC analysis of IDH2 using anti-IDH2 antibody (PB9602). <br> IDH2 was detected in a paraffin-embedded section of human ovarican cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH2 Antibody (PB9602) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9602-idh2-primary-antibodies-ihc-testing-4.jpgAnti-IDH2 Antibody Picoband&reg; IHC analysis of IDH2 using anti-IDH2 antibody (PB9602). <br> IDH2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH2 Antibody (PB9602) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9602-idh2-primary-antibodies-ihc-testing-5_1.jpgAnti-IDH2 Antibody Picoband&reg; IHC analysis of IDH2 using anti-IDH2 antibody (PB9602). <br> IDH2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IDH2 Antibody (PB9602) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9602-idh2-primary-antibodies-if-testing-6.jpgAnti-IDH2 Antibody Picoband&reg; IF analysis of IDH2 using anti-IDH2 antibody (PB9602). <br> IDH2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-IDH2 Antibody (PB9602) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ido1-picoband-trade-antibody-pb9603-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9603-ido1-primary-antibodies-wb-testing-1.jpgAnti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband&reg;Western blot analysis of IDO1 using anti-IDO1 antibody (PB9603). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human PC-3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IDO1 antigen affinity purified polyclonal antibody (PB9603) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IDO1 at approximately 45 kDa. The expected band size for IDO1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9603-2-IHC-anti-ido1-ido-picoband-antibody.jpgAnti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband&reg; IHC analysis of IDO1 using anti-IDO1 antibody (PB9603). <br> IDO1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IDO1 Antibody (PB9603) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9603-4.pngAnti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-IDO1 antibody (PB9603). <br> Overlay histogram showing A431 cells stained with PB9603 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IDO1 Antibody (PB9603&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9603-3_1.jpgAnti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband&reg; IF analysis of IDO1 using anti-IDO1 antibody (PB9603). <br> IDO1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-IDO1 Antibody (PB9603) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9603-41419_2024_6571_fig5_html.pngAnti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband&reg;The heterogeneity within the myeloid cells. a 4 subclusters of myeloid cells were identified by UMAP analysis. b Marker gene expression of each cell type. c Comparison of cell count proportion of each cell type between imatinib resistant and sensitive patients. d mIHC staining of panCK (red), LYZ (green), IDO1 (magenta) and DAPI in GIST TME. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-024-06571-3'>38443340</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9603-41419_2024_6571_fig3_html.pngAnti-Indoleamine 2, 3-dioxygenase/IDO1 Antibody Picoband&reg;Transcription characteristics and heterogeneity among the six malignant cell types. a UMAP analysis showing the six cell type of malignant cells. b Colored cells by each sample. c Bar plot showing the cell count proportion of each maligant subcluster in GIST. d GO enrichment analysis of marker genes form BASP1_fib (left), DUSP1_fib (middle), IDO1_fib (right). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-024-06571-3'>38443340</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igfbp-1-picoband-trade-antibody-pb9604-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9604-1-WB-anti-igfbp-1-antibody.jpgAnti-IGFBP1 Antibody Picoband&reg; Western blot analysis of IGFBP-1 using anti-IGFBP-1 antibody (PB9604). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Kidney Tissue Lysate at 50ug,<br> Lane 2: Mouse Kidney Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP-1 antigen affinity purified polyclonal antibody (Catalog # PB9604) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP-1 at approximately 28 kDa. The expected band size for IGFBP-1 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igfbp2-picoband-trade-antibody-pb9605-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9605-1-WB-anti-igfbp2-picoband-antibody.jpgAnti-IGFBP2 Antibody Picoband&reg; Western blot analysis of IGFBP2 using anti-IGFBP2 antibody (PB9605). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Liver Tissue Lysate at 50ug,<br> Lane 3: Human Placenta Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP2 antigen affinity purified polyclonal antibody (Catalog # PB9605) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP2 at approximately 35 kDa. The expected band size for IGFBP2 is at 35 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-1-beta-picoband-trade-antibody-pb9606-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/o/boster-box.pngAnti-IL1 beta/IL1B AntibodyBoster Kit Box https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kin-picoband-trade-antibody-pb9607-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9607-kin-primary-antibodies-wb-testing-1.jpgAnti-KIN Antibody Picoband&reg; Western blot analysis of KIN using anti-KIN antibody (PB9607). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat RH35 whole cell lysates,<br> Lane 6: rat NRK whole cell lysates,<br> Lane 7: mouse HEPA1-6 whole cell lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIN antigen affinity purified polyclonal antibody (Catalog # PB9607) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIN at approximately 45 kDa. The expected band size for KIN is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9607-2-IHC-anti-kin-kin17-picoband-antibody.jpgAnti-KIN Antibody Picoband&reg; IHC analysis of KIN using anti-KIN antibody (PB9607). <br> KIN was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KIN Antibody (PB9607) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lipocalin-2-ngal-picoband-trade-antibody-pb9608-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9608-lcn2-primary-antibodies-wb-testing-1.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; Western blot analysis of Lipocalin-2/NGAL/LCN2 using anti-Lipocalin-2/NGAL/LCN2 antibody (PB9608). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin-2/NGAL/LCN2 antigen affinity purified polyclonal antibody (PB9608) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin-2/NGAL/LCN2 at approximately 22 kDa. The expected band size for Lipocalin-2/NGAL/LCN2 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9608-2-IHC-anti-lipocalin-2-ngal-antibody.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).<br> Lipocalin 2 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9608-3-IHC-anti-lipocalin-2-ngal-antibody.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).<br> Lipocalin 2 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9608-lipocalin_2-primary-antibodies-ihc-testing-4.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).<br> Lipocalin 2 was detected in frozen section of mouse lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9608-lipocalin_2-primary-antibodies-ihc-testing-5.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).<br> Lipocalin 2 was detected in frozen section of mouse spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9608-lipocalin_2-primary-antibodies-ihc-testing-6.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).<br> Lipocalin 2 was detected in frozen section of rat lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9608-lipocalin_2-primary-antibodies-ihc-testing-7.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9608).<br> Lipocalin 2 was detected in frozen section of rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9608) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lipocalin-2-ngal-picoband-trade-antibody-pb9609-boster.html2026-03-27T05:07:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9609-1-WB-anti-lipocalin-2-ngal-antibody.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Mouse Lung Tissue Lysate&#44;<br> Lane 2: Mouse Intestine Tissue Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9609) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22KD. The expected band size for Lipocalin 2 is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9609-2-IHC-anti-lipocalin-2-ngal-antibody.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Lipocalin 2 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9609-3-IHC-anti-lipocalin-2-ngal-antibody.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Lipocalin 2 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-lipocalin_2-primary-antibodies-ihc-testing-4.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Lipocalin 2 was detected in frozen section of mouse lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-lipocalin_2-primary-antibodies-ihc-testing-5.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Lipocalin 2 was detected in frozen section of mouse spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-lipocalin_2-primary-antibodies-ihc-testing-6.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Lipocalin 2 was detected in frozen section of rat lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-lipocalin_2-primary-antibodies-ihc-testing-7.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Lipocalin 2 was detected in frozen section of rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-lipocalin_2-primary-antibodies-ihc-testing-7_1.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg;IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9609).<br> Lipocalin 2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lipocalin 2 Antibody (PB9609) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-13578_2021_734_fig3_html.pngAnti-Lipocalin 2/LCN2 Antibody Picoband&reg;Effects of Nrf2 on MCTR1-regulated ferroptosis in CLP-induced AKI. Mice were given ML385 (30 mg/kg, ip, qd) for 7 d. On day 7, ML385 injection with or without a twice-daily administration mode of MCTR1 as described before in the following experiment. All kidney samples were collected at 24 h after CLP. A–D Shown are representative western blotting and quantification of Nrf2, GPX4, and PTGS2. E – G Quantitative analyses of MDA, GSH, and non-heme iron. H Quantitative analyses of Perl’s stain. I Representative Perl’s stain images. J Representative TEM images. The black arrow indicates ferroptosis-like mitochondria. K Quantitative analyses of mitochondrial length. L Histological analyses of renal tubular injury. M , N Representative HE stains and PAS stain. O Representative IHC images for NGAL. P Quantitative analyses of IHC stain of NGAL. Q , R Quantitative analyses of serum Scr and BUN. n = 6 mice/group, mean ± SD were presented. **P < 0.01 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-13578_2021_734_fig2_html.pngAnti-Lipocalin 2/LCN2 Antibody Picoband&reg;Effects of MCTR1 on ferroptosis in CLP-induced AKI. Mice were given MCTR1 once, twice, or not during the 24 h experiment. The once-daily administration mode (qd): Mice were given MCTR1 (200 ng/mice, iv) 0.5 h before being subjected to CLP. The twice-daily administration mode (bid): Mice were given MCTR1 (200 ng/mice, iv) 0.5 h before being subjected to CLP, and then an additional MCTR1 (200 ng/mice, iv) was given 12 h after CLP. All kidney samples were collected at 24 h after CLP. A , B Shown are representative western blotting and quantification of GPX4 and PTGS2. C–E Quantitative analyses of MDA, GSH, and non-heme iron. F Quantitative analyses of Perl’s stain. G Representative Perl’s stain images. H Representative TEM images. The black arrow indicates ferroptosis-like mitochondria. I Quantitative analyses of mitochondrial length. J Histological analyses of renal tubular injury. K , L Shown are representative HE stains and PAS stain. M Representative IHC images for NGAL. N Quantitative analyses of IHC stain of NGAL. O , P Quantitative analyses of serum Scr and BUN. n = 6 mice/group, mean ± SD were presented. **P < 0.01 . iv: intravenous <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9609-13578_2021_734_fig1_html.pngAnti-Lipocalin 2/LCN2 Antibody Picoband&reg;Ferroptosis is present in CLP-induced AKI. Mice were subjected to CLP, then the kidneys were collected at the indicated time. A , B Shown are representative western blotting and quantification of GPX4 and PTGS2. C–E Quantitative analyses of MDA, GSH, and non-heme iron. F Quantitative analyses of Perl’s stain. G Representative Perl’s stain images. H Representative transmission electron microscopy (TEM) images. The black arrow indicates ferroptosis-like mitochondria. I Quantitative analyses of mitochondrial length. Mice were pretreated with liproxstatin-1 (10 mg/kg, ip) or DFO (20 mg/kg, ip) 0.5 h before being subjected to CLP. All kidney samples were collected at 24 h after CLP. J–K Shown are representative Hematoxylin-eosin (HE) stains and Periodic Acid-Schiff (PAS) stain. L Histological analyses of renal tubular injury. M Quantitative analyses of IHC stain of NGAL. N Representative immunohistochemistry (IHC) images for NGAL. O , P Quantitative analyses of Scr and BUN. n = 6 mice/group, mean ± SD were presented. *P < 0.05, **P < 0.01 and ns : P > 0.05 . ip: intraperitoneal <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13578-021-00734-x'>34961563</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lipocalin-2-ngal-picoband-trade-antibody-pb9610-boster.html2026-03-24T05:05:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9610-1-WB-anti-lipocalin-2-ngal-antibody.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9610). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: A549 Whole Cell Lysate at 40ug,<br> Lane 2: SMMC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody (Catalog # PB9610) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22 kDa. The expected band size for Lipocalin 2 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9610-2-IHC-anti-lipocalin-2-ngal-antibody.jpgAnti-Lipocalin 2/LCN2 Antibody Picoband&reg; IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody (PB9610). <br> Lipocalin 2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lipocalin 2 Antibody (PB9610) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lamin-b1-picoband-trade-antibody-pb9611-boster.html2026-03-27T05:07:02+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-fphar-08-00950-g005.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;Effects of Klotho overexpression on neuroinflammatory responses in the ischemic brain induced by 2VO in mice. Four weeks after the intracerebroventricular injection of a lentivirus that encoded Klotho (LV-KL) or GFP (LV-GFP), the mice were exposed to 20 min 2VO and 72 h reperfusion. Western blot and qPCR were performed 72 h after surgery. (A) Representative Western blots and quantitative analysis of the expression of RIG-1 and the nuclear translocation of NF-κB. (B) The amount of RIG-I expression and NF-κB that translocated to the nucleus was normalized to β-actin or Lamin B, respectively. The results were expressed as each normalized value relative to LV-GFP-treated sham controls. (C) The mRNA levels of TNF-α and IL-6 in the brain. The results were normalized to the corresponding reporter gene GAPDH and are presented as a fold change relative to the sham-operated group. The data are expressed as mean ± SEM. One-way ANOVA followed by Dunnett’s test. n = 3–4 per group. ∗ p < 0.05, ∗∗ p < 0.01, vs. LV-GFP-treated 2VO group; # p < 0.05, vs. LV-GFP-treated sham group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2017.00950/full'>29403373</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-ijbsv13p0492g009.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg;Deguelin suppressed OVA-induced NF-κB activation in lung tissues. Total protein, cytosol protein and nuclear protein were separately extracted from lung tissues 24 h after the last OVA challenge. (A) Expressions of NF-κB p65, phospho-p65, IκBα, and phosph-IκBα were analyzed by western blotting analysis. β-actin was used as an internal control. (E) Expressions of cytosol p65 and nuclear p65 were analyzed by western blotting. β-actin and Lamin B were used as internal controls. (B, C, D, F, G, H) Grey values of the indicated proteins were measured by Quantity One software. Values are shown as mean ± SEM (n = 6 for each group). DXM = dexamethasone. # P <0.05, ## P <0.01 vs. Control group; * P <0.05, ** P <0.01 vs. OVA-challenged group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5436569/&orig_host=www.ncbi.nlm.nih.gov'>28529457</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-ijbsv13p0492g010.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; Effects of deguelin on TNF-α induced NF-κB activation in BEAS-2B cells. BEAS-2B cells were pretreated with 10 μM deguelin for 24 h and then exposed to 10 ng/ml TNF-α for the indicated times. (A) Expressions of NF-κB p65, phospho-p65, IκBα, phospho-IκBα and nuclear p65 were analyzed by Western Blotting analysis. β-actin and Lamin B were used as internal controls. (B, C, D, E, F) Grey values of the indicated proteins were measured by Quantity One software. Values are shown as mean ± SEM of three independent experiments. # P <0.05, ## P <0.01 vs. Medium plus TNF-α-10 min group; * P <0.05, ** P <0.01 vs. Medium plus TNF-α-30 min group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5436569/&orig_host=www.ncbi.nlm.nih.gov'>28529457</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ip-testing-10.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; Immunoprecipitating Lamin B1 in Hela whole cell lysate. <br>Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br>Lane 1: Hela whole cell lysates (30ug), <br>Lane 2: Rabbit control IgG instead of anti-Lamin B1 antibody in Hela whole cell lysate, <br>Lane 3: anti-Lamin B1 antibody (2μg) + Hela whole cell lysate (500μg). <br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (PB9611) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Lamin B1 at approximately 66 kDa. The expected band size for Lamin B1 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-wb-testing-1.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; Western blot analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U2OS whole cell lysates, <br> Lane 2: human THP-1 whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: human RT4 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat lung tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lamin B1 antigen affinity purified polyclonal antibody (Catalog # PB9611) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lamin B1 at approximately 72 kDa. The expected band size for Lamin B1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ihc-testing-2.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ihc-testing-3.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ihc-testing-4.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ihc-testing-5.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ihc-testing-6.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in a paraffin-embedded section of human urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ihc-testing-7.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in a paraffin-embedded section of mouse cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-ihc-testing-8.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IHC analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in a paraffin-embedded section of rat cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-if-testing-9.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; IF analysis of Lamin B1 using anti-Lamin B1 antibody (PB9611). <br> Lamin B1 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Lamin B1 Antibody (PB9611) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9611-lmnb1-primary-antibodies-fcm-testing-11.jpgAnti-Lamin B1/LMNB1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-Lamin B1 antibody (PB9611). <br> Overlay histogram showing A431 cells stained with PB9611 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lamin B1 Antibody (PB9611, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mica-picoband-trade-antibody-pb9612-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9612-mica-primary-antibodies-wb-testing-1.jpgAnti-MICA Antibody Picoband&reg; Western blot analysis of MICA using anti-MICA antibody (PB9612). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates, <br> Lane 2: human SGC-7901 whole cell lysates,<br> Lane 3: human SW620 whole cell lysates,<br> Lane 4: huamn Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICA antigen affinity purified polyclonal antibody (Catalog # PB9612) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICA at approximately 45 kDa. The expected band size for MICA is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-micb-picoband-trade-antibody-pb9613-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9613-micb-primary-antibodies-wb-testing-1.jpgAnti-MICB Antibody Picoband&reg; Western blot analysis of MICB using anti-MICB antibody (PB9613). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human HEL whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: human 293T whole cell lysates,<br> Lane 6: human SiHa whole cell lysates,<br> Lane 7: human HEPA1/6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICB antigen affinity purified polyclonal antibody (Catalog # PB9613) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICB at approximately 60-65 kDa. The expected band size for MICB is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9613-micb-primary-antibodies-fcm-testing-2.jpgAnti-MICB Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-MICB antibody (PB9613). <br> Overlay histogram showing K562 cells stained with PB9613 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-MICB Antibody (PB9613, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mnat1-picoband-trade-antibody-pb9614-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9614-mnat1-primary-antibodies-wb-testing-1.jpgAnti-MNAT1 Antibody Picoband&reg; Western blot analysis of MNAT1 using anti-MNAT1 antibody (PB9614). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human A375 whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates, <br> Lane 5: rat testis tissue lysates, <br> Lane 6: rat L6 whole cell lysates, <br> Lane 7: mouse testis tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MNAT1 antigen affinity purified polyclonal antibody (Catalog # PB9614) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MNAT1 at approximately 36 kDa. The expected band size for MNAT1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9614-2-IHC-anti-mnat1-picoband-antibody.jpgAnti-MNAT1 Antibody Picoband&reg; IHC analysis of MNAT1 using anti-MNAT1 antibody (PB9615). <br> MNAT1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MNAT1 Antibody (PB9615) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9614-3-IHC-anti-mnat1-picoband-antibody.jpgAnti-MNAT1 Antibody Picoband&reg; IHC analysis of MNAT1 using anti-MNAT1 antibody (PB9615). <br> MNAT1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MNAT1 Antibody (PB9615) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9614-4-IHC-anti-mnat1-picoband-antibody.jpgAnti-MNAT1 Antibody Picoband&reg; IHC analysis of MNAT1 using anti-MNAT1 antibody (PB9615). <br> MNAT1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MNAT1 Antibody (PB9615) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9614-mnat1-primary-antibodies-if-testing-5..jpgAnti-MNAT1 Antibody Picoband&reg; IF analysis of MNAT1 using anti-MNAT1 antibody (PB9614). <br> MNAT1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MNAT1 Antibody (PB9614) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9614-mnat1-primary-antibodies-fcm-testing-6.pngAnti-MNAT1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-MNAT1 antibody (PB9614). <br>Overlay histogram showing A431 cells stained with PB9614 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MNAT1 Antibody (PB9614, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p95-nbs1-picoband-trade-antibody-pb9615-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-nbn-primary-antibodies-wb-testing-1.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; Western blot analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human U20S whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse testis tissue lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p95 NBS1 antigen affinity purified polyclonal antibody (Catalog # PB9615) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p95 NBS1 at approximately 95 kDa. The expected band size for p95 NBS1 is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-nbn-primary-antibodies-ihc-testing-2.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). <br> p95 NBS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-nbn-primary-antibodies-ihc-testing-3.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). <br> p95 NBS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-nbn-primary-antibodies-ihc-testing-4.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). <br> p95 NBS1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-nbn-primary-antibodies-ihc-testing-5.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). <br> p95 NBS1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-nbn-primary-antibodies-ihc-testing-6.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615). <br> p95 NBS1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-p95_nbs1-primary-antibodies-ihc-testing-7.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615).<br> p95 NBS1 was detected in immunocytochemical section of SW480 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-p95_nbs1-primary-antibodies-ihc-testing-8.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615).<br> p95 NBS1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-p95_nbs1-primary-antibodies-ihc-testing-9.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IHC analysis of p95 NBS1 using anti-p95 NBS1 antibody (PB9615).<br> p95 NBS1 was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-p95 NBS1 Antibody (PB9615) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9615-nbn-primary-antibodies-if-testing-10.jpgAnti-p95 NBS1/NBN Antibody Picoband&reg; IF analysis of p95 NBS1 and Tubulin beta using anti-p95 NBS1 antibody (PB9615) and anti-Tubulin beta antibody (M03989-3).<br> p95 NBS1 and Tubulin beta were detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-p95 NBS1 antibody (PB9615) and mouse anti-Tubulin beta Antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-otoferlin-picoband-trade-antibody-pb9616-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9616-otof-primary-antibodies-wb-testing-1.jpgAnti-Otoferlin Antibody Picoband&reg;Western blot analysis of OTOF using anti-OTOF antibody (PB9616). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTOF antigen affinity purified polyclonal antibody (PB9616) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OTOF at approximately 227 kDa. The expected band size for OTOF is at 227 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9616-otof-primary-antibodies-ihc-testing-1.jpgAnti-Otoferlin Antibody Picoband&reg;IHC analysis of OTOF using anti-OTOF antibody (PB9616). <br> OTOF was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OTOF Antibody (PB9616) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9616-otof-primary-antibodies-ihc-testing-2.jpgAnti-Otoferlin Antibody Picoband&reg;IHC analysis of OTOF using anti-OTOF antibody (PB9616). <br> OTOF was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-OTOF Antibody (PB9616) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-surfactant-protein-d-picoband-trade-antibody-pb9617-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9617-1-WB-anti-sp-d-antibody.jpgAnti-Surfactant protein D/SFTPD Antibody Picoband&reg; Western blot analysis of Surfactant protein D using anti-Surfactant protein D antibody (PB9617). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Lung Tissue Lysate at 50ug,<br> Lane 2: Rat Brain Tissue Lysate at 50ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Surfactant protein D antigen affinity purified polyclonal antibody (Catalog # PB9617) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Surfactant protein D at approximately 38 kDa. The expected band size for Surfactant protein D is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9617-2-IHC-anti-sp-d-antibody.jpgAnti-Surfactant protein D/SFTPD Antibody Picoband&reg; IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (PB9617). <br> Surfactant protein D was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Surfactant protein D Antibody (PB9617) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9617-surfactant_protein_d-primary-antibodies-ihc-testing-3.jpgAnti-Surfactant protein D/SFTPD Antibody Picoband&reg; IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (PB9617).<br> Surfactant protein D was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Surfactant protein D Antibody (PB9617) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9617-surfactant_protein_d-primary-antibodies-ihc-testing-4.jpgAnti-Surfactant protein D/SFTPD Antibody Picoband&reg; IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (PB9617).<br> Surfactant protein D was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-Surfactant protein D Antibody (PB9617) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9617-surfactant_protein_d-primary-antibodies-ihc-testing-5.jpgAnti-Surfactant protein D/SFTPD Antibody Picoband&reg; IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody (PB9617). <br> Surfactant protein D was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Surfactant protein D Antibody (PB9617) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sox4-picoband-trade-antibody-pb9618-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9618-1-WB-anti-sox4-picoband-antibody.jpgAnti-SOX4 Antibody Picoband&reg; Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: MCF-7 Whole Cell Lysate&#44;<br> Lane 2: 293T Whole Cell Lysate.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9618-2.jpgAnti-SOX4 Antibody Picoband&reg; Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse brain tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt3-picoband-trade-antibody-pb9619-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9619-1_1-WB-anti-wnt3-picoband-antibody.jpgAnti-Wnt3 Antibody Picoband&reg; Western blot analysis of WNT3 using anti-WNT3 antibody (PB9619). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug,<br> Lane 2: HEPG2 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT3 antigen affinity purified polyclonal antibody (Catalog # PB9619) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT3 at approximately 45 kDa. The expected band size for WNT3 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-livin-antibody-rp1081-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1081-1-WB-anti-livin-antibody.jpgAnti-Livin/BIRC7 Antibody Picoband&reg;Anti-Livin antibody&#44; RP1081&#44; Western blotting<br>All lanes: Anti Livin (RP1081) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: A431 Whole Cell Lysate at 40ug<br> Predicted bind size: 33KD<br> Observed bind size: 33KD https://www.bosterbio.com/products/primary-antibodies/anti-kat13d-clock-antibody-rp1082-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1082-clock-primary-antibodies-wb-testing-1.jpgAnti-KAT13D/CLOCK Antibody Picoband&reg; Western blot analysis of KAT13D/CLOCK using anti-KAT13D/CLOCK antibody (RP1082). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: monkey COS-7 whole cell lysates, <br> Lane 5: rat L6 whole cell lysates, <br> Lane 6: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT13D/CLOCK antigen affinity purified polyclonal antibody (RP1082) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KAT13D/CLOCK at approximately 110 kDa. The expected band size for KAT13D/CLOCK is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1082-clock-primary-antibodies-ihc-testing-2.jpgAnti-KAT13D/CLOCK Antibody Picoband&reg; IHC analysis of KAT13D/CLOCK using anti-KAT13D/CLOCK antibody (RP1082). <br> KAT13D/CLOCK was detected in a paraffin-embedded section of human pancrese cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KAT13D/CLOCK Antibody (RP1082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1082-clock-primary-antibodies-ihc-testing-3.jpgAnti-KAT13D/CLOCK Antibody Picoband&reg; IHC analysis of KAT13D/CLOCK using anti-KAT13D/CLOCK antibody (RP1082). <br> KAT13D/CLOCK was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KAT13D/CLOCK Antibody (RP1082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1082-clock-primary-antibodies-ihc-testing-4.jpgAnti-KAT13D/CLOCK Antibody Picoband&reg; IHC analysis of KAT13D/CLOCK using anti-KAT13D/CLOCK antibody (RP1082). <br> KAT13D/CLOCK was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KAT13D/CLOCK Antibody (RP1082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1082-clock-primary-antibodies-if-testing-5.jpgAnti-KAT13D/CLOCK Antibody Picoband&reg; IF analysis of KAT13D/CLOCK using anti-KAT13D/CLOCK antibody (RP1082) and anti-Tubulin Alpha antibody (M03989-3).<br> KAT13D/CLOCK was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-KAT13D/CLOCK Antibody (RP1082) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1082-clock-primary-antibodies-fcm-testing-6.jpgAnti-KAT13D/CLOCK Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-KAT13D/CLOCK antibody (RP1082). <br> Overlay histogram showing Hela cells stained with RP1082 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KAT13D/CLOCK Antibody (RP1082, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dmrt1-antibody-rp1083-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1083-dmrt1-primary-antibodies-wb-testing-1.jpgAnti-DMRT1 Antibody Picoband&reg; Western blot analysis of DMRT1 using anti-DMRT1 antibody (RP1083). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>Lane 1: rat testis tissue lysates&#44;<br>Lane 2: mouse testis tissue lysates. <br>After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DMRT1 antigen affinity purified polyclonal antibody (Catalog # RP1083) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DMRT1 at approximately 39KD. The expected band size for DMRT1 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/R/P/RP1083-DMRT1-primary-antibodies-IHC-testing-2.jpgAnti-DMRT1 Antibody Picoband&reg; IHC analysis of DMRT1 using anti-DMRT1 antibody (RP1083).<br>DMRT1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DMRT1 Antibody (RP1083) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/R/P/RP1083-DMRT1-primary-antibodies-IHC-testing-3.jpgAnti-DMRT1 Antibody Picoband&reg; IHC analysis of DMRT1 using anti-DMRT1 antibody (RP1083).<br>DMRT1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DMRT1 Antibody (RP1083) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/R/P/RP1083-DMRT1-primary-antibodies-IHC-testing-4.jpgAnti-DMRT1 Antibody Picoband&reg; IHC analysis of DMRT1 using anti-DMRT1 antibody (RP1083).<br>DMRT1 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DMRT1 Antibody (RP1083) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. <br> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fabp4-antibody-rp1085-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1085-fabp4-primary-antibodies-wb-testing-1_1.jpgAnti-FABP4 Antibody Picoband&reg; Western blot analysis of FABP4 using anti-FABP4 antibody (RP1085). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: rat heart tissue lysates,<br> Lane 4: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP4 antigen affinity purified polyclonal antibody (Catalog # RP1085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP4 at approximately 15 kDa. The expected band size for FABP4 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1085-fgene-16-1529797-g002.jpgAnti-FABP4 Antibody Picoband&reg;H19 participates in increased adipogenesis of BMSCs and positively regulates PPARγ expression in SONFH. (A) Expression level of H19 in BMSCs after transfection with sh-H19. (B) Oil Red O staining (200×) of BMSCs after transfection with sh-H19. (C, D) Quantification of Oil Red O staining (200×) of BMSCs after transfection with sh-H19. (E, F) The expression of fatty acid-binding protein 4 (FABP4) was detected by qRT-PCR and Western blot after knocking-down of H19. (G) The expression levels of H19 and PPARγ 1 week following the knockdown of H19. (n = 3, all data are shown as the mean ± SD of three independent experiments, *p < 0.05, **p < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1529797/full'>40259926</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1085-fgene-16-1529797-g003.jpgAnti-FABP4 Antibody Picoband&reg;H19 modulates PPARγ expression through miR-130b-3p. (A) Three databases, StarBase, DIANA tools, and miRWalk, were used to predict candidate micro (mi)RNAs as shown in the Venn diagram. (B) Expression levels of three candidate miRNAs (miR-301b-3p, miR-130b-3p, and miR-130a-3p) after knockdown of H19. (C) The binding sites of miR-130b-3p with H19 and PPARγ. (D) Effect of miR-130b-3p on the luciferase activity of wild-type (WT)-H19 and mutant (MT)-H19 reporter systems. (E) Effect of miR-130b-3p on the luciferase activity of WT-FABP4 and MT-FABP4 reporter systems was detected via luciferase reporter assay. (F, G) The expression of PPARγ is significantly decreased upon knocking down H19. This reduction could be reversed through co-transfection with a miR-130b-3p inhibitor. (H, I) The expression of PPARγ was significantly augmented when H19 was upregulated. This elevation in PPARγ expression could be counteracted by co-transfecting with a miR-130b-3p mimic. (n = 3, all data are shown as the mean ± SD of three independent experiments, **p < 0.01, ***p < 0.001).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/genetics/articles/10.3389/fgene.2025.1529797/full'>40259926</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1085-2-IHC-anti-fabp4-a-fabp-antibody.jpgAnti-FABP4 Antibody Picoband&reg; IHC analysis of FABP4 using anti-FABP4 antibody (RP1085). <br> FABP4 was detected in a paraffin-embedded section of Mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP4 Antibody (RP1085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1085-3-IHC-anti-fabp4-a-fabp-antibody.jpgAnti-FABP4 Antibody Picoband&reg; IHC analysis of FABP4 using anti-FABP4 antibody (RP1085). <br> FABP4 was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP4 Antibody (RP1085) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fos-b-antibody-rp1086-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1086-fosb-primary-antibodies-wb-testing-1.jpgAnti-Fos B/FOSB Antibody Picoband&reg; Western blot analysis of FOSB using anti-FOSB antibody (RP1086). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates, <br> Lane 2: mouse thymus tissue lysates, <br> Lane 3: mouse ovary tissue lysates, <br> Lane 4: mouse spleen tissue lysates, <br> Lane 5: mouse kidney tissue lysates, <br> Lane 6: mouse RAW246.7 whole cell lysates, <br> Lane 7: human HL-60 whole cell lysates, <br> Lane 8: human THP-1 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB antigen affinity purified polyclonal antibody (Catalog # RP1086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOSB at approximately 35KD. The expected band size for FOSB is at 35KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1086-fosb-primary-antibodies-ihc-testing-2.jpgAnti-Fos B/FOSB Antibody Picoband&reg; IHC analysis of FOSB using anti-FOSB antibody (RP1086). <br> FOSB was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FOSB Antibody (RP1086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1086-fosb-primary-antibodies-ihc-testing-3.jpgAnti-Fos B/FOSB Antibody Picoband&reg; IHC analysis of FOSB using anti-FOSB antibody (RP1086). <br> FOSB was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FOSB Antibody (RP1086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1086-fosb-primary-antibodies-ihc-testing-4.jpgAnti-Fos B/FOSB Antibody Picoband&reg; IHC analysis of FOSB using anti-FOSB antibody (RP1086). <br> FOSB was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FOSB Antibody (RP1086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1086-fosb-primary-antibodies-ihc-testing-5.jpgAnti-Fos B/FOSB Antibody Picoband&reg; IHC analysis of FOSB using anti-FOSB antibody (RP1086). <br> FOSB was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FOSB Antibody (RP1086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1086-fosb-primary-antibodies-fc-testing-6.pngAnti-Fos B/FOSB Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-FOSB antibody (RP1086). <br>Overlay histogram showing SiHa cells stained with RP1086 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOSB Antibody (RP1086, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ptov1-antibody-rp1087-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1087-1-WB-anti-ptov1-antibody.jpgAnti-PTOV1 Antibody Picoband&reg;Anti-PTOV1 antibody&#44; RP1087&#44; Western blotting<br>All lanes: Anti PTOV1 (RP1087) at 0.5ug/ml<br> Lane 1: Rat Brain Tissue Lysate at 50ug<br> Lane 2: Rat Kidney Tissue Lysate at 50ug<br> Predicted bind size: 47KD<br> Observed bind size: 47KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnt7a-antibody-rp1088-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1088-wnt7a-primary-antibodies-wb-testing-1.jpgAnti-Protein Wnt-7a Wnt7a Antibody Picoband&reg; Western blot analysis of Wnt7a using anti-Wnt7a antibody (RP1088). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.<br> Lane 3: rat kidney tissue lysates, <br> Lane 4: rat liver tissue lysates, <br> Lane 5: mouse kidney tissue lysates, <br> Lane 6: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt7a antigen affinity purified polyclonal antibody (Catalog # RP1088) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt7a at approximately 39 kDa. The expected band size for Wnt7a is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1088-wnt7a-primary-antibodies-fcm-testing-2.jpgAnti-Protein Wnt-7a Wnt7a Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-Wnt7a antibody (RP1088). <br> Overlay histogram showing PC-3 cells stained with RP1088 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Wnt7a Antibody (RP1088, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/picokine-elisa-kits/human-activin-a-picokine-trade-elisa-kit-ek0301-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0301.pngHuman Activin A ELISA Kit PicoKine®Human Activin A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-igfbp4-picokine-trade-elisa-kit-ek0388-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0388.jpgHuman IGFBP4 PicoKine® ELISA KitHuman IGFBP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-scf-picokine-trade-elisa-kit-ek0507-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0507_1.pngHuman SCF/KITLG/Kit ligand ELISA Kit PicoKine®Human SCF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfr1-picokine-trade-elisa-kit-ek0529-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0529_1.pngMouse TNFsR I ELISA Kit PicoKine®Mouse TNFR1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd86-b7-2-picokine-trade-elisa-kit-ek0711-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0711_1.pngMouse CD86/B7-2 ELISA Kit PicoKine®Mouse CD86/B7-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cxcl2-mip-2-picokine-trade-elisa-kit-ek0725-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0725_1.pngRat MIP-2 ELISA Kit PicoKine®Rat CXCL2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl21-6ckine-picokine-trade-elisa-kit-ek0556-boster.html2026-03-24T05:05:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0556_1.pngMouse CCL21/6Ckine/Ccl21a ELISA Kit PicoKine®Mouse CCL21/6Ckine PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dan-nbl1-picokine-trade-elisa-kit-ek0746-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0746.jpgHuman DAN/NBL1 ELISA Kit PicoKine®Human DAN/NBL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-dan-nbl1-picokine-trade-elisa-kit-ek0747-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0747_1.pngMouse DAN/NBL1 ELISA Kit PicoKine®Mouse DAN/NBL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-il-17ra-picokine-trade-elisa-kit-ek0784-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0784_1.pngHuman IL-17RA ELISA Kit PicoKine®Human IL-17RA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-aggrecan-picokine-trade-elisa-kit-ek0909-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0909.jpgHuman Aggrecan/ACAN ELISA Kit PicoKine®Human Aggrecan PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tnfrsf16-ngfr-picokine-trade-elisa-kit-ek0972-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0972_1.pngHuman TNFRSF16 / NGFR / CD271 ELISA Kit PicoKine®Human TNFRSF16/NGFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-klk11-picokine-trade-elisa-kit-ek1166-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1166_2.pngHuman KLK11/Kallikrein-11 ELISA Kit PicoKine®Human KLK11 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-klk12-picokine-trade-elisa-kit-ek1167-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1167_1.pngHuman KLK12/Klk L5 ELISA Kit PicoKine®Human KLK12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-klk13-picokine-trade-elisa-kit-ek1168-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1168_1.pngHuman KLK13/Kallikrein-13 ELISA Kit PicoKine®Human KLK13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-s100a12-picokine-trade-elisa-kit-ek1169-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1169_1.pngHuman S100A12/En Rage ELISA Kit PicoKine®Human S100A12 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tenascin-c-tnc-picokine-trade-elisa-kit-ek1170-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1170.jpgHuman Tenascin-C/TNC ELISA Kit PicoKine®Human Tenascin-C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-relaxin-1-picokine-trade-elisa-kit-ek1230-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1230.jpgHuman Relaxin 1 ELISA Kit PicoKine®Human Relaxin 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-fgf21-picokine-trade-elisa-kit-ek1379-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1379_2.pngMouse FGF21 ELISA Kit PicoKine®Mouse FGF21 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tgfbr3-picokine-trade-elisa-kit-ek1383-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1383_2.pngHuman TGFBR3/Tgf Beta Riii ELISA Kit PicoKine®Human TGFBR3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-loxl2-picokine-trade-elisa-kit-ek1391-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1391_1.pngHuman LOXL2/Lysyl Oxidase Like 2 ELISA Kit PicoKine®Human LOXL2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-serpin-c1-antithrombin-iii-picokine-trade-elisa-kit-ek1393-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1393.jpgHuman Serpin C1/Antithrombin-III ELISA Kit PicoKine®Human Serpin C1/Antithrombin-III PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-epiregulin-picokine-trade-elisa-kit-ek1394-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1394.jpgHuman Epiregulin ELISA Kit PicoKine®Human Epiregulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-epiregulin-picokine-trade-elisa-kit-ek1395-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1395.jpgMouse Epiregulin / EREG ELISA Kit PicoKine®Mouse Epiregulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-erbb3-her3-picokine-trade-elisa-kit-ek1398-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1398_1.pngHuman ERBB3/Her3 ELISA Kit PicoKine®Human ERBB3/Her3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-complement-h-cfh-picokine-trade-elisa-kit-ek1399-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1399.jpgHuman Complement Factor H/CFH ELISA Kit PicoKine®Human Complement H/CFH PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-complement-h-cfh-picokine-trade-elisa-kit-ek1400-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1400.jpgMouse Complement Factor H/CFH ELISA Kit PicoKine®Mouse Complement H/CFH PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-chordin-picokine-trade-elisa-kit-ek1401-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1401_1.pngMouse Chordin ELISA Kit PicoKine®Mouse Chordin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd97-picokine-trade-elisa-kit-ek1402-boster.html2026-03-24T05:05:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1402.jpgHuman CD97/ADGRE5 ELISA Kit PicoKine®Human CD97 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-stanniocalcin-1-stc1-picokine-trade-elisa-kit-ek1404-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1404_1.pngHuman Stanniocalcin 1 / STC1 / STC ELISA Kit PicoKine®Human Stanniocalcin 1/STC1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cripto-tdgf1-picokine-trade-elisa-kit-ek1405-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1405_1.pngMouse Cripto/TDGF1 ELISA Kit PicoKine®Mouse Cripto/TDGF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-comt-picokine-trade-elisa-kit-ek1406-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1406_1.pngHuman COMT ELISA Kit PicoKine®Human COMT PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-relaxin-3-picokine-trade-elisa-kit-ek1407-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1407_1.pngHuman Relaxin-3/RLN3 ELISA Kit PicoKine®Human Relaxin 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-nrcam-picokine-trade-elisa-kit-ek1408-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1408_1.pngHuman NRCAM ELISA Kit PicoKine®Human NRCAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cxcl5-lix-picokine-trade-elisa-kit-ek0823-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0823_1.pngRat CXCL5/LIX/ENA-78 ELISA Kit PicoKine®Rat CXCL5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adam8-picokine-trade-elisa-kit-ek0650-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0650_1.pngHuman ADAM8/Ms2 ELISA Kit PicoKine®Human ADAM8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-oncostatin-m-osm-picokine-trade-elisa-kit-ek0479-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0479_1.pngMouse OSM/Oncostatin M ELISA Kit PicoKine®Mouse Oncostatin M/OSM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dcr3-tnfrsf6b-picokine-trade-elisa-kit-ek0748-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0748.pngHuman DCR3/TNFRSF6B ELISA Kit PicoKine®Human DCR3/TNFRSF6B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl9-picokine-trade-elisa-kit-ek0733-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0733_1.pngMouse CXCL9/Mig ELISA Kit PicoKine®Mouse CXCL9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-fgf23-picokine-trade-elisa-kit-ek1362-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1362_2.pngMouse FGF23 ELISA Kit PicoKine®Mouse FGF23 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-alcam-picokine-trade-elisa-kit-ek1184-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1184.jpgMouse ALCAM/CD166 ELISA Kit PicoKine®Mouse ALCAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-beta-ig-h3-tgfbi-picokine-trade-elisa-kit-ek1191-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1191.pngMouse beta IG-H3/TGFBI ELISA Kit PicoKine®Mouse βIG-H3/TGFBI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ho-1-hmox1-picokine-trade-elisa-kit-ek0888-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0888.jpgHuman HO-1/HMOX1/HSP32 ELISA Kit PicoKine®Human HO-1/HMOX1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-s100a8-picokine-trade-elisa-kit-ek1150-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1150_2.pngMouse S100A8/Calgranulin A ELISA Kit PicoKine®Mouse S100A8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-granzyme-a-picokine-trade-elisa-kit-ek1162-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1162_1.pngHuman Granzyme A ELISA Kit PicoKine®Human Granzyme A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pedf-serpinf1-picokine-trade-elisa-kit-ek0896-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0896_6.pngHuman PEDF/SerpinF1 ELISA Kit PicoKine®Human PEDF/SerpinF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adamts13-picokine-trade-elisa-kit-ek0927-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0927.jpgHuman ADAMTS13 ELISA Kit PicoKine®Human ADAMTS13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-galectin-3bp-picokine-trade-elisa-kit-ek1240-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1240.pngHuman Galectin-3BP ELISA Kit PicoKine®Human Galectin-3BP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ve-cadherin-cd144-picokine-trade-elisa-kit-ek1317-boster.html2026-04-03T05:00:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1317_1.pngHuman VE-Cadherin CDH5 ELISA Kit PicoKine®Human VE-Cadherin/CD144 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-il-11-picokine-trade-elisa-kit-ek0420-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0420_1.pngMouse IL-11/Interleukin-11 ELISA Kit PicoKine®Mouse IL-11 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cathepsin-l-picokine-trade-elisa-kit-ek0676-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0676_1.pngHuman Cathepsin L ELISA Kit PicoKine®Human Cathepsin L PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cxcl3-picokine-trade-elisa-kit-ek1364-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1364_1.pngMouse CXCL3 / GRO gamma ELISA Kit PicoKine®Mouse CXCL3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd30-tnfrsf8-picokine-trade-elisa-kit-ek0569-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0569.pngHuman CD30/TNFRSF8 ELISA Kit PicoKine®Human CD30/TNFRSF8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-klk8-kallikrein-8-picokine-trade-elisa-kit-ek0819-boster.html2026-03-24T05:05:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0819_1.pngHuman KLK8/Kallikrein-8 ELISA Kit PicoKine®Human KLK8/Kallikrein-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-csf3r-g-csf-r-picokine-trade-elisa-kit-ek0766-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0766_1.pngHuman CSF3R/G-CSF R ELISA Kit PicoKine®Human CSF3R/G-CSF R PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-xcl1-lymphotactin-picokine-trade-elisa-kit-ek0803-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0803_2.pngMouse XCL1 / Lymphotactin / SCM 1 ELISA Kit PicoKine®Mouse XCL1/Lymphotactin PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cofilin-picoband-trade-antibody-pb9620-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9620-cofilin-primary-antibodies-wb-testing-1.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; Western blot analysis of Cofilin using anti-Cofilin antibody (PB9620). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human U-87 MG whole cell lysates,<br> Lane 4: monkey COS-7 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin antigen affinity purified polyclonal antibody (Catalog # PB9620) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin at approximately 19 kDa. The expected band size for Cofilin is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9620-2-IHC-anti-cofilin-picoband-antibody.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9620). <br> Cofilin was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cofilin Antibody (PB9620) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9620-3-IHC-anti-cofilin-picoband-antibody.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9620). <br> Cofilin was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cofilin Antibody (PB9620) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9620-4-IHC-anti-cofilin-picoband-antibody.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IHC analysis of Cofilin using anti-Cofilin antibody (PB9620). <br> Cofilin was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cofilin Antibody (PB9620) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9620-cofilin-primary-antibodies-if-testing-5.jpgAnti-Cofilin/CFL1 Antibody Picoband&reg; IF analysis of Cofilin using anti-Cofilin antibody (PB9620). <br> Cofilin was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cofilin Antibody (PB9620) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il-8-picoband-trade-antibody-pb9621-boster.html2026-03-16T09:15:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9621-il-8-primary-antibodies-wb-testing-1.jpgAnti-IL8/CXCL8 Antibody Picoband&reg; Western blot analysis of IL-8 using anti-IL-8 antibody (PB9621). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: wild-type Hela whole cell lysate,<br> Lane 2: Hela treated with TPA (400 ng/mL) for 4h whole cell lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-8 antigen affinity purified polyclonal antibody (Catalog # PB9621) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-8 at approximately 11 kDa. The expected band size for IL-8 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9621-nrr-12-1632-g003.jpgAnti-IL8/CXCL8 Antibody Picoband&reg;Effect of glycogen synthase kinase 3 beta (GSK-3β) on Bax, Bcl-2, interleukin (IL)-6, IL-8, and IL-10 immunoreactivity in the brain of diabetic rats with myocardial ischemia/reperfusion injury (%). (A–E) Representative images (original magnification, 400×) and quantification of Bax, Bcl-2, IL-6, IL-8, and IL-10 immunoreactivities. Arrows show Bax-, Bcl-2-, IL-6-, IL-8-, and IL-10-immunoreactive cells. ** P < 0.01, vs . NS group; ## P < 0.01, vs . NIR group; $$ P < 0.01, vs . NIPost group; && P < 0.01, vs . NIPostI group; §§ P < 0.01, vs . DS group; †† P < 0.01, vs . DIR group; ££ P < 0.01, vs . DIPost group (mean ± SD, n = 4, one-way analysis of variance and the Student-Newman-Keuls test). The results for the NIPostI group resembled those for the NIR group. Histopathological changes were more severe in diabetic vs . non-diabetic rats. Only the NS and DS groups received a thoracotomy. Myocardial ischemia reperfusion was conducted in the NIR and DIR groups by blocking the left anterior descending coronary artery. The NIPost and DIPost groups were subjected to three cycles of 10-second reperfusion followed by a 10-second ischemia treatment immediately at the onset of reperfusion. The NIPostI and DIPostI groups were intraperitoneally injected with GSK-3β inhibitor 10 minutes before receiving MIRI, and received three cycles of 10-second reperfusion followed by a 10-second ischemia treatment immediately at the onset of reperfusion. NS group: Normal sham group; NIR group: normal myocardial ischemia reperfusion group; NIPost group: normal ischemic post-conditioning group; NIPostI group: normal ischemic post-conditioning + GSK-3β inhibitor group; DS group: diabetic sham group; DIR group: diabetic myocardial ischemia reperfusion group; DIPost group: diabetic ischemic post-conditioning group; DIPostI group: diabetic ischemic post-conditioning + GSK-3β inhibitor group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5696844/&orig_host=www.ncbi.nlm.nih.gov'>29171428</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9621-2.jpgAnti-IL8/CXCL8 Antibody Picoband&reg; IHC analysis of IL-8 using anti-IL-8 antibody (PB9621). <br> IL-8 was detected in paraffin-embedded section of human appendicitis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IL-8 Antibody (PB9621) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ervw-1-picoband-trade-antibody-pb9622-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9622-ervw-1-primary-antibodies-wb-testing-1.jpgAnti-ERVW-1 Antibody Picoband&reg; Western blot analysis of ERVW-1 using anti-ERVW-1 antibody (PB9622). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human SH-SY5Y whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERVW-1 antigen affinity purified polyclonal antibody (Catalog # PB9622) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERVW-1 at approximately 80 kDa. The expected band size for ERVW-1 is at 80 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-flotillin-2-picoband-trade-antibody-pb9623-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9623-flotillin_2-primary-antibodies-wb-testing-1_1.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; Western blot analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: rat lung tissue lysates, <br> Lane 5: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flotillin 2 antigen affinity purified polyclonal antibody (Catalog # PB9623) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for Flotillin 2 at approximately 47 kDa. The expected band size for Flotillin 2 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9623-2-IHC-anti-flotillin-2-picoband-antibody.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Flotillin 2 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9623-3-IHC-anti-flotillin-2-picoband-antibody.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Flotillin 2 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9623-4-IHC-anti-flotillin-2-picoband-antibody.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Flotillin 2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9623-flotillin_2-primary-antibodies-ihc-testing-5.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Flotillin 2 was detected in frozen section of mouse kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9623-flotillin_2-primary-antibodies-ihc-testing-6.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Flotillin 2 was detected in frozen section of rat kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9623-flotillin_2-primary-antibodies-ihc-testing-7.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Flotillin 2 was detected in frozen section of rat small intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9623-flotillin_2-primary-antibodies-if-testing-8.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; IF analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). <br> Flotillin 2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9623-flotillin_2-primary-antibodies-fcm-testing-9.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; Flow Cytometry analysis of RH35 cells using anti-Flotillin 2 antibody (PB9623).<br>Overlay histogram showing RH35 cells stained with PB9623 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Flotillin 2 Antibody (PB9623,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9623-flotillin_2-primary-antibodies-fcm-testing-10.jpgAnti-Flotillin 2/FLOT2 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Flotillin 2 antibody (PB9623).<br>Overlay histogram showing U20S cells stained with PB9623 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Flotillin 2 Antibody (PB9623,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-connexin-45-gja7-picoband-trade-antibody-pb9624-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9624-1_1-WB-anti-connexin-45-gja7-picoband-antibody.jpgAnti-Connexin 45/GJA7/GJC1 Antibody Picoband&reg; Western blot analysis of Connexin 45/GJA7 using anti-Connexin 45/GJA7 antibody (PB9624). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Gaster Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 45/GJA7 antigen affinity purified polyclonal antibody (Catalog # PB9624) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 45/GJA7 at approximately 45 kDa. The expected band size for Connexin 45/GJA7 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gremlin-1-picoband-trade-antibody-pb9626-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9626-grem1-primary-antibodies-wb-testing-1.jpgAnti-Gremlin 1/GREM1 Antibody Picoband&reg; Western blot analysis of GREM1 using anti-GREM1 antibody (PB9626). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: rat testis tissue lysates,<br> Lane 3: mouse testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GREM1 antigen affinity purified polyclonal antibody (Catalog # PB9626) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GREM1 at approximately 21 kDa. The expected band size for GREM1 is at 21 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gsta1-a2-a3-a4-a5-picoband-trade-antibody-pb9627-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9627-gsta1-a2-a3-a4-a5-primary-antibodies-wb-testing-1.jpgAnti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody Picoband&reg; Western blot analysis of GSTA1/A2/A3/A4/A5 using anti-GSTA1/A2/A3/A4/A5 antibody (PB9627). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HCCT tissue lysates, <br> Lane 2: human HCCP tissue lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: rat RH35 whole cell lysates, <br> Lane 5: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTA1/A2/A3/A4/A5 antigen affinity purified polyclonal antibody (Catalog # PB9627) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTA1/A2/A3/A4/A5 at approximately 26 kDa. The expected band size for GSTA1/A2/A3/A4/A5 is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9627-2.jpgAnti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody Picoband&reg; IF analysis of GSTA1/A2/A3/A4/A5 using anti-GSTA1/A2/A3/A4/A5 antibody (PB9627) <br> GSTA1/A2/A3/A4/A5 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-GSTA1/A2/A3/A4/A5 Antibody (PB9627) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9627-3.jpgAnti-GSTA1/GSTA2/GSTA3/GSTA4/GSTA5 Antibody Picoband&reg; IF analysis of GSTA1/A2/A3/A4/A5 using anti-GSTA1/A2/A3/A4/A5 antibody (PB9627) <br> GSTA1/A2/A3/A4/A5 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-GSTA1/A2/A3/A4/A5 Antibody (PB9627) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdac6-picoband-trade-antibody-pb9628-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9628-hdac6-primary-antibodies-wb-testing-1.jpgAnti-HDAC6 Antibody Picoband&reg; Western blot analysis of HDAC6 using anti-HDAC6 antibody (PB9628). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human SH-SY5Y whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC6 antigen affinity purified polyclonal antibody (Catalog # PB9628) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC6 at approximately 160 kDa. The expected band size for HDAC6 is at 131 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9628-hdac6-primary-antibodies-ihc-testing-1.jpgAnti-HDAC6 Antibody Picoband&reg;IHC analysis of HDAC6 using anti-HDAC6 antibody (PB9628). <br> HDAC6 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC6 Antibody (PB9628) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9628-hdac6-primary-antibodies-ihc-testing-2.jpgAnti-HDAC6 Antibody Picoband&reg;IHC analysis of HDAC6 using anti-HDAC6 antibody (PB9628). <br> HDAC6 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC6 Antibody (PB9628) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9628-hdac6-primary-antibodies-fcm-testing-2.jpgAnti-HDAC6 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-HDAC6 antibody (PB9628). <br> Overlay histogram showing K562 cells stained with PB9628 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC6 Antibody (PB9628, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdac7-picoband-trade-antibody-pb9629-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9629-1-WB-anti-hdac7-histone-deacetylase-7-picoband-antibody.jpgAnti-HDAC7 Antibody Picoband&reg; Western blot analysis of HDAC7 using anti-HDAC7 antibody (PB9629). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Spleen Tissue Lysate at 50ug,<br> Lane 2: Rat Thymus Tissue Lysate at 50ug,<br> Lane 3: Human Placenta Tissue Lysate at 50ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC7 antigen affinity purified polyclonal antibody (Catalog # PB9629) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC7 at approximately 103 kDa. The expected band size for HDAC7 is at 103 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hdac11-picoband-trade-antibody-pb9630-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9630-hdac11-primary-antibodies-wb-testing-1.jpgAnti-HDAC11 Antibody Picoband&reg; Western blot analysis of HDAC11 using anti-HDAC11 antibody (PB9630). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat heart tissue lysates,<br> Lane 4: mouse brain tissue lysates,<br> Lane 5: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC11 antigen affinity purified polyclonal antibody (Catalog # PB9630) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC11 at approximately 37 kDa. The expected band size for HDAC11 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9630-hdac11-primary-antibodies-ihc-testing-4.jpgAnti-HDAC11 Antibody Picoband&reg;IHC analysis of HDAC11 using anti-HDAC11 antibody (PB9630). <br>HDAC11 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HDAC11 Antibody (PB9630) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9630-2-IHC-anti-hdac11-histone-deacetylase-11-picoband-antibody.jpgAnti-HDAC11 Antibody Picoband&reg; IHC analysis of HDAC11 using anti-HDAC11 antibody (PB9630). <br> HDAC11 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HDAC11 Antibody (PB9630) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9630-3-IHC-anti-hdac11-histone-deacetylase-11-picoband-antibody.jpgAnti-HDAC11 Antibody Picoband&reg; IHC analysis of HDAC11 using anti-HDAC11 antibody (PB9630). <br> HDAC11 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HDAC11 Antibody (PB9630) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hex-picoband-trade-antibody-pb9631-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9631-hex-primary-antibodies-wb-testing-1.jpgAnti-Hex/HHEX Antibody Picoband&reg; Western blot analysis of Hex using anti-Hex antibody (PB9631). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hex antigen affinity purified polyclonal antibody (Catalog # PB9631) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hex at approximately 40 kDa. The expected band size for Hex is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hint1-picoband-trade-antibody-pb9632-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9632-hint1-primary-antibodies-wb-testing-1.jpgAnti-HINT1 Antibody Picoband&reg; Western blot analysis of HINT1 using anti-HINT1 antibody (PB9632). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human Raji whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat thymus tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HINT1 antigen affinity purified polyclonal antibody (Catalog # PB9632) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HINT1 at approximately 14 kDa. The expected band size for HINT1 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9632-2-IHC-anti-hint1-picoband-antibody.jpgAnti-HINT1 Antibody Picoband&reg; IHC analysis of HINT1 using anti-HINT1 antibody (PB9632). <br> HINT1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HINT1 Antibody (PB9632) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9632-3-IHC-anti-hint1-picoband-antibody.jpgAnti-HINT1 Antibody Picoband&reg; IHC analysis of HINT1 using anti-HINT1 antibody (PB9632). <br> HINT1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HINT1 Antibody (PB9632) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9632-4-IHC-anti-hint1-picoband-antibody.jpgAnti-HINT1 Antibody Picoband&reg; IHC analysis of HINT1 using anti-HINT1 antibody (PB9632). <br> HINT1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HINT1 Antibody (PB9632) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9632-5.jpgAnti-HINT1 Antibody Picoband&reg; IF analysis of HINT1 using anti-HINT1 antibody (PB9632). <br> HINT1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HINT1 Antibody (PB9632) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9632-6_1.jpgAnti-HINT1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-HINT1 antibody (PB9632).<br> Overlay histogram showing A549 cells stained with PB9632 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HINT1 Antibody (PB9632,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmg4-picoband-trade-antibody-pb9633-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9633-1-WB-anti-hmg4-picoband-antibody.jpgAnti-HMG4/HMGB3 Antibody Picoband&reg; Western blot analysis of HMG4 using anti-HMG4 antibody (PB9633). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Mouse Liver Tissue Lysate at 50ug,<br> Lane 2: Mouse Kidney Tissue Lysate at 50ug,<br> Lane 3: Mouse Testis Tissue Lysate at 50ug,<br> Lane 4: 22RV1 Whole Cell Lysate at 40ug,<br> Lane 5: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 6: NIH3T3 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMG4 antigen affinity purified polyclonal antibody (Catalog # PB9633) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMG4 at approximately 23 kDa. The expected band size for HMG4 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9633-2-IHC-anti-hmg4-picoband-antibody.jpgAnti-HMG4/HMGB3 Antibody Picoband&reg; IHC analysis of HMG4 using anti-HMG4 antibody (PB9633). <br> HMG4 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HMG4 Antibody (PB9633) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9633-3-IHC-anti-hmg4-picoband-antibody.jpgAnti-HMG4/HMGB3 Antibody Picoband&reg; IHC analysis of HMG4 using anti-HMG4 antibody (PB9633). <br> HMG4 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HMG4 Antibody (PB9633) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9633-4-IHC-anti-hmg4-picoband-antibody.jpgAnti-HMG4/HMGB3 Antibody Picoband&reg; IHC analysis of HMG4 using anti-HMG4 antibody (PB9633). <br> HMG4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HMG4 Antibody (PB9633) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9633-hmg4-primary-antibody-if-testing-5.jpgAnti-HMG4/HMGB3 Antibody Picoband&reg; IF analysis of HMG4 using anti-HMG4 antibody (PB9633). <br> HMG4 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-HMG4 Antibody (PB9633) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9633-hmg4-primary-antibody-fcm-testing-6.pngAnti-HMG4/HMGB3 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-HMG4 antibody (PB9633). <br>Overlay histogram showing HL-60 cells stained with PB9633 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMG4 Antibody (PB9633, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsf4-picoband-trade-antibody-pb9634-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9634-1-WB-anti-hsf4-picoband-antibody.jpgAnti-HSF4 Antibody Picoband&reg; Western blot analysis of HSF4 using anti-HSF4 antibody (PB9634). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug,<br> Lane 2: MCF-7 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF4 antigen affinity purified polyclonal antibody (Catalog # PB9634) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF4 at approximately 53 kDa. The expected band size for HSF4 is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp90-alpha-picoband-trade-antibody-pb9635-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9635-hsp90aa1-primary-antibodies-wb-testing-1.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; Western blot analysis of Hsp90 alpha using anti-Hsp90 alpha antibody (PB9635). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysate,<br> Lane 2: human HEK293 whole cell lysate,<br> Lane 3: monkey COS-7 whole cell lysate,<br> Lane 4: human HepG2 whole cell lysate,<br> Lane 5: human A549 whole cell lysate,<br> Lane 6: rat PC-12 whole cell lysate,<br> Lane 7: rat RH35 whole cell lysate,<br> Lane 8: mouse HEPA1-6 whole cell lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp90 alpha antigen affinity purified polyclonal antibody (Catalog # PB9635) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp90 alpha at approximately 90 kDa. The expected band size for Hsp90 alpha is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9635-2-IHC-anti-hsp90-alpha-picoband-antibody.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; IHC analysis of Hsp90 alpha using anti-Hsp90 alpha antibody (PB9635). <br> Hsp90 alpha was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp90 alpha Antibody (PB9635) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9635-3-IHC-anti-hsp90-alpha-picoband-antibody.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; IHC analysis of Hsp90 alpha using anti-Hsp90 alpha antibody (PB9635). <br> Hsp90 alpha was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp90 alpha Antibody (PB9635) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9635-4-IHC-anti-hsp90-alpha-picoband-antibody.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; IHC analysis of Hsp90 alpha using anti-Hsp90 alpha antibody (PB9635). <br> Hsp90 alpha was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp90 alpha Antibody (PB9635) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9635-5.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; IF analysis of Hsp90 alpha using anti-Hsp90 alpha antibody (PB9635). <br> Hsp90 alpha was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hsp90 alpha Antibody (PB9635) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9635-6.jpgAnti-Hsp90 alpha/HSP90AA1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-Hsp90 alpha antibody (PB9635).<br>Overlay histogram showing SiHa cells stained with PB9635 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp90 alpha Antibody (PB9635,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp90-beta-picoband-trade-antibody-pb9636-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9636-hsp90ab1-primary-antibodies-wb-testing-1.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; Western blot analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: human HEK293 whole cell lysates,<br> Lane 6: rat PC-12 whole cell lysates, <br> Lane 7: mouse NIH/3T3 whole cell lysates, <br> Lane 8: mouse ANA-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AB1 antigen affinity purified polyclonal antibody (Catalog # PB9636) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AB1 at approximately 90 kDa. The expected band size for HSP90AB1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9636-2-IHC-anti-hsp90-beta-picoband-antibody.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636). <br> HSP90AB1 was detected in paraffin-embedded section of mouse testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9636-3-IHC-anti-hsp90-beta-picoband-antibody.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636). <br> HSP90AB1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9636-4-IHC-anti-hsp90-beta-picoband-antibody.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IHC analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636). <br> HSP90AB1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9636-5.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IF analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636) <br> HSP90AB1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9636-6.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IF analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636) <br> HSP90AB1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9636-7.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IF analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636) <br> HSP90AB1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9636-8.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IF analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636) <br> HSP90AB1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9636-9.jpgAnti-Hsp90 beta/HSP90AB1 Antibody Picoband&reg; IF analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636).<br> HSP90AB1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSP90AB1 Antibody (PB9636) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grp94-picoband-trade-antibody-pb9637-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-fnagi-17-1568337-g007.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg;Validation of the pyroptosis-AD hub genes at the level of RNA and protein in AD mice. (A) A hierarchical clustering heatmap based on the normalized expression of the five pyroptosis-AD genes in the combined dataset. (B) A clustering heatmap was constructed based on the normalized expression of the five pyroptosis-AD genes in the 6- and 12-month-old APP/PS1 and control mice. The 6- and 12-month-old APP/PS1 or WT mice were abbreviated as A6 and A12 or W6 and W12, respectively. (C–G) qPCR validation of mRNA expression of the pyroptosis-AD hub genes (Chmp2a, Egfr, Pkn2, Hsp90b1, and Mdh1, respectively) between the 12 months APP/PS1 and wild-type (WT) mice. Data are mean ± SEM ( n = 6 for WT, and n = 5 for APP/PS1 mice group, * p < 0.05, ** p < 0.01, unpaired two-tailed t -test). (H–K) The cell lysates from the hippocampus of APP/PS1 and WT mice were prepared and blotted with anti-Egfr, Pkn2, Hsp90b1, and Mdh1, respectively (up). The relative protein expressions of Egfr, Pkn2, Hsp90b1, and Mdh1 were calculated using Gapdh as an internal reference (below). Data are mean ± SEM ( n = 6 per group, * p < 0.05, ** p < 0.01, unpaired two-tailed t -test).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2025.1568337/full'>40438507</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-fnagi-17-1568337-g009.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg;Construction of lncRNA regulatory network of the pyroptosis-AD hub genes. (A) PCA of lncRNAs expression profiles of the APP/PS1 and WT mice at the age of 3, 6, and 12 months. (B) Visualization of the clustered volcano diagram for the DElncRs from six different comparisons, including APP/PS1 mice vs. WT mice at the age of 3, 6, and 12 months and comparison of APP/PS1 mice between different ages. (C) A hierarchical clustering heatmap based on the normalized expression in all samples of DElncRs. The 3-, 6-, and 12-month-old APP/PS1 or WT mice were abbreviated as A3, A6, and A12 or W3, W6, and W12, respectively. (D) The clustered heatmap was produced based on the membership scores of the six clusters obtained by time series analysis. All the DElncRs and five pyroptosis-AD hub genes were clustered into six groups. (E) Line charts showed the relative expression trend in each cluster. The five pyroptosis-AD hub genes were divided into cluster 2 (Champ2 and Mdh1), cluster 3 (Pkn2), and cluster (Egfr and Hsp90b1). The horizontal axis represents a total of nine samples in the age 3-, 6-, and 12-month groups in turn. (F) The heatmaps of correlation analysis of the five pyroptosis-AD hub genes and DElncRs. (G) Regulatory networks constructed by the five pyroptosis-AD hub genes and their top10 (show all if the numbers of lncRNA less than 10) correlated lncRNAs (the ID of lncRNAs could be queried in the NONCODE, NCBI, or Ensemble databases).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/aging-neuroscience/articles/10.3389/fnagi.2025.1568337/full'>40438507</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-grp94-primary-antibodies-wb-testing-1.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; Western blot analysis of GRP94 using anti-GRP94 antibody (PB9637). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human U-87MG whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: huamn CACO-2 whole cell lysates, <br> Lane 5: huamn THP-1 whole cell lysates, <br> Lane 6: huamn CACO-2 whole cell lysates, <br> Lane 7: huamn HepG2 whole cell lysates, <br> Lane 8: huamn HL-60 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP94 antigen affinity purified polyclonal antibody (Catalog # PB9637) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP94 at approximately 100 kDa. The expected band size for GRP94 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-grp94-primary-antibodies-fcm-testing-7_1.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-GRP94 antibody (PB9637).<br>Overlay histogram showing THP-1 cells stained with PB9637 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRP94 Antibody (PB9637,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-grp94-primary-antibodies-wb-testing-2.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; Western blot analysis of GRP94 using anti-GRP94 antibody (PB9637). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat PC-12 whole cell lysates, <br> Lane 2: rat RH35 whole cell lysates, <br> Lane 3: rat C6 whole cell lysates, <br> Lane 4: mouse HEPA1-6 whole cell lysates, <br> Lane 5: mouse NIH/3T3 whole cell lysates, <br> Lane 6: mouse RAW264.7 whole cell lysates, <br> Lane 7: mosue SP2/0 whole cell lysates, <br> Lane 8: mouse ANA-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP94 antigen affinity purified polyclonal antibody (Catalog # PB9637) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP94 at approximately 100 kDa. The expected band size for GRP94 is at 92 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-grp94-primary-antibodies-ihc-testing-3_1.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; IHC analysis of GRP94 using anti-GRP94 antibody (PB9637). <br> GRP94 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRP94 Antibody (PB9637) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-grp94-primary-antibodies-ihc-testing-4_1.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; IHC analysis of GRP94 using anti-GRP94 antibody (PB9637). <br> GRP94 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRP94 Antibody (PB9637) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-grp94-primary-antibodies-ihc-testing-5.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; IHC analysis of GRP94 using anti-GRP94 antibody (PB9637). <br> GRP94 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GRP94 Antibody (PB9637) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9637-grp94-primary-antibodies-if-testing-6.jpgAnti-GRP94/HSP90B1 Antibody Picoband&reg; IF analysis of GRP94 using anti-GRP94 antibody (PB9637). <br> GRP94 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GRP94 Antibody (PB9637) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsp70-picoband-trade-antibody-pb9638-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9638-hsp70-primary-antibodies-wb-testing-1_1.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; Western blot analysis of Hsp70 using anti-Hsp70 antibody (PB9638). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: huamn CACO-2 whole cell lysates, <br> Lane 5: huamn SW620 whole cell lysates, <br> Lane 6: huamn PANC-1 whole cell lysates, <br> Lane 7: huamn Raji whole cell lysates, <br> Lane 8: rat brain tissue lysates, <br> Lane 9: rat kidney tissue lysates, <br> Lane 10: mosue kidney tissue lysates, <br> Lane 11: mouse NIH/3T3 whole cell lysates, <br> Lane 12: mosue RAW264.7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antigen affinity purified polyclonal antibody (Catalog # PB9638) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp70 at approximately 70 kDa. The expected band size for Hsp70 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9638-2-IHC-anti-hsp70-picoband-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IHC analysis of Hsp70 using anti-Hsp70 antibody (PB9638). <br> Hsp70 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp70 Antibody (PB9638) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9638-3-IHC-anti-hsp70-picoband-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IHC analysis of Hsp70 using anti-Hsp70 antibody (PB9638). <br> Hsp70 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp70 Antibody (PB9638) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9638-4-IHC-anti-hsp70-picoband-antibody.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IHC analysis of Hsp70 using anti-Hsp70 antibody (PB9638). <br> Hsp70 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsp70 Antibody (PB9638) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9638-hsp70-primary-antibodies-if-testing-5.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; IF analysis of Hsp70 using anti-Hsp70 antibody (PB9638). <br> Hsp70 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Hsp70 Antibody (PB9638) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9638-hsp70-primary-antibodies-fcm-testing-6_1.jpgAnti-Hsp70/HSPA1A/HSPA1B Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-Hsp70 antibody (PB9638).<br>Overlay histogram showing Hela cells stained with PB9638 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp70 Antibody (PB9638,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hspa2-picoband-trade-antibody-pb9639-boster.html2026-03-24T05:05:12+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9639-hspa2-primary-antibodies-wb-testing-1_1.jpgAnti-HSPA2 Antibody Picoband&reg; Western blot analysis of HSPA2 using anti-HSPA2 antibody (PB9639). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: rat kidney tissue lysates, <br> Lane 5: rat C6 whole cell lysates, <br> Lane 6: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA2 antigen affinity purified polyclonal antibody (Catalog # PB9639) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA2 at approximately 70 kDa. The expected band size for HSPA2 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9639-2-IHC-anti-hspa2-picoband-antibody.jpgAnti-HSPA2 Antibody Picoband&reg; IHC analysis of HSPA2 using anti-HSPA2 antibody (PB9639). <br> HSPA2 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSPA2 Antibody (PB9639) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9639-3-IHC-anti-hspa2-picoband-antibody.jpgAnti-HSPA2 Antibody Picoband&reg; IHC analysis of HSPA2 using anti-HSPA2 antibody (PB9639). <br> HSPA2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSPA2 Antibody (PB9639) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9639-4-IHC-anti-hspa2-picoband-antibody.jpgAnti-HSPA2 Antibody Picoband&reg; IHC analysis of HSPA2 using anti-HSPA2 antibody (PB9639). <br> HSPA2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSPA2 Antibody (PB9639) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9639-5.pngAnti-HSPA2 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-HSPA2 antibody (PB9639). <br> Overlay histogram showing PC-3 cells stained with PB9639 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA2 Antibody (PB9639&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9639-6.jpgAnti-HSPA2 Antibody Picoband&reg; IF analysis of HSPA2 using anti-HSPA2 antibody (PB9639).<br> HSPA2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSPA2 Antibody (PB9639) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9639-7.jpgAnti-HSPA2 Antibody Picoband&reg; IF analysis of HSPA2 using anti-HSPA2 antibody (PB9639).<br> HSPA2 was detected in immunocytochemical section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HSPA2 Antibody (PB9639) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grp78-bip-picoband-trade-antibody-pb9640-boster.html2026-03-26T05:20:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9640-hspa5-primary-antibodies-wb-testing-1.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;Western blot analysis of HSPA5 using anti-HSPA5 antibody (PB9640). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human A431 whole cell lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse testis tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA5 antigen affinity purified polyclonal antibody (PB9640) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HSPA5 at approximately 78 kDa. The expected band size for HSPA5 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9640-hspa5-primary-antibodies-ihc-testing-1.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;IHC analysis of HSPA5 using anti-HSPA5 antibody (PB9640). <br>HSPA5 was detected in a paraffin-embedded section of human cerebral cortex tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (PB9640) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9640-hspa5-primary-antibodies-ihc-testing-2.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;IHC analysis of HSPA5 using anti-HSPA5 antibody (PB9640). <br>HSPA5 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (PB9640) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9640-hspa5-primary-antibodies-ihc-testing-3.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;IHC analysis of HSPA5 using anti-HSPA5 antibody (PB9640). <br>HSPA5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSPA5 Antibody (PB9640) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9640-hspa5-primary-antibodies-if-testing-1.jpgAnti-GRP78 BiP/HSPA5 Antibody Picoband&reg;IF analysis of HSPA5 using anti-HSPA5 antibody (PB9640). <br> HSPA5 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HSPA5 Antibody (PB9640) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsc70-picoband-trade-antibody-pb9641-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9641-hsc70-primary-antibodies-wb-testing-1.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; Western blot analysis of Hsc70 using anti-Hsc70 antibody (PB9641). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human THP-1 whole cell lysates,<br> Lane 6: human K562 whole cell lysates,<br> Lane 7: human PC-3 whole cell lysates,<br> Lane 8: human U87MG whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsc70 antigen affinity purified polyclonal antibody (Catalog # PB9641) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsc70 at approximately 71 kDa. The expected band size for Hsc70 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9641-2-IHC-anti-hsc70-picoband-antibody.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; IHC analysis of Hsc70 using anti-Hsc70 antibody (PB9641). <br> Hsc70 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsc70 Antibody (PB9641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9641-3-IHC-anti-hsc70-picoband-antibody.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; IHC analysis of Hsc70 using anti-Hsc70 antibody (PB9641). <br> Hsc70 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsc70 Antibody (PB9641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9641-4-IHC-anti-hsc70-picoband-antibody.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; IHC analysis of Hsc70 using anti-Hsc70 antibody (PB9641). <br> Hsc70 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Hsc70 Antibody (PB9641) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9641-5.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; IF analysis of Hsc70 using anti-Hsc70 antibody (PB9641). <br> Hsc70 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Hsc70 Antibody (PB9641) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9641-hsc70-primary-antibodies-ip-testing-1.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg;Immunoprecipitating (IP) Hsc70 in A549 whole cell lysate.<br> Western blot analysis of Hsc70 using anti-Hsc70 antibody (PB9641); <br> Lane 1: A549 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Hsc70 antibody in A549 whole cell lysate;<br> Lane 3: anti-Hsc70 antibody (2μg) + A549 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Hsc70 antigen affinity purified polyclonal antibody (PB9641) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Hsc70 at approximately 71 kDa. The expected band size for Hsc70 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9641-6.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-Hsc70 antibody (PB9641).<br>Overlay histogram showing K562 cells stained with PB9641 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsc70 Antibody (PB9641,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9641-hsc70-primary-antibodies-wb-testing-7.jpgAnti-Hsc70/HSPA8 Antibody Picoband&reg; Western blot analysis of Hsc70 using anti-Hsc70 antibody (PB9641). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat spleen tissue lysates, <br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat kidney tissue lysates,<br> Lane 5: mouse liver tissue lysates,<br> Lane 6: mouse spleen tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsc70 antigen affinity purified polyclonal antibody (Catalog # PB9641) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsc70 at approximately 71 kDa. The expected band size for Hsc70 is at 71 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grp75-picoband-trade-antibody-pb9642-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9642-grp75-primary-antibodies-wb-testing-1.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; Western blot analysis of Grp75 using anti-Grp75 antibody (PB9642). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: rat testis tissue lysates, <br> Lane 6: rat PC-12 whole cell lysates, <br> Lane 7: mouse testis tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Grp75 antigen affinity purified polyclonal antibody (Catalog # PB9642) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Grp75 at approximately 74 kDa. The expected band size for Grp75 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9642-grp75-primary-antibodies-ihc-testing-2.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; IHC analysis of Grp75 using anti-Grp75 antibody (PB9642). <br> Grp75 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Grp75 Antibody (PB9642) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9642-grp75-primary-antibodies-ip-testing-1.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg;Immunoprecipitating (IP) Grp75 in A549 whole cell lysate.<br> Western blot analysis of Grp75 using anti-Grp75 antibody (PB9642); <br> Lane 1: A549 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Grp75 antibody in A549 whole cell lysate;<br> Lane 3: anti-Grp75 antibody (2μg) + A549 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Grp75 antigen affinity purified polyclonal antibody (PB9642) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Grp75 at approximately 74 kDa. The expected band size for Grp75 is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9642-grp75-primary-antibodies-ihc-testing-3.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; IHC analysis of Grp75 using anti-Grp75 antibody (PB9642). <br> Grp75 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Grp75 Antibody (PB9642) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9642-grp75-primary-antibodies-if-testing-4.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; IF analysis of Grp75 using anti-Grp75 antibody (PB9642). <br> Grp75 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Grp75 Antibody (PB9642) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9642-grp75-primary-antibodies-fcm-testing-5_1.jpgAnti-Grp75/HSPA9 Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-Grp75 antibody (PB9642). <br> Overlay histogram showing HL-60 cells stained with PB9642 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Grp75 Antibody (PB9642, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ikaros-picoband-trade-antibody-pb9643-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9643-ikzf1-primary-antibody-wb-testing-1.jpgAnti-Ikaros/IKZF1 Antibody Picoband&reg; Western blot analysis of Ikaros using anti-Ikaros antibody (PB9643). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ikaros antigen affinity purified polyclonal antibody (Catalog # PB9643) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ikaros at approximately 50-70 kDa. The expected band size for Ikaros is at 50-70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9643-2-IHC-anti-ikaros-picoband-antibody.jpgAnti-Ikaros/IKZF1 Antibody Picoband&reg; IHC analysis of Ikaros using anti-Ikaros antibody (PB9643). <br> Ikaros was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Ikaros Antibody (PB9643) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9643-3-IHC-anti-ikaros-picoband-antibody.jpgAnti-Ikaros/IKZF1 Antibody Picoband&reg; IHC analysis of Ikaros using anti-Ikaros antibody (PB9643). <br> Ikaros was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Ikaros Antibody (PB9643) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9643-4-IHC-anti-ikaros-picoband-antibody.jpgAnti-Ikaros/IKZF1 Antibody Picoband&reg; IHC analysis of Ikaros using anti-Ikaros antibody (PB9643). <br> Ikaros was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Ikaros Antibody (PB9643) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9643-ikzf1-primary-antibody-if-testing-5.jpgAnti-Ikaros/IKZF1 Antibody Picoband&reg; IF analysis of Ikaros using anti-Ikaros2 antibody (PB9643). <br> Ikaros was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Ikaros Antibody (PB9643) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9643-ikzf1-primary-antibody-fcm-testing-6.pngAnti-Ikaros/IKZF1 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-Ikaros antibody (PB9643). <br>Overlay histogram showing U937 cells stained with PB9643 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ikaros Antibody (PB9643, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ing1-picoband-trade-antibody-pb9644-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9644-1-WB-anti-ing1-picoband-antibody.jpgAnti-ING1 Antibody Picoband&reg; Western blot analysis of ING1 using anti-ING1 antibody (PB9644). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug,<br> Lane 2: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ING1 antigen affinity purified polyclonal antibody (Catalog # PB9644) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ING1 at approximately 47 kDa. The expected band size for ING1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irf2-picoband-trade-antibody-pb9645-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9645-1-WB-anti-irf2-picoband-antibody.jpgAnti-IRF2 Antibody Picoband&reg; Western blot analysis of IRF2 using anti-IRF2 antibody (PB9645). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Intestine Tissue Lysate at 50ug,<br> Lane 2: SW620 Whole Cell Lysate at 40ug,<br> Lane 3: COLO320 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug,<br> Lane 5: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF2 antigen affinity purified polyclonal antibody (Catalog # PB9645) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF2 at approximately 50 kDa. The expected band size for IRF2 is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-irf5-picoband-trade-antibody-pb9646-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9646-1-WB-anti-irf5-picoband-antibody.jpgAnti-IRF5 Antibody Picoband&reg; Western blot analysis of IRF5 using anti-IRF5 antibody (PB9646). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Intestine Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: COLO320 Whole Cell Lysate at 40ug,<br> Lane 4: NIH3T3 Whole Cell Lysate at 40ug,<br> Lane 5: HEPA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF5 antigen affinity purified polyclonal antibody (Catalog # PB9646) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF5 at approximately 56 kDa. The expected band size for IRF5 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9646-2-IHC-anti-irf5-picoband-antibody.jpgAnti-IRF5 Antibody Picoband&reg; IHC analysis of IRF5 using anti-IRF5 antibody (PB9646). <br> IRF5 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-IRF5 Antibody (PB9646) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9646-3-IHC-anti-irf5-picoband-antibody.jpgAnti-IRF5 Antibody Picoband&reg; IHC analysis of IRF5 using anti-IRF5 antibody (PB9646). <br> IRF5 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-IRF5 Antibody (PB9646) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9646-4-IHC-anti-irf5-picoband-antibody.jpgAnti-IRF5 Antibody Picoband&reg; IHC analysis of IRF5 using anti-IRF5 antibody (PB9646). <br> IRF5 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-IRF5 Antibody (PB9646) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-itga2b-picoband-trade-antibody-pb9647-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9647-itga2b-primary-antibodies-wb-testing-1.jpgAnti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband&reg; Western blot analysis of ITGA2B using anti-ITGA2B antibody (PB9647). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HEL whole cell lysates,<br> Lane 2: human HEL whole cell lysates,<br> Lane 3: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGA2B antigen affinity purified polyclonal antibody (Catalog # PB9647) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGA2B at approximately 120-135 kDa. The expected band size for ITGA2B is at 113 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9647-41598_2017_12073_fig3_html.jpgAnti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband&reg;The effects of rhTyrRS(Y341A) on megakaryocytes were studied by immunohistochemistry and ( H & E ) staining in the bone marrow and lung. ( H & E ) staining showed there was no increase in the number of bone marrow megakaryocyte cells in the ( B ) rhTyrRS (Y341A) (100 μg/kg) group (22.0 ± 1.2) or ( A ) PBS group (21 ± 1.25). However, the number of bone marrow megakaryocyte cells increased significantly in ( C ) the TPO group (33.5 ± 1.0) (***p < 0.001). Immunohistochemistry of bone marrow megakaryocytes (CD41 + ) also showed there was no increase in the number of bone marrow megakaryocyte cells in the ( E ) rhTyrRS (Y341A) (100 μg/kg) (23.3 ± 0.8) or ( D ) PBS group (22.3 ± 0 0.67), while the number of bone marrow megakaryocyte cells increased significantly in the ( F ) TPO group (35.2 ± 0.9) (***p < 0.001), mean ± SEM. ( G ) Statistics on the number of bone marrow megakaryocytes in H&E staining and immunohistochemistry (***P < 0.001). Lung ( H & E ) staining showed there was no increase in the number of lung megakaryocyte cells in the ( H ) PBS group (3.67 ± 0.49), while the number of lung megakaryocyte cells increased significantly in the ( I ) rhTyrRS (Y341A) (100 μg/kg) (7.33 ± 0.56) and ( J ) TPO group (6.33 ± 0.84) (***p < 0.001,**p < 0.01). Immunohistochemistry of lung megakaryocytes (CD41 + ) also showed there was no increase in the number of lung megakaryocyte cells in the ( K ) PBS group (3.17 ± 0.48), while the number of lung megakaryocyte cells increased significantly in the ( L ) rhTyrRS (Y341A) (100 μg/kg) (6.5 ± 0.43) and ( M ) TPO group (6.3 ± 0.42) (***p < 0.001), mean ± SEM. ( N ) Statistics on the megakaryocyte numbers in lungs using H&E staining and immunohistochemistry (***P < 0.001, **p < 0.01). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-017-12073-4'>28939876</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9647-2-IHC-anti-itga2b-cd41-picoband-antibody.jpgAnti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband&reg; IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647). <br> ITGA2B was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9647-3-IHC-anti-itga2b-cd41-picoband-antibody.jpgAnti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband&reg; IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647). <br> ITGA2B was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9647-4-IHC-anti-itga2b-cd41-picoband-antibody.jpgAnti-CD41/Integrin alpha 2b/ITGA2B Antibody Picoband&reg; IHC analysis of ITGA2B using anti-ITGA2B antibody (PB9647). <br> ITGA2B was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITGA2B Antibody (PB9647) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-alpha-4-picoband-trade-antibody-pb9648-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9648-1-WB-anti-integrin-alpha-4-picoband-antibody.jpgAnti-Integrin alpha 4/ITGA4 Antibody Picoband&reg; Western blot analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody (PB9648). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Rat Kidney Tissue Lysate at 50ug,<br> Lane 3: JURKAT Whole Cell Lysate at 40ug,<br> Lane 4: 22RV1 Whole Cell Lysate at 40ug,<br> Lane 5: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 6: SMMC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 4 antigen affinity purified polyclonal antibody (Catalog # PB9648) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 4 at approximately 220 kDa. The expected band size for Integrin alpha 4 is at 115 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9648-2-IHC-anti-integrin-alpha-4-picoband-antibody.jpgAnti-Integrin alpha 4/ITGA4 Antibody Picoband&reg; IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody (PB9648). <br> Integrin alpha 4 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Integrin alpha 4 Antibody (PB9648) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9648-3.jpgAnti-Integrin alpha 4/ITGA4 Antibody Picoband&reg; IHC analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody (PB9648).<br> Integrin alpha 4 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Integrin alpha 4 Antibody (PB9648) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9648-4.pngAnti-Integrin alpha 4/ITGA4 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-Integrin alpha 4 antibody (PB9648). <br> Overlay histogram showing THP-1 cells stained with PB9648 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin alpha 4 Antibody (PB9648&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kv1-2-picoband-trade-antibody-pb9649-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9649-1-WB-anti-kv1-2-picoband-antibody.jpgAnti-Kv1.2/KCNA2 Antibody Picoband&reg; Western blot analysis of Kv1.2 using anti-Kv1.2 antibody (PB9649). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Kidney Tissue Lysate at 50ug,<br> Lane 2: Rat Brain Tissue Lysate at 50ug,<br> Lane 3: Mouse Brain Tissue Lysate at 50ug,<br> Lane 4: Mouse Kidney Tissue Lysate at 50ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.2 antigen affinity purified polyclonal antibody (Catalog # PB9649) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv1.2 at approximately 57 kDa. The expected band size for Kv1.2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9649-kv1.2-primary-antibodies-ihc-testing-1.jpgAnti-Kv1.2/KCNA2 Antibody Picoband&reg;IHC analysis of Kv1.2 using anti-Kv1.2 antibody (PB9649). <br>Kv1.2 was detected in a paraffin-embedded section of human cerebral cortex and hippocampus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Kv1.2 Antibody (PB9649) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9649-2.jpgAnti-Kv1.2/KCNA2 Antibody Picoband&reg; IF analysis of Kv1.2 using anti-Kv1.2 antibody (PB9649). <br> Kv1.2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Kv1.2 Antibody (PB9649) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9649-3.jpgAnti-Kv1.2/KCNA2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-Kv1.2 antibody (PB9649).<br>Overlay histogram showing A431 cells stained with PB9649 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Kv1.2 Antibody (PB9649,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9649-4.jpgAnti-Kv1.2/KCNA2 Antibody Picoband&reg; Flow Cytometry analysis of C6 cells using anti-Kv1.2 antibody (PB9649).<br>Overlay histogram showing C6 cells stained with PB9649 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Kv1.2 Antibody (PB9649,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kcna3-picoband-trade-antibody-pb9650-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9650-1-WB-anti-kcna3-kv1-3-picoband-antibody.jpgAnti-KCNA3 Antibody Picoband&reg; Western blot analysis of KCNA3 using anti-KCNA3 antibody (PB9650). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: K562 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug,<br> Lane 5: 22RV1 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA3 antigen affinity purified polyclonal antibody (Catalog # PB9650) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA3 at approximately 55 kDa. The expected band size for KCNA3 is at 64 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kcna5-picoband-trade-antibody-pb9651-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9651-1-WB-anti-kcna5-kv1-5-picoband-antibody.jpgAnti-KCNA5 Antibody Picoband&reg; Western blot analysis of KCNA5 using anti-KCNA5 antibody (PB9651). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: 293T Whole Cell Lysate at 40ug,<br> Lane 2: A549 Whole Cell Lysate at 40ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA5 antigen affinity purified polyclonal antibody (Catalog # PB9651) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA5 at approximately 67 kDa. The expected band size for KCNA5 is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kchip2-picoband-trade-antibody-pb9652-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9652-1-WB-anti-kchip2-picoband-antibody.jpgAnti-KChIP2/KCNIP2 Antibody Picoband&reg; Western blot analysis of KCNA5 using anti-KCNA5 antibody (PB9652). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 4: 22RV1 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA5 antigen affinity purified polyclonal antibody (Catalog # PB9652) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KCNA5 at approximately 31 kDa. The expected band size for KCNA5 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9652-2-IHC-anti-kchip2-picoband-antibody.jpgAnti-KChIP2/KCNIP2 Antibody Picoband&reg; IHC analysis of KCNA5 using anti-KCNA5 antibody (PB9652). <br> KCNA5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KCNA5 Antibody (PB9652) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9652-3-IHC-anti-kchip2-picoband-antibody.jpgAnti-KChIP2/KCNIP2 Antibody Picoband&reg; IHC analysis of KCNA5 using anti-KCNA5 antibody (PB9652). <br> KCNA5 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KCNA5 Antibody (PB9652) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9652-4-IHC-anti-kchip2-picoband-antibody.jpgAnti-KChIP2/KCNIP2 Antibody Picoband&reg; IHC analysis of KCNA5 using anti-KCNA5 antibody (PB9652). <br> KCNA5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KCNA5 Antibody (PB9652) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9652-5.jpgAnti-KChIP2/KCNIP2 Antibody Picoband&reg; IF analysis of KChIP2 using anti-KChIP2 antibody (PB9652). <br> KChIP2 was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-KChIP2 Antibody (PB9652) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9652-6.jpgAnti-KChIP2/KCNIP2 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-KChIP2 antibody (PB9652).<br>Overlay histogram showing K562 cells stained with PB9652 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KChIP2 Antibody (PB9652,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-keratocan-picoband-trade-antibody-pb9653-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9653-1-WB-anti-keratocan-picoband-antibody.jpgAnti-Keratocan Antibody Picoband&reg; Western blot analysis of Keratocan using anti-Keratocan antibody (PB9653). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Mouse Testis Whole Cell Lysate at 40ug,<br> Lane 2: Mouse Skeletal Muscle Whole Cell Lysate at 40ug,<br> Lane 3: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 4: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keratocan antigen affinity purified polyclonal antibody (Catalog # PB9653) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keratocan at approximately 50 kDa. The expected band size for Keratocan is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kif3a-picoband-trade-antibody-pb9654-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9654-kif3a-primary-antibodies-wb-testing-1.jpgAnti-KIF3A Antibody Picoband&reg; Western blot analysis of KIF3A using anti-KIF3A antibody (PB9654). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human SH-SY5Y whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse Neuro-2a whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIF3A antigen affinity purified polyclonal antibody (PB9654) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for KIF3A at approximately 80 kDa. The expected band size for KIF3A is at 80 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kininogen-1-picoband-trade-antibody-pb9655-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9655-1-WB-anti-kininogen-1-kng1-antibody.jpgAnti-Kininogen 1/KNG1 Antibody Picoband&reg; Western blot analysis of Kininogen 1 using anti-Kininogen 1 antibody (PB9655). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Mouse Lung Tissue Lysate at 50ug,<br> Lane 2: Mouse Testis Tissue Lysate at 50ug,<br> Lane 3: Mouse Liver Tissue Lysate at 50ug,<br> Lane 4: HEPA Whole Cell Lysate at 40ug,<br> Lane 5: NEURO Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kininogen 1 antigen affinity purified polyclonal antibody (Catalog # PB9655) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kininogen 1 at approximately 73 kDa. The expected band size for Kininogen 1 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9655-2-IHC-anti-kininogen-1-kng1-antibody.jpgAnti-Kininogen 1/KNG1 Antibody Picoband&reg; IHC analysis of Kininogen 1 using anti-Kininogen 1 antibody (PB9655). <br> Kininogen 1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Kininogen 1 Antibody (PB9655) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lcat-picoband-trade-antibody-pb9657-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9657-1_1-WB-anti-lcat-picoband-antibody.jpgAnti-LCAT Antibody Picoband&reg; Western blot analysis of LCAT using anti-LCAT antibody (PB9657). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Testis Tissue Lysate at 50ug,<br> Lane 3: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LCAT antigen affinity purified polyclonal antibody (Catalog # PB9657) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LCAT at approximately 50 kDa. The expected band size for LCAT is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-leptin-picoband-trade-antibody-pb9658-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9658-1-WB-anti-leptin-picoband-antibody.jpgAnti-Leptin Antibody Picoband&reg; Western blot analysis of Leptin using anti-Leptin antibody (PB9658). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: NIH3T3 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Leptin antigen affinity purified polyclonal antibody (Catalog # PB9658) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Leptin at approximately 16 kDa. The expected band size for Leptin is at 16 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-galectin-8-picoband-trade-antibody-pb9659-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9659-1_1.jpgAnti-Galectin 8/LGALS8 Antibody Picoband&reg; Western blot analysis of Galectin 8 using anti-Galectin 8 antibody (PB9659). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human COLO-320 whole cell lysates<br> Lane 2: rat testicular tissue lysates<br> Lane 3: mouse Neuro-2A whole cell lysates <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 8 antigen affinity purified polyclonal antibody (Catalog # PB9659) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 8 at approximately 36KD. The expected band size for Galectin 8 is at 36KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lifr-picoband-trade-antibody-pb9661-boster.html2026-03-24T05:05:13+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9661-1-WB-anti-lifr-lif-r-alpha-picoband-antibody.jpgAnti-LIFR Antibody Picoband&reg; Western blot analysis of LIFR using anti-LIFR antibody (PB9661). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: SW620 Whole Cell Lysate at 40ug,<br> Lane 2: COLO320 Whole Cell Lysate at 40ug,<br> Lane 3: HEPG2 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIFR antigen affinity purified polyclonal antibody (Catalog # PB9661) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIFR at approximately 190 kDa. The expected band size for LIFR is at 190 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lysozyme-picoband-trade-antibody-pb9663-boster.html2026-04-03T05:00:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9663-lysozyme-primary-antibodies-wb-testing-1.jpgAnti-Lysozyme/LYZ Antibody Picoband&reg; Western blot analysis of Lysozyme using anti-Lysozyme antibody (PB9663). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human HL-60 whole cell lysates,<br> Lane 3: rat lung tissue lysates.<br> Lane 4: rat PC-12 whole cell lysates.<br> Lane 5: mouse lung tissue lysates.<br> Lane 6: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme antigen affinity purified polyclonal antibody (Catalog # PB9663) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lysozyme at approximately 15-17 kDa. The expected band size for Lysozyme is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9663-2-IHC-anti-lysozyme-picoband-antibody.jpgAnti-Lysozyme/LYZ Antibody Picoband&reg; IHC analysis of Lysozyme using anti-Lysozyme antibody (PB9663). <br> Lysozyme was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9663-lysozyme-primary-antibodies-if-testing-3.pngAnti-Lysozyme/LYZ Antibody Picoband&reg; IF analysis of Lysozyme using anti-Lysozyme antibody (PB9663). <br> Lysozyme was detected in paraffin-embedded section of human ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9663-lysozyme-primary-antibodies-if-testing-4.pngAnti-Lysozyme/LYZ Antibody Picoband&reg; IF analysis of Lysozyme using anti-Lysozyme antibody (PB9663). <br> Lysozyme was detected in paraffin-embedded section of human colon organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9663-lysozyme-primary-antibodies-if-testing-5.pngAnti-Lysozyme/LYZ Antibody Picoband&reg; IF analysis of Lysozyme using anti-Lysozyme antibody (PB9663). <br> Lysozyme was detected in paraffin-embedded section of mouse ileum tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9663-lysozyme-primary-antibodies-if-testing-6.pngAnti-Lysozyme/LYZ Antibody Picoband&reg; IF analysis of Lysozyme using anti-Lysozyme antibody (PB9663). <br> Lysozyme was detected in paraffin-embedded section of mouse ileum organoid tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9663-lyz-primary-antibodies-if-testing-7.jpgAnti-Lysozyme/LYZ Antibody Picoband&reg; IF analysis of Lysozyme using anti-Lysozyme antibody (PB9663). <br> Lysozyme was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4 μg/mL rabbit anti-Lysozyme Antibody (PB9663) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-maoa-picoband-trade-antibody-pb9664-boster.html2026-03-25T05:22:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9664-maoa-primary-antibodies-if-testing-1.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; Western blot analysis of MAOA using anti-MAOA antibody (PB9664). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOA antigen affinity purified polyclonal antibody (Catalog # PB9664) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAOA at approximately 60 kDa. The expected band size for MAOA is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9664-maoa-primary-antibodies-wb-testing-1.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg;Western blot analysis of MAOA using anti-MAOA antibody (PB9664).<br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions.<br> Lane 1: rat spinal cord tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse spinal cord tissue lysates,<br> Lane 4: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOA antigen affinity purified polyclonal antibody (PB9664) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAOA at approximately 60 kDa. The expected band size for MAOA is at 60 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9664-2-IHC-anti-maoa-monoamine-oxidase-a-picoband-antibody.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; IHC analysis of MAOA using anti-MAOA antibody (PB9664). <br> MAOA was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MAOA Antibody (PB9664) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9664-3-IHC-anti-maoa-monoamine-oxidase-a-picoband-antibody.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; IHC analysis of MAOA using anti-MAOA antibody (PB9664). <br> MAOA was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MAOA Antibody (PB9664) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9664-4-IHC-anti-maoa-monoamine-oxidase-a-picoband-antibody.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; IHC analysis of MAOA using anti-MAOA antibody (PB9664). <br> MAOA was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MAOA Antibody (PB9664) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9664-5.pngAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-MAOA antibody (PB9664). <br> Overlay histogram showing U87 cells stained with PB9664 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAOA Antibody (PB9664&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9664-6.pngAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-MAOA antibody (PB9664). <br> Overlay histogram showing U20S cells stained with PB9664 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAOA Antibody (PB9664&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) washttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9664-maoa-primary-antibodies-if-testing-7.jpg.jpgAnti-Monoamine Oxidase A/MAOA Antibody Picoband&reg; IF analysis of MAOA using anti-MAOA antibody (PB9664). <br> MAOA was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MAOA Antibody (PB9664) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-maob-picoband-trade-antibody-pb9665-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9665-maob-primary-antibodies-wb-testing-1_1.jpgAnti-Monoamine Oxidase B/MAOB Antibody Picoband&reg;Western blot analysis of MAOB using anti-MAOB antibody (PB9665). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SiHa whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human placenta tissue lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat brain tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (PB9665) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9665-maob-primary-antibodies-ihc-testing-2.jpgAnti-Monoamine Oxidase B/MAOB Antibody Picoband&reg;IHC analysis of MAOB using anti-MAOB antibody (PB9665). <br>MAOB was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAOB Antibody (PB9665) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9665-maob-primary-antibodies-ihc-testing-1.jpgAnti-Monoamine Oxidase B/MAOB Antibody Picoband&reg;IHC analysis of MAOB using anti-MAOB antibody (PB9665). <br> MAOB was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MAOB Antibody (PB9665) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9665-maob-primary-antibodies-ip-testing-1.jpgAnti-Monoamine Oxidase B/MAOB Antibody Picoband&reg;Immunoprecipitating (IP) MAOB in Hela whole cell lysate.<br> Western blot analysis of MAOB using anti-MAOB antibody (PB9665); <br> Lane 1: Hela whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-MAOB antibody in Hela whole cell lysate;<br> Lane 3: anti-MAOB antibody (2μg) + Hela whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (PB9665) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mapk6-picoband-trade-antibody-pb9666-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9666-1-WB-anti-mapk6-erk3-picoband-antibody.jpgAnti-MAPK6 Antibody Picoband&reg; Western blot analysis of MAPK6 using anti-MAPK6 antibody (PB9666). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug,<br> Lane 4: NIH3T3 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK6 antigen affinity purified polyclonal antibody (Catalog # PB9666) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPK6 at approximately 100 kDa. The expected band size for MAPK6 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9666-2-IHC-anti-mapk6-erk3-picoband-antibody.jpgAnti-MAPK6 Antibody Picoband&reg; IHC analysis of MAPK6 using anti-MAPK6 antibody (PB9666). <br> MAPK6 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MAPK6 Antibody (PB9666) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mefv-picoband-trade-antibody-pb9667-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9667-1_1-WB-anti-mefv-picoband-antibody.jpgAnti-MEFV Antibody Picoband&reg; Western blot analysis of MEFV using anti-MEFV antibody (PB9667). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Spleen Tissue Lysate at 50ug,<br> Lane 2: Rat Lung Tissue Lysate at 50ug,<br> Lane 3: HEPA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEFV antigen affinity purified polyclonal antibody (Catalog # PB9667) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEFV at approximately 86 kDa. The expected band size for MEFV is at 86 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp-9-picoband-trade-antibody-pb9668-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-12958_2022_952_fig3_html.pngAnti-MMP9 Antibody Picoband&reg;miR-135a-5p mimics suppress migratory and invasive activity in HTR-8/SVneo cells. ( a - b ) Overexpressing miR-135a-5p inhibited HTR-8/SVneo cell migration in a wound healing assay. ( c - e ) Overexpressing miR-135a-5p suppressed HTR-8/SVneo cell migration and invasion in Transwell assessments. ( f - g ) Western blotting revealed E-cad upregulation and the downregulation of MMP2, MMP9, and Vimentin, with GAPDH as a reference control. Data are means ± SD from at least three experiments. E-cad: E-cadherin. * P < 0.05, ** P < 0.01, *** P < 0.001 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12958-022-00952-z'>35610725</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-10020_2022_433_fig2_html.pngAnti-MMP9 Antibody Picoband&reg;Pam3 CM up-regulates fibrogenic and osteogenic mediators in AVICs independent of TLR2. A–C AVICs were exposed to Pam3 (0.03 μg/ml), control CM or Pam3 CM for 48 h. Levels of collagen I, collagen IV, MMP-2, MMP-9, ALP and RUNX2 were determined by immunoblotting. Representative immunoblots and densitometric data show that Pam3 CM selectively up-regulated collagen I, MMP-2 and ALP in AVICs among the fibrogenic and osteogenic factors examined while control CM or a low concentration of Pam3 had no effect. D AVICs were pretreated with TLR2 inhibitor CU CPT 22 (10 µM) or DMSO for 1 h and then exposed to Pam3 CM treatment for 48 h. Inhibition of TLR2 in AVICs did not alter the effect of Pam3 CM on the upregulation of collagen I, MMP-2 and ALP. Data are presented as mean ± SEM. n = 5 cell isolates from distinct donor valves in each group. *P < 0.05 vs. untreated control <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s10020-022-00433-4'>35062861</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-41467_2025_56878_fig1_html.pngAnti-MMP9 Antibody Picoband&reg;Conditioned medium derived from HCC cells grown on the high-stiffness substrate accelerates the formation of the lung pre-metastatic niche. a Schematic illustration of tumor-free mouse models with lung pre-metastatic niches induced by Hepa1-6-L/H-CM, created in BioRender. Zhao, Y. (2025) . b, c Flow cytometry analysis ( b ) and quantification ( c ) of CD11b + CD45 + bone marrow-derived cells (BMDCs) in lung tissues ( n = 2 mice per group on days 6, 14, 18, and 22; n = 4 mice per group on day 26). d qRT-PCR analysis of pre-metastatic niche-related genes in lung tissues on day 26 ( n = 4 mice per group). Data were normalized to β-actin. e IHC staining of fibronectin in lung tissues on day 26 ( n = 4 mice per group). f , g Percentage of CD11b + Gr-1 + myeloid-derived suppressor cells (MDSCs) ( f ) and CD8 + T cells ( g ) in lung tissues on day 26 ( n = 4 mice per group). h IHC staining of CD31 in lung tissues on day 26 ( n = 4 mice per group). Scale bars: black, 200 μm; red, 50 μm ( e , h ). i IF images for CD31 and VE-cadherin in lung tissues on day 26 ( n = 4 mice per group). Scale bars: 20 μm. j Western blot analysis of fibronectin and MMP9 in lung fibroblasts treated with MHCC97H/Hep3B-L/H-CM grown on lung stiffness substrates. k Adherent HCC cells on lung fibroblast monolayer treated with MHCC97H/Hep3B-L/H-CM grown on lung stiffness substrates ( n = 6 biological replicates). Scale bars: 200 μm. l Western blot analysis of ZO-1, VE-cadherin and VEGFR2 in HUVECs treated with MHCC97H/Hep3B-L/H-CM. The samples derive from the same experiment but different gels for ZO-1 and VE-cadherin, and another for VEGFR2 and β-actin were processed in parallel. m Permeability of HUVEC monolayer treated with MHCC97H-L/H-CM to FITC-dextran ( n = 3 biological replicates). No monolayer cells, no HUVECs on the upper chamber. Representative images are presented from indicated biologically independent experiments ( b , e – i , k ). Representative blot (20 μg protein per group) was shown from 3 biologically independent experiments ( j , l ), and β-actin was used to normalize protein quantification. Data are presented as mean ± SD, and P values were calculated using two-tailed unpaired Student’s t-test ( c – m ). L low-stiffness substrates, H high-stiffness substrates, CM conditioned medium, OD498 optical density (OD) measured at 498 nm. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-56878-8'>39966385</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-41467_2025_56878_fig3_html.pngAnti-MMP9 Antibody Picoband&reg;Tumor-derived exosomes as the major contributor modulate glucose enrichment during H-CM-induced lung pre-metastatic niche. a Transmission electron microscope (TEM) images of exosomes purified from MHCC97H/Hep3B-L/H-CM ( n = 3 biological replicates). Scale bars: 50 μm. b Molecule markers of exosomes detected by western blot ( n = 3 biological replicates). The samples derive from the same experiment but different gels for TSG101, CD63, and Hsp70, another for ALIX, and another for Albumin and Cytochrome C were processed in parallel. c Internalization of DIO-labeled exosomes (green) by lung fibroblasts and vascular endothelial cells ( n = 3 biological replicates). Scale bars: 5 μm. d Schematic illustration of a co-culture system in vitro simulating pre-metastatic niche environment (upper panel) and western blot analysis of fibronectin and MMP9 in lung fibroblasts treated with MHCC97H-L/H-Exo in this system (lower panel). Upper panel was created in BioRender. Zhao, Y. (2025) . e Flow cytometry analysis of CD11b + Gr-1 + MDSCs in differentiated bone marrow cells (BMCs) co-cultured with L/H-Exo-treated lung fibroblasts ( n = 3 biological replicates). f Schematic illustration of tumor-free mouse models with lung pre-metastatic niches induced by Hepa1-6-L/H-Exo, created in BioRender. Zhao, Y. (2025) . g Flow cytometry analysis of CD11b + CD45 + BMDCs in the pre-metastatic lung tissues on day 26 ( n = 5 mice per group). h Levels of glucose in the cell-free interstitial fraction fluid from the pre-metastatic lung tissues on day 26 ( n = 5 mice per group). i Multiplex immunofluorescence of TSG101, CD31, and VE-cadherin in the pre-metastatic lung tissues on day 26 ( n = 5 mice per group). Scale bars: 20 μm. j Bioluminescence imaging (BLI) of lung metastasis lesions in lung tissues on day 54 and day 61 ( n = 5 mice per group). k HE staining and quantification of lung metastasis lesions in lung tissues on day 61 ( n = 5 mice per group). Scale bars: red, 2 mm; blue, 500 μm; black, 100 μm. Representative images are presented from indicated biologically independent experiments ( a – c , e , g , i – k ). Representative blot (20 μg protein per group) was shown from 3 biologically independent experiments ( d ), and β-actin was used to normalize protein quantification. Data are presented as mean ± SD, and P values were calculated using one-way ANOVA ( d ) or two-tailed unpaired Student’s t-test ( e , g – i , k ). L low-stiffness substrates, H high-stiffness substrates, Exo exosome. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-56878-8'>39966385</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-fig-1-1x.jpgAnti-MMP9 Antibody Picoband&reg;Regulation of FLT on xenograft EMS. (A) Ectopic volume was detected by vernier caliper in xenograft EMS model. GTN was performed as positive control. (B) Weight of nude mice was measured every 7 days during 28 days’ treatment. * P < 0.05 to pretreatment. Columns, mean ( n = 6). (C–E) Ectopic endometrium of EMS and FLT groups were collected for RNA isolation. Eutopic endometrium of C3H mice without transplantation were supplied as the control group. mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by qPCR in different groups. (F–I) Protein levels of MMP-2, MMP-9, and TIMP-1 were measured by western blotting, and the ratio of MMP-2, MMP-9, and TIMP-1 with β-actin were shown. # P < 0.05 to control, ## P < 0.01 to control, * P < 0.05 to EMS, ** P < 0.01 to EMS. Columns, mean ( n = 3). Bars, SD. EMS, endometriosis; FLT, ferulic acid, ligustrazine and tetrahydropalmatine; GTN, gestrinone. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/11664/'>34249506</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-fig-5-1x.jpgAnti-MMP9 Antibody Picoband&reg;Gene expression of MMP/TIMP signaling adjusted by FLT for 24h. The mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by qPCR in hEM15A (A–C) and HEC1-B (D–F) cells. * P < 0.05 to control group, ** P < 0.01 to control group. Columns, mean ( n = 3). Bars, SD. FLT, ferulic acid, ligustrazine and tetrahydropalmatine. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/11664/'>34249506</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-fig-6-1x.jpgAnti-MMP9 Antibody Picoband&reg;Modification of MMP/TIMP signaling protein treating with FLT for 24h. Protein levels of MMP-2, MMP-9, and TIMP-1 were detected by western blot in hEM15A (A–D) and HEC1-B (E–H) cells. The ratio of MMP-2, MMP-9, and TIMP-1 with β-actin were shown. * P < 0.05 to control group, ** P < 0.01 to control group. Columns, mean ( n = 3). Bars, SD. FLT, ferulic acid, ligustrazine and tetrahydropalmatine. Download full-size image DOI:<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://peerj.com/articles/11664/'>34249506</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-oncotarget-08-63461-g002.jpgAnti-MMP9 Antibody Picoband&reg;Panc02-H7-derived exosomes promote metastatis-related characteristics in vitro . Panc02 cells tookup PKH67-labeled Panc02-H7EXOs. Numerous green fluorescently-labeled exosomes were observed inside cells after 5 h (400× magnification). (A) The MTT cell adhesion assay indicated that Panc02-H7 EXOs decrease Panc02 cell adhesion. (B) Wound-healing assays indicated that Panc02-H7 EXOs enhanced Panc02 cell migration (200×magnification). (C) Transwell chamber invasion assays showed that Panc02-H7 EXOs increased Panc02 cell invasion (200×magnification). (D) Western blotting indicated that Panc02-H7 EXOs increased Panc02 cell migration and invasion via CXCR4 and MMP-9 signaling. (E) n=3/group.*P<0.05,**P<0.01, ***P<0.001 compared to control; #P<0.05, ##P<0.01, ###P<0.001 compared to EXO-D.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5609937/&orig_host=www.ncbi.nlm.nih.gov'>28969005</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-ijcep0007-6040-f1.jpgAnti-MMP9 Antibody Picoband&reg;LOX and MMP2/MMP9 are positively expressed in resected NSCLC tumors. Total RNA extracted from 30 pairs of matched NSCLC samples was used for qRT-PCR analysis of mRNA expression. A. Upregulation of LOX mRNA expression in NSCLC tissues. The results were normalized to β-actin expression and expressed as fold change in tumor compared with matched N ( P < 0.001). B. Positive correlation between LOX mRNA and MMP2 mRNA in NSCLC tissues ( P = 0.002). C. Positive correlation between LOX mRNA and MMP9 mRNA in NSCLC tissues ( P = 0.003). N: non-tumor tissue; T: tumor tissue.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4203220/&orig_host=www.ncbi.nlm.nih.gov'>25337249</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-ijcep0007-6040-f2.jpgAnti-MMP9 Antibody Picoband&reg;Cytoplasm localization of LOX, MMP2 and MMP9 in NSCLC. Immunohistochemistry was performed to examine the expression of LOX, MMP2 and MMP9 protein in tumor tissues (×400). A. Positive expression of LOX protein in NSCLC. B. Positive expression of MMP2 in NSCLC. C. Positive expression of MMP9 in NSCLC. D. Negative expression of LOX protein in NSCLC. E. Negative expression of MMP2 protein in NSCLC. F. Negative expression of MMP9 protein in NSCLC.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4203220/&orig_host=www.ncbi.nlm.nih.gov'>25337249</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-wjg-14-2308-g004.jpgAnti-MMP9 Antibody Picoband&reg;A: Effect of AMD3100 on expression of MMP-2, MMP-9 and VEGF in SW480 cells. Protein samples extracted from SW480 cells treated for 26 h with AMD3100 were subjected to Western blotting for MMP-2, MMP-9, VEGF and GAPDH proteins. AMD3100 significantly decreased MMP-9 and VEGF protein expression in SW480 cells in a dose-dependent manner. The level of GAPDH expression was determined as a control for equivalent protein loading; B: RNA samples extracted from SW480 cells treated for 26 h with AMD3100 were subjected to RT-PCR for MMP-2 , MMP-9 , VEGF and β -actin . AMD3100 also significantly decreased expression of MMP-9 and VEGF mRNAs in SW480 cells, and the inhibitory effect was dose-dependent. RT-PCR for β -actin was performed in parallel to show an equal amount of total RNA in the sample.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC2705083/'>18416455</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-mmp9-primary-antibodies-wb-testing-1.jpgAnti-MMP9 Antibody Picoband&reg; Western blot analysis of MMP9 using anti-MMP9 antibody (PB9668). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP9 antigen affinity purified polyclonal antibody (Catalog # PB9668) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP9 at approximately 79 kDa. The expected band size for MMP9 is at 79 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-mmp9-primary-antibodies-ihc-testing-2.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP9 using anti-MMP9 antibody (PB9668). <br> MMP9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (PB9668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-mmp9-primary-antibodies-ihc-testing-3.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP9 using anti-MMP9 antibody (PB9668). <br> MMP9 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (PB9668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-mmp9-primary-antibodies-ihc-testing-4.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP9 using anti-MMP9 antibody (PB9668). <br> MMP9 was detected in a paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (PB9668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-mmp9-primary-antibodies-ihc-testing-5.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP9 using anti-MMP9 antibody (PB9668). <br> MMP9 was detected in a paraffin-embedded section of human lymph nodes of gastric adenocarcinoma rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (PB9668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9668-mmp9-primary-antibodies-ihc-testing-6.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP9 using anti-MMP9 antibody (PB9668). <br> MMP9 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MMP9 Antibody (PB9668) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp-9-picoband-trade-antibody-pb9669-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9669-1-WB-anti-mmp-9-antibody.jpgAnti-MMP9 Antibody Picoband&reg; Western blot analysis of MMP-9 using anti-MMP-9 antibody (PB9669). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. <br> Lane 1: NRK Whole Cell Lysate,<br> Lane 2: ANA-1 Whole Cell Lysate,<br> Lane 3: HEPA Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP-9 antigen affinity purified polyclonal antibody (Catalog # PB9669) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP-9 at approximately 78 kDa. The expected band size for MMP-9 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9669-2-IHC-anti-mmp-9-antibody.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP-9 using anti-MMP-9 antibody (PB9669). <br> MMP-9 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MMP-9 Antibody (PB9669) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9669-13020_2022_627_fig10_html.pngAnti-MMP9 Antibody Picoband&reg;Effects of JTG on expression of associated proteins and NF-κB pathway of osteoclast induced from BMMs with RANKL and LPS. BMMs were incubated with RANKL and JTG for 48 h, the proteins were extracted to analyze associated proteins of osteoclast by Western blot. A : a Western blot imagines for expression of NFATc1, c-Fos, Cathepsin K and MMP9. A : b – e The quantification analysis of NFATc1, c-Fos, Cathepsin K and MMP9 based on the results of A : a by ECL detection system, respectively. B : a The images of Western blot for TRAF6, P-P65, P65 and IκBα. B : b – d The quantification analysis of TRAF6, P-P65/P65 and IκBα based on the results of B : a by using an ECL detection system, respectively. Each point represents the mean ± SD (n = 3). The experiments were repeated for three times. * P < 0.05, ** P < 0.01 compared with control group <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-022-00627-2'>36195960</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9669-fphar-09-00811-g006.jpgAnti-MMP9 Antibody Picoband&reg;Effect of JFS on invasion and metastasis. (A–C) The mRNA levels of MMP-2, MMP-9, and TIMP-1 were detected by qPCR in different groups. (D–G) The protein levels of MMP-2, MMP-9, and TIMP-1 were detected by western blotting, and the ratio of MMP-2, MMP-9, and TIMP-1 with β-actin were shown. # P < 0.05 to control, ## P < 0.01 to control, ∗ P < 0.05 to EMS, ∗∗ P < 0.01 to EMS. Columns, mean ( n = 3). Bars, SD. EMS, endometriosis; JFS, Jiawei Foshou San .<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00811/full'>30093862</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9669-3-IHC-anti-mmp-9-antibody.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP-9 using anti-MMP-9 antibody (PB9669). <br> MMP-9 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MMP-9 Antibody (PB9669) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp10-picoband-trade-antibody-pb9670-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9670-1-WB-anti-mmp10-picoband-antibody.jpgAnti-MMP10 Antibody Picoband&reg; Western blot analysis of MMP10 using anti-MMP10 antibody (PB9670). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: SGC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP10 antigen affinity purified polyclonal antibody (Catalog # PB9670) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP10 at approximately 54 kDa. The expected band size for MMP10 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mesothelin-picoband-trade-antibody-pb9671-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9671-1-IHC-anti-mesothelin-picoband-antibody.jpgAnti-Mesothelin/MSLN Antibody IHC analysis of Mesothelin using anti-Mesothelin antibody (PB9671). <br> Mesothelin was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Mesothelin Antibody (PB9671) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ndrg2-picoband-trade-antibody-pb9672-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9672-ndrg2-primary-antibodies-wb-testing-1.jpgAnti-NDRG2 Antibody Picoband&reg;Western blot analysis of NDRG2 using anti-NDRG2 antibody (PB9672). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG2 antigen affinity purified polyclonal antibody (PB9672) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NDRG2 at approximately 41 kDa. The expected band size for NDRG2 is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9672-ndrg2-primary-antibodies-if-testing-1.jpgAnti-NDRG2 Antibody Picoband&reg;IF analysis of NDRG2 using anti-NDRG2 antibody (PB9672). <br> NDRG2 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NDRG2 Antibody (PB9672) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-card12-picoband-trade-antibody-pb9673-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9673-1-WB-anti-card12-picoband-antibody.jpgAnti-CARD12/NLRC4 Antibody Picoband&reg; Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. <br> Lane 1: 22RV1 Whole Cell Lysate,<br> Lane 2: RH35 Whole Cell Lysate,<br> Lane 3: NIH3T3 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fak-picoband-trade-antibody-pb9674-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9674-ptk2-primary-antibodies-wb-testing-1.jpgAnti-FAK/PTK2 Antibody Picoband&reg; Western blot analysis of FAK/PTK2 using anti-FAK/PTK2 antibody (PB9674). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human PC-3 whole cell lysates.<br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human A431 whole cell lysates, <br> Lane 5: human A549 whole cell lysates, <br> Lane 6: human HepG2 whole cell lysates, <br> Lane 7: rat brain tissue lysates, <br> Lane 8: rat C6 whole cell lysates, <br> Lane 9: mouse lung tissue lysates, <br> Lane 10: mouse brain tissue lysates, <br> Lane 11: mouse RAW264.7 whole cell lysates, <br> Lane 12: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK/PTK2 antigen affinity purified polyclonal antibody (Catalog # PB9674) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK/PTK2 at approximately 125 kDa. The expected band size for FAK/PTK2 is at 119 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9674-ptk2-primary-antibodies-ihc-testing-1.jpgAnti-FAK/PTK2 Antibody Picoband&reg;IHC analysis of FAK/PTK2 using anti-FAK/PTK2 antibody (PB9674). <br> FAK/PTK2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FAK/PTK2 Antibody (PB9674) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-shp2-picoband-trade-antibody-pb9675-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9675-ptpn11-primary-antibodies-wb-testing-1.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg;Western blot analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (PB9675). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human CCRF-CEM whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse 3T3-L1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2/PTPN11 antigen affinity purified polyclonal antibody (Catalog # PB9675) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHP2/PTPN11 at approximately 68 kDa. The expected band size for SHP2/PTPN11 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9675-ptpn11-primary-antibodies-ihc-testing-1.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg;IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (PB9675). <br> SHP2/PTPN11 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHP2/PTPN11 Antibody (PB9675) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9675-ptpn11-primary-antibodies-ihc-testing-2.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg;IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (PB9675). <br> SHP2/PTPN11 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHP2/PTPN11 Antibody (PB9675) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9675-ptpn11-primary-antibodies-ihc-testing-3.jpgAnti-SHP2/PTPN11 Antibody Picoband&reg;IHC analysis of SHP2/PTPN11 using anti-SHP2/PTPN11 antibody (PB9675). <br> SHP2/PTPN11 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SHP2/PTPN11 Antibody (PB9675) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s100-alpha-6-picoband-trade-antibody-pb9676-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9676-s100a6-primary-antibodies-wb-testing-1.jpgAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; Western blot analysis of S100A6 using anti-S100A6 antibody (PB9676). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SW620 whole cell lysates,<br> Lane 3: human placenta tissue lysates,<br> Lane 4: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A6 antigen affinity purified polyclonal antibody (Catalog # PB9676) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A6 at approximately 10 kDa. The expected band size for S100A6 is at 10 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9676-2-IHC-anti-s100-alpha-6-picoband-antibody.jpgAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; IHC analysis of S100A6 using anti-S100A6 antibody (PB9676). <br> S100A6 was detected in paraffin-embedded section of Mouse Testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-S100A6 Antibody (PB9676) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9676-3-IHC-anti-s100-alpha-6-picoband-antibody.jpgAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; IHC analysis of S100A6 using anti-S100A6 antibody (PB9676). <br> S100A6 was detected in paraffin-embedded section of Rat Lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-S100A6 Antibody (PB9676) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9676-4-IHC-anti-s100-alpha-6-picoband-antibody.jpgAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; IHC analysis of S100A6 using anti-S100A6 antibody (PB9676). <br> S100A6 was detected in paraffin-embedded section of Human Mammary Cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-S100A6 Antibody (PB9676) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9676-5-IHC-anti-s100-alpha-6-picoband-antibody.jpgAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; IHC analysis of S100A6 using anti-S100A6 antibody (PB9676). <br> S100A6 was detected in paraffin-embedded section of Human Placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-S100A6 Antibody (PB9676) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9676-s100a6-primary-antibodies-if-testing-6.jpgAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; IF analysis of S100A6 using anti-S100A6 antibody (PB9677).<br> S100A6 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-S100A6 Antibody (PB9677) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The tissue section was developed using Phalloidin-iFluor 594 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9676-7.pngAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-S100A6 antibody (PB9676). <br> Overlay histogram showing SiHa cells stained with PB9676 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A6 Antibody (PB9676&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9676-8.pngAnti-S100 alpha 6/S100A6 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-S100A6 antibody (PB9676). <br> Overlay histogram showing A431 cells stained with PB9676 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A6 Antibody (PB9676&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s100a9-picoband-trade-antibody-pb9677-boster.html2026-03-25T05:22:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9677-s100a9-primary-antibodies-wb-testing-1.jpgAnti-S100A9 Antibody Picoband&reg; Western blot analysis of S100A9 using anti-S100A9 antibody (PB9677). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (PB9677) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13 kDa. The expected band size for S100A9 is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9677-2-IHC-anti-s100a9-picoband-antibody.jpgAnti-S100A9 Antibody Picoband&reg; IHC analysis of S100A9 using anti-S100A9 antibody (PB9677). <br> S100A9 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A9 Antibody (PB9677) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9677-3-IHC-anti-s100a9-picoband-antibody.jpgAnti-S100A9 Antibody Picoband&reg; IHC analysis of S100A9 using anti-S100A9 antibody (PB9677). <br> S100A9 was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A9 Antibody (PB9677) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9677-s100a9-primary-antibodies-if-testing-4.jpg.jpgAnti-S100A9 Antibody Picoband&reg; IF analysis of S100A9 using anti-S100A9 antibody (PB9677). <br> S100A9 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-S100A9 Antibody (PB9677) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9677-s100a9-primary-antibodies-fcm-testing-5.jpg.pngAnti-S100A9 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-S100A9 antibody (PB9677). <br>Overlay histogram showing A431 cells stained with PB9677 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A9 Antibody (PB9677, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s100a9-picoband-trade-antibody-pb9678-boster.html2026-04-01T05:01:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9678-s100a9-primary-antibodies-wb-testing-1.jpgAnti-S100A9 Antibody Picoband&reg; Western blot analysis of S100A9 using anti-S100A9 antibody (PB9678). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates, <br> Lane 2: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody (Catalog # PB9678) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A9 at approximately 13-15 kDa. The expected band size for S100A9 is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9678-12964_2025_2141_fig5_html.pngAnti-S100A9 Antibody Picoband&reg;Neutrophils are involved in the severe inflammation in the lung tissue of Stx12 −/− mice. A KEGG pathway analysis of the differentially upregulated expressed genes in Stx12 −/− mice (-log 10 (padj) > 5.5) from the RNA-seq data. B The number of neutrophils in the blood from Stx12 −/− and the littermate control (WT, Stx12 +/+ ) mice. C The relative mRNA levels of neutrophil markers (Ly6g, MPO, ELANE, and CSF3R) and key chemokines for neutrophil recruitment and activation (CSF3, C3, CXCL1, CXCL2, CXCL5, and TNF) normalized to those of β-actin. WT n = 5, HO n = 5 independent samples. D Representative confocal images of immunofluorescence of frozen lung slice using antibodies against S100A9 and ly6g, the markers of neutrophils. Their increase of fluorescence intensity indicates the infiltration of neutrophils (WT n = 4; KO n = 3). E , F Quantification of the total fluorescence intensity of S100A9 and ly6g. It is relative to normalized WT intensity. G , H Quantification of fluorescence intensity of S100A9 and ly6g per cell. It is relative to normalized WT intensity. I Western blot analysis of the expression of IL-6, S100A9 and GAPDH in lung lysates from E19.5 Stx12 −/− (HO) and the littermate control (WT, Stx12 +/+ ) mice. J Relative quantification of IL-6 and S100A9 levels normalized to GAPDH. K IL-1β levels in the lung lysate from Stx12 − / − ( n = 8) and the littermate control (WT) mice ( n = 6) at E19.5 by enzyme-linked immunosorbent assay. L Relative mRNA levels of IL-6 from lung lysates of Stx12 −/− (HO) and the littermate control (WT) mice. M Heatmap showing upregulated inflammation-related genes including neutrophil-related gene, interleukins, tumor necrosis factor (TNF)-related genes, complement(C)-related genes, colony stimulating factor (CSF)-related genes and chemokines in Stx12 −/− (HO) and the littermate control (WT) mice lung tissues. The values are represented as log2 fold changes. The Stx12 −/− mice and their littermate controls were obtained at embryonic day 19.5 (E19.5). The results are presented as the mean ± SEM; statistical significance was assessed by Student’s t- test <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12964-025-02141-y'>40200300</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9678-oncotarget-08-53465-g007.jpgAnti-S100A9 Antibody Picoband&reg;Validation of differential expression mRNAs and lncRNAs in spinal cord by real time RT-qPCR and Western blot analysis. (A) The differential expression mRNA levels were validated by qRT-PCR. (B) Five upregulated lncRNAs and five downregulated lncRNAs were validated by qRT-PCR. The levels of mRNAs and lncRNAs were normalized to GAPDH and expressed as fold of change compared to sham group. The results represent the mean± SEM of three independent experiments. *p < 0.05; **p < 0.01 compared with the sham group. Western blot analysis shown protein expression levels of Akt, P-Akt (C) , Bcl-2, Caspase-3 (D) , P2×7R, S100A9, Bax (E) . Each bar represents the mean ± SEM for at least 6 animals. *p< 0.05, **p< 0.01 vs Sham group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5581123/&orig_host=www.ncbi.nlm.nih.gov'>28881824</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9678-2-IHC-anti-s100a9-picoband-antibody.jpgAnti-S100A9 Antibody Picoband&reg; IHC analysis of S100A9 using anti-S100A9 antibody (PB9678). <br> S100A9 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A9 Antibody (PB9678) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9678-3-IHC-anti-s100a9-picoband-antibody.jpgAnti-S100A9 Antibody Picoband&reg; IHC analysis of S100A9 using anti-S100A9 antibody (PB9678). <br> S100A9 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A9 Antibody (PB9678) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9678-s100a9-primary-antibodies-if-testing-4.jpgAnti-S100A9 Antibody Picoband&reg; IF analysis of S100A9 using anti-S100A9 antibody (PB9678). <br> S100A9 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-S100A9 Antibody (PB9678) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9678-s100a9-primary-antibodies-fcm-testing-5.pngAnti-S100A9 Antibody Picoband&reg; Flow Cytometry analysis of HEPA1-6 cells using anti-S100A9 antibody (PB9678). <br>Overlay histogram showing HEPA1-6 cells stained with PB9678 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A9 Antibody (PB9678, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sectm1-picoband-trade-antibody-pb9679-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9679-1-WB-anti-sectm1-picoband-antibody.jpgAnti-SECTM1/Sectm1b Antibody Picoband&reg; Western blot analysis of SECTM1 using anti-SECTM1 antibody (PB9679). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HEPA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SECTM1 antigen affinity purified polyclonal antibody (Catalog # PB9679) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SECTM1 at approximately 23 kDa. The expected band size for SECTM1 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xcl1-lymphotactin-picoband-trade-antibody-pb9681-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9681-1-WB-anti-xcl1-lymphotactin-antibody.jpgAnti-Lymphotactin/XCL1 Antibody Picoband&reg; Western blot analysis of Lymphotactin using anti-Lymphotactin antibody (PB9681). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody (Catalog # PB9681) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 13 kDa. The expected band size for Lymphotactin is at 13 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9681-xcl1-primary-antibodies-elisa-testing-2.jpgAnti-Lymphotactin/XCL1 Antibody Picoband&reg; Sandwich ELISA - Recombinant human Lymphotactin/XCL1 protein standard curve.<br> Use in combination with reagents from Human Lymphotactin/XCL1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0802). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp12-antibody-rp1089-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1089-1_1.jpgAnti-Macrophage metalloelastase MMP12 Antibody Picoband&reg;Anti-RP1089 antibody&#44; PBMMP12&#44; Western blotting<br>All lanes: Anti MMP12 (RP1089) at 0.5ug/ml<br>WB: HEPA Whole Cell Lysate at 40ug<br>Predicted bind size: 55KD<br>Observed bind size: 55KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nm23a-antibody-rp1090-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1090-nm23a-primary-antibodies-wb-testing-1.jpgAnti-NM23A/NME1 Antibody Picoband&reg; Western blot analysis of NM23A/NME1 using anti-NM23A/NME1 antibody (RP1090). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: human MCF-7 whole cell lysates,<br> Lane 6: human A375 whole cell lysates,<br> Lane 7: human MOLT-4 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A/NME1 antigen affinity purified polyclonal antibody (Catalog # RP1090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A/NME1 at approximately 17 kDa. The expected band size for NM23A/NME1 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1090-nm23a-primary-antibodies-wb-testing-2.jpgAnti-NM23A/NME1 Antibody Picoband&reg; Western blot analysis of NM23A/NME1 using anti-NM23A/NME1 antibody (RP1090). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates,<br> Lane 7: mouse lung tissue lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NM23A/NME1 antigen affinity purified polyclonal antibody (Catalog # RP1090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NM23A/NME1 at approximately 17 kDa. The expected band size for NM23A/NME1 is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1090-nm23a-primary-antibodies-fcm-testing-3.jpgAnti-NM23A/NME1 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-NM23A/NME1 antibody (RP1090). <br> Overlay histogram showing HEL cells stained with RP1090 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NM23A/NME1 Antibody (RP1090, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/picokine-elisa-kits/mouse-hgf-picokine-trade-elisa-kit-ek1217-boster.html2026-03-24T05:05:14+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1217.pngMouse HGF/Hepatocyte growth factor ELISA Kit PicoKine®Mouse HGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lbp-picokine-trade-elisa-kit-ek1271-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1271.jpgHuman LBP ELISA Kit PicoKine®Human LBP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-shbg-picokine-trade-elisa-kit-ek1236-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1236_1.jpgHuman SHBG / Sex Hormone Binding Globulin ELISA Kit PicoKine®Human SHBG PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-mmp-8-picokine-trade-elisa-kit-ek1248-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1248_1.pngRat MMP-8/Neutrophil collagenase ELISA Kit PicoKine®Rat MMP-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-vegf-b-picokine-trade-elisa-kit-ek1411-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1411_1.pngMouse VEGF-B ELISA Kit PicoKine®Mouse VEGF-B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-epcam-picokine-trade-elisa-kit-ek0755-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0755.pngHuman EPCAM/Trop1 ELISA Kit PicoKine®Human EPCAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd5l-picokine-trade-elisa-kit-ek1413-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1413_1.pngHuman CD5L/CT-2 ELISA Kit PicoKine®Human CD5L PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd5l-picokine-trade-elisa-kit-ek1414-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1414_2.pngMouse CD5L/CT-2 ELISA Kit PicoKine®Mouse CD5L PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-cd44-picokine-trade-elisa-kit-ek1417-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1417.jpgRat CD44 ELISA Kit PicoKine®Rat CD44 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd47-picokine-trade-elisa-kit-ek1421-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1421.jpgHuman CD47 ELISA Kit PicoKine®Human CD47 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd73-nt5e-picokine-trade-elisa-kit-ek1422-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1422.pngHuman CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®Human CD73 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grk3-picoband-trade-antibody-pb9682-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9682-1-WB-anti-grk3-picoband-antibody.jpgAnti-GRK3/ADRBK2 Antibody Picoband&reg; Western blot analysis of GRK using anti-GRK antibody (PB9682). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: JURKAT Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK antigen affinity purified polyclonal antibody (Catalog # PB9682) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRK at approximately 80 kDa. The expected band size for GRK is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9682-2.jpgAnti-GRK3/ADRBK2 Antibody Picoband&reg; IF analysis of GRK3 using anti-GRK3 antibody (PB9682). <br> GRK3 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GRK3 Antibody (PB9682) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9682-3.jpgAnti-GRK3/ADRBK2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-GRK3 antibody (PB9682).<br> Overlay histogram showing A431 cells stained with PB9682 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK3 Antibody (PB9682,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aim2-picoband-trade-antibody-pb9683-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9683-aim2-primary-antibodies-wb-testing-1.jpgAnti-AIM2 Antibody Picoband&reg; Western blot analysis of AIM2 using anti-AIM2 antibody (PB9683). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human Daudi whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIM2 antigen affinity purified polyclonal antibody (Catalog # PB9683) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIM2 at approximately 45 kDa. The expected band size for AIM2 is at 39,40-45 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9683-fnmol-12-00036-g004.jpgAnti-AIM2 Antibody Picoband&reg;Gene expression and cellular distribution of NLRP1, NLRP3 and Aim2 sensors in the retina. (A) RNAscope analysis of NLRP1 (green dots, yellow arrows) and NLRP3 (white dots, white arrows) transcript abundance in normotensive control (NT control) and experimental (OHT) retinas at 12 h postinjury. (B) Aim2 transcript (red dots) abundance in normotensive control and experimental retinas. Co-staining for the Muller glia marker glutamine synthetase (GlSyn, green) is added to indicate cellular expression of Aim2 in the GCL layer (yellow arrows) and INL (white arrows) in control and 12 h post-OHT retinas. Bar, 25 μm on all panels.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2019.00036/full'>30930743</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9683-fnmol-12-00036-g005.jpgAnti-AIM2 Antibody Picoband&reg;OHT-induced upregulation of Aim2 inflammasome in the retina. (A) NLRP1 antibody labeling (red) in the inner retina; yellow arrows indicate colocalization with RGC neurons (RBPSM + ) in the GCL. (B) Immunohistochemistry for Aim2 protein (red) and Muller glia marker (GlSyn, green). Arrows show colocalization with Muller glia; no colocalization is detected with astrocytes (GFAP, white) in both control and 24 h post-OHT retinas. (C) NLRP3 labeling (green) in the inner retina of naïve and control eyes localized only to blood capillaries (white arrowhead) and astrocytes (yellow arrows), but not to RGCs (RBPMS cells). At 24 h post-injury the labeling in large cells in the GCL and INL (white arrows) are observed. (D) The NLRP1and NLRP3 proteins localized to distinct cell types in the GCL of naïve and NT control retinas: NLRP3 (green) is only expressed in blood vessels (white arrowheads). Twenty four hours after OHT, NLRP3 colocalizes with NLRP1 in RGCs (yellow arrows) and to RBPMS- cells (white arrows). Blue arrowheads denote labeling in RGC axons and dendrites. Bar, 25 μm on all panels.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/molecular-neuroscience/articles/10.3389/fnmol.2019.00036/full'>30930743</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9683-2-IHC-anti-aim2-picoband-antibody.jpgAnti-AIM2 Antibody Picoband&reg; IHC analysis of AIM2 using anti-AIM2 antibody (PB9683). <br> AIM2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AIM2 Antibody (PB9683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9683-3-IHC-anti-aim2-picoband-antibody.jpgAnti-AIM2 Antibody Picoband&reg; IHC analysis of AIM2 using anti-AIM2 antibody (PB9683). <br> AIM2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AIM2 Antibody (PB9683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9683-4-IHC-anti-aim2-picoband-antibody.jpgAnti-AIM2 Antibody Picoband&reg; IHC analysis of AIM2 using anti-AIM2 antibody (PB9683). <br> AIM2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AIM2 Antibody (PB9683) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fe65-picoband-trade-antibody-pb9684-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9684-1-WB-anti-fe65-picoband-antibody.jpgAnti-FE65/APBB1 Antibody Picoband&reg; Western blot analysis of FE65 using anti-FE65 antibody (PB9684). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: U87 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FE65 antigen affinity purified polyclonal antibody (Catalog # PB9684) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FE65 at approximately 65 kDa. The expected band size for FE65 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9684-2-IHC-anti-fe65-picoband-antibody.jpgAnti-FE65/APBB1 Antibody Picoband&reg; IHC analysis of FE65 using anti-FE65 antibody (PB9684). <br> FE65 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FE65 Antibody (PB9684) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9684-3-IHC-anti-fe65-picoband-antibody.jpgAnti-FE65/APBB1 Antibody Picoband&reg; IHC analysis of FE65 using anti-FE65 antibody (PB9684). <br> FE65 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FE65 Antibody (PB9684) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9684-4-IHC-anti-fe65-picoband-antibody.jpgAnti-FE65/APBB1 Antibody Picoband&reg; IHC analysis of FE65 using anti-FE65 antibody (PB9684). <br> FE65 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FE65 Antibody (PB9684) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arid1a-picoband-trade-antibody-pb9685-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9685-arid1a-primary-antibodies-wb-testing-1.jpgAnti-ARID1A Antibody Picoband&reg;Western blot analysis of ARID1A using anti-ARID1A antibody (PB9685). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human THP-1 whole cell lysates.<br> Lane 3: human Jurkat whole cell lysates.<br> Lane 4: human SH-SY5Y whole cell lysates.<br> Lane 5: mouse testis tissue lysates.<br> Lane 6: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARID1A antigen affinity purified polyclonal antibody (PB9685) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ARID1A at approximately 250-270 kDa. The expected band size for ARID1A is at 242 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9685-arid1a-primary-antibodies-if-testing-1.jpgAnti-ARID1A Antibody Picoband&reg;IF analysis of ARID1A using anti-ARID1A antibody (PB9685). <br> ARID1A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ARID1A Antibody (PB9685) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9685-arid1a-primary-antibodies-fcm-testing-1.jpgAnti-ARID1A Antibody Picoband&reg;Flow Cytometry analysis of Hela cells using anti-ARID1A antibody (PB9685). <br> Overlay histogram showing Hela cells stained with PB9685 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARID1A Antibody (PB9685, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-b3gnt8-picoband-trade-antibody-pb9686-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9686-1-WB-anti-b3gnt8-picoband-antibody.jpgAnti-B3GNT8 Antibody Picoband&reg; Western blot analysis of B3GNT8 using anti-B3GNT8 antibody (PB9686). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B3GNT8 antigen affinity purified polyclonal antibody (Catalog # PB9686) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B3GNT8 at approximately 43 kDa. The expected band size for B3GNT8 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9686-2-IHC-anti-b3gnt8-picoband-antibody.jpgAnti-B3GNT8 Antibody Picoband&reg; IHC analysis of B3GNT8 using anti-B3GNT8 antibody (PB9686). <br> B3GNT8 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-B3GNT8 Antibody (PB9686) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmp-2-picoband-trade-antibody-pb9687-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9687-1-WB-anti-bmp-2-antibody.jpgAnti-BMP2 Antibody Picoband&reg; Western blot analysis of BMP-2 using anti-BMP-2 antibody (PB9687). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Lung Tissue Lysate at 50ug,<br> Lane 2: Rat Brain Tissue Lysate at 50ug,<br> Lane 3: U87 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP-2 antigen affinity purified polyclonal antibody (Catalog # PB9687) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP-2 at approximately 20 kDa, 40 kDa. The expected band size for BMP-2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9687-2-IHC-anti-bmp-2-antibody.jpgAnti-BMP2 Antibody Picoband&reg; IHC analysis of BMP-2 using anti-BMP-2 antibody (PB9687). <br> BMP-2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BMP-2 Antibody (PB9687) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-13036_2023_324_fig4_html.pngAnti-BMP2 Antibody Picoband&reg;Effects of exosomes on the activation of osteoblastic-associated protein expression in HK2 cells. A RT-qPCR analysis of BMP2,OPN and OCN expression in the HK2 cells stimulated with exosomes derived from HK2 cells under different conditions. B Immunofluorescence analysis of OPN and OCN expression in the HK2 cells stimulated with exosomes derived from HK2 cells under different conditions. C Western blot assays showing the expression levels of BMP2, OPN, and OCN in the Hk2 cells stimulated with EXO(C) or EXO(S). The data are expressed as the mean ± SE. ∗ P < 0.05 compared with the HK2 cells <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13036-023-00324-0'>36855143</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-13046_2010_article_332_fig1_html.jpgAnti-BMP2 Antibody Picoband&reg;The mRNA expression of BMP-2 and its receptors detected by RT-PCR 1: Ovarian cancer tissue; 2: Benign ovarian tumor tissue; 3: Normal ovarian tissue; M: Marker . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-29-85'>20587070</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-13046_2010_article_332_fig2_html.jpgAnti-BMP2 Antibody Picoband&reg;The protein expression of BMP-2 and its receptors detected by western blot 1: Ovarian cancer tissue; 2: Benign ovarian tumor tissue; 3: Normal ovarian tissue . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-29-85'>20587070</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-13046_2010_article_332_fig3_html.jpgAnti-BMP2 Antibody Picoband&reg;Expression of BMP-2, BMPRIA, BMPRIB, and BMPRII in epithelial serous ovarian cancer detected by immunohistochemistry (×400) A: BMP-2, B: BMPRIA, C: BMPRIB, D: BMPRII . <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-29-85'>20587070</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-13036_2023_324_fig7_html.pngAnti-BMP2 Antibody Picoband&reg;Effects of exosomes derived from HK2 cells under different conditions on the activation of osteoblastic-associated protein expression in rat kidneys. A BMP2,OPN, and OCN were highly expressed in the rats injected with EXO(S) or GAM, and pre-EXO(C) renal subcapsular injection could ameliorate these effects,while pre-EXO(S) renal subcapsular injection could accelerate the expression of osteoblastic-associated proteins. The expression levels of BMP2,OPN, and OCN were quantified via ImageJ. B The expression of BMP2,OPN and OCN in the rats with different treatments (magnification 200X). Three rats from each group were used for quantification via ImageJ. Three animals from each group were used for these data. The data are expressed as the mean ± SE. ∗ P < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13036-023-00324-0'>36855143</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-mv-v14-2373-f1.jpgAnti-BMP2 Antibody Picoband&reg;Distribution of BMP-2 in human sclera by indirect immunofluorescence. FITC marked the secondary antibody (green; A ) and Hoechst33342 dyed the nucleus (blue; B ). The first ( A ) and second images ( B ) are combined to form the third image shown ( C ). BMP-2 is localized in the cytoplasm of HSF and extracellular matrices ( A - C ). Magnification: 400X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605409/'>19098993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-mv-v14-2373-f2.jpgAnti-BMP2 Antibody Picoband&reg;Distribution of BMP-2 in human sclera fibroblasts in vitro using indirect immunofluorescence. FITC marked the secondary antibody (green; A ), and PI dyed the nucleus (red; B ). The first ( A ) and second images ( B ) are combined to form the third image shown ( C ). BMP-2 is localized in the cytoplasm and weakly in the nucleus of HSF ( A - C ). Magnification: 400X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605409/'>19098993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-mv-v14-2373-f3.jpgAnti-BMP2 Antibody Picoband&reg;The changed pattern of human sclera fibroblasts incubated with rhBMP-2. The HSFs showed only a sparse and vortex pattern before incubation ( A ) whereas incubation with 100 ng/ml rhBMP-2 changed the HSFs into the intensive and polygonal cells ( B ). Inverted phase contrast microscope, original magnification 100X.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605409/'>19098993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-images_medium_s1793545824420021figf6.pngAnti-BMP2 Antibody Picoband&reg;IF staining images of (a) BMP-2, (b) COL-1, (c) OCN, and (d) OPN in BMSCs (red: osteogenic proteins and genes; green: cytoskeleton; blue: nucleus). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.worldscientific.com/doi/abs/10.1142/S1793545824420021'>10.1142/S1793545824420021</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-mv-v14-2373-f4.jpgAnti-BMP2 Antibody Picoband&reg;Time-dependent effects of rhBMP-2 on the cell proliferation of primary cultured human sclera fibroblasts. Cell proliferation was determined by MTT assay. The optical density value at A490 and the mean±SD is provided for each time point (n=8). The cells were incubated with no rhBMP-2 or 100 ng/ml rhBMP-2 for seven days. A significant difference was seen in cell proliferation between 100 ng/ml rhBMP-2 treatment and control after four days. The asterisk indicates that p<0.05 when compared with the control without rhBMP-2.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605409/'>19098993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-mv-v14-2373-f5.jpgAnti-BMP2 Antibody Picoband&reg;Effect of rhBMP-2 on mRNA expression levels of MMP-2 and TIMP-2 in human sclera fibroblasts. Ethidium-bromide agarose gels indicated the level of β-actin message relative to MMP-2 ( A ) and TIMP-2 ( B ) levels from total RNA. Bar graphs revealed changes in mRNA expression (mean±standard error of the mean) where values were normalized to β-actin values and expression was stated as a ratio of optical density. The double asterisk means that the semi-quantitative RT-PCR revealed significant changes for MMP-2 and TIMP-2 with varying concentrations of rhBMP-2 (p<0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605409/'>19098993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-mv-v14-2373-f6.jpgAnti-BMP2 Antibody Picoband&reg;Effect of BMP-2 on TIMP-2 protein secreted from human sclera fibroblasts into culture medium. Cells were treated with 0, 1, 10, and 100 ng/ml rhBMP-2 for 12, 24, and 48 h. There were no significant differences in secreted protein levels of TIMP-2 in the HSF incubated with different rhBMP-2 concentrations after 24 h. The TIMP-2 protein levels were significantly increased following treatment with 100 ng/ml rhBMP-2 after 48 h (p< 0.01) and 10 ng/ml rhBMP-2 after 48 h (P < 0.05). Data were represented as the mean±SEM. Data were from three independent experiments. Each sample was assayed in duplicate. An asterisk indicates a p<0.05 compared to control values, and a double asterisk indicates a p< 0.01 compared to control values.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605409/'>19098993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-mv-v14-2373-f7.jpgAnti-BMP2 Antibody Picoband&reg;Effect of BMP-2 on MMP-2 protein secretion from human sclera fibroblasts into culture medium. Cells were treated with 0, 1, 10, and 100 ng/ml rhBMP-2 for 12, 24, and 48 h. There were no significant differences in secreted protein levels of MMP-2 in the HSF incubated with different rhBMP-2 concentrations after 24 h. The MMP-2 protein levels were significantly decreased following treatment of HSF cells with 100 ng/ml rhBMP-2 after 48 h (p<0.05). Data were represented as the mean±SEM. Data were from three independent experiments. Each sample was assayed in duplicate. An asterisk indicates a p<0.05 compared to control values.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2605409/'>19098993</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9687-bmp2-primary-antibodies-elisa-testing-3.jpgAnti-BMP2 Antibody Picoband&reg; Sandwich ELISA - Recombinant mouse BMP2 protein standard curve.<br> Use in combination with reagents from Mouse BMP2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0313). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmp-4-picoband-trade-antibody-pb9688-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9688-1-WB-anti-bmp-4-antibody.jpgAnti-BMP4 Antibody Picoband&reg; Western blot analysis of BMP-4 using anti-BMP-4 antibody (PB9688). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Mouse Lung Tissue Lysate at 50ug,<br> Lane 2: Mouse Liver Tissue Lysate at 50ug,<br> Lane 3: HEPA Whole Cell Lysate at 40ug,<br> Lane 4: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 5: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP-4 antigen affinity purified polyclonal antibody (Catalog # PB9688) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP-4 at approximately 46 kDa. The expected band size for BMP-4 is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmp-5-picoband-trade-antibody-pb9689-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9689-1-WB-anti-bmp-5-antibody.jpgAnti-BMP5 Antibody Picoband&reg; Western blot analysis of BMP-5 using anti-BMP-5 antibody (PB9689). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Mouse Liver Tissue Lysate at 50ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP-5 antigen affinity purified polyclonal antibody (Catalog # PB9689) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP-5 at approximately 51 kDa. The expected band size for BMP-5 is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9689-2-IHC-anti-bmp-5-antibody.jpgAnti-BMP5 Antibody Picoband&reg; IHC analysis of BMP-5 using anti-BMP-5 antibody (PB9689). <br> BMP-5 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BMP-5 Antibody (PB9689) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9689-3-IHC-anti-bmp-5-antibody.jpgAnti-BMP5 Antibody Picoband&reg; IHC analysis of BMP-5 using anti-BMP-5 antibody (PB9689). <br> BMP-5 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BMP-5 Antibody (PB9689) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9689-4-IHC-anti-bmp-5-antibody.jpgAnti-BMP5 Antibody Picoband&reg; IHC analysis of BMP-5 using anti-BMP-5 antibody (PB9689). <br> BMP-5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BMP-5 Antibody (PB9689) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9689-bmp5-primary-antibodies-fc-testing-5.jpgAnti-BMP5 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-BMP5 antibody (PB9689).<br>Overlay histogram showing U20S cells stained with PB9689 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BMP5 Antibody (PB9689&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmp6-picoband-trade-antibody-pb9690-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9690-1-WB-anti-bmp6-picoband-antibody.jpgAnti-BMP6 Antibody Picoband&reg; Western blot analysis of BMP6 using anti-BMP6 antibody (PB9690). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP6 antigen affinity purified polyclonal antibody (Catalog # PB9690) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP6 at approximately 43 kDa. The expected band size for BMP6 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9690-bmp6-primary-antibodies-fc-testing-2.jpgAnti-BMP6 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-BMP6 antibody (PB9690).<br>Overlay histogram showing Hela cells stained with PB9690 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BMP6 Antibody (PB9690&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd22-picoband-trade-antibody-pb9691-boster.html2026-03-24T05:05:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9691-2-IHC-anti-cd22-siglec-2-picoband-antibody.jpgAnti-CD22 Antibody Picoband&reg; IHC analysis of CD22 using anti-CD22 antibody (PB9691). <br> CD22 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD22 Antibody (PB9691) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9691-cd22-primary-antibodies-wb-testing-1.jpgAnti-CD22 Antibody Picoband&reg; Western blot analysis of CD22 using anti-CD22 antibody (PB9691). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Daudi whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human Ramos whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD22 antigen affinity purified polyclonal antibody (Catalog # PB9691) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD22 at approximately 140kDa. The expected band size for CD22 is at 95 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9691-cd22-primary-antibodies-fc-testing-3.pngAnti-CD22 Antibody Picoband&reg; Flow Cytometry analysis of Raji cells using anti-CD22 antibody (PB9691).<br> Overlay histogram showing Raji cells stained with PB9691 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD22 Antibody (PB9691&#44;1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-chk2-picoband-trade-antibody-pb9692-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9692-1-WB-anti-chk2-picoband-antibody.jpgAnti-Chk2/CHEK2 Antibody Picoband&reg; Western blot analysis of Chk2 using anti-Chk2 antibody (PB9692). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Spleen Tissue Lysate at 50ug,<br> Lane 2: Mouse Testis Tissue Lysate at 50ug,<br> Lane 3: SW620 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chk2 antigen affinity purified polyclonal antibody (Catalog # PB9692) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chk2 at approximately 65 kDa. The expected band size for Chk2 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9692-2-IHC-anti-chk2-picoband-antibody.jpgAnti-Chk2/CHEK2 Antibody Picoband&reg; IHC analysis of Chk2 using anti-Chk2 antibody (PB9692). <br> Chk2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Chk2 Antibody (PB9692) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-csnk1a1-picoband-trade-antibody-pb9693-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9693-1-WB-anti-csnk1a1-casein-kinase-1-alpha-picoband-antibody.jpgAnti-Caseine Kinase 1 alpha/CSNK1A1 Antibody Picoband&reg; Western blot analysis of CSNK1A1 using anti-CSNK1A1 antibody (PB9693). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44; <br> Lane 2: Rat Kidney Tissue Lysate&#44; <br> Lane 3: Mouse Kidney Tissue Lysate&#44; <br> Lane 4: HELA Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK1A1 antigen affinity purified polyclonal antibody (Catalog # PB9693) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK1A1 at approximately 39KD. The expected band size for CSNK1A1 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9693-2-IHC-anti-csnk1a1-casein-kinase-1-alpha-picoband-antibody.jpgAnti-Caseine Kinase 1 alpha/CSNK1A1 Antibody Picoband&reg; IHC analysis of CSNK1A1 using anti-CSNK1A1 antibody (PB9693).<br> CSNK1A1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CSNK1A1 Antibody (PB9693) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9693-3-IHC-anti-csnk1a1-casein-kinase-1-alpha-picoband-antibody.jpgAnti-Caseine Kinase 1 alpha/CSNK1A1 Antibody Picoband&reg; IHC analysis of CSNK1A1 using anti-CSNK1A1 antibody (PB9693).<br> CSNK1A1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CSNK1A1 Antibody (PB9693) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9693-4-IHC-anti-csnk1a1-casein-kinase-1-alpha-picoband-antibody.jpgAnti-Caseine Kinase 1 alpha/CSNK1A1 Antibody Picoband&reg; IHC analysis of CSNK1A1 using anti-CSNK1A1 antibody (PB9693).<br> CSNK1A1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CSNK1A1 Antibody (PB9693) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcl9-picoband-trade-antibody-pb9694-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9694-1-WB-anti-cxcl9-mig-antibody.jpgAnti-MIG/CXCL9 Antibody Picoband&reg; Western blot analysis of CXCL9 using anti-CXCL9 antibody (PB9694). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Human Placenta Tissue Lysate at 50ug,<br> Lane 2: A431 Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL9 antigen affinity purified polyclonal antibody (Catalog # PB9694) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL9 at approximately 19 kDa. The expected band size for CXCL9 is at 14 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ip-10-cxcl10-picoband-trade-antibody-pb9695-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9695-1-WB-anti-cxcl10-ip-10-antibody.jpgAnti-IP10/CXCL10 Antibody Picoband&reg; Western blot analysis of IP10 using anti-IP10 antibody (PB9695). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IP10 antigen affinity purified polyclonal antibody (Catalog # PB9695) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IP10 at approximately 34 kDa. The expected band size for IP10 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ip-10-cxcl10-picoband-trade-antibody-pb9696-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9696-cxcl10-primary-antibodies-wb-testing-1.jpgAnti-IP10/CXCL10 Antibody Picoband&reg; Western blot analysis of CXCL10 using anti-CXCL10 antibody (PB9696). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: recombinant mouse CXCL10 protein 10 ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL10 antigen affinity purified polyclonal antibody (Catalog # PB9696) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL10 at approximately 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9696-cxcl10-primary-antibodies-ihc-testing-2.jpgAnti-IP10/CXCL10 Antibody Picoband&reg; IHC analysis of CXCL10 using anti-CXCL10 antibody (PB9696). <br> CXCL10 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL10 Antibody (PB9696) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9696-cxcl10-primary-antibodies-ihc-testing-3.jpgAnti-IP10/CXCL10 Antibody Picoband&reg;IHC analysis of CXCL10 using anti-CXCL10 antibody (PB9696). <br> CXCL10 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CXCL10 Antibody (PB9696) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bca1-picoband-trade-antibody-pb9697-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9697-1-WB-anti-cxcl13-blc-antibody.jpgAnti-BCA1/CXCL13 Antibody Picoband&reg; Western blot analysis of CXCL13 using anti-CXCL13 antibody (PB9697). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane : HELA whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL13 antigen affinity purified polyclonal antibody (Catalog # PB9697) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL13 at approximately 13KD. The expected band size for CXCL13 is at 13KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-elavl4-picoband-trade-antibody-pb9698-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9698-1-WB-anti-elavl4-picoband-antibody.jpgAnti-ELAVL4 Antibody Picoband&reg; Western blot analysis of ELAVL4 using anti-ELAVL4 antibody (PB9698). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates&#44; <br> Lane 3: U87-MG whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL4 antigen affinity purified polyclonal antibody (Catalog # PB9698) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL4 at approximately 42KD. The expected band size for ELAVL4 is at 42KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9698-2-IHC-anti-elavl4-picoband-antibody.jpgAnti-ELAVL4 Antibody Picoband&reg; IHC analysis of ELAVL4 using anti-ELAVL4 antibody (PB9698). <br> ELAVL4 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ELAVL4 Antibody (PB9698) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogenhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9698-3-IHC-anti-elavl4-picoband-antibody.jpgAnti-ELAVL4 Antibody Picoband&reg; IHC analysis of ELAVL4 using anti-ELAVL4 antibody (PB9698). <br> ELAVL4 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ELAVL4 Antibody (PB9698) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogenhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9698-4-IHC-anti-elavl4-picoband-antibody.jpgAnti-ELAVL4 Antibody Picoband&reg; IHC analysis of ELAVL4 using anti-ELAVL4 antibody (PB9698). <br> ELAVL4 was detected in paraffin-embedded section of human meningeoma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ELAVL4 Antibody (PB9698) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9698-5.jpgAnti-ELAVL4 Antibody Picoband&reg; IF analysis of ELAVL4 using anti-ELAVL4 antibody (PB9698) <br> ELAVL4 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-ELAVL4 Antibody (PB9698) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pea3-picoband-trade-antibody-pb9699-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9699-1-WB-anti-pea3-picoband-antibody.jpgAnti-Pea3/ETV4 Antibody Picoband&reg; Western blot analysis of Pea3 using anti-Pea3 antibody (PB9699). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Lung Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: A549 Whole Cell Lysate at 40ug,<br> Lane 4: SW620 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pea3 antigen affinity purified polyclonal antibody (Catalog # PB9699) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pea3 at approximately 68 kDa. The expected band size for Pea3 is at 54 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-par2-picoband-trade-antibody-pb9700-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9700-1-WB-anti-par2-picoband-antibody.jpgAnti-PAR2/F2RL1 Antibody Picoband&reg; Western blot analysis of PAR2 using anti-PAR2 antibody (PB9700). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. <br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: COLO320 Whole Cell Lysate,<br> Lane 3: SW620 Whole Cell Lysate,<br> Lane 4: HEPG2 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAR2 antigen affinity purified polyclonal antibody (Catalog # PB9700) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAR2 at approximately 49 kDa. The expected band size for PAR2 is at 44 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tissue-factor-picoband-trade-antibody-pb9701-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9701-tissue-factor-primary-antibodies-wb-testing-1.jpgAnti-Tissue Factor/F3 Antibody Picoband&reg; Western blot analysis of Tissue Factor using anti-Tissue Factor antibody (PB9701). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human Hacat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tissue Factor antigen affinity purified polyclonal antibody (Catalog # PB9701) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tissue Factor at approximately 50 kDa. The expected band size for Tissue Factor is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9701-f3-primary-antibodies-elisa-testing-4.jpgAnti-Tissue Factor/F3 Antibody Picoband&reg; Sandwich ELISA - Recombinant human Tissue Factor/F3 protein standard curve.<br> Use in combination with reagents from Human Tissue Factor/F3 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0928).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9701-tissue-factor-primary-antibodies-fcm-testing-4.jpgAnti-Tissue Factor/F3 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-Tissue Factor antibody (PB9701). <br>Overlay histogram showing A431 cells stained with PB9701 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tissue Factor Antibody (PB9701, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9701-tissue-factor-primary-antibodies-ihc-testing-2.jpgAnti-Tissue Factor/F3 Antibody Picoband&reg; IHC analysis of Tissue Factor using anti-Tissue Factor antibody (PB9701). <br> Tissue Factor was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Tissue Factor Antibody (PB9701) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9701-tissue-factor-primary-antibodies-if-testing-3.jpgAnti-Tissue Factor/F3 Antibody Picoband&reg; IF analysis of Tissue Factor using anti-Tissue Factor antibody (PB9701). <br> Tissue Factor was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Tissue Factor Antibody (PB9701) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9701-f3-primary-antibodies-elisa-testing-5.jpgAnti-Tissue Factor/F3 Antibody Picoband&reg; Sandwich ELISA - Recombinant human Tissue Factor/F3 protein standard curve.<br> Use in combination with reagents from Human Tissue Factor/F3 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0928). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tissue-factor-picoband-trade-antibody-pb9702-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9702-1-WB-anti-tissue-factor-f3-antibody.jpgAnti-Tissue Factor/F3 Antibody Picoband&reg; Western blot analysis of Tissue Factor using anti-Tissue Factor antibody (PB9702). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Mouse Lung Tissue Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tissue Factor antigen affinity purified polyclonal antibody (Catalog # PB9702) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tissue Factor at approximately 50 kDa. The expected band size for Tissue Factor is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fmrp-picoband-trade-antibody-pb9703-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9703-fmrp-primary-antibodies-wb-testing-1.jpgAnti-FMRP/FMR1 Antibody Picoband&reg; Western blot analysis of FMRP/FMR1 using anti-FMRP/FMR1 antibody (PB9703). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMRP/FMR1 antigen affinity purified polyclonal antibody (Catalog # PB9703) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMRP/FMR1 at approximately 71-73 kDa. The expected band size for FMRP/FMR1 is at 71 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9703-fmrp-primary-antibodies-ihc-testing-2.jpgAnti-FMRP/FMR1 Antibody Picoband&reg; IHC analysis of FMRP/FMR1 using anti-FMRP/FMR1 antibody (PB9703). <br> FMRP/FMR1 was detected in a paraffin-embedded section of human seminoma testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FMRP/FMR1 Antibody (PB9703) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9703-fmrp-primary-antibodies-if-testing-3.jpgAnti-FMRP/FMR1 Antibody Picoband&reg; IF analysis of FMRP/FMR1 using anti-FMRP/FMR1 antibody (PB9703). <br> FMRP/FMR1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FMRP/FMR1 Antibody (PB9703) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fxyd1-picoband-trade-antibody-pb9704-boster.html2026-04-01T05:01:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9704-1-WB-anti-fxyd1-phospholemman-picoband-antibody.jpgAnti-FXYD1 Antibody Picoband&reg; Western blot analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: Rat Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 3: Mouse Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 4: HEPG2 Whole Cell Lysate at 40ug,<br> Lane 5: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXYZ1 antigen affinity purified polyclonal antibody (Catalog # PB9704) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXYZ1 at approximately 10 kDa. The expected band size for FXYZ1 is at 10 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9704-2-IHC-anti-fxyd1-phospholemman-picoband-antibody.jpgAnti-FXYD1 Antibody Picoband&reg; IHC analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704). <br> FXYZ1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FXYZ1 Antibody (PB9704) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9704-3-IHC-anti-fxyd1-phospholemman-picoband-antibody.jpgAnti-FXYD1 Antibody Picoband&reg; IHC analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704). <br> FXYZ1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FXYZ1 Antibody (PB9704) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9704-4-IHC-anti-fxyd1-phospholemman-picoband-antibody.jpgAnti-FXYD1 Antibody Picoband&reg; IHC analysis of FXYZ1 using anti-FXYZ1 antibody (PB9704). <br> FXYZ1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FXYZ1 Antibody (PB9704) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glp1-picoband-trade-antibody-pb9705-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9705-1-IHC-anti-glp1-picoband-antibody.jpgAnti-Glucagon/GCG Antibody IHC analysis of GLP1 using anti-GLP1 antibody (PB9705). <br> GLP1 was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9705-41598_2016_article_bfsrep34284_fig3_html.jpgAnti-Glucagon/GCG AntibodyExpression of GLP-1 and GIP in ileum. ( A ) Images (×400) of immunofluorescence staining of GLP-1 (glucagon-like peptide-1) and GIP (gastric inhibitory polypeptide) expression in ileum in the Control, DM and DMC groups. ( B ) Immunofluorescence results (×400) indicating GIP expression. (scale bar: 20μm). ( C ) Quantification of the GLP-1 fluorescence intensity (integrated density per stained area). ( D ) Quantification of the GIP fluorescence intensity (integrated density per stained area). *p < 0.05 vs Control, # p < 0.05 vs DM; mean ± SEM. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep34284'>27721485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9705-41598_2016_article_bfsrep34284_fig4_html.jpgAnti-Glucagon/GCG AntibodyGLP-1 and GIP concentrations in the serum and mRNA levels of GCG and GIP. ( A ) GLP-1 concentrations in the serum in the in the Control, DM and DMC groups. ( B ) GCG (the gene encoding GLP-1) mRNA levels in ileum. ( C ) GIP concentrations in the serum in the five groups. ( D ) GIP mRNA levels in ileum. ( E ) Insulin levels in the serum in the five groups. *p < 0.05, **p < 0.01 vs Control, # p < 0.05, ## p < 0.01 vs DM; mean ± SEM. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep34284'>27721485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9705-12986_2010_article_257_fig9_html.jpgAnti-Glucagon/GCG AntibodyHE Staining (×200 magnification) and glucagon staining (×400 magnification) in rat ileum . Ileal villus observed in the CUGFR0 (A1) and CUGFR2 (A2) groups with HE staining and NC4 (B1) and CUGFR4 (B2) groups with glucagon staining. Arrow shows a glucagon-positive cell. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1743-7075-7-45'>20504302</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9705-41598_2016_article_bfsrep34284_fig7_html.jpgAnti-Glucagon/GCG AntibodyAlterations of the protein levels of the components of the GSK-3β—β-catenin—TCF7L2—GLP-1 axis under varying glucose conditions. ( A ) Molecular structure of Emodin. ( B ) Relative protein levels of p-GSK-3β, GSK-3β, β-catenin and TCF7L2 in Control and Emodin groups under different glucose conditions, detected by the western blot analysis. ( C ) Relative mRNA expression of β-catenin and TCF7L2 in Control and Emodin groups under different glucose conditions, detected by real-time PCR. ( D ) GLP-1 and GIP concentration measured by ELISA in the cell supernatant in Control and Emodin groups under different glucose conditions. *p < 0.05, **p < 0.01 vs control under no glucose, # p < 0.05, ## p < 0.01 vs control under low glucose, &p < 0.05, && p < 0.01 vs control under high glucose; mean ± SEM. ( E,F ) Inhibition of TCF7L2 and GSK-3β abolished DMC’s effects. ( E ) Effects of emodin (the main component of DMC) and SB216763 (a specific GSK-3β inhibitor) on the protein levels of β-catenin and TCF7L2 under HG (high glucose) conditions. ( F ) Relative mRNA expression of β-catenin and TCF7L2 in the three groups. ( G ) Relative protein expression of TCF7L2 in HG, HG + emodin and HG + emodin + TCF7L2 SiRNA groups. ( H ) GLP-1 and GIP concentration measured by ELISA in the cell supernatant in the three groups. *p < 0.05, **p < 0.01 vs HG, #p < 0.05, ##p < 0.01 vs HG + Emodin; mean ± SEM. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep34284'>27721485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9705-12986_2010_article_257_fig10_html.jpgAnti-Glucagon/GCG AntibodyEffect of catch-up growth on glucagon-positive cells per high-power field . To quantify the expression of glucagon, an average of 50 sections of rat ileum from each of two animals were examined. The number of cells immunoreactive for glucagon was determined. Data are expressed as means ± SD. *P ≤ 0.05 versus NC group; **P ≤ 0.01 versus NC group. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1743-7075-7-45'>20504302</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9705-2-IHC-anti-glp1-picoband-antibody.jpgAnti-Glucagon/GCG Antibody IHC analysis of GLP1 using anti-GLP1 antibody (PB9705). <br> GLP1 was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9705-3-IHC-anti-glp1-picoband-antibody.jpgAnti-Glucagon/GCG Antibody IHC analysis of GLP1 using anti-GLP1 antibody (PB9705). <br> GLP1 was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLP1 Antibody (PB9705) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9705-4_1.jpgAnti-Glucagon/GCG AntibodyIF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB9705) and anti-Insulin antibody (MA1052).<br>Glucagon/GCG was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Glucagon/GCG Antibody (PB9705) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9705-5_1.jpgAnti-Glucagon/GCG Antibody IF analysis of Glucagon/GCG using anti-Glucagon/GCG antibody (PB9705) and anti-Insulin antibody (MA1052).<br> Glucagon/GCG was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Glucagon/GCG Antibody (PB9705) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gjc2-picoband-trade-antibody-pb9706-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9706-1-WB-anti-gjc2-connexin-47-picoband-antibody.jpgAnti-GJC2 Antibody Picoband&reg; Western blot analysis of GJC2 using anti-GJC2 antibody (PB9706). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: U20S Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJC2 antigen affinity purified polyclonal antibody (Catalog # PB9706) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJC2 at approximately 47 kDa. The expected band size for GJC2 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gnaq-picoband-trade-antibody-pb9707-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9707-c1qbp-primary-antibodies-wb-testing-1.jpgAnti-GNAQ Antibody Picoband&reg; Western blot analysis of C1QBP using anti-C1QBP antibody (PB9707). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1QBP antigen affinity purified polyclonal antibody (Catalog # PB9707) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1QBP at approximately 42 kDa. The expected band size for C1QBP is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9707-2-IHC-anti-gnaq-picoband-antibody.jpgAnti-GNAQ Antibody Picoband&reg; IHC analysis of GNAQ using anti-GNAQ antibody (PB9707). <br> GNAQ was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GNAQ Antibody (PB9707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9707-3-IHC-anti-gnaq-picoband-antibody.jpgAnti-GNAQ Antibody Picoband&reg; IHC analysis of GNAQ using anti-GNAQ antibody (PB9707). <br> GNAQ was detected in a paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GNAQ Antibody (PB9707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9707-4-IHC-anti-gnaq-picoband-antibody.jpgAnti-GNAQ Antibody Picoband&reg; IHC analysis of GNAQ using anti-GNAQ antibody (PB9707). <br> GNAQ was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GNAQ Antibody (PB9707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9707-5.jpgAnti-GNAQ Antibody Picoband&reg; IF analysis of GNAQ using anti-GNAQ antibody (PB9707). <br> GNAQ was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GNAQ Antibody (PB9707) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9707-6.jpgAnti-GNAQ Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-GNAQ antibody (PB9707).<br>Overlay histogram showing A431 cells stained with PB9707 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNAQ Antibody (PB9707,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grk5-picoband-trade-antibody-pb9708-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9708-grk5-primary-antibodies-wb-testing-1.jpgAnti-GRK5 Antibody Picoband&reg; Western blot analysis of GRK5 using anti-GRK5 antibody (PB9708). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: rat heart tissue lysates,<br> Lane 5: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK5 antigen affinity purified polyclonal antibody (Catalog # PB9708) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRK5 at approximately 68 kDa. The expected band size for GRK5 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9708-2-IHC-anti-grk5-picoband-antibody.jpgAnti-GRK5 Antibody Picoband&reg; IHC analysis of GRK5 using anti-GRK5 antibody (PB9708). <br> GRK5 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GRK5 Antibody (PB9708) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9708-3-IHC-anti-grk5-picoband-antibody.jpgAnti-GRK5 Antibody Picoband&reg; IHC analysis of GRK5 using anti-GRK5 antibody (PB9708). <br> GRK5 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GRK5 Antibody (PB9708) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9708-4-IHC-anti-grk5-picoband-antibody.jpgAnti-GRK5 Antibody Picoband&reg; IHC analysis of GRK5 using anti-GRK5 antibody (PB9708). <br> GRK5 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GRK5 Antibody (PB9708) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9708-grk5-primary-antibodies-wb-testing-5.jpgAnti-GRK5 Antibody Picoband&reg; Western blot analysis of GRK5 using anti-GRK5 antibody (PB9708). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5%(w/v) milk in TBST for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK5 antibody (PB9708) at 1 ug/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with an anti-rabbit IgG(cell signaling,#7074) at 1:1000 for 2 hour at RT. The signal is developed using an Kodak Image Station 4000R Pro;Thermo Scientific West Femto Maximum Sensitivity Substrate(#34096). A specific band was detected for GRK5 at approximately 68 kDa. The expected band size for GRK5 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-grk6-picoband-trade-antibody-pb9709-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9709-grk6-primary-antibodies-wb-testing-1.jpgAnti-GRK6 Antibody Picoband&reg;Western blot analysis of GRK6 using anti-GRK6 antibody (PB9709). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK6 antigen affinity purified polyclonal antibody (Catalog # PB9709) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GRK6 at approximately 66 kDa. The expected band size for GRK6 is at 66 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igfbp-3-picoband-trade-antibody-pb9710-boster.html2026-04-03T05:00:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9710-igfbp3-primary-antibodies-wb-testing-1.jpgAnti-IGFBP3 Antibody Picoband&reg;Western blot analysis of IGFBP3 using anti-IGFBP3 antibody (PB9710). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: mouse kidney tissue lysates,<br> Lane 5: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody (PB9710) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 40 kDa. The expected band size for IGFBP3 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9710-2-IHC-anti-igfbp-3-antibody.jpgAnti-IGFBP3 Antibody Picoband&reg; IHC analysis of IGFBP3 using anti-IGFBP3 antibody (PB9710). <br> IGFBP3 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IGFBP3 Antibody (PB9710) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-igfbp5-picoband-trade-antibody-pb9711-boster.html2026-03-24T05:05:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9711-1-WB-anti-igfbp5-picoband-antibody.jpgAnti-IGFBP5 Antibody Picoband&reg; Western blot analysis of IGFBP5 using anti-IGFBP5 antibody (PB9711). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. <br> Lane 1: U20S Whole Cell Lysate,<br> Lane 2: HELA Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP5 antigen affinity purified polyclonal antibody (Catalog # PB9711) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP5 at approximately 23 kDa. The expected band size for IGFBP5 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9711-13046_2009_article_237_fig2_html.jpgAnti-IGFBP5 Antibody Picoband&reg;Real-time PCR analysis of upregulated or downregulated gene expression in response to HIF-1alpha (A) Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time PCR . In three pairwise comparisons, the upregulation-folds of IGFBP5, IRS4, TNFAIP6, SOCS1, IL-6, VEGF-A mRNA expression were calculated. The mean and standard error are shown (p < 0.05). (B) Aliquots of the same RNA preparations used for microarray hybridization were analyzed by quantitative real-time PCR. In three pairwise comparisons, the downregulation-folds of IGFBP3, ZNF569, SOCS2, SIRPa and XRCC4 mRNA were calculated. The mean and standard error are shown (p < 0.05). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-28-150'>20003295</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9711-13046_2009_article_237_fig3_html.jpgAnti-IGFBP5 Antibody Picoband&reg;Western blot analysis of regulation of protein expression by HIF-1alpha in NCI-H446 cells . According to different treatments, all the cells were divided into four groups: control group (the cells cultured under normoxic conditions of 20% O2), Ad5-HIF-1alpha transfection group, hypoxia group (the cells cultured under normoxic conditions of 1% O2) and Ad5-siHIF-1alpha transfection group (after transfection, the cells were cultured under normoxic conditions of 1% O2). (A) Western blot analysis for IGFBP5 protein expressed by the cells of four groups. (B) Western blot analysis for SOCS1 protein expressed by the cells of four groups. (C) Densitometric analysis of the IGFBP5 and SOCS1 bands compared to the corresponding β-actin bands (*p < 0.05 expression of IGFBP5 or SOCS1 protein in Ad5-HIF-1alpha group vs. control group; ** p < 0.05 expression of IGFBP5 or SOCS1 protein in hypoxia group vs. control group; *** p < 0.05 expression of IGFBP5 or SOCS1 protein in Ad5-siHIF-1alpha group vs. control group). (D) Western blot analysis for IL-6 protein expressed by the cells of four groups. (E) Western blot analysis for STAT3 protein expressed by the cells of four groups. (F) Densitometric analysis of the IL-6 and STAT3 bands compared to the corresponding β-actin bands (*p < 0.05 expression of IL-6 or STAT3 protein in Ad5-HIF-1alpha group vs. Ad5-siHIF-1alpha group group.) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1756-9966-28-150'>20003295</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-involucrin-picoband-trade-antibody-pb9712-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9712-involucrin-primary-antibodies-wb-testing-1.jpgAnti-Involucrin/IVL Antibody Picoband&reg; Western blot analysis of Involucrin/IVL using anti-Involucrin/IVL antibody (PB9712). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hacat whole cell lysates,<br> Lane 2: human RT4 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Involucrin/IVL antigen affinity purified polyclonal antibody (Catalog # PB9712) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Involucrin/IVL at approximately 120 kDa. The expected band size for Involucrin/IVL is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kat2a-gcn5-picoband-trade-antibody-pb9713-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9713-1-WB-anti-kat2a-gcn5-picoband-antibody.jpgAnti-KAT2A/GCN5 Antibody Picoband&reg; Western blot analysis of KAT2A/GCN5 using anti-KAT2A/GCN5 antibody (PB9713). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. <br> Lane 1: A431 Whole Cell Lysate,<br> Lane 2: 22RV1 Whole Cell Lysate,<br> Lane 3: COLO320 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT2A/GCN5 antigen affinity purified polyclonal antibody (Catalog # PB9713) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT2A/GCN5 at approximately 94 kDa. The expected band size for KAT2A/GCN5 is at 94 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kiaa1524-picoband-trade-antibody-pb9714-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9714-1-WB-anti-kiaa1524-cip2a-picoband-antibody.jpgAnti-p90 Autoantigen/KIAA1524 CIP2A Antibody Picoband&reg; Western blot analysis of KIAA1524 using anti-KIAA1524 antibody (PB9714). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Mouse Testis Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIAA1524 antigen affinity purified polyclonal antibody (Catalog # PB9714) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KIAA1524 at approximately 90 kDa. The expected band size for KIAA1524 is at 102 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cytokeratin-19-picoband-trade-antibody-pb9715-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-cytokeratin-19-primary-antibodies-wb-testing-1.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates. <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human SW620 whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: human PANC-1 whole cell lysates, <br> Lane 6: rat lung tissue lysates, <br> Lane 7: rat small intestine tissue lysates, <br> Lane 8: mouse lung tissue lysates, <br> Lane 9: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 19 antigen affinity purified polyclonal antibody (Catalog # PB9715) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 19 at approximately 44 kDa. The expected band size for Cytokeratin 19 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-cytokeratin-19-primary-antibodies-wb-testing-1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1-2: Human keratinocytes isolated from skin.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 19 antigen affinity purified polyclonal antibody (Catalog # PB9715) at 1:4000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with ChemiDoc MP system. A specific band was detected for Cytokeratin 19 at approximately 44 kDa. The expected band size for Cytokeratin 19 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-fphar-16-1552174-g002.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg;Isolation and identification of rat AFCs and NPCs. (A) Morphological map of rat tail IVD using hematoxylin–eosin staining, AF: annulus fibrosus, NP: nucleus pulposus. (B) Annulus fibrosus cells (AFCs) and nucleus pulposus cells (NPCs) cultured after tissue dissociation and collagenase digestion, P1: the first generation, P3: the third generation. (C) Expressions of cytl1 and col1 genes in AF tissue and AFCs were detected by RT-qPCR. (D) Western blotting was used to detect the differences in the expressions of Cytl1 and Col1 in AF tissues and AFCs. (E) Expression levels of Sox9 , krt19 , and col2a1 genes in the NP and NPCs were detected by RT-qPCR. (F) Western blotting was used to detect the differences in the expression levels of Sox9 and KRT19 in the NP and NPCs. Data are presented as the mean ± SD in (D, F) , * p < 0.05, ns: not significant by two-tailed Student’s t-test.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1552174/full'>40213695</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-2_1.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-3_1.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-4_1.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-5.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in paraffin-embedded section of mouse small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogenhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-6.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in paraffin-embedded section of rat small intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-cytokeratin-19-primary-antibodies-ih-testing-7.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-ihc-testing-8.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-ihc-testing-9.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-ihc-testing-10_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-ihc-testing-11_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-ihc-testing-12_2.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-ihc-testing-13_2.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-14_1.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-15_1.jpgAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-16_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-17_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-18_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-19_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-20_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-if-testing-21_1.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; IF analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PB9715). <br> Cytokeratin 19 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-Cytokeratin 19 Antibody (PB9715) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9715-krt19-primary-antibodies-fcm-testing-22.pngAnti-Cytokeratin 19/KRT19 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-Cytokeratin 19 antibody (PB9715).<br>Overlay histogram showing MCF-7 cells stained with PB9715 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cytokeratin 19 Antibody (PB9715,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-limk1-picoband-trade-antibody-pb9716-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9716-1-WB-anti-limk1-lim-kinase-1-picoband-antibody.jpgAnti-LIMK1 Antibody Picoband&reg; Western blot analysis of LIMK1 using anti-LIMK1 antibody (PB9716). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Gaster Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: U87 Whole Cell Lysate at 40ug,<br> Lane 5: SKOV Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIMK1 antigen affinity purified polyclonal antibody (Catalog # PB9716) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIMK1 at approximately 72 kDa. The expected band size for LIMK1 is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lim-kinase-2-picoband-trade-antibody-pb9717-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9717-1-WB-anti-lim-kinase-2-picoband-antibody.jpgAnti-LIM kinase 2/LIMK2 Antibody Picoband&reg; Western blot analysis of LIM kinase 2 using anti-LIM kinase 2 antibody (PB9717). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Mouse Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Liver Tissue Lysate at 50ug,<br> Lane 3: Mouse Thymus Tissue Lysate at 50ug,<br> Lane 4: Mouse Testis Tissue Lysate at 50ug,<br> Lane 5: 293T Whole Cell Lysate at 40ug,<br> Lane 6: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIM kinase 2 antigen affinity purified polyclonal antibody (Catalog # PB9717) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LIM kinase 2 at approximately 72 kDa. The expected band size for LIM kinase 2 is at 72 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lox-picoband-trade-antibody-pb9718-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9718-lox-primary-antibodies-wb-testing-1_1.jpgAnti-LOX Antibody Picoband&reg; Western blot analysis of LOX using anti-LOX antibody (PB9718). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat lung tissue lysates, <br> Lane 3: mouse liver tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOX antigen affinity purified polyclonal antibody (Catalog # PB9718) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOX at approximately 47 kDa. The expected band size for LOX is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-loxl1-picoband-trade-antibody-pb9719-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9719-1-WB-anti-loxl1-picoband-antibody.jpgAnti-LOXL1 Antibody Picoband&reg; Western blot analysis of LOXL1 using anti-LOXL1 antibody (PB9719). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. <br> Lane 1: A549 Whole Cell Lysate,<br> Lane 2: HELA Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOXL1 antigen affinity purified polyclonal antibody (Catalog # PB9719) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOXL1 at approximately 63 kDa. The expected band size for LOXL1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9719-2-IHC-anti-loxl1-picoband-antibody.jpgAnti-LOXL1 Antibody Picoband&reg; IHC analysis of LOXL1 using anti-LOXL1 antibody (PB9719). <br> LOXL1 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LOXL1 Antibody (PB9719) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9719-fonc-14-1406186-g008.jpgAnti-LOXL1 Antibody Picoband&reg;Immunohisctochemical detection of risk gene in GBM tissues. (A, B) LOXL1 immunohistochemistry: the positive expression was mainly in the cytoplasm and a few in the nucleus; (C, F, I) The %Area of risk gene in different group; (D, E) LOXL4 immunohistochemistry: positive localization in cytoplasm; (G, H) GUCA1A immunohistochemistry: positive localization in cytoplasm. Overall survival <15 months group compared to overall survival ≥15 months group ***p<0.001 and ****p<0.0001, N=3. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11403407/'>39286023</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-loxl2-picoband-trade-antibody-pb9720-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9720-loxl2-primary-antibodies-wb-testing-1_1.jpgAnti-LOXL2 Antibody Picoband&reg; Western blot analysis of LOXL2 using anti-LOXL2 antibody (PB9720). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human MDA-MB-453 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LOXL2 antigen affinity purified polyclonal antibody (Catalog # PB9720) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LOXL2 at approximately 105 kDa. The expected band size for LOXL2 is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sapk4-picoband-trade-antibody-pb9721-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-wb-testing-1.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;Western blot analysis of SAPK4 using anti-SAPK4 antibody (PB9721). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human TE-1 whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human Caco-2 whole cell lysates,<br> Lane 5: rat small intestine tissue lysates,<br> Lane 6: mouse small intestine tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAPK4 antigen affinity purified polyclonal antibody (PB9721) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SAPK4 at approximately 42 kDa. The expected band size for SAPK4 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-ihc-testing-1.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;IHC analysis of SAPK4 using anti-SAPK4 antibody (PB9721). <br>SAPK4 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAPK4 Antibody (PB9721) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-ihc-testing-2.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;IHC analysis of SAPK4 using anti-SAPK4 antibody (PB9721). <br>SAPK4 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAPK4 Antibody (PB9721) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-ihc-testing-3.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;IHC analysis of SAPK4 using anti-SAPK4 antibody (PB9721). <br>SAPK4 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAPK4 Antibody (PB9721) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-ihc-testing-4.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;IHC analysis of SAPK4 using anti-SAPK4 antibody (PB9721). <br>SAPK4 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SAPK4 Antibody (PB9721) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-if-testing-1.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;IF analysis of SAPK4 using anti-SAPK4 antibody (PB9721) and anti-Alpha Tubulin antibody (M03989-3). <br>SAPK4 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SAPK4 Antibody (PB9721) and mouse anti-Alpha Tubulin antibody (M03989-3) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-ip-testing-1.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;Immunoprecipitating (IP) SAPK4 in RT4 whole cell lysate.<br> Western blot analysis of SAPK4 using anti-SAPK4 antibody (PB9721); <br> Lane 1: RT4 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-SAPK4 antibody in RT4 whole cell lysate;<br> Lane 3: anti-SAPK4 antibody (2μg) + RT4 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SAPK4 antigen affinity purified polyclonal antibody (PB9721) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for SAPK4 at approximately 42 kDa. The expected band size for SAPK4 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9721-sapk4-primary-antibodies-fcm-testing-1.jpgAnti-SAPK4/MAPK13 Antibody Picoband&reg;Flow Cytometry analysis of CACO-2 cells using anti-SAPK4 antibody (PB9721). <br> Overlay histogram showing CACO-2 cells stained with PB9721 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SAPK4 Antibody (PB9721, 1 μg/1x10⁶ cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mcm8-picoband-trade-antibody-pb9722-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9722-mcm8-primary-antibodies-wb-testing-1.jpgAnti-MCM8 Antibody Picoband&reg; Western blot analysis of MCM8 using anti-MCM8 antibody (PB9722). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM8 antigen affinity purified polyclonal antibody (Catalog # PB9722) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCM8 at approximately 94 kDa. The expected band size for MCM8 is at 94 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mgst1-picoband-trade-antibody-pb9723-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9723-mgst1-primary-antibodies-wb-testing-1_1.jpgAnti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband&reg; Western blot analysis of MGST1 using anti-MGST1 antibody (PB9723). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human RT4 whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human SiHa whole cell lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse testis tissue lysates,<br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGST1 antigen affinity purified polyclonal antibody (PB9723) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MGST1 at approximately 15 kDa. The expected band size for MGST1 is at 10, 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9723-mgst1-primary-antibodies-ihc-testing-4.jpgAnti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband&reg;IHC analysis of MGST1 using anti-MGST1 antibody (PB9723). <br>MGST1 was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MGST1 Antibody (PB9723) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9723-mgst1-primary-antibodies-ihc-testing-2.jpgAnti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband&reg; IHC analysis of MGST1 using anti-MGST1 antibody (PB9723). <br> MGST1 was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MGST1 Antibody (PB9723) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9723-mgst1-primary-antibodies-ihc-testing-3.jpgAnti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband&reg; IHC analysis of MGST1 using anti-MGST1 antibody (PB9723). <br> MGST1 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MGST1 Antibody (PB9723) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9723-mgst1-primary-antibodies-ip-testing-1.jpgAnti-Microsomal Glutathione S-transferase 1/MGST1 Antibody Picoband&reg;Immunoprecipitating (IP) MGST1 in A549 whole cell lysate.<br> Western blot analysis of MGST1 using anti-MGST1 antibody (PB9723); <br> Lane 1: A549 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-MGST1 antibody in A549 whole cell lysate;<br> Lane 3: anti-MGST1 antibody (2μg) + A549 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MGST1 antigen affinity purified polyclonal antibody (PB9723) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for MGST1 at approximately 18 kDa. The expected band size for MGST1 is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mlh1-picoband-trade-antibody-pb9724-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9724-1_2.jpgAnti-MLH1 Antibody Picoband&reg; Western blot analysis of MLH1 using anti-MLH1 antibody (PB9724). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEK293 whole cell lysates&#44; <br> Lane 2: human Hela whole cell lysates&#44; <br> Lane 3: human COLO-320 whole cell lysates&#44; <br> Lane 4: human T-47D whole cell lysates&#44;<br> Lane 5: human A549 whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 antigen affinity purified polyclonal antibody (Catalog # PB9724) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MLH1 at approximately 85KD. The expected band size for MLH1 is at 85KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp1-picoband-trade-antibody-pb9725-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9725-1-WB-anti-mmp-1-antibody.jpgAnti-MMP1 Antibody Picoband&reg; Western blot analysis of MMP1 using anti-MMP1 antibody (PB9725). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: SMMC Whole Cell Lysate,<br> Lane 2: 22RV1 Whole Cell Lysate,<br> Lane 3: MCF-7 Whole Cell Lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (Catalog # PB9725) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP1 at approximately 54KD. The expected band size for MMP1 is at 54KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9725-fphar-16-1682504-g005.jpgAnti-MMP1 Antibody Picoband&reg;Nti-inflammatory activity of PE against LPS-Induced M1 polarization in RAW264.7 macrophages. (A) Assessment of RAW264.7 cell viability after PE treatment; (B) Determination of the half-inhibitory concentration of PE on RAW264.7 cells (>200 μM) (n = 6); (C) Changes in cell morphology of RAW264.7 cells stimulated with LPS and treated with PE at concentrations of 8, 16, and 32 μM; (D) Measurement of fluorescence intensity of CD86 and INOS in RAW264.7 cells via immunofluorescence staining; (E,F) Quantification of fluorescence intensity from images (D) ; (G–K) Analysis of gene expression of INOS, IL-6, TNF-α, TGF-β, and MMP3 in RAW264.7 cells after modeling and PE treatment by RT-PCR; (L) Evaluation of protein expression of TNF-α, IL-6, MMP1, MMP9, and MMP13 in RAW264.7 cells after modeling and PE treatment; (M) Densitometric analysis of the protein expression shown in image (L) . Data are the mean ± SD of 3 independent experiments. # P < 0.05, ## P < 0.01, vs. Ctrl group; * P < 0.05, ** P < 0.01, vs. LPS group; ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1682504/full'>41230092</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9725-mmp1-primary-antibodies-wb-testing-2.jpgAnti-MMP1 Antibody Picoband&reg; Western blot analysis of MMP1 using anti-MMP1 antibody (PB9725). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human Caco-2 whole cell lysates, <br> Lane 4: human HEK293 whole cell lysates, <br> Lane 5: human THP-1 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP1 antigen affinity purified polyclonal antibody (Catalog # PB9725) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP1 at approximately 54KD. The expected band size for MMP1 is at 54KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp-8-picoband-trade-antibody-pb9726-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9726-1-WB-anti-mmp-8-antibody.jpgAnti-MMP8 Antibody Picoband&reg; Western blot analysis of MMP8 using anti-MMP8 antibody (PB9726). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions. <br> Lane 1: K562 Whole Cell Lysate,<br> Lane 2: JURKAT Whole Cell Lysate. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP8 antigen affinity purified polyclonal antibody (Catalog # PB9726) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP8 at approximately 60KD. The expected band size for MMP8 is at 53KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9726-2-IHC-anti-mmp-8-antibody.jpgAnti-MMP8 Antibody Picoband&reg; IHC analysis of MMP8 using anti-MMP8 antibody (PB9726).<br> MMP8 was detected in paraffin-embedded section of Human Appendicitis Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP8 Antibody (PB9726) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/anti-mmp-8-picoband-trade-antibody-pb9727-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9727-mmp8-primary-antibodies-wb-testing-1.jpgAnti-MMP8 Antibody Picoband&reg;Western blot analysis of MMP8 using anti-MMP8 antibody (PB9727). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates,<br> Lane 2: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP8 antigen affinity purified polyclonal antibody (PB9727) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for MMP8 at approximately 55, 70 kDa. The expected band size for MMP8 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9727-2-IHC-anti-mmp-8-antibody.jpgAnti-MMP8 Antibody Picoband&reg; IHC analysis of MMP8 using anti-MMP8 antibody (PB9727).<br> MMP8 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP8 Antibody (PB9727) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9727-3-IHC-anti-mmp-8-antibody.jpgAnti-MMP8 Antibody Picoband&reg; IHC analysis of MMP8 using anti-MMP8 antibody (PB9727).<br> MMP8 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MMP8 Antibody (PB9727) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-omg-picoband-trade-antibody-pb9730-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9730-1-WB-anti-omg-omgp-picoband-antibody.jpgAnti-Oligodendrocyte myelin glycoprotein/OMG Antibody Picoband&reg; Western blot analysis of OMG using anti-OMG antibody (PB9730). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: U87 Whole Cell Lysate at 40ug. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OMG antigen affinity purified polyclonal antibody (Catalog # PB9730) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OMG at approximately 50KD. The expected band size for OMG is at 50KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-opa1-picoband-trade-antibody-pb9731-boster.html2026-03-25T05:22:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9731-opa1-primary-antibodies-wb-testing-1.jpgAnti-OPA1 Antibody Picoband&reg; Western blot analysis of OPA1 using anti-OPA1 antibody (PB9731). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human PANC-1 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: human T47D whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (Catalog # PB9731) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA1 at approximately 80-100KD. The expected band size for OPA1 is at 80-100KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9731-2-IHC-anti-opa1-picoband-antibody.jpgAnti-OPA1 Antibody Picoband&reg; IHC analysis of OPA1 using anti-OPA1 antibody (PB9731).<br> OPA1 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9731-3-IHC-anti-opa1-picoband-antibody.jpgAnti-OPA1 Antibody Picoband&reg; IHC analysis of OPA1 using anti-OPA1 antibody (PB9731).<br> OPA1 was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9731-4-IHC-anti-opa1-picoband-antibody.jpgAnti-OPA1 Antibody Picoband&reg; IHC analysis of OPA1 using anti-OPA1 antibody (PB9731).<br> OPA1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9731-5.pngAnti-OPA1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-OPA1 antibody (PB9731). <br> Overlay histogram showing U20S cells stained with PB9731 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OPA1 Antibody (PB9731&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9731-opa1-primary-antibodies-if-testing-6.jpgAnti-OPA1 Antibody Picoband&reg; IF analysis of OPA1 using anti-OPA1 antibody (PB9731). <br> OPA1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-OPA1 Antibody (PB9731) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9731-opa1-primary-antibodies-wb-testing-7.jpgAnti-OPA1 Antibody Picoband&reg; Western blot analysis of OPA1 using anti-OPA1 antibody (PB9731).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.<br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates,<br> Lane 3: mouse heart tissue lysates,<br> Lane 4: mouse NIH/3T3 whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (Catalog # PB9731) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPA1 at approximately 80-100KD. The expected band size for OPA1 is at 80-100KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-oncostatin-m-picoband-trade-antibody-pb9732-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9732-1-WB-anti-osm-oncostatin-m-antibody.jpgAnti-Oncostatin M/OSM Antibody Picoband&reg; Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (PB9732). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: A549 Whole Cell Lysate,<br> Lane 2: HUT Whole Cell Lysate,<br> Lane 3: JURKAT Whole Cell Lysate,<br> Lane 4: SW620 Whole Cell Lysate,<br> Lane 5: MCF-7 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody (Catalog # PB9732) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 40 kDa. The expected band size for Oncostatin M is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9732-2-IHC-anti-osm-oncostatin-m-antibody.jpgAnti-Oncostatin M/OSM Antibody Picoband&reg; IHC analysis of Oncostatin M using anti-Oncostatin M antibody (PB9732). <br> Oncostatin M was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Oncostatin M Antibody (PB9732) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pak5-picoband-trade-antibody-pb9733-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9733-pak5-primary-antibodies-wb-testing-1.jpgAnti-PAK5/PAK7 Antibody Picoband&reg; Western blot analysis of PAK5 using anti-PAK5 antibody (PB9733). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK5 antigen affinity purified polyclonal antibody (Catalog # PB9733) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAK5 at approximately 81 kDa. The expected band size for PAK5 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9733-pak7-primary-antibodies-ihc-testing-3.jpgAnti-PAK5/PAK7 Antibody Picoband&reg;IHC analysis of PAK5/PAK7 using anti-PAK5/PAK7 antibody (PB9733). <br>PAK5/PAK7 was detected in a paraffin-embedded section of human cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PAK5/PAK7 Antibody (PB9733) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9733-2-IHC-anti-pak5-picoband-antibody.jpgAnti-PAK5/PAK7 Antibody Picoband&reg; IHC analysis of PAK5 using anti-PAK5 antibody (PB9733). <br> PAK5 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PAK5 Antibody (PB9733) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pax2-picoband-trade-antibody-pb9734-boster.html2026-03-24T05:05:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9734-1-WB-anti-pax2-picoband-antibody.jpgAnti-Pax2 Antibody Picoband&reg; Western blot analysis of Pax2 using anti-Pax2 antibody (PB9734). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.<br> Lane 1: Rat Brain Tissue Lysate,<br> Lane 2: Rat Lung Tissue Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pax2 antigen affinity purified polyclonal antibody (Catalog # PB9734) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pax2 at approximately 45 kDa. The expected band size for Pax2 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9734-2-IHC-anti-pax2-picoband-antibody.jpgAnti-Pax2 Antibody Picoband&reg; IHC analysis of Pax2 using anti-Pax2 antibody (PB9734). <br> Pax2 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Pax2 Antibody (PB9734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9734-3-IHC-anti-pax2-picoband-antibody.jpgAnti-Pax2 Antibody Picoband&reg; IHC analysis of Pax2 using anti-Pax2 antibody (PB9734). <br> Pax2 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Pax2 Antibody (PB9734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9734-4-IHC-anti-pax2-picoband-antibody.jpgAnti-Pax2 Antibody Picoband&reg; IHC analysis of Pax2 using anti-Pax2 antibody (PB9734). <br> Pax2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Pax2 Antibody (PB9734) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdpk1-picoband-trade-antibody-pb9735-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9735-1-WB-anti-pdpk1-pdk-1-picoband-antibody.jpgAnti-PDPK1 Antibody Picoband&reg; Western blot analysis of PDPK1 using anti-PDPK1 antibody (PB9735). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Liver Tissue Lysate&#44; <br> Lane 2: Rat Lung Tissue Lysate&#44; <br> Lane 3: Mouse Liver Tissue Lysate&#44; <br> Lane 4: Mouse Lung Tissue Lysate&#44; <br> Lane 5: COLO320 Whole Cell Lysate&#44; <br> Lane 6: MCF-7 Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody (Catalog # PB9735) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9735-fphar-16-1582677-g005.jpgAnti-PDPK1 Antibody Picoband&reg;BECCs regulate PI3K, PDPK1, and mTOR protein levels in HAPH rats. (A–C) Primitive bands of p-PI3K, PI3K, p-PDPK1, PDPK1, p-mTOR, and mTOR by Western blots in lung tissues. (D–F) Quantitative evaluation of p-PI3K, PI3K, p-PDPK1, PDPK1, p-mTOR, and mTOR in lung tissues. n = 5. All data are represented as the mean ± SD. * p < 0.05 vs. control group and # p < 0.05 vs. hypoxia group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9735-fphar-16-1582677-g007.jpgAnti-PDPK1 Antibody Picoband&reg;Flavonoids inhibit the proliferation of PASMCs under hypoxic conditions by inhibiting the PI3K/AKT axis. (A, B) Primitive bands and quantitative densities of p-AKT1 Ser473 and AKT1 with or without Sc79 (20 μM) by Western blots in PASMCs under 3% O 2 . (C, D) Primitive bands and quantitative densities of p-PI3K, PI3K, p-AKT1 Ser473, and AKT1 with or without 740Y-P (10 μM) by Western blots in PASMCs under 3% O 2 . (E, F) Primitive bands and quantitative densities of p-PDPK1, PDPK, p-AKT1 Ser473, and AKT1 with or without MYH1485 (2 μM) in PASMCs by Western blots under 3% O 2 . n = 3. All data are represented as the mean ± SD. * p < 0.05 vs. control group, # p < 0.05 vs. 3% O 2 group, and p < 0.05 vs. 3% O 2 + Fla-50 μg/ml group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1582677/full'>40385484</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9735-2-IHC-anti-pdpk1-pdk-1-picoband-antibody.jpgAnti-PDPK1 Antibody Picoband&reg; IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735). <br> PDPK1 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9735-3-IHC-anti-pdpk1-pdk-1-picoband-antibody.jpgAnti-PDPK1 Antibody Picoband&reg; IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735). <br> PDPK1 was detected in paraffin-embedded section of Rat Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9735-4-IHC-anti-pdpk1-pdk-1-picoband-antibody.jpgAnti-PDPK1 Antibody Picoband&reg; IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735). <br> PDPK1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9735-pdpk1-primary-antibodies-ihc-testing-5.jpgAnti-PDPK1 Antibody Picoband&reg; IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735).<br> PDPK1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9735-pdpk1-primary-antibodies-ihc-testing-6.jpgAnti-PDPK1 Antibody Picoband&reg; IHC analysis of PDPK1 using anti-PDPK1 antibody (PB9735).<br> PDPK1 was detected in frozen section of mouse small intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDPK1 Antibody (PB9735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-por-picoband-trade-antibody-pb9736-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9736-por-primary-antibodies-wb-testing-1.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; Western blot analysis of POR using anti-POR antibody (PB9736). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: rat kidney tissue lysates, <br> Lane 6: rat liver tissue lysates, <br> Lane 7: mouse kidney tissue lysates, <br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POR antigen affinity purified polyclonal antibody (Catalog # PB9736) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POR at approximately 77 kDa. The expected band size for POR is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9736-2-IHC-anti-por-cytochrome-p450-reductase-picoband-antibody.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; IHC analysis of POR using anti-POR antibody (PB9736).<br> POR was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POR Antibody (PB9736) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9736-3-IHC-anti-por-cytochrome-p450-reductase-picoband-antibody.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; IHC analysis of POR using anti-POR antibody (PB9736).<br> POR was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POR Antibody (PB9736) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9736-4-IHC-anti-por-cytochrome-p450-reductase-picoband-antibody.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; IHC analysis of POR using anti-POR antibody (PB9736).<br> POR was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-POR Antibody (PB9736) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9736-por-primary-antibodies-fc-testing-5_1.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-POR antibody (PB9736).<br>Overlay histogram showing SiHa cells stained with PB9736 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POR Antibody (PB9736&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9736-por-primary-antibodies-fc-testing-6.jpgAnti-Cytochrome P450 Reductase/POR Antibody Picoband&reg;6. Flow Cytometry analysis of K562 cells using anti-POR antibody (PB9736).<br>Overlay histogram showing K562 cells stained with PB9736 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POR Antibody (PB9736&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppp1r12a-picoband-trade-antibody-pb9737-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-wb-testing-1.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HELA whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human HEK293 whole cell lysates, <br> Lane 4: monkey COS-7 whole cell lysates, <br> Lane 5: human Raji whole cell lysates, <br> Lane 6: human K562 whole cell lysates, <br> Lane 7: human CACO-2 whole cell lysates, <br> Lane 8: human HEPG2 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (Catalog # PB9737) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 130KD. The expected band size for PPP1R12A is at 130KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9737-2-IHC-anti-ppp1r12a-picoband-antibody.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737).<br> PPP1R12A was detected in paraffin-embedded section of Human Glioma Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-fc-testing-3.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-PPP1R12A antibody (PB9737).<br>Overlay histogram showing Hela cells stained with PB9737 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R12A Antibody (PB9737&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-fc-testing-4.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-PPP1R12A antibody (PB9737).<br>Overlay histogram showing SiHa cells stained with PB9737 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R12A Antibody (PB9737&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-fc-testing-5.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-PPP1R12A antibody (PB9737).<br>Overlay histogram showing U251 cells stained with PB9737 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R12A Antibody (PB9737&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-icc-testing-6.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737).<br> PPP1R12A was detected in immunocytochemical section of SiHa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-icc-testing-7.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737).<br> PPP1R12A was detected in immunocytochemical section of U251 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-icc-testing-8.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737).<br> PPP1R12A was detected in immunocytochemical section of U251 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-icc-testing-9.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737).<br> PPP1R12A was detected in immunocytochemical section of U251 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-icc-testing-10.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; IHC analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737).<br> PPP1R12A was detected in immunocytochemical section of U251 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/ml rabbit anti-PPP1R12A Antibody (PB9737) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using DyLight®488 Conjugated Avidin (BA1128). Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9737-ppp1r12a-primary-antibodies-wb-testing-11.jpgAnti-Myosin Phosphatase/PPP1R12A Antibody Picoband&reg; Western blot analysis of PPP1R12A using anti-PPP1R12A antibody (PB9737). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat lung tissue lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: rat C6 whole cell lysates, <br> Lane 5: mouse brain tissue lysates, <br> Lane 6: mouse lung tissue lysates, <br> Lane 7: mouse liver tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R12A antigen affinity purified polyclonal antibody (Catalog # PB9737) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R12A at approximately 130KD. The expected band size for PPP1R12A is at 130KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ampk-beta-1-picoband-trade-antibody-pb9738-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9738-1-WB-anti-ampk-beta-1-picoband-antibody.jpgAnti-AMPK beta 1/PRKAB1 Antibody Picoband&reg; Western blot analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: JURKAT Whole Cell Lysate,<br> Lane 2: PANC Whole Cell Lysate,<br> Lane 3: K562 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK beta 1 antigen affinity purified polyclonal antibody (Catalog # PB9738) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK beta 1 at approximately 38 kDa. The expected band size for AMPK beta 1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9738-2.jpgAnti-AMPK beta 1/PRKAB1 Antibody Picoband&reg; IF analysis of AMPK beta 1 using anti-AMPK beta 1 antibody (PB9738). <br> AMPK beta 1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AMPK beta 1 Antibody (PB9738) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ampk-beta-2-picoband-trade-antibody-pb9739-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9739-1-WB-anti-ampk-beta-2-picoband-antibody.jpgAnti-AMPK beta 2/PRKAB2 Antibody Picoband&reg; Western blot analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (PB9739). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK beta 2 antigen affinity purified polyclonal antibody (Catalog # PB9739) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK beta 2 at approximately 30 kDa. The expected band size for AMPK beta 2 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9739-2-IHC-anti-ampk-beta-2-picoband-antibody.jpgAnti-AMPK beta 2/PRKAB2 Antibody Picoband&reg; IHC analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (PB9739). <br> AMPK beta 2 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AMPK beta 2 Antibody (PB9739) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9739-3-IHC-anti-ampk-beta-2-picoband-antibody.jpgAnti-AMPK beta 2/PRKAB2 Antibody Picoband&reg; IHC analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (PB9739). <br> AMPK beta 2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AMPK beta 2 Antibody (PB9739) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9739-4-IHC-anti-ampk-beta-2-picoband-antibody.jpgAnti-AMPK beta 2/PRKAB2 Antibody Picoband&reg; IHC analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (PB9739). <br> AMPK beta 2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AMPK beta 2 Antibody (PB9739) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9739-5.pngAnti-AMPK beta 2/PRKAB2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-AMPK beta 2 antibody (PB9739). <br> Overlay histogram showing A431 cells stained with PB9739 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMPK beta 2 Antibody (PB9739&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9739-6.jpgAnti-AMPK beta 2/PRKAB2 Antibody Picoband&reg; IF analysis of AMPK beta 2 using anti-AMPK beta 2 antibody (PB9739). <br> AMPK beta 2 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AMPK beta 2 Antibody (PB9739) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ptp4a2-picoband-trade-antibody-pb9740-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9740-ptp4a2-primary-antibodies-wb-testing-1.jpgAnti-PTP4A2 Antibody Picoband&reg; Western blot analysis of PTP4A2 using anti-PTP4A2 antibody (PB9740). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MOLT-4 whole cell lysates,<br> Lane 2: human T-47D whole cell lysates,<br> Lane 3: human Daudi whole cell lysates,<br> Lane 4: human RT4 whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTP4A2 antigen affinity purified polyclonal antibody (Catalog # PB9740) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTP4A2 at approximately 19 kDa. The expected band size for PTP4A2 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9740-2-IHC-anti-ptp4a2-prl-2-picoband-antibody.jpgAnti-PTP4A2 Antibody Picoband&reg; IHC analysis of PTP4A2 using anti-PTP4A2 antibody (PB9740).<br> PTP4A2 was detected in paraffin-embedded section of Human Prostatic Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PTP4A2 Antibody (PB9740) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9740-ptp4a2-primary-antibodies-fcm-testing-3.jpgAnti-PTP4A2 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-PTP4A2 antibody (PB9740). <br> Overlay histogram showing MCF-7 cells stained with PB9740 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTP4A2 Antibody (PB9740, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9740-ptp4a2-primary-antibodies-fc-testing-4.jpgAnti-PTP4A2 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-PTP4A2 antibody (PB9740).<br>Overlay histogram showing PC-3 cells stained with PB9740 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTP4A2 Antibody (PB9740&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9740-ptp4a2-primary-antibodies-fc-testing-5.jpgAnti-PTP4A2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-PTP4A2 antibody (PB9740).<br>Overlay histogram showing A431 cells stained with PB9740 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTP4A2 Antibody (PB9740&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-c-rel-picoband-trade-antibody-pb9741-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9741-rel-primary-antibodies-wb-testing-1.jpgAnti-c-Rel Antibody Picoband&reg; Western blot analysis of c-Rel using anti-c-Rel antibody (PB9741). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates, <br> Lane 2: rat spleen tissue lysates, <br> Lane 3: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Rel antigen affinity purified polyclonal antibody (Catalog # PB9741) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Rel at approximately 69 kDa. The expected band size for c-Rel is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9741-2-IHC-anti-c-rel-picoband-antibody.jpgAnti-c-Rel Antibody Picoband&reg; IHC analysis of c-Rel using anti-c-Rel antibody (PB9741). <br> c-Rel was detected in a paraffin-embedded section of mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-c-Rel Antibody (PB9741) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9741-12931_2023_2557_fig5_html.pngAnti-c-Rel Antibody Picoband&reg;IL-25 inhibited LPS-induced translocation of c-Rel in STAT3-dependent manner in pulmonary macrophages. ( A ) The level of STAT3 phosphorylation was detected using Western blot. ( B ) Quantitative analysis of STAT3 phosphorylation in primary culture of pulmonary macrophages using ImageJ. Values are expressed in arbitrary units (a.u.). ( C - D ) Western blot was performed to measure the protein levels of c-Rel in the cytoplasm ( C ) and nucleus ( D ) of pulmonary macrophages. ( E - F ) Quantitative analysis of c-Rel expresion in cytoplasm ( E ) and nucleus ( F ) of pulmonary macrophages using ImageJ. ( G ) Representative images of c-Rel immunofluorescence staining in primary culture of macrophage from lung. Scale bar, 50 μm. ( H ) The protein levels of IL-12 A and IL-23 A of pulmonary macrophages was measured using Western blot. ( I - J ) Quantitative analysis of IL-12 A and IL-23 A in pulmonary macrophages using ImageJ. Values are expressed in arbitrary units (a.u.). ( K ) The graphical abstract of this study, which was drawn by Figdraw. The experiment was repeated 3 times independently, and the similar trend was obtained. One way ANOVA was used for statistical analysis (* P < 0.05; ** P < 0.01; *** P < 0.001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12931-023-02557-5'>37898756</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9741-3-IHC-anti-c-rel-picoband-antibody.jpgAnti-c-Rel Antibody Picoband&reg; IHC analysis of c-Rel using anti-c-Rel antibody (PB9741). <br> c-Rel was detected in a paraffin-embedded section of rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-c-Rel Antibody (PB9741) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9741-4-IHC-anti-c-rel-picoband-antibody.jpgAnti-c-Rel Antibody Picoband&reg; IHC analysis of c-Rel using anti-c-Rel antibody (PB9741). <br> c-Rel was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-c-Rel Antibody (PB9741) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s100a8-picoband-trade-antibody-pb9742-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9742-s100a8-primary-antibodies-wb-testing-1_1.jpgAnti-MRP8/S100A8 Antibody Picoband&reg; Western blot analysis of S100A8 using anti-S100A8 antibody (PB9742). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates,<br> Lane 2: rat thymus tissue lysates,<br> Lane 3: mouse spleen tissue lysates,<br> Lane 4: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A8 antigen affinity purified polyclonal antibody (Catalog # PB9742) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A8 at approximately 11 kDa. The expected band size for S100A8 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9742-41467_2020_15186_fig4_html.pngAnti-MRP8/S100A8 Antibody Picoband&reg;S100A8/A9 produced by MDSC induce 18F-FDG-uptake. a The SUV max of on the site of S100A8/A9 heterodimer injection by 18F-FDG-PET/CT. Mice were subcutaneously injected with indicated concentrations of S100A8/A9 heterodimer. 18F-FDG-PET/CT was performed 24 h after the injection, and the SUV max of the injection site was analyzed (PBS, n = 3; others, n = 2). b Representative immunoreactivities of resected paraaortic lymph nodes for S100A8 and S100A9 (ME180-Control-derived tumor- or ME180-GCSF-derived tumor-bearing rats). Scale bars, 50 μm. c Representative image of S100a8 and S100a9 mRNA expression in MDSC of ME180-GCSF-derived tumor-bearing mice as evaluated by RT-PCR. bp, base pairs. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-020-15186-z'>32170086</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9742-41467_2020_15186_fig5_html.pngAnti-MRP8/S100A8 Antibody Picoband&reg;Immunoreactivities of resected lymph nodes for CD33 and S100A8/9 in cervical cancer patients TRL-positive versus TRL-negative patients. a CD33 expression in cervical cancer patients according to white blood cell counts. Photographs; representative CD33-stained lymph node sections from cervical cancer patients. A graph; proportion of patients with CD33-high. b S100A8 expression in cervical cancer patients according to white blood cell counts. Photographs; representative S100A8-stained lymph node sections from cervical cancer patients. A graph; proportion of patients with S100A8-high. ( c ) S100A9 expression in cervical cancer patients according to white blood cell counts. Photographs; representative S100A9-stained lymph node sections from cervical cancer patients. A graph; proportion of patients with S100A9-high. Scale bars, 50 μm. Statistical significance was assessed using two-sided Fisher’s exact test (TRL-positive: WBC ≥ 9000/mL, n = 20; TRL-negative: WBC < 9000/mL, n = 20). Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-020-15186-z'>32170086</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9742-2-IHC-anti-s100a8-calgranulin-a-antibody.jpgAnti-MRP8/S100A8 Antibody Picoband&reg; IHC analysis of S100A8 using anti-S100A8 antibody (PB9742). <br> S100A8 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9742-3-IHC-anti-s100a8-calgranulin-a-antibody.jpgAnti-MRP8/S100A8 Antibody Picoband&reg; IHC analysis of S100A8 using anti-S100A8 antibody (PB9742). <br> S100A8 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9742-s100a8-primary-antibodies-icc-testing-4.jpgAnti-MRP8/S100A8 Antibody Picoband&reg; IF analysis of S100A8 using anti-S100A8 antibody (PB9742). <br> S100A8 was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9742-s100a8-primary-antibodies-fcm-testing-5.pngAnti-MRP8/S100A8 Antibody Picoband&reg; Flow Cytometry analysis of HEPA 1-6 cells using anti-S100A8 antibody (PB9742). <br>Overlay histogram showing HEPA 1-6 cells stained with PB9742 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A8 Antibody (PB9742, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-syndecan-1-picoband-trade-antibody-pb9743-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9743-1-WB-anti-sdc1-syndecan-1-antibody.jpgAnti-Syndecan-1/SDC1 Antibody Picoband&reg; Western blot analysis of Syndecan-1 using anti-Syndecan-1 antibody (PB9743). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HT1080 Whole Cell Lysate,<br> Lane 2: MCF-7 Whole Cell Lysate,<br> Lane 3: 293T Whole Cell Lysate,<br> Lane 4: HEPG2 Whole Cell Lysate,<br> Lane 5: HELA Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syndecan-1 antigen affinity purified polyclonal antibody (Catalog # PB9743) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syndecan-1 at approximately 50 kDa. The expected band size for Syndecan-1 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9743-2.jpgAnti-Syndecan-1/SDC1 Antibody Picoband&reg; IF analysis of Syndecan-1 using anti-Syndecan-1 antibody (PB9743). <br> Syndecan-1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Syndecan-1 Antibody (PB9743) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9743-3.jpgAnti-Syndecan-1/SDC1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-Syndecan-1 antibody (PB9743).<br>Overlay histogram showing A549 cells stained with PB9743 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Syndecan-1 Antibody (PB9743,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-neuroserpin-picoband-trade-antibody-pb9744-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9744-1-WB-anti-neuroserpin-picoband-antibody.jpgAnti-Neuroserpin/SERPINI1 Antibody Picoband&reg; Western blot analysis of Neuroserpin using anti-Neuroserpin antibody (PB9744). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: PANC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neuroserpin antigen affinity purified polyclonal antibody (Catalog # PB9744) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neuroserpin at approximately 46 kDa. The expected band size for Neuroserpin is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc10a1-picoband-trade-antibody-pb9745-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9745-slc10a1-primary-antibodies-fcm-testing-6_1.jpgAnti-SLC10A1 Antibody Picoband&reg; Flow Cytometry analysis of RAW264.7 cells using anti-SLC10A1 antibody (PB9745).<br> Overlay histogram showing RAW264.7 cells stained with PB9745 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC10A1 Antibody (PB9745,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9745-slc10a1-primary-antibodies-wb-testing-1.jpgAnti-SLC10A1 Antibody Picoband&reg; Western blot analysis of SLC10A1 using anti-SLC10A1 antibody (PB9745). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (Catalog # PB9745) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50 kDa. The expected band size for SLC10A1 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9745-slc10a1-primary-antibodies-ihc-testing-2.jpgAnti-SLC10A1 Antibody Picoband&reg; IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PB9745). <br> SLC10A1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SLC10A1 Antibody (PB9745) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9745-slc10a1-primary-antibodies-ihc-testing-3.jpgAnti-SLC10A1 Antibody Picoband&reg; IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PB9745). <br> SLC10A1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SLC10A1 Antibody (PB9745) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9745-slc10a1-primary-antibodies-ihc-testing-4.jpgAnti-SLC10A1 Antibody Picoband&reg; IHC analysis of SLC10A1 using anti-SLC10A1 antibody (PB9745).<br> SLC10A1 was detected in frozen section of mouse liver tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC10A1 Antibody (PB9745) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9745-slc10a1-primary-antibodies-if-testing-5.jpgAnti-SLC10A1 Antibody Picoband&reg; IF analysis of SLC10A1 using anti- SLC10A1 antibody (PB9745).<br> SLC10A1 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti- SLC10A1 Antibody (PB9745) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1037) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smc3-picoband-trade-antibody-pb9746-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9746-1-WB-anti-smc3-picoband-antibody.jpgAnti-SMC3 Antibody Picoband&reg; Western blot analysis of SMC3 using anti-SMC3 antibody (PB9746). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Liver Tissue Lysate at 50ug,<br> Lane 3: Rat Testis Tissue Lysate at 50ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug,<br> Lane 5: A549 Whole Cell Lysate at 40ug,<br> Lane 6: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 7: NIH3T3 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC3 antigen affinity purified polyclonal antibody (Catalog # PB9746) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC3 at approximately 140 kDa. The expected band size for SMC3 is at 140 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9746-2-IHC-anti-smc3-picoband-antibody.jpgAnti-SMC3 Antibody Picoband&reg; IHC analysis of SMC3 using anti-SMC3 antibody (PB9746). <br> SMC3 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SMC3 Antibody (PB9746) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9746-3-IHC-anti-smc3-picoband-antibody.jpgAnti-SMC3 Antibody Picoband&reg; IHC analysis of SMC3 using anti-SMC3 antibody (PB9746). <br> SMC3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SMC3 Antibody (PB9746) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9746-4-IHC-anti-smc3-picoband-antibody.jpgAnti-SMC3 Antibody Picoband&reg; IHC analysis of SMC3 using anti-SMC3 antibody (PB9746). <br> SMC3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SMC3 Antibody (PB9746) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trex1-picoband-trade-antibody-pb9748-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9748-1-WB-anti-trex1-picoband-antibody.jpgAnti-TREX1 Antibody Picoband&reg; Western blot analysis of TREX1 using anti-TREX1 antibody (PB9748). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: SMMC Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREX1 antigen affinity purified polyclonal antibody (Catalog # PB9748) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREX1 at approximately 33 kDa. The expected band size for TREX1 is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prealbumin-picoband-trade-antibody-pb9749-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9749-prealbumin-primary-antibodies-wb-testing-1.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; Western blot analysis of Prealbumin using anti-Prealbumin antibody (PB9749). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human placenta tissue lysate,<br> Lane 2: human HCCP tissue lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prealbumin antigen affinity purified polyclonal antibody (Catalog # PB9749) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prealbumin at approximately 14 kDa. The expected band size for Prealbumin is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9749-2-IHC-anti-prealbumin-picoband-antibody.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; IHC analysis of Prealbumin using anti-Prealbumin antibody (PB9749). <br> Prealbumin was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Prealbumin Antibody (PB9749) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9749-ttr-primary-antibodies-elisa-testing-3.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; Sandwich ELISA - Recombinant human IL15RA protein standard curve.<br> Use in combination with reagents from Human IL15RA ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1663). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prealbumin-picoband-trade-antibody-pb9750-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9750-prealbumin-primary-antibodies-wb-testing-1.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; Western blot analysis of Prealbumin using anti-Prealbumin antibody (PB9750). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysate,<br> Lane 2: rat kidney tissue lysate,<br> Lane 3: mouse liver tissue lysate,<br> Lane 4: mouse kidney tissue lysate,<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prealbumin antigen affinity purified polyclonal antibody (Catalog # PB9750) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prealbumin at approximately 16KD. The expected band size for Prealbumin is at 16KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9750-2-IHC-anti-prealbumin-picoband-antibody.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; IHC analysis of Prealbumin using anti-Prealbumin antibody (PB9750). <br> Prealbumin was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Prealbumin Antibody (PB9750) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9750-3-IHC-anti-prealbumin-picoband-antibody.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; IHC analysis of Prealbumin using anti-Prealbumin antibody (PB9750). <br> Prealbumin was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Prealbumin Antibody (PB9750) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9750-prealbumin-primary-antibodies-if-testing-4.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; IF analysis of Prealbumin using anti-Prealbumin antibody (PB9750). <br> Prealbumin was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Prealbumin Antibody (PB9750) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9750-prealbumin-primary-antibodies-if-testing-5.jpgAnti-Prealbumin/TTR Antibody Picoband&reg; IF analysis of Prealbumin using anti-Prealbumin antibody (PB9750). <br> Prealbumin was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Prealbumin Antibody (PB9750) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erv31-antibody-rp1091-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1091-erv31-primary-antibodies-wb-testing-1.jpgAnti-ERV31/ERV3-1 Antibody Picoband&reg;Western blot analysis of ERV31/ERV3-1 using anti-ERV31/ERV3-1 antibody (RP1091). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human U251 whole cell lysates,<br> Lane 5: human MCF-7 whole cell lysates,<br> Lane 6: human RT4 whole cell lysates,<br> Lane 7: human Jurkat whole cell lysates,<br> Lane 8: human SiHa whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERV31/ERV3-1 antigen affinity purified polyclonal antibody (RP1091) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ERV31/ERV3-1 at approximately 68 kDa. The expected band size for ERV31/ERV3-1 is at 68 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thrombin-receptor-antibody-rp1092-boster.html2026-03-25T05:22:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1092-2-IHC-anti-thrombin-receptor-antibody.jpgAnti-Thrombin Receptor/F2R Antibody Picoband&reg;Anti-Thrombin Receptor antibody&#44; RP1092&#44; IHC(P)<br>IHC(P): Human Placenta Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1092-1-WB-anti-thrombin-receptor-antibody.jpgAnti-Thrombin Receptor/F2R Antibody Picoband&reg;Anti-Thrombin Receptor antibody&#44; RP1092&#44; Western blotting<br>All lanes: Anti Thrombin Receptor (RP1092) at 0.5ug/ml<br> Lane 1: MCF-7 Whole Cell Lysate at 40ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: 22RV1 Whole Cell Lysate at 40ug<br> Lane 4: SW620 Whole Cell Lysate at 40ug<br> Predicted bind size: 47KD<br> Observed bind size: 47KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tim-1-antibody-rp1093-boster.html2026-03-24T05:05:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1093-1-WB-anti-kim1-antibody.jpgAnti-TIM 1/HAVCR1 Antibody Picoband&reg;Anti-TIM1 antibody&#44; RP1093&#44; Western blotting<br>All lanes: Anti TIM1 (RP1093) at 0.5ug/ml<br> Lane 1: HELA Whole Cell Lysate at 40ug<br> Lane 2: PANC Whole Cell Lysate at 40ug<br> Lane 3: HEPG2 Whole Cell Lysate at 40ug<br> Lane 4: A549 Whole Cell Lysate at 40ug<br> Predicted bind size: 39KD<br> Observed bind size: 49KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hsd11b2-antibody-rp1094-boster.html2026-03-30T05:00:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1094-hsd11b2-primary-antibodies-wb-testing-1.jpgAnti-HSD11B2 Antibody Picoband&reg; Western blot analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates,<br> Lane 2: mouse kidney tissue lysates,<br> Lane 3: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD11B2 antigen affinity purified polyclonal antibody (Catalog # RP1094) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD11B2 at approximately 43 kDa. The expected band size for HSD11B2 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1094-2-IHC-anti-hsd11b2-11beta-hsd2-antibody.jpgAnti-HSD11B2 Antibody Picoband&reg; IHC analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> HSD11B2 was detected in a paraffin-embedded section of Mouse Pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD11B2 Antibody (RP1094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1094-41598_2025_20141_fig5_html.pngAnti-HSD11B2 Antibody Picoband&reg;Integration analysis of transcriptomic and metabolomics. ( A ) Bubble map of KEGG enrichment pathway of DEGs and DCMs . ( B ) The levels of cortisol and aldosterone in blood after exposure with BPA measured by ELISA. ( C ) The protein levels of MAPK and ERK in kidney after exposure with BPA. ( D ) The protein levels of 11β-HSD2 in kidney after exposure with BPA. ( E ) The diagram of regulatory mechanisms about the steroid hormone synthesis in the mouse kidney after BPA exposure. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-20141-3'>41107381</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1094-41598_2025_20141_fig6_html.pngAnti-HSD11B2 Antibody Picoband&reg;Cellular validation of MAPK-regulated steroid hormone synthesis induced by BPA exposure. ( A ) Molecular docking model diagram of BPA and MAPK or ERK proteins. ( B ) Protein expression of 11β-HSD2 in TCMK-1 cells after BPA exposure and MAPK inhibitor (Adezmapimod, SB203580) treatment. ( C ) Protein expression of 11β-HSD2 in HK-2 cells after BPA exposure and MAPK inhibitor (Adezmapimod, SB203580) treatment. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-20141-3'>41107381</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1094-3-IHC-anti-hsd11b2-11beta-hsd2-antibody.jpgAnti-HSD11B2 Antibody Picoband&reg; IHC analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> HSD11B2 was detected in a paraffin-embedded section of Rat Pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD11B2 Antibody (RP1094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1094-4-IHC-anti-hsd11b2-11beta-hsd2-antibody.jpgAnti-HSD11B2 Antibody Picoband&reg; IHC analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> HSD11B2 was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HSD11B2 Antibody (RP1094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1094-hsd11b2-primary-antibodies-ihc-f-testing-5.jpgAnti-HSD11B2 Antibody Picoband&reg; IHC analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> HSD11B2 was detected in a frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-HSD11B2 Antibody (RP1094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1094-hsd11b2-primary-antibodies-ihc-f-testing-6.jpgAnti-HSD11B2 Antibody Picoband&reg; IHC analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> HSD11B2 was detected in a frozen section of mouse kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (RP1094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1094-hsd11b2-primary-antibodies-ihc-f-testing-7.jpgAnti-HSD11B2 Antibody Picoband&reg; IHC analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> HSD11B2 was detected in a frozen section of rat kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HSD11B2 Antibody (RP1094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1094-hsd11b2-primary-antibodies-if-testing-8.jpgAnti-HSD11B2 Antibody Picoband&reg; IF analysis of HSD11B2 using anti-HSD11B2 antibody (RP1094). <br> HSD11B2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HSD11B2 Antibody (RP1094) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pu-1-spi1-antibody-rp1097-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1097-1-IHC-anti-pu-1-spi1-antibody.jpgAnti-PU.1/Spi1 AntibodyAnti-PU.1/Spi1 antibody&#44; RP1097&#44; IHC(P)<br>IHC(P): Mouse Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1097-2-IHC-anti-pu-1-spi1-antibody.jpgAnti-PU.1/Spi1 AntibodyAnti-PU.1/Spi1 antibody&#44; RP1097&#44; IHC(P)<br>IHC(P): Rat Spleen Tissuehttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1097-3-IHC-anti-pu-1-spi1-antibody.jpgAnti-PU.1/Spi1 AntibodyAnti-PU.1/Spi1 antibody&#44; RP1097&#44; IHC(P)<br>IHC(P): Human Tonsil Tissue https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-actin-picoband-trade-antibody-pb9751-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9751-actin-primary-antibodies-wb-testing-1.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; Western blot analysis of Actin using anti-Actin antibody (PB9751). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: rat kidney tissue lysates,<br> Lane 4: mouse heart tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse spleen tissue lysates,<br> Lane 7: mouse kidney tissue lysates,<br> Lane 8: human Hela whole cell lysates,<br> Lane 9: human HepG2 whole cell lysates,<br> Lane 10: human 293T whole cell lysates,<br> Lane 11: human A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Actin antigen affinity purified polyclonal antibody (Catalog # PB9751) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Actin at approximately 42 kDa. The expected band size for Actin is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9751-actin-primary-antibodies-ihc-testing-2.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (PB9751). <br> Actin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (PB9751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9751-actin-primary-antibodies-ihc-testing-3.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (PB9751). <br> Actin was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (PB9751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9751-actin-primary-antibodies-ihc-testing-4.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (PB9751). <br> Actin was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (PB9751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9751-actin-primary-antibodies-ihc-testing-5.jpgAnti-Actin/ACTA1 Antibody Picoband&reg; IHC analysis of Actin using anti-Actin antibody (PB9751). <br> Actin was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Actin Antibody (PB9751) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adra1a-picoband-trade-antibody-pb9752-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9752-adra1a-primary-antibodies-wb-testing-1.jpgAnti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband&reg;Western blot analysis of ADRA1A using anti-ADRA1A antibody (PB9752). <br> Electrophoresis was performed on a 10 SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat heart tissue lysates,<br> Lane 3: mouse heart tissue lysates,<br> Lane 4: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA1A antigen affinity purified polyclonal antibody (Catalog # PB9752) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADRA1A at approximately 51 kDa. The expected band size for ADRA1A is at 51 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9752-2.jpgAnti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband&reg; IHC analysis of ADRA1A using anti-ADRA1A antibody (PB9752). <br> ADRA1A was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADRA1A Antibody (PB9752) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9752-3.jpgAnti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband&reg; IHC analysis of ADRA1A using anti-ADRA1A antibody (PB9752). <br> ADRA1A was detected in paraffin-embedded section of rat brain tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADRA1A Antibody (PB9752) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9752-4.jpgAnti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband&reg; IHC analysis of ADRA1A using anti-ADRA1A antibody (PB9752). <br> ADRA1A was detected in paraffin-embedded section of rat brain tissue tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADRA1A Antibody (PB9752) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9752-5.pngAnti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ADRA1A antibody (PB9752). <br> Overlay histogram showing A431 cells stained with PB9752 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADRA1A Antibody (PB9752&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arc-picoband-trade-antibody-pb9753-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9753-arc-primary-antibodies-wb-testing-1.jpgAnti-Arc Antibody Picoband&reg; Western blot analysis of Arc using anti-Arc antibody (PB9753). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U20S whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human SH-SY5Y whole cell lysates,<br> Lane 4: human SiHa whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arc antigen affinity purified polyclonal antibody (Catalog # PB9753) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arc at approximately 45 kDa. The expected band size for Arc is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bag3-picoband-trade-antibody-pb9754-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9754-Bag3-primary-antibodies-WB-testing-1.jpgAnti-Bag3 Antibody Picoband&reg; Western blot analysis of Bag3 using anti-Bag3 antibody (PB9754). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: human U251 whole cell lysates,<br> Lane 6: human U87 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag3 antigen affinity purified polyclonal antibody (Catalog # PB9754) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for Bag3 at approximately 80 kDa. The expected band size for Bag3 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9754-2-IHC-anti-bag3-picoband-antibody.jpgAnti-Bag3 Antibody Picoband&reg; IHC analysis of Bag3 using anti-Bag3 antibody (PB9754). <br> Bag3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Bag3 Antibody (PB9754) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bik-picoband-trade-antibody-pb9755-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9755-1-WB-anti-bik-picoband-antibody.jpgAnti-Bik Antibody Picoband&reg; Western blot analysis of Bik using anti-Bik antibody (PB9755). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Spleen Tissue Lysate&#44;<br> Lane 2: Rat Gaster Tissue Lysate&#44;<br> Lane 3: Rat Intestine Tissue Lysate&#44;<br> Lane 4: MCF-7 Whole Cell Lysate&#44;<br> Lane 5: A549 Whole Cell Lysate&#44;<br> Lane 6: SKOV Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bik antigen affinity purified polyclonal antibody (Catalog # PB9755) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bik at approximately 22KD. The expected band size for Bik is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9755-2-IHC-anti-bik-picoband-antibody.jpgAnti-Bik Antibody Picoband&reg; IHC analysis of Bik using anti-Bik antibody (PB9755). <br> Bik was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bik Antibody (PB9755) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9755-3-IHC-anti-bik-picoband-antibody.jpgAnti-Bik Antibody Picoband&reg; IHC analysis of Bik using anti-Bik antibody (PB9755). <br> Bik was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bik Antibody (PB9755) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9755-4-IHC-anti-bik-picoband-antibody.jpgAnti-Bik Antibody Picoband&reg; IHC analysis of Bik using anti-Bik antibody (PB9755). <br> Bik was detected in paraffin-embedded section of Human Thyroid Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bik Antibody (PB9755) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9755-bik-primary-antibodies-ihc-testing-4.pngAnti-Bik Antibody Picoband&reg;IHC analysis of Bik using anti-Bik antibody (PB9755). <br> Bik was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit antiBik Antibody (PB9755) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9755-bik-primary-antibodies-fc-testing-5.jpgAnti-Bik Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-BIK antibody (PB9755). <br>Overlay histogram showing MCF-7 cells stained with PB9755 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BIK Antibody (PB9755&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9755-bik-primary-antibodies-fc-testing-6.jpgAnti-Bik Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-BIK antibody (PB9755). <br>Overlay histogram showing THP-1 cells stained with PB9755 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BIK Antibody (PB9755&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9755-bik-primary-antibodies-fc-testing-7.jpgAnti-Bik Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-BIK antibody (PB9755). <br>Overlay histogram showing A431 cells stained with PB9755 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BIK Antibody (PB9755&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc25c-picoband-trade-antibody-pb9756-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9756-cdc25c-primary-antibodies-wb-testing-1.jpgAnti-Cdc25C Antibody Picoband&reg;Western blot analysis of Cdc25C using anti-Cdc25C antibody (PB9756). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates.<br> Lane 2: human Hela whole cell lysates.<br> Lane 3: human HepG2 whole cell lysates.<br> Lane 4: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25C antigen affinity purified polyclonal antibody (PB9756) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Cdc25C at approximately 53 kDa. The expected band size for Cdc25C is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9756-cdc25c-primary-antibodies-if-testing-1.jpgAnti-Cdc25C Antibody Picoband&reg;IF analysis of Cdc25C using anti-Cdc25C antibody (PB9756) and anti-Tubulin Alpha antibody (M03989-3).<br> Cdc25C was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cdc25C Antibody (PB9756) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9756-cdc25c-primary-antibodies-fcm-testing-1.jpgAnti-Cdc25C Antibody Picoband&reg;Flow Cytometry analysis of K562 cells using anti-Cdc25C antibody (PB9756). <br> Overlay histogram showing K562 cells stained with PB9756 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cdc25C Antibody (PB9756, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddb2-picoband-trade-antibody-pb9757-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9757-1-WB-anti-ddb2-xpe-picoband-antibody.jpgAnti-DDB2 Antibody Picoband&reg; Western blot analysis of DDB2 using anti-DDB2 antibody (PB9757). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: A431 Whole Cell Lysate,<br> Lane 2: SW620 Whole Cell Lysate,<br> Lane 3: HELA Whole Cell Lysate,<br> Lane 4: JURKAT Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDB2 antigen affinity purified polyclonal antibody (Catalog # PB9757) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDB2 at approximately 48 kDa. The expected band size for DDB2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9757-2.jpgAnti-DDB2 Antibody Picoband&reg; IF analysis of DDB2 using anti-DDB2 antibody (PB9757). <br> DDB2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDB2 Antibody (PB9757) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9757-3.jpgAnti-DDB2 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-DDB2 antibody (PB9757).<br>Overlay histogram showing 293T cells stained with PB9757 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDB2 Antibody (PB9757,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eif4a2-picoband-trade-antibody-pb9758-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9758-eif4a2-primary-antibodies-wb-testing-1.jpgAnti-eIF4A2 Antibody Picoband&reg; Western blot analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human U251 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat lung tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-eIF4A2 antigen affinity purified polyclonal antibody (Catalog # PB9758) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for eIF4A2 at approximately 46 kDa. The expected band size for eIF4A2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9758-2-IHC-anti-eif4a2-eif4aii-picoband-antibody.jpgAnti-eIF4A2 Antibody Picoband&reg; IHC analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). <br> eIF4A2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-eIF4A2 Antibody (PB9758) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9758-3-IHC-anti-eif4a2-eif4aii-picoband-antibody.jpgAnti-eIF4A2 Antibody Picoband&reg; IHC analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). <br> eIF4A2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-eIF4A2 Antibody (PB9758) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9758-4-IHC-anti-eif4a2-eif4aii-picoband-antibody.jpgAnti-eIF4A2 Antibody Picoband&reg; IHC analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). <br> eIF4A2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-eIF4A2 Antibody (PB9758) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9758-6.jpgAnti-eIF4A2 Antibody Picoband&reg; IF analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758).<br> eIF4A2 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-eIF4A2 Antibody (PB9758) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9758-5.pngAnti-eIF4A2 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-eIF4A2 antibody (PB9758). <br> Overlay histogram showing SiHa cells stained with PB9758 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-eIF4A2 Antibody (PB9758&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9758-eif4a2-primary-antibodies-wb-testing-7.jpgAnti-eIF4A2 Antibody Picoband&reg; Western blot analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human U251 whole cell lysates, <br> Lane 3: human SiHa whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat lung tissue lysates,<br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-eIF4A2 antigen affinity purified polyclonal antibody (PB9758) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for eIF4A2 at approximately 46 kDa. The expected band size for eIF4A2 is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9758-eif4a2-primary-antibodies-ip-testing-1.jpgAnti-eIF4A2 Antibody Picoband&reg;Immunoprecipitating (IP) eIF4A2 in K562 whole cell lysate.<br> Western blot analysis of eIF4A2 using anti-eIF4A2 antibody (PB9758); <br> Lane 1: K562 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-eIF4A2 antibody in K562 whole cell lysate;<br> Lane 3: anti-eIF4A2 antibody (2μg) + K562 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-eIF4A2 antigen affinity purified polyclonal antibody (PB9758) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BM2007). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for eIF4A2 at approximately 46 kDa. The expected band size for eIF4A2 is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fmo2-picoband-trade-antibody-pb9760-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9760-fmo2-primary-antibodies-wb-testing-1.jpgAnti-FMO2 Antibody Picoband&reg; Western blot analysis of FMO2 using anti-FMO2 antibody (PB9760). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates,<br> Lane 2: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO2 antigen affinity purified polyclonal antibody (PB9760) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FMO2 at approximately 61 kDa. The expected band size for FMO2 is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il2rb-picoband-trade-antibody-pb9761-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9761-1-WB-anti-il2rb-il-2rbeta-picoband-antibody.jpgAnti-IL2 Receptor beta/IL2RB Antibody Picoband&reg; Western blot analysis of IL2RB using anti-IL2RB antibody (PB9761). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.<br> Lane 1: Mouse Lung Tissue Lysate,<br> Lane 2: Mouse Spleen Tissue Lysate,<br> Lane 3: Mouse Testis Tissue Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RB antigen affinity purified polyclonal antibody (Catalog # PB9761) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL2RB at approximately 75 kDa. The expected band size for IL2RB is at 75 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-keap1-picoband-trade-antibody-pb9762-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9762-keap1-primary-antibodies-wb-testing-1.jpgAnti-Keap1 Antibody Picoband&reg; Western blot analysis of Keap1 using anti-Keap1 antibody (PB9762). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human PC-3 whole cell lysates, <br> Lane 6: human Caco-2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Keap1 antigen affinity purified polyclonal antibody (Catalog # PB9762) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Keap1 at approximately 66 kDa. The expected band size for Keap1 is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek3-picoband-trade-antibody-pb9763-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-12885_2013_article_4097_fig1_html.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg;Immunohistochemistry (IHC) staining determined MAP2K3 expression in human HCC tumor and matched adjacent tissues. A-D : Representative images of MAP2K3 protein expression determined by IHC staining. A : An image represented a negative (-) expression of MAP2K3 expression; B : An image represented a low level (+) expression of MAP2K3, which showed a weak immunoreactive staining in cytoplasm; C : An image represented a negative (++) expression of MAP2K3 expression; D : An image represented a high level (+++) expression of MAP2K3, which exhibited a strong IHC staining in cytoplasm and perinuclear localization (arrowhead). E : Semi-quantitative analysis of MAP2K3 protein expression using integrated absorbance (IA) in human HCC tissues. Value was expressed as the average values from each individual sample of HCC tumor tissues or its matched adjacent tissue. The total average value of IA in the HCC tumor tissues was significantly greater as compared with the matched adjacent tissues ( p <0.05, n = 14). Data was expressed as mean ± SD for 14 sets of samples. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1471-2407-13-469'>24112539</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-12885_2013_article_4097_fig2_html.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg;Validation of MAP2K3 mRNA as a target of miR-21. (A) : Sequence of potential binding site of miR-21 in the 3’UTR of MAP2K3 mRNA (top panel), mutations were introduced into the binding site for generation of mutated MAP2K3 3’TUR (bottom panel). ( B and C ): Validation of miR-21 target using MAP2K3 3’UTR luciferase reporter. Cells co-transfected with pMIR-Report/MAP2K3 3’UTR (WT) or pMIR-Report/Mut-MAP2K3 3’UTR (Mut) and pAd/pri-miR-21 (B) , pAd/miR-21/inhibitor (C) , and pAd/con plasmids showed a decreased luciferase activity in pAd/pri-miR-21 cells (B) . Luciferase activity after site directed mutagenesis of the 3’UTR of MAP2K3 mRNA in the miR-21 seed sequence (pMIR-Report/Mut-MAP2K3) was significantly higher with respect to the pMIR-Report/MAP2K3 vector transfected cells ( B and C ). Results represented the mean ± SD from three independent triplicated experiments (N=9). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1471-2407-13-469'>24112539</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-12885_2013_article_4097_fig4_html.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg;miR-21 targets MAP2K3 mRNA. The HepG2 cells were infected with Ad/pri-miR-21, Ad/miR-21/inhibitor or Ad/con adenoviral vector. The expression of MAP2K3 was detected by immunoblotting analysis against anti-MAP2K3 antibody. Compared with Ad/con group, *: p <0.05. Data in A represented the mean ± SD from three independent triplicated experiments (N=9). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/1471-2407-13-469'>24112539</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-mek3-primary-antibodies-wb-testing-1.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg; Western blot analysis of MEK3 using anti-MEK3 antibody (PB9763). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysates,<br> Lane 2: rat kidney tissue lysates,<br> Lane 3: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PB9763) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 40 kDa. The expected band size for MEK3 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-mek3-primary-antibodies-fcm-testing-5.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-MEK3 antibody (PB9763). <br>Overlay histogram showing CACO-2 cells stained with PB9763 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK3 Antibody (PB9763, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-mek3-primary-antibodies-ihc-testing-2.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg; IHC analysis of MEK3 using anti-MEK3 antibody (PB9763). <br> MEK3 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MEK3 Antibody (PB9763) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-mek3-primary-antibodies-ihc-testing-3.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg; IHC analysis of MEK3 using anti-MEK3 antibody (PB9763). <br> MEK3 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MEK3 Antibody (PB9763) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9763-mek3-primary-antibodies-if-testing-4.jpgAnti-MEK3/MAP2K3 Antibody Picoband&reg; IF analysis of MEK3 using anti-MEK3 antibody (PB9763). <br> MEK3 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4 μg/mL rabbit anti-MEK3 Antibody (PB9763) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mek7-picoband-trade-antibody-pb9764-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9764-mek7-primary-antibodies-fcm-testing-4.pngAnti-MEK7/MAP2K7 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-MEK7/MAP2K7 antibody (PB9764). <br>Overlay histogram showing U87 cells stained with PB9764 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK7/MAP2K7 Antibody (PB9764, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9764-mek7-primary-antibodies-wb-testing-1.jpgAnti-MEK7/MAP2K7 Antibody Picoband&reg; Western blot analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody (PB9764). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: monkey COS-7 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human Raji whole cell lysates, <br> Lane 5: rat testis tissue lysates, <br> Lane 7: mouse testis tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK7/MAP2K7 antigen affinity purified polyclonal antibody (Catalog # PB9764) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK7/MAP2K7 at approximately 47 kDa. The expected band size for MEK7/MAP2K7 is at 47 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9764-mek7-primary-antibodies-ihc-testing-2.jpgAnti-MEK7/MAP2K7 Antibody Picoband&reg; IHC analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody (PB9764). <br> MEK7/MAP2K7 was detected in a paraffin-embedded section of human testicular cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MEK7/MAP2K7 Antibody (PB9764) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9764-mek7-primary-antibodies-if-testing-3.jpgAnti-MEK7/MAP2K7 Antibody Picoband&reg; IF analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody (PB9764). <br> MEK7/MAP2K7 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MEK7/MAP2K7 Antibody (PB9764) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nr3c2-picoband-trade-antibody-pb9765-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9765-nr3c2-primary-antibodies-if-testing-2.jpgAnti-Mineralocorticoid Receptor/NR3C2 Antibody Picoband&reg; IF analysis of NR3C2 using anti-NR3C2 antibody (PB9765) and anti-Tubulin Alpha antibody (M03989-3).<br> NR3C2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-NR3C2 Antibody (PB9765) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9765-nr3c2-primary-antibodies-wb-testing-1.jpgAnti-Mineralocorticoid Receptor/NR3C2 Antibody Picoband&reg; Western blot analysis of NR3C2 using anti-NR3C2 antibody (PB9765). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human LNCAP whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat NRK whole cell lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C2 antigen affinity purified polyclonal antibody (Catalog # PB9765) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR3C2 at approximately 110 kDa. The expected band size for NR3C2 is at 107 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nur77-picoband-trade-antibody-pb9766-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9766-1-WB-anti-nur77-picoband-antibody.jpgAnti-NUR77/NR4A1 Antibody Picoband&reg; Western blot analysis of NUR77 using anti-NUR77 antibody (PB9766). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: U87 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUR77 antigen affinity purified polyclonal antibody (Catalog # PB9766) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUR77 at approximately 67 kDa. The expected band size for NUR77 is at 67 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ogt-picoband-trade-antibody-pb9767-boster.html2026-03-24T05:05:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9767-ogt-primary-antibodies-wb-testing-1.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; Western blot analysis of OGT using anti-OGT antibody (PB9767). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human Caco-2 whole cell lysates,<br> Lane 6: human K562 whole cell lysates,<br> Lane 7: rat heart tissue lysates,<br> Lane 8: mouse heart tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGT antigen affinity purified polyclonal antibody (Catalog # PB9767) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGT at approximately 117KD. The expected band size for OGT is at 117KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9767-2-IHC-anti-ogt-picoband-antibody.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; IHC analysis of OGT using anti-OGT antibody (PB9767). <br> OGT was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-OGT Antibody (PB9767) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9767-3-IHC-anti-ogt-picoband-antibody.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; IHC analysis of OGT using anti-OGT antibody (PB9767). <br> OGT was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-OGT Antibody (PB9767) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9767-ogt-primary-antibodies-ihc-testing-4.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; IHC analysis of OGT using anti-OGT antibody (PB9767). <br> OGT was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OGT Antibody (PB9767) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9767-ogt-primary-antibodies-ihc-testing-5.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; IHC analysis of OGT using anti-OGT antibody (PB9767). <br> OGT was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OGT Antibody (PB9767) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9767-ogt-primary-antibodies-ihc-testing-6.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; IHC analysis of OGT using anti-OGT antibody (PB9767). <br> OGT was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-OGT Antibody (PB9767) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9767-ogt-primary-antibodies-if-testing-7.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; IF analysis of OGT using anti-OGT antibody (PB9767). <br> OGT was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-OGT Antibody (PB9767) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9767-ogt-primary-antibodies-fcm-testing-8.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-OGT antibody (PB9767).<br>Overlay histogram showing U937 cells stained with PB9767 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGT Antibody (PB9767,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9767-ogt-primary-antibodies-fcm-testing-9.jpgAnti-OGT/O-Linked N-Acetylglucosamine Transferase Antibody Picoband&reg; Flow Cytometry analysis of RAW264.7 cells using anti-OGT antibody (PB9767).<br>Overlay histogram showing RAW264.7 cells stained with PB9767 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OGT Antibody (PB9767,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pax6-picoband-trade-antibody-pb9768-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9768-1-WB-anti-pax6-picoband-antibody.jpgAnti-PAX6 Antibody Picoband&reg; Western blot analysis of PAX6 using anti-PAX6 antibody (PB9768). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: U87 Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6 antigen affinity purified polyclonal antibody (Catalog # PB9768) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX6 at approximately 47 kDa. The expected band size for PAX6 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9768-2-IHC-anti-pax6-picoband-antibody.jpgAnti-PAX6 Antibody Picoband&reg; IHC analysis of PAX6 using anti-PAX6 antibody (PB9768). <br> PAX6 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PAX6 Antibody (PB9768) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9768-ijo-18-04-582-g002.jpgAnti-PAX6 Antibody Picoband&reg;Morphology and identification of OC-RSCs. A: Passage 0; B: Passage 3; A and B: bar=100 µm; C: Co-expression of CHX10 and BrdU markers; D: Co-expression of PAX6 and BrdU markers, C and D, bar=20 µm. CHX10: Ceh Homeobox 10; PAX6: Paired box 6; BrdU: Bromodeoxyuridine; OC-RSC: Optic-cup-derived retinal stem cells; DAPI: 4′,6-diamidino-2-phenylindole.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11947540/'>40256021</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9768-3-IHC-anti-pax6-picoband-antibody.jpgAnti-PAX6 Antibody Picoband&reg; IHC analysis of PAX6 using anti-PAX6 antibody (PB9768). <br> PAX6 was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PAX6 Antibody (PB9768) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9768-4-IHC-anti-pax6-picoband-antibody.jpgAnti-PAX6 Antibody Picoband&reg; IHC analysis of PAX6 using anti-PAX6 antibody (PB9768). <br> PAX6 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PAX6 Antibody (PB9768) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pace4-picoband-trade-antibody-pb9769-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9769-1-WB-anti-pace4-picoband-antibody.jpgAnti-PACE4/PCSK6 Antibody Picoband&reg; Western blot analysis of PACE4 using anti-PACE4 antibody (PB9769). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: 293T Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PACE4 antigen affinity purified polyclonal antibody (Catalog # PB9769) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PACE4 at approximately 80 kDa, 45/80/100 kDa. The expected band size for PACE4 is at 80 kDa, 45/80/100 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alix-picoband-trade-antibody-pb9770-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9770-alix-primary-antibodies-wb-testing-1_1.jpgAnti-ALIX/PDCD6IP Antibody Picoband&reg; Western blot analysis of ALIX using anti-ALIX antibody (PB9770). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: rat liver tissue lysates,<br> Lane 4: rat RH35 whole cell lysates,<br> Lane 5: mouse liver tissue lysates,<br> Lane 6: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALIX antigen affinity purified polyclonal antibody (Catalog # PB9770) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ALIX at approximately 96 kDa. The expected band size for ALIX is at 96 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9770-alix-primary-antibodies-ihc-testing-2.jpgAnti-ALIX/PDCD6IP Antibody Picoband&reg; IHC analysis of ALIX using anti-ALIX antibody (PB9770). <br> ALIX was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALIX Antibody (PB9770) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9770-alix-primary-antibodies-if-testing-3.jpgAnti-ALIX/PDCD6IP Antibody Picoband&reg; IF analysis of ALIX using anti-ALIX antibody (PB9770). <br> ALIX was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALIX Antibody (PB9770) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9770-alix-primary-antibodies-if-testing-4.jpgAnti-ALIX/PDCD6IP Antibody Picoband&reg; IF analysis of ALIX using anti-ALIX antibody (PB9770). <br> ALIX was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ALIX Antibody (PB9770) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9770-alix-primary-antibodies-if-testing-5.jpgAnti-ALIX/PDCD6IP Antibody Picoband&reg; IF analysis of ALIX using anti-ALIX antibody (PB9770). <br> ALIX was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ALIX Antibody (PB9770) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9770-alix-primary-antibodies-fcm-testing-6.jpgAnti-ALIX/PDCD6IP Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-ALIX antibody (PB9770). <br> Overlay histogram showing Jurkat cells stained with PB9770 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALIX Antibody (PB9770, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdgfra-picoband-trade-antibody-pb9771-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9771-1_1.jpgAnti-PDGF Receptor alpha/PDGFRA Antibody Picoband&reg; Western blot analysis of PDGFRA using anti-PDGFRA antibody (PB9771).<br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HT1080 whole cell lysates&#44;<br> Lane 2: human Caco-2 whole cell lysates&#44;<br> Lane 3: human U20S whole cell lysates&#44; <br> Lane 4: human PC-3 whole cell lysates&#44;<br> Lane 5: human 293T whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDGFRA antigen affinity purified polyclonal antibody (Catalog # PB9771) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDGFRA at approximately 123KD. The expected band size for PDGFRA is at 180KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9771-2-IHC-anti-pdgfra-pdgf-receptor-alpha-picoband-antibody.jpgAnti-PDGF Receptor alpha/PDGFRA Antibody Picoband&reg; IHC analysis of PDGFRA using anti-PDGFRA antibody (PB9771). <br> PDGFRA was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PDGFRA Antibody (PB9771) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9771-ihc-2.jpgAnti-PDGF Receptor alpha/PDGFRA Antibody Picoband&reg;IHC analysis of PDGFRA using anti-PDGFRA antibody (PB9771).<br> PDGFRA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDGFRA Antibody (PB9771) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9771-ihc-3.jpgAnti-PDGF Receptor alpha/PDGFRA Antibody Picoband&reg;IHC analysis of PDGFRA using anti-PDGFRA antibody (PB9771).<br> PDGFRA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDGFRA Antibody (PB9771) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9771-ihc-4.jpgAnti-PDGF Receptor alpha/PDGFRA Antibody Picoband&reg;IHC analysis of PDGFRA using anti-PDGFRA antibody (PB9771).<br> PDGFRA was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PDGFRA Antibody (PB9771) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-erp57-picoband-trade-antibody-pb9772-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9772-1-WB-anti-erp57-picoband-antibody.jpgAnti-ERp57/PDIA3 Antibody Picoband&reg; Western blot analysis of ERp57 using anti-ERp57 antibody (PB9772). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate&#44; Lane 2: Mouse Liver Tissue Lysate&#44; Lane 3: A549 Whole Cell Lysate. After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERp57 antigen affinity purified polyclonal antibody (Catalog # PB9772) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERp57 at approximately 57KD. The expected band size for ERp57 is at 57KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9772-2-IHC-anti-erp57-picoband-antibody.jpgAnti-ERp57/PDIA3 Antibody Picoband&reg; IHC analysis of PB9771 using anti-PB9771 antibody (PB9772). PB9771 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PB9771 Antibody (PB9772) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9772-erp57-primary-antibodies-ihc-testing-3.jpgAnti-ERp57/PDIA3 Antibody Picoband&reg; IHC analysis of ERp57 using anti-ERp57 antibody (PB9772).<br> ERp57 was detected in frozen section of human placenta tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ERp57 Antibody (PB9772) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9772-erp57-primary-antibodies-if-testing-4.jpgAnti-ERp57/PDIA3 Antibody Picoband&reg; IF analysis of ERp57 using anti-ERp57 antibody (PB9772). <br> ERp57 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ERp57 Antibody (PB9772) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9772-erp57-primary-antibodies-fcm-testing-5.jpgAnti-ERp57/PDIA3 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-ERp57 antibody (PB9772). <br>Overlay histogram showing PC-3 cells stained with PB9772 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ERp57 Antibody (PB9772, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pdk4-picoband-trade-antibody-pb9773-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9773-41467_2024_55418_fig6_html.pngAnti-PDK4 Antibody Picoband&reg;AMPK activity, protein expression and phosphorylation of PDH and PDK4 before and after fasting. Effect of seven days’ fasting on activity, phosphorylation and expression of selected proteins. White and grey bars represent means before and after seven days of fasting. White circles represent individuals before fasting and black after fasting, with dotted lines to illustrate individual change. A , B and C Activity of AMPK complex: α1β2γ1, α2β2γ1, and α2β2γ3. D Protein expression of PDH. E Phosphorylation of PDH at site 1. F Phosphorylation of PDH at site 2. G Expression of PDK4. Bars show means. Two-sided paired t tests were used for comparisons. N = 13 for (A – C , F and G ) N = 12 for ( D and E ). Abbreviation: AMPK: 5’-Adenosine monophosphate-activated protein kinase. PDH: pyruvate dehydrogenase, PDK4: pyruvate dehydrogenase kinase-4. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-024-55418-0'>39747857</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9773-pdk4-primary-antibodies-wb-testing-1.jpgAnti-PDK4 Antibody Picoband&reg; Western blot analysis of PDK4 using anti-PDK4 antibody (PB9773). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: rat skeletal muscle tissue lysates, <br> Lane 5: rat heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDK4 antigen affinity purified polyclonal antibody (Catalog # PB9773) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PDK4 at approximately 46 kDa. The expected band size for PDK4 is at 46 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pgk1-picoband-trade-antibody-pb9774-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9774-pgk1-primary-antibodies-wb-testing-1.jpgAnti-PGK1 Antibody Picoband&reg; Western blot analysis of PGK1 using anti-PGK1 antibody (PB9774). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HepG2 whole cell lysates, <br> Lane 3: human 293T whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: mouse brain tissue lysates, <br> Lane 7: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGK1 antigen affinity purified polyclonal antibody (Catalog # PB9774) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGK1 at approximately 43 kDa. The expected band size for PGK1 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9774-pgk1-primary-antibodies-if-testing-2.jpgAnti-PGK1 Antibody Picoband&reg; IF analysis of PGK1 using anti-PGK1 antibody (PB9774). <br> PGK1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PGK1 Antibody (PB9774) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9774-pgk1-primary-antibodies-fcm-testing-3.jpgAnti-PGK1 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-PGK1 antibody (PB9774). <br>Overlay histogram showing 293T cells stained with PB9774 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGK1 Antibody (PB9774, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pgrmc1-picoband-trade-antibody-pb9775-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9775-pgrmc1-primary-antibodies-wb-testing-1.jpgAnti-PGRMC1 Antibody Picoband&reg;Western blot analysis of PGRMC1 using anti-PGRMC1 antibody (PB9775). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human U251 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGRMC1 antigen affinity purified polyclonal antibody (PB9775) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PGRMC1 at approximately 22 kDa. The expected band size for PGRMC1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9775-pgrmc1-primary-antibodies-ihc-testing-1.jpgAnti-PGRMC1 Antibody Picoband&reg;IHC analysis of PGRMC1 using anti-PGRMC1 antibody (PB9775). <br>PGRMC1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGRMC1 Antibody (PB9775) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9775-pgrmc1-primary-antibodies-ihc-testing-2.jpgAnti-PGRMC1 Antibody Picoband&reg;IHC analysis of PGRMC1 using anti-PGRMC1 antibody (PB9775). <br>PGRMC1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PGRMC1 Antibody (PB9775) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9775-pgrmc1-primary-antibodies-if-testing-1.jpgAnti-PGRMC1 Antibody Picoband&reg;IF analysis of PGRMC1 using anti-PGRMC1 antibody (PB9775). <br> PGRMC1 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PGRMC1 Antibody (PB9775) overnight at 4°C. Cy Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pigr-picoband-trade-antibody-pb9776-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9776-pigr-primary-antibodies-wb-testing-1.jpgAnti-PIGR Antibody Picoband&reg;Western blot analysis of PIGR using anti-PIGR antibody (PB9776). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human SH-SY5Y whole cell lysates,<br> Lane 3: human RT4 whole cell lysates,<br> Lane 4: human CT26WT whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIGR antigen affinity purified polyclonal antibody (Catalog # PB9776) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIGR at approximately 85 kDa. The expected band size for PIGR is at 85 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9776-pigr-primary-antibodies-fcm-testing-1.jpgAnti-PIGR Antibody Picoband&reg;Flow Cytometry analysis of SH-SY5Y cells using anti-PIGR antibody (PB9776). <br> Overlay histogram showing SH-SY5Y cells stained with PB9776 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PIGR Antibody (PB9776, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pik3r2-picoband-trade-antibody-pb9777-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9777-pik3r2-primary-antibodies-wb-testing-1.jpgAnti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband&reg;Western blot analysis of PIK3R2 using anti-PIK3R2 antibody (PB9777). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3R2 antigen affinity purified polyclonal antibody (Catalog # PB9777) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PIK3R2 at approximately 82 kDa. The expected band size for PIK3R2 is at 82 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9777-pik3r2-primary-antibodies-fcm-testing-1.jpgAnti-PI 3 Kinase p85 beta/PIK3R2 Antibody Picoband&reg;Flow Cytometry analysis of THP-1 cells using anti-PIK3R2 antibody (PB9777). <br> Overlay histogram showing THP-1 cells stained with PB9777 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PIK3R2 Antibody (PB9777, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pla2g4a-picoband-trade-antibody-pb9778-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9778-1-WB-anti-pla2g4a-picoband-antibody.jpgAnti-Phospholipase A2/PLA2G4A Antibody Picoband&reg; Western blot analysis of PLA2G4A using anti-PLA2G4A antibody (PB9778). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: NIH/3T3 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLA2G4A antigen affinity purified polyclonal antibody (Catalog # PB9778) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLA2G4A at approximately 85 kDa. The expected band size for PLA2G4A is at 85 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pon2-picoband-trade-antibody-pb9779-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9779-1-WB-anti-pon2-picoband-antibody.jpgAnti-PON2 Antibody Picoband&reg; Western blot analysis of PON2 using anti-PON2 antibody (PB9779). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON2 antigen affinity purified polyclonal antibody (Catalog # PB9779) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PON2 at approximately 39 kDa. The expected band size for PON2 is at 39 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pot1-picoband-trade-antibody-pb9780-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9780-pot1-primary-antibodies-wb-testing-1.jpgAnti-POT1 Antibody Picoband&reg; Western blot analysis of POT1 using anti-POT1 antibody (PB9780). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: rat liver tissue lysates,<br> Lane 5: mouse Neuro-2a whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POT1 antigen affinity purified polyclonal antibody (Catalog # PB9780) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POT1 at approximately 71KD. The expected band size for POT1 is at 71KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9780-pot1-primary-antibodies-fcm-testing-2.jpgAnti-POT1 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-POT1 antibody (PB9780).<br>Overlay histogram showing U937 cells stained with PB9780 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POT1 Antibody (PB9780,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ppt1-picoband-trade-antibody-pb9781-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9781-1_2-WB-anti-ppt1-cln1-picoband-antibody.jpgAnti-PPT1 Antibody Picoband&reg;<b> Western blot analysis of PPT1 using anti-PPT1 antibody (PB9781). </b><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: mouse brain tissue lysates&#44; <br> Lane 3: rat liver tissue lysates&#44;<br> Lane 4: mouse liver tissue lysates&#44;<br> Lane 5: HEPG2 whole Cell lysates&#44; <br> Lane 6: 293T whole cell lysates&#44; <br> Lane 7: MCF-7 whole cell lysates.<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPT1 antigen affinity purified polyclonal antibody (Catalog # PB9781) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPT1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9781-2-IHC-anti-ppt1-cln1-picoband-antibody.jpgAnti-PPT1 Antibody Picoband&reg;<b> IHC analysis of PPT1 using anti-PPT1 antibody (PB9781).</b><br> PPT1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PPT1 Antibody (PB9781) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9781-ppt1-primary-antibodies-fc-testing-3.jpgAnti-PPT1 Antibody Picoband&reg;<b> Flow Cytometry analysis of U937 cells using anti-PPT1 antibody (PB9781).</b><br>Overlay histogram showing U937 cells stained with PB9781 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPT1 Antibody (PB9781&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9781-ppt1-primary-antibodies-fc-testing-4.jpgAnti-PPT1 Antibody Picoband&reg;<b> Flow Cytometry analysis of SiHa cells using anti-PPT1 antibody (PB9781).</b><br>Overlay histogram showing SiHa cells stained with PB9781 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPT1 Antibody (PB9781&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9781-ppt1-primary-antibodies-fc-testing-5.jpgAnti-PPT1 Antibody Picoband&reg;<b> Flow Cytometry analysis of THP-1 cells using anti-PPT1 antibody (PB9781).</b><br>Overlay histogram showing THP-1 cells stained with PB9781 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPT1 Antibody (PB9781&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prlr-picoband-trade-antibody-pb9782-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9782-prlr-primary-antibodies-wb-testing-1.jpgAnti-Prolactin Receptor/PRLR Antibody Picoband&reg; Western blot analysis of PRLR using anti-PRLR antibody (PB9782). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRLR antigen affinity purified polyclonal antibody (Catalog # PB9782) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRLR at approximately 65, 95 kDa. The expected band size for PRLR is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prnp-picoband-trade-antibody-pb9783-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9783-1-WB-anti-prnp-prp-picoband-antibody.jpgAnti-Prion protein PrP/PRNP Antibody Picoband&reg; Western blot analysis of PRNP using anti-PRNP antibody (PB9783). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.<br> Lane 1: Rat Brain Tissue Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRNP antigen affinity purified polyclonal antibody (Catalog # PB9783) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRNP at approximately 30 kDa. The expected band size for PRNP is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9783-2-IHC-anti-prnp-prp-picoband-antibody.jpgAnti-Prion protein PrP/PRNP Antibody Picoband&reg; IHC analysis of PRNP using anti-PRNP antibody (PB9783). <br> PRNP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRNP Antibody (PB9783) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9783-3-IHC-anti-prnp-prp-picoband-antibody.jpgAnti-Prion protein PrP/PRNP Antibody Picoband&reg; IHC analysis of PRNP using anti-PRNP antibody (PB9783). <br> PRNP was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PRNP Antibody (PB9783) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd45-picoband-trade-antibody-pb9785-boster.html2026-03-24T05:05:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9785-cd45-primary-antibodies-wb-testing-1.jpgAnti-CD45/PTPRC Antibody Picoband&reg; Western blot analysis of CD45 using anti-CD45 antibody (PB9785). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD45 antigen affinity purified polyclonal antibody (Catalog # PB9785) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD45 at approximately 180-250 kDa. The expected band size for CD45 is at 147 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9785-cd45-primary-antibodies-ihc-testing-2.jpgAnti-CD45/PTPRC Antibody Picoband&reg; IHC analysis of CD45 using anti-CD45 antibody (PB9785). <br> CD45 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD45 Antibody (PB9785) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9785-cd45-primary-antibodies-if-testing-3.jpgAnti-CD45/PTPRC Antibody Picoband&reg; IF analysis of CD45 using anti-CD45 antibody (PB9785). <br> CD45 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD45 Antibody (PB9785) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9785-cd45-primary-antibodies-fcm-testing-4_1.jpgAnti-CD45/PTPRC Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-CD45 antibody (PB9785). <br> Overlay histogram showing Jurkat cells stained with PB9785 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD45 Antibody (PB9785, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lar-picoband-trade-antibody-pb9786-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9786-1_1-WB-anti-lar-picoband-antibody.jpgAnti-LAR/PTPRF Antibody Picoband&reg; Western blot analysis of LAR using anti-LAR antibody (PB9786). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: A431 Whole Cell Lysate,<br> Lane 3: A549 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAR antigen affinity purified polyclonal antibody (Catalog # PB9786) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LAR at approximately 240 kDa. The expected band size for LAR is at 240 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9786-2-IHC-anti-lar-picoband-antibody.jpgAnti-LAR/PTPRF Antibody Picoband&reg; IHC analysis of LAR using anti-LAR antibody (PB9786). <br> LAR was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LAR Antibody (PB9786) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab5-picoband-trade-antibody-pb9787-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9787-rab5a-primary-antibodies-wb-testing-1.jpgAnti-Rab5/RAB5A Antibody Picoband&reg;Western blot analysis of RAB5A using anti-RAB5A antibody (PB9787). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human U251 whole cell lysates,<br> Lane 4: human PC-3 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5A antigen affinity purified polyclonal antibody (PB9787) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB5A at approximately 24 kDa. The expected band size for RAB5A is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9787-2-IHC-anti-rab5-picoband-antibody.jpgAnti-Rab5/RAB5A Antibody Picoband&reg; IHC analysis of Rab5 using anti-Rab5 antibody (PB9787). <br> Rab5 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Rab5 Antibody (PB9787) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab10-picoband-trade-antibody-pb9788-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9788-rab10-primary-antibodies-wb-testing-1.jpgAnti-RAB10 Antibody Picoband&reg; Western blot analysis of RAB10 using anti-RAB10 antibody (PB9788). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human A549 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat C6 whole cell lysates, <br> Lane 6: mouse brain tissue lysates, <br> Lane 7: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody (Catalog # PB9788) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB10 at approximately 23 kDa. The expected band size for RAB10 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab11a-picoband-trade-antibody-pb9789-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9789-rab11a-primary-antibodies-wb-testing-1.jpgAnti-Rab11A Antibody Picoband&reg;Western blot analysis of Rab11A using anti-Rab11A antibody (PB9789). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human SH-SY5Y whole cell lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates, <br> Lane 5: human A549 whole cell lysates, <br> Lane 6: human RT4 whole cell lysates, <br> Lane 7: human Hacat whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (PB9789) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9789-rab11a-primary-antibodies-wb-testing-2.jpgAnti-Rab11A Antibody Picoband&reg;Western blot analysis of Rab11A using anti-Rab11A antibody (PB9789). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat spleen tissue lysates, <br> Lane 3: rat lung tissue lysates, <br> Lane 4: rat PC-12 whole cell lysates, <br> Lane 5: mouse brain tissue lysates, <br> Lane 6: mouse spleen tissue lysates, <br> Lane 7: mouse lung tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab11A antigen affinity purified polyclonal antibody (PB9789) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Rab11A at approximately 24 kDa. The expected band size for Rab11A is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9789-rab11a-primary-antibodies-if-testing-1.jpgAnti-Rab11A Antibody Picoband&reg;IF analysis of Rab11A using anti-Rab11A antibody (PB9789). <br> Rab11A was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Rab11A Antibody (PB9789) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9789-rab11a-primary-antibodies-fcm-testing-1.jpgAnti-Rab11A Antibody Picoband&reg;Flow Cytometry analysis of SH-SY5Y cells using anti-Rab11A antibody (PB9789). <br> Overlay histogram showing SH-SY5Y cells stained with PB9789 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rab11A Antibody (PB9789, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab13-picoband-trade-antibody-pb9790-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9790-rab13-primary-antibodies-fcm-testing-3_1.jpgAnti-RAB13 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-RAB13 antibody (PB9790). <br>Overlay histogram showing U87 cells stained with PB9790 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB13 Antibody (PB9790, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9790-rab13-primary-antibodies-wb-testing-1_1.jpgAnti-RAB13 Antibody Picoband&reg; Western blot analysis of RAB13 using anti-RAB13 antibody (PB9790). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human CACO-2 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB13 antigen affinity purified polyclonal antibody (Catalog # PB9790) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB13 at approximately 23 kDa. The expected band size for RAB13 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9790-rab13-primary-antibodies-if-testing-2.jpgAnti-RAB13 Antibody Picoband&reg; IF analysis of RAB13 using anti-RAB13 antibody (PB9790). <br> RAB13 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB13 Antibody (PB9790) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab18-picoband-trade-antibody-pb9791-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9791-1-WB-anti-rab18-picoband-antibody.jpgAnti-Rab18 Antibody Picoband&reg; Western blot analysis of Rab18 using anti-Rab18 antibody (PB9791). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Testis Tissue Lysate at 50ug,<br> Lane 2: Rat Lung Tissue Lysate at 50ug,<br> Lane 3: 293T Whole Cell Lysate at 40ug,<br> Lane 4: HELA Whole Cell Lysate at 40ug,<br> Lane 5: HEPG2 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab18 antigen affinity purified polyclonal antibody (Catalog # PB9791) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab18 at approximately 23 kDa. The expected band size for Rab18 is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rad51c-picoband-trade-antibody-pb9792-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9792-rad51c-primary-antibodies-wb-testing-1_1.jpgAnti-Rad51C Antibody Picoband&reg; Western blot analysis of Rad51C using anti-Rad51C antibody (PB9792). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rad51C antigen affinity purified polyclonal antibody (Catalog # PB9792) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rad51C at approximately 42 kDa. The expected band size for Rad51C is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rag2-picoband-trade-antibody-pb9793-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9793-1-WB-anti-rag2-picoband-antibody.jpgAnti-RAG2 Antibody Picoband&reg; Western blot analysis of RAG2 using anti-RAG2 antibody (PB9793). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: A549 Whole Cell Lysate,<br> Lane 2: 22RV1 Whole Cell Lysate,<br> Lane 3: U20S Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAG2 antigen affinity purified polyclonal antibody (Catalog # PB9793) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAG2 at approximately 59 kDa. The expected band size for RAG2 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9793-2-IHC-anti-rag2-picoband-antibody.jpgAnti-RAG2 Antibody Picoband&reg; IHC analysis of RAG2 using anti-RAG2 antibody (PB9793). <br> RAG2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RAG2 Antibody (PB9793) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9793-3-IHC-anti-rag2-picoband-antibody.jpgAnti-RAG2 Antibody Picoband&reg; IHC analysis of RAG2 using anti-RAG2 antibody (PB9793). <br> RAG2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RAG2 Antibody (PB9793) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9793-4-IHC-anti-rag2-picoband-antibody.jpgAnti-RAG2 Antibody Picoband&reg; IHC analysis of RAG2 using anti-RAG2 antibody (PB9793). <br> RAG2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RAG2 Antibody (PB9793) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rala-picoband-trade-antibody-pb9794-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9794-rala-primary-antibodies-wb-testing-1.jpgAnti-RALA Antibody Picoband&reg; Western blot analysis of RALA using anti-RALA antibody (PB9794). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human SW620 whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: rat brain tissue lysates,<br> Lane 7: mouse lung tissue lysates,<br> Lane 8: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALA antigen affinity purified polyclonal antibody (Catalog # PB9794) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALA at approximately 24 kDa. The expected band size for RALA is at 24 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ralb-picoband-trade-antibody-pb9795-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9795-1-WB-anti-ralb-picoband-antibody.jpgAnti-RALB Antibody Picoband&reg; Western blot analysis of RALB using anti-RALB antibody (PB9795). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Rat Thymus Tissue Lysate at 50ug,<br> Lane 3: Rat Lung Tissue Lysate at 50ug,<br> Lane 4: Mouse Spleen Tissue Lysate at 50ug,<br> Lane 5: Mouse Liver Tissue Lysate at 50ug,<br> Lane 6: HELA Whole Cell Lysate at 40ug,<br> Lane 7: 22RV1 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RALB antigen affinity purified polyclonal antibody (Catalog # PB9795) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RALB at approximately 23 kDa. The expected band size for RALB is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9795-ralb-primary-antibodies-if-testing-2.jpgAnti-RALB Antibody Picoband&reg; IF analysis of RALB using anti-RALB antibody (PB9795). <br> RALB was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-RALB Antibody (PB9795) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9795-ralb-primary-antibodies-fcm-testing-3.pngAnti-RALB Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-RALB antibody (PB9795). <br>Overlay histogram showing Jurkat cells stained with PB9795 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-RALB Antibody (PB9795, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rasa1-picoband-trade-antibody-pb9796-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9796-1-WB-anti-rasa1-ras-gap-picoband-antibody.jpgAnti-RASA1 Antibody Picoband&reg; Western blot analysis of RASA1 using anti-RASA1 antibody (PB9796). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: MCF-7 Whole Cell Lysate,<br> Lane 3: SMMC Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASA1 antigen affinity purified polyclonal antibody (Catalog # PB9796) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASA1 at approximately 100 kDa. The expected band size for RASA1 is at 100 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9796-rasa1-primary-antibodies-if-testing-2.jpgAnti-RASA1 Antibody Picoband&reg; IF analysis of RASA1 using anti-RASA1 antibody (PB9796). <br> RASA1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RASA1 Antibody (PB9796) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9796-rasa1-primary-antibodies-fcm-testing-3.jpgAnti-RASA1 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-RASA1 antibody (PB9796).<br>Overlay histogram showing U87 cells stained with PB9796 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASA1 Antibody (PB9796,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rbap48-picoband-trade-antibody-pb9797-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-wb-testing-1.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; Western blot analysis of RbAp48 using anti-RbAp48 antibody (PB9797). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Raji whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human U87 whole cell lysates,<br> Lane 5: human HepG2 whole cell lysates,<br> Lane 6: human K562 whole cell lysates,<br> Lane 7: human RT4 whole cell lysates,<br> Lane 8: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RbAp48 antigen affinity purified polyclonal antibody (Catalog # PB9797) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RbAp48 at approximately 50-55 kDa. The expected band size for RbAp48 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-wb-testing-2.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; Western blot analysis of RbAp48 using anti-RbAp48 antibody (PB9797). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat RH35 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates,<br> Lane 7: mouse lung tissue lysates,<br> Lane 8: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RbAp48 antigen affinity purified polyclonal antibody (Catalog # PB9797) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RbAp48 at approximately 50-55 kDa. The expected band size for RbAp48 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-wb-testing-3.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; Western blot analysis of RbAp48 using anti-RbAp48 antibody (PB9797). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat liver tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: rat RH35 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse lung tissue lysates,<br> Lane 9: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RbAp48 antigen affinity purified polyclonal antibody (Catalog # PB9797) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for RbAp48 at approximately 50-55 kDa. The expected band size for RbAp48 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9797-4_1-IHC-anti-rbap48-picoband-antibody.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797). RbAp48 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-5-ihc-anti-rbap48-picoband-antibody.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797). RbAp48 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-ihc-testing-6.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).<br> RbAp48 was detected in frozen section of mouse liver tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-ihc-testing-7.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).<br> RbAp48 was detected in frozen section of mouse small intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-ihc-testing-8.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).<br> RbAp48 was detected in frozen section of rat small intestine tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-ihc-testing-9.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797).<br> RbAp48 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-10_1-ihc-anti-rbap48-picoband-antibody.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IHC analysis of RbAp48 using anti-RbAp48 antibody (PB9797). RbAp48 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-if-testing-11.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IF analysis of RbAp48 using anti-RbAp48 antibody (PB9797) and anti-Beta Tubulin antibody (M01857-3).<br> RbAp48 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-RbAp48 Antibody (PB9797) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-if-testing-12.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; IF analysis of RbAp48 using anti-RbAp48 antibody (PB9797). <br> RbAp48 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RbAp48 Antibody (PB9797) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9797-rbap48-primary-antibodies-fcm-testing-13.jpgAnti-RbAp48/RBBP4 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-RbAp48 antibody (PB9797).<br>Overlay histogram showing 293T cells stained with PB9797 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RbAp48 Antibody (PB9797,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-roc1-picoband-trade-antibody-pb9798-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9798-1-WB-anti-roc1-picoband-antibody.jpgAnti-ROC1/RBX1 Antibody Picoband&reg; Western blot analysis of ROC1 using anti-ROC1 antibody (PB9798). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.<br> Lane 1: Rat Testis Tissue Lysate,<br> Lane 2: Rat Brain Tissue Lysate,<br> Lane 3: Mouse Brain Tissue Lysate,<br> Lane 4: Mouse Spleen Tissue Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROC1 antigen affinity purified polyclonal antibody (Catalog # PB9798) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROC1 at approximately 15 kDa. The expected band size for ROC1 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9798-2.pngAnti-ROC1/RBX1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ROC1 antibody (PB9798). <br> Overlay histogram showing A431 cells stained with PB9798 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROC1 Antibody (PB9798&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9798-3.jpgAnti-ROC1/RBX1 Antibody Picoband&reg; IF analysis of ROC1 using anti-ROC1 antibody (PB9798). <br> ROC1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ROC1 Antibody (PB9798) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rel-b-picoband-trade-antibody-pb9799-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9799-relb-primary-antibodies-wb-testing-1.jpgAnti-Rel B/RELB Antibody Picoband&reg; Western blot analysis of Rel B using anti-Rel B antibody (PB9799). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Ramos whole cell lysates,<br> Lane 2: human Daudi whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rel B antigen affinity purified polyclonal antibody (Catalog # PB9799) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rel B at approximately 65-70 kDa. The expected band size for Rel B is at 62 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gtpase-hras-antibody-rp1098-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1098-1-WB-anti-gtpase-hras-antibody.jpgAnti-GTPase HRAS Antibody Picoband&reg;Anti-GTPase HRAS antibody&#44; RP1098&#44; Western blotting<br>All lanes: Anti GTPase HRAS (RP1098) at 0.5ug/ml<br> Lane 1: Mouse Brain Tissue Lysate at 50ug<br> Lane 2: HELA Whole Cell Lysate at 40ug<br> Lane 3: A549 Whole Cell Lysate at 40ug<br> Predicted bind size: 21KD<br> Observed bind size: 21KD https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gtpase-hras-antibody-rp1099-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1099-1_3-WB-anti-gtpase-hras-antibody.jpgAnti-GTPase HRAS Antibody Picoband&reg;Anti-GTPase HRAS antibody&#44; RP1099&#44; Western blotting<br>All lanes: Anti GTPase HRAS (RP1099) at 0.5ug/ml<br>Lane 1: Rat Brain Tissue Lysate at 50ug<br>Lane 2: Mouse Brain Tissue Lysate at 50ug<br>Lane 3: HEPA Whole Cell Lysate at 40ug<br>Predicted bind size: 21KD<br>Observed bind size: 21KDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1099-hras-primary-antibodies-ihc-testing-3.jpgAnti-GTPase HRAS Antibody Picoband&reg;IHC analysis of HRAS using anti-HRAS antibody (RP1099). <br>HRAS was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-HRAS Antibody (RP1099) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1099-2.jpgAnti-GTPase HRAS Antibody Picoband&reg; IHC analysis of GTPase HRAS using anti-GTPase HRAS antibody (RP1099). <br> GTPase HRAS was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GTPase HRAS Antibody (RP1099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd19-picoband-trade-antibody-pb9800-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9800-cd19-primary-antibodies-wb-testing-1.jpgAnti-CD19 Antibody Picoband&reg;Western blot analysis of CD19 using anti-CD19 antibody (PB9800). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates, <br> Lane 2: human Ramos whole cell lysates, <br> Lane 3: human Daudi whole cell lysates, <br> Lane 4: human Jurkat whole cell lysates (negative control). <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (PB9800) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 61 kDa.|IHC analysis of CD19 using anti-CD19 antibody (PB9800). <br> CD19 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD19 Antibody (PB9800) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9800-cd19-primary-antibodies-ihc-testing-2.jpgAnti-CD19 Antibody Picoband&reg; IHC analysis of CD19 using anti-CD19 antibody (PB9800). <br> CD19 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD19 Antibody (PB9800) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9800-cd19-primary-antibodies-if-testing-1.jpgAnti-CD19 Antibody Picoband&reg;IF analysis of CD19 using anti-CD19 antibody (PB9800). <br> CD19 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD19 Antibody (PB9800) overnight at 4°C. Fluoro594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dgat1-picoband-trade-antibody-pb9801-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9801-1-WB-anti-dgat1-picoband-antibody.jpgAnti-DGAT1 Antibody Picoband&reg; Western blot analysis of DGAT1 using anti-DGAT1 antibody (PB9801). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Kidney Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DGAT1 antigen affinity purified polyclonal antibody (Catalog # PB9801) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DGAT1 at approximately 60 kDa. The expected band size for DGAT1 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psd95-picoband-trade-antibody-pb9802-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9802-1_1.jpgAnti-PSD95/DLG4 Antibody Picoband&reg; Western blot analysis of PSD95 using anti-PSD95 antibody (PB9802). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44;<br> Lane 2: mouse brain Tissue Lysate&#44;<br> Lane 3: human SHG-44 Whole Cell Lysate&#44;<br> Lane 4: human U-87MG Whole Cell Lysate<br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD95 antigen affinity purified polyclonal antibody (Catalog # PB9802) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD95 at approximately 100KD. The expected band size for PSD95 is at 80KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9802-2.pngAnti-PSD95/DLG4 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-PSD95 antibody (PB9802). <br> Overlay histogram showing A431 cells stained with PB9802 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSD95 Antibody (PB9802&#44;1&mu;g/1x10⁶ cells) for 30 min at 20&deg;C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10&mu;g/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20&deg;C. Isotype control antibody (Green line) was rabbit IgG (1&mu;g/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pkr-picoband-trade-antibody-pb9803-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9803-pkr-primary-antibodies-wb-testing-1.jpgAnti-PKR/EIF2AK2 Antibody Picoband&reg; Western blot analysis of PKR/EIF2AK2 using anti-PKR/EIF2AK2 antibody (PB9803). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human K562 whole cell lysates,<br> Lane 5: human PANC-1 whole cell lysates,<br> Lane 6: human A431 whole cell lysates,<br> Lane 7: human U251 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKR/EIF2AK2 antigen affinity purified polyclonal antibody (Catalog # PB9803) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKR/EIF2AK2 at approximately 69 kDa. The expected band size for PKR/EIF2AK2 is at 62 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9803-pkr-primary-antibodies-fcm-testing-3.jpgAnti-PKR/EIF2AK2 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-PKR/EIF2AK2 antibody (PB9803). <br>Overlay histogram showing HEL cells stained with PB9803 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PKR/EIF2AK2 Antibody (PB9803, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9803-pkr-primary-antibodies-if-testing-2.jpgAnti-PKR/EIF2AK2 Antibody Picoband&reg; IF analysis of PKR/EIF2AK2 using anti-PKR/EIF2AK2 antibody (PB9803). <br> PKR/EIF2AK2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-PKR/EIF2AK2 Antibody (PB9803) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eme1-picoband-trade-antibody-pb9804-boster.html2026-03-24T05:05:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9804-1-WB-anti-eme1-picoband-antibody.jpgAnti-EME1 Antibody Picoband&reg; Western blot analysis of EME1 using anti-EME1 antibody (PB9804). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: HELA Whole Cell Lysate&#44; <br> Lane 2: JURKAT Whole Cell Lysate&#44; <br> Lane 3: HUT Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EME1 antigen affinity purified polyclonal antibody (Catalog # PB9804) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EME1 at approximately 62KD. The expected band size for EME1 is at 62KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9804-2-IHC-anti-eme1-picoband-antibody.jpgAnti-EME1 Antibody Picoband&reg; IHC analysis of EME1 using anti-EME1 antibody (PB9804). <br> EME1 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EME1 Antibody (PB9804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9804-3-IHC-anti-eme1-picoband-antibody.jpgAnti-EME1 Antibody Picoband&reg; IHC analysis of EME1 using anti-EME1 antibody (PB9804). <br> EME1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EME1 Antibody (PB9804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9804-4.pngAnti-EME1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-EME1 antibody (PB9804). <br> Overlay histogram showing U20S cells stained with PB9804 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EME1 Antibody (PB9804&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-foxa3-picoband-trade-antibody-pb9805-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9805-foxa3-primary-antibodies-wb-testing-1.jpgAnti-FOXA3 Antibody Picoband&reg; Western blot analysis of FOXA3 using anti-FOXA3 antibody (PB9805). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA3 antigen affinity purified polyclonal antibody (Catalog # PB9805) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA3 at approximately 40 kDa. The expected band size for FOXA3 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9805-foxa3-primary-antibodies-ihc-testing-2.jpgAnti-FOXA3 Antibody Picoband&reg; IHC analysis of FOXA3 using anti-FOXA3 antibody (PB9805). <br> FOXA3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXA3 Antibody (PB9805) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9805-foxa3-primary-antibodies-ihc-testing-3.jpgAnti-FOXA3 Antibody Picoband&reg; IHC analysis of FOXA3 using anti-FOXA3 antibody (PB9805). <br> FOXA3 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXA3 Antibody (PB9805) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9805-foxa3-primary-antibodies-ihc-testing-4.jpgAnti-FOXA3 Antibody Picoband&reg; IHC analysis of FOXA3 using anti-FOXA3 antibody (PB9805). <br> FOXA3 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXA3 Antibody (PB9805) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9805-foxa3-primary-antibodies-ihc-testing-5.jpgAnti-FOXA3 Antibody Picoband&reg; IHC analysis of FOXA3 using anti-FOXA3 antibody (PB9805). <br> FOXA3 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXA3 Antibody (PB9805) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9805-foxa3-primary-antibodies-if-testing-6.jpgAnti-FOXA3 Antibody Picoband&reg; IF analysis of SDHA using anti-SDHA antibody (PB9433). <br> SDHA was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SDHA Antibody (PB9433) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9805-foxa3-primary-antibodies-fcm-testing-7.jpgAnti-FOXA3 Antibody Picoband&reg; Flow Cytometry analysis of 293T cells using anti-SDHA antibody (PB9433). <br> Overlay histogram showing 293T cells stained with PB9433 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SDHA Antibody (PB9433, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kv1-4-picoband-trade-antibody-pb9807-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9807-1-WB-anti-kv1-4-picoband-antibody.jpgAnti-Kv1.4/KCNA4 Antibody Picoband&reg; Western blot analysis of Kv1.4 using anti-Kv1.4 antibody (PB9807). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: COLO320 Whole Cell Lysate,<br> Lane 3: HT1080 Whole Cell Lysate,<br> Lane 4: PANC Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kv1.4 antigen affinity purified polyclonal antibody (Catalog # PB9807) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kv1.4 at approximately 73 kDa. The expected band size for Kv1.4 is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9807-2-IHC-anti-kv1-4-picoband-antibody.jpgAnti-Kv1.4/KCNA4 Antibody Picoband&reg; IHC analysis of Kv1.4 using anti-Kv1.4 antibody (PB9807). <br> Kv1.4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Kv1.4 Antibody (PB9807) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lck-picoband-trade-antibody-pb9808-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9808-lck-primary-antibodies-wb-testing-1.jpgAnti-Lck Antibody Picoband&reg; Western blot analysis of Lck using anti-Lck antibody (PB9808). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates, <br> Lane 2: mouse spleen tissue lysates, <br> Lane 3: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lck antigen affinity purified polyclonal antibody (Catalog # PB9808) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lck at approximately 58 kDa. The expected band size for Lck is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9808-2-IHC-anti-lck-picoband-antibody.jpgAnti-Lck Antibody Picoband&reg; IHC analysis of Lck using anti-Lck antibody (PB9808). <br> Lck was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lck Antibody (PB9808) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9808-3-IHC-anti-lck-picoband-antibody.jpgAnti-Lck Antibody Picoband&reg; IHC analysis of Lck using anti-Lck antibody (PB9808). <br> Lck was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lck Antibody (PB9808) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9808-4-IHC-anti-lck-picoband-antibody.jpgAnti-Lck Antibody Picoband&reg; IHC analysis of Lck using anti-Lck antibody (PB9808). <br> Lck was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Lck Antibody (PB9808) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9808-5.jpgAnti-Lck Antibody Picoband&reg; IHC analysis of Lck using anti-Lck antibody (PB9808). <br> Lck was detected in frozen section of mouse spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Lck Antibody (PB9808) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9808-6.jpgAnti-Lck Antibody Picoband&reg; IF analysis of Lck using anti-Lck antibody (PB9808). <br> Lck was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Lck Antibody (PB9808) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9808-7.jpgAnti-Lck Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Lck antibody (PB9808).<br>Overlay histogram showing HepG2 cells stained with PB9808 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Lck Antibody (PB9808,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aquaporin-0-picoband-trade-antibody-pb9811-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9811-1-WB-anti-aquaporin-0-picoband-antibody.jpgAnti-Aquaporin 0/MIP Antibody Picoband&reg; Western blot analysis of Aquaporin 0 using anti-Aquaporin 0 antibody (PB9811). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions.<br> Lane 1: rat eye ball tissue Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 0 antigen affinity purified polyclonal antibody (Catalog # PB9811) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 0 at approximately 28 kDa. The expected band size for Aquaporin 0 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-polb-picoband-trade-antibody-pb9812-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9812-polb-primary-antibodies-wb-testing-1.jpgAnti-DNA Polymerase beta/POLB Antibody Picoband&reg;Western blot analysis of POLB using anti-POLB antibody (PB9812). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat spleen tissue lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLB antigen affinity purified polyclonal antibody (PB9812) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for POLB at approximately 38 kDa. The expected band size for POLB is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-polh-picoband-trade-antibody-pb9813-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9813-1-WB-anti-polh-dna-polymerase-eta-picoband-antibody.jpgAnti-DNA polymerase eta/POLH Antibody Picoband&reg; Western blot analysis of POLH using anti-POLH antibody (PB9813). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Liver Tissue Lysate at 50ug,<br> Lane 2: HELA Whole Cell Lysate at 40ug,<br> Lane 3: SW620 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLH antigen affinity purified polyclonal antibody (Catalog # PB9813) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POLH at approximately 78 kDa. The expected band size for POLH is at 78 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prc1-picoband-trade-antibody-pb9814-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9814-prc1-primary-antibodies-wb-testing-1.jpgAnti-PRC1 Antibody Picoband&reg;Western blot analysis of PRC1 using anti-PRC1 antibody (PB9814). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human Hacat whole cell lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse testis tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 antigen affinity purified polyclonal antibody (PB9814) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PRC1 at approximately 72 kDa. The expected band size for PRC1 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9814-prc1-primary-antibodies-ip-testing-1.jpgAnti-PRC1 Antibody Picoband&reg;Immunoprecipitating (IP) PRC1 in 293T whole cell lysate.<br> Western blot analysis of PRC1 using anti-PRC1 antibody (PB9814); <br> Lane 1: 293T whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-PRC1 antibody in 293T whole cell lysate;<br> Lane 3: anti-PRC1 antibody (2μg) + 293T whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRC1 antigen affinity purified polyclonal antibody (PB9814) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for PRC1 at approximately 72 kDa. The expected band size for PRC1 is at 72 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9814-prc1-primary-antibodies-fcm-testing-1.jpgAnti-PRC1 Antibody Picoband&reg;Flow Cytometry analysis of 293T cells using anti-PRC1 antibody (PB9814). <br> Overlay histogram showing 293T cells stained with PB9814 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRC1 Antibody (PB9814, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab14-picoband-trade-antibody-pb9815-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-wb-testing-1.jpgAnti-RAB14 Antibody Picoband&reg;Western blot analysis of RAB14 using anti-RAB14 antibody (PB9815). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SiHa whole cell lysates,<br> Lane 3: human RT4 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB14 antigen affinity purified polyclonal antibody (PB9815) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB14 at approximately 24 kDa. The expected band size for RAB14 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-wb-testing-2.jpgAnti-RAB14 Antibody Picoband&reg;Western blot analysis of RAB14 using anti-RAB14 antibody (PB9815). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat PC-12 whole cell lysates,<br> Lane 3: mouse brain tissue lysates,<br> Lane 4: mouse stomach tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB14 antigen affinity purified polyclonal antibody (PB9815) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RAB14 at approximately 24 kDa. The expected band size for RAB14 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-ihc-testing-1.jpgAnti-RAB14 Antibody Picoband&reg;IHC analysis of RAB14 using anti-RAB14 antibody (PB9815). <br> RAB14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB14 Antibody (PB9815) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-ihc-testing-2.jpgAnti-RAB14 Antibody Picoband&reg;IHC analysis of RAB14 using anti-RAB14 antibody (PB9815). <br> RAB14 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB14 Antibody (PB9815) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-ihc-testing-3.jpgAnti-RAB14 Antibody Picoband&reg;IHC analysis of RAB14 using anti-RAB14 antibody (PB9815). <br> RAB14 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB14 Antibody (PB9815) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-ihc-testing-4.jpgAnti-RAB14 Antibody Picoband&reg;IHC analysis of RAB14 using anti-RAB14 antibody (PB9815). <br> RAB14 was detected in a paraffin-embedded section of human non-keratotic squamous cell carcinoma of the cervix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RAB14 Antibody (PB9815) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-if-testing-1.jpgAnti-RAB14 Antibody Picoband&reg;IF analysis of RAB14 using anti-RAB14 antibody (PB9815). <br> RAB14 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAB14 Antibody (PB9815) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9815-rab14-primary-antibodies-if-testing-2.jpgAnti-RAB14 Antibody Picoband&reg;IF analysis of RAB14 using anti-RAB14 antibody (PB9815. <br> RAB14was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-RAB14 Antibody (PB9815) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rap1a-picoband-trade-antibody-pb9816-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9816-1-WB-anti-rap1a-picoband-antibody.jpgAnti-RAP1A Antibody Picoband&reg; Western blot analysis of RAP1A using anti-RAP1A antibody (PB9816). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: Rat Brain Tissue Lysate&#44;<br> Lane 2: Rat Liver Tissue Lysate&#44;<br> Lane 3: Rat Kidney Tissue Lysate&#44;<br> Lane 4: MCF-7 Whole Cell Lysate&#44;<br> Lane 5: SW620 Whole Cell Lysate. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody (Catalog # PB9816) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9816-2_1.jpgAnti-RAP1A Antibody Picoband&reg; Western blot analysis of RAP1A using anti-RAP1A antibody (PB9816). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates&#44; <br> Lane 2: rat lung tissue lysates&#44; <br> Lane 3: rat stomach tissue lysates&#44; <br> Lane 4: rat kidney tissue lysates. <br> Lane 5: mouse heart tissue lysates&#44; <br> Lane 6: mouse lung tissue lysates&#44; <br> Lane 7: mouse stomach tissue lysates&#44; <br> Lane 8: mouse kidney tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAP1A antigen affinity purified polyclonal antibody (Catalog # PB9816) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAP1A at approximately 21KD. The expected band size for RAP1A is at 21KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rrm2-picoband-trade-antibody-pb9817-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9817-2-IHC-anti-rrm2-picoband-antibody.jpgAnti-RRM2 Antibody Picoband&reg; IHC analysis of RRM2 using anti-RRM2 antibody (PB9817). <br> RRM2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RRM2 Antibody (PB9817) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9817-rrm2-primary-antibodies-wb-testing-1.jpgAnti-RRM2 Antibody Picoband&reg;Western blot analysis of RRM2 using anti-RRM2 antibody (PB9817). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human SW620 whole cell lysates,<br> Lane 5: mouse HEPA1-6 whole cell lysates,<br> Lane 6: mouse Neuro-2a whole cell lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRM2 antigen affinity purified polyclonal antibody (PB9817) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RRM2 at approximately 45 kDa. The expected band size for RRM2 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-s100a11-picoband-trade-antibody-pb9818-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9818-1-WB-anti-s100a11-picoband-antibody.jpgAnti-S100A11 Antibody Picoband&reg; Western blot analysis of S100A11 using anti-S100A11 antibody (PB9818). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A11 antigen affinity purified polyclonal antibody (Catalog # PB9818) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A11 at approximately 15 kDa. The expected band size for S100A11 is at 15 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-munc18-1-picoband-trade-antibody-pb9819-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9819-stxbp1-primary-antibodies-wb-testing-1.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg; Western blot analysis of STXBP1 using anti-STXBP1 antibody (PB9819). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: rat C6 whole cell lysates, <br> Lane 4: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP1 antigen affinity purified polyclonal antibody (Catalog # PB9819) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STXBP1 at approximately 70 kDa. The expected band size for STXBP1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9819-stxbp1-primary-antibodies-ihc-testing-5.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg;IHC analysis of STXBP1 using anti-STXBP1 antibody (PB9819). <br>STXBP1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STXBP1 Antibody (PB9819) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9819-2-IHC-anti-munc18-1-picoband-antibody.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg; IHC analysis of Munc18-1 using anti-Munc18-1 antibody (PB9819). <br> Munc18-1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Munc18-1 Antibody (PB9819) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9819-3-IHC-anti-munc18-1-picoband-antibody.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg; IHC analysis of Munc18-1 using anti-Munc18-1 antibody (PB9819). <br> Munc18-1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Munc18-1 Antibody (PB9819) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9819-4-IHC-anti-munc18-1-picoband-antibody.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg; IHC analysis of Munc18-1 using anti-Munc18-1 antibody (PB9819). <br> Munc18-1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Munc18-1 Antibody (PB9819) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9819-stxbp1-primary-antibodies-if-testing-5.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg; IF analysis of STXBP1 using anti-STXBP1 antibody (PB9819). <br> STXBP1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-STXBP1 Antibody (PB9819) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9819-stxbp1-primary-antibodies-ip-testing-1.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg;Immunoprecipitating (IP) Munc18-1/STXBP1 in SH-SY5Y whole cell lysate.<br> Western blot analysis of Munc18-1/STXBP1 using anti-Munc18-1/STXBP1 antibody (PB9819); <br> Lane 1: SH-SY5Y whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Munc18-1/STXBP1 antibody in SH-SY5Y whole cell lysate;<br> Lane 3: anti-Munc18-1/STXBP1 antibody (2μg) + SH-SY5Y whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Munc18-1/STXBP1 antigen affinity purified polyclonal antibody (PB9819) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Munc18-1/STXBP1 at approximately 70 kDa. The expected band size for Munc18-1/STXBP1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9819-stxbp1-primary-antibodies-fcm-testing-6.jpgAnti-Munc18-1/STXBP1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-STXBP1 antibody (PB9819). <br>Overlay histogram showing A549 cells stained with PB9819 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STXBP1 Antibody (PB9819, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stxbp2-picoband-trade-antibody-pb9820-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9820-stxbp2-primary-antibodies-wb-testing-1.jpgAnti-STXBP2 Antibody Picoband&reg; Western blot analysis of STXBP2 using anti-STXBP2 antibody (PB9820). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STXBP2 antigen affinity purified polyclonal antibody (Catalog # PB9820) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STXBP2 at approximately 66 kDa. The expected band size for STXBP2 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9820-stxbp2-primary-antibodies-if-testing-2.jpgAnti-STXBP2 Antibody Picoband&reg; IF analysis of STXBP2 using anti-STXBP2 antibody (PB9820). <br> STXBP2 was detected in immunocytochemical section of SK-OV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-STXBP2 Antibody (PB9820) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9820-stxbp2-primary-antibodies-fcm-testing-3.jpgAnti-STXBP2 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-STXBP2 antibody (PB9820).<br>Overlay histogram showing U937 cells stained with PB9820 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STXBP2 Antibody (PB9820,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-prolactin-antibody-rp1021-boster.html2026-03-24T05:05:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1021-1-WB-anti-prolactin-antibody.jpgAnti-Prolactin Prl Antibody Picoband&reg;Western blot analysis of Prolactin expression in mouse testis extract (lane 1). Prolactin at 26KD was detected using rabbit anti-Prolactin Antigen Affinity purified polyclonal antibody (Catalog # RP1021) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sult2a1-picoband-trade-antibody-pb9821-boster.html2026-03-25T05:22:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9821-sult2a1-primary-antibodies-wb-testing-1.jpgAnti-SULT2A1 Antibody Picoband&reg; Western blot analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: rat liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2A1 antigen affinity purified polyclonal antibody (Catalog # PB9821) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2A1 at approximately 30-35 kDa. The expected band size for SULT2A1 is at 34 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9821-2-IHC-anti-sult2a1-picoband-antibody.jpgAnti-SULT2A1 Antibody Picoband&reg; IHC analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). <br> SULT2A1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2A1 Antibody (PB9821) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9821-3-IHC-anti-sult2a1-picoband-antibody.jpgAnti-SULT2A1 Antibody Picoband&reg; IHC analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). <br> SULT2A1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2A1 Antibody (PB9821) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9821-4-IHC-anti-sult2a1-picoband-antibody.jpgAnti-SULT2A1 Antibody Picoband&reg; IHC analysis of SULT2A1 using anti-SULT2A1 antibody (PB9821). <br> SULT2A1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2A1 Antibody (PB9821) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tap1-picoband-trade-antibody-pb9823-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9823-1-WB-anti-tap1-picoband-antibody.jpgAnti-TAP1 Antibody Picoband&reg; Western blot analysis of TAP1 using anti-TAP1 antibody (PB9823). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: HUT Whole Cell Lysate,<br> Lane 3: SW620 Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP1 antigen affinity purified polyclonal antibody (Catalog # PB9823) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAP1 at approximately 87 kDa. The expected band size for TAP1 is at 87 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9823-2-IHC-anti-tap1-picoband-antibody.jpgAnti-TAP1 Antibody Picoband&reg; IHC analysis of TAP1 using anti-TAP1 antibody (PB9823). <br> TAP1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TAP1 Antibody (PB9823) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tap2-picoband-trade-antibody-pb9824-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9824-1-WB-anti-tap2-picoband-antibody.jpgAnti-TAP2 Antibody Picoband&reg; Western blot analysis of TAP2 using anti-TAP2 antibody (PB9824). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAP2 antigen affinity purified polyclonal antibody (Catalog # PB9824) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAP2 at approximately 87 kDa. The expected band size for TAP2 is at 87 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tcp1-picoband-trade-antibody-pb9826-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9826-tcp1-alpha-primary-antibodies-wb-testing-1.jpgAnti-TCP1 alpha Antibody Picoband&reg; Western blot analysis of TCP1 using anti-TCP1 antibody (PB9826). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human MOLT4 whole cell lysates,<br> Lane 5: human Jurkat whole cell lysates,<br> Lane 6: human A549 whole cell lysates,<br> Lane 7: human MCF-7 whole cell lysates,<br> Lane 8: human U251 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCP1 antigen affinity purified polyclonal antibody (Catalog # PB9826) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCP1 at approximately 60 kDa. The expected band size for TCP1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9826-tcp1-alpha-primary-antibodies-wb-testing-2.jpgAnti-TCP1 alpha Antibody Picoband&reg; Western blot analysis of TCP1 using anti-TCP1 antibody (PB9826). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat heart tissue lysates,<br> Lane 2: rat ovary tissue lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: rat lung tissue lysates,<br> Lane 5: mouse heart tissue lysates,<br> Lane 6: mouse ovary tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCP1 antigen affinity purified polyclonal antibody (Catalog # PB9826) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCP1 at approximately 60 kDa. The expected band size for TCP1 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9826-tcp1-alpha-primary-antibodies-ihc-testing-3.jpgAnti-TCP1 alpha Antibody Picoband&reg; IHC analysis of TCP1 using anti-TCP1 antibody (PB9826). <br> TCP1 was detected in a paraffin-embedded section of human ovary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCP1 Antibody (PB9826) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9826-tcp1-alpha-primary-antibodies-ihc-testing-4.jpgAnti-TCP1 alpha Antibody Picoband&reg; IHC analysis of TCP1 using anti-TCP1 antibody (PB9826). <br> TCP1 was detected in a paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCP1 Antibody (PB9826) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9826-tcp1-alpha-primary-antibodies-ihc-testing-5.jpgAnti-TCP1 alpha Antibody Picoband&reg; IHC analysis of TCP1 using anti-TCP1 antibody (PB9826). <br> TCP1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCP1 Antibody (PB9826) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9826-tcp1-alpha-primary-antibodies-ihc-testing-6.jpgAnti-TCP1 alpha Antibody Picoband&reg; IHC analysis of TCP1 using anti-TCP1 antibody (PB9826). <br> TCP1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TCP1 Antibody (PB9826) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9826-tcp1-alpha-primary-antibodies-if-testing-7.jpgAnti-TCP1 alpha Antibody Picoband&reg; IF analysis of TCP1 using anti- TCP1 antibody (PB9077). <br> TCP1 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- TCP1 Antibody (PB9077) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-transferrin-picoband-trade-antibody-pb9827-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9827-41598_2021_91521_fig2_html.pngAnti-Transferrin/TF Antibody Picoband&reg;Comparison of the binding ability of PVDF membrane and NC membrane to medium molecular weight proteins. ( a ) The pooled sera proteins (0.1–3.0 μg) were subjected to 8% SDS-PAGE. The electroblotted membranes are PVDF membrane (up) and NC membrane (down), respectively. The membranes were incubated with anti-alpha-1-acid glycoprotein (AGP), anti-eukaryotic transformation extension factor 1 alpha 2 (EEF1A2) and anti-transferrin (TF) antibodies. ( b ) Staining intensities were statistically analyzed (n = 3 individual experiments). Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. N.S., not significant. All values are means ± S.E. (error bars). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-91521-8'>34103620</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9827-tf-primary-antibodies-wb-testing-1.jpgAnti-Transferrin/TF Antibody Picoband&reg; Western blot analysis of Transferrin using anti-Transferrin antibody (PB9827). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse liver tissue lysates,<br> Lane 7: mouse Hepa1/6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Transferrin antigen affinity purified polyclonal antibody (Catalog # PB9827) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Transferrin at approximately 77 kDa. The expected band size for Transferrin is at 77 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9827-tf-primary-antibodies-ihc-testing-2.jpgAnti-Transferrin/TF Antibody Picoband&reg; IHC analysis of Transferrin using anti-Transferrin antibody (PB9827). <br> Transferrin was detected in a paraffin-embedded section of human esophageal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Transferrin Antibody (PB9827) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9827-tf-primary-antibodies-fcm-testing-3.jpgAnti-Transferrin/TF Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-Transferrin antibody (PB9827). <br> Overlay histogram showing HepG2 cells stained with PB9827 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Transferrin Antibody (PB9827, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tgfbr1-picoband-trade-antibody-pb9828-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9828-1_1.jpgAnti-TGF beta Receptor I/TGFBR1 Antibody Picoband&reg; Western blot analysis of TGFBR1/Tgf Beta Receptor I using anti-TGFBR1/Tgf Beta Receptor I antibody (PB9828). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates&#44; <br> Lane 2: human SW579 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFBR1/Tgf Beta Receptor I antigen affinity purified polyclonal antibody (Catalog # PB9828) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFBR1/Tgf Beta Receptor I at approximately 50-56KD. The expected band size for TGFBR1/Tgf Beta Receptor I is at 56KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tgfbr2-picoband-trade-antibody-pb9829-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9829-tgfbr2-primary-antibodies-wb-testing-1.jpgAnti-TGF beta Receptor II/TGFBR2 Antibody Picoband&reg; Western blot analysis of TGFBR2 using anti-TGFBR2 antibody (PB9829). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFBR2 antigen affinity purified polyclonal antibody (Catalog # PB9829) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFBR2 at approximately 70 kDa. The expected band size for TGFBR2 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp2-picoband-trade-antibody-pb9830-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9830-1-WB-anti-timp-2-antibody.jpgAnti-TIMP2 Antibody Picoband&reg; Western blot analysis of TIMP2 using anti-TIMP2 antibody (PB9830). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Spleen Tissue Lysate at 50ug,<br> Lane 2: Rat Testis Tissue Lysate at 50ug,<br> Lane 3: Mouse Brain Tissue Lysate at 50ug,<br> Lane 4: Mouse Thymus Tissue Lysate at 50ug,<br> Lane 5: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP2 antigen affinity purified polyclonal antibody (Catalog # PB9830) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP2 at approximately 24 kDa. The expected band size for TIMP2 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9830-ijo-16-09-1441-g004.jpgAnti-TIMP2 Antibody Picoband&reg;17β-estradiol inhibits the production of TIMPs and MMPs in HTFs. Representative graphs showing the production of MMP-1, MMP-3, proMMP-2, active MMP-2, TIMP-1, and TIMP-2 in the supernatants of HTFs after the treatment of indicated concentration of 17β-estradiol based on immunoblot and gelatin zymography. MMP-1 and MMP-3 in the supernatants but not proMMP-2 and active MMP-2 were decreased following the pre-incubation with 17β-estradiol. In addition, 17β-estradiol induced a concentration-dependent reduction in the level of TIMP-2 but not TIMP-1 in the supernatants of HTFs exposed to TGF-β (5 ng/mL). HTF: Human Tenon fibroblasts; TGF: Transforming growth factor; MMP: Matrix metalloproteinases; TIMPs: Tissue inhibitors of metalloproteinases.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10475634/&orig_host=www.ncbi.nlm.nih.gov'>37724268</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp3-picoband-trade-antibody-pb9831-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9831-1-WB-anti-timp-3-antibody.jpgAnti-TIMP3 Antibody Picoband&reg; Western blot analysis of TIMP3 using anti-TIMP3 antibody (PB9831). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Kidney Tissue Lysate at 50ug,<br> Lane 2: Mouse Ovary Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: MCF-7 Whole Cell Lysate at 40ug,<br> Lane 5: SMMC Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP3 antigen affinity purified polyclonal antibody (Catalog # PB9831) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP3 at approximately 34 kDa. The expected band size for TIMP3 is at 34 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tk1-picoband-trade-antibody-pb9832-boster.html2026-03-24T05:05:23+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9832-1-WB-anti-tk1-thymidine-kinase-picoband-antibody.jpgAnti-Thymidine Kinase 1/TK1 Antibody Picoband&reg; Western blot analysis of TK1 using anti-TK1 antibody (PB9832). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.<br> Lane 1: HELA Whole Cell Lysate,<br> Lane 2: SMMC Whole Cell Lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TK1 antigen affinity purified polyclonal antibody (Catalog # PB9832) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TK1 at approximately 25 kDa. The expected band size for TK1 is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnxb-picoband-trade-antibody-pb9833-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9833-2-IHC-anti-tnxb-tenascin-x-picoband-antibody.jpgAnti-TNXB Antibody Picoband&reg; IHC analysis of TNXB using anti-TNXB antibody (PB9833). <br> TNXB was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TNXB Antibody (PB9833) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9833-tnxb-primary-antibodies-wb-testing-1.jpgAnti-TNXB Antibody Picoband&reg;Western blot analysis of TNXB using anti-TNXB antibody (PB9833). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNXB antigen affinity purified polyclonal antibody (PB9833) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TNXB at approximately 458 kDa. The expected band size for TNXB is at 458 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9833_13023_2025_3858_fig3_html.pngAnti-TNXB Antibody Picoband&reg;IHC results in patients 1, 2, 7 and Immunofluorescence staining Patient 4. Immunohistochemistry results in patient 7 ( B ) showed collagen expression was widely reduced compared with controls ( A ) in basement membrane. The expression of type III collagen from patients 1( D ) and 2( E ) significantly lower compared with controls ( C ). There are large quantities of type I collagen, uniformly distributed in normal fibroblasts ( F ). In contrast, COL1A1 expression was reduced and significantly unevenly distributed in the fibroblasts of Patient 4(G). (( A ) and ( B ), TNXB antibody IHC stain ×40; ( C ), ( D ) and ( E ), COL3A1 antibody IHC stain ×40; ( F ) and ( G ), immunofluorescence stain ×20) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13023-025-03858-2'>40598578</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9833-tnxb-primary-antibodies-if-testing-3.jpgAnti-TNXB Antibody Picoband&reg; IF analysis of TNXB using anti-TNXB antibody (PB9833). <br> TNXB was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TNXB Antibody (PB9833) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9833-tnxb-primary-antibodies-fcm-testing-4.jpgAnti-TNXB Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-TNXB antibody (PB9833).<br>Overlay histogram showing Hela cells stained with PB9833 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TNXB Antibody (PB9833,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpm8-picoband-trade-antibody-pb9837-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9837-1-WB-anti-trpm8-picoband-antibody.jpgAnti-TRPM8 Antibody Picoband&reg; Western blot analysis of TRPM8 using anti-TRPM8 antibody (PB9837). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: 22RV1 whole cell lysates,<br> Lane 3: SW620 whole cell lysates,<br> Lane 4: A549 whole cell lysates,<br> Lane 5: A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM8 antigen affinity purified polyclonal antibody (Catalog # PB9837) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM8 at approximately 127 kDa. The expected band size for TRPM8 is at 127 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ube1c-picoband-trade-antibody-pb9838-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9838-ube1c-primary-antibodies-wb-testing-1.jpgAnti-UBE1C/UBA3 Antibody Picoband&reg; Western blot analysis of UBE1C using anti-UBE1C antibody (PB9838). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysate,<br> Lane 2: human HEK293 whole cell lysate,<br> Lane 3: rat brain tissue lysate.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE1C antigen affinity purified polyclonal antibody (Catalog # PB9838) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE1C at approximately 52 kDa. The expected band size for UBE1C is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9838-2-IHC-anti-ube1c-picoband-antibody.jpgAnti-UBE1C/UBA3 Antibody Picoband&reg; IHC analysis of UBE1C using anti-UBE1C antibody (PB9838). <br> UBE1C was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UBE1C Antibody (PB9838) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9838-3-IHC-anti-ube1c-picoband-antibody.jpgAnti-UBE1C/UBA3 Antibody Picoband&reg; IHC analysis of UBE1C using anti-UBE1C antibody (PB9838). <br> UBE1C was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UBE1C Antibody (PB9838) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9838-4.jpgAnti-UBE1C/UBA3 Antibody Picoband&reg; IF analysis of UBE1C using anti-UBE1C antibody (PB9838). <br> UBE1C was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-UBE1C Antibody (PB9838) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9838-5.jpgAnti-UBE1C/UBA3 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-UBE1C antibody (PB9838).<br>Overlay histogram showing A549 cells stained with PB9838 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE1C Antibody (PB9838,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ube2q2-picoband-trade-antibody-pb9839-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9839-ube2q2-primary-antibodies-wb-testing-1.jpgAnti-UBE2Q2 Antibody Picoband&reg;Western blot analysis of UBE2Q2 using anti-UBE2Q2 antibody (PB9839). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human U87 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: rat stomach tissue lysates,<br> Lane 6: rat pancrease tissue lysates,<br> Lane 7: mouse pancrease tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2Q2 antigen affinity purified polyclonal antibody (PB9839) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UBE2Q2 at approximately 49 kDa. The expected band size for UBE2Q2 is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9839-ube2q2-primary-antibodiesfcm-testing-1.jpgAnti-UBE2Q2 Antibody Picoband&reg;Flow Cytometry analysis of HepG2 cells using anti-UBE2Q2 antibody (PB9839). <br> Overlay histogram showing HepG2 cells stained with PB9839 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2Q2 Antibody (PB9839, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-unc5c-picoband-trade-antibody-pb9841-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9841-1-WB-anti-unc5c-unc5h3-picoband-antibody.jpgAnti-UNC5C Antibody Picoband&reg; Western blot analysis of UNC5C using anti-UNC5C antibody (PB9841). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Brain Tissue Lysate at 50ug,<br> Lane 2: Mouse Brain Tissue Lysate at 50ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UNC5C antigen affinity purified polyclonal antibody (Catalog # PB9841) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UNC5C at approximately 115 kDa. The expected band size for UNC5C is at 115 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9841-unc5c-primary-antibodies-fcm-testing-2.jpgAnti-UNC5C Antibody Picoband&reg; Flow Cytometry analysis of SH-SY5Y cells using anti-UNC5C antibody (PB9841). <br> Overlay histogram showing SH-SY5Y cells stained with PB9841 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-UNC5C Antibody (PB9841, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rent1-hupf1-picoband-trade-antibody-pb9842-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9842-upf1-primary-antibodies-wb-testing-1.jpgAnti-RENT1/hUPF1 Antibody Picoband&reg; Western blot analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (PB9842). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Raji whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human SK-OV-3 whole cell lysates,<br> Lane 5: human PC-3 whole cell lysates,<br> Lane 6: human HEK293 whole cell lysates,<br> Lane 7: rat RH35 whole cell lysates,<br> Lane 8: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RENT1/hUPF1 antigen affinity purified polyclonal antibody (Catalog # PB9842) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RENT1/hUPF1 at approximately 140 kDa. The expected band size for RENT1/hUPF1 is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9842-2-IHC-anti-rent1-hupf1-picoband-antibody.jpgAnti-RENT1/hUPF1 Antibody Picoband&reg; IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (PB9842). <br> RENT1/hUPF1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RENT1/hUPF1 Antibody (PB9842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9842-3-IHC-anti-rent1-hupf1-picoband-antibody.jpgAnti-RENT1/hUPF1 Antibody Picoband&reg; IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (PB9842). <br> RENT1/hUPF1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RENT1/hUPF1 Antibody (PB9842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9842-4-IHC-anti-rent1-hupf1-picoband-antibody.jpgAnti-RENT1/hUPF1 Antibody Picoband&reg; IHC analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (PB9842). <br> RENT1/hUPF1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-RENT1/hUPF1 Antibody (PB9842) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9842-5.pngAnti-RENT1/hUPF1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-RENT1/hUPF1 antibody (PB9842). <br> Overlay histogram showing PC-3 cells stained with PB9842 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RENT1/hUPF1 Antibody (PB9842&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9842-6.jpgAnti-RENT1/hUPF1 Antibody Picoband&reg; IF analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (PB9842).<br> RENT1/hUPF1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RENT1/hUPF1 Antibody (PB9842) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-upf3b-rent3b-picoband-trade-antibody-pb9843-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9843-upf3b-primary-antibodies-wb-testing-1.jpgAnti-UPF3B/RENT3B Antibody Picoband&reg; Western blot analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.<br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human 22RV1 whole cell lysates, <br> Lane 3: human U87 whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates,<br> Lane 5: rat brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPF3B/RENT3B antigen affinity purified polyclonal antibody (Catalog # PB9843) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPF3B/RENT3B at approximately 58 kDa. The expected band size for UPF3B/RENT3B is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9843-fendo-15-1359147-g003.jpgAnti-UPF3B/RENT3B Antibody Picoband&reg;Cytokine-induced suppression of NMD activity is associated with UPF3B downregulation and attenuated by UPF3 overexpression in β cells. EndoC-βH3 cells were cotransfected with empty vector (E.V.), UPF3A, UPF3B, and/or UPF3BΔ42 (dominant negative of UPF3B) plasmids, and then with Renilla -HBB(PTC − ) and or Renilla -HBB(PTC + ), along with the Firefly plasmid and exposed to cytokine combination (Cyt; 3 ng/mL IL-1β + 10 ng/mL IFN-γ + 10 ng/mL TNF-α) for 18 (h) (A (i, ii) ) mRNA level of Upf3A and Upf3B genes in EndoC-βH3 cells was quantified by RT-qPCR and normalised to tubulin mRNAs. (B) Luciferase activity was measured in the lysate of the transfected cells and represented as NMD activity calculated by dividing luciferase activity of HBB(PTC − )/HBB(PTC + ) as explained in the Methods. The overexpression of UPF3A and UPF3B proteins was examined by Western blot analysis [ Supplementary Figure 4B (i)]. The data are means ± SEM of N = 6. The symbol “ * ” indicates the Bonferroni-corrected paired t -test values of treated versus untreated E.V. (Unt) (A, B) or, otherwise, cytokine (Cyt)-treated E.V. that is designated by a line on top of the bars (B) : * ≤ 0.05; ** ≤ 0.01. ns, nonsignificant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2024.1359147/full'>38586449</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9843-fendo-15-1359147-g004.jpgAnti-UPF3B/RENT3B Antibody Picoband&reg;UPF3 overexpression deteriorates cell viability and reduces insulin content but not secretion in EndoC-βH3 cells. EndoC-βH3 cells were cotransfected with empty vector (E.V.), UPF3A, and/or UPF3B plasmids and exposed to cytokine combination (Cyt; 3 ng/mL IL-1β + 10 ng/mL IFN-γ + 10 ng/mL TNF-α) for 3 days. (A) Cell viability was measured by Alamarblue (i) and caspase-3 activity (ii) assays ( N = 6). (B) Glucose-stimulated insulin secretion (GSIS) (i) and insulin contents (iii) were investigated in the transfected EndoC-βH3 cells. Insulin concentration (ng/mL) was measured by insulin ultrasensitive assay ( N = 6). GSIS index (ii) was calculated by dividing the insulin concentration measured in the treatments at 17 mM by 2 mM of glucose. The data are means ± SEM of N = 6. The symbol “ * ” indicates the Bonferroni-corrected paired t -test values of treated versus untreated E.V. (Unt) or, otherwise, cytokine (Cyt)-treated E.V. that is designated by a line on top of the bars (A) or the Bonferroni-corrected paired t -test values of the corresponding low versus high glucose, that is otherwise designated by lines on top of the bars (B (i) ) : * ≤ 0.05; ** ≤ 0.01; **** ≤ 0.0001. ns, nonsignificant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/endocrinology/articles/10.3389/fendo.2024.1359147/full'>38586449</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9843-2-IHC-anti-upf3b-rent3b-picoband-antibody.jpgAnti-UPF3B/RENT3B Antibody Picoband&reg; IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843). <br> UPF3B/RENT3B was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9843-3-IHC-anti-upf3b-rent3b-picoband-antibody.jpgAnti-UPF3B/RENT3B Antibody Picoband&reg; IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843). <br> UPF3B/RENT3B was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9843-4-IHC-anti-upf3b-rent3b-picoband-antibody.jpgAnti-UPF3B/RENT3B Antibody Picoband&reg; IHC analysis of UPF3B/RENT3B using anti-UPF3B/RENT3B antibody (PB9843). <br> UPF3B/RENT3B was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UPF3B/RENT3B Antibody (PB9843) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trif-antibody-rp1100-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1100-ticam1-primary-antibodies-wb-testing-1.jpgAnti-TRIF/TICAM1 Antibody Picoband&reg;Western blot analysis of TRIF/TICAM1 using anti-TRIF/TICAM1 antibody (RP1100). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates,<br> Lane 2: mouse testis tissue lysates,<br> Lane 3: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIF/TICAM1 antigen affinity purified polyclonal antibody (RP1100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TRIF/TICAM1 at approximately 110 kDa. The expected band size for TRIF/TICAM1 is at 79 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sult2b1-picoband-trade-antibody-pb9822-boster.html2026-03-24T05:05:24+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9822-1-WB-anti-sult2b1-picoband-antibody.jpgAnti-SULT2B1 Antibody Picoband&reg; Western blot analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: Rat Testis Tissue Lysate at 50ug,<br> Lane 2: A431 Whole Cell Lysate at 40ug,<br> Lane 3: HELA Whole Cell Lysate at 40ug,<br> Lane 4: MCF-7 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody (Catalog # PB9822) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9822-2-IHC-anti-sult2b1-picoband-antibody.jpgAnti-SULT2B1 Antibody Picoband&reg; IHC analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). <br> SULT2B1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2B1 Antibody (PB9822) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9822-3-IHC-anti-sult2b1-picoband-antibody.jpgAnti-SULT2B1 Antibody Picoband&reg; IHC analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). <br> SULT2B1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SULT2B1 Antibody (PB9822) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9822-sult2b1-primary-antibodies-if-testing-4.jpgAnti-SULT2B1 Antibody Picoband&reg; IF analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). <br> SULT2B1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SULT2B1 Antibody (PB9822) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9822-sult2b1-primary-antibodies-fcm-testing-5.jpgAnti-SULT2B1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-SULT2B1 antibody (PB9822).<br>Overlay histogram showing A549 cells stained with PB9822 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SULT2B1 Antibody (PB9822,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trap1-picoband-trade-antibody-pb9834-boster.html2026-03-24T05:05:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9834-1-WB-anti-trap1-hsp-75-picoband-antibody.jpgAnti-TRAP1 Antibody Picoband&reg; Western blot analysis of TRAP1 using anti-TRAP1 antibody (PB9834). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.<br> Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug,<br> Lane 2: Rat Kidney Tissue Lysate at 50ug,<br> Lane 3: Rat Brain Tissue Lysate at 50ug,<br> Lane 4: SMMC Whole Cell Lysate at 40ug,<br> Lane 5: PANC Whole Cell Lysate at 40ug,<br> Lane 6: A549 Whole Cell Lysate at 40ug.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAP1 antigen affinity purified polyclonal antibody (Catalog # PB9834) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAP1 at approximately 80 kDa. The expected band size for TRAP1 is at 80 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9834-2-IHC-anti-trap1-hsp-75-picoband-antibody.jpgAnti-TRAP1 Antibody Picoband&reg; IHC analysis of TRAP1 using anti-TRAP1 antibody (PB9834). <br> TRAP1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRAP1 Antibody (PB9834) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kap1-picoband-trade-antibody-pb9835-boster.html2026-03-24T05:05:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9835-kap1-primary-antibodies-wb-testing-1.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; Western blot analysis of KAP1 using anti-KAP1 antibody (PB9835). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human PC-3 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse spleen tissue lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAP1 antigen affinity purified polyclonal antibody (Catalog # PB9835) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAP1 at approximately 100 kDa. The expected band size for KAP1 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9835-2-IHC-anti-kap1-picoband-antibody.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IHC analysis of KAP1 using anti-KAP1 antibody (PB9835). <br> KAP1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9835-3-IHC-anti-kap1-picoband-antibody.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IHC analysis of KAP1 using anti-KAP1 antibody (PB9835). <br> KAP1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9835-4-IHC-anti-kap1-picoband-antibody.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IHC analysis of KAP1 using anti-KAP1 antibody (PB9835). <br> KAP1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9835-kap1-primary-antibodies-ihc-testing-5.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IHC analysis of KAP1 using anti-KAP1 antibody (PB9835).<br> KAP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9835-kap1-primary-antibodies-ihc-testing-6.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IHC analysis of KAP1 using anti-KAP1 antibody (PB9835).<br> KAP1 was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9835-kap1-primary-antibodies-ihc-testing-7.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IHC analysis of KAP1 using anti-KAP1 antibody (PB9835).<br> KAP1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9835-kap1-primary-antibodies-ihc-testing-8.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IHC analysis of KAP1 using anti-KAP1 antibody (PB9835).<br> KAP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9835-kap1-primary-antibodies-if-testing-9.jpgAnti-KAP1/TRIM28 Antibody Picoband&reg; IF analysis of KAP1 using anti-KAP1 antibody (PB9835). <br> KAP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-KAP1 Antibody (PB9835) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9835-10.pngAnti-KAP1/TRIM28 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-KAP1 antibody (PB9835). <br> Overlay histogram showing U20S cells stained with PB9835 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KAP1 Antibody (PB9835&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tif1-gamma-picoband-trade-antibody-pb9836-boster.html2026-03-24T05:05:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9836-trim33-primary-antibodies-wb-testing-1.jpgAnti-TIF1 gamma/TRIM33 Antibody Picoband&reg; Western blot analysis of TIF1 gamma using anti-TIF1 gamma antibody (PB9836). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIF1 gamma antigen affinity purified polyclonal antibody (Catalog # PB9836) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIF1 gamma at approximately 150 kDa. The expected band size for TIF1 gamma is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9836-trim33-primary-antibodies-ihc-testing-2.jpgAnti-TIF1 gamma/TRIM33 Antibody Picoband&reg; IHC analysis of TIF1 gamma using anti-TIF1 gamma antibody (PB9836). <br> TIF1 gamma was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIF1 gamma Antibody (PB9836) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9836-trim33-primary-antibodies-ihc-testing-3.jpgAnti-TIF1 gamma/TRIM33 Antibody Picoband&reg; IHC analysis of TIF1 gamma using anti-TIF1 gamma antibody (PB9836). <br> TIF1 gamma was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TIF1 gamma Antibody (PB9836) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9836-5.pngAnti-TIF1 gamma/TRIM33 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-TIF1 gamma antibody (PB9836). <br> Overlay histogram showing U87 cells stained with PB9836 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIF1 gamma Antibody (PB9836&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9836-4.jpgAnti-TIF1 gamma/TRIM33 Antibody Picoband&reg; IF analysis of TIF1 gamma using anti-TIF1 gamma antibody (PB9836). <br> TIF1 gamma was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-TIF1 gamma Antibody (PB9836) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9836-6.pngAnti-TIF1 gamma/TRIM33 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-TIF1 gamma antibody (PB9836). <br> Overlay histogram showing A431 cells stained with PB9836 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIF1 gamma Antibody (PB9836&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pgp9-5-picoband-trade-antibody-pb9840-boster.html2026-03-24T05:05:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9840-pgp9.5-primary-antibodies-wb-testing-1.jpgAnti-PGP9.5/UCHL1 Antibody Picoband&reg; Western blot analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates, <br> Lane 2: human SH-SY5Y whole cell lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: rat C6 whole cell lysates, <br> Lane 3: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP9.5 antigen affinity purified polyclonal antibody (Catalog # PB9840) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGP9.5 at approximately 25 kDa. The expected band size for PGP9.5 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9840-2-IHC-anti-pgp9-5-picoband-antibody.jpgAnti-PGP9.5/UCHL1 Antibody Picoband&reg; IHC analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). <br> PGP9.5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PGP9.5 Antibody (PB9840) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9840-3-IHC-anti-pgp9-5-picoband-antibody.jpgAnti-PGP9.5/UCHL1 Antibody Picoband&reg; IHC analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). <br> PGP9.5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PGP9.5 Antibody (PB9840) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9840-4-IHC-anti-pgp9-5-picoband-antibody.jpgAnti-PGP9.5/UCHL1 Antibody Picoband&reg; IHC analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). <br> PGP9.5 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PGP9.5 Antibody (PB9840) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9840-5.jpgAnti-PGP9.5/UCHL1 Antibody Picoband&reg; IF analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). <br> PGP9.5 was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-PGP9.5 Antibody (PB9840) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9840-6.jpgAnti-PGP9.5/UCHL1 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-PGP9.5 antibody (PB9840).<br>Overlay histogram showing K562 cells stained with PB9840 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGP9.5 Antibody (PB9840,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-galectin-1-picoband-trade-antibody-pb9240-1-boster.html2026-03-24T05:05:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9240-1-lgals1-primary-antibodies-wb-testing-1.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; Western blot analysis of Galectin 1 using anti-Galectin 1 antibody (PB9240-1). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human A375 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat kidney tissue lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse kidney tissue lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin 1 antigen affinity purified polyclonal antibody (Catalog # PB9240-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galectin 1 at approximately 15 kDa. The expected band size for Galectin 1 is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9240-1-2-IHC-anti-galectin-1-antibody.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of Galectin 1 using anti-Galectin 1 antibody (PB9240-1). <br> Galectin 1 was detected in a paraffin-embedded section of mouse intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Galectin 1 Antibody (PB9240-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9240-1-3-IHC-anti-galectin-1-antibody.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of Galectin 1 using anti-Galectin 1 antibody (PB9240-1). <br> Galectin 1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Galectin 1 Antibody (PB9240-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9240-1-4-IHC-anti-galectin-1-antibody.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IHC analysis of Galectin 1 using anti-Galectin 1 antibody (PB9240-1). <br> Galectin 1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Galectin 1 Antibody (PB9240-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9240-1-lgals1-primary-antibodies-if-testing-5.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; IF analysis of Galectin 1 using anti-Galectin 1 antibody (PB9240-1). <br> Galectin 1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Galectin 1 Antibody (PB9240-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9240-1-6.jpgAnti-Galectin 1/LGALS1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-Galectin 1 antibody (PB9240-1). <br>Overlay histogram showing PC-3 cells stained with PB9240-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Galectin 1 Antibody (PB9240-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apoa1-picoband-trade-antibody-pb9844-boster.html2026-03-24T05:05:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9844-apoa1-primary-antibodies-wb-testing-1.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; Western blot analysis of APOA1 using anti-APOA1 antibody (PB9844). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (PB9844) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 25 kDa. The expected band size for APOA1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9844-2-IHC-anti-apoa1-apoa-i-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; IHC analysis of APOA1 using anti-APOA1 antibody (PB9844). <br> APOA1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOA1 Antibody (PB9844) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9844-3-IHC-anti-apoa1-apoa-i-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; IHC analysis of APOA1 using anti-APOA1 antibody (PB9844). <br> APOA1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOA1 Antibody (PB9844) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apoa1-picoband-trade-antibody-pb9845-boster.html2026-03-24T05:05:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9845-1-WB-anti-apoa1-apoa-i-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; Western blot analysis of APOA1 using anti-APOA1 antibody (PB9845). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: rat testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9845) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 24 kDa. The expected band size for APOA1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9845-2-IHC-anti-apoa1-apoa-i-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; IHC analysis of APOA1 using anti-APOA1 antibody (PB9845). <br> APOA1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOA1 Antibody (PB9845) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9845-3-IHC-anti-apoa1-apoa-i-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; IHC analysis of APOA1 using anti-APOA1 antibody (PB9845). <br> APOA1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOA1 Antibody (PB9845) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcar3-picoband-trade-antibody-pb9846-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9846-1-WB-anti-bcar3-picoband-antibody.jpgAnti-BCAR3 Antibody Picoband&reg; Western blot analysis of BCAR3 using anti-BCAR3 antibody (PB9846). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HEPG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAR3 antigen affinity purified polyclonal antibody (Catalog # PB9846) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAR3 at approximately 93 kDa. The expected band size for BCAR3 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9846-2-IHC-anti-bcar3-picoband-antibody.jpgAnti-BCAR3 Antibody Picoband&reg; IHC analysis of BCAR3 using anti-BCAR3 antibody (PB9846). <br> BCAR3 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BCAR3 Antibody (PB9846) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9846-3-IHC-anti-bcar3-picoband-antibody.jpgAnti-BCAR3 Antibody Picoband&reg; IHC analysis of BCAR3 using anti-BCAR3 antibody (PB9846). <br> BCAR3 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BCAR3 Antibody (PB9846) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9846-4-IHC-anti-bcar3-picoband-antibody.jpgAnti-BCAR3 Antibody Picoband&reg; IHC analysis of BCAR3 using anti-BCAR3 antibody (PB9846). <br> BCAR3 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BCAR3 Antibody (PB9846) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd14-picoband-trade-antibody-pb9847-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9847-cd14-primary-antibodies-wb-testing-1.jpgAnti-CD14 Antibody Picoband&reg; Western blot analysis of CD14 using anti-CD14 antibody (PB9847). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD14 antigen affinity purified polyclonal antibody (Catalog # PB9847) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD14 at approximately 50-55 kDa. The expected band size for CD14 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9847-cd14-primary-antibodies-ihc-testing-2.jpgAnti-CD14 Antibody Picoband&reg; IHC analysis of CD14 using anti-CD14 antibody (PB9847). <br> CD14 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (PB9847) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9847-cd14-primary-antibodies-ihc-testing-3.jpgAnti-CD14 Antibody Picoband&reg; IHC analysis of CD14 using anti-CD14 antibody (PB9847). <br> CD14 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (PB9847) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9847-cd14-primary-antibodies-ihc-testing-4.jpgAnti-CD14 Antibody Picoband&reg; IHC analysis of CD14 using anti-CD14 antibody (PB9847). <br> CD14 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD14 Antibody (PB9847) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd46-picoband-trade-antibody-pb9848-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9848-1-WB-anti-cd46-picoband-antibody.jpgAnti-CD46 Antibody Picoband&reg; Western blot analysis of CD46 using anti-CD46 antibody (PB9848). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse testis tissue lysates,<br> Lane 2: NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD46 antigen affinity purified polyclonal antibody (Catalog # PB9848) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD46 at approximately 41 kDa, 70 kDa. The expected band size for CD46 is at 41 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdc25c-picoband-trade-antibody-pb9849-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9849-1-WB-anti-cdc25c-picoband-antibody.jpgAnti-Cdc25C Antibody Picoband&reg; Western blot analysis of Cdc25C using anti-Cdc25C antibody (PB9849). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: SW620 whole cell lysates,<br> Lane 3: MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdc25C antigen affinity purified polyclonal antibody (Catalog # PB9849) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdc25C at approximately 53 kDa. The expected band size for Cdc25C is at 53 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-factor-d-picoband-trade-antibody-pb9850-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9850-1-WB-anti-cfd-adipsin-antibody.jpgAnti-Factor D/CFD Antibody Picoband&reg; Western blot analysis of Factor D using anti-Factor D antibody (PB9850). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody (Catalog # PB9850) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor D at approximately 43 kDa. The expected band size for Factor D is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cntf-picoband-trade-antibody-pb9851-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9851-1-WB-anti-cntf-picoband-antibody.jpgAnti-CNTF Antibody Picoband&reg; Western blot analysis of CNTF using anti-CNTF antibody (PB9851). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNTF antigen affinity purified polyclonal antibody (Catalog # PB9851) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNTF at approximately 27 kDa. The expected band size for CNTF is at 23 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ceruloplasmin-picoband-trade-antibody-pb9852-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9852-ceruloplasmin-primary-antibodies-wb-testing-1.jpgAnti-Ceruloplasmin/CP Antibody Picoband&reg;Western blot analysis of Ceruloplasmin using anti-Ceruloplasmin antibody (PB9852). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin antigen affinity purified polyclonal antibody (PB9852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Ceruloplasmin at approximately 122 kDa. The expected band size for Ceruloplasmin is at 122 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9852-ceruloplasmin-primary-antibodies-fcm-testing-1.jpgAnti-Ceruloplasmin/CP Antibody Picoband&reg;Flow Cytometry analysis of HepG2 cells using anti-Ceruloplasmin antibody (PB9852). <br> Overlay histogram showing HepG2 cells stained with PB9852 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Ceruloplasmin Antibody (PB9852, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ceruloplasmin-picoband-trade-antibody-pb9853-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9853-ceruloplasmin-primary-antibodies-wb-testing-1.jpgAnti-Ceruloplasmin/CP Antibody Picoband&reg; Western blot analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (PB9853). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: mouse liver tissue lysates, <br> Lane 3: mouse HEPA1-6 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ceruloplasmin/CP antigen affinity purified polyclonal antibody (Catalog # PB9853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ceruloplasmin/CP at approximately 130 kDa. The expected band size for Ceruloplasmin/CP is at 121 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9853-cp-primary-antibodies-ihc-testing-4.jpgAnti-Ceruloplasmin/CP Antibody Picoband&reg;IHC analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (PB9853). <br>Ceruloplasmin/CP was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ceruloplasmin/CP Antibody (PB9853) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9853-2-IHC-anti-ceruloplasmin-picoband-antibody.jpgAnti-Ceruloplasmin/CP Antibody Picoband&reg; IHC analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (PB9853).<br> Ceruloplasmin/CP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ceruloplasmin/CP Antibody (PB9853) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9853-3-IHC-anti-ceruloplasmin-picoband-antibody.jpgAnti-Ceruloplasmin/CP Antibody Picoband&reg; IHC analysis of Ceruloplasmin/CP using anti-Ceruloplasmin/CP antibody (PB9853).<br> Ceruloplasmin/CP was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Ceruloplasmin/CP Antibody (PB9853) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-d-picoband-trade-antibody-pb9854-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9854-ctsd-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; Western blot analysis of CTSD using anti-CTSD antibody (PB9854). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTSD antigen affinity purified polyclonal antibody (Catalog # PB9854) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTSD at approximately 28, 45 kDa. The expected band size for CTSD is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9854-2-IHC-anti-cathepsin-d-picoband-antibody.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9854).<br> Cathepsin D was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9854) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9854-ctsd-primary-antibodies-fc-testing-3.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-CTSD antibody (PB9854). <br>Overlay histogram showing SiHa cells stained with PB9854 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTSD Antibody (PB9854&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9854-ctsd-primary-antibodies-if-testing-4.jpgAnti-Cathepsin D/CTSD Antibody Picoband&reg; IF analysis of CTSD using anti-CTSD antibody (PB9854). <br> CTSD was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CTSD Antibody (PB9854) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-g-picoband-trade-antibody-pb9855-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9855-cathepsin-g-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin G/CTSG Antibody Picoband&reg; Western blot analysis of Cathepsin G using anti-Cathepsin G antibody (PB9855). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat kidney tissue lysates, <br> Lane 3: mouse liver tissue lysates, <br> Lane 4: mouse kidney tissue lysates,<br> Lane 5: mouse HEPA1-6 whole cell lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G antigen affinity purified polyclonal antibody (Catalog # PB9855) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin G at approximately 29KD. The expected band size for Cathepsin G is at 29KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-k-picoband-trade-antibody-pb9856-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9856-1-WB-anti-cathepsin-k-picoband-antibody.jpgAnti-Cathepsin K/CTSK Antibody Picoband&reg; Western blot analysis of Cathepsin K using anti-Cathepsin K antibody (PB9856). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: 22RV1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin K antigen affinity purified polyclonal antibody (Catalog # PB9856) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin K at approximately 45 kDa. The expected band size for Cathepsin K is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9856-2-IHC-anti-cathepsin-k-picoband-antibody.jpgAnti-Cathepsin K/CTSK Antibody Picoband&reg; IHC analysis of Cathepsin K using anti-Cathepsin K antibody (PB9856). <br> Cathepsin K was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cathepsin K Antibody (PB9856) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9856-fcell-12-1503481-g002.jpgAnti-Cathepsin K/CTSK Antibody Picoband&reg;DFSC-EVs regulated tooth eruption by inhibiting osteoclast differentiation. (A) Schematic illustration of RAW264.7 and DFSC co-culture system. (B) Representative images of TRAP staining. Scale bar = 200 μm. (C) Quantitative analysis of TRAP-positive area. (D) The mRNA level of ACP5 , CTSK and CFOS in RAW264.7 cultured with DFSC. (E) The protein level of ACP5, CTSK and CFOS in RAW264.7 cultured with DFSC. (F) Western blotting quantification. (G) Schematic illustration of RAW264.7 and DFSC-EVs co-culture system. (H) Representative images of TRAP staining. Scale bar = 200 μm. (I) Quantitative analysis of TRAP-positive area. (J) The mRNA level of ACP5 , CTSK and CFOS in RAW264.7 cultured with DFSC-EVs. (K) The protein level of ACP5, CTSK and CFOS in RAW264.7 cultured with DFSC-EVs. (L) Western blotting quantification. ns, not significant. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9856-fcell-12-1503481-g003.jpgAnti-Cathepsin K/CTSK Antibody Picoband&reg;ANXA1 was the core factor of DFSC-EVs regulating osteoclast differentiation. (A) Gene ontology enrichment analysis of DFSC-EVs protein profiles. (B) The top proteins of Cadherin related to regulating osteoblast differentiation based on expression level. (C) The mRNA level of ANXA1 . (D) The protein level of ANXA1. (E) Western blotting quantification. (F) Schematic illustration of RAW264.7 and siANXA1-EVs co-culture system. (G) Representative images of TRAP staining. Scale bar = 200 μm. (H) Quantitative analysis of TRAP-positive area. (I) The mRNA level of ACP5 , CTSK and CFOS in RAW264.7 cultured with siANXA1-EVs. (J) The protein level of ACP5, CTSK and CFOS in RAW264.7 cultured with siANXA1-EVs. (K) Western blotting quantification. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9856-fcell-12-1503481-g004.jpgAnti-Cathepsin K/CTSK Antibody Picoband&reg;ANXA1 mediated PPARγ-CEBPα pathway to regulate osteoclast differentiation (A) The mRNA level of PPARγ in RAW264.7 cultured with siANXA1-EVs. (B) The mRNA level of CEBPα in RAW264.7 cultured with siANXA1-EVs. (C) The protein level of PPARγ and CEBPα in RAW264.7 cultured with siANXA1-EVs. (D) Quantitative analysis of PPARγ protein expression. (E) Quantitative analysis of CEBPα protein expression. (F) Schematic illustration of PPARγ inhibited RAW264.7 and DFSC-EVs co-culture system. (G) Representative images of TRAP staining. Scale bar = 200 μm. (H) Quantitative analysis of TRAP-positive area. (I) PPARγ inhibited RAW264.7 construction. (J) The mRNA level of CEBPα in PPARγ inhibited RAW264.7. (K) The protein level of PPARγ and CEBPα in PPARγ inhibited RAW264.7. (L) Quantitative analysis of PPARγ protein expression. (M) Quantitative analysis of CEBPα protein expression. (N) The mRNA level of ACP5 , CTSK and CFOS in PPARγ inhibited RAW264.7. (O) The protein level of ACP5, CTSK and CFOS in PPARγ inhibited RAW264.7. (P) Western blotting quantification. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1503481/full'>39834384</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9856-41598_2024_63837_fig6_html.pngAnti-Cathepsin K/CTSK Antibody Picoband&reg;Western blot validation of proteomic data. ( A ) Elevated levels of ITGB5, TNXB , CA II, CA III were observed in the peri-implant sclerosis samples, while elevated levels of ACP5 and CTSK were observed in femoral head necrosis samples. ( B ) The ratio of TIGAR/GAPDH intensities in Western blot. GAPDH was used as a loading control, quantified and normalized to GAPDH using ImageJ. All experiments were performed in triplicate, * indicates significant expression level change compared to the control group, *P < 0.05, **P < 0.01. ***P < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-63837-8'>38851808</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcl2-picoband-trade-antibody-pb9857-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9857-1-WB-anti-mip-2-antibody.jpgAnti-CXCL2 Antibody Picoband&reg; Western blot analysis of CXCL2 using anti-CXCL2 antibody (PB9857). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant human CXCL2 protein 0.5ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL2 antigen affinity purified polyclonal antibody (Catalog # PB9857) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL2 at approximately 11 kDa. The expected band size for CXCL2 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcl2-picoband-trade-antibody-pb9858-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9858-1-WB-anti-mip-2-antibody.jpgAnti-CXCL2 Antibody Picoband&reg; Western blot analysis of CXCL2 using anti-CXCL2 antibody (PB9858). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant rat CXCL2 protein 0.5ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL2 antigen affinity purified polyclonal antibody (Catalog # PB9858) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL2 at approximately 11 kDa. The expected band size for CXCL2 is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smac-diablo-picoband-trade-antibody-pb9860-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9860-diablo-primary-antibodies-wb-testing-1.jpgAnti-Smac/Diablo Antibody Picoband&reg;Western blot analysis of Smac/Diablo using anti-Smac/Diablo antibody (PB9860). <br> Electrophoresis was performed on a 12 SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: humna Jurkat whole cell lysates,<br> Lane 5: rat testis tissue lysates,<br> Lane 6: mouse testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Smac/Diablo antigen affinity purified polyclonal antibody (Catalog # PB9860) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Smac/Diablo at approximately 20 kDa. The expected band size for Smac/Diablo is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9860-diablo-primary-antibodies-ihc-testing-1.jpgAnti-Smac/Diablo Antibody Picoband&reg;IHC analysis of Smac/Diablo using anti-Smac/Diablo antibody (PB9860). <br> Smac/Diablo was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Smac/Diablo Antibody (PB9860) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9860-diablo-primary-antibodies-if-testing-1.jpgAnti-Smac/Diablo Antibody Picoband&reg;IF analysis of Smac/Diablo using anti-Smac/Diablo antibody (PB9860) and anti-Tubulin Alpha antibody (M03989-3).<br> Smac/Diablo was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Smac/Diablo Antibody (PB9860) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9860-diablo-primary-antibodies-ip-testing-1.jpgAnti-Smac/Diablo Antibody Picoband&reg;Immunoprecipitating (IP) Smac/Diablo in HepG2 whole cell lysate.<br> Western blot analysis of Smac/Diablo using anti-Smac/Diablo antibody (PB9860); <br> Lane 1: HepG2 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-Smac/Diablo antibody in HepG2 whole cell lysate;<br> Lane 3: anti-Smac/Diablo antibody (2μg) + HepG2 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Smac/Diablo antigen affinity purified polyclonal antibody (PB9860) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for Smac/Diablo at approximately 20 kDa. The expected band size for Smac/Diablo is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9860-diablo-primary-antibodies-fcm-testing-1.jpgAnti-Smac/Diablo Antibody Picoband&reg;Flow Cytometry analysis of 293T cells using anti-Smac/Diablo antibody (PB9860). <br> Overlay histogram showing 293T cells stained with PB9860 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Smac/Diablo Antibody (PB9860, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcl5-picoband-trade-antibody-pb9859-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9859-1-WB-anti-cxcl5-ena-78-antibody.jpgAnti-CXCL5 Antibody Picoband&reg; Western blot analysis of CXCL5 using anti-CXCL5 antibody (PB9859). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. <br> Lane 1: recombinant rat CXCL5 protein 0.5ng.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL5 antigen affinity purified polyclonal antibody (Catalog # PB9859) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL5 at approximately 14 kDa. The expected band size for CXCL5 is at 14 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egf-picoband-trade-antibody-pb9861-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9861-1-WB-anti-egf-picoband-antibody.jpgAnti-EGF Antibody Picoband&reg; Western blot analysis of EGF using anti-EGF antibody (PB9861). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: 22RV1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (Catalog # PB9861) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF at approximately 134 kDa. The expected band size for EGF is at 133 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egf-picoband-trade-antibody-pb9862-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9862-1-WB-anti-egf-picoband-antibody.jpgAnti-EGF Antibody Picoband&reg; Western blot analysis of EGF using anti-EGF antibody (PB9862). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGF antigen affinity purified polyclonal antibody (Catalog # PB9862) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGF at approximately 134 kDa. The expected band size for EGF is at 133 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-egf-picoband-trade-antibody-pb9863-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9863-egfr-primary-antibodies-wb-testing-1.jpgAnti-EGFR Antibody Picoband&reg; Western blot analysis of EGFR using anti-EGFR antibody (PB9863). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human U87 whole cell lysates, <br> Lane 4: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody (Catalog # PB9863) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR at approximately 175 kDa. The expected band size for EGFR is at 134 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fasn-picoband-trade-antibody-pb9865-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9865-fasn-primary-antibodies-wb-testing-1.jpgAnti-Fatty Acid Synthase/FASN Antibody Picoband&reg; Western blot analysis of FASN using anti-FASN antibody (PB9865). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: human MCF-7 whole cell lysates,<br> Lane 6: human RT4 whole cell lysates,<br> Lane 7: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN antigen affinity purified polyclonal antibody (Catalog # PB9865) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FASN at approximately 273 kDa. The expected band size for FASN is at 273 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9865-fasn-primary-antibodies-wb-testing-2.jpgAnti-Fatty Acid Synthase/FASN Antibody Picoband&reg; Western blot analysis of FASN using anti-FASN antibody (PB9865). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: mouse lung tissue lysates,<br> Lane 3: mouse liver tissue lysates,<br> Lane 4: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN antigen affinity purified polyclonal antibody (Catalog # PB9865) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FASN at approximately 273 kDa. The expected band size for FASN is at 273 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9865-3-ihc-anti-fasn-fatty-acid-synthase-picoband-antibody.jpgAnti-Fatty Acid Synthase/FASN Antibody Picoband&reg; IHC analysis of FASN using anti-FASN antibody (PB9865). <br> FASN was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FASN Antibody (PB9865) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9865-fasn-primary-antibodies-if-testing-4.jpgAnti-Fatty Acid Synthase/FASN Antibody Picoband&reg; IF analysis of FASN using anti-FASN antibody (PB9865). <br> FASN was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FASN Antibody (PB9865) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9865-fasn-primary-antibodies-if-testing-5_1.jpgAnti-Fatty Acid Synthase/FASN Antibody Picoband&reg; IF analysis of FASN using anti-FASN antibody (PB9865). <br> FASN was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-FASN Antibody (PB9865) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9865-fasn-primary-antibodies-if-testing-6.jpgAnti-Fatty Acid Synthase/FASN Antibody Picoband&reg; IF analysis of FASN using anti-FASN antibody (PB9865). <br> FASN was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FASN Antibody (PB9865) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fcrn-fcgrt-picoband-trade-antibody-pb9866-boster.html2026-03-24T05:05:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9866-1-WB-anti-fcrn-fcgrt-picoband-antibody.jpgAnti-FCGRT Antibody Picoband&reg; Western blot analysis of FCGRT using anti-FCGRT antibody (PB9866). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGRT antigen affinity purified polyclonal antibody (Catalog # PB9866) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGRT at approximately 50 kDa. The expected band size for FCGRT is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9866-2-IHC-anti-fcrn-fcgrt-picoband-antibody.jpgAnti-FCGRT Antibody Picoband&reg; IHC analysis of FCGRT using anti-FCGRT antibody (PB9866). <br> FCGRT was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FCGRT Antibody (PB9866) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fcrn-fcgrt-picoband-trade-antibody-pb9867-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9867-1-WB-anti-fcrn-fcgrt-picoband-antibody.jpgAnti-FCGRT Antibody Picoband&reg; Western blot analysis of FCGRT using anti-FCGRT antibody (PB9867). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGRT antigen affinity purified polyclonal antibody (Catalog # PB9867) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGRT at approximately 50 kDa. The expected band size for FCGRT is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9867-2-IHC-anti-fcrn-fcgrt-picoband-antibody.jpgAnti-FCGRT Antibody Picoband&reg; IHC analysis of FCGRT using anti-FCGRT antibody (PB9867). <br> FCGRT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FCGRT Antibody (PB9867) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf23-picoband-trade-antibody-pb9868-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9868-1-WB-anti-fgf23-picoband-antibody.jpgAnti-FGF23 Antibody Picoband&reg; Western blot analysis of FGF 23 using anti-FGF 23 antibody (PB9868). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF 23 antigen affinity purified polyclonal antibody (Catalog # PB9868) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF 23 at approximately 34 kDa. The expected band size for FGF 23 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vegfd-picoband-trade-antibody-pb9869-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/v/e/vegfd-primary-antibodies-pb9869-wb-testing-1.jpgAnti-VEGFD/FIGF Antibody Picoband&reg;<b> Western blot analysis of VEGFD using anti-VEGFD antibody (PB9869). </b><br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates&#44;<br> Lane 2: rat brain tissue lysates&#44;<br> Lane 3: mouse lung tissue lysates&#44;<br> Lane 4: mouse brain tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFD antigen affinity purified polyclonal antibody (Catalog # PB9869) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFD at approximately 40KD. The expected band size for VEGFD is at 40KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/v/e/vegfd-primary-antibodies-pb9869-ihc-testing-2.jpgAnti-VEGFD/FIGF Antibody Picoband&reg;<b> IHC analysis of VEGFD using anti-VEGFD antibody (PB9869).</b><br> VEGFD was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VEGFD Antibody (PB9869) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/v/e/vegfd-primary-antibodies-pb9869-ihc-testing-3.jpgAnti-VEGFD/FIGF Antibody Picoband&reg;<b> IHC analysis of VEGFD using anti-VEGFD antibody (PB9869).</b><br> VEGFD was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VEGFD Antibody (PB9869) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/v/e/vegfd-primary-antibodies-pb9869-ihc-testing-4.jpgAnti-VEGFD/FIGF Antibody Picoband&reg;<b> IHC analysis of VEGFD using anti-VEGFD antibody (PB9869).</b><br> VEGFD was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-VEGFD Antibody (PB9869) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-filaggrin-picoband-trade-antibody-pb9870-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9870-1-WB-anti-filaggrin-picoband-antibody.jpgAnti-Filaggrin/FLG Antibody Picoband&reg; Western blot analysis of Filaggrin using anti-Filaggrin antibody (PB9870). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: 22RV1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filaggrin antigen affinity purified polyclonal antibody (Catalog # PB9870) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filaggrin at approximately 435 kDa. The expected band size for Filaggrin is at 435 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9870-2-IHC-anti-filaggrin-picoband-antibody.jpgAnti-Filaggrin/FLG Antibody Picoband&reg; IHC analysis of Filaggrin using anti-Filaggrin antibody (PB9870). <br> Filaggrin was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Filaggrin Antibody (PB9870) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9870-fbioe-08-00388-g005.jpgAnti-Filaggrin/FLG Antibody Picoband&reg;Immunofluorescence staining of filaggrin, CK10, CK14, Ki67, vimentin, perilipin A, and DAPI of native skin and skin models. Cell nuclei (stained with DAPI) are illustrated in white, characteristic proteins (stained with respective specific antibody) in green. White dashed line shows epidermal-dermal border. Scale bar: 50 μm.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00388/full'>32457884</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il7-picoband-trade-antibody-pb9871-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9871-1-WB-anti-il-7-picoband-antibody.jpgAnti-IL7 Antibody Picoband&reg; Western blot analysis of IL7 using anti-IL7 antibody (PB9871). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse brain tissue lysates,<br> Lane 2: mouse thymus tissue lysates,<br> Lane 3: NIH3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7 antigen affinity purified polyclonal antibody (Catalog # PB9871) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7 at approximately 20 kDa. The expected band size for IL7 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9871-il7-primary-antibodies-ihc-testing-2.jpgAnti-IL7 Antibody Picoband&reg; IHC analysis of IL7 using anti-IL7 antibody (PB9871). <br> IL7 was detected in a paraffin-embedded section of mouse lymph nodes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL7 Antibody (PB9871) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9871-il7-primary-antibodies-ihc-testing-3.jpgAnti-IL7 Antibody Picoband&reg; IHC analysis of IL7 using anti-IL7 antibody (PB9871). <br> IL7 was detected in a paraffin-embedded section of rat lymph nodes tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL7 Antibody (PB9871) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mdmx-picoband-trade-antibody-pb9872-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9872-1-WB-anti-mdmx-picoband-antibody.jpgAnti-MDMX/MDM4 Antibody Picoband&reg; Western blot analysis of MDMX using anti-MDMX antibody (PB9872). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse testis tissue lysates,<br> Lane 2: 22RV1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDMX antigen affinity purified polyclonal antibody (Catalog # PB9872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDMX at approximately 75 kDa. The expected band size for MDMX is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9872-2-IHC-anti-mdmx-picoband-antibody.jpgAnti-MDMX/MDM4 Antibody Picoband&reg; IHC analysis of MDMX using anti-MDMX antibody (PB9872). <br> MDMX was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MDMX Antibody (PB9872) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mga-picoband-trade-antibody-pb9873-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9873-1-WB-anti-mga-picoband-antibody.jpgAnti-MGA Antibody Picoband&reg; Western blot analysis of MGA using anti-MGA antibody (PB9873). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: MCF-7 whole cell lysates,<br> Lane 3: SW620 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGA antigen affinity purified polyclonal antibody (Catalog # PB9873) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGA at approximately 334 kDa. The expected band size for MGA is at 336 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nestin-picoband-trade-antibody-pb9874-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9874-1-WB-anti-nestin-picoband-antibody.jpgAnti-Nestin Antibody Picoband&reg; Western blot analysis of Nestin using anti-Nestin antibody (PB9874). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: HEPG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nestin antigen affinity purified polyclonal antibody (Catalog # PB9874) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nestin at approximately 230 kDa. The expected band size for Nestin is at 177 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9874-2-IHC-anti-nestin-picoband-antibody.jpgAnti-Nestin Antibody Picoband&reg; IHC analysis of Nestin using anti-Nestin antibody (PB9874). <br> Nestin was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nestin Antibody (PB9874) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9874-nestin-primary-antibodies-ihc-testing-1.jpgAnti-Nestin Antibody Picoband&reg;IHC analysis of Nestin using anti-Nestin antibody (PB9874). <br> Nestin was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nestin Antibody (PB9874) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9874-nrr-8-427-g002.jpgAnti-Nestin Antibody Picoband&reg;Expression of neural proteins in cells induced by sonic hedgehog and fibroblast growth factor 8 for 12 days (immunocytochemical staining, × 400). Cells were positive for nestin (A), neuron-specific enolase (B), microtubule-associated protein 2 (C), tyrosine hydroxylase (E) and vesicular monoamine transporter-2 (F), but negative for glial fibrillary acid protein (D) after induction with sonic hedgehog + fibroblast growth factor 8 for 12 days. Arrows represent positive cells.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4146134/&orig_host=www.ncbi.nlm.nih.gov'>25206684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9874-nrr-8-427-g003.jpgAnti-Nestin Antibody Picoband&reg;Overall expression of nestin, NSE, MAP2, GFAP, TH and VMAT2 in induced rat bone marrow-derived mesenchymal stem cells under different conditions. Expression rate (%) = number of positive cells/total number of cells × 100%. Data were determined by immunocytochemistry and expressed as mean ± SEM. Statistical differences were tested using F -test. a P < 0.05, vs . control group; b P < 0.05, vs . Xiangdan injection group and ATRA + GDNF group. NSE: Neuron-specific enolase; MAP2: microtubule- associated protein 2; GFAP: glial fibrillary acid protein; TH: tyrosine hydroxylase; VMAT2: vesicular monoamine transporter-2; group A: Xiangdan injection; group B: all-trans retinoic acid + glial cell line-derived neurotrophic factor; group C: sonic hedgehog + fibroblast growth factor 8.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC4146134/&orig_host=www.ncbi.nlm.nih.gov'>25206684</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9874-3-IHC-anti-nestin-picoband-antibody.jpgAnti-Nestin Antibody Picoband&reg; IHC analysis of Nestin using anti-Nestin antibody (PB9874). <br> Nestin was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nestin Antibody (PB9874) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9874-nestin-primary-antibodies-ihc-testing-4.jpgAnti-Nestin Antibody Picoband&reg; IHC analysis of Nestin using anti-Nestin antibody (PB9874). <br> Nestin was detected in frozen section of mouse kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Nestin Antibody (PB9874) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9874-nestin-primary-antibodies-ihc-testing-5.jpgAnti-Nestin Antibody Picoband&reg; IHC analysis of Nestin using anti-Nestin antibody (PB9874). <br> Nestin was detected in frozen section of rat kidney tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Nestin Antibody (PB9874) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9874_nestin.pngAnti-Nestin Antibody Picoband&reg; IHC analysis of Nestin using anti-Nestin antibody (PB9874). <br> Nestin was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nestin Antibody (PB9874) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nmt55-p54nrb-picoband-trade-antibody-pb9875-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-wb-testing-1.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; Western blot analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human SW620 whole cell lysates, <br> Lane 4: human PANC-1 whole cell lysates, <br> Lane 5: human U20S whole cell lysates, <br> Lane 6: rat lung tissue lysates,<br> Lane 7: mouse lung tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nmt55/p54nrb antigen affinity purified polyclonal antibody (Catalog # PB9875) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nmt55/p54nrb at approximately 60KD. The expected band size for nmt55/p54nrb is at 60KD. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-ihc-testing-2.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IHC analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-ihc-testing-3.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IHC analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-ihc-testing-4.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IHC analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-ihc-testing-5.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IHC analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-ihc-testing-6.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IHC analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-ihc-testing-7.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IHC analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-if-testing-8.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IF analysis of nmt55/p54nrbusing anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594 Conjugated Avidin (BA1142). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-if-testing-9.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IF analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-if-testing-10.jpgAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; IF analysis of nmt55/p54nrb using anti-nmt55/p54nrb antibody (PB9875). <br> nmt55/p54nrb was detected in immunocytochemical section of SKOV-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-nmt55/p54nrb Antibody (PB9875) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9875-nmt55-p54nrb-primary-antibodies-fcm-testing-11.pngAnti-nmt55/p54nrb/NONO Antibody Picoband&reg; Flow Cytometry analysis of HELA cells using anti-nmt55/p54nrb antibody (PB9875). <br>Overlay histogram showing HELA cells stained with PB9875 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-nmt55/p54nrb Antibody (PB9875, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nucleobindin-2-picoband-trade-antibody-pb9876-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9876-1-WB-anti-nesfatin-1-picoband-antibody.jpgAnti-Nucleobindin 2/NUCB2 Antibody Picoband&reg; Western blot analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody (PB9876). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HEPA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleobindin 2 antigen affinity purified polyclonal antibody (Catalog # PB9876) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nucleobindin 2 at approximately 55 kDa. The expected band size for Nucleobindin 2 is at 50 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9876-2-IHC-anti-nesfatin-1-picoband-antibody.jpgAnti-Nucleobindin 2/NUCB2 Antibody Picoband&reg; IHC analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody (PB9876). <br> Nucleobindin 2 was detected in a paraffin-embedded section of mouse gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleobindin 2 Antibody (PB9876) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9876-3-IHC-anti-nesfatin-1-picoband-antibody.jpgAnti-Nucleobindin 2/NUCB2 Antibody Picoband&reg; IHC analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody (PB9876). <br> Nucleobindin 2 was detected in a paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Nucleobindin 2 Antibody (PB9876) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pak3-picoband-trade-antibody-pb9877-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9877-pak3-primary-antibodies-wb-testing-1.jpgAnti-PAK3 Antibody Picoband&reg;Western blot analysis of PAK3 using anti-PAK3 antibody (PB9877). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK3 antigen affinity purified polyclonal antibody (PB9877) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK3 at approximately 62 kDa. The expected band size for PAK3 is at 62 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nap2-picoband-trade-antibody-pb9878-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9878-1-WB-anti-nap2-picoband-antibody.jpgAnti-NAP2/PPBP Antibody Picoband&reg; Western blot analysis of NAP2 using anti-NAP2 antibody (PB9878). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NAP2 antigen affinity purified polyclonal antibody (Catalog # PB9878) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NAP2 at approximately 19 kDa. The expected band size for NAP2 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9878-2-IHC-anti-nap2-picoband-antibody.jpgAnti-NAP2/PPBP Antibody Picoband&reg; IHC analysis of NAP2 using anti-NAP2 antibody (PB9878). <br> NAP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NAP2 Antibody (PB9878) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9878-cxcl7-primary-antibodies-elisa-testing-3.jpgAnti-NAP2/PPBP Antibody Picoband&reg; Sandwich ELISA - Recombinant human CXCL7 protein standard curve.<br> Use in combination with reagents from Human CXCL7 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0729). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-darpp32-picoband-trade-antibody-pb9879-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9879-darpp32-primary-antibodies-wb-testing-1.jpgAnti-DARPP32/PPP1R1B Antibody Picoband&reg; Western blot analysis of DARPP32 using anti-DARPP32 antibody (PB9879). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human CACO-2 whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DARPP32 antigen affinity purified polyclonal antibody (Catalog # PB9879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DARPP32 at approximately 32 kDa. The expected band size for DARPP32 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9879-2-IHC-anti-darpp32-picoband-antibody.jpgAnti-DARPP32/PPP1R1B Antibody Picoband&reg; IHC analysis of DARPP32 using anti-DARPP32 antibody (PB9879). <br> DARPP32 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DARPP32 Antibody (PB9879) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9879-3-IHC-anti-darpp32-picoband-antibody.jpgAnti-DARPP32/PPP1R1B Antibody Picoband&reg; IHC analysis of DARPP32 using anti-DARPP32 antibody (PB9879). <br> DARPP32 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DARPP32 Antibody (PB9879) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9879-4-IHC-anti-darpp32-picoband-antibody.jpgAnti-DARPP32/PPP1R1B Antibody Picoband&reg; IHC analysis of DARPP32 using anti-DARPP32 antibody (PB9879). <br> DARPP32 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DARPP32 Antibody (PB9879) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9879-5.pngAnti-DARPP32/PPP1R1B Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-DARPP32 antibody (PB9879). <br> Overlay histogram showing THP-1 cells stained with PB9879 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DARPP32 Antibody (PB9879&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9879-6.pngAnti-DARPP32/PPP1R1B Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-DARPP32 antibody (PB9879). <br> Overlay histogram showing PC-3 cells stained with PB9879 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DARPP32 Antibody (PB9879&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-epcr-cd201-picoband-trade-antibody-pb9880-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9880-1-WB-anti-epcr-picoband-antibody.jpgAnti-EPCR/CD201/PROCR Antibody Picoband&reg; Western blot analysis of EPCR/CD201 using anti-EPCR/CD201 antibody (PB9880). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCR/CD201 antigen affinity purified polyclonal antibody (Catalog # PB9880) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCR/CD201 at approximately 46 kDa, 52 kDa. The expected band size for EPCR/CD201 is at 27 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9880-epcr-primary-antibodies-elisa-testing-2.jpgAnti-EPCR/CD201/PROCR Antibody Picoband&reg; Sandwich ELISA - Recombinant human EPCR protein standard curve.<br> Use in combination with reagents from Human EPCR ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0957). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prokineticin-1-picoband-trade-antibody-pb9882-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9882-1_1.jpgAnti-Prokineticin 1/PROK1 Antibody Picoband&reg; Western blot analysis of Prokineticin 1 using anti-Prokineticin 1 antibody (PB9882). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates,<br> Lane 2: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prokineticin 1 antigen affinity purified polyclonal antibody (Catalog # PB9882) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prokineticin 1 at approximately 12 kDa. The expected band size for Prokineticin 1 is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prokineticin-1-picoband-trade-antibody-pb9881-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9881-1-WB-anti-eg-vegf-picoband-antibody.jpgAnti-Prokineticin 1/PROK1 Antibody Picoband&reg; Western blot analysis of Prokineticin 1 using anti-Prokineticin 1 antibody (PB9881). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prokineticin 1 antigen affinity purified polyclonal antibody (Catalog # PB9881) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prokineticin 1 at approximately 20 kDa. The expected band size for Prokineticin 1 is at 12 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-prokineticin-1-picoband-trade-antibody-pb9883-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9883-rab7-primary-antibodies-wb-testing-1.jpgAnti-RAB7/RAB7A Antibody Picoband&reg; Western blot analysis of RAB7 using anti-RAB7 antibody (PB9883). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human A375 whole cell lysates,<br> Lane 3: huamn A431 whole cell lysates,<br> Lane 4: human HL-60 whole cell lysates,<br> Lane 5: human U87 whole cell lysates,<br> Lane 6: human MCF-7 whole cell lysates.<br> Lane 7: human THP-1 whole cell lysates,<br> Lane 8: rat brain tissue lysates,<br> Lane 9: rat lung tissue lysates,<br> Lane 10: mouse brain tissue lysates,<br> Lane 11: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7 antigen affinity purified polyclonal antibody (Catalog # PB9883) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB7 at approximately 23 kDa. The expected band size for RAB7 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9883-2.jpgAnti-RAB7/RAB7A Antibody Picoband&reg; IF analysis of RAB7 using anti-RAB7 antibody (PB9883). <br> RAB7 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RAB7 Antibody (PB9883) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9883-3.jpgAnti-RAB7/RAB7A Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-RAB7 antibody (PB9883).<br>Overlay histogram showing A431 cells stained with PB9883 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB7 Antibody (PB9883,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9883-4.jpgAnti-RAB7/RAB7A Antibody Picoband&reg; IF analysis of RAB7 using anti-RAB7 antibody (PB9883). <br> RAB7 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RAB7 Antibody (PB9883) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rab9-picoband-trade-antibody-pb9884-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9884-rab9a-primary-antibodies-wb-testing-1.jpgAnti-Rab9/RAB9A Antibody Picoband&reg; Western blot analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (PB9884). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: monkey COS-7 whole cell lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab9/RAB9A antigen affinity purified polyclonal antibody (Catalog # PB9884) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab9/RAB9A at approximately 23 kDa. The expected band size for Rab9/RAB9A is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9884-rab9a-primary-antibodies-if-testing-2.jpgAnti-Rab9/RAB9A Antibody Picoband&reg; IF analysis of Rab9/RAB9A using anti-Rab9/RAB9A antibody (PB9884). <br> Rab9/RAB9A was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Rab9/RAB9A Antibody (PB9884) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9884-rab9a-primary-antibodies-fcm-testing-3.jpgAnti-Rab9/RAB9A Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Rab9/RAB9A antibody (PB9884). <br>Overlay histogram showing U20S cells stained with PB9884 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rab9/RAB9A Antibody (PB9884, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rnh1-picoband-trade-antibody-pb9885-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-1_2_.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; Western blot analysis of RNH1 using anti-RNH1 antibody (PB9885). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates&#44; <br> Lane 2: human Hela whole cell lysates&#44; <br> Lane 3: human SK-OV-3 whole cell lysates&#44; <br> Lane 4: human Jurkat whole cell lysates&#44; <br> Lane 5: human U-87MG whole cell lysates&#44; <br> Lane 6: monkey COS-7 whole cell lysates&#44; <br> Lane 7: human SW620 whole cell lysates&#44; <br> Lane 8: human Caco-2 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNH1 antigen affinity purified polyclonal antibody (Catalog # PB9885) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNH1 at approximately 50KD. The expected band size for RNH1 is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-2_1.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; Western blot analysis of RNH1 using anti-RNH1 antibody (PB9885). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: rat testis tissue lysates&#44; <br> Lane 3: rat lung tissue lysates&#44; <br> Lane 4: mouse brain tissue lysates&#44; <br> Lane 5: mouse testis tissue lysates&#44; <br> Lane 6: mouse lung tissue lysates, <br>Lane 7: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNH1 antigen affinity purified polyclonal antibody (Catalog # PB9885) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNH1 at approximately 50KD. The expected band size for RNH1 is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-3.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; IHC analysis of RNH1 using anti-RNH1 antibody (PB9885). <br> RNH1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNH1 Antibody (PB9885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-4.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; IHC analysis of RNH1 using anti-RNH1 antibody (PB9885). <br> RNH1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNH1 Antibody (PB9885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-5.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; IHC analysis of RNH1 using anti-RNH1 antibody (PB9885). <br> RNH1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNH1 Antibody (PB9885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-6.jpgAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; IHC analysis of RNH1 using anti-RNH1 antibody (PB9885). <br> RNH1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RNH1 Antibody (PB9885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-7.pngAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-RNH1 antibody (PB9885). <br> Overlay histogram showing A549 cells stained with PB9885 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNH1 Antibody (PB9885&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9885-8.pngAnti-Ribonuclease Inhibitor/RNH1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-RNH1 antibody (PB9885). <br> Overlay histogram showing A549 cells stained with PB9885 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNH1 Antibody (PB9885&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rpa70-picoband-trade-antibody-pb9886-boster.html2026-03-24T05:05:27+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9886-rpa70-primary-antibodies-wb-testing-1.jpgAnti-RPA70/RPA1 Antibody Picoband&reg; Western blot analysis of RPA70 using anti-RPA70 antibody (PB9886). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA70 antigen affinity purified polyclonal antibody (Catalog # PB9886) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPA70 at approximately 70 kDa. The expected band size for RPA70 is at 68 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9886-rpa70-primary-antibodies-if-testing-2.jpgAnti-RPA70/RPA1 Antibody Picoband&reg; IF analysis of RPA70 using anti-RPA70 antibody (PB9886). <br> RPA70 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPA70 Antibody (PB9886) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9886-rpa70-primary-antibodies-fcm-testing-3.jpgAnti-RPA70/RPA1 Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-RPA70 antibody (PB9886). <br>Overlay histogram showing U251 cells stained with PB9886 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPA70 Antibody (PB9886, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-scn1a-picoband-trade-antibody-pb9887-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9887-1-WB-anti-scn1a-picoband-antibody.jpgAnti-Scn1a Antibody Picoband&reg; Western blot analysis of Scn1a using anti-Scn1a antibody (PB9887). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates,<br> Lane 3: U87 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scn1a antigen affinity purified polyclonal antibody (Catalog # PB9887) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scn1a at approximately 250 kDa. The expected band size for Scn1a is at 229 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9887-41413_2022_204_fig6_html.pngAnti-Scn1a Antibody Picoband&reg;The sodium channel Scn1a mediates plasma membrane depolarization in senescent preosteoblasts. a mRNA expression of voltage-sensitive sodium channels in the Δ Tsc1 and control calvarial preosteoblasts indicated by our previous global mRNA expression profile (GSE74781). b Binding site of C/EBPα in the 5′-UE of Scn1a (yellow box) predicted by the JASPAR database ( ). rs: JASPAR relative scores, which are defined as 1 for the maximum-likelihood sequence. Boxes represent exons: blue coding exons, red noncoding exons conserved between humans and mice, white noncoding exons identified in either human or mouse transcripts. Noncoding exons are named alphabetically, and the first coding exon (exon 1) of the Scn1a gene is indicated. Genomic distances between exons are indicated. c Binding of C/EBPα to the 5′-UE sequence of Scn1a in vivo, determined by a ChIP assay using the Δ Tsc1 and Δ Raptor cells and anti-C/EBPα antibody or IgG. The ChIP samples were then subjected to qPCR with the Scn1a 5′-UE primers. The percentage of the input of the sample by using the anti-C/EBPα antibody in the wild-type cells was normalized to 100. The Δ Tsc1 osteoblasts were transfected with Scn1a siRNA and subjected to ( d ) Scn1a detection with western blotting, ( e ) measurement of relative plasma membrane potential with DiBAC4 dye, ( f ) SA-β-gal staining and quantification of the proportion of SA-β-gal-positive cells, ( g ) immunostaining of EdU and quantitative analysis of EdU + cells relative to total cells, and ( h ) qPCR analysis of IL-6 and Cxcl1 mRNA. Double immunostaining of Osx plus Scn1a ( i ) and Osx plus p16 ( j ) in the tibias of the 18-month-old Δ Tsc1 mice injected with adenovirus encoding si-Scn1a for 1 month. Double positively stained cells were quantified. Representative μCT images ( k ) and quantification of trabecular bone ( l ) in the mice. ( m ) Representative images of calcein labels and quantification of the mineral apposition rate (MAR) in femurs from the 18-month-old Δ Tsc1 mice receiving si-Scn1a. Scale bars: 50 μm in e , g , m ; 100 μm in i , j ; and 500 μm in k . Data are shown as the mean ± SD. The numbers of samples ( n ) are indicated in each figure panel. P values were determined with two-tailed Student’s t test for single comparisons <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41413-022-00204-1'>35256591</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9887-41413_2022_204_fig7_html.pngAnti-Scn1a Antibody Picoband&reg;Prosenescent stress activates Scn1a by inhibiting PKA. a Replicative wild-type (WT), Δ Tsc1 and Δ Raptor calvarial osteoblasts were incubated with the fluorescent DiBAC4 dye and photographed under a confocal microscope. Scale bar, 50 μm. Relative plasma membrane potentials were measured. ROS-induced senescent wild-type preosteoblasts were treated with F/I (forskolin + IBMx, PKA activator) or left untreated. The cells were subjected to ( b ) measurement of total cellular PKA activities, ( c ) DiBAC4 staining and measurement of relative plasma membrane potentials. Scale bar, 50 μm. d SA-β-gal staining of cells in b and quantification of the proportion of SA-β-gal-positive cells in each population. Scale bar, 100 μm. Replicative WT and Δ Tsc1 osteoblasts were treated with H-89 (PKA inhibitor) and were subjected to ( e ) measurement of total cellular PKA activities, ( f ) DiBAC4 staining and measurement of relative plasma membrane potentials. Scale bar, 50 μm. g SA-β-gal staining of cells in e and quantification of the proportion of SA-β-gal-positive cells in each population. Scale bar, 100 μm. h Immunostaining of EdU in cells in e and quantitative analysis of EdU + cells relative to total cells. Scale bar, 100 μm. Data are shown as the mean ± SD. The numbers of samples ( n ) are indicated in each figure panel. P values were determined by two-tailed Student’s t test for single comparisons <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41413-022-00204-1'>35256591</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9887-41419_2019_1979_fig3_html.pngAnti-Scn1a Antibody Picoband&reg;BIL elevated the expression level of SCN1A in MVN neurons. a , b Immunofluorescence labeling images of nuclei (blue), NeuN (green), and SCN1A (red) of control ( a ) and BIL group ( b ). Neurons with co-labeling of NeuN and SCN1A were chosen as AOI, and the mean fluorescence intensities of SCN1A from AOI were quantified in artificial units (AUs). c Statistical comparison showing larger MISCN1A in the BIL group, implicating elevated expression level of Nav1.1 after BIL treatment. *** p < 0.001, independent-samples t- test. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41419-019-1979-1'>31601780</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9887-41419_2019_1979_fig4_html.pngAnti-Scn1a Antibody Picoband&reg;Ca 2+ was required for BIL-induced upregulation of activity and level of VGSCs. a An example recording showing that neither the frequency of spontaneous firings nor the spike waveform of a MVN neuron was altered in slices preincubated in BAPTA-AM (40 μM, 30 min). b , c Statistical results showing no significant change in spike frequency, ratio of I inward / I outward . d – f MI SCN1A was not statistically different between control and BIL groups ( d ) as exemplified by the immunofluorescence staining of nuclei (blue), NeuN (green), and SCN1A (red) in control ( e ) and BIL groups ( f ). NS no statistical difference, independent-samples t test. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41419-019-1979-1'>31601780</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9887-41419_2019_1979_fig5_html.pngAnti-Scn1a Antibody Picoband&reg;Blocking exocytosis with TAT-NSF700 precluded the effects of BIL on MVN neurons. a – c Example recording traces ( a ) showing that when the slice was preincubated with TAT-NSF700 (5 μM, 30 min), a permeable thrombin-induced exocytosis inhibitor, neither the discharge frequency of MVN neuron ( b ) nor the ratio of I inward / I outward ( c ) was altered by BIL (3 μM, 3 min). d–f MI SCN1A was not statistically different between control and BIL groups ( d ) in slices pretreated with TAT-NSF700, as illustrated by the immunofluorescence images comparing staining of nuclei (blue), NeuN (green), and SCN1A (red) in control ( e ) and BIL group ( f ). NS no statistical difference, independent-samples t- test. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.nature.com/articles/s41419-019-1979-1'>31601780</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sfrp4-picoband-trade-antibody-pb9888-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9888-1_1.jpgAnti-SFRP4 Antibody Picoband&reg; Western blot analysis of SFRP4 using anti-SFRP4 antibody (PB9888). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: A549 whole cell lysates,<br> Lane 2: SW620 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP4 antigen affinity purified polyclonal antibody (Catalog # PB9888) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP4 at approximately 49 kDa. The expected band size for SFRP4 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9888-2-IHC-anti-sfrp4-picoband-antibody.jpgAnti-SFRP4 Antibody Picoband&reg; IHC analysis of SFRP4 using anti-SFRP4 antibody (PB9888). <br> SFRP4 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SFRP4 Antibody (PB9888) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-six1-picoband-trade-antibody-pb9889-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9889-1-WB-anti-six1-picoband-antibody.jpgAnti-SIX1 Antibody Picoband&reg; Western blot analysis of SIX1 using anti-SIX1 antibody (PB9889). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates,<br> Lane 2: 22RV1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIX1 antigen affinity purified polyclonal antibody (Catalog # PB9889) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIX1 at approximately 37 kDa. The expected band size for SIX1 is at 32 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-skap55-picoband-trade-antibody-pb9890-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9890-1-WB-anti-skap55-picoband-antibody.jpgAnti-SKAP55/SKAP1 Antibody Picoband&reg; Western blot analysis of SKAP55 using anti-SKAP55 antibody (PB9890). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: PANC whole cell lysates,<br> Lane 2: JURKAT whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKAP55 antigen affinity purified polyclonal antibody (Catalog # PB9890) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKAP55 at approximately 60 kDa. The expected band size for SKAP55 is at 41 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9890-2-IHC-anti-skap55-picoband-antibody.jpgAnti-SKAP55/SKAP1 Antibody Picoband&reg; IHC analysis of SKAP55 using anti-SKAP55 antibody (PB9890). <br> SKAP55 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SKAP55 Antibody (PB9890) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9890-skap1-primary-antibodies-ihc-testing-3.jpgAnti-SKAP55/SKAP1 Antibody Picoband&reg;IHC analysis of SKAP1 using anti-SKAP1 antibody (PB9890). <br>SKAP1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SKAP1 Antibody (PB9890) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-glut9-picoband-trade-antibody-pb9891-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9891-1-WB-anti-glut9-picoband-antibody.jpgAnti-GLUT9/SLC2A9 Antibody Picoband&reg; Western blot analysis of GLUT9 using anti-GLUT9 antibody (PB9891). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HepG2 whole cell lysates,<br> Lane 2: A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT9 antigen affinity purified polyclonal antibody (Catalog # PB9891) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT9 at approximately 55 kDa. The expected band size for GLUT9 is at 59 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9891-2.jpgAnti-GLUT9/SLC2A9 Antibody Picoband&reg; IHC analysis of GLUT9 using anti-GLUT9 antibody (PB9891). <br> GLUT9 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GLUT9 Antibody (PB9891) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9891-3.jpgAnti-GLUT9/SLC2A9 Antibody Picoband&reg; IF analysis of GLUT9 using anti-GLUT9 antibody (PB9891). <br> GLUT9 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-GLUT9 Antibody (PB9891) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9891-4.jpgAnti-GLUT9/SLC2A9 Antibody Picoband&reg; IF analysis of GLUT9 using anti-GLUT9 antibody (PB9891). <br> GLUT9 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-GLUT9 Antibody (PB9891) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9891-5.jpgAnti-GLUT9/SLC2A9 Antibody Picoband&reg; IF analysis of GLUT9 using anti-GLUT9 antibody (PB9891). <br> GLUT9 was detected in paraffin-embedded section of rat intestinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-GLUT9 Antibody (PB9891) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9891-6.jpgAnti-GLUT9/SLC2A9 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-GLUT9 antibody (PB9891).<br>Overlay histogram showing U937 cells stained with PB9891 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GLUT9 Antibody (PB9891,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smurf1-picoband-trade-antibody-pb9892-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9892-1-WB-anti-smurf1-picoband-antibody.jpgAnti-SMURF1 Antibody Picoband&reg; Western blot analysis of SMURF1 using anti-SMURF1 antibody (PB9892). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF1 antigen affinity purified polyclonal antibody (Catalog # PB9892) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF1 at approximately 86 kDa. The expected band size for SMURF1 is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9892-smurf1-primary-antibodies-ihc-testing-2.jpgAnti-SMURF1 Antibody Picoband&reg; IHC analysis of SMURF1 using anti-SMURF1 antibody (PB9892). <br> SMURF1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMURF1 Antibody (PB9892) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9892-smurf1-primary-antibodies-ihc-testing-3.jpgAnti-SMURF1 Antibody Picoband&reg; IHC analysis of SMURF1 using anti-SMURF1 antibody (PB9892). <br> SMURF1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SMURF1 Antibody (PB9892) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9892-smurf1-primary-antibodies-if-testing-4.jpgAnti-SMURF1 Antibody Picoband&reg; IF analysis of SMURF1 using anti-SMURF1 antibody (PB9892). <br> SMURF1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SMURF1 Antibody (PB9892) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9892-smurf1-primary-antibodies-fcm-testing-5.pngAnti-SMURF1 Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-SMURF1 antibody (PB9892).<br>Overlay histogram showing MCF-7 cells stained with PB9892 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMURF1 Antibody (PB9892,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smyd3-picoband-trade-antibody-pb9893-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9893-1-WB-anti-smyd3-picoband-antibody.jpgAnti-SMYD3 Antibody Picoband&reg; Western blot analysis of SMYD3 using anti-SMYD3 antibody (PB9893). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: COLO320 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMYD3 antigen affinity purified polyclonal antibody (Catalog # PB9893) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMYD3 at approximately 55 kDa. The expected band size for SMYD3 is at 49 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9893-2-IHC-anti-smyd3-picoband-antibody.jpgAnti-SMYD3 Antibody Picoband&reg; IHC analysis of SMYD3 using anti-SMYD3 antibody (PB9893). <br> SMYD3 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SMYD3 Antibody (PB9893) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sptlc1-picoband-trade-antibody-pb9894-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9894-sptlc1-primary-antibodies-wb-testing-1_1.jpgAnti-SPTLC1 Antibody Picoband&reg; Western blot analysis of SPTLC1 using anti-SPTLC1 antibody (PB9894). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hacat whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human SIHA whole cell lysates,<br> Lane 4: human U251 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPTLC1 antigen affinity purified polyclonal antibody (Catalog # PB9894) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPTLC1 at approximately 53 kDa. The expected band size for SPTLC1 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9894-2-IHC-anti-sptlc1-picoband-antibody.jpgAnti-SPTLC1 Antibody Picoband&reg; IHC analysis of SPTLC1 using anti-SPTLC1 antibody (PB9894). <br> SPTLC1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SPTLC1 Antibody (PB9894) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9894-3.jpgAnti-SPTLC1 Antibody Picoband&reg; IF analysis of SPTLC1 using anti-SPTLC1 antibody (PB9894).<br> SPTLC1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SPTLC1 Antibody (PB9894) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9894-4.pngAnti-SPTLC1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-SPTLC1 antibody (PB9894). <br> Overlay histogram showing U20S cells stained with PB9894 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPTLC1 Antibody (PB9894&#44; 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-staufen-picoband-trade-antibody-pb9895-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9895-stau1-primary-antibodies-wb-testing-1.jpgAnti-Staufen/STAU1 Antibody Picoband&reg; Western blot analysis of Staufen/STAU1 using anti-Staufen/STAU1 antibody (PB9895). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human CACO-2 whole cell lysates,<br> Lane 4: human SiHa whole cell lysates,<br> Lane 5: human U20S whole cell lysates,<br> Lane 6: rat PC-12 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Staufen/STAU1 antigen affinity purified polyclonal antibody (Catalog # PB9895) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Staufen/STAU1 at approximately 55 kDa. The expected band size for Staufen/STAU1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9895-stau1-primary-antibodies-if-testing-1.jpgAnti-Staufen/STAU1 Antibody Picoband&reg;IF analysis of Staufen/STAU1 using anti-Staufen/STAU1 antibody (PB9895). <br> Staufen/STAU1 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Staufen/STAU1 Antibody (PB9895) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-stip1-picoband-trade-antibody-pb9896-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9896-stip1-primary-antibodies-wb-testing-1.jpgAnti-STIP1 Antibody Picoband&reg; Western blot analysis of STIP1 using anti-STIP1 antibody (PB9896). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human COLO320 whole cell lysates,<br> Lane 3: human MCF-7 whole cell lysates,<br> Lane 4: rat C6 whole cell lysates,<br> Lane 5: rat RH35 whole cell lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates,<br> Lane 9: mouse HEPA1-6 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIP1 antigen affinity purified polyclonal antibody (Catalog # PB9896) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STIP1 at approximately 63 kDa. The expected band size for STIP1 is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tcf7l1-picoband-trade-antibody-pb9897-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9897-1-WB-anti-tcf7l1-tcf3-picoband-antibody.jpgAnti-TCF7L1 Antibody Picoband&reg; Western blot analysis of TCF7L1 using anti-TCF7L1 antibody (PB9897). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: COLO320 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF7L1 antigen affinity purified polyclonal antibody (Catalog # PB9897) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCF7L1 at approximately 63 kDa. The expected band size for TCF7L1 is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9897-2-IHC-anti-tcf7l1-tcf3-picoband-antibody.jpgAnti-TCF7L1 Antibody Picoband&reg; IHC analysis of TCF7L1 using anti-TCF7L1 antibody (PB9897). <br> TCF7L1 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TCF7L1 Antibody (PB9897) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd134-ox40-picoband-trade-antibody-pb9898-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9898-1-WB-anti-tnfrsf4-ox40-picoband-antibody.jpgAnti-CD134/OX40/TNFRSF4 Antibody Picoband&reg; Western blot analysis of CD134 using anti-CD134 antibody (PB9898). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates&#44; <br> Lane 2: 22RV1 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134 antigen affinity purified polyclonal antibody (Catalog # PB9898) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134 at approximately 50KD. The expected band size for CD134 is at 50KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9898-2-IHC-anti-tnfrsf4-ox40-picoband-antibody.jpgAnti-CD134/OX40/TNFRSF4 Antibody Picoband&reg; IHC analysis of CD134 using anti-CD134 antibody (PB9898).<br> CD134 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD134 Antibody (PB9898) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9898-cd134-primary-antibodies-fc-testing-3.pngAnti-CD134/OX40/TNFRSF4 Antibody Picoband&reg; Flow Cytometry analysis of H-PBMC cells using anti-CD134 antibody (PB9898).<br> Overlay histogram showing H-PBMC cells stained with PB9898 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD134 Antibody (PB9898&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tpp1-picoband-trade-antibody-pb9899-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9899-1-WB-anti-tpp1-tripeptidyl-peptidase-i-picoband-antibody.jpgAnti-TPP1 Antibody Picoband&reg; Western blot analysis of TPP1 using anti-TPP1 antibody (PB9899). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPP1 antigen affinity purified polyclonal antibody (Catalog # PB9899) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPP1 at approximately 39 kDa. The expected band size for TPP1 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9899-2-IHC-anti-tpp1-tripeptidyl-peptidase-i-picoband-antibody.jpgAnti-TPP1 Antibody Picoband&reg; IHC analysis of TPP1 using anti-TPP1 antibody (PB9899). <br> TPP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TPP1 Antibody (PB9899) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trib2-picoband-trade-antibody-pb9900-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9900-1-WB-anti-trib2-trb-2-picoband-antibody.jpgAnti-TRIB2 Antibody Picoband&reg; Western blot analysis of TRIB2 using anti-TRIB2 antibody (PB9900). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIB2 antigen affinity purified polyclonal antibody (Catalog # PB9900) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIB2 at approximately 39 kDa. The expected band size for TRIB2 is at 39 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9900-2-IHC-anti-trib2-trb-2-picoband-antibody.jpgAnti-TRIB2 Antibody Picoband&reg; IHC analysis of TRIB2 using anti-TRIB2 antibody (PB9900). <br> TRIB2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRIB2 Antibody (PB9900) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9900-3-IHC-anti-trib2-trb-2-picoband-antibody.jpgAnti-TRIB2 Antibody Picoband&reg; IHC analysis of TRIB2 using anti-TRIB2 antibody (PB9900). <br> TRIB2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRIB2 Antibody (PB9900) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9900-4-IHC-anti-trib2-trb-2-picoband-antibody.jpgAnti-TRIB2 Antibody Picoband&reg; IHC analysis of TRIB2 using anti-TRIB2 antibody (PB9900). <br> TRIB2 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRIB2 Antibody (PB9900) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpm4-picoband-trade-antibody-pb9902-boster.html2026-03-24T05:05:28+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9902-trpm4-primary-antibodies-wb-testing-1.jpgAnti-TRPM4 Antibody Picoband&reg; Western blot analysis of TRPM4 using anti-TRPM4 antibody (PB9902). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM4 antigen affinity purified polyclonal antibody (Catalog # PB9902) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM4 at approximately 167 kDa. The expected band size for TRPM4 is at 134 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9902-trpm4-primary-antibodies-ihc-testing-2.jpgAnti-TRPM4 Antibody Picoband&reg; IHC analysis of TRPM4 using anti-TRPM4 antibody (PB9902). <br> TRPM4 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRPM4 Antibody (PB9902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9902-trpm4-primary-antibodies-ihc-testing-3.jpgAnti-TRPM4 Antibody Picoband&reg; IHC analysis of TRPM4 using anti-TRPM4 antibody (PB9902). <br> TRPM4 was detected in a paraffin-embedded section of human squamous cell carcinoma of cervix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TRPM4 Antibody (PB9902) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpm5-picoband-trade-antibody-pb9903-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9903-1-WB-anti-trpm5-picoband-antibody.jpgAnti-TRPM5 Antibody Picoband&reg; Western blot analysis of TRPM5 using anti-TRPM5 antibody (PB9903). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SMMC whole cell lysates,<br> Lane 2: 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM5 antigen affinity purified polyclonal antibody (Catalog # PB9903) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM5 at approximately 131 kDa. The expected band size for TRPM5 is at 131 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9903-2-IHC-anti-trpm5-picoband-antibody.jpgAnti-TRPM5 Antibody Picoband&reg; IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903). <br> TRPM5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9903-3-IHC-anti-trpm5-picoband-antibody.jpgAnti-TRPM5 Antibody Picoband&reg; IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903). <br> TRPM5 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9903-4-IHC-anti-trpm5-picoband-antibody.jpgAnti-TRPM5 Antibody Picoband&reg; IHC analysis of TRPM5 using anti-TRPM5 antibody (PB9903). <br> TRPM5 was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-TRPM5 Antibody (PB9903) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-uba1-picoband-trade-antibody-pb9904-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9904-1-WB-anti-uba1-ube1-picoband-antibody.jpgAnti-E1 Ubiquitin Activating Enzyme/UBA1 Antibody Picoband&reg; Western blot analysis of UBA1 using anti-UBA1 antibody (PB9904). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: mouse liver tissue lysates.<br> Lane 3: mouse testis tissue lysates.<br> Lane 4: HELA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA1 antigen affinity purified polyclonal antibody (Catalog # PB9904) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA1 at approximately 117 kDa. The expected band size for UBA1 is at 118 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-uhrf1-picoband-trade-antibody-pb9905-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9905-uhrf1-primary-antibodies-wb-testing-1.jpgAnti-UHRF1 Antibody Picoband&reg; Western blot analysis of UHRF1 using anti-UHRF1 antibody (PB9905). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human T47D whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: human MDA-MB-453 whole cell lysates,<br> Lane 6: human Raji whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UHRF1 antigen affinity purified polyclonal antibody (Catalog # PB9905) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UHRF1 at approximately 100 kDa. The expected band size for UHRF1 is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9905-2-IHC-anti-uhrf1-picoband-antibody.jpgAnti-UHRF1 Antibody Picoband&reg; IHC analysis of UHRF1 using anti-UHRF1 antibody (PB9905). <br> UHRF1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-UHRF1 Antibody (PB9905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9905-3.pngAnti-UHRF1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-UHRF1 antibody (PB9905). <br> Overlay histogram showing U20S cells stained with PB9905 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UHRF1 Antibody (PB9905&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9905-4.pngAnti-UHRF1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-UHRF1 antibody (PB9905). <br> Overlay histogram showing A431 cells stained with PB9905 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UHRF1 Antibody (PB9905&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9905-uhrf1-primary-antibodies-if-testing-5_1.jpgAnti-UHRF1 Antibody Picoband&reg; IF analysis of UHRF1 using anti-UHRF1 antibody (PB9905). <br> UHRF1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-UHRF1 Antibody (PB9905) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nirf-picoband-trade-antibody-pb9906-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9906-1-WB-anti-nirf-picoband-antibody.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg; Western blot analysis of NIRF using anti-NIRF antibody (PB9906). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates,<br> Lane 2: K562 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIRF antigen affinity purified polyclonal antibody (Catalog # PB9906) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NIRF at approximately 90 kDa. The expected band size for NIRF is at 90 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9906-2-IHC-anti-nirf-picoband-antibody.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg; IHC analysis of NIRF using anti-NIRF antibody (PB9906). <br> NIRF was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NIRF Antibody (PB9906) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9906-ott-10-5863fig1.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg;The expression of UHRF2 in human cancers and ICC patients. Notes: ( A ) Microarray data analysis of UHRF2 mRNA in gastric cancer, colon cancer, and hepatocellular carcinoma from the publicly available Oncomine database. ( B ) UHRF2 protein expression in randomly selected ICC tissues and their matched peritumor tissues by Western blot. ( C ) Relative expression of UHRF2 among tumor and peritumor tissues using qRT-PCR. ( D ) Positive UHRF2 was observed primarily in the cytoplasm. Representative UHRF2 and H<E staining are displayed. Scale bar =50 μm, magnification: 200×. ( E ) The density analysis revealed statistical significance of UHRF2 level of 100 cases of patients in TMA samples. * P <;0.05, *** P <;0.001. Abbreviations: H<E, hematoxylin and eosin; ICC, intrahepatic cholangiocarcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; TMA, tissue microarray; UHRF2, ubiquitin-like with PHD and ring finger domains 2; IHC, immunohistochemistry.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5729825/&orig_host=www.ncbi.nlm.nih.gov'>29270024</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9906-ott-10-5863fig2.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg;UHRF2 expression resulted in ICC cell proliferation, invasion, migration, and antiapoptosis. Notes: ( A ) UHRF2 expression was interfered by siRNA and confirmed by Western blot and qRT-PCR. GAPDH was used as internal control. ( B ) Cell counting kit-8 assay was used to assess the ability of cell proliferation. ( C ) Transwell assay was used to show the invasion of ICC cells with different UHFR2 expression in 48 hours. Scale bar =100 μm. ( D ) Wound healing assays showed that inhibition of UHRF2 decreased wound healing compared with control cells. Scale bar =100 μm. ( E ) FCM results indicated that anti-UHRF2 caused acceleration of cell apoptosis. The results are mean ± SD of triplicated independent experiments. * P <;0.05, ** P <;0.01, *** P <;0.001. Abbreviations: FCM, flow cytometry; FITC, fluorescein isothiocyanate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICC, intrahepatic cholangiocarcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; UHRF2, ubiquitin-like with PHD and ring finger domains 2.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5729825/&orig_host=www.ncbi.nlm.nih.gov'>29270024</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9906-ott-10-5863fig2a.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg;UHRF2 expression resulted in ICC cell proliferation, invasion, migration, and antiapoptosis. Notes: ( A ) UHRF2 expression was interfered by siRNA and confirmed by Western blot and qRT-PCR. GAPDH was used as internal control. ( B ) Cell counting kit-8 assay was used to assess the ability of cell proliferation. ( C ) Transwell assay was used to show the invasion of ICC cells with different UHFR2 expression in 48 hours. Scale bar =100 μm. ( D ) Wound healing assays showed that inhibition of UHRF2 decreased wound healing compared with control cells. Scale bar =100 μm. ( E ) FCM results indicated that anti-UHRF2 caused acceleration of cell apoptosis. The results are mean ± SD of triplicated independent experiments. * P <;0.05, ** P <;0.01, *** P <;0.001. Abbreviations: FCM, flow cytometry; FITC, fluorescein isothiocyanate; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICC, intrahepatic cholangiocarcinoma; qRT-PCR, quantitative real-time polymerase chain reaction; UHRF2, ubiquitin-like with PHD and ring finger domains 2.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5729825/&orig_host=www.ncbi.nlm.nih.gov'>29270024</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9906-ott-10-5863fig3.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg;UHRF2 was negatively associated with E-cadherin expression in ICC. Notes: ( A ) High expression of UHRF2 and low expression of E-cadherin, and low expression of UHRF2 and high expression of E-cadherin are shown. Scale bar: 100, 200 μm. ( B ) The relationship between UHRF2 and E-cadherin in ICC patients; the protein expression of UHRF2 was significantly negatively correlated with E-cadherin expression. ( C ) UHRF2 and E-cadherin protein were detected by Western blot analysis. ( D ) Representative immunofluorescent images of UHRF2 and E-cadherin in ICC cell lines are shown. Scale bars =100 μm. * P <;0.05, ** P <;0.01, *** P <;0.001. Abbreviations: H<E, hematoxylin and eosin; ICC, intrahepatic cholangiocarcinoma; UHRF2, ubiquitin-like with PHD and ring finger domains 2.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5729825/&orig_host=www.ncbi.nlm.nih.gov'>29270024</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9906-ott-10-5863fig4.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg;Prognostic significance was assessed by log-rank tests and Kaplan–Meier analysis. Notes: ( A – D ) Representative graphs of H<E and immunohistochemical staining for UHRF2 in ICC samples: ( A ) absence; ( B ) low; ( C ) moderate; ( D ) strong. Scale bar: 100, 200 μm. ( E and F ). The effects of UHRF2 expression on prognosis in patients with ICC are illustrated. Abbreviations: H<E, hematoxylin and eosin; ICC, intrahepatic cholangiocarcinoma; UHRF2, ubiquitin-like with PHD and ring finger domains 2.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC5729825/&orig_host=www.ncbi.nlm.nih.gov'>29270024</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9906-3-IHC-anti-nirf-picoband-antibody.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg; IHC analysis of NIRF using anti-NIRF antibody (PB9906). <br> NIRF was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NIRF Antibody (PB9906) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9906-4-IHC-anti-nirf-picoband-antibody.jpgAnti-NIRF/UHRF2 Antibody Picoband&reg; IHC analysis of NIRF using anti-NIRF antibody (PB9906). <br> NIRF was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-NIRF Antibody (PB9906) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vrk1-picoband-trade-antibody-pb9907-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9907-1-WB-anti-vrk1-picoband-antibody.jpgAnti-VRK1 Antibody Picoband&reg; Western blot analysis of VRK1 using anti-VRK1 antibody (PB9907). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: JURKAT whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRK1 antigen affinity purified polyclonal antibody (Catalog # PB9907) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRK1 at approximately 55 kDa. The expected band size for VRK1 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xrcc4-picoband-trade-antibody-pb9908-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9908-1-WB-anti-xrcc4-picoband-antibody.jpgAnti-XRCC4 Antibody Picoband&reg; Western blot analysis of XRCC4 using anti-XRCC4 antibody (PB9908). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates,<br> Lane 2: A431 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XRCC4 antigen affinity purified polyclonal antibody (Catalog # PB9908) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XRCC4 at approximately 55 kDa. The expected band size for XRCC4 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9908-2-IHC-anti-xrcc4-picoband-antibody.jpgAnti-XRCC4 Antibody Picoband&reg; IHC analysis of XRCC4 using anti-XRCC4 antibody (PB9908). <br> XRCC4 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-XRCC4 Antibody (PB9908) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-yy1-picoband-trade-antibody-pb9909-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909-yy1-primary-antibodies-wb-testing-1.jpgAnti-YY1 Antibody Picoband&reg; Western blot analysis of YY1 using anti-YY1 antibody (PB9909). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse thymus tissue lysates,<br> Lane 2: mouse spleen tissue lysates,<br> Lane 3: rat thymus tissue lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse RAW264.7 whole cell lysates,<br> Lane 6: human PC-3 whole cell lysates,<br> Lane 7: human SW620 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YY1 antigen affinity purified polyclonal antibody (Catalog # PB9909) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909_41467_2025_61560_fig1_html.pngAnti-YY1 Antibody Picoband&reg;YY1 + macrophages accumulate in hypoxic tumor tissues. a Schematic diagram showing the process of imaging mass cytometry (IMC) using prostate tissue. IMC of the indicated markers in prostate cancer (PCa) patient tumors ( b ) and para-cancerous tissues ( c ). Pathchs were defined as containing at least 10 cells with a maximum distance of 15 μm between cells involved. Adjacent regions were merged together for downstream analysis. The expression of the marker was normalized using z -score, and a threshold of 0.5 was selected. Dot plots showing the ratio of YY1 + or YY1 − macrophages and the relative YY1 expression of macrophages in HIF-1α + or HIF-1α − patches. The representative plot and analysis are based on 46 tumor samples and 26 para-cancerous tissues. Dot plots present the mean values ± SD, and the p values are calculated using a two-tailed t -test. The p values of left, middle, and right plots of ( b ) are 0.002, 0.160, and 0.009, respectively; The p values of left, middle, and right plots of ( c ) are 0.759, 0.798, and 0.820, respectively. * P < 0.05, ns P > 0.05. Source data are provided as a file. Mø macrophage. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-61560-0'>40623999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909_41467_2025_61560_fig2_html.pngAnti-YY1 Antibody Picoband&reg;Hypoxia enhances YY1 phase separation by inducing YY1 tyrosine phosphorylation in macrophages. a Immunofluorescence staining in THP-1 cells subjected to 1% O 2 and/or 1,6-hexanediol. The p values were calculated using both two-tailed t -test ( P = 0.017 and P < 0.001) and one-way ANOVA followed by Tukey test ( P < 0.001). b The localization of YY1 was observed by immunofluorescence after hypoxia with 1% O 2 or reoxygenation with 21% O 2 . c Western blot of HIF - 1α and YY1 in THP-1 cells under 1% O 2 hypoxia. d qRT‒PCR showing the relative RNA expression of YY1 in THP-1 cells under 1% O 2 hypoxia. e Western blot analysis using an anti-phosphorylated YY1 antibody to detect YY1-immunoprecipitated proteins. f Immunofluorescence staining in THP-1 cells treated with Na 3 VO 4 or control solution under normal oxygen conditions ( P < 0.001). g Western blot analysis using a tyrosine phosphorylation antibody to detect YY1-immunoprecipitated protein. The bacterial alkaline phosphatase was used as a negative control. h Immunofluorescence staining in THP-1 cells treated with SU6656 or control solution under 1% O 2 hypoxia ( P < 0.001). i Western blot analysis using a tyrosine phosphorylation antibody to detect EGFP immunoprecipitated from YY1 full-length (YY1-FL), YY1 mutation 1 (YY1-mut1, including amino acid sites 8 and 383), and YY1 mutation 2 (YY1-mut2, including amino acids 145, 185, 251, and 254)-transfected THP-1 cells. j Immunofluorescence of YY1-FL-, YY1-mut1- and YY1-mut2-transfected THP-1 cells. k The left volcano plot illustrates the differentially expressed genes in the indicated RNA-seq (two-tailed t-test and adjusted with Benjamini and Hochberg method). The right panel shows the overlap indicated to identify Lyn. l , m Co-IP and immunofluorescence of YY1 and Lyn from 1% O 2 hypoxia- or normoxia-induced THP-1 cells. n Immunofluorescence of the indicated markers in THP-1 cells under 1% O 2 hypoxia ( P < 0.001). All the immunofluorescence data were analyzed, and twenty cells randomly selected from three repeated groups were counted. Scale bar, 5 μm. Bar graphs ( a , b , d , f , h , n ) present mean values ± SD, and the p values are calculated using a two-tailed t -test. * P < 0.05. Source data are provided as a file. 1,6-Hex 1,6-hexanediol, BAP bacterial alkaline phosphatase. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-61560-0'>40623999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909_41467_2025_61560_fig3_html.pngAnti-YY1 Antibody Picoband&reg;YY1 stabilizes HIF-1α by promoting its SUMOylation and inhibiting its ubiquitination. a Venn diagram depicting the overlap between two datasets: (1) RNA-seq analysis comparing oe-YY1 and oe-NC THP-1 cells, and (2) a previously reported RNA-seq dataset from hypoxia-induced macrophages (GSE16099). b GSEA analysis of the HIF-1A targets DN set, comparing RNA-seq data from oe-YY1 THP-1 cells to nc-YY1 THP-1 cells. NES and p value are indicated (phenotype permutation test). c , d Gene ontology analysis of enriched signaling pathways in oe-YY1 or hypoxia-induced THP-1 cells when compared to control cells (hypergeometric test and corrected with Benjamini & Hochberg adjustment). e Western blot showing the expression of HIF-1α and YY1 in 1% O 2 hypoxia-induced THP-1 cells transfected with siYY1 or the normal control. Samples calculated are from the same experiment, and the blots were processed in parallel ( n = 3 independent experiments, P < 0.001, P < 0.001, respectively). f qRT‒PCR showing the expression of YY1 and HIF-1α in THP-1 cells ( n = 3 independent experiments, P < 0.001, P = 0.220, respectively). g Western blot of HIF-1α expression in 1% O 2 hypoxia-induced THP-1 cells treated with cycloheximide (n = 3 independent experiments). The lower panel presents mean values ± SD at each time point (linear regression between the two datasets using two-tailed t -test, P < 0.001). h Western blot of HIF-1α expression in THP - 1 cells treated with MG132 and/or 1% O 2 hypoxia. i , j Immunoprecipitation of HIF-1α followed with western blot of ubiquitin or SUMO2/3 in 1% O 2 hypoxia-induced THP-1 cells. Bar graphs ( e , f ) present mean values ± SD, and the p values are calculated using a two-tailed t -test. * P < 0.05, ns P > 0.05. Source data are provided as a file. NES normalized enrichment scores, FDR false discovery rate, CHX cycloheximide. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-61560-0'>40623999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909_41467_2025_61560_fig4_html.pngAnti-YY1 Antibody Picoband&reg;YY1 promotes the stability of HIF-1α by binding to NUSAP1. a Mass spectrometry of proteins immunoprecipitated with YY1 and IgG under CoCl₂ treatment conditions. b In the co-IP experiment using 1% O 2 hypoxia-induced THP-1 cells, the expression levels of YY1, NUSAP1, and HIF-1α proteins were detected in the proteins immunoprecipitated by the YY1 or IgG antibody. c Western blot of HIF-1α in THP-1 cells treated with MG132. d , e Western blot of ubiquitin or SUMO2/3 following HIF-1α IP in hypoxia-induced THP-1 cells. f Diagram of the SAP domain and N/C-terminus of NUSAP1. g , h SUMOylation assays in hypoxia-induced THP-1 cells under the indicated conditions. i CO-IP assays in hypoxia-induced THP-1 cells showing the interactions between Flag-tagged NUSAP1 segments and EGFP-tagged YY1. j CO-IP assays in hypoxia-induced THP-1 cells showing the interactions between EGFP-tagged YY1 segments and Flag-tagged NUSAP1. FL, NTD, and CTD represent the full-length, N-terminal domain, and C-terminal domain of YY1, respectively. k , l Co-IP assays showing the interactions of the indicated HA-YY1 or Flag-NUSAP1 mutant. m , n Co-IP assays showing the interactions of truncated HIF-1α and NUSAP1 proteins in hypoxia-induced THP-1 cells. o Immunofluorescence assays showing the colocalization of the indicated markers in THP-1 cells treated with hypoxia or normoxia. The dye intensity alongside the white lines was calculated and plotted in the right panels. Scale bar, 2 μm. p Schematic diagram of the NUSAP1 IDR. q Fluorescence images of m-cherry showing droplet formation in the medium under different concentrations of NUSAP1-m-cherry protein. Scale bar, 5 μm. r Fluorescence images of EGFP and m-cherry showing the physical colocalization of NUSAP1-m-cherry with the YY1-IDR-EGFP or YY1-non-IDR-EGFP in the medium. Scale bar, 5 μm. Source data are provided as a file. NTD N-terminal domain, CTD C-terminal domain, FL full-length. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-61560-0'>40623999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909_41467_2025_61560_fig6_html.pngAnti-YY1 Antibody Picoband&reg;A small molecule inhibitor targeting the YY1–NUSAP1–HIF-1α interaction suppressed PCa progression. a Schematic diagram showing that tenapanor inhibits the binding of the YY1–NUSAP1–HIF-1α nexus. b , c Co-IP of NUSAP1 showed that its interactions with YY1 and HIF-1α were suppressed by tenapanor. The protein intensity of YY1 and HIF-1α pulled down from NUSAP1 was analyzed with ImageJ and standardized to that of the input samples from the same experiment. The blots were processed in parallel ( n = 3 independent experiments, P < 0.001 and P < 0.001). * P < 0.05. d , e Schematic diagram and tumor growth curve of the effects of TEN-M2pep and control medium on subcutaneous tumorigenesis in mice. Each experimental group consisted of five male C57BL/6 mice, all aged 6–8 weeks. The right panels present the mean values ± SD at each time point ( n = 5 independent experiments, one-way ANOVA followed by Tukey test based on data in the last time point). f , g GO analysis based on RNA-sequencing data of CD45-sorted cells from TEN-M2pep-treated subcutaneous tumors showing enriched immune response-related pathways and representative upregulated genes (two-tailed t -test). h , i Immunohistochemical staining and flow cytometry of subcutaneous tumors showing the infiltration of CD8 + T cells in the indicated groups ( n = 3 independent experiments, P = 0.019 and P = 0.002). Bar graphs ( c , i ) present the mean values ± SD, and the p values are calculated using a two-tailed t -test. * P < 0.05. Source data are provided as a file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-61560-0'>40623999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909_41467_2025_61560_fig7_html.pngAnti-YY1 Antibody Picoband&reg;Construction and application of YY1-DbTACs@jetPEI and transgenic mice. a Schematic diagram illustrating the mechanism of YY1-DbTACs-mediated degradation of YY1. b Western blot in THP-1 cells treated with YY1-DbTACs. c Western blot of ubiquitin following HIF-1α IP in hypoxia-induced THP-1 cells treated with YY1-DbTACs. d Experimental flow chart of subcutaneous tumorigenesis in mice using M2pep-YY1/NC-DbTACs@jetPEI. Each experimental group consisted of five male C57BL/6 mice (6–8 weeks). e Tumor volume changes over 21 days following subcutaneous tumor implantation. The lower panel presents the mean values ± SD at each time point. The p values were calculated based on the last time point between two datasets using a two-tailed t -test ( n = 5 independent experiments, P < 0.001). * P < 0.05. f Schematic diagram illustrating the mechanism of YY1-DcTACs and its tetrahedral structure. g Confocal microscopy images showing the responsiveness of YY1-DcTACs-FAM/BHQ1 in the indicated cell lines. h Changes in tumor volume within 14 days after injection of YY1/NC-DcTACs via the tail vein in a mouse subcutaneous tumor model. The T lymphocytes were sorted by flow cytometry before testing changes in the ratio of CD3 + CD8 + T cells. The lower panel presents the mean values ± SD at each time point. The p values were calculated based on the last time point between the two datasets (two-tailed t -test, P < 0.001). * P < 0.05. i Schematic diagram illustrating the construction of YY1 transgenic mice with myeloid conditional knockout and the establishment of a subcutaneous tumorigenesis model. j Subcutaneous tumor size in mice after 21 days of tumor formation ( n = 5 independent experiments, P < 0.001). Five wild-type male C57BL/6J mice and five C57BL/6J-YY1 em1Cflox male mice were used in the experiment (6–8 weeks old). k T lymphocytes were sorted by flow cytometry in the CD45 - gated immune cells after grinding the subcutaneous tumors of transgenic mice into a cell suspension, and changes in the ratio of CD3 + CD8 + T cells were observed ( n = 5 independent experiments, P < 0.001). Bar graphs ( h , j , k ) present the mean values ± SD, and the p values are calculated using a two-tailed t -test. * P < 0.05. Source data are provided as a file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-025-61560-0'>40623999</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909-2.jpgAnti-YY1 Antibody Picoband&reg; IF analysis of YY1 using anti-YY1 antibody (PB9909).<br> YY1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-YY1 Antibody (PB9909) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9909-3.jpgAnti-YY1 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-YY1 antibody (PB9909). <br> Overlay histogram showing THP-1 cells stained with PB9909 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YY1 Antibody (PB9909&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zbtb7a-picoband-trade-antibody-pb9910-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9910-zbtb7a-primary-antibodies-wb-testing-1.jpgAnti-ZBTB7A Antibody Picoband&reg; Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified polyclonal antibody (Catalog # PB9910) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 72 kDa. The expected band size for ZBTB7A is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9910-2-IHC-anti-zbtb7a-pokemon-picoband-antibody.jpgAnti-ZBTB7A Antibody Picoband&reg; IHC analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910). <br> ZBTB7A was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZBTB7A Antibody (PB9910) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9910-zbtb7a-primary-antibodies-if-testing-3.jpgAnti-ZBTB7A Antibody Picoband&reg; IF analysis of ZBTB7A using anti-ZBTB7A antibody (PB9910). <br> ZBTB7A was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ZBTB7A Antibody (PB9910) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9910-zbtb7a-primary-antibodies-fcm-testing-4.pngAnti-ZBTB7A Antibody Picoband&reg; Flow Cytometry analysis of MCF-7 cells using anti-ZBTB7A antibody (PB9910). <br>Overlay histogram showing MCF-7 cells stained with PB9910 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZBTB7A Antibody (PB9910, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zbtb7a-picoband-trade-antibody-pb9911-boster.html2026-03-29T05:00:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9911-1-WB-anti-zbtb7a-pokemon-picoband-antibody.jpgAnti-ZBTB7A Antibody Picoband&reg; Western blot analysis of ZBTB7A using anti-ZBTB7A antibody (PB9911). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse kidney tissue lysates,<br> Lane 2: NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZBTB7A antigen affinity purified polyclonal antibody (Catalog # PB9911) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZBTB7A at approximately 75 kDa. The expected band size for ZBTB7A is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9911-2-IHC-anti-zbtb7a-pokemon-picoband-antibody.jpgAnti-ZBTB7A Antibody Picoband&reg; IHC analysis of ZBTB7A using anti-ZBTB7A antibody (PB9911). <br> ZBTB7A was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZBTB7A Antibody (PB9911) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9911-3-IHC-anti-zbtb7a-pokemon-picoband-antibody.jpgAnti-ZBTB7A Antibody Picoband&reg; IHC analysis of ZBTB7A using anti-ZBTB7A antibody (PB9911). <br> ZBTB7A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZBTB7A Antibody (PB9911) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zp2-picoband-trade-antibody-pb9912-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9912-1-WB-anti-zp2-picoband-antibody.jpgAnti-ZP2 Antibody Picoband&reg; Western blot analysis of ZP2 using anti-ZP2 antibody (PB9912). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: HEPG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP2 antigen affinity purified polyclonal antibody (Catalog # PB9912) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP2 at approximately 82 kDa. The expected band size for ZP2 is at 82 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9912-2-IHC-anti-zp2-picoband-antibody.jpgAnti-ZP2 Antibody Picoband&reg; IHC analysis of ZP2 using anti-ZP2 antibody (PB9912). <br> ZP2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZP2 Antibody (PB9912) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9912-zp2-primary-antibodies-ihc-testing-3.jpgAnti-ZP2 Antibody Picoband&reg; IHC analysis of ZP2 using anti-ZP2 antibody (PB9912). <br> ZP2 was detected in frozen section of human placenta tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ZP2 Antibody (PB9912) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smurf-2-antibody-rp1102-boster.html2026-03-24T05:05:29+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1102-1-WB-anti-smurf-2-antibody.jpgAnti-SMURF 2/SMURF2 Antibody Picoband&reg; Western blot analysis of SMURF2 using anti-SMURF2 antibody (RP1102). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: SMMC whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # RP1102) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1102-2.jpgAnti-SMURF 2/SMURF2 Antibody Picoband&reg; Western blot analysis of SMURF2 using anti-SMURF2 antibody (RP1102). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates&#44; <br> Lane 2: rat pancreas tissue lysates&#44; <br> Lane 3: rat stomach testis lysates&#44; <br> Lane 4: mouse testis tissue lysates. <br> Lane 5: mouse pancreas tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF2 antigen affinity purified polyclonal antibody (Catalog # RP1102) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF2 at approximately 86KD. The expected band size for SMURF2 is at 86KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd153-antibody-rp1103-boster.html2026-03-25T05:22:30+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1103-1-IHC-anti-cd30l-antibody.jpgAnti-CD153/TNFSF8 AntibodyCD153 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-CD153 Antigen Affinity purified polyclonal antibody (Catalog # RP1103) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1103-cd153-primary-antibodies-fc-testing-2.pngAnti-CD153/TNFSF8 Antibody2. Flow Cytometry analysis of U937 cells using anti-CD153 antibody (RP1103).<br>Overlay histogram showing U937 cells stained with RP1103 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD153 Antibody (RP1103&#44;1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/picokine-elisa-kits/mouse-bmp-4-picokine-trade-elisa-kit-ek0316-boster.html2026-03-24T05:05:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0316_1.pngMouse BMP-4 ELISA Kit PicoKine®Mouse BMP-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-cd23-fcer2-picokine-trade-elisa-kit-ek0924-boster.html2026-03-24T05:05:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0924_1.pngMouse CD23/FCER2 ELISA Kit PicoKine®Mouse CD23/FCER2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-caspase-3-picokine-trade-elisa-kit-ek1425-boster.html2026-03-24T05:05:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1425_1.pngHuman Caspase 3/CASP3 ELISA Kit PicoKine®Human Caspase 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-caspase-8-picokine-trade-elisa-kit-ek1426-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1426_1.pngHuman Caspase 8/CASP8 ELISA Kit PicoKine®Human Caspase 8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl19-mip-3-beta-picokine-trade-elisa-kit-ek0457-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0457_1.pngMouse CCL19/MIP-3 beta ELISA Kit PicoKine®Mouse CCL19 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-agrp-agouti-related-picokine-trade-elisa-kit-ek1427-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1427.jpgHuman AGRP/ART/Agouti-related protein ELISA Kit PicoKine®Human AGRP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-relaxin-2-picokine-trade-elisa-kit-ek1428-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1428_1.pngHuman Relaxin 2 ELISA Kit PicoKine®Human Relaxin 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-e-cadherin-picokine-trade-elisa-kit-ek1434-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1434_1.pngRat E-Cadherin/CDH1/Cadherin-1 ELISA Kit PicoKine®Rat E-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ccl28-picokine-trade-elisa-kit-ek0690-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0690_1.pngHuman CCL28 ELISA Kit PicoKine®Human CCL28 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cxcl4-pf4-picokine-trade-elisa-kit-ek0726-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0726_2.pngHuman CXCL4/PF4 ELISA Kit PicoKine®Human CXCL4/PF4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-egfr-picokine-trade-elisa-kit-ek1433-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1433_1.pngMouse EGFR ELISA Kit PicoKine®Mouse EGFR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-dkk1-picokine-trade-elisa-kit-ek1432-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1432.jpgRat DKK-1 ELISA Kit PicoKine®Rat DKK1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-eotaxin-picokine-trade-elisa-kit-ek1435-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1435_2.pngRat Eotaxin ELISA Kit PicoKine®Rat Eotaxin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-bmp-7-picokine-trade-elisa-kit-ek1443-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1443_1.pngMouse BMP-7 ELISA Kit PicoKine®Mouse BMP-7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-bmp-7-picokine-trade-elisa-kit-ek1444-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1444_1.pngRat BMP-7 ELISA Kit PicoKine®Rat BMP-7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ids-picokine-trade-elisa-kit-ek1452-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1452_1.pngHuman IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®Human IDS PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ids-picokine-trade-elisa-kit-ek1453-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1453_2.pngMouse IDS/Iduronate 2 Sulfatase ELISA Kit PicoKine®Mouse IDS PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/gfp-picokine-trade-elisa-kit-ek1440-boster.html2026-04-03T05:00:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1440_1.pngGFP/Green fluorescent protein ELISA Kit PicoKine®GFP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-elastase-picokine-trade-elisa-kit-ek1445-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1445_2.pngMouse Neutrophil Elastase/ELA2/ELANE ELISA Kit PicoKine®Mouse Elastase PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ada-picokine-trade-elisa-kit-ek1446-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1446.pngHuman ADA/Adenosine Deaminase ELISA Kit PicoKine®Human ADA PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pd-l1-b7-h1-picokine-trade-elisa-kit-ek1449-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1449_1.pngHuman PD-L1/B7-H1 ELISA Kit PicoKine®Human PD-L1/B7-H1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-pd-l1-b7-h1-picokine-trade-elisa-kit-ek1450-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1450_4.pngMouse PD-L1/B7-H1 ELISA Kit PicoKine®Mouse PD-L1/B7-H1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-apoe-picokine-trade-elisa-kit-ek1455-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1455.jpgHuman APOE/Apolipoprotein E ELISA Kit PicoKine®Human APOE PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-apoa1-picokine-trade-elisa-kit-ek1456-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1456.jpgHuman APOA1/Apolipoprotein A-I ELISA Kit PicoKine®Human APOA1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tryptase-tpsab1-b2-picokine-trade-elisa-kit-ek0898-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0898.jpgHuman Tryptase/TPSAB1,B2 ELISA Kit PicoKine®Human Tryptase/TPSAB1,B2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abl2-picoband-trade-antibody-pb9913-boster.html2026-03-24T05:05:37+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9913-abl2-primary-antibodies-wb-testing-1.jpgAnti-ABL2 Antibody Picoband&reg; Western blot analysis of ABL2 using anti-ABL2 antibody (PB9913). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: rat PC-12 whole cell lysates, <br> Lane 3: mouse brain tissue lysates, <br> Lane 4: mouse NIH/3T3 whole cell lysates, <br> Lane 5: huamn K562 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABL2 antigen affinity purified polyclonal antibody (Catalog # PB9913) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABL2 at approximately 140 kDa. The expected band size for ABL2 is at 128 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9913-abl2-primary-antibodies-ihc-testing-2.jpgAnti-ABL2 Antibody Picoband&reg; IHC analysis of ABL2 using anti-ABL2 antibody (PB9913). <br> ABL2 was detected in a paraffin-embedded section of human gallbladder carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABL2 Antibody (PB9913) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9913-abl2-primary-antibodies-ihc-testing-3.jpgAnti-ABL2 Antibody Picoband&reg; IHC analysis of ABL2 using anti-ABL2 antibody (PB9913). <br> ABL2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABL2 Antibody (PB9913) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-afg3l2-picoband-trade-antibody-pb9915-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9915-afg3l2-primary-antibodies-wb-testing-1.jpgAnti-AFG3L2 Antibody Picoband&reg; Western blot analysis of AFG3L2 using anti-AFG3L2 antibody (PB9915). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human MDA-MB-453 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat liver tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFG3L2 antigen affinity purified polyclonal antibody (Catalog # PB9915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AFG3L2 at approximately 89 kDa. The expected band size for AFG3L2 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9915-2.jpgAnti-AFG3L2 Antibody Picoband&reg; IF analysis of AFG3L2 using anti-AFG3L2 antibody (PB9915). <br> AFG3L2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AFG3L2 Antibody (PB9915) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9915-afg3l2-primary-antibodies-ip-testing-1.jpgAnti-AFG3L2 Antibody Picoband&reg;Immunoprecipitating (IP) AFG3L2 in MCF-7 whole cell lysate.<br> Western blot analysis of AFG3L2 using anti-AFG3L2 antibody (PB9915); <br> Lane 1: MCF-7 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-AFG3L2 antibody in MCF-7 whole cell lysate;<br> Lane 3: anti-AFG3L2 antibody (2μg) + MCF-7 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AFG3L2 antigen affinity purified polyclonal antibody (PB9915) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for AFG3L2 at approximately 89 kDa. The expected band size for AFG3L2 is at 89 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9915-3.jpgAnti-AFG3L2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-AFG3L2 antibody (PB9915).<br>Overlay histogram showing A431 cells stained with PB9915 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AFG3L2 Antibody (PB9915,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apoa1-picoband-trade-antibody-pb9916-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9916-1-WB-anti-apoa1-apoa-i-picoband-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; Western blot analysis of APOA1 using anti-APOA1 antibody (PB9916). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOA1 antigen affinity purified polyclonal antibody (Catalog # PB9916) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOA1 at approximately 26 kDa. The expected band size for APOA1 is at 31 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9916-2-IHC-anti-apoa1-apoa-i-picoband-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; IHC analysis of APOA1 using anti-APOA1 antibody (PB9916). <br> APOA1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOA1 Antibody (PB9916) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9916-41598_2021_91521_fig5_html.pngAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg;Comparison of the re-probed ability of PVDF membrane and NC membrane. The pooled sera proteins (3.0, 1.5, 0.7, 0.3 and 0.1 μg) were separated by 8% SDS-PAGE, and transferred to PVDF membranes (up) and NC membrane (down), respectively. ( a ) Staining with AAL and then re-probed with PHA-E; ( b ) staining with ApoA1 and then re-probing with IgG; ( c ) Staining with PHA-E and then re-probing with A2M; ( d ) staining with A2M and then re-probing with PHA-E. Band intensities were statistically analyzed (n = 3 individual experiments) and compared using Image Lab software (Bio-Rad Laboratories) and GraphPad Prism version 6. Pink bar, PVDF membrane; Blue bar, NC membrane. Band intensities were analyzed *Significantly different p < 0.05, ** p < 0.01. N.S., not significant. All values are means ± S.E. (error bars). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-021-91521-8'>34103620</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9916-3-IHC-anti-apoa1-apoa-i-picoband-antibody.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; IHC analysis of APOA1 using anti-APOA1 antibody (PB9916). <br> APOA1 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-APOA1 Antibody (PB9916) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9916-apoa1-primary-antibodies-fcm-testing-4.jpgAnti-Apolipoprotein A I/APOA1 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-APOA1 antibody (PB9916). <br> Overlay histogram showing HepG2 cells stained with PB9916 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APOA1 Antibody (PB9916, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcl-x-picoband-trade-antibody-pb9917-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9917-1-WB-anti-bcl-x-picoband-antibody.jpgAnti-Bcl-X/BCL2L1 Antibody Picoband&reg; Western blot analysis of Bcl-X using anti-Bcl-X antibody (PB9917). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-X antigen affinity purified polyclonal antibody (Catalog # PB9917) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl-X at approximately 29 KD&#44; 60KD. The expected band size for Bcl-X is at 26KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9917-bcl-x-primary-antibodies-fc-testing-2.jpgAnti-Bcl-X/BCL2L1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-Bcl-X antibody (PB9917). <br>Overlay histogram showing PC-3 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X Antibody (PB9917&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9917-bcl-x-primary-antibodies-fc-testing-3.jpgAnti-Bcl-X/BCL2L1 Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-Bcl-X antibody (PB9917). <br>Overlay histogram showing A549 cells stained with PB9917 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcl-X Antibody (PB9917&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-osteocalcin-picoband-trade-antibody-pb9918-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9918-1-IHC-anti-osteocalcin-picoband-antibody.jpgAnti-Osteocalcin/BGLAP Antibody IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9918). <br> Osteocalcin was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Osteocalcin Antibody (PB9918) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-osteocalcin-picoband-trade-antibody-pb9919-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9919-1-IHC-anti-osteocalcin-picoband-antibody.jpgAnti-Osteocalcin/BGLAP Antibody IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9919).<br> Osteocalcin was detected in paraffin-embedded section of mouse tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Osteocalcin Antibody (PB9919) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-osteocalcin-picoband-trade-antibody-pb9920-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9920-1-IHC-anti-osteocalcin-picoband-antibody.jpgAnti-Osteocalcin/BGLAP Antibody IHC analysis of Osteocalcin using anti-Osteocalcin antibody (PB9920).<br> Osteocalcin was detected in paraffin-embedded section of rat tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Osteocalcin Antibody (PB9920) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9920-images_medium_s1793545824420021figf6.pngAnti-Osteocalcin/BGLAP AntibodyIF staining images of (a) BMP-2, (b) COL-1, (c) OCN, and (d) OPN in BMSCs (red: osteogenic proteins and genes; green: cytoskeleton; blue: nucleus). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.worldscientific.com/doi/abs/10.1142/S1793545824420021'>10.1142/S1793545824420021</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9920-advs71685-fig-0006-m.jpgAnti-Osteocalcin/BGLAP AntibodyIn vitro osteoclast inhibition and osteogenic differentiation evaluation of F-14-TNA. A) Osteogenic differentiation of BMSCs treated with various groups by ALP at 7 days and ARS at 21 days. B) Phalloidin-labeled osteoclast cytoskeleton. C–E) IF analysis revealed RUNX2, OCN, and OPN expression in BMSCs cells. F) WB analysis revealed XB130, p-Src, p-PI3K, and p-Akt expression in BMSCs cells. N = 3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202511045'>40952251</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9920-advs71685-fig-0011-m.jpgAnti-Osteocalcin/BGLAP AntibodyHistological and IF analyses. A) TB staining and IHC staining (OCN) of the bone tissue around the implant at postoperative 2 and 4 weeks. B) TRAP staining of the bone tissue around the implant at postoperative 2 and 4 weeks. C) IF staining of CD68 (M1) and CD206 (M2) for macrophage polarization.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://advanced.onlinelibrary.wiley.com/doi/abs/10.1002/advs.202511045'>40952251</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9920-osteocalcin-primary-antibodies-fc-testing-2.jpgAnti-Osteocalcin/BGLAP Antibody Flow Cytometry analysis of L6 cells using anti-Osteocalcin antibody (PB9920). <br>Overlay histogram showing L6 cells stained with PB9920 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Osteocalcin Antibody (PB9920&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gc1q-r-picoband-trade-antibody-pb9921-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9921-c1qbp-primary-antibodies-wb-testing-1.jpgAnti-GC1q R/C1QBP Antibody Picoband&reg; Western blot analysis of C1QBP using anti-C1QBP antibody (PB9921). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human SW620 whole cell lysates, <br> Lane 3: human 293T whole cell lysates, <br> Lane 4: human A431 whole cell lysates, <br> Lane 5: rat brain tissue lysates, <br> Lane 6: rat spleen tissue lysates, <br> Lane 7: mouse brain tissue lysates, <br> Lane 8: mouse spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C1QBP antigen affinity purified polyclonal antibody (Catalog # PB9921) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C1QBP at approximately 35 kDa. The expected band size for C1QBP is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9921-2-IHC-anti-gc1q-r-picoband-antibody.jpgAnti-GC1q R/C1QBP Antibody Picoband&reg; IHC analysis of C1QBP using anti-C1QBP antibody (PB9921). <br> C1QBP was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C1QBP Antibody (PB9921) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9921-3-IHC-anti-gc1q-r-picoband-antibody.jpgAnti-GC1q R/C1QBP Antibody Picoband&reg; IHC analysis of C1QBP using anti-C1QBP antibody (PB9921). <br> C1QBP was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C1QBP Antibody (PB9921) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9921-4-IHC-anti-gc1q-r-picoband-antibody.jpgAnti-GC1q R/C1QBP Antibody Picoband&reg; IHC analysis of C1QBP using anti-C1QBP antibody (PB9921). <br> C1QBP was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-C1QBP Antibody (PB9921) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9921-5.jpgAnti-GC1q R/C1QBP Antibody Picoband&reg; IF analysis of C1QBP using anti-C1QBP antibody (PB9921).<br> C1QBP was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-C1QBP Antibody (PB9921) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9921-6.pngAnti-GC1q R/C1QBP Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-GC1q R antibody (PB9921). <br> Overlay histogram showing U20S cells stained with PB9921 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GC1q R Antibody (PB9921&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cap1-picoband-trade-antibody-pb9923-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9923-cap1-primary-antibodies-wb-testing-1.jpgAnti-CAP1 Antibody Picoband&reg;Western blot analysis of CAP1 using anti-CAP1 antibody (PB9923). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: rat thymus tissue lysates,<br> Lane 3: rat spleen tissue lysates,<br> Lane 4: mouse thymus tissue lysates,<br> Lane 5: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAP1 antigen affinity purified polyclonal antibody (PB9923) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CAP1 at approximately 55-60 kDa. The expected band size for CAP1 is at 52 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-casr-picoband-trade-antibody-pb9924-boster.html2026-04-03T05:00:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9924-1-WB-anti-casr-calcium-sensing-receptor-picoband-antibody.jpgAnti-Calcium Sensing Receptor/CASR Antibody Picoband&reg; Western blot analysis of CASR using anti-CASR antibody (PB9924). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates,<br> Lane 2: 22RV1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASR antigen affinity purified polyclonal antibody (Catalog # PB9924) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASR at approximately 150 kDa. The expected band size for CASR is at 121 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9924-2-IHC-anti-casr-calcium-sensing-receptor-picoband-antibody.jpgAnti-Calcium Sensing Receptor/CASR Antibody Picoband&reg; IHC analysis of CASR using anti-CASR antibody (PB9924). <br> CASR was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CASR Antibody (PB9924) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9924-3-IHC-anti-casr-calcium-sensing-receptor-picoband-antibody.jpgAnti-Calcium Sensing Receptor/CASR Antibody Picoband&reg; IHC analysis of CASR using anti-CASR antibody (PB9924). <br> CASR was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CASR Antibody (PB9924) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-catalase-picoband-trade-antibody-pb9925-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9925-catalase-primary-antibodies-wb-testing-1_1.jpgAnti-Catalase Antibody Picoband&reg;Western blot analysis of Catalase using anti-Catalase antibody (PB9925). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Jurkat whole cell lysates,<br> Lane 3: human Raji whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 6: mouse 3T3-L1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Catalase antigen affinity purified polyclonal antibody (PB9925) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Catalase at approximately 60 kDa. The expected band size for Catalase is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9925-catalase-primary-antibodies-wb-testing-2.pngAnti-Catalase Antibody Picoband&reg;Western blot analysis of Catalase using anti-Catalase antibody (PB9925). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: Mock group-EV-Monkey MARC-145 whole cell lysates,<br> Lane 2: Mock group-OE-Monkey MARC-145 whole cell lysates,<br> Lane 3: Virus group-EV-Monkey MARC-145 whole cell lysates,<br> Lane 4: Virus group-OE-Monkey MARC-145 whole cell lysates,.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Catalase antigen affinity purified polyclonal antibody (PB9925) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Unitec Cambridge system. A specific band was detected for Catalase at approximately 63 kDa. The expected band size for Catalase is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9925-dmso_a_12471889_f0008_c.jpgAnti-Catalase Antibody Picoband&reg;DGLHT’s effect on liver protein expression Level in T2DM Mice.Three samples were analyzed for each group. The data were analyzed using an unpaired t-test. #P<0.05, ##P<0.01, ###P<0.001 vs Control group, *P<0.05, **P<0.01 vs Model group.Bar graphs show mean ± SD.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.tandfonline.com/doi/abs/10.2147/DMSO.S525913'>41146733</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9925-catalase-primary-antibodies-ihc-testing-1.jpgAnti-Catalase Antibody Picoband&reg;IHC analysis of Catalase using anti-Catalase antibody (PB9925). <br>Catalase was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Catalase Antibody (PB9925) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9925-catalase-primary-antibodies-if-testing-1.jpgAnti-Catalase Antibody Picoband&reg;IF analysis of Catalase using anti-Catalase antibody (PB9925). <br> Catalase was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Catalase Antibody (PB9925) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9925-catalase-primary-antibodies-fcm-testing-1.jpgAnti-Catalase Antibody Picoband&reg;Flow Cytometry analysis of Jurkat cells using anti-Catalase antibody (PB9925). <br> Overlay histogram showing Jurkat cells stained with PB9925 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Catalase Antibody (PB9925, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cct3-picoband-trade-antibody-pb9926-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-wb-testing-1.jpgAnti-CCT3 Antibody Picoband&reg; Western blot analysis of CCT3 using anti-CCT3 antibody (PB9926). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat lung tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT3 antigen affinity purified polyclonal antibody (Catalog # PB9926) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT3 at approximately 61 kDa. The expected band size for CCT3 is at 61 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-fc-testing-2.jpgAnti-CCT3 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-CCT3 antibody (PB9926). <br>Overlay histogram showing K562 cells stained with PB9926 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT3 Antibody (PB9926&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-fc-testing-3.jpgAnti-CCT3 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-CCT3 antibody (PB9926). <br>Overlay histogram showing PC-3 cells stained with PB9926 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT3 Antibody (PB9926&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-fc-testing-4.jpgAnti-CCT3 Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-CCT3 antibody (PB9926). <br>Overlay histogram showing U251 cells stained with PB9926 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT3 Antibody (PB9926&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-ihc-testing-5.jpgAnti-CCT3 Antibody Picoband&reg; IHC analysis of CCT3 using anti-CCT3 antibody (PB9926).<br> CCT3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT3 Antibody (PB9926) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-ihc-testing-6.jpgAnti-CCT3 Antibody Picoband&reg; IHC analysis of CCT3 using anti-CCT3 antibody (PB9926).<br> CCT3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT3 Antibody (PB9926) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-ihc-testing-7.jpgAnti-CCT3 Antibody Picoband&reg; IHC analysis of CCT3 using anti-CCT3 antibody (PB9926).<br> CCT3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT3 Antibody (PB9926) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-ihc-testing-8.jpgAnti-CCT3 Antibody Picoband&reg; IHC analysis of CCT3 using anti-CCT3 antibody (PB9926).<br> CCT3 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT3 Antibody (PB9926) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-9.jpgAnti-CCT3 Antibody Picoband&reg; IF analysis of CCT3 using anti-CCT3 antibody (PB9926).<br> CCT3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT3 Antibody (PB9926) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-10.jpgAnti-CCT3 Antibody Picoband&reg; IF analysis of CCT3 using anti-CCT3 antibody (PB9926).<br> CCT3 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT3 Antibody (PB9926) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9926-cct3-primary-antibodies-wb-testing-2_1.jpgAnti-CCT3 Antibody Picoband&reg;Western blot analysis of CCT3 using anti-CCT3 antibody (PB9926). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1-4: human U2OS cell whole cell lysates, <br> Lane 5-8: human U2OS cell whole cell lysates, <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT3 antigen affinity purified polyclonal antibody (PB9926) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for CCT3 at approximately 61 kDa. The expected band size for CCT3 is at 61 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ada-picoband-trade-antibody-pb9914-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9914-ada-primary-antibodies-wb-testing-1.jpgAnti-ADA Antibody Picoband&reg; Western blot analysis of ADA using anti-ADA antibody (PB9914). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: rat thymus tissue lysates,<br> Lane 3: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody (Catalog # PB9914) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for ADA at approximately 41 kDa. The expected band size for ADA is at 41 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9914-ada-primary-antibodies-ihc-testing-2.jpgAnti-ADA Antibody Picoband&reg; IHC analysis of ADA using anti-ADA antibody (PB9914). <br> ADA was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADA Antibody (PB9914) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9914-ada-primary-antibodies-fcm-testing-3.jpgAnti-ADA Antibody Picoband&reg; Flow Cytometry analysis of Jurkat cells using anti-ADA antibody (PB9914). <br> Overlay histogram showing Jurkat cells stained with PB9914 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADA Antibody (PB9914, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cct4-picoband-trade-antibody-pb9927-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-cct4-primary-antibodies-wb-testing-1.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; Western blot analysis of CCT4 using anti-CCT4 antibody (PB9927). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human U20S whole cell lysates,<br> Lane 3: human SW620 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT4 antigen affinity purified polyclonal antibody (Catalog # PB9927) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT4 at approximately 58 kDa. The expected band size for CCT4 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9927-2-IHC-anti-tcp1-delta-picoband-antibody.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of CCT4 using anti-CCT4 antibody (PB9927).<br> CCT4 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT4 Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9927-3-IHC-anti-tcp1-delta-picoband-antibody.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of CCT4 using anti-CCT4 antibody (PB9927).<br> CCT4 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT4 Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9927-4-IHC-anti-tcp1-delta-picoband-antibody.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of CCT4 using anti-CCT4 antibody (PB9927).<br> CCT4 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT4 Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-ihc-testing-5.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of TCP1 delta using anti-TCP1 delta antibody (PB9927).<br> TCP1 delta was detected in immunocytochemical section of LOVO cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-TCP1 delta Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-ihc-testing-6.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of TCP1 delta using anti-TCP1 delta antibody (PB9927).<br> TCP1 delta was detected in immunocytochemical section of Neuro-2a cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-TCP1 delta Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-ihc-testing-7.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of TCP1 delta using anti-TCP1 delta antibody (PB9927).<br> TCP1 delta was detected in immunocytochemical section of NRK cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-TCP1 delta Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-ihc-testing-8.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of TCP1 delta using anti-TCP1 delta antibody (PB9927).<br> TCP1 delta was detected in immunocytochemical section of SHG-44 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-TCP1 delta Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-ihc-testing-9.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of TCP1 delta using anti-TCP1 delta antibody (PB9927).<br> TCP1 delta was detected in frozen section of mouse brain tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TCP1 delta Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-ihc-testing-10.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IHC analysis of TCP1 delta using anti-TCP1 delta antibody (PB9927).<br> TCP1 delta was detected in frozen section of rat brain tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-TCP1 delta Antibody (PB9927) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-cct4-primary-antibodies-ip-testing-1.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg;Immunoprecipitating (IP) CCT4 in MCF-7 whole cell lysate.<br> Western blot analysis of CCT4 using anti-CCT4 antibody (PB9927); <br> Lane 1: MCF-7 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-CCT4 antibody in MCF-7 whole cell lysate;<br> Lane 3: anti-CCT4 antibody (2μg) + MCF-7 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCT4 antigen affinity purified polyclonal antibody (PB9927) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CCT4 at approximately 58 kDa. The expected band size for CCT4 is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-fc-testing-11.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-CCT4 antibody (PB9927). <br>Overlay histogram showing PC-3 cells stained with PB9927 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT4 Antibody (PB9927&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-fc-testing-12.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-CCT4 antibody (PB9927). <br>Overlay histogram showing U251 cells stained with PB9927 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT4 Antibody (PB9927&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-tcp1_delta-primary-antibodies-fc-testing-13.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-CCT4 antibody (PB9927). <br>Overlay histogram showing K562 cells stained with PB9927 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT4 Antibody (PB9927&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-14.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg; IF analysis of CCT4 using anti-CCT4 antibody (PB9927).<br> CCT4 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT4 Antibody (PB9927) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9927-cct4-primary-antibodies-if-testing-2.jpgAnti-TCP1 delta/CCT4 Antibody Picoband&reg;IF analysis of CCT4 using anti-CCT4 antibody (PB9927). <br> CCT4 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-CCT4 Antibody (PB9927) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cct5-picoband-trade-antibody-pb9928-boster.html2026-04-01T05:01:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9928-cct5-primary-antibodies-wb-testing-1.jpgAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg; Western blot analysis of CCT5 using anti-CCT5 antibody (PB9928). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates,<br> Lane 4: human A549 whole cell lysates,<br> Lane 5: human 293T whole cell lysates,<br> Lane 6: human CACO-2 whole cell lysates,<br> Lane 7: human PC-3 whole cell lysates,<br> Lane 8: human U20S whole cell lysates,<br> Lane 9: rat testis tissue lysates,<br> Lane 10: mouse testis tissue lysates,<br> Lane 11: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody (Catalog # PB9928) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT5 at approximately 60 kDa. The expected band size for CCT5 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9928-2_1-IHC-anti-tcp1-epsilon-picoband-antibody.jpgAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg; IHC analysis of CCT5 using anti-CCT5 antibody (PB9928). <br> CCT5 was detected in paraffin-embedded section of mouse ovary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT5 Antibody (PB9928) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9928-3-IHC-anti-tcp1-epsilon-picoband-antibody.jpgAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg; IHC analysis of CCT5 using anti-CCT5 antibody (PB9928). <br> CCT5 was detected in paraffin-embedded section of rat ovary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT5 Antibody (PB9928) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9928-4-IHC-anti-tcp1-epsilon-picoband-antibody.jpgAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg; IHC analysis of CCT5 using anti-CCT5 antibody (PB9928). <br> CCT5 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT5 Antibody (PB9928) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9928-tcp1_epsilon-primary-antibodies-ihc-testing-5.jpgAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg; IHC analysis of TCP1 epsilon using anti-TCP1 epsilon antibody (PB9928).<br> TCP1 epsilon was detected in immunocytochemical section of LOVO cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-TCP1 epsilon Antibody (PB9928) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9928-tcp1_epsilon-primary-antibodies-if-testing-6.jpgAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg; IF analysis of CCT5 using anti-CCT5 antibody (PB9928).<br> CCT5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT5 Antibody (PB9928) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9928-cct5-primary-antibodies-ip-testing-1.jpgAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg;Immunoprecipitating (IP) CCT5 in MCF-7 whole cell lysate.<br> Western blot analysis of CCT5 using anti-CCT5 antibody (PB9928); <br> Lane 1: MCF-7 whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-CCT5 antibody in MCF-7 whole cell lysate;<br> Lane 3: anti-CCT5 antibody (2μg) + MCF-7 whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody (PB9928) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CCT5 at approximately 60 kDa. The expected band size for CCT5 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9928-tcp1_epsilon-primary-antibodies-fc-testing-7.pngAnti-TCP1 epsilon/CCT5 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-CCT5 antibody (PB9928). <br> Overlay histogram showing A431 cells stained with PB9928 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT5 Antibody (PB9928&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd2-picoband-trade-antibody-pb9929-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9929-1-WB-anti-cd2-picoband-antibody.jpgAnti-CD2/CD2 Antibody Picoband&reg; Western blot analysis of CD2 using anti-CD2 antibody (PB9929). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse cardiac muscle tissue lysates,<br> Lane 2: HEPA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD2 antigen affinity purified polyclonal antibody (Catalog # PB9929) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD2 at approximately 58 kDa. The expected band size for CD2 is at 38 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9929-2-IHC-anti-cd2-picoband-antibody.jpgAnti-CD2/CD2 Antibody Picoband&reg; IHC analysis of CD2 using anti-CD2 antibody (PB9929). <br> CD2 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD2 Antibody (PB9929) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd9-picoband-trade-antibody-pb9930-boster.html2026-03-28T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9930-1-WB-anti-cd9-picoband-antibody.jpgAnti-CD9 Antibody Picoband&reg; Western blot analysis of CD9 using anti-CD9 antibody (PB9930). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # PB9930) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 25 kDa. The expected band size for CD9 is at 25 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9930-cd9-primary-antibodies-ihc-testing-2.jpgAnti-CD9 Antibody Picoband&reg; IHC analysis of CD9 using anti-CD9 antibody (PB9930). <br> CD9 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD9 Antibody (PB9930) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9930-cd9-primary-antibodies-wb-testing-3.jpgAnti-CD9 Antibody Picoband&reg; Western blot analysis of CD9 using anti-CD9 antibody (PB9930). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates, <br> Lane 2: rat lung tissue lysates, <br> Lane 3: mouse spleen tissue lysates, <br> Lane 4: mouse lung tissue lysates, <br> Lane 5: mouse HEPA1-6 whole cell lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD9 antigen affinity purified polyclonal antibody (Catalog # PB9930) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD9 at approximately 25KD. The expected band size for CD9 is at 25KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9930-cd9-primary-antibodies-if-testing-4.jpgAnti-CD9 Antibody Picoband&reg; IF analysis of CD9 using anti-CD9 antibody (PB9930). <br> CD9 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CD9 Antibody (PB9930) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9930-cd9-primary-antibodies-fcm-testing-5.pngAnti-CD9 Antibody Picoband&reg; Flow Cytometry analysis of RAW264.7 cells using anti-CD9 antibody (PB9930). <br>Overlay histogram showing RAW264.7 cells stained with PB9930 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD9 Antibody (PB9930, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd27-picoband-trade-antibody-pb9931-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9931-1-WB-anti-tnfrsf7-cd27-picoband-antibody.jpgAnti-CD27 Antibody Picoband&reg; Western blot analysis of CD27 using anti-CD27 antibody (PB9931). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD27 antigen affinity purified polyclonal antibody (Catalog # PB9931) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD27 at approximately 51 kDa. The expected band size for CD27 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9931-2-IHC-anti-tnfrsf7-cd27-picoband-antibody.jpgAnti-CD27 Antibody Picoband&reg; IHC analysis of CD27 using anti-CD27 antibody (PB9931). <br> CD27 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD27 Antibody (PB9931) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9931-cd27-primary-antibodies-fc-testing-3.pngAnti-CD27 Antibody Picoband&reg;3. Flow Cytometry analysis of A549 cells using anti-CD27 antibody (PB9931).<br>Overlay histogram showing A549 cells stained with PB9931 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD27 Antibody (PB9931&#44;1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9931-cd27-primary-antibodies-if-testing-4.pngAnti-CD27 Antibody Picoband&reg; IF analysis of CD27 using anti-CD27 antibody (PB9931). <br> CD27 was detected in immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD27 Antibody (PB9931) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd33-picoband-trade-antibody-pb9932-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9932-cd33-primary-antibodies-wb-testing-1.jpgAnti-CD33 Antibody Picoband&reg;Western blot analysis of CD33 using anti-CD33 antibody (PB9932). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (PB9932) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD33 at approximately 60-75 kDa. The expected band size for CD33 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9932-cd33-primary-antibodies-ihc-testing-1.jpgAnti-CD33 Antibody Picoband&reg;IHC analysis of CD33 using anti-CD33 antibody (PB9932). <br>CD33 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD33 Antibody (PB9932) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdcp1-picoband-trade-antibody-pb9933-boster.html2026-03-24T05:05:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9933-1-WB-anti-cdcp1-picoband-antibody.jpgAnti-CDCP1 Antibody Picoband&reg; Western blot analysis of CDCP1 using anti-CDCP1 antibody (PB9933). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCP1 antigen affinity purified polyclonal antibody (Catalog # PB9933) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCP1 at approximately 130 kDa. The expected band size for CDCP1 is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9933-2-IHC-anti-cdcp1-picoband-antibody.jpgAnti-CDCP1 Antibody Picoband&reg; IHC analysis of CDCP1 using anti-CDCP1 antibody (PB9933). <br> CDCP1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CDCP1 Antibody (PB9933) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9933-oncotarget-10-2340-g006.jpgAnti-CDCP1 Antibody Picoband&reg;Recruitment of immune cells and evidence of local spread. Paraffin-embedded sections prepared from the intestinal or colonic tumors isolated from untreated (Un), DSS-treated or AOM+DSS-treated Apc1638N/+ mice were subjected to F4/80 (upper panel), CD3/β-catenin co-localization (middle panel) and CD110 and CDCP1 (lower panel) staining, respectively. DAPI was used as nuclear stain. Scale bars = 150-250μm; n = 3 independent experiments.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6481322/&orig_host=www.ncbi.nlm.nih.gov'>31040926</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9933-3-IHC-anti-cdcp1-picoband-antibody.jpgAnti-CDCP1 Antibody Picoband&reg; IHC analysis of CDCP1 using anti-CDCP1 antibody (PB9933). <br> CDCP1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CDCP1 Antibody (PB9933) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9933-4-IHC-anti-cdcp1-picoband-antibody.jpgAnti-CDCP1 Antibody Picoband&reg; IHC analysis of CDCP1 using anti-CDCP1 antibody (PB9933). <br> CDCP1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CDCP1 Antibody (PB9933) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB9933-5.jpgAnti-CDCP1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-CDCP1 antibody (PB9933). <br>Overlay histogram showing PC-3 cells stained with PB9933 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDCP1 Antibody (PB9933&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-factor-b-picoband-trade-antibody-pb9934-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9934-1-WB-anti-factor-b-picoband-antibody.jpgAnti-Factor B/CFB Antibody Picoband&reg; Western blot analysis of Factor B using anti-Factor B antibody (PB9934). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: A549 whole cell lysates,<br> Lane 2: 293T whole cell lysates,<br> Lane 3: HELA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor B antigen affinity purified polyclonal antibody (Catalog # PB9934) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor B at approximately 86 kDa. The expected band size for Factor B is at 86 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9934-2-IHC-anti-factor-b-picoband-antibody.jpgAnti-Factor B/CFB Antibody Picoband&reg; IHC analysis of Factor B using anti-Factor B antibody (PB9934). <br>Factor B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Factor B Antibody (PB9934) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-factor-i-picoband-trade-antibody-pb9935-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9935-1-WB-anti-factor-i-picoband-antibody.jpgAnti-Factor I/CFI Antibody Picoband&reg; Western blot analysis of Factor I using anti-Factor I antibody (PB9935). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates&#44; <br> Lane 2: HELA whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor I antigen affinity purified polyclonal antibody (Catalog # PB9935) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor I at approximately 75KD; 45KD. The expected band size for Factor I is at 66KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9935-factor_i-primary-antibodies-fc-testing-2.jpgAnti-Factor I/CFI Antibody Picoband&reg; Flow Cytometry analysis of U-87 cells using anti-Factor I antibody (PB9935). <br>Overlay histogram showing U-87 cells stained with PB9935 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Factor I Antibody (PB9935&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9935-factor_i-primary-antibodies-fc-testing-3.jpgAnti-Factor I/CFI Antibody Picoband&reg; Flow Cytometry analysis of HEPG2 cells using anti-Factor I antibody (PB9935). <br>Overlay histogram showing HEPG2 cells stained with PB9935 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Factor I Antibody (PB9935&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calcitonin-picoband-trade-antibody-pb9936-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9936-cgrp-primary-antibodies-ihc-testing-1.jpgAnti-Calcitonin/CALCA AntibodyIHC analysis of CGRP using anti-CGRP antibody (PB9936). <br> CGRP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CGRP Antibody (PB9936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9936-cgrp-primary-antibodies-ihc-testing-2.jpgAnti-Calcitonin/CALCA AntibodyIHC analysis of CGRP using anti-CGRP antibody (PB9936). <br> CGRP was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CGRP Antibody (PB9936) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-calcitonin-picoband-trade-antibody-pb9937-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9937-calca-primary-antibodies-ihc-testing-1.jpgAnti-Calcitonin/CALCA AntibodyIHC analysis of Survivin/Calcitonin/CALCA using anti-Survivin/Calcitonin/CALCA antibody (PB9937). <br> Survivin/Calcitonin/CALCA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/Calcitonin/CALCA Antibody (PB9937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9937-calca-primary-antibodies-ihc-testing-2.jpgAnti-Calcitonin/CALCA AntibodyIHC analysis of Survivin/Calcitonin/CALCA using anti-Survivin/Calcitonin/CALCA antibody (PB9937). <br> Survivin/Calcitonin/CALCA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Survivin/Calcitonin/CALCA Antibody (PB9937) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-i-picoband-trade-antibody-pb9938-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-41598_2024_79663_fig6_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;D + Q decreases the renal senescence and renal fibrosis induced by palbociclib in uIRI murine model. Palbociclib was administered to C57BL/6 mice via gavage from day 1 to day 7, then treated with D + Q weekly for a total of two doses. Mice were sacrificed on day 21 following renal ischemia ( A ). Relative mRNA expression levels of P21 and P53 in renal tissue are shown ( B , C ). Representative and quantitative immunoblot analysis of Collagen I and αSMA in renal tissue is depicted ( D - F ). Representative immunohistochemical staining of Sirius Red, Collagen I, and Fibronectin in renal tissue (scale bare, 50 μm) is depicted ( G ). Semi-quantitative analysis of stained renal tissue sections, indicating the positive areas for Sirius Red ( H ), Collagen I ( I ), and Fibronectin ( J ) is provided. The qPCR and immunoblot results are shown as the fold change compared with the Sham group. Data are expressed as mean ± SD. n = 4 mice. ** p < 0.05, *** p < 0.01, **** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-79663-x'>39548257</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-41598_2024_79663_fig5_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Palbociclib increases the renal fibrosis reduced by D + Q in uIRI murine model. Immunoblot analyses illustrating representative blots and quantitative data for α-SMA and Collagen I in renal tissue are shown ( A - C ). Representative blots are presented ( A ), with quantification of α-SMA and Collagen I level shown in ( B ) and ( C ), respectively. Representative immunohistochemical staining of Sirius Red, α-SMA, Collagen I, and Fibronectin in renal tissue (scale bar, 50 μm) is depicted ( D ). Semi-quantitative analysis of stained renal tissue sections, indicating the positive areas for Sirius Red ( E ), α-SMA ( F ), Collagen I ( G ), and Fibronectin ( H ), is provided. The qPCR and immunoblot results are shown as the fold change compared with the Sham group. Data are expressed as mean ± SD. n = 4 for the Sham group and n = 6 for all other groups. * p < 0.05, ** p < 0.01, *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-79663-x'>39548257</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-41598_2024_79663_fig3_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Palbociclib administration post-acute stage of AKI promotes renal senescence and fibrosis. Palbociclib was administered to C57BL/6 mice via gavage from day 8 to day 14, and the mice were euthanized on day 21 post-renal ischemia ( A ). Relative mRNA expression levels of P16 and P21 are shown ( B , C ). Relative mRNA expression levels of Collagen I and Fibronectin are depicted ( D , E ). Representative immunohistochemical staining of α-SMA, Collagen I, and Fibronectin in renal tissue (scale bar, 50 μm) is presented ( F ), along with semi-quantitative analysis of α-SMA, Collagen I, and Fibronectin positive areas in renal tissue ( H , I , J ). Representative immunohistochemical staining of F4/80 (scale bar, 50 μm) is shown ( G ), and semi-quantitative analysis of macrophage infiltration in renal tissue is provided ( K ). The qPCR results are shown as the fold change compared with the Veh group. Data are expressed as mean ± SD. n = 4 mice. * p < 0.05, ** p < 0.01, *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-79663-x'>39548257</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-wb-testing-1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; Western blot analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: rat skin tissue lysates,<br> Lane 3: rat lung tissue lysates,<br> Lane 4: mouse skin tissue lysates,<br> Lane 5: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen I antigen affinity purified polyclonal antibody (Catalog # PB9938) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen I at approximately 130-180 kDa. The expected band size for Collagen I is at 139 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-41598_2024_79663_fig2_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;Palbociclib administration in the acute stage of AKI induces renal senescence and fibrosis. Palbociclib was administered to C57BL/6 mice via gavage from day 1 to day 7, and the mice were euthanized on day 21 post-renal ischemia ( A ). Relative mRNA expression levels of P16 and P21 are shown ( B , C ). Representative immunostaining of SA-β-Gal activity (scale bar, 100 μm) is presented ( D ). Relative mRNA expression levels of Collagen I and Fibronectin are depicted ( E , F ). Representative immunohistochemical staining of Collagen I and Fibronectin in renal tissue (scale bar, 50 μm) is shown ( G ), along with semi-quantitative analysis ( I , J ). Representative immunohistochemical staining of F4/80 (scale bar, 50 μm) is presented ( H ), and semi-quantitative analysis of macrophage infiltration in renal tissue is provided ( K ). The qPCR results are shown as the fold change compared with the Veh group. Data are expressed as mean ± SD. n = 4 mice. * p < 0.05, ** p < 0.01, *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-024-79663-x'>39548257</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-2.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-40164_2025_616_fig7_html.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Decreases in myofibroblast characteristics of MRC-5 by DKK1. ( A ) Volcano plot for secretory genes using RNA-sequencing data from Fig. A. Red dots represent genes satisfying p-value < 0.05 (left). GO term analysis for secreted protein genes satisfying p-value < 0.05 (right). ( B ) Myofibroblast marker genes in MRC-5 treated with HCC827-DKK1-OE culture supernatants. The data were obtained using RNA-sequencing data from Fig. A. ( C ) Col1a1 , ACTA2 mRNA expression in MRC-5 treated with HCC827-DKK1-OE culture supernatants for 6 h. ( D ) Col1a1 and α-SMA protein expression in MRC-5 treated with HCC827-DKK1-OE culture supernatants for 48 h. ( E ) TGF-β concentration in culture media and supernatant obtained from HCC827-LV con and HCC827-DKK1 OE. ( F ) Masson’s trichrome staining using lung tissue obtained from Fig. F. Representative images (left) and collagen area fraction (%) analyzed by ImageJ software (right). ( G ) Protein levels of Col1a1 and a-SMA in tumor sample obtained from Fig. I. All statistical significance of the differences was determined by unpaired two-tailed Student t-test. ns, non-significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11871833/'>40025612</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-3.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-4.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-5.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-6.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-7.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-8.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IHC analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-if-testing-9.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IF analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-if-testing-10.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IF analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-if-testing-11.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg; IF analysis of Collagen I using anti-Collagen I antibody (PB9938). <br> Collagen I was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Collagen I Antibody (PB9938) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-10.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;IHC analysis of COL1A1 using anti-COL1A1 antibody (PB9938).<br> COL1A1 was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-COL1A1 Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-9.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;IHC analysis of COL1A1 using anti-COL1A1 antibody (PB9938).<br> COL1A1 was detected in a paraffin-embedded section of human tonsillitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-COL1A1 Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9938-col1a1-primary-antibodies-ihc-testing-8_1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;IHC analysis of COL1A1 using anti-COL1A1 antibody (PB9938).<br> COL1A1 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-COL1A1 Antibody (PB9938) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-i-picoband-trade-antibody-pb9939-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-col1a1-primary-antibodies-wb-testing-1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Western blot analysis of COL1A1 using anti-COL1A1 antibody (PB9939). <br> Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: rat skin tissue lysates, <br> Lane 3: mouse lung tissue lysates, <br> Lane 4: mouse skin tissue lysates, <br> Lane 5: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL1A1 antigen affinity purified polyclonal antibody (PB9939) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for COL1A1 at approximately 130,180-200 kDa. The expected band size for COL1A1 is at 138 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-10020_2024_1034_fig2_html.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;OST treatment attenuated TiPs-induced osteogenesis inhibition in vivo. ( A ) Representative Masson’s trichrome staining images of calvarial sections. Scale bar, 200 μm (upper), 50 μm (lower). ( B ) Quantitative analysis of new bone area fraction (%) determined by Masson’s trichrome staining. ( C ) Representative images of calcein (green) and alizarin red (red) double labeling with a 10-day interval. Scale bar, 50 μm (upper), 20 μm (lower). ( D ) Quantitative analysis of average periosteum mineral apposition rates (MAR, µm/d). ( E-F ) Representative images and quantitative analysis of immunohistochemical (IHC) staining for osteocalcin (OCN). Scale bar, 100 μm (upper), 50 μm (lower). ( G-H ) Representative images and quantitative analysis of IHC staining for collagen type I alpha 1 (COL1α1). Scale bar, 100 μm (upper), 50 μm (lower). ( I-J ) Western blot analysis of the expression of osteogenic markers (OCN, COL1α1) in calvarial bone tissue samples from each group. n = 6. The selected images reflect typical examples from each group, closely representing the median degree as per statistical analysis. Data are presented as mean ± SD. One-way ANOVA with Tukey’s post hoc test. The relative COL1α1 protein levels were analyzed using Brown-Forsythe and Welch ANOVA tests followed by Tamhane T2 post hoc tests. ** P < 0.01 versus the Sham group. # P < 0.05 and ## P < 0.01 versus the TiPs group. ns , not statistically significant versus the TiPs group<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11660672/'>39707212</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-fphar-13-830312-g001.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Antifibrotic effect of microcystin (MC) in renal fibrosis mice. Mice were treated with MC-LR (20 μg/kg/day) or MC-RR (20 μg/kg/day) by intragastrical administration for 4 weeks in advance, and then unilateral ureteral ligation was performed to construct a mouse model of obstructive renal fibrosis. The operated mice were administrated with MC-LR or MC-RR for another week, and then were euthanized for further analysis. (A) Schematic diagram of the experimental design. Forty mice were divided into four groups, Sham, unilateral ureteral obstruction (UUO), UUO + MC-LR, and UUO + MC-RR groups, each with 10 mice. No mice died during the experiment. (B,C) Kidney tissue sections were employed for H&E and Masson staining (scale bar: 40 μm). (D) Fibrosis positive area in Masson staining were assessed by pathologist blind to this study ( n = 6). (E,F) The protein level of α -smooth muscle actin (α-SMA), fibronectin and collagen I were measured in the obstructive renal tissues of UUO mice by western blot. * p < 0.05, ** p < 0.01 determined by one-way ANOVA with S–N–K post hoc analysis. (G) Residual contents of MC were detected in the kidney tissues of model mice treatment with MC-LR or MC-RR using the ELISA method. Data were analyzed using independent Student’s t -test, * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9218570/&orig_host=www.ncbi.nlm.nih.gov'>35754468</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-fphar-13-830312-g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Renoprotection effect of microcystin (MC)-RR in different doses on unilateral ureteral obstruction (UUO) mice. The regimen of MC-RR was used in UUO mice. Twenty-five mice were divided into five groups, Sham, UUO, and UUO mice with treatment of MC-RR in three different doses (5, 10, and 20 μg/kg/day), each group with 5 mice. (A,B) Kidney tissue sections were employed for H&E and Masson staining (scale bar: 40 μm). (C) Fibrosis positive area in Masson staining were assessed by pathologist blind to this study ( n = 5). (D,E) Protein level of α -smooth muscle actin (α-SMA), fibronectin, and collagen I were measured in the renal tissues of UUO mice by western blot. (F,G) Protein expression of TGF-β, p-Smad3, and Smad3 in kidney tissue was measured by western blot. * p < 0.05, ** p < 0.01 determined by one-way ANOVA with S–N–K post hoc analysis.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9218570/&orig_host=www.ncbi.nlm.nih.gov'>35754468</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-fvets-09-855405-g0001.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Morphological features and identification of primary cultured tibial osteoblasts of broiler chicks. (A) Morphological image of P0 cells, day of 3 culture; (B) morphological image of P1 cells, day of 2 culture; (C) morphological image of P1 cells, day of 10 culture; (D) primary osteoblasts were positive in ALP straining; (E) immunofluorescence of COL1A1 in the primary tibial osteoblasts; (F) mineralized nodules were positive in ARS staining. ALP, alkaline phosphatase; COL1A1, collagen type I alpha 1; ARS, alizarin res S.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8983115/&orig_host=www.ncbi.nlm.nih.gov'>35392115</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-fphar-13-808576-g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;KD inhibits the expression of the fibrosis-related proteins in the rat model (A) The results of the IHC staining displayed the expression of α -SMA of the liver histology slices. KD significantly reduced the expression of brown stained α -SMA after irradiation (B) The IHC scores for grading the expression of α -SMA (C) Expression and localization of collagen in the liver tissue. The red stain represented collagen, which indicated that collagen expression in the IR + KD group was significantly lower than that in the IR group (D-F) The results of the western blots showed the expression of α -SMA and FN proteins in the liver tissue. The administration of KD after irradiation resulted in decreased expression of a -SMA and FN. For all results in this figure, original magnification, ×100 and ×200. Mean ± SEM. n. s. denotes not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC8814438/&orig_host=www.ncbi.nlm.nih.gov'>35126163</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-fbioe-08-00446-g002.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Fluorescence images of tenocytes after 5 days of culture on the surface of a culture plate (control group) and acellular amniotic membrane (amnion group). Tenocytes presented a clear cytoskeleton, good biocompatibility with acellular amniotic membrane, and even distribution on the surface of the materials, showing better growth activity than the control group (A,B) . Cell viability was measured by CCK-8, and the proliferation curve of tenocytes in the control and amniotic membrane groups was drawn (C) . Western blot assay for collagen I, fibronectin, TGF-β1, and bFGF expression in the tenocytes of the control and amnion groups for 1 week (D,E) . * p < 0.05; ** p < 0.01; and *** p < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00446/full'>32478059</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-fbioe-08-00446-g003.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Representative immunofluorescence images of collagen I. Fluorescent micrographs of tenocytes after 2 and 5 days of culture on the surface of a culture plate and acellular amniotic membrane. Tenocyte nucleus shape observed under a fluorescence microscope (A,D,G,J) . Collagen I presented positive after the fluorescent FITC mark was observed under a fluorescence microscope (B,E,H,K) . Tenocyte nucleus and collagen I merging (C,F,I,L) . The corresponding semi quantitative analysis of collagen fluorescence intensity in panels (M,N) (scale bar = 50 um, n = 5, * P < 0.05 and ** P < 0.01).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/bioengineering-and-biotechnology/articles/10.3389/fbioe.2020.00446/full'>32478059</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-fphar-08-00929-g009.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;Evaluation of the effect of CGA on liver histopathological and immunohistochemistry (IHC) in liver tissue. (A) Histological images of rat livers stained with H&E (original magnification, ×200). (B) The histopathologic detection of collagen in the liver by Masson’s trichrome stain (original magnification, ×100). (C,D) Effects of CGA on α-SMA and collagen I expression were examined with immunohistochemistry in liver tissue (original magnification, ×100).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2017.00929/full'>29311932</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-13020_2025_1133_fig1_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;VDR deficiency exacerbated hepatic fibrosis and steatosis in mice. A mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). B mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 5). C Western blot analysis of VDR and TGFβ/Smad pathway (n = 3), D Serum levels of liver function markers (AST, ALT, ALP, and TBIL) (n = 5). * P < 0.05, ** P < 0.01 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01133-x'>40514735</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-13020_2025_1133_fig2_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;VDR activation attenuated TGF-β1-induced fibrosteatotic changes in HSCs. A Western blot analysis of VDR and α-SMA (n = 3). B mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). C mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 6). * P < 0.05, ** P < 0.01 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01133-x'>40514735</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-13020_2025_1133_fig3_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;SI inhibited TGF-β1-induced activation of HSCs. A Chemical structure of the SI. B Cell viability of HSCs. C mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). D Immunofluorescence analysis of α-SMA. scale bar = 20 μm. (n = 6). E Western blot analysis of α-SMA. F Molecular docking results of VDR and SI. G CETSA analysis. (n = 3) * P < 0.05, ** P < 0.01 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01133-x'>40514735</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-13020_2025_1133_fig4_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;SI ameliorated CCl 4 -induced liver fibrosis in mice. A Serum levels of liver function markers (AST, ALT, ALP, and TBIL). B Liver tissue photograph and histopathological analysis (HE, Sirius Red, Masson), scale bar = 200 μm. C Serum calcium concentrations. D mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). (n = 5) * P < 0.05, ** P < 0.01 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01133-x'>40514735</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-13020_2025_1133_fig5_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;SI regulated VDR and fatty acid metabolism to suppress TGFβ/Smad pathway in CCl 4 -induced mice. A Immunohistochemistry analysis of TGF-β1, p-Smad3, α-SMA, COL1 A1. scale bar = 200 μm. (n = 5). B Western blot analysis of VDR, TGF-β1, and α-SMA. C mRNA levels of Cpt1a . D mRNA levels of lipogenic enzymes ( Fasn , Acc-1 , and Acly ) and fatty acid desaturases ( Fads1/2 and Scd1 ) (n = 6) * P < 0.05, ** P < 0.01 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01133-x'>40514735</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-13020_2025_1133_fig7_html.pngAnti-Collagen I/COL1A1 Antibody Picoband&reg;VDR knockout abolished the SI-induced inhibition of HSCs activation. A Western blot analysis (n = 3). B Immunofluorescence analysis of α-SMA. scale bar = 20 μm. (n = 6). C mRNA levels of fibrogenic markers ( α-Sma , Col1a1 , and Timp1 ). (n = 6) *P < 0.05, **P < 0.01 compared with si-NC + TGF-β1; $ P < 0.05, $$ P < 0.01 compared with si-NC; # P < 0.05, ## P < 0.01 compared with si-VDR + TGF-β1 Full size image<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-025-01133-x'>40514735</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-col1a1-primary-antibodies-ihc-testing-1.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939). <br> COL1A1 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-col1a1-primary-antibodies-ihc-testing-2.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939). <br> COL1A1 was detected in a paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9939-col1a1-primary-antibodies-ihc-testing-3.jpgAnti-Collagen I/COL1A1 Antibody Picoband&reg;IHC analysis of COL1A1 using anti COL1A1 antibody (PB9939). <br> COL1A1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-COL1A1 Antibody (PB9939) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-a-crystallin-picoband-trade-antibody-pb9940-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9940-1-WB-anti-alpha-a-crystallin-picoband-antibody.jpgAnti-Alpha A Crystallin/CRYAA Antibody Picoband&reg; Western blot analysis of CRYAA using anti-CRYAA antibody (PB9940). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates&#44; <br> Lane 2: mouse eye ball tissue lysates&#44; <br> Lane 3: SMMC-7721 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRYAA antigen affinity purified polyclonal antibody (Catalog # PB9940) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRYAA at approximately 20KD. The expected band size for CRYAA is at 20KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9940-2-IHC-anti-alpha-a-crystallin-picoband-antibody.jpgAnti-Alpha A Crystallin/CRYAA Antibody Picoband&reg; IHC analysis of CRYAA using anti-CRYAA antibody (PB9940). <br> CRYAA was detected in paraffin-embedded section of mouse spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRYAA Antibody (PB9940) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9940-3-IHC-anti-alpha-a-crystallin-picoband-antibody.jpgAnti-Alpha A Crystallin/CRYAA Antibody Picoband&reg; IHC analysis of CRYAA using anti-CRYAA antibody (PB9940). <br> CRYAA was detected in paraffin-embedded section of rat spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRYAA Antibody (PB9940) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9940-4.pngAnti-Alpha A Crystallin/CRYAA Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-CRYAA antibody (PB9940). <br> Overlay histogram showing HepG2 cells stained with PB9940 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRYAA Antibody (PB9940&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gro-gamma-picoband-trade-antibody-pb9941-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9941-1-WB-anti-cxcl3-picoband-antibody.jpgAnti-GRO gamma/CXCL3 Antibody Picoband&reg; Western blot analysis of GRO gamma using anti-GRO gamma antibody (PB9941). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse lung tissue lysates,<br> Lane 2: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRO gamma antigen affinity purified polyclonal antibody (Catalog # PB9941) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRO gamma at approximately 25 kDa. The expected band size for GRO gamma is at 11 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fabp2-i-fabp-picoband-trade-antibody-pb9943-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9943-1_1-WB-anti-fabp2-i-fabp-picoband-antibody.jpgAnti-Intestinal FABP/FABP2 Antibody Picoband&reg; Western blot analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP2/I-FABP antigen affinity purified polyclonal antibody (Catalog # PB9943) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP2/I-FABP at approximately 15 kDa. The expected band size for FABP2/I-FABP is at 15 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9943-2-IHC-anti-fabp2-i-fabp-picoband-antibody.jpgAnti-Intestinal FABP/FABP2 Antibody Picoband&reg; IHC analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943). <br>FABP2/I-FABP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP2/I-FABP Antibody (PB9943) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9943-41467_2021_27421_fig2_html.pngAnti-Intestinal FABP/FABP2 Antibody Picoband&reg;High fat diet mice physiologic parameters and enrichment of m 6 Am-modified genes in GO terms associated with metabolic processes. a Gain in weight of mice fed a high fat diet (HFD) or regular chow diet across weeks. N = 10 (5 HDF mice, and 5 Chow lean control mice), error bars denote SEM. b NMR measurement of lean and fat mass body composition, indicating that the gain in weight was mainly in fat mass. N = 10 (5 HDF mice, and 5 Chow lean control mice). c Fold change of read end counts (IP/Input) within a sliding window of 5 base-pairs around a reported adenosine TSS in the Fabp2 gene in lean (chow) and HFD mice. All adenosines in the sequence are indicated. d Sequence logo of the m 6 Am peaks around and upstream the annotated TSS within the DNA, portraying the canonical m 6 Am genomic consensus. e . Gene ontology analysis of m 6 Am and CDS m 6 A (>50 nt of TSS) showing a clear enrichment of m 6 Am- but not in m 6 A-modified genes, in GO terms associated with metabolic processes. Source data are provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-021-27421-2'>34893620</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9943-41467_2021_27421_fig3_html.pngAnti-Intestinal FABP/FABP2 Antibody Picoband&reg;m 6 Am significantly regulates mRNA and protein levels upon high fat diet. a mRNA expression heatmap of >1.5 fold differentially expressed genes with differential m 6 Am methylation in HFD. Heatmap colors represent differential mRNA expression fold-change levels in the respective gene between mice samples (green - lower expression; red - higher expression). Right panel indicates if m 6 Am was gained (light red - HFD specific) or lost (light green - chow specific) in HFD. b Heatmap quantification of the mRNA differentially expressed genes that gained or lost m 6 Am upon HFD. Two-tailed p -value of the Chi-square test is reported. c Log protein expression levels of m 6 Am or m 6 A methylated genes versus non-methylated genes in lean control (chow) mice. Two-tailed p -values of the Mann Whitney U test are reported, covering 2246, 1998, and 5737 genes with the respective locations. Box plots surround the 1–3 quartiles, whiskers denote 1.5 interquartile range. d Full proteomic profiling proportions of genes with protein differential expression >1.5 fold, which gained or lost m 6 Am upon HFD. Two-tailed p -value of the Chi-square test is reported. Full proteomic abundance profiling was conducted on two HFD mice samples and two lean chow control mice samples covering a total of 5914 proteins detected. e Western blot of Fabp2 and Fabp5 genes, which were found decorated with m 6 Am only in lean mice. The results show a clear repeating pattern of overexpression across all lean biological replicates ( N = 5) versus all fat biological replicates ( N = 4). See Supplementary Fig. for the quantification of these signals. The available molecular weight markers can be seen within the full scan blots which are available as Source data accompanying this figure, provided as a Source Data file. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41467-021-27421-2'>34893620</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9943-3-IHC-anti-fabp2-i-fabp-picoband-antibody.jpgAnti-Intestinal FABP/FABP2 Antibody Picoband&reg; IHC analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943). <br>FABP2/I-FABP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP2/I-FABP Antibody (PB9943) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9943-4-IHC-anti-fabp2-i-fabp-picoband-antibody.jpgAnti-Intestinal FABP/FABP2 Antibody Picoband&reg; IHC analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943). <br>FABP2/I-FABP was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP2/I-FABP Antibody (PB9943) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9943-5.jpgAnti-Intestinal FABP/FABP2 Antibody Picoband&reg; IF analysis of Intestinal FABP using anti-Intestinal FABP antibody (PB9943).<br> Intestinal FABP was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Intestinal FABP Antibody (PB9943) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf1-picoband-trade-antibody-pb9944-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9944-1_1-WB-anti-fgf1-fgf-acidic-picoband-antibody.jpgAnti-FGF1 Antibody Picoband&reg; Western blot analysis of GADD45A using anti-GADD45A antibody (PB9944). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat cardiac muscle tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45A antigen affinity purified polyclonal antibody (Catalog # PB9944) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45A at approximately 24 kDa. The expected band size for GADD45A is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9944-2_1-IHC-anti-fgf1-fgf-acidic-picoband-antibody.jpgAnti-FGF1 Antibody Picoband&reg; IHC analysis of GADD45A using anti-GADD45A antibody (PB9944). <br>GADD45A was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GADD45A Antibody (PB9944) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9944-3_1-IHC-anti-fgf1-fgf-acidic-picoband-antibody.jpgAnti-FGF1 Antibody Picoband&reg; IHC analysis of GADD45A using anti-GADD45A antibody (PB9944). <br>GADD45A was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GADD45A Antibody (PB9944) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9944-4_1-IHC-anti-fgf1-fgf-acidic-picoband-antibody.jpgAnti-FGF1 Antibody Picoband&reg; IHC analysis of GADD45A using anti-GADD45A antibody (PB9944). <br>GADD45A was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-GADD45A Antibody (PB9944) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gadd45a-picoband-trade-antibody-pb9945-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9945-1-WB-anti-gadd45a-gadd-45alpha-picoband-antibody.jpgAnti-GADD45A Antibody Picoband&reg; Western blot analysis of GADD45A using anti-GADD45A antibody (PB9945). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45A antigen affinity purified polyclonal antibody (Catalog # PB9945) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45A at approximately 19 kDa. The expected band size for GADD45A is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9945-12951_2020_656_fig4_html.pngAnti-GADD45A Antibody Picoband&reg;The mechanism behind the LDEVs effect on gastric cancer cells. a KEGG pathway analysis of SGC-7901 cells after LDEVs treatment for 6 h and b 12 h. The highest KEGG pathways were labeled by red line respectively. c RT-PCR assay the relative expression of GADD45A in AGS, BGC-823, and SGC-7901 cells treated with LDEVs; d Western blot analysis of GADD45α protein expression in three gastric cancer cells; e Flow cytometry assay the intracellular ROS levels using DCFH-DA; f Cell viability of control group, LDEVs group, NAC + LDEVs group, and NAC groups respectively (* p < 0.05; ** p < 0.01, *** p < 0.001, **** p < 0.0001) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12951-020-00656-9'>32690102</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gip-picoband-trade-antibody-pb9946-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9946-gip-primary-antibodies-wb-testing-1.jpgAnti-GIP Antibody Picoband&reg; Western blot analysis of GIP using anti-GIP antibody (PB9946). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIP antigen affinity purified polyclonal antibody (Catalog # PB9946) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIP at approximately 17 kDa. The expected band size for GIP is at 17 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9946-gip-primary-antibodies-ihc-testing-2.jpgAnti-GIP Antibody Picoband&reg; IHC analysis of GIP using anti-GIP antibody (PB9946). <br> GIP was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIP Antibody (PB9946) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9946-gip-primary-antibodies-ihc-testing-3.jpgAnti-GIP Antibody Picoband&reg; IHC analysis of GIP using anti-GIP antibody (PB9946). <br> GIP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIP Antibody (PB9946) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9946-41598_2016_article_bfsrep34284_fig7_html.jpgAnti-GIP Antibody Picoband&reg;Alterations of the protein levels of the components of the GSK-3β—β-catenin—TCF7L2—GLP-1 axis under varying glucose conditions. ( A ) Molecular structure of Emodin. ( B ) Relative protein levels of p-GSK-3β, GSK-3β, β-catenin and TCF7L2 in Control and Emodin groups under different glucose conditions, detected by the western blot analysis. ( C ) Relative mRNA expression of β-catenin and TCF7L2 in Control and Emodin groups under different glucose conditions, detected by real-time PCR. ( D ) GLP-1 and GIP concentration measured by ELISA in the cell supernatant in Control and Emodin groups under different glucose conditions. *p < 0.05, **p < 0.01 vs control under no glucose, # p < 0.05, ## p < 0.01 vs control under low glucose, &p < 0.05, && p < 0.01 vs control under high glucose; mean ± SEM. ( E,F ) Inhibition of TCF7L2 and GSK-3β abolished DMC’s effects. ( E ) Effects of emodin (the main component of DMC) and SB216763 (a specific GSK-3β inhibitor) on the protein levels of β-catenin and TCF7L2 under HG (high glucose) conditions. ( F ) Relative mRNA expression of β-catenin and TCF7L2 in the three groups. ( G ) Relative protein expression of TCF7L2 in HG, HG + emodin and HG + emodin + TCF7L2 SiRNA groups. ( H ) GLP-1 and GIP concentration measured by ELISA in the cell supernatant in the three groups. *p < 0.05, **p < 0.01 vs HG, #p < 0.05, ##p < 0.01 vs HG + Emodin; mean ± SEM. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep34284'>27721485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9946-41598_2016_article_bfsrep34284_fig4_html.jpgAnti-GIP Antibody Picoband&reg;GLP-1 and GIP concentrations in the serum and mRNA levels of GCG and GIP. ( A ) GLP-1 concentrations in the serum in the in the Control, DM and DMC groups. ( B ) GCG (the gene encoding GLP-1) mRNA levels in ileum. ( C ) GIP concentrations in the serum in the five groups. ( D ) GIP mRNA levels in ileum. ( E ) Insulin levels in the serum in the five groups. *p < 0.05, **p < 0.01 vs Control, # p < 0.05, ## p < 0.01 vs DM; mean ± SEM. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep34284'>27721485</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9946-41598_2016_article_bfsrep34284_fig3_html.jpgAnti-GIP Antibody Picoband&reg;Expression of GLP-1 and GIP in ileum. ( A ) Images (×400) of immunofluorescence staining of GLP-1 (glucagon-like peptide-1) and GIP (gastric inhibitory polypeptide) expression in ileum in the Control, DM and DMC groups. ( B ) Immunofluorescence results (×400) indicating GIP expression. (scale bar: 20μm). ( C ) Quantification of the GLP-1 fluorescence intensity (integrated density per stained area). ( D ) Quantification of the GIP fluorescence intensity (integrated density per stained area). *p < 0.05 vs Control, # p < 0.05 vs DM; mean ± SEM. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fsrep34284'>27721485</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gip-picoband-trade-antibody-pb9947-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9947-gip-primary-antibodies-wb-testing-1.jpgAnti-GIP Antibody Picoband&reg; Western blot analysis of GIP using anti-GIP antibody (PB9947). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse stomach tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIP antigen affinity purified polyclonal antibody (Catalog # PB9947) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIP at approximately 18 kDa. The expected band size for GIP is at 16 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9947-gip-primary-antibodies-ihc-testing-2.jpgAnti-GIP Antibody Picoband&reg; IHC analysis of GIP using anti-GIP antibody (PB9947). <br> GIP was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIP Antibody (PB9947) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9947-gip-primary-antibodies-ihc-testing-3.jpgAnti-GIP Antibody Picoband&reg; IHC analysis of GIP using anti-GIP antibody (PB9947). <br> GIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GIP Antibody (PB9947) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-il7r-alpha-picoband-trade-antibody-pb9948-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9948-1-WB-anti-il7r-cd127-picoband-antibody.jpgAnti-IL7R alpha Antibody Picoband&reg; Western blot analysis of IL7R alpha using anti-IL7R alpha antibody (PB9948). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: 22RV1 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL7R alpha antigen affinity purified polyclonal antibody (Catalog # PB9948) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL7R alpha at approximately 70KD. The expected band size for IL7R alpha is at 70KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9948-2.pngAnti-IL7R alpha Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-IL7R alpha antibody (PB9948). <br> Overlay histogram showing U20S cells stained with PB9948 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL7R alpha Antibody (PB9948&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9948-3.pngAnti-IL7R alpha Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-IL7R alpha antibody (PB9948). <br> Overlay histogram showing U87 cells stained with PB9948 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL7R alpha Antibody (PB9948&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-integrin-linked-ilk-picoband-trade-antibody-pb9949-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9949-1-WB-anti-integrin-linked-ilk-picoband-antibody.jpgAnti-Integrin linked ILK Antibody Picoband&reg; Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (PB9949). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates,<br> Lane 2: mouse liver tissue lysates,<br> Lane 3: HELA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin linked ILK antigen affinity purified polyclonal antibody (Catalog # PB9949) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 55 kDa. The expected band size for Integrin linked ILK is at 51 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-isg15-picoband-trade-antibody-pb9950-boster.html2026-03-24T05:05:39+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9950-1-WB-anti-isg15-ucrp-picoband-antibody.jpgAnti-ISG15 Antibody Picoband&reg; Western blot analysis of ISG15 using anti-ISG15 antibody (PB9950). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: 22RV1 whole cell lysates,<br> Lane 2: HELA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISG15 antigen affinity purified polyclonal antibody (Catalog # PB9950) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ISG15 at approximately 15 kDa. The expected band size for ISG15 is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9950-2-IHC-anti-isg15-ucrp-picoband-antibody.jpgAnti-ISG15 Antibody Picoband&reg; IHC analysis of ISG15 using anti-ISG15 antibody (PB9950). <br>ISG15 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ISG15 Antibody (PB9950) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-isg15-picoband-trade-antibody-pb9951-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-isg15-primary-antibodies-wb-testing-1.jpgAnti-ISG15 Antibody Picoband&reg; Western blot analysis of ISG15/Ucrp using anti-ISG15/Ucrp antibody (PB9951). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ISG15/Ucrp antigen affinity purified polyclonal antibody (Catalog # PB9951) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ISG15/Ucrp at approximately 17 kDa. The expected band size for ISG15/Ucrp is at 18 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9951-2-IHC-anti-isg15-ucrp-picoband-antibody.jpgAnti-ISG15 Antibody Picoband&reg; IHC analysis of ISG15/Ucrp using anti-ISG15/Ucrp antibody (PB9951).<br> ISG15/Ucrp was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ISG15/Ucrp Antibody (PB9951) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g006.jpgAnti-ISG15 Antibody Picoband&reg;ISG15 contributed to TECs injury in an ISGylation‐dependent manner. (A) Western blot analysis and densitometric quantification of cGAS, STING, TBK1, p‐TBK1, p65, p‐p65 expression in TECs ( n = 3). (B) Western blot analysis and densitometric quantification of NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N expression in TECs ( n = 3). (C) Flow cytometry analysis and quantitative data depicting the Annexin V/PI double‐positive cells rate ( n = 3). (D) CCK‐8 activity assay quantified cell viability ( n = 3). (E–G) Levels of ROS (E), LDH (F), IL‐18 (G) in TECs ( n = 3). (H) Western blot analysis and densitometric quantification of KIM1, α‐SMA and Vimentin expression in TECs ( n = 3). (I) Relative mRNA level of pro‐inflammatory factors ( Il6 , Tnfa , Mcp1 and Il18 ) in TECs ( n = 3). TECs were transfected with empty vector, ISG15AA or ISG15 (4 µg). Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g009.jpgAnti-ISG15 Antibody Picoband&reg;ISG15 promoted STING signalling via cytosolic mtDNA and established a positive feedback loop. (A) Western blot analysis and densitometric quantification of cGAS, STING, TBK1, p‐TBK1, p65, p‐p65 expression in WT and KO mice treated with vehicle or STZ ( n = 6). (B and C) Western blot analysis and densitometric quantification of cGAS, STING, TBK1, p‐TBK1, p65, p‐p65 expression in TECs ( n = 3). TECs were transfected with mtDNA (4 µg) or si‐ Isg15 (50 nM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g004.jpgAnti-ISG15 Antibody Picoband&reg;ISG15–STING loop‐maintained HG‐induced injury in TECs. (A) Western blot analysis and densitometric quantification of NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD and GSDMD‐N expression in TECs ( n = 3). (B) Flow cytometry analysis and quantitative data depicting the Annexin V/PI double‐positive cells rate ( n = 3). (C–E) Levels of ROS (C), IL‐18 (D), LDH (E) in TECs ( n = 3). TECs were transfected with oe‐STING (4 µg) or si‐ Isg15 (50 nM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g011.jpgAnti-ISG15 Antibody Picoband&reg;ISG15 was involved in HG‐induced mitochondrial impairment and mtDNA release. (A) Representative TEM images of kidney tissues from WT and KO mice treated with STZ ( n = 6). (B) Western blot analysis ISG15/ISGylation expression in TECs treated with vehicle or HG ( n = 3). (C–E) Flow cytometry analysis and quantitative data depicting the mitochondrial membrane potential (C), mitochondrial mass (D) and mtROS (E) ( n = 3). (F and G) qPCR analysis the mtDNA ( Loop1‐3 and mt‐Nd4 ) copy number in the cytosolic compartments (F) and mitochondria (G) ( n = 3). TECs were treated with vehicle or C‐176 (10 µM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001; ns, not significant.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g010.jpgAnti-ISG15 Antibody Picoband&reg;The cGAS–STING pathway was activated in the DKD mice. (A) Western blot analysis and densitometric quantification of cGAS, STING, TBK1, p‐TBK1, p65, p‐p65 expression in DKD mice ( n = 6). (B) Western blot analysis and densitometric quantification of NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N expression in TECs ( n = 3). (C) Western blot analysis ISG15/ISGylation expression in TECs ( n = 3). (D) Flow cytometry analysis and quantitative data depicting the Annexin V/PI double‐positive cells rate ( n = 3). (E–G) Levels of LDH (E), IL‐18 (F), ROS (G) in TECs ( n = 3). (H) Relative mRNA level of pro‐inflammatory factors ( Il6 , Tnfa , Mcp1 , Il18 ) in TECs ( n = 3). (I) Western blot analysis and densitometric quantification of KIM1, α‐SMA and Vimentin expression in TECs ( n = 3). TECs were transfected with vehicle or C‐176 (10 µM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g007.jpgAnti-ISG15 Antibody Picoband&reg;Inhibition of pyroptosis blocked TECs damage and fibrosis induced by ISG15. (A) Flow cytometry analysis and quantitative data depicting the Annexin V/PI double‐positive cells rate ( n = 3). (B and C) Levels of LDH (B), IL‐18 (C) in TECs ( n = 3). (D) Western blot analysis and densitometric quantification of GSDMD, GSDMD‐N, KIM1, α‐SMA and Vimentin expression in TECs ( n = 3). (E) Relative mRNA level of pro‐inflammatory factors ( Il6 , Tnfa , Mcp1 and Il18 ) in TECs ( n = 3). TECs were transfected with si‐ Gsdmd (50 nM) or oe‐ISG15 (4 µg), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g008.jpgAnti-ISG15 Antibody Picoband&reg;Ablation of ISG15 decreased pyroptosis of TECs under HG stimulation. (A) Western blot analysis and densitometric quantification of pyroptosis‐related proteins (NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N) expression in kidney tissues from WT and KO mice treated with vehicle or STZ ( n = 6). (B) Level of IL‐18 in the serum from WT and KO mice treated with vehicle or STZ ( n = 6). (C) Western blot analysis of pyroptosis‐related proteins (NLRP3, Pro‐CASP1, Cleaved‐CASP1, GSDMD, GSDMD‐N) expression in TECs ( n = 3). (D) Flow cytometry analysis and quantitative data depicting the TECs Annexin V/PI double‐positive cells rate ( n = 3). (E) CCK‐8‐kit activity assay quantified cell viability ( n = 3). (F–I) Level of LDH (F), IL‐18 (G), ROS (H and I) in TECs ( n = 3). TECs were transfected with sinc (50 nM) or si‐ Isg15 (50 nM), and then cultured in HG medium for 48 h. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g001.jpgAnti-ISG15 Antibody Picoband&reg;ISG15 deletion was protective against renal injury. (A) Western blot analysis and densitometric quantification of ISG15 expression in kidney tissues from WT and KO mice ( n = 6). (B–E) BUN (B), UAER (C), FBG (D) and OGTT (E) levels in WT and KO mice treated with vehicle or STZ ( n = 6). (F) Representative images of MASSON and PAS staining of the kidney ( n = 6). (G) Western blot analysis and densitometric quantification of KIM1, α‐SMA and Vimentin expression in kidney tissues from WT and KO mice treated with vehicle or STZ ( n = 6). (H) Relative mRNA level of pro‐inflammatory factors ( Il6 , Tnfa, Mcp1 and Il18 ) in the kidney tissues from WT and KO mice treated with vehicle or STZ ( n = 6). (I) Western blot analysis and densitometric quantification of ISG15/ISGylation, KIM1, α‐SMA and Vimentin expression in TECs ( n = 3). (J) Relative mRNA level of pro‐inflammatory factors ( Il6, Tnfa, Mcp1 and Il18 ) in TECs ( n = 3). TECs were transfected with sinc (50 nM) or si‐ Isg15 (50 nM), and then cultured in HG medium for 48 h. BUN, blood urea nitrogen; UAER, urinary albumin excretion rates; FBG, fasted blood; OGTT, oral glucose tolerance. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9951-ctm2-15-e70337-g003.jpgAnti-ISG15 Antibody Picoband&reg;High ISG15 expression in DKD mice. (A) Relative mRNA level of Isg15 in the kidney cortical tissues from WT mice and STZ/HFD‐induced DKD mice ( n = 6). (B) Western blot analysis and quantification of ISG15/ISGylation and KIM‐1 expression in kidney cortical tissues from WT mice and STZ/HFD‐induced DKD mice ( n = 6). (C) Relative mRNA level of Isg15 in the kidney from db/m, 16 W db/db and 24 W db/db mice ( n = 6). (D) Western blot analysis and quantification of ISG15/ISGylation and KIM‐1 expression in kidney cortical tissues from db/m, 16 W db/db and 24 W db/db mice ( n = 6). (E) Relative mRNA level of Isg15 in the TECs cultured in normal medium, MA or HG for 48 h ( n = 3). (F) Western blot analysis and quantification of ISG15/ISGylation and KIM‐1 expression in the TECs ( n = 3). DKD, diabetic kidney disease; HFD, high‐fat diet; HG, high glucose; STZ, streptozotocin; MA, mannitol. Results are expressed as the mean ± SD. * p < .05; ** p < .01; *** p < .001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC12134392/'>40462493</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gal4-picoband-trade-antibody-pb9952-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9952-1-WB-anti-galectin-4-picoband-antibody.jpgAnti-GAL4/LGALS4 Antibody Picoband&reg; Western blot analysis of GAL4 using anti-GAL4 antibody (PB9952). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates,<br> Lane 2: COLO320 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAL4 antigen affinity purified polyclonal antibody (Catalog # PB9952) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAL4 at approximately 39 kDa. The expected band size for GAL4 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9952-2.jpgAnti-GAL4/LGALS4 Antibody Picoband&reg; IF analysis of GAL4 using anti-GAL4 antibody (PB9952). <br> GAL4 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The celss were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GAL4 Antibody (PB9952) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9952-3.pngAnti-GAL4/LGALS4 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-GAL4 antibody (PB9952). <br> Overlay histogram showing THP-1 cells stained with PB9952 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GAL4 Antibody (PB9952&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mef2a-picoband-trade-antibody-pb9953-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9953-1-WB-anti-mef2a-picoband-antibody.jpgAnti-MEF2A Antibody Picoband&reg; Western blot analysis of MEF2A using anti-MEF2A antibody (PB9953). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: HELA whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEF2A antigen affinity purified polyclonal antibody (Catalog # PB9953) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEF2A at approximately 56 kDa. The expected band size for MEF2A is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9953-2-IHC-anti-mef2a-picoband-antibody.jpgAnti-MEF2A Antibody Picoband&reg; IHC analysis of MEF2A using anti-MEF2A antibody (PB9953). <br>MEF2A was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MEF2A Antibody (PB9953) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9953-3-IHC-anti-mef2a-picoband-antibody.jpgAnti-MEF2A Antibody Picoband&reg; IHC analysis of MEF2A using anti-MEF2A antibody (PB9953). <br>MEF2A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MEF2A Antibody (PB9953) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9953-4-IHC-anti-mef2a-picoband-antibody.jpgAnti-MEF2A Antibody Picoband&reg; IHC analysis of MEF2A using anti-MEF2A antibody (PB9953). <br>MEF2A was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MEF2A Antibody (PB9953) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9953-5-IHC-anti-mef2a-picoband-antibody.jpgAnti-MEF2A Antibody Picoband&reg; IHC analysis of MEF2A using anti-MEF2A antibody (PB9953). <br>MEF2A was detected in a paraffin-embedded section of human meningeoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MEF2A Antibody (PB9953) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9953-6.jpgAnti-MEF2A Antibody Picoband&reg; IF analysis of MEF2A using anti-MEF2A antibody (PB9953). <br> MEF2A was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MEF2A Antibody (PB9953) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mgp-picoband-trade-antibody-pb9954-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9954-1-WB-anti-mgp-picoband-antibody.jpgAnti-MGP Antibody Picoband&reg; Western blot analysis of MGP using anti-MGP antibody (PB9954). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: JURKAT whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MGP antigen affinity purified polyclonal antibody (Catalog # PB9954) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MGP at approximately 12 kDa. The expected band size for MGP is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9954-2-IHC-anti-mgp-picoband-antibody.jpgAnti-MGP Antibody Picoband&reg; IHC analysis of MGP using anti-MGP antibody (PB9954). <br>MGP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MGP Antibody (PB9954) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-muc3-picoband-trade-antibody-pb9955-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9955-muc3-primary-antibodies-wb-testing-1.jpgAnti-MUC3/MUC3A/MUC3B Antibody Picoband&reg; Western blot analysis of MUC3 using anti-MUC3 antibody (PB9955). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SW620 whole cell lysates, <br> Lane 2: human COL320 whole cell lysates, <br> Lane 3: human CACO-2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC3 antigen affinity purified polyclonal antibody (Catalog # PB9955) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC3 at approximately 345 kDa. The expected band size for MUC3 is at 345 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-orm1-picoband-trade-antibody-pb9956-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9956-orm1-primary-antibodies-wb-testing-1.jpgAnti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband&reg; Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HCCT tissue lysates, <br> Lane 2: human HCCP tissue lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9956-2-IHC-anti-agp1-alpha-1-acid-glycoprotein-picoband-antibody.jpgAnti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband&reg; IHC analysis of ORM1 using anti-ORM1 antibody (PB9956). <br>ORM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ORM1 Antibody (PB9956) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-elafin-skalp-picoband-trade-antibody-pb9957-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9957-1-WB-anti-elafin-pi3-picoband-antibody.jpgAnti-Elafin/Skalp/PI3 Antibody Picoband&reg; Western blot analysis of Elafin/Skalp using anti-Elafin/Skalp antibody (PB9957). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Elafin/Skalp antigen affinity purified polyclonal antibody (Catalog # PB9957) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Elafin/Skalp at approximately 25 kDa. The expected band size for Elafin/Skalp is at 12 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9957-2-IHC-anti-elafin-pi3-picoband-antibody.jpgAnti-Elafin/Skalp/PI3 Antibody Picoband&reg; IHC analysis of Elafin/Skalp using anti-Elafin/Skalp antibody (PB9957). <br>Elafin/Skalp was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Elafin/Skalp Antibody (PB9957) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9957-elafin-skalp-primary-antibodies-if-testing-3.jpgAnti-Elafin/Skalp/PI3 Antibody Picoband&reg; IF analysis of Elafin/Skalp using antiElafin/Skalp antibody (PB9957). <br> Elafin/Skalp was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Elafin/Skalp Antibody (PB9957) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9957-elafin-skalp-primary-antibodies-fcm-testing-4.pngAnti-Elafin/Skalp/PI3 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-Elafin/Skalp antibody (PB9957).<br>Overlay histogram showing SiHa cells stained with PB9957 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Elafin/Skalp Antibody (PB9957,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-eaat1-picoband-trade-antibody-pb9959-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9959-eaat1-primary-antibodies-wb-testing-1.jpgAnti-EAAT1/SLC1A3 Antibody Picoband&reg; Western blot analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody (PB9959). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT1/SLC1A3 antigen affinity purified polyclonal antibody (Catalog # PB9959) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EAAT1/SLC1A3 at approximately 60 kDa. The expected band size for EAAT1/SLC1A3 is at 60 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-slc2a5-picoband-trade-antibody-pb9960-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9960-1-WB-anti-slc2a5-glut5-picoband-antibody.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg; Western blot analysis of SLC2A5 using anti-SLC2A5 antibody (PB9960). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates&#44; <br> Lane 2: K562 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC2A5 antigen affinity purified polyclonal antibody (Catalog # PB9960) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC2A5 at approximately 55KD. The expected band size for SLC2A5 is at 55KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9960-2-IHC-anti-slc2a5-glut5-picoband-antibody.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg; IHC analysis of SLC2A5 using anti-SLC2A5 antibody (PB9960). <br> SLC2A5 was detected in paraffin-embedded section of rat intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A5 Antibody (PB9960) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9960-3-IHC-anti-slc2a5-glut5-picoband-antibody.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg; IHC analysis of SLC2A5 using anti-SLC2A5 antibody (PB9960). <br> SLC2A5 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC2A5 Antibody (PB9960) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9960-4.jpgAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg; IF analysis of SLC2A5 using anti-SLC2A5 antibody (PB9960).<br> SLC2A5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SLC2A5 Antibody (PB9960) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9960-5.pngAnti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband&reg; Flow Cytometry analysis of THP-1 cells using anti-SLC2A5 antibody (PB9960). <br> Overlay histogram showing THP-1 cells stained with PB9960 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC2A5 Antibody (PB9960&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-talin-2-picoband-trade-antibody-pb9961-boster.html2026-03-25T05:22:34+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9961-1-WB-anti-talin-2-picoband-antibody.jpgAnti-Talin 2/TLN2 Antibody Picoband&reg; Western blot analysis of Talin 2 using anti-Talin 2 antibody (PB9961). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse cardiac muscle tissue lysates,<br> Lane 3: SMMC7721 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Talin 2 antigen affinity purified polyclonal antibody (Catalog # PB9961) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Talin 2 at approximately 271 kDa. The expected band size for Talin 2 is at 272 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9961-2-IHC-anti-talin-2-picoband-antibody.jpgAnti-Talin 2/TLN2 Antibody Picoband&reg; IHC analysis of Talin 2 using anti-Talin 2 antibody (PB9961). <br> Talin 2 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Talin 2 Antibody (PB9961) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9961-3-IHC-anti-talin-2-picoband-antibody.jpgAnti-Talin 2/TLN2 Antibody Picoband&reg; IHC analysis of Talin 2 using anti-Talin 2 antibody (PB9961). <br> Talin 2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Talin 2 Antibody (PB9961) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9961-4-IHC-anti-talin-2-picoband-antibody.jpgAnti-Talin 2/TLN2 Antibody Picoband&reg; IHC analysis of Talin 2 using anti-Talin 2 antibody (PB9961). <br> Talin 2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Talin 2 Antibody (PB9961) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wdr1-picoband-trade-antibody-pb9962-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9962-wdr1-primary-antibodies-wb-testing-1.jpgAnti-WDR1 Antibody Picoband&reg;Western blot analysis of WDR1 using anti-WDR1 antibody (PB9962). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U251 whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human HEL whole cell lysates,<br> Lane 4: rat small intestine tissue lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse small intestine tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR1 antigen affinity purified polyclonal antibody (PB9962) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for WDR1 at approximately 66 kDa. The expected band size for WDR1 is at 66 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9962-wdr1-primary-antibodies-ihc-testing-1.jpgAnti-WDR1 Antibody Picoband&reg;IHC analysis of WDR1 using anti-WDR1 antibody (PB9962). <br>WDR1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-WDR1 Antibody (PB9962) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9962-wdr1-primary-antibodies-if-testing-1.jpgAnti-WDR1 Antibody Picoband&reg;IF analysis of WDR1 using anti-WDR1 antibody (PB9962). <br> WDR1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-WDR1 Antibody (PB9962) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9962-wdr1-primary-antibodies-fcm-testing-1.jpgAnti-WDR1 Antibody Picoband&reg;Flow Cytometry analysis of U251 cells using anti-WDR1 antibody (PB9962). <br> Overlay histogram showing U251 cells stained with PB9962 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WDR1 Antibody (PB9962, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-he4-picoband-trade-antibody-pb9963-boster.html2026-03-24T05:05:40+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9963-1-WB-anti-he4-picoband-antibody.jpgAnti-HE4/WFDC2 Antibody Picoband&reg; Western blot analysis of HE4 using anti-HE4 antibody (PB9963). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: 22RV1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody (Catalog # PB9963) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HE4 at approximately 25 kDa. The expected band size for HE4 is at 13 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wnk1-picoband-trade-antibody-pb9964-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9964-1-WB-anti-wnk1-picoband-antibody.jpgAnti-WNK1 Antibody Picoband&reg; Western blot analysis of WNK1 using anti-WNK1 antibody (PB9964). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNK1 antigen affinity purified polyclonal antibody (Catalog # PB9964) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNK1 at approximately 250 kDa. The expected band size for WNK1 is at 251 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9964-2-IHC-anti-wnk1-picoband-antibody.jpgAnti-WNK1 Antibody Picoband&reg; IHC analysis of WNK1 using anti-WNK1 antibody (PB9964). <br> WNK1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNK1 Antibody (PB9964) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9964-3-IHC-anti-wnk1-picoband-antibody.jpgAnti-WNK1 Antibody Picoband&reg; IHC analysis of WNK1 using anti-WNK1 antibody (PB9964). <br> WNK1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNK1 Antibody (PB9964) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9964-4-IHC-anti-wnk1-picoband-antibody.jpgAnti-WNK1 Antibody Picoband&reg; IHC analysis of WNK1 using anti-WNK1 antibody (PB9964). <br> WNK1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-WNK1 Antibody (PB9964) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-xiap-picoband-trade-antibody-pb9965-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9965-xiap-primary-antibodies-wb-testing-1.jpgAnti-XIAP Antibody Picoband&reg; Western blot analysis of XIAP using anti-XIAP antibody (PB9965). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XIAP antigen affinity purified polyclonal antibody (Catalog # PB9965) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XIAP at approximately 55 kDa. The expected band size for XIAP is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-crm1-picoband-trade-antibody-pb9966-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-yap1-primary-antibodies-wb-testing-1.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; Western blot analysis of CRM1 using anti-CRM1 antibody (PB9966). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human COLO-320 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human A431 whole cell lysates, <br> Lane 6: human SGC-7901 whole cell lysates, <br> Lane 7: human 22RV1 whole cell lysates, <br> Lane 8: rat heart tissue lysates, <br> Lane 9: rat skeletal muscle tissue lysates, <br> Lane 10: rat lung tissue lysates, <br> Lane 11: rat testis tissue lysates, <br> Lane 12: mouse heart tissue lysates, <br> Lane 13: mouse skeletal muscle tissue lysates, <br> Lane 14: mouse lung tissue lysates, <br> Lane 15: mouse testis tissue lysates, <br> Lane 16: mouse ovary tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRM1 antigen affinity purified polyclonal antibody (Catalog # PB9966) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9966-2-IHC-anti-crm1-picoband-antibody.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; IHC analysis of CRM1 using anti-CRM1 antibody (PB9966). <br> CRM1 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9966-3-IHC-anti-crm1-picoband-antibody.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; IHC analysis of CRM1 using anti-CRM1 antibody (PB9966). <br> CRM1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9966-4-IHC-anti-crm1-picoband-antibody.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; IHC analysis of CRM1 using anti-CRM1 antibody (PB9966). <br> CRM1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-crm1-primary-antibodies-ihc-testing-5.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; IHC analysis of CRM1 using anti-CRM1 antibody (PB9966).<br> CRM1 was detected in frozen section of mouse intestine tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-crm1-primary-antibodies-ihc-testing-6.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; IHC analysis of CRM1 using anti-CRM1 antibody (PB9966).<br> CRM1 was detected in frozen section of rat intestine tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-crm1-primary-antibodies-ihc-testing-7.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; IHC analysis of CRM1 using anti-CRM1 antibody (PB9966).<br> CRM1 was detected in frozen section of rat testis tissue . The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-8.jpgAnti-CRM1/XPO1 Antibody Picoband&reg; IF analysis of CRM1 using anti-CRM1 antibody (PB9966).<br> CRM1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CRM1 Antibody (PB9966) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-yap1-primary-antibodies-ip-testing-1.jpgAnti-CRM1/XPO1 Antibody Picoband&reg;Immunoprecipitating (IP) CRM1 in Hela whole cell lysate.<br> Western blot analysis of CRM1 using anti-CRM1 antibody (PB9966); <br> Lane 1: Hela whole cell lysates (30ug);<br> Lane 2: Rabbit control IgG instead of anti-CRM1 antibody in Hela whole cell lysate;<br> Lane 3: anti-CRM1 antibody (2μg) + Hela whole cell lysate (500μg).<br> After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CRM1 antigen affinity purified polyclonal antibody (PB9966) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CRM1 at approximately 123 kDa. The expected band size for CRM1 is at 123 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-crm1-primary-antibodies-fc-testing-9.pngAnti-CRM1/XPO1 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-CRM1 antibody (PB9966). <br> Overlay histogram showing SiHa cells stained with PB9966 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRM1 Antibody (PB9966&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9966-crm1-primary-antibodies-fc-testing-10.pngAnti-CRM1/XPO1 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-CRM1 antibody (PB9966). <br> Overlay histogram showing U87 cells stained with PB9966 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CRM1 Antibody (PB9966&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-yap1-picoband-trade-antibody-pb9967-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-wb-testing-1_1.jpgAnti-YAP1 Antibody Picoband&reg; Western blot analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human RT4 whole cell lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: mouse RM1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (Catalog # PB9967) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-wb-testing-2.jpgAnti-YAP1 Antibody Picoband&reg; Western blot analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat RH35 whole cell lysates, <br> Lane 2: rat C6 whole cell lysates, <br> Lane 3: mosue HEPA1-6 whole cell lysates, <br> Lane 4: mouse RM1 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (Catalog # PB9967) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-ihc-testing-3.jpgAnti-YAP1 Antibody Picoband&reg; IHC analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> YAP1 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YAP1 Antibody (PB9967) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-ihc-testing-4.jpgAnti-YAP1 Antibody Picoband&reg; IHC analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> YAP1 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YAP1 Antibody (PB9967) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-ihc-testing-5.jpgAnti-YAP1 Antibody Picoband&reg; IHC analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> YAP1 was detected in a paraffin-embedded section of human diffuse large B-cell lymphoma of the intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YAP1 Antibody (PB9967) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-ihc-testing-6.jpgAnti-YAP1 Antibody Picoband&reg; IHC analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> YAP1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YAP1 Antibody (PB9967) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-ihc-testing-7.jpgAnti-YAP1 Antibody Picoband&reg; IHC analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> YAP1 was detected in a paraffin-embedded section of human thyroid tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-YAP1 Antibody (PB9967) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-if-testing-8.jpgAnti-YAP1 Antibody Picoband&reg; IF analysis of YAP1 using anti-YAP1 antibody (PB9967). <br> YAP1 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-YAP1 Antibody (PB9967) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-wb-testing-3.jpgAnti-YAP1 Antibody Picoband&reg;Western blot analysis of YAP1 using anti-YAP1 antibody (PB9967).<br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 μg of sample under reducing conditions. Lane 1: Zebrafish head tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB9967) at 0.5 μg/ml overnight at 4°C, then washed with TBS-0.1%Tween-20 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for YAP1 at approximately 70 kDa.The expected band size for YAP1 is at 54 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-if-testing-9.jpgAnti-YAP1 Antibody Picoband&reg; IF analysis of YAP1 using anti-YAP1 antibody (PB9967) and anti-Beta Tubulin antibody (M01857-3).<br> YAP1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-YAP1 Antibody (PB9967) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-ip-testing-10.jpgAnti-YAP1 Antibody Picoband&reg; Immunoprecipitating YAP1 in HepG2 whole cell lysate . Western blot analysis of YAP1 using anti-YAP1 antibody (PB9967). Lane 1: HepG2 whole cell lysates (30ug) Lane 2: Rabbit control IgG instead of anti-YAP1 antibody in HepG2 whole cell lysate. Lane 3: anti-YAP1 antibody (2μg) + HepG2 whole cell lysate (500μg) After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-YAP1 antigen affinity purified polyclonal antibody (PB9967) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for YAP1 at approximately 75 kDa. The expected band size for YAP1 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9967-yap1-primary-antibodies-fcm-testing-11.jpgAnti-YAP1 Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-YAP1 antibody (PB9967). <br> Overlay histogram showing U251 cells stained with PB9967 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YAP1 Antibody (PB9967, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zap70-picoband-trade-antibody-pb9968-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9968-zap70-primary-antibodies-wb-testing-1.jpgAnti-ZAP70 Antibody Picoband&reg; Western blot analysis of ZAP70 using anti-ZAP70 antibody (PB9968). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human CCRF-CEM whole cell lysates,<br> Lane 3: human MOLT-4 whole cell lysates,<br> Lane 4: human Raji whole cell lysates,<br> Lane 5: rat spleen tissue lysates,<br> Lane 6: rat thymus tissue lysates,<br> Lane 7: mouse spleen tissue lysates,<br> Lane 8: mouse thymus tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZAP70 antigen affinity purified polyclonal antibody (Catalog # PB9968) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZAP70 at approximately 70 kDa. The expected band size for ZAP70 is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9968-zap70-primary-antibodies-ihc-testing-2.jpgAnti-ZAP70 Antibody Picoband&reg; IHC analysis of ZAP70 using anti-ZAP70 antibody (PB9968). <br> ZAP70 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZAP70 Antibody (PB9968) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-plzf-picoband-trade-antibody-pb9969-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9969-1-WB-anti-plzf-picoband-antibody.jpgAnti-Plzf/ZBTB16 Antibody Picoband&reg; Western blot analysis of Plzf using anti-Plzf antibody (PB9969). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat ovary tissue lysates, <br> Lane 2: SKOV3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Plzf antigen affinity purified polyclonal antibody (Catalog # PB9969) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Plzf at approximately 74 kDa. The expected band size for Plzf is at 74 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9969-fphys-13-843825-g001.jpgAnti-Plzf/ZBTB16 Antibody Picoband&reg;Relative expression of specific genes in spermatogonial cells. (A) The mRNA expression of TET1 in spermatogonial cells was detected by QRT-PCR. (B) The mRNA expression of PLZF in spermatogonial cells was detected by QRT-PCR. (C) The mRNA expression of GFRα1 in spermatogonial cells was detected by QRT-PCR. (D) The mRNA expression of PRDM1 in spermatogonial cells was detected by QRT-PCR. (E) The mRNA expression of MAGE4 in spermatogonial cells was detected by QRT-PCR. (F) The expression of PLZF in TET1 overexpressed cells was detected by Western Blot. (G) Quantification of PLZF protein levels in TET1 overexpressed cells. (H) The expression of GFRα1 in TET1 overexpressed cells was detected by Western Blot. (I) Quantification of GFRα1 protein levels in TET1 overexpressed cells. p < .0001(****), p < .001(***), p < .01(**), p < .05(*).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.843825/full'>35222097</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zp1-picoband-trade-antibody-pb9970-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9970-1-WB-anti-zp1-picoband-antibody.jpgAnti-ZP1 Antibody Picoband&reg; Western blot analysis of ZP1 using anti-ZP1 antibody (PB9970). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat ovary tissue lysates, <br> Lane 2: SKOV3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZP1 antigen affinity purified polyclonal antibody (Catalog # PB9970) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZP1 at approximately 70 kDa, 100 kDa. The expected band size for ZP1 is at 70 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cacybp-antibody-rp1104-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-cacybp-primary-antibodies-wb-testing-1.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; Western blot analysis of CACYBP using anti-CACYBP antibody (RP1104). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human RT4 whole cell lysates, <br> Lane 3: human SW620 whole cell lysates, <br> Lane 4: human U251 whole cell lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: rat brain tissue lysates, <br> Lane 7: mouse liver tissue lysates, <br> Lane 8: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACYBP antigen affinity purified polyclonal antibody (Catalog # RP1104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CACYBP at approximately 26 kDa. The expected band size for CACYBP is at 26 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1104-2-IHC-anti-cacybp-siah-interacting-protein-antibody.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; IHC analysis of CACYBP using anti-CACYBP antibody (RP1104). <br> CACYBP was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CACYBP Antibody (RP1104) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-fcimb-08-00304-g002.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg;CacyBP bound to C-terminal domains of V protein. (A) Identification of protein interaction partners of V protein by yeast two-hybrid screening of a CEF cDNA library. (B–D) Co-localization of CacyBP with V, VC, and VN proteins in DF-1 cells. DF-1 cells were plated on coverslips and transfected with Flag-V, Flag-VN and Flag-VC. Forty-eight hours after transfection, the cells were stained with mouse anti-FLAG and rabbit anti-CacyBP antibodies, which was followed by staining with donkey anti-rabbit Alexa Fluor ® 488 (green) and goat anti-mouse Alexa Fluor ® ; 594 (Red) as secondary antibodies. The nucleus was subsequently stained with Hoechst 33342, and the images were captured using an ANDOR Revolution WD confocal microscope. Pearson's coefficient were analysis by Imaris (Microscopy Image Analysis Software, Bitplane, Switzerland), and Pearson's coefficient >0.5 were considered to be probable co-localization. (E–G) HEK-293T cells in 60 mm cell culture dishes were co-transfected with the pCAGEN-Flag-V (or Flag-VN or Flag-VC) and pCMV-HA-CacyBP expression plasmids. Transfected cells were harvested and lysed 48 h after transfection, and the experiment was conducted according to the manufacturer's instructions for the Co-Immunoprecipitation Kit. After washing, immunoprecipitated proteins were identified and analyzed by western blotting using anti-HA or anti-FLAG antibodies.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2018.00304/full'>30234028</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-fcimb-08-00304-g003.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg;Overexpression of CacyBP in DF-1 cells arrested viral replication and enhanced apoptosis. DF-1 cells were transfected with mock and/or control and CacyBP/SIP and incubated for 48 h. Mock, treated with transfection reagent; control, transfected with pCMV-3HA; CacyBP, transfected with pCMV-3HA-CacyBP/SIP. (A) Endogenous and exogenous CacyBP/SIP was detected by western blotting. (B) Expression of the 3HA-CacyBP protein in DF-1 cells was detected using immunofluorescence. (C) Replication kinetics of NDV RNAs from DF-1 cells. Both cell lysates and supernatants were collected 48 h after transfection (at 24 h after transfection, 1 MOI of F48E9 NDV was inoculated in three groups). Q-PCR was used to measure viral RNA replication. (D) Viral plaque formation tests were further used to measure the number of virus particles in supernatants. (E) Flow cytometry was used to analyze cell apoptosis. DF-1 cells were transfected with mock and/or control and CacyBP/SIP and incubated for 48 h, an annexin V assay was followed by flow cytometry to monitor for percentage of cells undergoing early apoptosis (bottom right quadrant) and late apoptosis(upper right quadrant).Y-axis is PI signal;X-asis is annexin V-FITC signal, right graph data were the percentage of total apoptosis (bottom and upper right quadrant)and data from three independent experiments. (F) The transfected cell lysate was analyzed by western blotting with the indicated apoptosis-related antibody. (G) Q-PCR was used to analyze the expression of apoptosis-related markers and (H) immune-associated markers 24 h after transfection with pCMV-HA or pCMV-HA-CacyBP. Data shown in (C,D) are mean ± SD of four independent experiments in (E,G,H) are mean ± SD of three independent expriments. * P < 0.05; ** P < 0.01; *** P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2018.00304/full'>30234028</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-fcimb-08-00304-g005.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg;V protein inhibited apoptosis by downregulating CacyBP in DF-1 cells. DF-1 cells were transfected with the control (pCAGEN-Flag), pCAGEN-Flag-VC, and pCAGEN-Flag-V. (A) The transfected cells were washed and harvested 48 h after transfection, and flow cytometry was used to analyze cell apoptosis. (B) Twenty-four and forty-eight hours after transfection, the mRNA levels of CacyBP were measured by Q-PCR. (C) Protein expression of CacyBP was measured in DF-1 cells. The cells were transfected with pCAGEN-Flag (control) and pCAGEN-Flag-V (1, 2, and 4 μg), and after 48 h, the cells were harvested and analyzed by western blotting. Data shown in (A,B) are mean ± SD of three independent experiments. * P < 0.05; ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2018.00304/full'>30234028</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-fcimb-08-00304-g004.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg;Knockdown of CacyBP facilitated viral replication and arrested apoptosis in DF-1 cells. DF-1 cells were transfected with mock and/or NC and si-CacyBP/SIP and incubated for 36 h. Mock, treated with transfection regent; NC, transfected with si-NC; si183/326/644, separately transfected with those siRNAs targets CacyBP/SIP. (A) Endogenous CacyBP/SIP was detected by western blotting. (B) DF-1 cells were co-transfected with si-CacyBP (326) and pCMV-HA-CacyBP. Thirty-six hours after transfection, protein expression of HA-CacyBP was detected by anti-HA antibody through immunofluorescence in DF-1 cells. (C) Replication kinetics of NDV RNAs from the mock, NC, and siRNA (326) groups of DF-1 cells; 1 MOI of F48E9 NDV were inoculated into the three groups of cells 24 h after transfection. Q-PCR was used to measure viral RNA replication 24 hpi. (D) Viral plaque formation tests were further used to measure the number of viruses in the supernatants. (E) Three cell cultures were prepared and transfected with mock, NC and siRNA (326), and then, the cells were washed and harvested, an annexin V assay was followed by flow cytometry to monitor for percentage of cells undergoing early apoptosis (bottom right quadrant) and late apoptosis(upper right quadrant). Y-axis is PI signal; X-asis is annexin V-FITC signal. Right graph data were the percentage of total apoptosis (bottom and upper right quadrant)and data from three independent experiments. (F–H) Q-PCR was used to analyze the expression of apoptosis-related markers and immune-associated markers 24 h after transfection with NC and si-CacyBP/SIP. (G) The transfected cell lysate was analyzed by western blotting with the indicated apoptosis-related antibody. Data shown in (C,D) are mean ± SD of four independent experiments, in (E,G,H) are mean ± SD of three independent expriments. * P < 0.05 and ** P < 0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2018.00304/full'>30234028</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-fcimb-14-1446305-g003.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg;(A) H&E and Sirius red staining revealed severe pulmonary fibrosis in the COVID-19 and PQ groups. (B, C) IHC demonstrate that CACYBP is located in both the cytoplasm and the nucleus, with higher abundance observed in the COVID-19 comparison group than that in the PQ comparison group (Scale: 50× for low magnification, 200× for high magnification; *: P<0.05; ns: no significance). <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1446305/full'>39301288</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1104-3-IHC-anti-cacybp-siah-interacting-protein-antibody.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; IHC analysis of CACYBP using anti-CACYBP antibody (RP1104). <br> CACYBP was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CACYBP Antibody (RP1104) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1104-4-IHC-anti-cacybp-siah-interacting-protein-antibody.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; IHC analysis of CACYBP using anti-CACYBP antibody (RP1104). <br> CACYBP was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CACYBP Antibody (RP1104) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-5.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; ICC analysis of CACYBP using anti-CACYBP antibody (RP1104). <br> CACYBP was detected in immunocytochemical section of MCF-7 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-CACYBP Antibody (RP1104) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1104-cacybp-primary-antibodies-ip-testing-6.jpgAnti-SIAH Interacting Protein/CACYBP Antibody Picoband&reg; Immunoprecipitating CACYBP in U251 whole cell lysate.<br>Western blot analysis of CACYBP using anti-CACYBP antibody (RP1104). <br>Lane 1: U251 whole cell lysates (30ug),<br>Lane 2: Rabbit control IgG instead of anti-CACYBP antibody in U251 whole cell lysate,<br>Lane 3: anti-CACYBP antibody (2μg) + U251 whole cell lysate (500μg).<br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CACYBP antigen affinity purified polyclonal antibody (RP1104) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CACYBP at approximately 27 kDa. The expected band size for CACYBP is at 27 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd33-antibody-rp1105-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1105-2-IHC-anti-cd33-siglec-3-antibody.jpgAnti-CD33 Antibody Picoband&reg;CD33 was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody (Catalog # RP1105) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1105-1-WB-anti-cd33-siglec-3-antibody.jpgAnti-CD33 Antibody Picoband&reg;Western blot analysis of CD33 expression in mosue lung extract (lane 1). CD33 at 45KD was detected using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody (Catalog # RP1105) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lfa3-antibody-rp1106-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1106-1-WB-anti-lfa3-antibody.jpgAnti-LFA3/CD58 Antibody Picoband&reg;Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35KD was detected using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1106-2-IHC-anti-lfa3-antibody.jpgAnti-LFA3/CD58 Antibody Picoband&reg;LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody (Catalog # RP1106) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1106-lfa3-primary-antibodies-fc-testing-3.pngAnti-LFA3/CD58 Antibody Picoband&reg; Flow Cytometry analysis of Hela cells using anti-LFA3 antibody (RP1106).<br> Overlay histogram showing Hela cells stained with RP1106 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LFA3 Antibody (RP1106&#44;1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cofilin-2-antibody-rp1107-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1107-cofilin_2-primary-antibodies-wb-testing-1.jpgAnti-Cofilin 2/CFL2 Antibody Picoband&reg; Western blot analysis of Cofilin 2 using anti-Cofilin 2 antibody (RP1107). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates,<br> Lane 2: human HUVEC whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat skeletal muscle tissue lysates,<br> Lane 6: rat RH35 whole cell lysates,<br> Lane 7: rat liver tissue lysates,<br> Lane 8: mouse skeletal muscle tissue lysates,<br> Lane 9: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cofilin 2 antigen affinity purified polyclonal antibody (Catalog # RP1107) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cofilin 2 at approximately 19 kDa. The expected band size for Cofilin 2 is at 19 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1107-cofilin_2-primary-antibodies-if-testing-2.jpgAnti-Cofilin 2/CFL2 Antibody Picoband&reg; IF analysis of Cofilin 2 using anti-Cofilin 2 antibody (RP1107). <br> Cofilin 2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cofilin 2 Antibody (RP1107) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1107-cofilin_2-primary-antibodies-fcm-testing-3.jpgAnti-Cofilin 2/CFL2 Antibody Picoband&reg; Flow Cytometry analysis of Raji cells using anti-Cofilin 2 antibody (RP1107). <br> Overlay histogram showing Raji cells stained with RP1107 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cofilin 2 Antibody (RP1107, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vip-antibody-rp1108-boster.html2026-03-24T05:05:41+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-vip-primary-antibodies-ihc-testing-1.jpgAnti-VIP peptides VIP Antibody IHC analysis of VIP using anti-VIP antibody (RP1108). <br> VIP was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1108-2-IHC-anti-vip-antibody.jpgAnti-VIP peptides VIP Antibody IHC analysis of VIP using anti-VIP antibody (RP1108). <br> VIP was detected in a paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-12886_2024_3309_fig5_html.pngAnti-VIP peptides VIP AntibodyImmunohistochemical performance of retinal VIP protein in guinea pigs (DAB, X400). a The NC group. b The FDM group. c The FDM + atropine group. The VIP protein positive expression was brown and yellow <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12886-024-03309-9'>38279089</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-12886_2024_3309_fig6_html.pngAnti-VIP peptides VIP AntibodyIn situ hybridization performance of retinal VIP mRNA in guinea pigs (DAB, X400). a The NC group. b The FDM group. c The FDM + atropine group. The VIP mRNA positive expression was brown and yellow <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12886-024-03309-9'>38279089</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-12886_2024_3309_fig7_html.pngAnti-VIP peptides VIP AntibodyProtein and mRNA expressions of retinal VIP in guinea pig (mean ± SD). Compared with the NC group, the mean optical density of retinal VIP protein and VIP mRNA positive cells in the FDM group were significantly up-regulated ( P < 0.01). In contrast, those in the FDM + AT group did not change significantly ( P > 0.05) <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12886-024-03309-9'>38279089</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-vip-primary-antibodies-ihc-testing-3.jpgAnti-VIP peptides VIP Antibody IHC analysis of VIP using anti-VIP antibody (RP1108). <br> VIP was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-4-ihc-anti-vip-antibody.jpgAnti-VIP peptides VIP Antibody IHC analysis of VIP using anti-VIP antibody (RP1108). <br> VIP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-vip-primary-antibodies-ihc-testing-5_1.jpgAnti-VIP peptides VIP Antibody IHC analysis of VIP using anti-VIP antibody (RP1108). <br> VIP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1108-vip-primary-antibodies-if-testing-6.jpgAnti-VIP peptides VIP Antibody IF analysis of VIP using anti-VIP antibody (RP1108). <br> VIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-VIP Antibody (RP1108) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sr1-antibody-rp1110-boster.html2026-03-24T05:05:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1110-1-WB-anti-sr1-antibody.jpgAnti-SR1/SRI Antibody Picoband&reg;Western blot analysis of SRI expression in rat liver extract (lane 1)&#44; rat brain extract (lane 2) and SMMC7721 whole cell lysates (lane 3). SRI at 26KD was detected using rabbit anti-SRI Antigen Affinity purified polyclonal antibody (Catalog # RP1110) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-caldesmon-picoband-trade-antibody-pb9922-boster.html2026-03-24T05:05:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9922-caldesmon-primary-antibodies-wb-testing-1.jpgAnti-Caldesmon/CALD1 Antibody Picoband&reg; Western blot analysis of Caldesmon using anti-Caldesmon antibody (PB9922). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U20S whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human Hela whole cell lysates, <br> Lane 5: human CACO-2 whole cell lysates, <br> Lane 6: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caldesmon antigen affinity purified polyclonal antibody (Catalog # PB9922) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caldesmon at approximately 80 kDa. The expected band size for Caldesmon is at 93 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9922-2_1-IHC-anti-caldesmon-picoband-antibody.jpgAnti-Caldesmon/CALD1 Antibody Picoband&reg; IHC analysis of Caldesmon using anti-Caldesmon antibody (PB9922). <br> Caldesmon was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Caldesmon Antibody (PB9922) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9922-3_1-IHC-anti-caldesmon-picoband-antibody.jpgAnti-Caldesmon/CALD1 Antibody Picoband&reg; IHC analysis of Caldesmon using anti-Caldesmon antibody (PB9922). <br> Caldesmon was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Caldesmon Antibody (PB9922) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9922-4_1-IHC-anti-caldesmon-picoband-antibody.jpgAnti-Caldesmon/CALD1 Antibody Picoband&reg; IHC analysis of Caldesmon using anti-Caldesmon antibody (PB9922). <br> Caldesmon was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Caldesmon Antibody (PB9922) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9922-caldesmon-primary-antibodies-if-testing-5.jpgAnti-Caldesmon/CALD1 Antibody Picoband&reg; IF analysis of Caldesmon using anti-Caldesmon antibody (PB9922). <br> Caldesmon was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Caldesmon Antibody (PB9922) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9922-caldesmon-primary-antibodies-fcm-testing-6.jpgAnti-Caldesmon/CALD1 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-Caldesmon antibody (PB9922). <br> Overlay histogram showing U87 cells stained with PB9922 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caldesmon Antibody (PB9922, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/broad-spectrum-protease-inhibitor-cocktail-edta-free-100x-ar1182-1-boster.html2026-03-25T05:22:36+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1182-1_2.jpgBroad Spectrum Protease Inhibitor Cocktail (EDTA free) (100x)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/r/ar1182-1.pngBroad Spectrum Protease Inhibitor Cocktail (EDTA free) (100x)Protease activity for extracted protein from mouse tissue while using different protease inhibitor cocktails https://www.bosterbio.com/picokine-elisa-kits/human-ldlr-picokine-trade-elisa-kit-ek1542-boster.html2026-03-24T05:05:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1542_1.pngHuman LDLR ELISA Kit PicoKine®Human LDLR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ldlr-picokine-trade-elisa-kit-ek1543-boster.html2026-03-24T05:05:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1543_1.pngMouse LDLR / OLR1 ELISA Kit PicoKine®Mouse LDLR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-spint1-hai-1-picokine-trade-elisa-kit-ek0771-boster.html2026-03-24T05:05:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0771_1.pngHuman SPINT1/HAI-1 ELISA Kit PicoKine®Human SPINT1/HAI-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-spint1-hai-1-picokine-trade-elisa-kit-ek0772-boster.html2026-03-24T05:05:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0772_1.pngMouse SPINT1/HAI-1 ELISA Kit PicoKine®Mouse SPINT1/HAI-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-bcam-picokine-trade-elisa-kit-ek1546-boster.html2026-03-24T05:05:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1546_1.pngHuman BCAM/Cd239 ELISA Kit PicoKine®Human BCAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-bcam-picokine-trade-elisa-kit-ek1547-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1547.pngMouse BCAM/Cd239 ELISA Kit PicoKine®Mouse BCAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-oncomodulin-picokine-trade-elisa-kit-ek1545-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1545_1.pngHuman Oncomodulin ELISA Kit PicoKine®Human Oncomodulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-saa-saa1-picokine-trade-elisa-kit-ek1544-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1544.jpgHuman SAA/SAA1 ELISA Kit PicoKine®Human SAA/SAA1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sfrp5-picokine-trade-elisa-kit-ek1472-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1472_1.pngHuman SFRP5/Sarp3 ELISA Kit PicoKine®Human SFRP5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ca125-muc16-picokine-trade-elisa-kit-ek1471-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1471.jpgHuman CA125/MUC16 ELISA Kit PicoKine®Human CA125/MUC16 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-mmp-9-picokine-trade-elisa-kit-ek1463-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1463.jpgRat MMP-9 ELISA Kit PicoKine®Rat MMP-9 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-marapsin-pancresin-picokine-trade-elisa-kit-ek1479-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1479.jpgHuman Marapsin/Pancresin ELISA Kit PicoKine®Human Marapsin/Pancresin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-marapsin-pancresin-picokine-trade-elisa-kit-ek1481-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1481.jpgMouse Marapsin/Pancresin ELISA Kit PicoKine®Mouse Marapsin/Pancresin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-fcrn-fcgrt-picokine-trade-elisa-kit-ek1454-boster.html2026-03-24T05:05:43+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1454.jpgMouse FCRN/FCGRT ELISA Kit PicoKine®Mouse FCRN/FCGRT PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fabp4-picokine-trade-elisa-kit-ek1459-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1459_2.pngHuman FABP4/A Fabp ELISA Kit PicoKine®Human FABP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-fabp4-picokine-trade-elisa-kit-ek1458-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1458_1.pngMouse FABP4/A Fabp ELISA Kit PicoKine®Mouse FABP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-he4-picokine-trade-elisa-kit-ek1469-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1469.pngHuman HE4 ELISA Kit PicoKine®Human HE4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hemopexin-picokine-trade-elisa-kit-ek1466-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1466.jpgHuman Hemopexin ELISA Kit PicoKine®Human Hemopexin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-ghr-picokine-trade-elisa-kit-ek1467-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1467_1.pngHuman GHR/Growth Hormone R ELISA Kit PicoKine®Human GHR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fibulin-3-efemp1-picokine-trade-elisa-kit-ek1468-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1468.jpgHuman Fibulin-3/EFEMP1 ELISA Kit PicoKine®Human Fibulin-3/EFEMP1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd28-picokine-trade-elisa-kit-ek1429-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1429_1.pngHuman CD28 ELISA Kit PicoKine®Human CD28 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tpor-mpl-picokine-trade-elisa-kit-ek1436-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1436_1.pngMouse TPOR/MPL/Thrombopoietin R ELISA Kit PicoKine®Mouse TPOR/MPL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-galectin-1-picokine-trade-elisa-kit-ek0762-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0762_2.pngHuman Galectin-1 ELISA Kit PicoKine®Human Galectin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-r-spondin-1-picokine-trade-elisa-kit-ek1516-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1516_2.pngHuman R-Spondin-1 ELISA Kit PicoKine®Human R-Spondin-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-r-spondin-3-picokine-trade-elisa-kit-ek1512-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1512_1.pngHuman R-Spondin-3 ELISA Kit PicoKine®Human R-Spondin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-tnfrsf25-dr3-picokine-trade-elisa-kit-ek1515-boster.html2026-03-24T05:05:44+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1515_1.pngMouse TNFRSF25/DR3 ELISA Kit PicoKine®Mouse TNFRSF25/DR3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-siglec-7-cd328-picokine-trade-elisa-kit-ek1508-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1508.jpgHuman Siglec-7/CD328 ELISA Kit PicoKine®Human Siglec-7/CD328 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-tafi-cpb2-picokine-trade-elisa-kit-ek1502-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1502_1.pngHuman TAFI/CPB2/Carboxypeptidase B2 ELISA Kit PicoKine®Human TAFI/CPB2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-gdf5-picokine-trade-elisa-kit-ek1505-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1505.jpgMouse GDF5/Bmp 14 ELISA Kit PicoKine®Mouse GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-gdf5-picokine-trade-elisa-kit-ek1504-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504_1.pngRat GDF5/Bmp 14 ELISA Kit PicoKine®Rat GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-lrig3-picokine-trade-elisa-kit-ek1503-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1503_1.pngHuman LRIG3 ELISA Kit PicoKine®Human LRIG3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sphk1-picokine-trade-elisa-kit-ek1501-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1501.jpgHuman SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®Human SPHK1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-edil3-picokine-trade-elisa-kit-ek1500-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1500_3.pngHuman EDIL3 ELISA Kit PicoKine®Human EDIL3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-persephin-picokine-trade-elisa-kit-ek1509-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1509.jpgHuman Persephin ELISA Kit PicoKine®Human Persephin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-persephin-picokine-trade-elisa-kit-ek1499-boster.html2026-03-24T05:05:45+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1499.jpgMouse Persephin ELISA Kit PicoKine®Mouse Persephin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fetuin-b-picokine-trade-elisa-kit-ek1498-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1498_1.pngHuman Fetuin B/FETUB ELISA Kit PicoKine®Human Fetuin B PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-dtk-tyro3-picokine-trade-elisa-kit-ek1496-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1496.jpgHuman DTK/TYRO3 ELISA Kit PicoKine®Human DTK/TYRO3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-r-spondin-4-picokine-trade-elisa-kit-ek1494-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1494_1.pngHuman R-Spondin-4 ELISA Kit PicoKine®Human R-Spondin-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-adam15-picokine-trade-elisa-kit-ek1484-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1484_1.pngHuman ADAM15 ELISA Kit PicoKine®Human ADAM15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-alk-1-acvrl1-picokine-trade-elisa-kit-ek1518-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1518.jpgHuman ALK-1/ACVRL1 ELISA Kit PicoKine®Human ALK-1/ACVRL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-alk-1-acvrl1-picokine-trade-elisa-kit-ek1519-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1519.jpgMouse ALK-1/ACVRL1 ELISA Kit PicoKine®Mouse ALK-1/ACVRL1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-nope-igdcc4-picokine-trade-elisa-kit-ek1520-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1520.jpgMouse NOPE/IGDCC4 ELISA Kit PicoKine®Mouse NOPE/IGDCC4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-treml1-picokine-trade-elisa-kit-ek1521-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1521_2.pngHuman TREML1/Tlt 1 ELISA Kit PicoKine®Human TREML1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-treml1-picokine-trade-elisa-kit-ek1522-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1522_2.pngMouse TREML1/Tlt 1 ELISA Kit PicoKine®Mouse TREML1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-wif1-picokine-trade-elisa-kit-ek1524-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1524.jpgHuman WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®Human WIF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-wif1-picokine-trade-elisa-kit-ek1523-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1523.jpgMouse WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®Mouse WIF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-r-spondin-3-picokine-trade-elisa-kit-ek1525-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1525_1.pngMouse R-Spondin-3 ELISA Kit PicoKine®Mouse R-Spondin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-cd48-picokine-trade-elisa-kit-ek1527-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1527_1.pngHuman CD48/Slamf2 ELISA Kit PicoKine®Human CD48 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-icam5-picokine-trade-elisa-kit-ek1529-boster.html2026-03-24T05:05:46+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1529_1.pngHuman ICAM5 ELISA Kit PicoKine®Human ICAM5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-pan-trypsin-picokine-trade-elisa-kit-ek1531-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1531_1.pngHuman Pan Trypsin ELISA Kit PicoKine®Human Pan Trypsin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-grem2-picokine-trade-elisa-kit-ek1533-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1533_1.pngHuman GREM2/Prdc ELISA Kit PicoKine®Human GREM2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-grem2-picokine-trade-elisa-kit-ek1532-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1532_1.pngMouse GREM2/Prdc ELISA Kit PicoKine®Mouse GREM2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-nectin-4-pvrl4-picokine-trade-elisa-kit-ek1536-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1536_1.pngHuman Nectin-4/PVRL4 ELISA Kit PicoKine®Human Nectin-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-kremen-1-picokine-trade-elisa-kit-ek1538-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1538.jpgMouse Kremen-1 ELISA Kit PicoKine®Mouse Kremen-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hai-2-spint2-picokine-trade-elisa-kit-ek0773-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0773_1.pngHuman HAI-2/SPINT2 ELISA Kit PicoKine®Human HAI-2/SPINT2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-hai-2-spint2-picokine-trade-elisa-kit-ek0774-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0774_1.pngMouse HAI-2/SPINT2 ELISA Kit PicoKine®Mouse HAI-2/SPINT2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-agp1-alpha-1-acid-glycoprotein-picokine-trade-elisa-kit-ek1486-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1486.jpgHuman AGP1/alpha 1 acid glycoprotein ELISA Kit PicoKine®Human AGP1/alpha 1 acid glycoprotein PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abcb10-picoband-trade-antibody-pb9971-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9971-1-WB-anti-abcb10-abc-me-picoband-antibody.jpgAnti-ABCB10 Antibody Picoband&reg; Western blot analysis of ABCB10 using anti-ABCB10 antibody (PB9971). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat cardiac muscle tissue lysates, <br> Lane 2: COLO320 whole cell lysates, <br> Lane 3: 22RV1 whole cell lysates, <br> Lane 4: PANC whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB10 antigen affinity purified polyclonal antibody (Catalog # PB9971) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB10 at approximately 79 kDa, 65 kDa. The expected band size for ABCB10 is at 79 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abr-picoband-trade-antibody-pb9972-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9972-1-WB-anti-abr-picoband-antibody.jpgAnti-ABR Antibody Picoband&reg; Western blot analysis of ABR using anti-ABR antibody (PB9972). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABR antigen affinity purified polyclonal antibody (Catalog # PB9972) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABR at approximately 98 kDa. The expected band size for ABR is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9972-2-IHC-anti-abr-picoband-antibody.jpgAnti-ABR Antibody Picoband&reg; IHC analysis of ABR using anti-ABR antibody (PB9972). <br> ABR was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ABR Antibody (PB9972) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9972-3-IHC-anti-abr-picoband-antibody.jpgAnti-ABR Antibody Picoband&reg; IHC analysis of ABR using anti-ABR antibody (PB9972). <br> ABR was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ABR Antibody (PB9972) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9972-4-IHC-anti-abr-picoband-antibody.jpgAnti-ABR Antibody Picoband&reg; IHC analysis of ABR using anti-ABR antibody (PB9972). <br> ABR was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ABR Antibody (PB9972) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aconitase-2-picoband-trade-antibody-pb9973-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9973-aco2-primary-antibodies-wb-testing-1.jpgAnti-Aconitase 2/ACO2 Antibody Picoband&reg; Western blot analysis of ACO2 using anti-ACO2 antibody (PB9973). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human U87 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat skeletal muscle tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse skeletal muscle tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACO2 antigen affinity purified polyclonal antibody (Catalog # PB9973) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACO2 at approximately 85 kDa. The expected band size for ACO2 is at 85 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-actinin-4-picoband-trade-antibody-pb9974-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-wb-testing-1.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; Western blot analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody (PB9974). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: huamn HT1080 whole cell lysates, <br> Lane 5: human placenta tissue lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: human U251 whole cell lysates, <br> Lane 8: human RT-4 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Actinin 4 antigen affinity purified polyclonal antibody (Catalog # PB9974) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Actinin 4 at approximately 105 kDa. The expected band size for Alpha Actinin 4 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-wb-testing-2.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; Western blot analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody (PB9974). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue lysates, <br> Lane 2: rai liver tissue lysates, <br> Lane 3: rat lung tissue lysates, <br> Lane 4: rat RH35 whole cell lysates, <br> Lane 5: mouse sketetal muscle tissue lysates, <br> Lane 6: mouse liver tissue lysates, <br> Lane 7: mouse lung tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Actinin 4 antigen affinity purified polyclonal antibody (Catalog # PB9974) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Actinin 4 at approximately 105 kDa. The expected band size for Alpha Actinin 4 is at 105 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-fcm-testing-6.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-Alpha Actinin 4 antibody (PB9974). <br>Overlay histogram showing U87 cells stained with PB9974 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Actinin 4 Antibody (PB9974, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-ihc-testing-3.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; IHC analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody (PB9974). <br> Alpha Actinin 4 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Actinin 4 Antibody (PB9974) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-fcm-testing-7.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; Flow Cytometry analysis of HEPA1-6 cells using anti-Alpha Actinin 4 antibody (PB9974). <br>Overlay histogram showing HEPA1-6 cells stained with PB9974 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Actinin 4 Antibody (PB9974, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-ihc-testing-4.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; IHC analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody (PB9974). <br> Alpha Actinin 4 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Actinin 4 Antibody (PB9974) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-fcm-testing-8.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; Flow Cytometry analysis of C6 cells using anti-Alpha Actinin 4 antibody (PB9974). <br>Overlay histogram showing C6 cells stained with PB9974 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Actinin 4 Antibody (PB9974, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9974-actn4-primary-antibodies-if-testing-5.jpgAnti-Alpha Actinin 4/ACTN4 Antibody Picoband&reg; IF analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody (PB9974). <br> Alpha Actinin 4 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Alpha Actinin 4 Antibody (PB9974) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acvr2b-picoband-trade-antibody-pb9975-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9975-1_1-WB-anti-acvr2b-actr-iib-picoband-antibody.jpgAnti-Activin Receptor Type IIB/ACVR2B Antibody Picoband&reg; Western blot analysis of ACVR2B using anti-ACVR2B antibody (PB9975). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue lysates, <br> Lane 2: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2B antigen affinity purified polyclonal antibody (Catalog # PB9975) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR2B at approximately 55 kDa. The expected band size for ACVR2B is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adar1-picoband-trade-antibody-pb9976-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9976-1_1.jpgAnti-ADAR1 Antibody Picoband&reg; Western blot analysis of ADAR1 using anti-ADAR1 antibody (PB9976). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates&#44; <br> Lane 2: A549 whole cell lysates <br> Lane 3: MCF-7 whole cell lysates <br> Lane 4: HEPG2 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR1 antigen affinity purified polyclonal antibody (Catalog # PB9976) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAR1 at approximately 110KD&#44; 150KD. The expected band size for ADAR1 is at 136KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9976-2_1-IHC-anti-adar1-picoband-antibody.jpgAnti-ADAR1 Antibody Picoband&reg; IHC analysis of ADAR1 using anti-ADAR1 antibody (PB9976). <br> ADAR1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADAR1 Antibody (PB9976) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aebp2-picoband-trade-antibody-pb9977-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9977-aebp2-primary-antibodies-wb-testing-1.jpgAnti-AEBP2 Antibody Picoband&reg; Western blot analysis of AEBP2 using anti-AEBP2 antibody (PB9977). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human Jurkat whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human HL-60 whole cell lysates, <br> Lane 5: human RT4 whole cell lysates, <br> Lane 6: human SiHa whole cell lysates, <br> Lane 7: human CACO-2 whole cell lysates, <br> Lane 8: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AEBP2 antigen affinity purified polyclonal antibody (Catalog # PB9977) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AEBP2 at approximately 54 kDa. The expected band size for AEBP2 is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9977-2.jpgAnti-AEBP2 Antibody Picoband&reg; IHC analysis of AEBP2 using anti-AEBP2 antibody (PB9977).<br> AEBP2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AEBP2 Antibody (PB9977) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9977-3.jpgAnti-AEBP2 Antibody Picoband&reg; IHC analysis of AEBP2 using anti-AEBP2 antibody (PB9977).<br> AEBP2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AEBP2 Antibody (PB9977) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9977-4.jpgAnti-AEBP2 Antibody Picoband&reg; IHC analysis of AEBP2 using anti-AEBP2 antibody (PB9977).<br> AEBP2 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AEBP2 Antibody (PB9977) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9977-5.jpgAnti-AEBP2 Antibody Picoband&reg; IF analysis of AEBP2 using anti-AEBP2 antibody (PB9977). <br> AEBP2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AEBP2 Antibody (PB9977) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9977-6.jpgAnti-AEBP2 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-AEBP2 antibody (PB9977).<br>Overlay histogram showing SiHa cells stained with PB9977 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AEBP2 Antibody (PB9977,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ago2-eif2c2-picoband-trade-antibody-pb9978-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9978-ago2-primary-antibodies-wb-testing-1.jpgAnti-Ago2/eIF2C2 Antibody Picoband&reg; Western blot analysis of Ago2/eIF2C2 using anti-Ago2/eIF2C2 antibody (PB9978). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human K562 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat lung tissue lysates,<br> Lane 6: rat pancreas tissue lysates,<br> Lane 7: mouse pancreas tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ago2/eIF2C2 antigen affinity purified polyclonal antibody (Catalog # PB9978) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ago2/eIF2C2 at approximately 97 kDa. The expected band size for Ago2/eIF2C2 is at 97 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-argonaute-4-picoband-trade-antibody-pb9979-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9979-ago4-primary-antibodies-wb-testing-1.jpgAnti-Argonaute 4/AGO4 Antibody Picoband&reg;Western blot analysis of AGO4 using anti-AGO4 antibody (PB9979). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGO4 antigen affinity purified polyclonal antibody (PB9979) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AGO4 at approximately 100 kDa. The expected band size for AGO4 is at 97 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9979-ago4-primary-antibodies-ihc-testing-1.jpgAnti-Argonaute 4/AGO4 Antibody Picoband&reg;IHC analysis of AGO4 using anti-AGO4 antibody (PB9979). <br>AGO4 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AGO4 Antibody (PB9979) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aire-picoband-trade-antibody-pb9980-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9980-aire-primary-antibodies-wb-testing-1_1.jpgAnti-AIRE Antibody Picoband&reg; Western blot analysis of AIRE using anti-AIRE antibody (PB9980). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U937 whole cell lysates, <br> Lane 2: human RT4 whole cell lysates, <br> Lane 3: human Jurkat whole cell lysates, <br> Lane 4: human K562 whole cell lysates, <br> Lane 5: rat spleen tissue lysates, <br> Lane 6: rat thymus tissue lysates, <br> Lane 7: mouse spleen tissue lysates, <br> Lane 8: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AIRE antigen affinity purified polyclonal antibody (Catalog # PB9980) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AIRE at approximately 58-68 kDa. The expected band size for AIRE is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9980-aire-primary-antibodies-ihc-testing-2_1.jpgAnti-AIRE Antibody Picoband&reg; IHC analysis of AIRE using anti-AIRE antibody (PB9980). <br> AIRE was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AIRE Antibody (PB9980) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9980-aire-primary-antibodies-if-testing-3.jpgAnti-AIRE Antibody Picoband&reg; IF analysis of AIRE using anti-AIRE antibody (PB9980). <br> AIRE was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AIRE Antibody (PB9980) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9980-aire-primary-antibodies-fcm-testing-4.jpgAnti-AIRE Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-AIRE antibody (PB9980). <br> Overlay histogram showing K562 cells stained with PB9980 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AIRE Antibody (PB9980, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh1a2-picoband-trade-antibody-pb9981-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9981-aldh1a2-primary-antibodies-wb-testing-1.jpgAnti-ALDH1A2 Antibody Picoband&reg;Western blot analysis of ALDH1A2 using anti-ALDH1A2 antibody (PB9981). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: human 293T whole cell lysates,<br> Lane 3: monkey kidney tissue lysates,<br> Lane 4: human A549 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1A2 antigen affinity purified polyclonal antibody (Catalog # PB9981) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1A2 at approximately 57 kDa. The expected band size for ALDH1A2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9981-aldh1a2-primary-antibodies-if-testing-1.jpgAnti-ALDH1A2 Antibody Picoband&reg;IF analysis of ALDH1A2 using anti-ALDH1A2 antibody (PB9981). <br> ALDH1A2 was detected in immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ALDH1A2 Antibody (PB9981) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh3a2-picoband-trade-antibody-pb9982-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9982-aldh3a2-primary-antibodies-wb-testing-1.jpgAnti-ALDH3A2 Antibody Picoband&reg; Western blot analysis of ALDH3A2 using anti-ALDH3A2 antibody (PB9982). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: rat testis tissue lysates, <br> Lane 3: human Hela whole cell lysates, <br> Lane 4: human A431 whole cell lysates, <br> Lane 5: human MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A2 antigen affinity purified polyclonal antibody (Catalog # PB9982) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A2 at approximately 55 kDa. The expected band size for ALDH3A2 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9982-2-IHC-anti-aldh3a2-faldh-picoband-antibody.jpgAnti-ALDH3A2 Antibody Picoband&reg; IHC analysis of ALDH3A2 using anti-ALDH3A2 antibody (PB9982). <br> ALDH3A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ALDH3A2 Antibody (PB9982) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-amacr-picoband-trade-antibody-pb9983-boster.html2026-03-24T05:05:47+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9983-amacr-primary-antibodies-wb-testing-1.jpgAnti-AMACR Antibody Picoband&reg; Western blot analysis of AMACR using anti-AMACR antibody (PB9983). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human LNCAP whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human Hela whole cell lysates,<br> Lane 5: rat kidney tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse kidney tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMACR antigen affinity purified polyclonal antibody (Catalog # PB9983) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMACR at approximately 42 kDa. The expected band size for AMACR is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9983-amacr-primary-antibodies-ihc-testing-2.jpgAnti-AMACR Antibody Picoband&reg; IHC analysis of AMACR using anti-AMACR antibody (PB9983). <br> AMACR was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AMACR Antibody (PB9983) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9983-amacr-primary-antibodies-ihc-testing-3.jpgAnti-AMACR Antibody Picoband&reg; IHC analysis of AMACR using anti-AMACR antibody (PB9983). <br> AMACR was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AMACR Antibody (PB9983) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9983-amacr-primary-antibodies-ihc-testing-4.jpgAnti-AMACR Antibody Picoband&reg; IHC analysis of AMACR using anti-AMACR antibody (PB9983). <br> AMACR was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AMACR Antibody (PB9983) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9983-amacr-primary-antibodies-if-testing-5.jpgAnti-AMACR Antibody Picoband&reg; IF analysis of AMACR using anti-AMACR antibody (PB9983). <br> AMACR was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AMACR Antibody (PB9983) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9152-pb9983-primary-antibodies-if-testing-1_1.jpgAnti-AMACR Antibody Picoband&reg;IF analysis of AMACR and TP63 using anti-AMACR antibody (PB9983) and anti-TP63 antibody (PB9152). <br> AMACR and TP63 were detected in paraffin-embedded sections of human prostatic hyperplasia tissue. Heat-mediated antigen retrieval was performed in EDTA buffer (pH 8.0). Tissue sections were blocked with 5% BSA. The sections were incubated overnight with 5 μg/mL rabbit Anti-TP63 antibody (PB9152). HRP-labeled secondary antibody was added dropwise and incubated for 30 minutes. TYR-570plus fluorescent dye reaction solution was then added dropwise and incubated at room temperature in the dark. Next, the sections were immersed in antigen retrieval buffer again to elute the bound antibodies. The staining steps were then repeated for the second round of labeling. Anti-AMACR antibody (PB9983) was diluted 1:50 and incubated with the sections overnight at 4°C. HRP-labeled secondary antibody was added dropwise and incubated for 30 minutes. After washing, TYR-520 plus fluorescent dye reaction solution was added dropwise and incubated at room temperature in the dark. Finally, DAPI staining solution was added dropwise to stain the nuclei, and the slides were sealed with an anti-fade mounting medium. The results were observed under a fluorescence microscope. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9983-amacr-primary-antibodies-fcm-testing-6.jpgAnti-AMACR Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-AMACR antibody (PB9983). <br> Overlay histogram showing HepG2 cells stained with PB9983 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMACR Antibody (PB9983, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-amhr2-picoband-trade-antibody-pb9984-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9984-amhr2-primary-antibodies-wb-testing-1.jpgAnti-AMHR2 Antibody Picoband&reg; Western blot analysis of AMHR2 using anti-AMHR2 antibody (PB9984). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human HL-60 whole cell lysates, <br> Lane 4: human Caco-2 whole cell lysates, <br> Lane 5: human K562 whole cell lysates, <br> Lane 6: human HepG2 whole cell lysates, <br> Lane 7: human PC-3 whole cell lysates, <br> Lane 8: human A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMHR2 antigen affinity purified polyclonal antibody (Catalog # PB9984) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMHR2 at approximately 65 kDa. The expected band size for AMHR2 is at 65 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9984-amhr2-primary-antibodies-ihc-testing-2.jpgAnti-AMHR2 Antibody Picoband&reg; IHC analysis of AMHR2 using anti-AMHR2 antibody (PB9984). <br> AMHR2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AMHR2 Antibody (PB9984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9984-amhr2-primary-antibodies-ihc-testing-3.jpgAnti-AMHR2 Antibody Picoband&reg; IHC analysis of AMHR2 using anti-AMHR2 antibody (PB9984). <br> AMHR2 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AMHR2 Antibody (PB9984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9984-amhr2-primary-antibodies-ihc-testing-4.jpgAnti-AMHR2 Antibody Picoband&reg; IHC analysis of AMHR2 using anti-AMHR2 antibody (PB9984). <br> AMHR2 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AMHR2 Antibody (PB9984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9984-amhr2-primary-antibodies-ihc-testing-5.jpgAnti-AMHR2 Antibody Picoband&reg; IHC analysis of AMHR2 using anti-AMHR2 antibody (PB9984). <br> AMHR2 was detected in paraffin-embedded section of human testis cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AMHR2 Antibody (PB9984) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9984-amhr2-primary-antibodies-if-testing-6.jpgAnti-AMHR2 Antibody Picoband&reg; IF analysis of AMHR2 using anti-AMHR2 antibody (PB9984). <br> AMHR2 was detected in an immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AMHR2 Antibody (PB9984) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9984-amhr2-primary-antibodies-fcm-testing-7.jpgAnti-AMHR2 Antibody Picoband&reg; Flow Cytometry analysis of K562 cells using anti-AMHR2 antibody (PB9984). <br>Overlay histogram showing K562 cells stained with PB9984 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMHR2 Antibody (PB9984, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apobec3g-picoband-trade-antibody-pb9985-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9985-apobec3g-primary-antibodies-wb-testing-1_1.jpgAnti-APOBEC3G Antibody Picoband&reg; Western blot analysis of APOBEC3G using anti-APOBEC3G antibody (PB9985). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: human HEL whole cell lysates,<br> Lane 3: human RT4 whole cell lysates,<br> Lane 4: rat testis tissue lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: mouse testis tissue lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3G antigen affinity purified polyclonal antibody (Catalog # PB9985) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3G at approximately 46 kDa. The expected band size for APOBEC3G is at 46 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9985-apobec3g-primary-antibodies-if-testing-2.jpgAnti-APOBEC3G Antibody Picoband&reg; IF analysis of APOBEC3G using anti-APOBEC3G antibody (PB9985). <br> APOBEC3G was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-APOBEC3G Antibody (PB9985) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apolipoprotein-e-picoband-trade-antibody-pb9986-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9986-1-WB-anti-apoe-apolipoprotein-e-picoband-antibody.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; Western blot analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9986). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates,<br> Lane 2: mouse kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein E antigen affinity purified polyclonal antibody (Catalog # PB9986) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein E at approximately 36 kDa. The expected band size for Apolipoprotein E is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9986-2-IHC-anti-apoe-apolipoprotein-e-picoband-antibody.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9986). <br> Apolipoprotein E was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Apolipoprotein E Antibody (PB9986) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9986-3-IHC-anti-apoe-apolipoprotein-e-picoband-antibody.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; IHC analysis of Apolipoprotein E using anti-Apolipoprotein E antibody (PB9986). <br> Apolipoprotein E was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Apolipoprotein E Antibody (PB9986) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9986-apoe-primary-antibodies-if-testing-4_1.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (PB9986). <br> Apolipoprotein E/APOE was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9986-apoe-primary-antibodies-if-testing-5.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (PB9986). <br> Apolipoprotein E/APOE was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9986-apoe-primary-antibodies-if-testing-6.jpgAnti-Apolipoprotein E/APOE Antibody Picoband&reg; IF analysis of Apolipoprotein E/APOE using anti-Apolipoprotein E/APOE antibody (PB9986). <br> Apolipoprotein E/APOE was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Apolipoprotein E/APOE Antibody (PB9986) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arf6-picoband-trade-antibody-pb9987-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9987-1-WB-anti-arf6-picoband-antibody.jpgAnti-ARF6 Antibody Picoband&reg; Western blot analysis of ARF6 using anti-ARF6 antibody (PB9987). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates&#44; <br> Lane 2: mouse kidney tissue lysates&#44; <br> Lane 3: MCF-7 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARF6 antigen affinity purified polyclonal antibody (Catalog # PB9987) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARF6 at approximately 20KD. The expected band size for ARF6 is at 20D.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9987-2.pngAnti-ARF6 Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-ARF6 antibody (PB9987). <br> Overlay histogram showing SiHa cells stained with PB9987 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARF6 Antibody (PB9987&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9987-3.jpgAnti-ARF6 Antibody Picoband&reg; IHC analysis of ARF6 using anti-ARF6 antibody (PB9987). <br> ARF6 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARF6 Antibody (PB9987) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9987-4.jpgAnti-ARF6 Antibody Picoband&reg; IHC analysis of ARF6 using anti-ARF6 antibody (PB9987). <br> ARF6 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ARF6 Antibody (PB9987) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9987-5.jpgAnti-ARF6 Antibody Picoband&reg; IF analysis of ARF6 using anti-ARF6 antibody (PB9987). <br> ARF6 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ARF6 Antibody (PB9987) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bcr-picoband-trade-antibody-pb9988-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9988-bcr-primary-antibodies-wb-testing-1.jpgAnti-Bcr Antibody Picoband&reg; Western blot analysis of Bcr using anti-Bcr antibody (PB9988). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates, <br> Lane 2: human 293T whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcr antigen affinity purified polyclonal antibody (Catalog # PB9988) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcr at approximately 143 kDa. The expected band size for Bcr is at 143 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9988-bcr-primary-antibodies-if-testing-2.jpgAnti-Bcr Antibody Picoband&reg; IF analysis of Bcr using anti-Bcr antibody (PB9988). <br> Bcr was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Bcr Antibody (PB9988) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9988-bcr-primary-antibodies-fcm-testing-3.pngAnti-Bcr Antibody Picoband&reg; Flow Cytometry analysis of HL-60 cells using anti-Bcr antibody (PB9988). <br>Overlay histogram showing HL-60 cells stained with PB9988 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bcr Antibody (PB9988, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ca2-picoband-trade-antibody-pb9989-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9989-ca2-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase II/CA2 Antibody Picoband&reg; Western blot analysis of CA2 using anti-CA2 antibody (PB9989). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human THP-1 whole cell lysates, <br> Lane 3: human HEL whole cell lysates, <br> Lane 4: rat kidney tissue lysates, <br> Lane 5: rat stomach tissue lysates, <br> Lane 6: mouse kidney tissue lysates, <br> Lane 7: mouse stomach tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA2 antigen affinity purified polyclonal antibody (Catalog # PB9989) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CA2 at approximately 29 kDa. The expected band size for CA2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9989-2-IHC-anti-ca2-carbonic-anhydrase-ii-picoband-antibody.jpgAnti-Carbonic Anhydrase II/CA2 Antibody Picoband&reg; IHC analysis of CA2 using anti-CA2 antibody (PB9989).<br> CA2 was detected in paraffin-embedded section of human gastric cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CA2 Antibody (PB9989) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9989-3-IHC-anti-ca2-carbonic-anhydrase-ii-picoband-antibody.jpgAnti-Carbonic Anhydrase II/CA2 Antibody Picoband&reg; IHC analysis of CA2 using anti-CA2 antibody (PB9989).<br> CA2 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CA2 Antibody (PB9989) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9989-ca2-primary-antibodies-fc-testing-3.jpgAnti-Carbonic Anhydrase II/CA2 Antibody Picoband&reg; Flow Cytometry analysis of HT-29 cells using anti-CA2 antibody (PB9989). <br>Overlay histogram showing HT-29 cells stained with PB9989 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CA2 Antibody (PB9989&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9989-ca2-primary-antibodies-fc-testing-4.jpgAnti-Carbonic Anhydrase II/CA2 Antibody Picoband&reg; Flow Cytometry analysis of SW480 cells using anti-CA2 antibody (PB9989). <br>Overlay histogram showing SW480 cells stained with PB9989 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CA2 Antibody (PB9989&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight&reg;488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-t1-picoband-trade-antibody-pb9990-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9990-1-WB-anti-cyclin-t1-picoband-antibody.jpgAnti-Cyclin T1/CCNT1 Antibody Picoband&reg; Western blot analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates,<br> Lane 2: mouse spleen tissue lysates,<br> Lane 3: JURKAT whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin T1 antigen affinity purified polyclonal antibody (Catalog # PB9990) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin T1 at approximately 81 kDa. The expected band size for Cyclin T1 is at 81 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9990-2-IHC-anti-cyclin-t1-picoband-antibody.jpgAnti-Cyclin T1/CCNT1 Antibody Picoband&reg; IHC analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990). <br> Cyclin T1 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cyclin T1 Antibody (PB9990) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9990-3-IHC-anti-cyclin-t1-picoband-antibody.jpgAnti-Cyclin T1/CCNT1 Antibody Picoband&reg; IHC analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990). <br> Cyclin T1 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cyclin T1 Antibody (PB9990) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9990-4-IHC-anti-cyclin-t1-picoband-antibody.jpgAnti-Cyclin T1/CCNT1 Antibody Picoband&reg; IHC analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990). <br> Cyclin T1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cyclin T1 Antibody (PB9990) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9990-5.pngAnti-Cyclin T1/CCNT1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Cyclin T1 antibody (PB9990). <br> Overlay histogram showing U20S cells stained with PB9990 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin T1 Antibody (PB9990&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9990-6.pngAnti-Cyclin T1/CCNT1 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-Cyclin T1 antibody (PB9990). <br> Overlay histogram showing U937 cells stained with PB9990 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Cyclin T1 Antibody (PB9990&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9990-7.jpgAnti-Cyclin T1/CCNT1 Antibody Picoband&reg; IF analysis of Cyclin T1 using anti-Cyclin T1 antibody (PB9990).<br> Cyclin T1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Cyclin T1 Antibody (PB9990) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cct2-picoband-trade-antibody-pb9992-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9992-cct2-primary-antibodies-wb-testing-1.jpgAnti-TCP1 beta/CCT2 Antibody Picoband&reg; Western blot analysis of CCT2 using anti-CCT2 antibody (PB9992). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human CACO-2 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: human MCF-7 whole cell lysates,<br> Lane 6: human HEL whole cell lysates,<br> Lane 7: human Raji whole cell lysates,<br> Lane 8: human SW620 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (Catalog # PB9992) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT2 at approximately 57 kDa. The expected band size for CCT2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9992-cct2-primary-antibodies-wb-testing-2.jpgAnti-TCP1 beta/CCT2 Antibody Picoband&reg; Western blot analysis of CCT2 using anti-CCT2 antibody (PB9992). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: rat spleen tissue lysates,<br> Lane 3: rat C6 whole cell lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: mouse brain tissue lysates,<br> Lane 6: mouse spleen tissue lysates,<br> Lane 7: mouse NIH/3T3 whole cell lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT2 antigen affinity purified polyclonal antibody (Catalog # PB9992) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT2 at approximately 57 kDa. The expected band size for CCT2 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9992-3-IHC-anti-tcp1-beta-picoband-antibody.jpgAnti-TCP1 beta/CCT2 Antibody Picoband&reg; IHC analysis of CCT2 using anti-CCT2 antibody (PB9992). <br> CCT2 was detected in paraffin-embedded section of rat gaster tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT2 Antibody (PB9992) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9992-4-IHC-anti-tcp1-beta-picoband-antibody.jpgAnti-TCP1 beta/CCT2 Antibody Picoband&reg; IHC analysis of CCT2 using anti-CCT2 antibody (PB9992). <br> CCT2 was detected in paraffin-embedded section of human testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT2 Antibody (PB9992) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9992-5-ihc-anti-tcp1-beta-picoband-antibody.jpgAnti-TCP1 beta/CCT2 Antibody Picoband&reg; IHC analysis of CCT2 using anti-CCT2 antibody (PB9992). <br> CCT2 was detected in paraffin-embedded section of mouse gaster tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CCT2 Antibody (PB9992) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9992-6.jpgAnti-TCP1 beta/CCT2 Antibody Picoband&reg; IF analysis of CCT2 using anti-CCT2 antibody (PB9992).<br> CCT2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT2 Antibody (PB9992) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9992-8.pngAnti-TCP1 beta/CCT2 Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-CCT2 antibody (PB9992). <br> Overlay histogram showing U251 cells stained with PB9992 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT2 Antibody (PB9992&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9992-7.jpgAnti-TCP1 beta/CCT2 Antibody Picoband&reg; IF analysis of CCT2 using anti-CCT2 antibody (PB9992).<br> CCT2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CCT2 Antibody (PB9992) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cct8-picoband-trade-antibody-pb9993-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9993-cct8-primary-antibodies-wb-testing-1.jpgAnti-TCP1 theta/CCT8 Antibody Picoband&reg; Western blot analysis of CCT8 using anti-CCT8 antibody (PB9993). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A431 whole cell lysates,<br> Lane 2: human Colo320 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates,<br> Lane 5: rat spleen tissue lysates,<br> Lane 6: rat PC-12 whole cell lysates,<br> Lane 7: mouse spleen tissue lysates,<br> Lane 8: mouse RAW264.7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT8 antigen affinity purified polyclonal antibody (Catalog # PB9993) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCT8 at approximately 60 kDa. The expected band size for CCT8 is at 60 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9993-2-IHC-anti-tcp1-theta-picoband-antibody.jpgAnti-TCP1 theta/CCT8 Antibody Picoband&reg; IHC analysis of CCT8 using anti-CCT8 antibody (PB9993). <br> CCT8 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CCT8 Antibody (PB9993) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9993-3-IHC-anti-tcp1-theta-picoband-antibody.jpgAnti-TCP1 theta/CCT8 Antibody Picoband&reg; IHC analysis of CCT8 using anti-CCT8 antibody (PB9993). <br> CCT8 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CCT8 Antibody (PB9993) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9993-4-IHC-anti-tcp1-theta-picoband-antibody.jpgAnti-TCP1 theta/CCT8 Antibody Picoband&reg; IHC analysis of CCT8 using anti-CCT8 antibody (PB9993). <br> CCT8 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CCT8 Antibody (PB9993) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9993-cct8-primary-antibodies-fcm-testing-5.jpgAnti-TCP1 theta/CCT8 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-CCT8 antibody (PB9993). <br> Overlay histogram showing HepG2 cells stained with PB9993 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT8 Antibody (PB9993, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9993-cct8-primary-antibodies-if-testing-6.jpgAnti-TCP1 theta/CCT8 Antibody Picoband&reg; IF analysis of CCT8 using anti-CCT8 antibody (PB9993). <br> CCT8 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CCT8 Antibody (PB9993) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pd-l1-picoband-trade-antibody-pb9994-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9994-1-WB-anti-pd-l1-b7-h1-picoband-antibody.jpgAnti-PD-L1/CD274 Antibody Picoband&reg; Western blot analysis of PD-L1 using anti-PD-L1 antibody (PB9994). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1 antigen affinity purified polyclonal antibody (Catalog # PB9994) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1 at approximately 39 kDa. The expected band size for PD-L1 is at 33 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk6-picoband-trade-antibody-pb9995-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9995-cdk6-primary-antibodies-ihc-testing-1.jpgAnti-Cdk6 Antibody Picoband&reg; IHC analysis of Cdk6 using anti-Cdk6 antibody (PB9995).<br> Cdk6 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (PB9995) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9995-fonc-11-648985-g001.jpgAnti-Cdk6 Antibody Picoband&reg;PD promotes the anti-tumor effects of sorafenib in PC3 cells. (A) . The effects of PD, sorafenib and PD plus sorafenib on cell viability. (B, C) . The changes in the cell membrane and nucleus after treatment with PD alone, sorafenib (Sor) alone or PD plus sorafenib. (D) . The induction of apoptosis by PD, Sor and PD + Sor. (E) . The protein expression levels of Caspase 3, C-Caspase 3, PARP and C-PARP were examined after cells were treated with 10 μM PD alone, 10 μM sorafenib alone or PD plus sorafenib for 6 h. (F) . The ability of cells to achieve colony growth was assessed after treatment with PD alone, sorafenib alone or PD plus sorafenib for 10 days. (G) . The proliferation of cells was monitored using the CFDA SE assay after treatment with PD alone, sorafenib alone or PD plus sorafenib for 5 days. (H, I) . The cell cycle distribution of PC3 cells following treatment with PD alone, sorafenib alone or PD plus sorafenib for 24 h after pre-treatment with (H) or without (I) 2 mM thymidine. (J) . Changes in the protein expression levels of CDK4, CDK6 and cyclin D1 after treatment with 5 μM PD alone, 2.5 μM sorafenib alone or PD plus sorafenib for 24 h. * p < 0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/articles/10.3389/fonc.2021.648985/full'>34026624</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9995-cdk6-primary-antibodies-ihc-testing-2.jpgAnti-Cdk6 Antibody Picoband&reg; IHC analysis of Cdk6 using anti-Cdk6 antibody (PB9995).<br> Cdk6 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (PB9995) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9995-cdk6-primary-antibodies-ihc-testing-3.jpgAnti-Cdk6 Antibody Picoband&reg; IHC analysis of Cdk6 using anti-Cdk6 antibody (PB9995).<br> Cdk6 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Cdk6 Antibody (PB9995) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ciita-picoband-trade-antibody-pb9996-boster.html2026-03-25T05:22:38+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9996-1-WB-anti-ciita-picoband-antibody.jpgAnti-CIITA Antibody Picoband&reg; Western blot analysis of CIITA using anti-CIITA antibody (PB9996). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates,<br> Lane 2: mouse thymus tissue lysates,<br> Lane 3: MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIITA antigen affinity purified polyclonal antibody (Catalog # PB9996) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIITA at approximately 123 kDa. The expected band size for CIITA is at 124 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9996-2-IHC-anti-ciita-picoband-antibody.jpgAnti-CIITA Antibody Picoband&reg; IHC analysis of CIITA using anti-CIITA antibody (PB9996). <br> CIITA was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CIITA Antibody (PB9996) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9996-3-IHC-anti-ciita-picoband-antibody.jpgAnti-CIITA Antibody Picoband&reg; IHC analysis of CIITA using anti-CIITA antibody (PB9996). <br> CIITA was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CIITA Antibody (PB9996) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9996-4-IHC-anti-ciita-picoband-antibody.jpgAnti-CIITA Antibody Picoband&reg; IHC analysis of CIITA using anti-CIITA antibody (PB9996). <br> CIITA was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CIITA Antibody (PB9996) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cpb2-picoband-trade-antibody-pb9997-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9997-1-WB-anti-tafi-cpb2-picoband-antibody.jpgAnti-Carboxypeptidase B2/CPB2 Antibody Picoband&reg; Western blot analysis of CPB2 using anti-CPB2 antibody (PB9997). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates,<br> Lane 2: HEPG2 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPB2 antigen affinity purified polyclonal antibody (Catalog # PB9997) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPB2 at approximately 48 kDa, 60 kDa. The expected band size for CPB2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9997-2-IHC-anti-tafi-cpb2-picoband-antibody.jpgAnti-Carboxypeptidase B2/CPB2 Antibody Picoband&reg; IHC analysis of CPB2 using anti-CPB2 antibody (PB9997). <br> CPB2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CPB2 Antibody (PB9997) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9997-cpb2-primary-antibodies-elisa-testing-3.jpgAnti-Carboxypeptidase B2/CPB2 Antibody Picoband&reg; Sandwich ELISA - Recombinant human CPB2 protein standard curve.<br> Use in combination with reagents from Human CPB2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1502). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cpb2-picoband-trade-antibody-pb9998-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb9998-1-WB-anti-tafi-cpb2-picoband-antibody.jpgAnti-Carboxypeptidase B2/CPB2 Antibody Picoband&reg; Western blot analysis of CPB2 using anti-CPB2 antibody (PB9998). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPB2 antigen affinity purified polyclonal antibody (Catalog # PB9998) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPB2 at approximately 48 kDa. The expected band size for CPB2 is at 49 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bca1-picoband-trade-antibody-pb9999-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9999-bca1-primary-antibodies-wb-testing-1.jpgAnti-BCA1/CXCL13 Antibody Picoband&reg;Western blot analysis of BCA1/CXCL13 using anti-BCA1/CXCL13 antibody (PB9999). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. <br> Lane 1: recombinant mouse BCA1/CXCL13 protein 10ng. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCA1/CXCL13 antigen affinity purified polyclonal antibody (PB9999) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BCA1/CXCL13 at approximately 11 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9999-bca1-primary-antibodies-ihc-testing-1.jpgAnti-BCA1/CXCL13 Antibody Picoband&reg;IHC analysis of BCA1/CXCL13 using anti-BCA1/CXCL13 antibody (PB9999). <br> BCA1/CXCL13 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-BCA1/CXCL13 Antibody (PB9999) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddah1-picoband-trade-antibody-pb10000-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-ddah1-primary-antibodies-wb-testing-1.jpgAnti-DDAH1 Antibody Picoband&reg; Western blot analysis of DDAH1 using anti-DDAH1 antibody (PB10000). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HUVEC whole cell lysates,<br> Lane 2: human HepG2 whole cell lysates,<br> Lane 3: human Caco-2 whole cell lysates,<br> Lane 4: human SH-SY5Y whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified polyclonal antibody (Catalog # PB10000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH1 at approximately 37 kDa. The expected band size for DDAH1 is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-ddah1-primary-antibodies-ihc-testing-4.jpgAnti-DDAH1 Antibody Picoband&reg;IHC analysis of DDAH1 using anti-DDAH1 antibody (PB10000). <br>DDAH1 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DDAH1 Antibody (PB10000) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-fphar-16-1565713-g008.jpgAnti-DDAH1 Antibody Picoband&reg;The protein expression of Oatp4c1, Oct2, Ddah1and Mate1 in rat kidney. (A) Long-term administration of BUP and followed by single-dose administration of DIG; (B) Long-term administration of BUP and followed by single-dose administration of drug cocktail. Cocktail consisted of a single i.v. dose of MET at 5 mg/kg, a single i.v. dose of FUR at 4 mg/kg and a single p.o. dose of RSV at 25 mg/kg. Data are expressed as mean ± SD (n = 5). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Data were analyzed by one-way ANOVA.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2025.1565713/full'>40474975</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-2.jpgAnti-DDAH1 Antibody Picoband&reg; IHC analysis of DDAH1 using anti-DDAH1 antibody (PB10000).<br> DDAH1 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDAH1 Antibody (PB10000) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-3.jpgAnti-DDAH1 Antibody Picoband&reg; IHC analysis of DDAH1 using anti-DDAH1 antibody (PB10000).<br> DDAH1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-DDAH1 Antibody (PB10000) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-4.jpgAnti-DDAH1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-DDAH1 antibody (PB10000). <br> Overlay histogram showing A431 cells stained with PB10000 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDAH1 Antibody (PB10000&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-5.jpgAnti-DDAH1 Antibody Picoband&reg; IF analysis of DDAH1 using anti-DDAH1 antibody (PB10000). <br> DDAH1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDAH1 Antibody (PB10000) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10000-ddah1-primary-antibodies-wb-testing-6.jpgAnti-DDAH1 Antibody Picoband&reg; Western blot analysis of DDAH1 using anti-DDAH1 antibody (PB10000). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human Caco-2 whole cell lysates, <br> Lane 3: human SH-SY5Y whole cell lysates, <br> Lane 4: rat brain tissue lysates, <br> Lane 5: rat liver tissue lysates, <br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH1 antigen affinity purified polyclonal antibody (PB10000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for DDAH1 at approximately 36 kDa. The expected band size for DDAH1 is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ddah2-picoband-trade-antibody-pb10001-boster.html2026-03-24T05:05:48+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10001-1-WB-anti-ddah2-picoband-antibody.jpgAnti-DDAH2 Antibody Picoband&reg; Western blot analysis of DDAH2 using anti-DDAH2 antibody (PB10001). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: mouse lung tissue lysates, <br> Lane 3: human placenta tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDAH2 antigen affinity purified polyclonal antibody (Catalog # PB10001) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDAH2 at approximately 24 kDa. The expected band size for DDAH2 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10001-2-IHC-anti-ddah2-picoband-antibody.jpgAnti-DDAH2 Antibody Picoband&reg; IHC analysis of DDAH2 using anti-DDAH2 antibody (PB10001). <br> DDAH2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DDAH2 Antibody (PB10001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10001-3-IHC-anti-ddah2-picoband-antibody.jpgAnti-DDAH2 Antibody Picoband&reg; IHC analysis of DDAH2 using anti-DDAH2 antibody (PB10001). <br> DDAH2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DDAH2 Antibody (PB10001) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10001-4.jpgAnti-DDAH2 Antibody Picoband&reg; IF analysis of DDAH2 using anti-DDAH2 antibody (PB10001). <br> DDAH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-DDAH2 Antibody (PB10001) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10001-5.jpgAnti-DDAH2 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-DDAH2 antibody (PB10001).<br>Overlay histogram showing CACO-2 cells stained with PB10001 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDAH2 Antibody (PB10001,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hmgb2-picoband-trade-antibody-pb10002-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10002-hmgb2-primary-antibodies-wb-testing-1.jpgAnti-HMGB2 Antibody Picoband&reg; Western blot analysis of HMGB2 using anti-HMGB2 antibody (PB10002). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: rat PC-12 whole cell lysates,<br> Lane 3: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HMGB2 antigen affinity purified polyclonal antibody (Catalog # PB10002) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HMGB2 at approximately 24 kDa. The expected band size for HMGB2 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10002-2-IHC-anti-hmgb2-hmg2-picoband-antibody.jpgAnti-HMGB2 Antibody Picoband&reg; IHC analysis of HMGB2 using anti-HMGB2 antibody (PB10002). <br> HMGB2 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HMGB2 Antibody (PB10002) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10002-3-IHC-anti-hmgb2-hmg2-picoband-antibody.jpgAnti-HMGB2 Antibody Picoband&reg; IHC analysis of HMGB2 using anti-HMGB2 antibody (PB10002). <br> HMGB2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HMGB2 Antibody (PB10002) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10002-4-IHC-anti-hmgb2-hmg2-picoband-antibody.jpgAnti-HMGB2 Antibody Picoband&reg; IHC analysis of HMGB2 using anti-HMGB2 antibody (PB10002). <br> HMGB2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HMGB2 Antibody (PB10002) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10002-5.jpgAnti-HMGB2 Antibody Picoband&reg; IF analysis of HMGB2 using anti-HMGB2 antibody (PB10002).<br> HMGB2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-HMGB2 Antibody (PB10002) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10002-hmgb2-primary-antibodies-if-testing-6.jpgAnti-HMGB2 Antibody Picoband&reg; IF analysis of HMGB2 and Tubulin alpha using anti-HMGB2 antibody (PB10002) and anti-Tubulin alpha antibody (M03989-3).<br> HMGB2 and Tubulin alpha were detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 μg/mL rabbit anti-HMGB2 antibody (PB10002) and mouse anti-Tubulin alpha Antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10002-hmgb2-primary-antibodies-if-testing-7.jpgAnti-HMGB2 Antibody Picoband&reg; IF analysis of HMGB2 using anti-HMGB2 antibody (PB10002). <br> HMGB2 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-HMGB2 Antibody (PB10002) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10002-hmgb2-primary-antibodies-fcm-testing-8.jpgAnti-HMGB2 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-HMGB2 antibody (PB10002). <br> Overlay histogram showing A431 cells stained with PB10002 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HMGB2 Antibody (PB10002&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-alpha-internexin-picoband-trade-antibody-pb10003-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-wb-testing-1_1.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; Western blot analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: human Hela whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Internexin antigen affinity purified polyclonal antibody (Catalog # PB10003) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Internexin at approximately 62-66 kDa. The expected band size for Alpha Internexin is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-ihc-testing-2.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; IHC analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Alpha Internexin was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin Antibody (PB10003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-ihc-testing-6.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg;IHC analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (PB10003). <br>Alpha Internexin/INA was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin/INA Antibody (PB10003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-ihc-testing-3.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; IHC analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Alpha Internexin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin Antibody (PB10003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-ihc-testing-4.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; IHC analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Alpha Internexin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin Antibody (PB10003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-ihc-testing-5.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; IHC analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Alpha Internexin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Alpha Internexin Antibody (PB10003) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-if-testing-6.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; IF analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Alpha Internexin was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Alpha Internexin Antibody (PB10003) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-if-testing-7.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; IF analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Alpha Internexin was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Alpha Internexin Antibody (PB10003) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-if-testing-8.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; IF analysis of Alpha Internexin using anti-Alpha Internexin antibody (PB10003). <br> Alpha Internexin was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Alpha Internexin Antibody (PB10003) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10003-ina-primary-antibodies-fcm-testing-9.jpgAnti-Alpha Internexin/INA Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-Alpha Internexin antibody (PB10003). <br> Overlay histogram showing SiHa cells stained with PB10003 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha Internexin Antibody (PB10003, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-itln1-picoband-trade-antibody-pb10004-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10004-1-WB-anti-itln1-omentin-picoband-antibody.jpgAnti-ITLN1 Antibody Picoband&reg; Western blot analysis of ITLN1 using anti-ITLN1 antibody (PB10004). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SW620 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITLN1 antigen affinity purified polyclonal antibody (Catalog # PB10004) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITLN1 at approximately 43 kDa. The expected band size for ITLN1 is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10004-2-IHC-anti-itln1-omentin-picoband-antibody.jpgAnti-ITLN1 Antibody Picoband&reg; IHC analysis of ITLN1 using anti-ITLN1 antibody (PB10004). <br> ITLN1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ITLN1 Antibody (PB10004) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10004-itln1-primary-antibodies-if-testing-3.jpgAnti-ITLN1 Antibody Picoband&reg; IF analysis of ITLN1 using anti-ITLN1 antibody (PB10004). <br> ITLN1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ITLN1 Antibody (PB10004) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10004-itln1-primary-antibodies-fcm-testing-4.jpgAnti-ITLN1 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-ITLN1 antibody (PB10004).<br>Overlay histogram showing U937 cells stained with PB10004 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ITLN1 Antibody (PB10004,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kallikrein-8-picoband-trade-antibody-pb10005-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10005-1-WB-anti-klk8-kallikrein-8-picoband-antibody.jpgAnti-Kallikrein 8/KLK8 Antibody Picoband&reg; Western blot analysis of Kallikrein 8 using anti-Kallikrein 8 antibody (PB10005). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 8 antigen affinity purified polyclonal antibody (Catalog # PB10005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 8 at approximately 28 kDa. The expected band size for Kallikrein 8 is at 28 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tak1-picoband-trade-antibody-pb10006-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10006-tak1-primary-antibodies-wb-testing-1_1.jpgAnti-TAK1/MAP3K7 Antibody Picoband&reg;Western blot analysis of TAK1/MAP3K7 using anti-TAK1/MAP3K7 antibody (PB10006). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human A375 whole cell lysates, <br> Lane 4: human COLO320 whole cell lysates, <br> Lane 5: rat heart tissue lysates, <br> Lane 6: rat H9C2 whole cell lysates, <br> Lane 7: mouse heart tissue lysates, <br> Lane 8: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAK1/MAP3K7 antigen affinity purified polyclonal antibody (Catalog # PB10006) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TAK1/MAP3K7 at approximately 75 kDa. The expected band size for TAK1/MAP3K7 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10006-tak1-primary-antibodies-if-testing-1.jpgAnti-TAK1/MAP3K7 Antibody Picoband&reg;IF analysis of TAK1/MAP3K7 using anti-TAK1/MAP3K7 antibody (PB10006). <br> TAK1/MAP3K7 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TAK1/MAP3K7 Antibody (PB10006) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10006-tak1-primary-antibodies-fcm-testing-1.jpgAnti-TAK1/MAP3K7 Antibody Picoband&reg;Flow Cytometry analysis of A375 cells using anti-TAK1/MAP3K7 antibody (PB10006). <br> Overlay histogram showing A375 cells stained with PB10006 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-TAK1/MAP3K7 Antibody (PB10006, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-map3k8-picoband-trade-antibody-pb10007-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-wb-testing-1.jpgAnti-MAP3K8 Antibody Picoband&reg; Western blot analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human HEPG2 whole cell lysates, <br> Lane 2: human HELA whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human THP-1 whole cell lysates, <br> Lane 6: human Daudi whole cell lysates, <br> Lane 7: rat liver tissue lysates, <br> Lane 8: mouse liver tissue lysates. <br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K8 antigen affinity purified polyclonal antibody (Catalog # PB10007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAP3K8 at approximately 53KD. The expected band size for MAP3K8 is at 53KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-if-testing-2.jpgAnti-MAP3K8 Antibody Picoband&reg; IF analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). <br> MAP3K8 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-MAP3K8 Antibody (PB10007) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-fcm-testing-3.pngAnti-MAP3K8 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-MAP3K8 antibody (PB10007). <br>Overlay histogram showing U937 cells stained with PB10007 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MAP3K8 Antibody (PB10007, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-ihc-testing-4.jpgAnti-MAP3K8 Antibody Picoband&reg; IHC analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). <br> MAP3K8 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP3K8 Antibody (PB10007) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-ihc-testing-5.jpgAnti-MAP3K8 Antibody Picoband&reg; IHC analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). <br> MAP3K8 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP3K8 Antibody (PB10007) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-ihc-testing-6.jpgAnti-MAP3K8 Antibody Picoband&reg; IHC analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). <br> MAP3K8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP3K8 Antibody (PB10007) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-ihc-testing-7.jpgAnti-MAP3K8 Antibody Picoband&reg; IHC analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). <br> MAP3K8 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP3K8 Antibody (PB10007) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10007-map3k8-primary-antibody-ihc-testing-8.jpgAnti-MAP3K8 Antibody Picoband&reg; IHC analysis of MAP3K8 using anti-MAP3K8 antibody (PB10007). <br> MAP3K8 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MAP3K8 Antibody (PB10007) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mmp-9-picoband-trade-antibody-pb10008-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10008-1-WB-anti-mmp-9-picoband-antibody.jpgAnti-MMP9 Antibody Picoband&reg; Western blot analysis of MMP-9 using anti-MMP-9 antibody (PB10008). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: NRK whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP-9 antigen affinity purified polyclonal antibody (Catalog # PB10008) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MMP-9 at approximately 78 kDa, 92 kDa. The expected band size for MMP-9 is at 79 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10008-2-IHC-anti-mmp-9-picoband-antibody.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP-9 using anti-MMP-9 antibody (PB10008). <br> MMP-9 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MMP-9 Antibody (PB10008) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10008-3-IHC-anti-mmp-9-picoband-antibody.jpgAnti-MMP9 Antibody Picoband&reg; IHC analysis of MMP-9 using anti-MMP-9 antibody (PB10008). <br> MMP-9 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MMP-9 Antibody (PB10008) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-visfatin-picoband-trade-antibody-pb10009-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10009-visfatin-primary-antibodies-wb-testing-1.jpgAnti-Visfatin/NAMPT Antibody Picoband&reg; Western blot analysis of Visfatin using anti-Visfatin antibody (PB10009). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human U87 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human Daudi whole cell lysates,<br> Lane 4: human U20S whole cell lysates,<br> Lane 5: human K562 whole cell lysates,<br> Lane 6: human A549 whole cell lysates,<br> Lane 7: human HL-60 whole cell lysates,<br> Lane 8: mouse testis tissue lysates,<br> Lane 9: mouse RAW264.7 whole cell lysates,<br> Lane 10: rat brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Visfatin antigen affinity purified polyclonal antibody (Catalog # PB10009) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Visfatin at approximately 54 kDa. The expected band size for Visfatin is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10009-2-IHC-anti-visfatin-picoband-antibody.jpgAnti-Visfatin/NAMPT Antibody Picoband&reg; IHC analysis of Visfatin using anti-Visfatin antibody (PB10009). <br> Visfatin was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Visfatin Antibody (PB10009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10009-3-IHC-anti-visfatin-picoband-antibody.jpgAnti-Visfatin/NAMPT Antibody Picoband&reg; IHC analysis of Visfatin using anti-Visfatin antibody (PB10009). <br> Visfatin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Visfatin Antibody (PB10009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10009-4-IHC-anti-visfatin-picoband-antibody.jpgAnti-Visfatin/NAMPT Antibody Picoband&reg; IHC analysis of Visfatin using anti-Visfatin antibody (PB10009). <br> Visfatin was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Visfatin Antibody (PB10009) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10009-nampt-primary-antibodies-fcm-testing-5.pngAnti-Visfatin/NAMPT Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-Visfatin antibody (PB10009). <br> Overlay histogram showing U20S cells stained with PB10009 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Visfatin Antibody (PB10009, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10009-nampt-primary-antibodies-if-testing-6.jpgAnti-Visfatin/NAMPT Antibody Picoband&reg; IF analysis of Visfatin/NAMPT using anti-Visfatin/NAMPT antibody (PB10009). <br> Visfatin/NAMPT was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Visfatin/NAMPT Antibody (PB10009) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-b7-dc-picoband-trade-antibody-pb10010-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10010-1-WB-anti-b7-dc-picoband-antibody.jpgAnti-B7 DC/PDCD1LG2 Antibody Picoband&reg; Western blot analysis of B7 DC using anti-B7 DC antibody (PB10010). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7 DC antigen affinity purified polyclonal antibody (Catalog # PB10010) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7 DC at approximately 37 kDa. The expected band size for B7 DC is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pf4-picoband-trade-antibody-pb10011-boster.html2026-04-01T05:01:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10011-pf4-primary-antibodies-wb-testing-1.jpgAnti-PF4 Antibody Picoband&reg;Western blot analysis of PF4 using anti-PF4 antibody (PB10011). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates,<br> Lane 2: rat lung tissue lysates,<br> Lane 3: mouse spleen tissue lysates,<br> Lane 4: mouse lung tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (PB10011) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11 kDa. The expected band size for PF4 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10011-pf4-primary-antibodies-ihc-testing-1.jpgAnti-PF4 Antibody Picoband&reg;IHC analysis of PF4 using anti-PF4 antibody (PB10011). <br> PF4 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PF4 Antibody (PB10011) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pf4-picoband-trade-antibody-pb10012-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10012-gr4_lrg.jpgAnti-PF4 Antibody Picoband&reg;C5ar1 knockdown in CD45+ brown adipocytes promote PF4 release to inhibit brown adipocyte maturation. (A) Relative mRNA expression of the cytokines genes (Pf4, Ccl3, Ccl4, Ccl12) of C5ar1 knockdown differentiated CD45+ adipocytes compared to the control group (n = 6). (B) The concentration of PF4 in the supernatant of C5ar1 knockdown differentiated CD45+ adipocytes compared to the control group (n = 3). (C) Pf4 mRNA expression in differentiated CD45− and CD45+ adipocytes (n = 6). (D) Concentration of PF4 in the supernatant of differentiated CD45− and CD45+ adipocytes (n = 3). (E) Pf4 mRNA expression of BAT from Control and C5ar1 AKO neonatal mice (n = 8). (F) Concentration of PF4 in BAT of Control and C5ar1 AKO neonatal mice (n = 6). (G) Relative mRNA expression of the indicated genes of adipocyte differentiation of CD45− ASCs cultured without or with 20 ng/mL PF4 (n = 6). (H) Immunoblotting for UCP1 and PPARγ of adipocyte differentiation of CD45− ASCs cultured without or with 20 ng/mL PF4 (n = 3). (I) Relative mRNA expression of the indicated genes from adipocyte differentiation of CD45− ASCs cultured in conditioned media from shNC, shC5ar1, shPf4 or shC5ar1+shPf4 CD45+ adipocytes (n = 3). (J) Immunoblotting for UCP1 and PPARγ of adipocyte differentiation of CD45− ASCs cultured in conditioned media from shNC, shC5ar1, shPf4 or shC5ar1+shPf4 CD45+ adipocytes (n = 3). (K) Graphical abstract of this study: The loss of C5ar1 in CD45+ adipocytes increased Pf4 mRNA level and increased the secretion of PF4. PF4 inhibited the maturity and thermogenesis ability of both CD45+ and CD45− adipocytes. Statistical significance was assessed by two-tailed Student’s t test (A–G) or one-way ANOVA (I). Data are represented as mean ± SEM ∗ ≤0.05, ∗∗ ≤0.01, ∗∗∗ ≤0.005. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.cell.com/iscience/fulltext/S2589-0042(24)02486-6'>39758991</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10012-pf4-primary-antibodies-wb-testing-1.jpgAnti-PF4 Antibody Picoband&reg; Western blot analysis of PF4 using anti-PF4 antibody (PB10012). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # PB10012) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11 kDa. The expected band size for PF4 is at 11 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10012-pf4-primary-antibodies-ihc-testing-2.jpgAnti-PF4 Antibody Picoband&reg; IHC analysis of PF4 using anti-PF4 antibody (PB10012). <br> PF4 was detected in a paraffin-embedded section of mouse lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PF4 Antibody (PB10012) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-perilipin-3-picoband-trade-antibody-pb10013-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10013-plin3-primary-antibodies-wb-testing-1.jpgAnti-Perilipin 3/PLIN3 Antibody Picoband&reg;Western blot analysis of Perilipin 3/PLIN3 using anti-Perilipin 3/PLIN3 antibody (PB10013). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates,<br> Lane 2: human THP-1 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human U2OS whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Perilipin 3/PLIN3 antigen affinity purified polyclonal antibody (PB10013) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Perilipin 3/PLIN3 at approximately 47 kDa. The expected band size for Perilipin 3/PLIN3 is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10013-plin3-primary-antibodies-ihc-testing-1.jpgAnti-Perilipin 3/PLIN3 Antibody Picoband&reg;IHC analysis of Perilipin 3/PLIN3 using anti-Perilipin 3/PLIN3 antibody (PB10013). <br>Perilipin 3/PLIN3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Perilipin 3/PLIN3 Antibody (PB10013) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10013-plin3-primary-antibodies-ihc-testing-2.jpgAnti-Perilipin 3/PLIN3 Antibody Picoband&reg;IHC analysis of Perilipin 3/PLIN3 using anti-Perilipin 3/PLIN3 antibody (PB10013). <br>Perilipin 3/PLIN3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Perilipin 3/PLIN3 Antibody (PB10013) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-peptide-yy-picoband-trade-antibody-pb10014-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10014-1_1.jpgAnti-Peptide YY/PYY Antibody IHC analysis of Peptide YY using anti-Peptide YY antibody (PB10014).<br> Peptide YY was detected in paraffin-embedded section of rat pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10014-2.jpgAnti-Peptide YY/PYY Antibody IHC analysis of Peptide YY using anti-Peptide YY antibody (PB10014).<br> Peptide YY was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10014-3.jpgAnti-Peptide YY/PYY Antibody IHC analysis of Peptide YY using anti-Peptide YY antibody (PB10014).<br> Peptide YY was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10014-4.jpgAnti-Peptide YY/PYY Antibody IHC analysis of Peptide YY using anti-Peptide YY antibody (PB10014).<br> Peptide YY was detected in paraffin-embedded section of mouse pancreas tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10014-5.jpgAnti-Peptide YY/PYY Antibody IHC analysis of Peptide YY using anti-Peptide YY antibody (PB10014).<br> Peptide YY was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10014-pyy-primary-antibodies-if-testing-6.jpgAnti-Peptide YY/PYY Antibody IF analysis of Peptide YY using anti-Peptide YY antibody (PB10014). <br> Peptide YY was detected in a paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Peptide YY Antibody (PB10014) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rankl-picoband-trade-antibody-pb10015-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10015-1-WB-anti-tnfsf11-rankl-picoband-antibody.jpgAnti-RANKL/TNFSF11 Antibody Picoband&reg; Western blot analysis of RANKL using anti-RANKL antibody (PB10015). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANKL antigen affinity purified polyclonal antibody (Catalog # PB10015) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANKL at approximately 40 kDa. The expected band size for RANKL is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10015-medscimonit-26-e922372-g007.jpgAnti-RANKL/TNFSF11 Antibody Picoband&reg;( A ) Photomicrography of the osteoclast differentiation factor (ODF) immunostaining at the mesial periodontium of the first maxillary molar (400×). p – periodontal space; r – root. Bar=20 μm. ( B ) Graphical representation of the ODF expression on the compression side of all studied groups. Data are expressed as mean±standard deviations (SD); * P <0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7193222/&orig_host=www.ncbi.nlm.nih.gov'>32323648</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10015-medscimonit-26-e922372-g009.jpgAnti-RANKL/TNFSF11 Antibody Picoband&reg;( A ) mRNA expression levels of RANKL (receptor activator of nuclear factor kappa-B ligand) at different time points in the compression side. ( B ) Protein expression levels of RANKL in the compression side on day 3. ( C ) Protein expression levels of RANKL in the compression side on day 7. ( D ) Protein expression levels of RANKL in the compression side on day 14. Data are expressed as mean±standard deviation (SD); * P <0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7193222/&orig_host=www.ncbi.nlm.nih.gov'>32323648</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mast-cell-tryptase-picoband-trade-antibody-pb10016-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10016-1-WB-anti-mast-cell-tryptase-picoband-antibody.jpgAnti-Mast Cell Tryptase/TPSAB1 Antibody Picoband&reg; Western blot analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody (PB10016). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: 293T whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mast Cell Tryptase antigen affinity purified polyclonal antibody (Catalog # PB10016) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mast Cell Tryptase at approximately 30 kDa. The expected band size for Mast Cell Tryptase is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10016-2-IHC-anti-mast-cell-tryptase-picoband-antibody.jpgAnti-Mast Cell Tryptase/TPSAB1 Antibody Picoband&reg; IHC analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody (PB10016). <br> Mast Cell Tryptase was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Mast Cell Tryptase Antibody (PB10016) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10016-tpsab1-primary-antibodies-ihc-testing-3.jpgAnti-Mast Cell Tryptase/TPSAB1 Antibody Picoband&reg; IHC analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody (PB10016).<br> Mast Cell Tryptase was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Mast Cell Tryptase Antibody (PB10016) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10016-tpsab1-primary-antibodies-ihc-testing-4.jpgAnti-Mast Cell Tryptase/TPSAB1 Antibody Picoband&reg; IHC analysis of Mast Cell Tryptase using anti-Mast Cell Tryptase antibody (PB10016).<br> Mast Cell Tryptase was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Mast Cell Tryptase Antibody (PB10016) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10016-tryptase-primary-antibodies-elisa-testing-5.jpgAnti-Mast Cell Tryptase/TPSAB1 Antibody Picoband&reg; Sandwich ELISA - Recombinant human Tryptase/TPSAB1,B2 protein standard curve.<br> Use in combination with reagents from Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0898). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sybl1-picoband-trade-antibody-pb10017-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10017-1-WB-anti-sybl1-picoband-antibody.jpgAnti-SYBL1/VAMP7 Antibody Picoband&reg; Western blot analysis of SYBL1 using anti-SYBL1 antibody (PB10017). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYBL1 antigen affinity purified polyclonal antibody (Catalog # PB10017) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYBL1 at approximately 25 kDa. The expected band size for SYBL1 is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-zwint-picoband-trade-antibody-pb10018-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10018-1-WB-anti-zwint-picoband-antibody.jpgAnti-ZWINT Antibody Picoband&reg; Western blot analysis of ZWINT using anti-ZWINT antibody (PB10018). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat skeletal muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # PB10018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 31 kDa. The expected band size for ZWINT is at 31 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10018-2-IHC-anti-zwint-picoband-antibody.jpgAnti-ZWINT Antibody Picoband&reg; IHC analysis of ZWINT using anti-ZWINT antibody (PB10018). <br> ZWINT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZWINT Antibody (PB10018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10018-zwint-primary-antibodies-ihc-testing-4.jpgAnti-ZWINT Antibody Picoband&reg;IHC analysis of ZWINT using anti-ZWINT antibody (PB10018). <br>ZWINT was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ZWINT Antibody (PB10018) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10018-3-IHC-anti-zwint-picoband-antibody.jpgAnti-ZWINT Antibody Picoband&reg; IHC analysis of ZWINT using anti-ZWINT antibody (PB10018). <br> ZWINT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZWINT Antibody (PB10018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/picokine-elisa-kits/mouse-nov-ccn3-picokine-trade-elisa-kit-ek0834-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0834_1.pngMouse NOV/CCN3 ELISA Kit PicoKine®Mouse NOV/CCN3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-meteorin-metrn-picokine-trade-elisa-kit-ek1551-boster.html2026-03-24T05:05:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1551_1.pngMouse Meteorin/METRN ELISA Kit PicoKine®Mouse Meteorin/METRN PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-meteorin-like-metrnl-picokine-trade-elisa-kit-ek1550-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1550_2.pngMouse Meteorin-like/METRNL ELISA Kit PicoKine®Mouse Meteorin-like/METRNL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-epcr-picokine-trade-elisa-kit-ek1439-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1439_1.pngMouse EPCR ELISA Kit PicoKine®Mouse EPCR PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-hemojuvelin-rgm-c-picokine-trade-elisa-kit-ek1369-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1369_1.pngHuman Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®Human Hemojuvelin/RGM-C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-hemojuvelin-rgm-c-picokine-trade-elisa-kit-ek1371-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1371_1.pngRat Hemojuvelin/RGM-C/HJV ELISA Kit PicoKine®Rat Hemojuvelin/RGM-C PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl27-ctack-picokine-trade-elisa-kit-ek0689-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0689_1.pngMouse CCL27/CTACK ELISA Kit PicoKine®Mouse CCL27/CTACK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fgf1-picokine-trade-elisa-kit-ek0339-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0339_1.pngHuman FGF1/Fgf Acidic ELISA Kit PicoKine®Human FGF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-dll4-picokine-trade-elisa-kit-ek1570-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1570_1.pngRat DLL4 ELISA Kit PicoKine®Rat DLL4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-beta-ig-h3-tgfbi-picokine-trade-elisa-kit-ek1571-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1571_1.pngRat beta IG-H3/TGFBI ELISA Kit PicoKine®Rat beta IG-H3/TGFBI PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-alcam-picokine-trade-elisa-kit-ek1572-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1572.jpgRat ALCAM/CD166 ELISA Kit PicoKine®Rat ALCAM PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-endostatin-picokine-trade-elisa-kit-ek1377-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1377_1.pngRat Endostatin ELISA Kit PicoKine®Rat Endostatin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-tnfsf11-rankl-picokine-trade-elisa-kit-ek1559-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1559_1.pngRat TNFSF11/RANKL ELISA Kit PicoKine®Rat TNFSF11/RANKL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-sclerostin-sost-picokine-trade-elisa-kit-ek1565-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1565_1.pngRat Sclerostin/SOST ELISA Kit PicoKine®Rat Sclerostin/SOST PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-wif1-picokine-trade-elisa-kit-ek1564-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1564.jpgRat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®Rat WIF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-ncam1-cd56-picokine-trade-elisa-kit-ek1562-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1562.jpgRat CD56/NCAM-1 ELISA Kit PicoKine®Rat NCAM1/CD56 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-gdf-15-picokine-trade-elisa-kit-ek1561-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1561_1.pngRat GDF-15 ELISA Kit PicoKine®Rat GDF-15 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-fabp4-picokine-trade-elisa-kit-ek1573-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1573_1.pngRat FABP4/A Fabp ELISA Kit PicoKine®Rat FABP4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rat-angiopoietin-2-picokine-trade-elisa-kit-ek1574-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1574_1.pngRat Angiopoietin-2 ELISA Kit PicoKine®Rat Angiopoietin-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-lif-picokine-trade-elisa-kit-ek0580-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0580.jpgMouse LIF ELISA Kit PicoKine®Mouse LIF PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abat-picoband-trade-antibody-pb10019-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10019-abat-primary-antibodies-wb-testing-1.jpgAnti-ABAT Antibody Picoband&reg; Western blot analysis of ABAT using anti-ABAT antibody (PB10019). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: rat brain tissue lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: mouse brain tissue lysates, <br> Lane 5: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABAT antigen affinity purified polyclonal antibody (Catalog # PB10019) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABAT at approximately 54 kDa. The expected band size for ABAT is at 54 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10019-abat-primary-antibodies-if-testing-2.jpgAnti-ABAT Antibody Picoband&reg; IF analysis of ABAT using anti-ABAT antibody (PB10019). <br> ABAT was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABAT Antibody (PB10019) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10019-abat-primary-antibodies-fcm-testing-3.jpgAnti-ABAT Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-ABAT antibody (PB10019). <br>Overlay histogram showing CACO-2 cells stained with PB10019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABAT Antibody (PB10019, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abce1-picoband-trade-antibody-pb10020-boster.html2026-03-24T05:05:50+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10020-abce1-primary-antibodies-wb-testing-1.jpgAnti-ABCE1 Antibody Picoband&reg; Western blot analysis of ABCE1 using anti-ABCE1 antibody (PB10020). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human U2OS whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: mouse brian tissue lysates,<br> Lane 7: mouse 4T1 whole cell lysates,<br> Lane 8: mouse NIH/3T3 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (Catalog # PB10020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10020-abce1-primary-antibodies-if-testing-2.jpgAnti-ABCE1 Antibody Picoband&reg; IF analysis of ABCE1 using anti-ABCE1 antibody (PB10020). <br> ABCE1 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABCE1 Antibody (PB10020) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10020-abce1-primary-antibodies-ip-testing-3.jpgAnti-ABCE1 Antibody Picoband&reg; Immunoprecipitating ABCE1 in A431 whole cell lysate. <br>Western blot analysis of ABCE1 using anti-ABCE1 antibody (PB10020). <br>Lane 1: A431 whole cell lysates (30ug), <br>Lane 2: Rabbit control IgG instead of anti-ABCE1 antibody in A431 whole cell lysate, <br>Lane 3: anti-ABCE1 antibody (2μg) + A431 whole cell lysate (500μg). <br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (PB10020) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Heavy Chain). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10020-abce1-primary-antibodies-fcm-testing-4.jpgAnti-ABCE1 Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-ABCE1 antibody (PB10020). <br> Overlay histogram showing U251 cells stained with PB10020 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCE1 Antibody (PB10020, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abhd5-picoband-trade-antibody-pb10021-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10021-1-WB-anti-abhd5-picoband-antibody.jpgAnti-Abhd5 Antibody Picoband&reg; Western blot analysis of Abhd5 using anti-Abhd5 antibody (PB10021). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates&#44; <br> Lane 2: A431 whole cell lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Abhd5 antigen affinity purified polyclonal antibody (Catalog # PB10021) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Abhd5 at approximately 43KD. The expected band size for Abhd5 is at 39KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10021-2-IHC-anti-abhd5-picoband-antibody.jpgAnti-Abhd5 Antibody Picoband&reg; IHC analysis of Abhd5 using anti-Abhd5 antibody (PB10021). <br> Abhd5 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Abhd5 Antibody (PB10021) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10021-3.jpgAnti-Abhd5 Antibody Picoband&reg; IF analysis of Abhd5 using anti-Abhd5 antibody (PB10021).<br> Abhd5 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Abhd5 Antibody ((PB10021) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acaa2-picoband-trade-antibody-pb10022-boster.html2026-04-05T05:00:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10022-acaa2-primary-antibody-wb-testing-1.jpgAnti-ACAA2 Antibody Picoband&reg; Western blot analysis of ACAA2 using anti-ACAA2 antibody (PB10022). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat lung tissue lysates, <br> Lane 3: rat brain tissue lysates, <br> Lane 4: rat kidney tissue lysates,<br> Lane 5: human Hela whole cell lysates, <br> Lane 6: mouse HEPA1-6 whole cell lysates, <br> Lane 7: mouse NIH/3T3 whole cell lysates, <br> Lane 8: mouse SP2/0 whole cell lysates, <br> Lane 9: mouse lung tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACAA2 antigen affinity purified polyclonal antibody (Catalog # PB10022) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACAA2 at approximately 42 kDa. The expected band size for ACAA2 is at 42 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10022-2-IHC-anti-acaa2-picoband-antibody.jpgAnti-ACAA2 Antibody Picoband&reg; IHC analysis of ACAA2 using anti-ACAA2 antibody (PB10022). <br> ACAA2 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACAA2 Antibody (PB10022) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10022-3.jpgAnti-ACAA2 Antibody Picoband&reg; IF analysis of ACAA2 using anti-ACAA2 antibody (PB10022).<br> ACAA2 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ACAA2 Antibody ((PB10022) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10022-4.pngAnti-ACAA2 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-ACAA2 antibody (PB10022). <br> Overlay histogram showing HepG2 cells stained with PB10022 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACAA2 Antibody (PB10022&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acadvl-picoband-trade-antibody-pb10023-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10023-acadvl-primary-antibodies-wb-testing-1.jpgAnti-ACADVL Antibody Picoband&reg; Western blot analysis of ACADVL using anti-ACADVL antibody (PB10023). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SiHa whole cell lysates,<br> Lane 3: human A431 whole cell lysates,<br> Lane 4: human U251 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat heart tissue lysates,<br> Lane 7: mouse liver tissue lysates,<br> Lane 8: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACADVL antigen affinity purified polyclonal antibody (Catalog # PB10023) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACADVL at approximately 70 kDa. The expected band size for ACADVL is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10023-acadvl-primary-antibodies-ihc-testing-2.jpgAnti-ACADVL Antibody Picoband&reg; IHC analysis of ACADVL using anti-ACADVL antibody (PB10023). <br> ACADVL was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACADVL Antibody (PB10023) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10023-acadvl-primary-antibodies-ihc-testing-3.jpgAnti-ACADVL Antibody Picoband&reg; IHC analysis of ACADVL using anti-ACADVL antibody (PB10023). <br> ACADVL was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACADVL Antibody (PB10023) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10023-acadvl-primary-antibodies-ihc-testing-4.jpgAnti-ACADVL Antibody Picoband&reg; IHC analysis of ACADVL using anti-ACADVL antibody (PB10023). <br> ACADVL was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACADVL Antibody (PB10023) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10023-acadvl-primary-antibodies-if-testing-5.jpgAnti-ACADVL Antibody Picoband&reg; IF analysis of ACADVL using anti-ACADVL antibody (PB10023). <br> ACADVL was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ACADVL Antibody (PB10023) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10023-acadvl-primary-antibodies-ip-testing-6.jpgAnti-ACADVL Antibody Picoband&reg; Immunoprecipitating ACADVL in Hela whole cell lysate. <br>Western blot analysis of ACADVL using anti-ACADVL antibody (PB10023). <br>Lane 1: Hela whole cell lysates (30ug), <br>Lane 2: Rabbit control IgG instead of anti-ACADVL antibody in Hela whole cell lysate, <br>Lane 3: anti-ACADVL antibody (2μg) + Hela whole cell lysate (500μg). <br>After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACADVL antigen affinity purified polyclonal antibody (PB10023) at a dilution of 0.5 μg/mL and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for ACADVL at approximately 70 kDa. The expected band size for ACADVL is at 70 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10023-acadvl-primary-antibodies-fcm-testing-7.jpgAnti-ACADVL Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-ACADVL antibody (PB10023). <br> Overlay histogram showing U251 cells stained with PB10023 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACADVL Antibody (PB10023, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atp-citrate-lyase-picoband-trade-antibody-pb10024-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-wb-testing-1.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; Western blot analysis of ACLY using anti-ACLY antibody (PB10024). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human A549 whole cell lysates, <br> Lane 2: human MOLT4 whole cell lysates, <br> Lane 3: human U87 whole cell lysates, <br> Lane 4: human U251 whole cell lysates, <br> Lane 5: human 293T whole cell lysates, <br> Lane 6: human Hela whole cell lysates, <br> Lane 7: human T47D whole cell lysates, <br> Lane 8: human HepG2 whole cell lysates, <br> Lane 9: rat pancreas tissue lysates, <br> Lane 10: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACLY antigen affinity purified polyclonal antibody (Catalog # PB10024) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACLY at approximately 125 kDa. The expected band size for ACLY is at 125 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-ihc-testing-2.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IHC analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-ihc-testing-3.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IHC analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-ihc-testing-4.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IHC analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-ihc-testing-5.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IHC analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-ihc-testing-6.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IHC analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-ihc-testing-7.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IHC analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-ihc-testing-8.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IHC analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-if-testing-9.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; IF analysis of ACLY using anti-ACLY antibody (PB10024). <br> ACLY was detected in an immunocytochemical section of A594 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ACLY Antibody (PB10024) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10024-acly-primary-antibody-fcm-testing-10.jpgAnti-ATP citrate lyase/ACLY Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-ACLY antibody (PB10024). <br>Overlay histogram showing HepG2 cells stained with PB10024 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACLY Antibody (PB10024, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acsl1-picoband-trade-antibody-pb10025-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10025-acsl1-primary-antibodies-wb-testing-1.jpgAnti-ACSL1 Antibody Picoband&reg; Western blot analysis of ACSL1 using anti-ACSL1 antibody (PB10025). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human U87 whole cell lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: rat heart tissue lysates, <br> Lane 5: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACSL1 antigen affinity purified polyclonal antibody (Catalog # PB10025) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACSL1 at approximately 78 kDa. The expected band size for ACSL1 is at 78 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10025-2-IHC-anti-acsl1-picoband-antibody.jpgAnti-ACSL1 Antibody Picoband&reg; IHC analysis of ACSL1 using anti-ACSL1 antibody (PB10025). <br> ACSL1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ACSL1 Antibody (PB10025) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10025-3-IHC-anti-acsl1-picoband-antibody.jpgAnti-ACSL1 Antibody Picoband&reg; IHC analysis of ACSL1 using anti-ACSL1 antibody (PB10025). <br> ACSL1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ACSL1 Antibody (PB10025) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10025-4-IHC-anti-acsl1-picoband-antibody.jpgAnti-ACSL1 Antibody Picoband&reg; IHC analysis of ACSL1 using anti-ACSL1 antibody (PB10025). <br> ACSL1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ACSL1 Antibody (PB10025) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10025-acsl1-primary-antibodies-if-testing-5.jpgAnti-ACSL1 Antibody Picoband&reg; IF analysis of ACSL1 using anti-ACSL1antibody (PB10025). <br> ACSL1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-ACSL1 Antibody (PB10025) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10025-acsl1-primary-antibodies-fcm-testing-6.pngAnti-ACSL1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ACSL1 antibody (PB10025). <br>Overlay histogram showing A431 cells stained with PB10025 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACSL1 Antibody (PB10025, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-actn3-picoband-trade-antibody-pb10026-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10026-actn3-primary-antibodies-wb-testing-1.jpgAnti-ACTN3 Antibody Picoband&reg; Western blot analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody (PB10026). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HT1080 whole cell lysates, <br> Lane 2: rat skeletal muscle tissue lysates, <br> Lane 3: rat skeletal muscle tissue lysates, <br> Lane 4: mouse skeletal muscle tissue lysates, <br> Lane 5: mouse skeletal muscle tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACTN3/Alpha Actinin 3 antigen affinity purified polyclonal antibody (Catalog # PB10026) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACTN3/Alpha Actinin 3 at approximately 103 kDa. The expected band size for ACTN3/Alpha Actinin 3 is at 103 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10026-13395_2021_279_fig2_html.pngAnti-ACTN3 Antibody Picoband&reg;Major patterns of protein distribution according to fiber type in human skeletal muscle. The fiber type distribution of representative proteins (indicated by gene name) is shown for each pattern, with values expressed as per cent of the maximal value. ACTN3 , α-actinin-3; CYCS , cytochrome c; MYL2 , MLC-2 slow; MYLK2 , myosin light chain kinase 2; RYR1 , ryanodine receptor 1; TNNC2 , fast troponin C <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13395-021-00279-0'>34727990</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10026-13395_2021_279_fig3_html.pngAnti-ACTN3 Antibody Picoband&reg;Myofibrillar proteins: fiber type distribution of representative proteins. Proteins showing similar distribution in the different types are not shown in the figure. Values are expressed as per cent of the maximal value. ACTN2 , α-actinin-2; ACTN3 , α-actinin-3; CAPZA1 , CapZ α1; LMOD2 , leiomodin-2; LRRC39 , Myomasp; MYL1 , MLC1/3-fast; MYL2 , MLC-2slow; MYL3 , MLC-1 slow; MYLPF , MLC2-fast; MYL6B , MLC-1sa; MYBPC1 , myosin-binding protein C1; MYBPC2 , myosin-binding protein C2; MYBPH , myosin-binding protein H; MYOM2 , myomesin 2; MYOM3 , myomesin 3; MYOZ2 , myozenin 2; MYOZ3 , myozenin 3; TNNC1 , slow troponin C; TNNC2 , fast troponin C; TNNI1 , slow troponin I; TNNI2 , fast troponin I; TNNT1 , slow troponin T; TNNT2 , fast troponin T; TPM1 , α-tropomyosin; TPM3 , γ-tropomyosin <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13395-021-00279-0'>34727990</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10026-13395_2021_279_fig8_html.pngAnti-ACTN3 Antibody Picoband&reg;Fiber type–specific distribution of selected proteins revealed by immunofluorescence analysis. Fiber types are labeled as 1 (type 1), 2A (type 2A), or 2X (type 2X). Hybrid 2A–2X fibers are labeled by asterisks. A – D Type 1–specific proteins. Each panel shows on the left a section stained for PGM5/aciculin ( A ), PDLIM1 ( B ), MCU ( C ), or IDH2 ( D ) and on the right a serial section stained with MYH-specific antibodies to reveal the three fiber types. No pure type 2X fibers is present in A and B . E – F Type 2–specific proteins. Each panel shows on the left a section stained for ACTN3 ( E ) or XIRP2 ( F ), on the center a serial section stained for MYH-specific antibodies to reveal the three fiber types, and on the right a section stained with anti-MYH antibody BF-35, which reacts with MYH7 and MYH2 but not with MYH1, thus stains all fiber types, except pure type 2X fibers. Note that whereas ACTN3 is especially abundant in type 2X fibers, as well as in hybrid 2A–2X fibers, XIRP2 is present at higher levels in both 2A and 2X fibers. WGA counterstain was applied to all sections, except those processed with the three anti-MYHs antibodies <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13395-021-00279-0'>34727990</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10026-2-IHC-anti-actn3-alpha-actinin-3-picoband-antibody.jpgAnti-ACTN3 Antibody Picoband&reg; IHC analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody (PB10026).<br> ACTN3/Alpha Actinin 3 was detected in paraffin-embedded section of mouse skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTN3/Alpha Actinin 3 Antibody (PB10026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10026-3-IHC-anti-actn3-alpha-actinin-3-picoband-antibody.jpgAnti-ACTN3 Antibody Picoband&reg; IHC analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody (PB10026).<br> ACTN3/Alpha Actinin 3 was detected in paraffin-embedded section of rat skeletal muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTN3/Alpha Actinin 3 Antibody (PB10026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10026-4-IHC-anti-actn3-alpha-actinin-3-picoband-antibody.jpgAnti-ACTN3 Antibody Picoband&reg; IHC analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody (PB10026).<br> ACTN3/Alpha Actinin 3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTN3/Alpha Actinin 3 Antibody (PB10026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10026-actn3-primary-antibodies-if-testing-1.jpgAnti-ACTN3 Antibody Picoband&reg;IF analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody (PB10026). <br> ACTN3/Alpha Actinin 3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-ACTN3/Alpha Actinin 3 Antibody (PB10026) overnight at 4°C. FITC Conjugated Goat Anti-Rabbit IgG (BA1105) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10026-actn3-primary-antibodies-fc-testing-5.jpgAnti-ACTN3 Antibody Picoband&reg; Flow Cytometry analysis of WISH cells using anti-ACTN3 antibody (PB10026). <br>Overlay histogram showing WISH cells stained with PB10026 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ACTN3 Antibody (PB10026&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-acvr2a-picoband-trade-antibody-pb10027-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10027-1-WB-anti-acvr2a-actr-ii-picoband-antibody.jpgAnti-Activin Receptor Type IIA/ACVR2A Antibody Picoband&reg; Western blot analysis of ACVR2A using anti-ACVR2A antibody (PB10027). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: HELA whole cell lysates, <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACVR2A antigen affinity purified polyclonal antibody (Catalog # PB10027) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACVR2A at approximately 75 kDa. The expected band size for ACVR2A is at 58 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10027-2-IHC-anti-acvr2a-actr-ii-picoband-antibody.jpgAnti-Activin Receptor Type IIA/ACVR2A Antibody Picoband&reg; IHC analysis of ACVR2A using anti-ACVR2A antibody (PB10027). <br> ACVR2A was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ACVR2A Antibody (PB10027) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10027-3.jpgAnti-Activin Receptor Type IIA/ACVR2A Antibody Picoband&reg; IF analysis of ACVR2A using anti-ACVR2A antibody (PB10027). <br> ACVR2A was detected in immunocytochemical section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ACVR2A Antibody (PB10027) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10027-4.jpgAnti-Activin Receptor Type IIA/ACVR2A Antibody Picoband&reg; Flow Cytometry analysis of HEPA1-6 cells using anti-ACVR2A antibody (PB10027).<br>Overlay histogram showing HEPA1-6 cells stained with PB10027 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ACVR2A Antibody (PB10027,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10027-5.jpgAnti-Activin Receptor Type IIA/ACVR2A Antibody Picoband&reg; Flow Cytometry analysis of RH35 cells using anti-ACVR2A antibody (PB10027).<br>Overlay histogram showing RH35 cells stained with PB10027 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ACVR2A Antibody (PB10027,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adamts13-picoband-trade-antibody-pb10028-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10028-adamts13-primary-antibodies-wb-testing-1.jpgAnti-ADAMTS13 Antibody Picoband&reg; Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody (PB10028). <br>Western blot analysis of ADAMTS13 expression in human HepG2 whole cell lysates 50ug&#44; ADAMTS13 at 154KD under non-reducing conditions and 200 kD under reducing conditions were detected using rabbit anti-ADAMTS13 Antigen Affinity purified polyclonal antibody (Catalog # PB10028) at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10028-adamts13-primary-antibodies-wb-testing-2.jpgAnti-ADAMTS13 Antibody Picoband&reg; Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody (PB10028). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse liver tissue lysates&#44; <br> Lane 2: rat brain tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS13 antigen affinity purified polyclonal antibody (Catalog # PB10028) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADAMTS13 at approximately 154KD. The expected band size for ADAMTS13 is at 154KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10028-adamts13-primary-antibodies-elisa-testing-3.jpgAnti-ADAMTS13 Antibody Picoband&reg; Sandwich ELISA - Recombinant human ADAMTS13 protein standard curve.<br> Use in combination with reagents from Human ADAMTS13 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0927). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adh1a-picoband-trade-antibody-pb10029-boster.html2026-04-01T05:01:25+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-13020_2023_753_fig6_html.pngAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg;The metabolic enzymes ADH1, ALDH2 and CYP2E1 are significantly up-regulated by alcohol, and further up-regulated by BGXJW administration. A – E The mRNA levels of ADH1, ALDH2, ALDH1, CYP2E1 in liver tissues were subjected to RT-qPCR analysis. E Levels of ADH1, ALDH2, CYP2E1 in liver lysates after indicated treatment were determined by western blot, F Liver tissue sections were subjected to immunohistochemistry analysis of CYP2E1. All data are expressed as the mean ± SEM (n = 3). nsP > 0.05, *P < 0.05 vs ctrl group; nsP > 0.05 vs EtOH group. Crtl contrl, EtOH ethanol model, BGXJW Bao-Gan-Xing-Jiu-Wan <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s13020-023-00753-5'>37127639</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-fnut-09-848918-g010.jpgAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg; Effects of WEATs on ADH1A/ADH1B/ADH1G expression in the liver of mice. Representative immunoblots (A) and IHC images (B) of ADH1A/ADH1B/ADH1G expression in the liver. Quantification of ADH1A/ADH1B/ADH1G expression by western blotting (C) and IHC (D) . Each value represents the mean ± SEM ( n = 10).<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9169692/&orig_host=www.ncbi.nlm.nih.gov'>35677547</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-adh1a-primary-antibody-wb-testing-1.jpgAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg; Western blot analysis of ADH1A using anti-ADH1A antibody (PB10029). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HCCT tissue lysates, <br> Lane 2: human HCCP tissue lysates, <br> Lane 3: rat liver tissue lysates.<br> Lane 4: rat kidney tissue lysates.<br> Lane 5: mouse liver tissue lysates.<br> Lane 6: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH1A antigen affinity purified polyclonal antibody (Catalog # PB10029) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH1A at approximately 40 kDa. The expected band size for ADH1A is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-adh1a-primary-antibody-ihc-testing-2.jpgAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg; IHC analysis of ADH1A using anti-ADH1A antibody (PB10029). <br> ADH1A was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-adh1a-primary-antibody-fcm-testing-6.jpgAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-ADH1A antibody (PB10029). <br>Overlay histogram showing U20S cells stained with PB10029 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADH1A Antibody (PB10029, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-adh1a-primary-antibody-ihc-testing-3.jpgAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg; IHC analysis of ADH1A using anti-ADH1A antibody (PB10029). <br> ADH1A was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-adh1a-primary-antibody-ihc-testing-4.jpgAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg; IHC analysis of ADH1A using anti-ADH1A antibody (PB10029). <br> ADH1A was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10029-adh1a-primary-antibody-if-testing-5.jpgAnti-Alcohol Dehydrogenase/ADH1A Antibody Picoband&reg; IF analysis of ADH1A using anti-ADH1A antibody (PB10029). <br> ADH1A was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ADH1A Antibody (PB10029) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adh4-picoband-trade-antibody-pb10030-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10030-adh4-primary-antibodies-wb-testing-1.jpgAnti-ADH4 Antibody Picoband&reg;Western blot analysis of PADH4 using anti-ADH4 antibody (PB10030). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: huamn HepG2 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: rat liver tissue lysates, <br> Lane 4: rat RH35 whole cell lysates, <br> Lane 5: mouse iver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH4 antigen affinity purified polyclonal antibody (PB10030) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADH4 at approximately 40 kDa. The expected band size for ADH4 is at 40 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adk-picoband-trade-antibody-pb10031-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-wb-testing-1.jpgAnti-ADK Antibody Picoband&reg; Western blot analysis of ADK using anti-ADK antibody (PB10031). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates, <br> Lane 2: human PC-3 whole cell lysates, <br> Lane 3: human T47D whole cell lysates, <br> Lane 4: human U937 whole cell lysates, <br> Lane 5: human U251 whole cell lysates, <br> Lane 6: human HepG2 whole cell lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADK antigen affinity purified polyclonal antibody (Catalog # PB10031) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADK at approximately 45 kDa. The expected band size for ADK is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-wb-testing-2.jpgAnti-ADK Antibody Picoband&reg; Western blot analysis of ADK using anti-ADK antibody (PB10031). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat kidney tissue lysates, <br> Lane 3: rat stomach tissue lysates, <br> Lane 4: rat PC-12 whole cell lysates, <br> Lane 5: mouse kidney tissue lysates, <br> Lane 6: mouse stomach tissue lysates, <br> Lane 7: mouse NIH/3T3 whole cell lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADK antigen affinity purified polyclonal antibody (Catalog # PB10031) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADK at approximately 45 kDa. The expected band size for ADK is at 45 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-6.jpgAnti-ADK Antibody Picoband&reg;IHC analysis of ADK using anti-ADK antibody (PB10031). <br>ADK was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADK Antibody (PB10031) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-7.jpgAnti-ADK Antibody Picoband&reg;IHC analysis of ADK using anti-ADK antibody (PB10031). <br>ADK was detected in a paraffin-embedded section of human liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADK Antibody (PB10031) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-3.jpgAnti-ADK Antibody Picoband&reg; IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADK Antibody (PB10031) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-4.jpgAnti-ADK Antibody Picoband&reg; IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADK Antibody (PB10031) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-5.jpgAnti-ADK Antibody Picoband&reg; IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADK Antibody (PB10031) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-if-testing-6.jpgAnti-ADK Antibody Picoband&reg; IF analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ADK Antibody (PB10031) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-4.pngAnti-ADK Antibody Picoband&reg;IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ADK Antibody (PB10031) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-5.pngAnti-ADK Antibody Picoband&reg;IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ADK Antibody (PB10031) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-6.pngAnti-ADK Antibody Picoband&reg;IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of human tosil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ADK Antibody (PB10031) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-7.pngAnti-ADK Antibody Picoband&reg;IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ADK Antibody (PB10031) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-ihc-testing-8.pngAnti-ADK Antibody Picoband&reg;IHC analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ADK Antibody (PB10031) for 30 minutes at 37°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-if-testing-2.pngAnti-ADK Antibody Picoband&reg;IF analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX1 Antibody (PB10031) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10031-adk-primary-antibodies-if-testing-3.pngAnti-ADK Antibody Picoband&reg;IF analysis of ADK using anti-ADK antibody (PB10031). <br> ADK was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DDX1 Antibody (PB10031) overnight at 4°C. DyLight 594 Conjugated AffiniPure Goat Anti-rabbit IgG(H+L) (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ado-picoband-trade-antibody-pb10032-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-wb-testing-1_1.jpgAnti-ADO Antibody Picoband&reg; Western blot analysis of ADO using anti-ADO antibody (PB10032). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human PC-3 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: rat brain tissue lysates,<br> Lane 4: mouse heart tissue lysates,<br> Lane 5: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADO antigen affinity purified polyclonal antibody (Catalog # PB10032) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADO at approximately 28, 30 kDa. The expected band size for ADO is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-fc-testing-2.jpgAnti-ADO Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-ADO antibody (PB10032). <br>Overlay histogram showing A549 cells stained with PB10032 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADO Antibody (PB10032&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-fc-testing-3.jpgAnti-ADO Antibody Picoband&reg; Flow Cytometry analysis of U251 cells using anti-ADO antibody (PB10032). <br>Overlay histogram showing U251 cells stained with PB10032 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADO Antibody (PB10032&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-fc-testing-4.jpgAnti-ADO Antibody Picoband&reg;4. Flow Cytometry analysis of Hela cells using anti-ADO antibody (PB10032).<br>Overlay histogram showing Hela cells stained with PB10032 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADO Antibody (PB10032&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-fc-testing-5.jpgAnti-ADO Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-ADO antibody (PB10032). <br>Overlay histogram showing PC-3 cells stained with PB10032 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADO Antibody (PB10032&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-if-testing-6.jpgAnti-ADO Antibody Picoband&reg; IF analysis of ADO using anti-ADO antibody (PB10032). <br> ADO was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ADO Antibody (PB10032) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-ihc-testing-7.jpgAnti-ADO Antibody Picoband&reg; IHC analysis of ADO using anti-ADO antibody (PB10032). <br> ADO was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ADO Antibody (PB10032) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10032-ado-primary-antibodies-ihc-testing-8.jpgAnti-ADO Antibody Picoband&reg; IHC analysis of ADO using anti-ADO antibody (PB10032). <br> ADO was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-ADO Antibody (PB10032) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adenylate-kinase-1-picoband-trade-antibody-pb10033-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10033-ak1-primary-antibodies-wb-testing-1.jpgAnti-Adenylate Kinase 1/AK1 Antibody Picoband&reg; Western blot analysis of AK1 using anti-AK1 antibody (PB10033). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human SiHa whole cell lysates,<br> Lane 3: rat heart tissue lysates,<br> Lane 4: mouse lung tissue lysates,<br> Lane 5: mouse heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AK1 antigen affinity purified polyclonal antibody (Catalog # PB10033) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AK1 at approximately 22 kDa. The expected band size for AK1 is at 22 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10033-ak1-primary-antibodies-ihc-testing-2.jpgAnti-Adenylate Kinase 1/AK1 Antibody Picoband&reg; IHC analysis of AK1 using anti-AK1 antibody (PB10033). <br> AK1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AK1 Antibody (PB10033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10033-ak1-primary-antibodies-ihc-testing-3.jpgAnti-Adenylate Kinase 1/AK1 Antibody Picoband&reg; IHC analysis of AK1 using anti-AK1 antibody (PB10033). <br> AK1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AK1 Antibody (PB10033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10033-ak1-primary-antibodies-ihc-testing-4.jpgAnti-Adenylate Kinase 1/AK1 Antibody Picoband&reg; IHC analysis of AK1 using anti-AK1 antibody (PB10033). <br> AK1 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AK1 Antibody (PB10033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10033-ak1-primary-antibodies-ihc-testing-5.jpgAnti-Adenylate Kinase 1/AK1 Antibody Picoband&reg; IHC analysis of AK1 using anti-AK1 antibody (PB10033). <br> AK1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AK1 Antibody (PB10033) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10033-ak1-primary-antibodies-fcm-testing-6.jpgAnti-Adenylate Kinase 1/AK1 Antibody Picoband&reg; Flow Cytometry analysis of PC-3 cells using anti-AK1 antibody (PB10033). <br> Overlay histogram showing PC-3 cells stained with PB10033 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AK1 Antibody (PB10033, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ak2-picoband-trade-antibody-pb10034-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10034-ak2-primary-antibodies-wb-testing-1.jpgAnti-AK2 Antibody Picoband&reg; Western blot analysis of AK2 using anti-AK2 antibody (PB10034). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HL-60 whole cell lysates, <br> Lane 3: human 293T whole cell lysates, <br> Lane 4: human HepG2 whole cell lysates, <br> Lane 5: rat heart tissue lysates, <br> Lane 6: rat liver tissue lysates, <br> Lane 7: mouse heart tissue lysates, <br> Lane 8: mouse liver tissue lysates, <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AK2 antigen affinity purified polyclonal antibody (Catalog # PB10034) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AK2 at approximately 26 kDa. The expected band size for AK2 is at 26 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10034-2-IHC-anti-ak2-picoband-antibody.jpgAnti-AK2 Antibody Picoband&reg; IHC analysis of AK2 using anti-AK2 antibody (PB10034). <br> AK2 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AK2 Antibody (PB10034) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10034-3-IHC-anti-ak2-picoband-antibody.jpgAnti-AK2 Antibody Picoband&reg; IHC analysis of AK2 using anti-AK2 antibody (PB10034). <br> AK2 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AK2 Antibody (PB10034) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10034-4-IHC-anti-ak2-picoband-antibody.jpgAnti-AK2 Antibody Picoband&reg; IHC analysis of AK2 using anti-AK2 antibody (PB10034). <br> AK2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AK2 Antibody (PB10034) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10034-5.jpgAnti-AK2 Antibody Picoband&reg; IF analysis of AK2 using anti-AK2 antibody (PB10034). <br> AK2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-AK2 Antibody (PB10034) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akr1b1-picoband-trade-antibody-pb10035-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10035-akr1b1-primary-antibodies-wb-testing-1.jpgAnti-AKR1B1 Antibody Picoband&reg; Western blot analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SiHa whole cell lysates, <br> Lane 2: rat testis tissue lysates, <br> Lane 3: mouse NIH/3T3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1B1 antigen affinity purified polyclonal antibody (Catalog # PB10035) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1B1 at approximately 36 kDa. The expected band size for AKR1B1 is at 36 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10035-fonc-13-1089090-g005.jpgAnti-AKR1B1 Antibody Picoband&reg;Cell differentiation trajectories in CRC obtained by pseudotime analysis. (A–D) tSNE map shows the results of the dimensionality reduction and clustering analysis of S112 (A) , S115 (B) , S114 (C) , and 927 (D) (up). Results of pseudotime cell trajectory in S112 (A) , S115 (B) , S114 (C) , and S927 (D) (down). (E) Twelve genes were screened by invasive modules. (F) The relationship of STC1 expression level and cancer stage/progression-free survival in colon cancer (up) and rectal cancer (down) from TCGA database. (G) The relationship of CES1 expression level and cancer stage in colon cancer (up) and rectal cancer (down) from TCGA database. (H) Immunohistochemical staining showed the expression of AKR1B1(left panel), STC1(middle panel), and CD47(right panel) in Mucosa and cancer(up) and Invasive margin(down) (n=45). The scale bars on the lower right are 100 µm. *P < 0.05, **P < 0.01 and ***P < 0.001. NS, not significant difference.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9928961/&orig_host=www.ncbi.nlm.nih.gov'>36816947</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10035-fonc-13-1089090-g006.jpgAnti-AKR1B1 Antibody Picoband&reg;Knockdown of AKR1B1 inhibited cell proliferation, migration, and invasion. (A, B) The transcriptional level (A) and protein level (B) of AKR1B1 were detected in multiple colorectal cancer cell lines (SW480, SW620, HCT116, LoVo, and HT29) using RT-PCR and WB. (C, D) The transcriptional level (C) and protein level (D) of AKR1B1 were detected in the control and knockdown groups of SW620 using RT-PCR and WB. (E, F) The transcriptional level (E) and protein level (F) of AKR1B1 were detected in the control and knockdown groups of HCT116 using RT-PCR and WB. (G, H) Cell proliferation of SW620 (G) and HCT116 (H) in control and knockdown groups was detected using CCK8 assay. (I, J) Cell migration of SW620 (I) and HCT116 (J) in control and knockdown groups was detected using wound healing assay. (K, L) Cell invasion of SW620 (K) and HCT116 (L) in control and knockdown groups was detected using transwell assay. The scale bars on the lower right in (I–L) are 200 µm. *P < 0.05, **P < 0.01 and ***P < 0.001. NS, not significant difference. N=3.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9928961/&orig_host=www.ncbi.nlm.nih.gov'>36816947</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10035-2-IHC-anti-akr1b1-picoband-antibody.jpgAnti-AKR1B1 Antibody Picoband&reg; IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). <br> AKR1B1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10035-3-IHC-anti-akr1b1-picoband-antibody.jpgAnti-AKR1B1 Antibody Picoband&reg; IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). <br> AKR1B1 was detected in paraffin-embedded section of rat adrenal gland tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10035-4-IHC-anti-akr1b1-picoband-antibody.jpgAnti-AKR1B1 Antibody Picoband&reg; IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). <br> AKR1B1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10035-5.pngAnti-AKR1B1 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-AKR1B1 antibody (PB10035). <br> Overlay histogram showing U20S cells stained with PB10035 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1B1 Antibody (PB10035&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akr1c1-c2-picoband-trade-antibody-pb10036-boster.html2026-03-24T05:05:51+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-fonc-11-677678-g002.jpgAnti-AKR1C1/C2 Antibody Picoband&reg;cDNA array analysis identified AKR1C1 as a potential target of avasimibe. (A) RBE cells were treated with avasimibe at the concentration of 20 µM for 24 and 48 hours and subjected to cDNA array analysis. Cluster of changed genes in cell proliferation was presented. (B) The level of AKR1C1 mRNA and protein was detected by RT-PCR and western blotting in RBE cells when treated with avasimibe (20µM) for 48 hours. (C) The expression of AKR1C1 and PCNA was detected by IHC on the resected xenografts. IHC, ×200. *P < 0.05, **P < 0.01. ‘long scores of AKR1C1’ means AKR1C1 staining score. IHC, immunohistochemistry.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.677678/full'>34127944</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-fonc-11-677678-g003.jpgAnti-AKR1C1/C2 Antibody Picoband&reg;The oncogenic role of AKR1C1 in cholangiocarcinoma. (A) Representative images of negative staining of AKR1C1 in noncancerous tissues (N) and high expression of AKR1C1 in CCA (T). IHC,×200 for small pictures and×40 for large pictures. *P < 0.05. (B) Patients with AKR1C1 expression had a shorter time to recurrence (B1) and a worse overall survival (B2) than those without AKR1C1 expression. (C, D) QBC939 (C) and RBE (D) cells were transfected with AKR1C1-shRNA for 48 hours and the level of AKR1C1 mRNA was detected by RT-PCR. CCK8 assay was used to measure the cell viability of both cell lines. **P < 0.01, ****P < 0.0001. (E) QBC939 and RBE cells were treated with avasimibe with or without exogenous AKR1C1 plasmid for 48 hours and CCK8 was used to detect the changes of cell viability. IHC, immunohistochemistry; CCK8, Cell Counting Kit-8; Vec, vector.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.677678/full'>34127944</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-fonc-11-677678-g004.jpgAnti-AKR1C1/C2 Antibody Picoband&reg;AKR1C1 is regulated by FoxM1 in cholangiocarcinoma. (A) RBE cells were treated with avasimibe (20 µM) for 24 and 48 hours and subjected to cDNA array analysis. Cluster of changed genes in regulation of transcription was presented. (B) The level of FoxM1 mRNA and protein was detected by RT-PCR and western blotting in RBE cells when treated with avasimibe (20µM) for 48 hours. (C) RBE cells were transfected with FoxM1-shRNA for 48 hours and the levels of FoxM1 and AKR1C1 mRNA and proteins were detected by RT-PCR and western blotting. (D) RBE cells were transfected with FoxM1 plasmid for 48 hours and the levels of FoxM1 and AKR1C1 mRNA and proteins were detected by RT-PCR and western blotting. *P < 0.05, ****P < 0.0001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.677678/full'>34127944</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-fonc-11-677678-g005.jpgAnti-AKR1C1/C2 Antibody Picoband&reg;AKR1C1 is a direct transcriptional target of FoxM1. (A) Diagram shows the sequence and position of five putative FoxM1-binding elements in the AKR1C1 promoter. TSS, transcriptional start site; WT, wild type; Mut, mutant type. (B) Left panel, RBE cells were cotransfected with the AKR1C1 promoter reporter, pRL-TK, and pcDNA3.1-FoxM1 or pcDNA 3.1; right panel, RBE cells were cotransfected with the AKR1C1 promoter reporter, pRL-TK, and FoxM1-shRNA or shcontrol (50 nM). 36 hours after transfection, the cells were collected, and the relative AKR1C1 promoter activities were measured. The assay was repeated three times independently. ***P < 0.001. (C) Reporter plasmids harboring the wild-type AKR1C1 promoter or the corresponding mutant promoter in the FoxM1-binding sites were transfected into RBE cells, and the relative promoter activities were measured as above. (D) The chromatin immunoprecipitation (ChIP) assay results show the in vivo binding of FoxM1 to the AKR1C1 promoter. QBC939 cell lysis was immunoprecipitated using an anti-FoxM1 antibody or immunoglobulin G The resulting samples were subjected to RT-PCR using the site-specific primers.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.677678/full'>34127944</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-fonc-11-677678-g006.jpgAnti-AKR1C1/C2 Antibody Picoband&reg;Avasimibe inhibits cholangiocarcinoma cells proliferation via targeting AKR1C1 and FoxM1. (A, B) RBE cells (A) and QBC939 cells (B) were treated with avasimibe or DMSO, then transfected with FoxM1 or control vector, along with the transfection with AKR1C1 shRNA or shNT. 48 h after transfection, cell viability was analyzed by CCK8 assay. Data are from three independent assays. *P < 0.05, **P < 0.01, ***P < 0.001. (C) Left panel, the expression of FoxM1 and AKR1C1 was detected by IHC on the resected xenografts. IHC, ×400. Right panel, diagram showing the different expression of FoxM1 and AKR1C1 in these samples when treated with avasimibe. (D) The representative images of FoxM1 and AKR1C1 expressions and their correlations determined by Spearman’s correlation test. r, Spearman correlation coefficient; IHC, ×40 or ×200.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://www.frontiersin.org/journals/oncology/articles/10.3389/fonc.2021.677678/full'>34127944</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-aldh7a1-primary-antibodies-wb-testing-1.jpgAnti-AKR1C1/C2 Antibody Picoband&reg; Western blot analysis of AKR1C1/C2 using anti-AKR1C1/C2 antibody (PB10036). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human HCCP tissue lysates, <br> Lane 4: rat liver tissue lysates, <br> Lane 5: rat kidney tissue lysates, <br> Lane 6: mosue liver tissue lysates, <br> Lane 7: mosue kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKR1C1/C2 antigen affinity purified polyclonal antibody (Catalog # PB10036) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKR1C1/C2 at approximately 37 kDa. The expected band size for AKR1C1/C2 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-akr1c1-c2-primary-antibodies-if-testing-2.jpgAnti-AKR1C1/C2 Antibody Picoband&reg; IF analysis of AKR1C1/C2 using anti-AKR1C1/C2 antibody (PB10036). <br> AKR1C1/C2 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AKR1C1/C2 Antibody (PB10036) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10036-akr1c1-c2-primary-antibodies-fcm-testing-3_1.jpgAnti-AKR1C1/C2 Antibody Picoband&reg; Flow Cytometry analysis of HepG2 cells using anti-AKR1C1/C2 antibody (PB10036). <br>Overlay histogram showing HepG2 cells stained with PB10036 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AKR1C1/C2 Antibody (PB10036, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh1b1-picoband-trade-antibody-pb10037-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-wb-testing-1.jpgAnti-ALDH1B1 Antibody Picoband&reg; Western blot analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human A431 whole cell lysates, <br> Lane 4: human A549 whole cell lysates, <br> Lane 5: human U20S whole cell lysates, <br> Lane 6: human Hela whole cell lysates, <br> Lane 7: monkey COS-7 whole cell lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1B1 antigen affinity purified polyclonal antibody (Catalog # PB10037) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1B1 at approximately 55 kDa. The expected band size for ALDH1B1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-wb-testing-2.jpgAnti-ALDH1B1 Antibody Picoband&reg; Western blot analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat testis tissue lysates, <br> Lane 3: rat RH35 whole cell lysates, <br> Lane 4: mouse brain tissue lysates, <br> Lane 5: mouse liver tissue lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1B1 antigen affinity purified polyclonal antibody (Catalog # PB10037) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1B1 at approximately 55 kDa. The expected band size for ALDH1B1 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-ihc-testing-3.jpgAnti-ALDH1B1 Antibody Picoband&reg; IHC analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-ihc-testing-4.jpgAnti-ALDH1B1 Antibody Picoband&reg; IHC analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-ihc-testing-5.jpgAnti-ALDH1B1 Antibody Picoband&reg; IHC analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-ihc-testing-6.jpgAnti-ALDH1B1 Antibody Picoband&reg; IHC analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-ihc-testing-7.jpgAnti-ALDH1B1 Antibody Picoband&reg; IHC analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-fcm-testing-11.jpgAnti-ALDH1B1 Antibody Picoband&reg; Flow Cytometry analysis of HEL cells using anti-ALDH1B1 antibody (PB10037). <br>Overlay histogram showing HEL cells stained with PB10037 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH1B1 Antibody (PB10037, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-ihc-testing-8.jpgAnti-ALDH1B1 Antibody Picoband&reg; IHC analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-ihc-testing-9.jpgAnti-ALDH1B1 Antibody Picoband&reg; IHC analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10037-aldh1b1-primary-antibodies-if-testing-10.jpgAnti-ALDH1B1 Antibody Picoband&reg; IF analysis of ALDH1B1 using anti-ALDH1B1 antibody (PB10037). <br> ALDH1B1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ALDH1B1 Antibody (PB10037) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aldh7a1-picoband-trade-antibody-pb10038-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10038-aldh7a1-primary-antibodies-wb-testing-1.jpgAnti-ALDH7A1 Antibody Picoband&reg; Western blot analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HCCP tissue lysates, <br> Lane 2: rat liver tissue lysates, <br> Lane 3: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH7A1 antigen affinity purified polyclonal antibody (Catalog # PB10038) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH7A1 at approximately 55 kDa. The expected band size for ALDH7A1 is at 55 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10038-2-IHC-anti-aldh7a1-picoband-antibody.jpgAnti-ALDH7A1 Antibody Picoband&reg; IHC analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038). <br> ALDH7A1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10038-3-IHC-anti-aldh7a1-picoband-antibody.jpgAnti-ALDH7A1 Antibody Picoband&reg; IHC analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038). <br> ALDH7A1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10038-4.jpgAnti-ALDH7A1 Antibody Picoband&reg; IF analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038).<br> ALDH7A1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10038-5.pngAnti-ALDH7A1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ALDH7A1 antibody (PB10038). <br> Overlay histogram showing A431 cells stained with PB10038 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ALDH7A1 Antibody (PB10038&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB10038-6.jpgAnti-ALDH7A1 Antibody Picoband&reg; IF analysis of ALDH7A1 using anti-ALDH7A1 antibody (PB10038).<br> ALDH7A1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ALDH7A1 Antibody (PB10038) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-amfr-picoband-trade-antibody-pb10039-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10039-amfr-primary-antibodies-wb-testing-1.jpgAnti-AMFR Antibody Picoband&reg; Western blot analysis of AMFR using anti-AMFR antibody (PB10039). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human Hela whole cell lysates, <br> Lane 3: human Daudi whole cell lysates, <br> Lane 4: rat kidney tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMFR antigen affinity purified polyclonal antibody (Catalog # PB10039) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMFR at approximately 73 kDa. The expected band size for AMFR is at 73 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10039-2-IHC-anti-amfr-gp78-picoband-antibody.jpgAnti-AMFR Antibody Picoband&reg; IHC analysis of AMFR using anti-AMFR antibody (PB10039). <br> AMFR was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-AMFR Antibody (PB10039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10039-3.jpgAnti-AMFR Antibody Picoband&reg; IHC analysis of AMFR using anti-AMFR antibody (PB10039). <br> AMFR was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AMFR Antibody (PB10039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10039-4.jpgAnti-AMFR Antibody Picoband&reg; IHC analysis of AMFR using anti-AMFR antibody (PB10039). <br> AMFR was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AMFR Antibody (PB10039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10039-5.jpgAnti-AMFR Antibody Picoband&reg; Flow Cytometry analysis of SiHa cells using anti-AMFR antibody (PB10039). <br> Overlay histogram showing SiHa cells stained with PB10039 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMFR Antibody (PB10039&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10039-6.jpgAnti-AMFR Antibody Picoband&reg; Flow Cytometry analysis of U87 cells using anti-AMFR antibody (PB10039). <br> Overlay histogram showing U87 cells stained with PB10039 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AMFR Antibody (PB10039&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10039-amfr-primary-antibodies-if-testing-7.jpgAnti-AMFR Antibody Picoband&reg; IF analysis of AMFR using anti-AMFR antibody (PB10039). <br> AMFR was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AMFR Antibody (PB10039) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-abp1-picoband-trade-antibody-pb10040-boster.html2026-03-25T05:22:42+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10040-aoc1-primary-antibodies-wb-testing-1.jpgAnti-ABP1/AOC1 Antibody Picoband&reg; Western blot analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (PB10040). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human HK-2 whole cell lysates, <br> Lane 3: monkey COS-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABP1/AOC1 antigen affinity purified polyclonal antibody (Catalog # PB10040) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABP1/AOC1 at approximately 85 kDa. The expected band size for ABP1/AOC1 is at 85 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10040-aoc1-primary-antibodies-ihc-testing-2.jpgAnti-ABP1/AOC1 Antibody Picoband&reg; IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (PB10040). <br> ABP1/AOC1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABP1/AOC1 Antibody (PB10040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10040-aoc1-primary-antibodies-ihc-testing-3.jpgAnti-ABP1/AOC1 Antibody Picoband&reg; IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (PB10040). <br> ABP1/AOC1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABP1/AOC1 Antibody (PB10040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10040-aoc1-primary-antibodies-ihc-testing-4.jpgAnti-ABP1/AOC1 Antibody Picoband&reg; IHC analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (PB10040). <br> ABP1/AOC1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ABP1/AOC1 Antibody (PB10040) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10040-aoc1-primary-antibodies-if-testing-5.jpgAnti-ABP1/AOC1 Antibody Picoband&reg; IF analysis of ABP1/AOC1 using anti-ABP1/AOC1 antibody (PB10040). <br> ABP1/AOC1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ABP1/AOC1 Antibody (PB10040) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apc2-picoband-trade-antibody-pb10041-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10041-1-WB-anti-apc2-picoband-antibody.jpgAnti-APC2 Antibody Picoband&reg; Western blot analysis of APC2 using anti-APC2 antibody (PB10041). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APC2 antigen affinity purified polyclonal antibody (Catalog # PB10041) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APC2 at approximately 94 kDa. The expected band size for APC2 is at 94 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-apolipoprotein-b-picoband-trade-antibody-pb10042-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10042-2-IHC-anti-apolipoprotein-b-picoband-antibody.jpgAnti-Apolipoprotein B/APOB Antibody Picoband&reg; IHC analysis of Apolipoprotein CIII using anti-Apolipoprotein CIII antibody (PB10042). <br> Apolipoprotein CIII was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Apolipoprotein CIII Antibody (PB10042) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10042-1-WB-anti-apolipoprotein-b-picoband-antibody.jpgAnti-Apolipoprotein B/APOB Antibody Picoband&reg; Western blot analysis of Apolipoprotein CIII using anti-Apolipoprotein CIII antibody (PB10042). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apolipoprotein CIII antigen affinity purified polyclonal antibody (Catalog # PB10042) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apolipoprotein CIII at approximately 250 kDa. The expected band size for Apolipoprotein CIII is at 516 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-aqp11-picoband-trade-antibody-pb10044-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10044-1-WB-anti-aqp11-aquaporin-11-picoband-antibody.jpgAnti-AQP11 Antibody Picoband&reg; Western blot analysis of AQP11 using anti-AQP11 antibody (PB10044). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: mouse brain tissue lysates, <br> Lane 3: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AQP11 antigen affinity purified polyclonal antibody (Catalog # PB10044) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AQP11 at approximately 30 kDa. The expected band size for AQP11 is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-arhgef1-picoband-trade-antibody-pb10045-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10045-1-WB-anti-arhgef1-lsc-picoband-antibody.jpgAnti-ARHGEF1 Antibody Picoband&reg; Western blot analysis of ARHGEF1 using anti-ARHGEF1 antibody (PB10045). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates, <br> Lane 2: HELA whole cell lysates, <br> Lane 3: JURKAT whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGEF1 antigen affinity purified polyclonal antibody (Catalog # PB10045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGEF1 at approximately 120 kDa. The expected band size for ARHGEF1 is at 102 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10045-2-IHC-anti-arhgef1-lsc-picoband-antibody.jpgAnti-ARHGEF1 Antibody Picoband&reg; IHC analysis of ARHGEF1 using anti-ARHGEF1 antibody (PB10045). <br> ARHGEF1 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ARHGEF1 Antibody (PB10045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10045-3-IHC-anti-arhgef1-lsc-picoband-antibody.jpgAnti-ARHGEF1 Antibody Picoband&reg; IHC analysis of ARHGEF1 using anti-ARHGEF1 antibody (PB10045). <br> ARHGEF1 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ARHGEF1 Antibody (PB10045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10045-4.jpgAnti-ARHGEF1 Antibody Picoband&reg; IF analysis of ARHGEF1 using anti-ARHGEF1 antibody (PB10045). <br> ARHGEF1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ARHGEF1 Antibody (PB10045) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10045-5.jpgAnti-ARHGEF1 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-ARHGEF1 antibody (PB10045).<br>Overlay histogram showing A431 cells stained with PB10045 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARHGEF1 Antibody (PB10045,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-accn1-picoband-trade-antibody-pb10046-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10046-1-WB-anti-accn1-picoband-antibody.jpgAnti-ACCN1/ASIC2 Antibody Picoband&reg; Western blot analysis of ACCN1 using anti-ACCN1 antibody (PB10046). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates, <br> Lane 2: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACCN1 antigen affinity purified polyclonal antibody (Catalog # PB10046) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACCN1 at approximately 65 kDa. The expected band size for ACCN1 is at 58 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bdkrb2-picoband-trade-antibody-pb10047-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb9396-bdkrb2-primary-antibodies-wb-testing-1.jpgAnti-BDKRB2 Antibody Picoband&reg;Western blot analysis of BDKRB2 using anti-BDKRB2 antibody (PB9396). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hacat whole cell lysates,<br> Lane 2: human A431 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat C6 whole cell lysates,<br> Lane 6: mouse Neuro-2a whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDKRB2 antigen affinity purified polyclonal antibody (PB9396) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for BDKRB2 at approximately 78 kDa. The expected band size for BDKRB2 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bmp15-picoband-trade-antibody-pb10048-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10048-1-WB-anti-bmp15-gdf-9b-picoband-antibody.jpgAnti-BMP15 Antibody Picoband&reg; Western blot analysis of BMP15 using anti-BMP15 antibody (PB10048). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates, <br> Lane 2: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP15 antigen affinity purified polyclonal antibody (Catalog # PB10048) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP15 at approximately 56 kDa. The expected band size for BMP15 is at 45 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ca3-picoband-trade-antibody-pb10049-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10049-ca3-primary-antibodies-wb-testing-1.jpgAnti-Carbonic Anhydrase III/CA3 Antibody Picoband&reg;Western blot analysis of CA3 using anti-CA3 antibody (PB10049). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates,<br> Lane 2: rat fat tissue lysates,<br> Lane 3: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CA3 antigen affinity purified polyclonal antibody (PB10049) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CA3 at approximately 30 kDa. The expected band size for CA3 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10049-2-IHC-anti-ca3-carbonic-anhydrase-iii-picoband-antibody.jpgAnti-Carbonic Anhydrase III/CA3 Antibody Picoband&reg; IHC analysis of CA3 using anti-CA3 antibody (PB10049). <br> CA3 was detected in a paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CA3 Antibody (PB10049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10049-3-IHC-anti-ca3-carbonic-anhydrase-iii-picoband-antibody.jpgAnti-Carbonic Anhydrase III/CA3 Antibody Picoband&reg; IHC analysis of CA3 using anti-CA3 antibody (PB10049). <br> CA3 was detected in a paraffin-embedded section of rat skeletal muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CA3 Antibody (PB10049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10049-4-IHC-anti-ca3-carbonic-anhydrase-iii-picoband-antibody.jpgAnti-Carbonic Anhydrase III/CA3 Antibody Picoband&reg; IHC analysis of CA3 using anti-CA3 antibody (PB10049). <br> CA3 was detected in a paraffin-embedded section of human osteosarcoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CA3 Antibody (PB10049) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ccdc6-picoband-trade-antibody-pb10050-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10050-1-WB-anti-ccdc6-picoband-antibody.jpgAnti-CCDC6 Antibody Picoband&reg; Western blot analysis of CCDC6 using anti-CCDC6 antibody (PB10050). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates, <br> Lane 2: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCDC6 antigen affinity purified polyclonal antibody (Catalog # PB10050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCDC6 at approximately 66 kDa. The expected band size for CCDC6 is at 53 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10050-ccdc6-primary-antibodies-if-testing-2.jpgAnti-CCDC6 Antibody Picoband&reg; IF analysis of CCDC6 using anti-CCDC6 antibody (PB10050). <br> CCDC6 was detected in immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CCDC6 Antibody (PB10050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10050-ccdc6-primary-antibodies-fcm-testing-3.pngAnti-CCDC6 Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-CCDC6 antibody (PB10050). <br>Overlay histogram showing CACO-2 cells stained with PB10050 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCDC6 Antibody (PB10050, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd40-tnfrsf5-picoband-trade-antibody-pb10051-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10051-cd40-primary-antibodies-wb-testing-1_1.jpgAnti-CD40 Antibody Picoband&reg; Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Raji whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10051) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 43 kDa. The expected band size for CD40/TNFRSF5 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10051-cd40-primary-antibodies-ihc-testing-2.jpgAnti-CD40 Antibody Picoband&reg; IHC analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10051). <br> CD40/TNFRSF5 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD40/TNFRSF5 Antibody (PB10051) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10051-cd40-primary-antibodies-fcm-testing-3.jpgAnti-CD40 Antibody Picoband&reg; Flow Cytometry analysis of human PBMC cells using anti-CD40/TNFRSF5 antibody (PB10051). <br>Overlay histogram showing human PBMC cells stained with PB10051 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40/TNFRSF5 Antibody (PB10051, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cd40-tnfrsf5-trade-antibody-pb10052-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10052-2-IHC-anti-cd40-tnfrsf5-picoband-antibody.jpgAnti-CD40 Antibody Picoband&reg; IHC analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10052). <br> CD40/TNFRSF5 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD40/TNFRSF5 Antibody (PB10052) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10052-1-WB-anti-cd40-tnfrsf5-picoband-antibody.jpgAnti-CD40 Antibody Picoband&reg; Western blot analysis of CD40/TNFRSF5 using anti-CD40/TNFRSF5 antibody (PB10052). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40/TNFRSF5 antigen affinity purified polyclonal antibody (Catalog # PB10052) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40/TNFRSF5 at approximately 40 kDa. The expected band size for CD40/TNFRSF5 is at 32 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10052-cd40-primary-antibodies-fc-testing-3.pngAnti-CD40 Antibody Picoband&reg;3. Flow Cytometry analysis of U937 cells using anti-CD40/TNFRSF5 antibody (PB10052).<br>Overlay histogram showing U937 cells stained with PB10052 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD40/TNFRSF5 Antibody (PB10052&#44;1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cathepsin-g-trade-antibody-pb10053-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10053-ctsg-primary-antibodies-wb-testing-1.jpgAnti-Cathepsin G/CTSG Antibody Picoband&reg; Western blot analysis of Cathepsin G/CTSG using anti-Cathepsin G/CTSG antibody (PB10053). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human THP-1 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin G/CTSG antigen affinity purified polyclonal antibody (Catalog # PB10053) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin G/CTSG at approximately 29 kDa. The expected band size for Cathepsin G/CTSG is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10053-ctsg-primary-antibodies-ihc-testing-2.jpgAnti-Cathepsin G/CTSG Antibody Picoband&reg; IHC analysis of Cathepsin G/CTSG using anti-Cathepsin G/CTSG antibody (PB10053). <br> Cathepsin G/CTSG was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cathepsin G/CTSG Antibody (PB10053) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cxcl16-picoband-trade-antibody-pb10054-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10054-1-WB-anti-cxcl16-picoband-antibody.jpgAnti-CXCL16 Antibody Picoband&reg; Western blot analysis of CXCL16 using anti-CXCL16 antibody (PB10054). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: CEM whole cell lysates, <br> Lane 2: A549 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL16 antigen affinity purified polyclonal antibody (Catalog # PB10054) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CXCL16 at approximately 35 kDa. The expected band size for CXCL16 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10054-cxcl16-primary-antibodies-elisa-testing-2.jpgAnti-CXCL16 Antibody Picoband&reg; Sandwich ELISA - Recombinant human cxcl16 protein standard curve.<br> Use in combination with reagents from Human cxcl16 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0741). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyp3a4-trade-antibody-pb10055-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10055-1_1-WB-anti-cyp3a4-cytochrome-p450-3a4-picoband-antibody.jpgAnti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband&reg; Western blot analysis of CYP3A4 using anti-CYP3A4 antibody (PB10055). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP3A4 antigen affinity purified polyclonal antibody (Catalog # PB10055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP3A4 at approximately 57 kDa. The expected band size for CYP3A4 is at 57 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10055-2-IHC-anti-cyp3a4-cytochrome-p450-3a4-picoband-antibody.jpgAnti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband&reg; IHC analysis of CYP3A4 using anti-CYP3A4 antibody (PB10055). <br> CYP3A4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP3A4 Antibody (PB10055) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-dhodh-trade-antibody-pb10056-boster.html2026-03-24T05:05:52+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10056-dhodh-primary-antibodies-wb-testing-1.jpgAnti-DHODH Antibody Picoband&reg; Western blot analysis of DHODH using anti-DHODH antibody (PB10056). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human HepG2 whole cell lysates, <br> Lane 4: human MCF-7 whole cell lysates, <br> Lane 5: rat testis tissue lysates, <br> Lane 6: rat liver tissue lysates, <br> Lane 7: mouse testis tissue lysates, <br> Lane 8: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DHODH antigen affinity purified polyclonal antibody (PB10056) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DHODH at approximately 43 kDa. The expected band size for DHODH is at 43 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10056-2-IHC-anti-dhodh-picoband-antibody.jpgAnti-DHODH Antibody Picoband&reg; IHC analysis of DHODH using anti-DHODH antibody (PB10056). <br> DHODH was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DHODH Antibody (PB10056) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10056-3-IHC-anti-dhodh-picoband-antibody.jpgAnti-DHODH Antibody Picoband&reg; IHC analysis of DHODH using anti-DHODH antibody (PB10056). <br> DHODH was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DHODH Antibody (PB10056) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10056-4-IHC-anti-dhodh-picoband-antibody.jpgAnti-DHODH Antibody Picoband&reg; IHC analysis of DHODH using anti-DHODH antibody (PB10056). <br> DHODH was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-DHODH Antibody (PB10056) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-e2f4-trade-antibody-pb10057-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10057-1-WB-anti-e2f4-picoband-antibody.jpgAnti-E2F4 Antibody Picoband&reg; Western blot analysis of E2F4 using anti-E2F4 antibody (PB10057). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates, <br> Lane 2: U20S whole cell lysates, <br> Lane 3: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E2F4 antigen affinity purified polyclonal antibody (Catalog # PB10057) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for E2F4 at approximately 44 kDa, 60 kDa. The expected band size for E2F4 is at 44 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10057-2-IHC-anti-e2f4-picoband-antibody.jpgAnti-E2F4 Antibody Picoband&reg; IHC analysis of E2F4 using anti-E2F4 antibody (PB10057). <br> E2F4 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-E2F4 Antibody (PB10057) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10057-3-IHC-anti-e2f4-picoband-antibody.jpgAnti-E2F4 Antibody Picoband&reg; IHC analysis of E2F4 using anti-E2F4 antibody (PB10057). <br> E2F4 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-E2F4 Antibody (PB10057) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10057-4-IHC-anti-e2f4-picoband-antibody.jpgAnti-E2F4 Antibody Picoband&reg; IHC analysis of E2F4 using anti-E2F4 antibody (PB10057). <br> E2F4 was detected in a paraffin-embedded section of human intetsinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-E2F4 Antibody (PB10057) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-elastas-elane-ela2-trade-antibody-pb10058-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-41420_2024_2156_fig2_html.pngAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;Neutrophil infiltration and NETs release within AAA lesions. A IF staining for neutrophil marker (Ly6G) was performed on tissue sections of the mouse abdominal aorta. B Hyalinization and IF labeling of the whole abdominal aorta and 3D tissue imaging were acquired with light-sheet microscopy. C – E Quantification of NETs markers (ELANE and MPO) in mouse abdominal aorta by western blotting ( n = 3). ** P < 0.01, **** P < 0.0001, indicating statistical significance between the two groups. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41420-024-02156-3'>39237520</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0001.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;Successful establishment of CI-AKI model in mice. ( A ) HE staining of kidney (magnification 400×). ( B ) Quantification of renal tubular injury scores (n=6). ( C ) The SCr levels in different contrast dose groups (n=6). ( D ) The SCr levels in mice at different time periods after contrast injection (n=6). ( E ) Transmission electron micrographs of kidney tissues in mice. Among them, renal tubular epithelial cells (RTECs), vascular endothelial cells (VECs), nucleus (N), mitochondria (Mi), rough endoplasmic reticulum (RER), peduncle: yellow arrow (↑), mitochondrial swelling: red arrow (↑), basement membrane: green arrow (↑), vacuole: blue arrow (↑), autophagy: purple arrow (↑). Magnification 20,000×. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, indicating statistically significant data between groups; “ns” indicates no statistical significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0002.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;NETs are present in the kidneys of CI-AKI mice and correlate with the degree of kidney injury. ( A ) IF of kidney sections. Among them, blue, DAPI, nucleus; red, Ly6G, neutrophil surface marker; green, CitH3, NETs-specific marker. Magnification 4000×. ( B ) ELISA method to detect the expression level of serum MPO in mice (n=6). ( C ) Correlation analysis of serum MPO levels with SCr in CI-AKI mice (n=12). ( D – F ) IF double staining and semiquantitative analysis of renal paraffin sections (n=6). Among them, blue, DAPI; red, Ly6G; green, CitH3. Magnification 630×. ( G and H ) Histochemical staining of kidney paraffin sections for MPO (n=6). Magnification 1000×. * VS control group, *P < 0.05, ***P < 0.001, ****P < 0.0001, indicating statistically significant data between groups; # VS 24 h-L group, # P < 0.05, ## P < 0.01, ### P < 0.001, indicating statistically significant data between groups; ns indicates no statistical significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0003.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;Expression of NETs protein and mRNA in the kidney of CI-AKI mice. ( A – C ) Expression and quantification of PADI4 and CitH3 in mice kidney by Western blot (n=3). ( D and E ) Detection of PADI4, MPO and ELANE, and mRNA expression of TLR2 and TLR4 by qRT-PCR (n=3). ( F and G ) Expression levels of inflammatory factors TNF-α and IL-6 in serum of mice (n=3). * VS control group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, indicating statistically significant data between groups; # VS 24 h-L group, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001, indicating statistically significant data between groups; ns indicates no statistical significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0004.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;NETs accumulate in the glomeruli and PTC of CI-AKI mice. ( A ) IF co-staining: CD31, red, vascular endothelial cell marker; MPO, green, NETs marker. Magnification 1300×. ( B ) IF co-staining: Ly6G, red; CitH3, green. Magnification 1300×. ( C and D ) Neutrophil residency and NETs release in PTC. Left side magnification 1300×, right side magnification 4000×.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0005.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;GSK484 or DNase I reduces the accumulation of NETs in CI-AKI mice. ( A – C ) IF co-staining results and semi-quantitative analysis of Ly6G and CitH3 in mouse kidney sections (n=6). Magnification 630×. ( D and E ) Immunohistochemical staining and semi-quantitative analysis of MPO in mouse kidney sections (n=6). Magnification 1000×. ( F ) The expression of MPO in mouse serum (n=3). ( G and H ) Quantification of protein in mouse kidney ELANE (n=3). * VS control group, ***P < 0.001, ****P < 0.0001, indicates statistically significant data between groups; # VS CI-AKI group, # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001, indicates statistically significant data between groups.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0006.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;GSK484 or DNase I attenuates kidney injury in mice. ( A ) HE and PAS staining of kidney tissue (magnification 400×). ( B ) Renal tubular injury score (n=6). ( C ) Expression of SCr in mice (n=6). ( D and E ) Expression levels of serum inflammatory factors TNF-α and IL-6 in mice (n=3). * VS control group, **P < 0.01, ****P < 0.0001, indicating statistically significant data between groups; # VS CI-AKI group, # P < 0.05, ## P < 0.01, ### P < 0.001, indicating statistically significant data between groups; ns indicates no statistical significance.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0007.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;Reduction of NETs levels alleviates endothelial cell injury in glomeruli and PTC. ( A – C ) IF co-staining and semi-quantitative analysis of CD31 and CitH3 in kidney sections (n=6). Magnification 630×. ( D and E ) Immunohistochemical staining and semi-quantitative analysis of PV-1 in kidney sections (n=6). Magnification 1300×. * VS control group, **P < 0.01, ****P < 0.0001, indicating statistically significant data between groups; # VS CI-AKI group, # P < 0.05, ## P < 0.01, ### P < 0.001, indicating statistically significant data between groups.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-jir-16-5629-g0008.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg;Reducing the level of NETs reduces pyroptosis. ( A – C ) Quantitative analysis of GSDMD and N-GSDMD protein in kidney (n=3). ( D and E ) IF double staining and semi-quantitative analysis of Caspase-1 and IL-1β in kidney tissue sections (n=3). * VS control group, ***P < 0.001, ****P < 0.0001, indicating statistically significant data between groups; # VS CI-AKI group, # P < 0.05, ### P < 0.001, #### P < 0.0001, indicating statistically significant data between groups. Magnification 630×.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC10693253/&orig_host=www.ncbi.nlm.nih.gov'>38046404</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-ela2-primary-antibodies-wb-testing-1.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg; Western blot analysis of Elastase/ELANE/ELA2 using anti-Elastase/ELANE/ELA2 antibody (PB10058). <br> Electrophoresis was performed on a 13% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse spleen tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Elastase/ELANE/ELA2 antigen affinity purified polyclonal antibody (PB10058) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Elastase/ELANE/ELA2 at approximately 29 kDa. The expected band size for Elastase/ELANE/ELA2 is at 29 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-ela2-primary-antibodies-ihc-testing-2.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg; IHC analysis of Elastase/ELANE/ELA2 using anti-Elastase/ELANE/ELA2 antibody (PB10058). <br> Elastase/ELANE/ELA2 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elastase/ELANE/ELA2 Antibody (PB10058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10058-ela2-primary-antibodies-ihc-testing-3.jpgAnti-Neutrophil Elastase/ELANE Antibody Picoband&reg; IHC analysis of Elastase/ELANE/ELA2 using anti-Elastase/ELANE/ELA2 antibody (PB10058). <br> Elastase/ELANE/ELA2 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Elastase/ELANE/ELA2 Antibody (PB10058) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-epcam-trade-antibody-pb10059-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10059-epcam-primary-antibodies-wb-testing-1_1.jpgAnti-EpCAM Antibody Picoband&reg; Western blot analysis of EPCAM using anti-EPCAM antibody (PB10059). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human CACO-2 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCAM antigen affinity purified polyclonal antibody (Catalog # PB10059) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCAM at approximately 35-40 kDa. The expected band size for EPCAM is at 35 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10059-epcam-primary-antibodies-ihc-testing-2.jpgAnti-EpCAM Antibody Picoband&reg; IHC analysis of EPCAM using anti-EPCAM antibody (PB10059). <br> EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPCAM Antibody (PB10059) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10059-epcam-primary-antibodies-ihc-testing-3.jpgAnti-EpCAM Antibody Picoband&reg; IHC analysis of EPCAM using anti-EPCAM antibody (PB10059). <br> EPCAM was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-EPCAM Antibody (PB10059) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10059-epcam-primary-antibodies-if-testing-4.jpgAnti-EpCAM Antibody Picoband&reg; IF analysis of EPCAM using anti-EPCAM antibody (PB10059). <br> EPCAM was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-EPCAM Antibody (PB10059) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10059-epcam-primary-antibodies-fcm-testing-5.jpgAnti-EpCAM Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-EPCAM antibody (PB10059). <br> Overlay histogram showing CACO-2 cells stained with PB10059 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-EPCAM Antibody (PB10059, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-csb-picoband-trade-antibody-pb10060-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10060-ercc6-primary-antibodies-wb-testing-1.jpgAnti-CSB/ERCC6 Antibody Picoband&reg; Western blot analysis of CSB using anti-CSB antibody (PB10060). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A431 whole cell lysates, <br> Lane 3: human Siha whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf19-picoband-trade-antibody-pb10061-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10061-1-WB-anti-fgf19-picoband-antibody.jpgAnti-FGF19 Antibody Picoband&reg; Western blot analysis of FGF19 using anti-FGF19 antibody (PB10061). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF19 antigen affinity purified polyclonal antibody (Catalog # PB10061) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF19 at approximately 24 kDa. The expected band size for FGF19 is at 24 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10061-fgf19-primary-antibodies-elisa-testing-2.jpgAnti-FGF19 Antibody Picoband&reg; Sandwich ELISA - Recombinant human fgf19 protein standard curve.<br> Use in combination with reagents from Human fgf19 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1160). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf19-picoband-trade-antibody-pb10062-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10062-fgf19-fgf15-primary-antibodies-ihc-testing-1_1.jpgAnti-Fgf19(Fgf15) Antibody IHC analysis of Fgf19(Fgf15) using anti-Fgf19(Fgf15) antibody (PB10062). <br> Fgf19(Fgf15) was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fgf19(Fgf15) Antibody (PB10062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10062-fgf19-fgf15-primary-antibodies-ihc-testing-2_1.jpgAnti-Fgf19(Fgf15) Antibody IHC analysis of Fgf19(Fgf15) using anti-Fgf19(Fgf15) antibody (PB10062). <br> Fgf19(Fgf15) was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fgf19(Fgf15) Antibody (PB10062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fgf21-picoband-trade-antibody-pb10063-boster.html2026-04-01T05:01:26+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10063-1-WB-anti-fgf21-picoband-antibody.jpgAnti-FGF21 Antibody Picoband&reg; Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: human placenta tissue lysates&#44; <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-ijcep0013-0220-f4.jpgAnti-FGF21 Antibody Picoband&reg;Effects of sappanwood ethyl acetate extract (SEAE) on mRNA and protein expression levels of SREBP-2 and FGF21 in the high-fat- and vitamin D3-induced atherosclerosis (AS) rat model. A. mRNA expression levels of SREBP-2 and FGF21 were determined by real-time RT-PCR. GAPDH was used as an internal control. B. Protein expression levels of SREBP-2 and FGF21 were determined by western blot. β-actin was used as an internal control. Data are shown as means ± standard deviations (SD) (n=3 each group). * P <0.05 vs. blank control; # P <0.05 vs. AS model; Δ P <0.05 vs. simvastatin.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC7061805/&orig_host=www.ncbi.nlm.nih.gov'>32211102</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-gnl-12-449f1.jpgAnti-FGF21 Antibody Picoband&reg;Analysis of serum FGF19 and FGF21 levels in patients with viral or alcoholic hepatitis. Serum levels of FGF19 (A) and FGF21 (B) were determined by enzyme-linked immunosorbent assay. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor. *p<0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6027831/&orig_host=www.ncbi.nlm.nih.gov'>29699061</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-gnl-12-449f2.jpgAnti-FGF21 Antibody Picoband&reg;Analysis of FGF19, FGF21, and β-Klotho expression and inflammatory markers in liver tissue. Expression levels of mRNA for FGF19 (A), FGF21 (B), β-Klotho (C), IL-1β (D), IL-6 (E), and TNF-α (F) were determined by real-time polymerase chain reaction. G1 (F0–F1, n=10), G2 (F2–F3, n=10), G3 (F4A–F4C, n=15). FGF, fibroblast growth factor; IL, interleukin; TNF, tumor necrosis factor. *p<0.05, † p<0.01.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6027831/&orig_host=www.ncbi.nlm.nih.gov'>29699061</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-gnl-12-449f4.jpgAnti-FGF21 Antibody Picoband&reg;Effects of IL-1β on β-Klotho and FGF21 in Huh-7 cells. Huh-7 cells were incubated with IL-1β for 6 hours. β-Klotho expression levels (A and G), protein kinase B (B), extracelluar signal-regulated kinases (C), JNK (D), IκBα (E), and FGF21 (F and G) were determined by immunoblotting. IL, interleukin; FGF, fibroblast growth factor; JNK, c-Jun N-terminal kinase.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6027831/&orig_host=www.ncbi.nlm.nih.gov'>29699061</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-gnl-12-449f5.jpgAnti-FGF21 Antibody Picoband&reg;Signaling pathways that inhibit β-Klotho and induce FGF21 by IL-1β in Huh-7 cells. Huh-7 cells were pretreated with an NF-κB inhibitor (Bay11-7082, A and E), JNK inhibitor (SP600125, B and F), protein kinase B inhibitor (LY294002, C and G), or extracelluar signal-regulated kinases inhibitor (PD98059, D and H) for 20 minutes and then treated with 10 ng/mL of IL-1β for 6 hours to detect β-Klotho and FGF21. (I) Huh-7 cells were pretreated with 1 μM NF-κB inhibitor Bay11-7082, after which β-Klotho and FGF21 levels were determined by immunoblotting. (J) Huh-7 cells were pretreated with 0.5 μM JNK inhibitor SP600125, after which β-Klotho and FGF21 levels were determined by immunoblotting. (K) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 1 μM NF-κB inhibitor Bay11-7082 in Huh-7 cells. (L) β-Klotho and FGF21 levels were assessed 6 hours after pretreatment with 0.5 μM JNK inhibitor SP600125 in Huh-7 cells. FGF, fibroblast growth factor; IL, interleukin; NF-κB, nuclear factor-κB; JNK, c-Jun N-terminal kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6027831/&orig_host=www.ncbi.nlm.nih.gov'>29699061</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-gnl-12-449f6.jpgAnti-FGF21 Antibody Picoband&reg;FGF21 inhibits IL-1β-induced growth retardation of hepatocytes. Huh-7 cells were incubated with 10 ng/mL of IL-1β (A), 500 ng/mL of FGF21 (B), or IL-1β+FGF21 (C), after which the expression of β-Klotho and PCNA was determined. (D) IL-1β-treated Huh-7 cells were incubated with or without FGF21 in a dose-dependent manner. Cell viability was measured by an methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. IL, interleukin; FGF, fibroblast growth factor; PCNA, proliferating cell nuclear antigen; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC6027831/&orig_host=www.ncbi.nlm.nih.gov'>29699061</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-2.jpgAnti-FGF21 Antibody Picoband&reg; Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: rat spleen tissue lysates&#44; <br> Lane 2: rat liver tissue lysates&#44; <br> Lane 3: rat RH35 whole cell lysates&#44; <br> Lane 4: rat brain tissue lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10063-3.jpgAnti-FGF21 Antibody Picoband&reg; Western blot analysis of FGF21 using anti-FGF21 antibody (PB10063). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: mouse RAW264.7 whole cell lysates. <br> After Electrophoresis&#44; proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF21 antigen affinity purified polyclonal antibody (Catalog # PB10063) at 0.5 μg/mL overnight at 4°C&#44; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF21 at approximately 22KD. The expected band size for FGF21 is at 22KD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fhl3-picoband-trade-antibody-pb10064-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10064-fhl3-primary-antibodies-wb-testing-1.jpgAnti-FHL3 Antibody Picoband&reg; Western blot analysis of FHL3 using anti-FHL3 antibody (PB10064). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human K562 whole cell lysates,<br> Lane 2: rat heart tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL3 antigen affinity purified polyclonal antibody (Catalog # PB10064) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL3 at approximately 31 kDa. The expected band size for FHL3 is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-fsh-receptor-picoband-trade-antibody-pb10065-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10065-1-WB-anti-fsh-receptor-picoband-antibody.jpgAnti-FSH Receptor/FSHR Antibody Picoband&reg; Western blot analysis of FSH Receptor using anti-FSH Receptor antibody (PB10065). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates， <br> Lane 2: rat ovary tissue lysates， <br> Lane 3: mouse testis tissue lysates， <br> Lane 4: mouse ovary tissue lysates, <br> Lane 5: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH Receptor antigen affinity purified polyclonal antibody (Catalog # PB10065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH Receptor at approximately 78 kDa. The expected band size for FSH Receptor is at 78 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-txnl2-picoband-trade-antibody-pb10066-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10066-txnl2-primary-antibodies-wb-testing-1.jpgAnti-TXNL2/GLRX3 Antibody Picoband&reg; Western blot analysis of TXNL2 using anti-TXNL2 antibody (PB10066). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Jurkat whole cell lysates,<br> Lane 2: rat testis tissue lysates,<br> Lane 3: mouse testis tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNL2 antigen affinity purified polyclonal antibody (Catalog # PB10066) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNL2 at approximately 37 kDa. The expected band size for TXNL2 is at 37 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gnb1-trade-antibody-pb10067-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10067-gnb1-primary-antibodies-wb-testing-1_1.jpgAnti-GNB1 Antibody Picoband&reg; Western blot analysis of GNB1 using anti-GNB1 antibody (PB10067). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human SiHa whole cell lysates,<br> Lane 4: rat brain tissue lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: mouse brain tissue lysates,<br> Lane 7: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNB1 antigen affinity purified polyclonal antibody (Catalog # PB10067) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for GNB1 at approximately 37 kDa. The expected band size for GNB1 is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10067-2_2-IHC-anti-gnb1-picoband-antibody.jpgAnti-GNB1 Antibody Picoband&reg; IHC analysis of GNB1 using anti-GNB1 antibody (PB10067). <br> GNB1 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GNB1 Antibody (PB10067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10067-3_1-IHC-anti-gnb1-picoband-antibody.jpgAnti-GNB1 Antibody Picoband&reg; IHC analysis of GNB1 using anti-GNB1 antibody (PB10067). <br> GNB1 was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GNB1 Antibody (PB10067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10067-4_2-IHC-anti-gnb1-picoband-antibody.jpgAnti-GNB1 Antibody Picoband&reg; IHC analysis of GNB1 using anti-GNB1 antibody (PB10067). <br> GNB1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GNB1 Antibody (PB10067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10067-5.jpgAnti-GNB1 Antibody Picoband&reg; IHC analysis of GNB1 using anti-GNB1 antibody (PB10067). <br> GNB1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GNB1 Antibody (PB10067) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10067-6.jpgAnti-GNB1 Antibody Picoband&reg; IF analysis of GNB1 using anti-GNB1 antibody (PB10067).<br> GNB1 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-GNB1 Antibody (PB10067) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10067-7.pngAnti-GNB1 Antibody Picoband&reg; Flow Cytometry analysis of U937 cells using anti-GNB1 antibody (PB10067). <br> Overlay histogram showing U937 cells stained with PB10067 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNB1 Antibody (PB10067&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gpi-picoband-trade-antibody-pb10068-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10068-gpi-primary-antibodies-wb-testing-1.jpgAnti-Glucose 6 phosphate isomerase/GPI Antibody Picoband&reg;Western blot analysis of GPI using anti-GPI antibody (PB10068). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SiHa whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human K562 whole cell lysates, <br> Lane 4: human A431 whole cell lysates, <br> Lane 5: rat lung tissue lysates, <br> Lane 6: rat heart tissue lysates, <br> Lane 7: mouse lung tissue lysates, <br> Lane 8: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPI antigen affinity purified polyclonal antibody (PB10068) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GPI at approximately 63 kDa. The expected band size for GPI is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10068-gpi-primary-antibodies-fcm-testing-1.jpgAnti-Glucose 6 phosphate isomerase/GPI Antibody Picoband&reg;Flow Cytometry analysis of K562 cells using anti-GPI antibody (PB10068). <br> Overlay histogram showing K562 cells stained with PB10068 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GPI Antibody (PB10068, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gpi-picoband-trade-antibody-pb10069-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10069-gpi-primary-antibodies-wb-testing-1.jpgAnti-Glucose 6 phosphate isomerase/GPI Antibody Picoband&reg; Western blot analysis of GPI using anti-GPI antibody (PB10069). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse HEPA1-6 whole cell lysates, <br> Lane 2: mouse NIH/3T3 whole cell lysates, <br> Lane 3: mouse spleen tissue lysates, <br> Lane 4: mouse kidney tissue lysates, <br> Lane 5: mouse thymus tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPI antigen affinity purified polyclonal antibody (Catalog # PB10069) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPI at approximately 64 kDa. The expected band size for GPI is at 63 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hltf-picoband-trade-antibody-pb10070-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10070-hltf-primary-antibodies-wb-testing-1.jpgAnti-HLTF Antibody Picoband&reg; Western blot analysis of HLTF using anti-HLTF antibody (PB10070). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human PANC-1 whole cell lysates,<br> Lane 3: human A549 whole cell lysates,<br> Lane 4: rat heart tissue lysates,<br> Lane 5: rat kidney tissue lysates,<br> Lane 6: mouse heart tissue lysates,<br> Lane 7: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLTF antigen affinity purified polyclonal antibody (Catalog # PB10070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLTF at approximately 114 kDa. The expected band size for HLTF is at 114 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10070-hltf-primary-antibodies-if-testing-2.jpgAnti-HLTF Antibody Picoband&reg; IF analysis of HLTF using anti-HLTF antibody (PB10070) and anti-Tubulin Alpha antibody (M03989-3).<br> HLTF was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HLTF Antibody (PB10070) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10070-hltf-primary-antibodies-fcm-testing-3.jpgAnti-HLTF Antibody Picoband&reg; Flow Cytometry analysis of CACO-2 cells using anti-HLTF antibody (PB10070). <br>Overlay histogram showing CACO-2 cells stained with PB10070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLTF Antibody (PB10070, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hprt-picoband-trade-antibody-pb10071-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10071-hprt1-primary-antibodies-wb-testing-1.jpgAnti-HPRT/HPRT1 Antibody Picoband&reg; Western blot analysis of HPRT/HPRT1 using anti-HPRT/HPRT1 antibody (PB10071). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates,<br> Lane 2: human Colo320 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human 293T whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat spleen tissue lysates,<br> Lane 7: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT/HPRT1 antigen affinity purified polyclonal antibody (Catalog # PB10071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HPRT/HPRT1 at approximately 25 kDa. The expected band size for HPRT/HPRT1 is at 25 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-insulin-receptor-picoband-trade-antibody-pb10072-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10072-1-WB-anti-insulin-receptor-picoband-antibody.jpgAnti-Insulin Receptor/INSR Antibody Picoband&reg; Western blot analysis of Insulin Receptor using anti-Insulin Receptor antibody (PB10072). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: HEPG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Insulin Receptor antigen affinity purified polyclonal antibody (Catalog # PB10072) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Insulin Receptor at approximately 155 kDa. The expected band size for Insulin Receptor is at 156 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-kdm5b-picoband-trade-antibody-pb10073-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-wb-testing-1.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; Western blot analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> Lane 1: monkey COS-7 whole cell lysates, <br> Lane 2: human SH-SY5Y whole cell lysates, <br> Lane 3: rat testis tissue lysates, <br> Lane 4: mouse testis tissue lysates.<br> After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM5B antigen affinity purified polyclonal antibody (Catalog # PB10073) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM5B at approximately 180KD. The expected band size for KDM5B is at 180KD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-ihc-testing-2.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; IHC analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> KDM5B was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-ihc-testing-3.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; IHC analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> KDM5B was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-ihc-testing-4.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; IHC analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> KDM5B was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-ihc-testing-5.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; IHC analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> KDM5B was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-ihc-testing-6.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; IHC analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> KDM5B was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-ihc-testing-7.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; IHC analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> KDM5B was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-if-testing-8.jpgAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; IF analysis of KDM5B using anti-KDM5B antibody (PB10073). <br> KDM5B was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-KDM5B Antibody (PB10073) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10073-kdm5b-primary-antibodies-fcm-testing-9.pngAnti-KDM5B/PLU1/Jarid1B Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-KDM5B antibody (PB10073).<br>Overlay histogram showing U20S cells stained with PB10073 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-KDM5B Antibody (PB10073,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-krit1-picoband-trade-antibody-pb10074-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10074-1-WB-anti-krit1-picoband-antibody.jpgAnti-KRIT1 Antibody Picoband&reg; Western blot analysis of KRIT1 using anti-KRIT1 antibody (PB10074). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat cardiac muscle tissue lysates, <br> Lane 2: NIH3T3 whole cell lysates, <br> Lane 3: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRIT1 antigen affinity purified polyclonal antibody (Catalog # PB10074) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRIT1 at approximately 84 kDa. The expected band size for KRIT1 is at 84 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ldha-trade-antibody-pb10075-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-wb-testing-1.jpgAnti-LDHA Antibody Picoband&reg; Western blot analysis of LDHA using anti-LDHA antibody (PB10075). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates, <br> Lane 2: human MCF-7 whole cell lysates, <br> Lane 3: human A375 whole cell lysates,<br> Lane 4: human HepG2 whole cell lysates,<br> Lane 5: rat liver tissue lysates,<br> Lane 6: rat RH35 whole cell lysates,<br> Lane 7: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHA antigen affinity purified polyclonal antibody (Catalog # PB10075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDHA at approximately 37 kDa. The expected band size for LDHA is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-99188-g002.jpgAnti-LDHA Antibody Picoband&reg;Effects of hypoxia on SLC16A8 expression and metabolic reprogramming of colorectal cancer cells. A: The expression level of SLC16A8 in colorectal cancer cell lines; B: The expression characteristics of SLC16A8 under hypoxia; C: The effect of hypoxia on the ECAR of RKO and LoVo; D: The effect of hypoxia on the extracellular lactate levels of RKO and LoVo; E: The effect of hypoxia on the expression of PKM2 and LDHA, the key proteins of metabolic reprogramming; F: The effect of hypoxia on glucose consumption in RKO and LoVo cells. a P < 0.05, b P < 0.01, c P < 0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-99188-g005.jpgAnti-LDHA Antibody Picoband&reg;SLC16A8 siRNA reverses the effects of hypoxia on cell metabolic reprogramming. A: SLC16A8 siRNA affects the expression of SLC16A8 in hypoxia treated cells; B: Effect of SLC16A8 siRNA on ECAR of hypoxia treated cells; C: SLC16A8 siRNA can affect the level of extracellular lactic acid after hypoxia treatment; D: SLC16A8 siRNA affects the expression of PKM2 and LDHA in hypoxia treated cells; E: Effect of SLC16A8 siRNA on glucose consumption in hypoxia treated cells. a P < 0.05, b P < 0.01, c P < 0.001. 2-DG: 2-deoxyglucose.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11995316/'>40235880</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-fonc-14-1458344-g001.jpgAnti-LDHA Antibody Picoband&reg;The LDHA expression in endometrial cancer. (A) The LDHA expression summarized in the TCGA-UCEC cohort. (B) LDHA expression in paired tumor/normal EC tissues based on TCGA-UCEC cohort. (C) ROC curve analysis of LDHA. (D) The LDHA mRNA in endometrial cells detected by qRT-PCR. (E) LDHA protein stained in normal endometrial tissues by the HPA database. (F) LDHA protein stained in EC tissues by the HPA database. (G) The relationship between LDHA and clinicopathologic features. * P <0.05, ** P <0.01, *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11581964/'>39582531</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-fonc-14-1458344-g002.jpgAnti-LDHA Antibody Picoband&reg;Enrichment analysis of LDHA-related genes in EC. (A) LDHA-related genes in TCGA-UCEC cohort detected by the LinkedOmics database. The top 50 co-expression genes positively (B) and negatively (C) associated with LDHA in the TCGA-UCEC cohort. (D–F) Enrichment analysis of (GO) terms for LDHA-related genes. (G) Enrichment analysis of KEGG terms for LDHA-related genes.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11581964/'>39582531</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-fonc-14-1458344-g004.jpgAnti-LDHA Antibody Picoband&reg;Influence of LDHA knockdown on EC functions. (A) LDHA protein level after LDHA knockdown detected by Western Blot. (B, C) Cell proliferation after LDHA knockdown detected by CCK-8 assay. (D, E) Cell proliferation after LDHA knockdown detected by cell clone formation assay. (F, G) . Cell apoptosis after LDHA knockdown detected by flow cytometry. (H, I) Cell migration after LDHA knockdown was detected by the Transwell assay. (J, K) Cell invasion after LDHA knockdown was detected by the Transwell assay. * P <0.05.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11581964/'>39582531</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-fonc-14-1458344-g005.jpgAnti-LDHA Antibody Picoband&reg;Associations between LDHA and tumor immune infiltrating cells. (A) Correlation of LDHA to stromal cells and immune cells calculated by the ESTIMATE method. (B) Relationship between LDHA expression and infiltration levels of immune cells. (C) Enrichment scores of immune cells in the high LDHA group and low LDHA group. (D) Infiltration levels of immune cells in WT LDHA group and mutated LDHA group. (E) The survival curves of patients with different combinations of LDHA and immune cells. WT, wild type. * P <0.05, ** P <0.01, *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11581964/'>39582531</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-fonc-14-1458344-g006.jpgAnti-LDHA Antibody Picoband&reg;Associations between LDHA expression and ferroptosis-related genes in EC. (A) Connection of LDHA to ferroptosis-related genes in and TCGA-UCEC cohort. (B) Connection of LDHA to FANCD2 and TFRC in TCGA-UCEC cohort. (C) Connection of LDHA to FANCD2 and TFRC in . (D) The differential expression of ferroptosis-related genes between high and low LDHA groups in the TCGA-UCEC cohort. (E) Hub genes of expression association and differential expression. (F, G) The Kaplan–Meier curve of hub genes. (H, I) The changes of ferroptosis-related genes after LDHA knockdown in EC cells. * P <0.05, ** P <0.01, *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11581964/'>39582531</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-fonc-14-1458344-g007.jpgAnti-LDHA Antibody Picoband&reg;Associations between LDHA expression and m6A-related genes in EC. (A) Connection of LDHA to m6A-related genes in and TCGA-UCEC cohort. (B) Connection of LDHA to ALKBH5 and HNRNPC in TCGA-UCEC cohort. (C) Connection of LDHA to ALKBH5 and HNRNPC in . (D) The differential expression of m6A-related genes between high and low LDHA groups in the TCGA-UCEC cohort. (E) Hub genes of expression association and differential expression. (F) The Kaplan–Meier curve of hub genes. * P <0.05, *** P <0.001.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://pmc.ncbi.nlm.nih.gov/articles/PMC11581964/'>39582531</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-12935_2020_1454_fig2_html.pngAnti-LDHA Antibody Picoband&reg;The influences of circPOSTN silencing on proliferation, apoptosis and aerobic glycolysis of glioma cells. a – l LN229 and U251 cells were transfected with si-circPOSTN or si-NC. a The interference efficiency of si-circPOSTN was analyzed with RT-qPCR assay in LN229 and U251 cells. b , c Effect of circPOSTN silencing on the cell viability of LN229 and U251 cells was assessed with MTT assay. d The apoptosis rate was computed with flow cytometry assay in transfected LN229 and U251 cells. e The western blot assay showed the expression levels of Bcl-2 and Bax in LN229 and U251 cells. f The caspase-3 activity was measured with a caspase-3 assay kit. g – i The concentration of glucose and lactate in the culture medium, as well as ATP production level were measured with a series of kits, respectively. j The protein expression levels of HK2 and LDHA were determined with western blot assay in transfected LN229 and U251 cells. k – l LDHA enzyme activity and ROS accumulation were evaluated in LN229 and U251 cells post-transfection with lactate dehydrogenase activity detection kit and reactive oxygen species assay kit, respectively. * P < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-020-01454-x'>32774168</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-12935_2020_1454_fig5_html.pngAnti-LDHA Antibody Picoband&reg;CircPOSTN silencing inhibited aerobic glycolysis of glioma cells via regulating miR-361-5p. a – l LN229 and U251 cells were transfected with si-NC, si-circPOSTN, si-circPOSTN + anti-miR-NC, or si-circPOSTN + anti-miR-361-5p. a – f The concentration of glucose and lactate, as well as cellular ATP level were detected with different kits. g , h The protein expression levels of HK2 and LDHA in LN229 and U251 cells were measured with western blot assay. i – l The enzyme activity of LDHA and ROS level were measured in transfected LN229 and U251 cells. * P < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-020-01454-x'>32774168</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-12935_2020_1454_fig7_html.pngAnti-LDHA Antibody Picoband&reg;TPX2 regulated proliferation, apoptosis, and aerobic glycolysis in glioma cells. a – l LN229 and U251 cells were introduced with si-NC or si-TPX2. a The transfection efficiency of si-TPX2 was checked with RT-qPCR assay in LN229 and U251 cells. b , c The cell viability of LN229 and U251 cells was determined with MTT assay. d The apoptosis rate of transfected LN229 and U251 cells was represented by flow cytometry assay. e The western blot assay was used to assay the expression levels of Bcl-2 and Bax in LN229 and U251 cells. f The activity of caspase-3 was detected with a caspase-3 assay kit. g – i The glucose, lactate, and ATP production levels were shown. j The protein expression levels of HK2 and LDHA were estimated by western blot assay in LN229 and U251 cells. k , l LDHA enzyme activity and ROS content were evaluated in LN229 and U251 cells post-transfection. * P < 0.05 <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://link.springer.com/article/10.1186/s12935-020-01454-x'>32774168</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-wb-testing-2_1.jpgAnti-LDHA Antibody Picoband&reg; Western blot analysis of LDHA using anti-LDHA antibody (PB10075). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MSTO-211H- WT whole cell lysates, <br> Lane 2: human MSTO-211H-LDHA KO whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. Then the membrane was incubated with rabbit anti-LDHA antigen affinity purified polyclonal antibody (PB10075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for LDHA at approximately 37 kDa. The expected band size for LDHA is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-3.jpgAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-4.jpgAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-5.jpgAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-6_1_1.pngAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHAusing anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of mouse muscle skeletal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-7_1_1.pngAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of rat muscle skeletal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-8_1_1.pngAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-9_1_1.pngAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-ihc-testing-10.pngAnti-LDHA Antibody Picoband&reg; IHC analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-if-testing-11.jpgAnti-LDHA Antibody Picoband&reg; IF analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-if-testing-12_1_1.pngAnti-LDHA Antibody Picoband&reg; IF analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of mouse muscle skeletal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-if-testing-13_1_1.pngAnti-LDHA Antibody Picoband&reg; IF analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of rat muscle skeletal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-if-testing-14_2.pngAnti-LDHA Antibody Picoband&reg; IF analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-if-testing-15_1_1.pngAnti-LDHA Antibody Picoband&reg; IF analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-if-testing-16.pngAnti-LDHA Antibody Picoband&reg; IF analysis of LDHA using anti-LDHA antibody (PB10075). <br> LDHA was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 25 μg/mL rabbit anti-LDHA Antibody (PB10075) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10075-ldha-primary-antibodies-fcm-testing-17.pngAnti-LDHA Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-LDHA antibody (PB10075). <br> Overlay histogram showing A549 cells stained with PB10075 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDHA Antibody (PB10075&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ldhb-trade-antibody-pb10076-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10076-ldhb-primary-antibodies-wb-testing-1.jpgAnti-Lactate Dehydrogenase B/LDHB Antibody Picoband&reg; Western blot analysis of LDHB using anti-LDHB antibody (PB10076). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human placenta tissue lysates, <br> Lane 3: human PC-3 whole cell lysates, <br> Lane 4: monkey COS-7 whole cell lysates, <br> Lane 5: human HEK293 whole cell lysates, <br> Lane 6: human A549 whole cell lysates, <br> Lane 7: monkey kidney tissue lysates, <br> Lane 8: rat kidney tissue lysates, <br> Lane 9: rat heart tissue lysates, <br> Lane 10: mouse kidney tissue lysates, <br> Lane 11: mouse heart tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LDHB antigen affinity purified polyclonal antibody (Catalog # PB10076) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LDHB at approximately 37 kDa. The expected band size for LDHB is at 37 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10076-2-IHC-anti-ldhb-lactate-dehydrogenase-b-picoband-antibody.jpgAnti-Lactate Dehydrogenase B/LDHB Antibody Picoband&reg; IHC analysis of LDHB using anti-LDHB antibody (PB10076). <br> LDHB was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHB Antibody (PB10076) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10076-3-IHC-anti-ldhb-lactate-dehydrogenase-b-picoband-antibody.jpgAnti-Lactate Dehydrogenase B/LDHB Antibody Picoband&reg; IHC analysis of LDHB using anti-LDHB antibody (PB10076). <br> LDHB was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHB Antibody (PB10076) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10076-4-IHC-anti-ldhb-lactate-dehydrogenase-b-picoband-antibody.jpgAnti-Lactate Dehydrogenase B/LDHB Antibody Picoband&reg; IHC analysis of LDHB using anti-LDHB antibody (PB10076). <br> LDHB was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-LDHB Antibody (PB10076) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10076-5.pngAnti-Lactate Dehydrogenase B/LDHB Antibody Picoband&reg; Flow Cytometry analysis of A549 cells using anti-LDHB antibody (PB10076). <br> Overlay histogram showing A549 cells stained with PB10076 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LDHB Antibody (PB10076&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/P/B/PB10076-6.jpgAnti-Lactate Dehydrogenase B/LDHB Antibody Picoband&reg; IF analysis of LDHB using anti-LDHB antibody (PB10076).<br> LDHB was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-LDHB Antibody (PB10076) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lrig3-picoband-trade-antibody-pb10077-boster.html2026-03-24T05:05:53+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10077-1-WB-anti-lrig3-picoband-antibody.jpgAnti-LRIG3 Antibody Picoband&reg; Western blot analysis of LRIG3 using anti-LRIG3 antibody (PB10077). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates, <br> Lane 2: HEPG2 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRIG3 antigen affinity purified polyclonal antibody (Catalog # PB10077) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LRIG3 at approximately 123 kDa. The expected band size for LRIG3 is at 123 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mpp1-picoband-trade-antibody-pb10078-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10078-1-WB-anti-mpp1-picoband-antibody.jpgAnti-MPP1 Antibody Picoband&reg; Western blot analysis of MPP1 using anti-MPP1 antibody (PB10078). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat lung tissue lysates, <br> Lane 2: mouse spleen tissue lysates, <br> Lane 3: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPP1 antigen affinity purified polyclonal antibody (Catalog # PB10078) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPP1 at approximately 55 kDa. The expected band size for MPP1 is at 52 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10078-mpp1-primary-antibodies-if-testing-2.jpgAnti-MPP1 Antibody Picoband&reg; IF analysis of MPP1 using anti-MPP1 antibody (PB10078). <br> MPP1 was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MPP1 Antibody (PB10078) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mpp2-picoband-trade-antibody-pb10079-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10079-mpp2-primary-antibodies-wb-testing-1.jpgAnti-MPP2 Antibody Picoband&reg; Western blot analysis of MPP2 using anti-MPP2 antibody (PB10079). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPP2 antigen affinity purified polyclonal antibody (Catalog # PB10079) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPP2 at approximately 65 kDa. The expected band size for MPP2 is at 65 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nfia-trade-antibody-pb10080-boster.html2026-04-03T05:00:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10080-nfia-primary-antibodies-wb-testing-1.jpgAnti-NFIA Antibody Picoband&reg;Western blot analysis of NFIA using anti-NFIA antibody (PB10080). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: rat liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIA antigen affinity purified polyclonal antibody (PB10080) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NFIA at approximately 56-70 kDa. The expected band size for NFIA is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10080-nfia-primary-antibodies-ihc-testing-1.jpgAnti-NFIA Antibody Picoband&reg;IHC analysis of NFIA using anti-NFIA antibody (PB10080). <br> NFIA was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFIA Antibody (PB10080) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10080-nfia-primary-antibodies-ihc-testing-2.jpgAnti-NFIA Antibody Picoband&reg;IHC analysis of NFIA using anti-NFIA antibody (PB10080). <br> NFIA was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NFIA Antibody (PB10080) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10080-nfia-primary-antibodies-fcm-testing-1.jpgAnti-NFIA Antibody Picoband&reg;Flow Cytometry analysis of MCF-7 cells using anti-NFIA antibody (PB10080). <br> Overlay histogram showing MCF-7 cells stained with PB10080 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NFIA Antibody (PB10080, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nr2f6-picoband-trade-antibody-pb10081-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10081-1-WB-anti-nr2f6-ear2-picoband-antibody.jpgAnti-NR2F6 Antibody Picoband&reg; Western blot analysis of NR2F6 using anti-NR2F6 antibody (PB10081). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR2F6 antigen affinity purified polyclonal antibody (Catalog # PB10081) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR2F6 at approximately 43 kDa. The expected band size for NR2F6 is at 43 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pias4-picoband-trade-antibody-pb10082-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10082-1_1-WB-anti-pias4-piasy-picoband-antibody.jpgAnti-E3 SUMO-protein ligase PIAS4 Antibody Picoband&reg; Western blot analysis of PIAS4 using anti-PIAS4 antibody (PB10082). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates, <br> Lane 2: mouse testis tissue lysates, <br> Lane 3: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS4 antigen affinity purified polyclonal antibody (Catalog # PB10082) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIAS4 at approximately 57 kDa. The expected band size for PIAS4 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adfp-trade-antibody-pb10083-boster.html2026-03-27T05:07:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10083-plin2-primary-antibodies-wb-testing-1.jpgAnti-ADFP/PLIN2 Antibody Picoband&reg;Western blot analysis of ADFP/PLIN2 using anti-ADFP/PLIN2 antibody (PB10083). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human K562 whole cell lysates,<br> Lane 3: human JAR whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADFP/PLIN2 antigen affinity purified polyclonal antibody (Catalog # PB10083) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for ADFP/PLIN2 at approximately 48 kDa. The expected band size for ADFP/PLIN2 is at 48 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10083-plin2-primary-antibodies-fcm-testing-1.jpgAnti-ADFP/PLIN2 Antibody Picoband&reg;Flow Cytometry analysis of HEL cells using anti-ADFP/PLIN2 antibody (PB10083). <br> Overlay histogram showing HEL cells stained with PB10083 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ADFP/PLIN2 Antibody (PB10083, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pml-protein-trade-antibody-pb10084-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10084-pml-primary-antibodies-wb-testing-1.jpgAnti-PML Protein Antibody Picoband&reg; Western blot analysis of PML using anti-PML antibody (PB10084). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human HEK293 whole cell lysates, <br> Lane 3: human U87 whole cell lysates, <br> Lane 4: human A431 whole cell lysates, <br> Lane 5: human K562 whole cell lysates. <br> red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PML antigen affinity purified polyclonal antibody (Catalog # PB10084) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PML at approximately 90-160 kDa. The expected band size for PML is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10084-pml-primary-antibodies-ihc-testing-2.jpgAnti-PML Protein Antibody Picoband&reg; IHC analysis of PML using anti-PML antibody (PB10084). <br> PML was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PML Antibody (PB10084) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10084-3.pngAnti-PML Protein Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-PML antibody (PB10084). <br> Overlay histogram showing A431 cells stained with PB10084 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PML Antibody (PB10084&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10084-pml-primary-antibodies-ihc-testing-4.jpgAnti-PML Protein Antibody Picoband&reg; IHC analysis of PML using anti-PML antibody (PB10084). <br> PML was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PML Antibody (PB10084) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pml-protein-antibody-picoband-trade-pb10085-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10085-1-WB-anti-pml-protein-picoband-antibody.jpgAnti-PML Protein Antibody Picoband&reg; Western blot analysis of PML Protein using anti-PML Protein antibody (PB10085). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: mouse testis tissue lysates, <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PML Protein antigen affinity purified polyclonal antibody (Catalog # PB10085) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PML Protein at approximately 72 kDa, 97 kDa. The expected band size for PML Protein is at 98 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10085-2-IHC-anti-pml-protein-picoband-antibody.jpgAnti-PML Protein Antibody Picoband&reg; IHC analysis of PML Protein using anti-PML Protein antibody (PB10085). <br> PML Protein was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PML Protein Antibody (PB10085) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psat1-trade-antibody-pb10086-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10086-1-WB-anti-psat1-picoband-antibody.jpgAnti-Phosphoserine Aminotransferase/PSAT1 Antibody Picoband&reg; Western blot analysis of PSAT1 using anti-PSAT1 antibody (PB10086). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat pancreas tissue lysates, <br> Lane 2: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSAT1 antigen affinity purified polyclonal antibody (Catalog # PB10086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSAT1 at approximately 40 kDa. The expected band size for PSAT1 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10086-2-IHC-anti-psat1-picoband-antibody.jpgAnti-Phosphoserine Aminotransferase/PSAT1 Antibody Picoband&reg; IHC analysis of PSAT1 using anti-PSAT1 antibody (PB10086). <br> PSAT1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PSAT1 Antibody PB10086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10086-3-IHC-anti-psat1-picoband-antibody.jpgAnti-Phosphoserine Aminotransferase/PSAT1 Antibody Picoband&reg; IHC analysis of PSAT1 using anti-PSAT1 antibody (PB10086). <br> PSAT1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PSAT1 Antibody PB10086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10086-4-IHC-anti-psat1-picoband-antibody.jpgAnti-Phosphoserine Aminotransferase/PSAT1 Antibody Picoband&reg; IHC analysis of PSAT1 using anti-PSAT1 antibody (PB10086). <br> PSAT1 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-PSAT1 Antibody PB10086) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psma1-trade-antibody-pb10087-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10087-psma1-primary-antibodies-wb-testing-1.jpgAnti-Proteasome 20S C2/PSMA1 Antibody Picoband&reg; Western blot analysis of PSMA1 using anti-PSMA1 antibody (PB10087). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human PC-3 whole cell lysates,<br> Lane 3: human HepG2 whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 8: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA1 antigen affinity purified polyclonal antibody (Catalog # PB10087) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA1 at approximately 33 kDa. The expected band size for PSMA1 is at 30 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10087-psma1-primary-antibodies-ihc-testing-2.jpgAnti-Proteasome 20S C2/PSMA1 Antibody Picoband&reg; IHC analysis of PSMA1 using anti-PSMA1 antibody (PB10087). <br> PSMA1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (PB10087) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10087-psma1-primary-antibodies-ihc-testing-3.jpgAnti-Proteasome 20S C2/PSMA1 Antibody Picoband&reg; IHC analysis of PSMA1 using anti-PSMA1 antibody (PB10087). <br> PSMA1 was detected in a paraffin-embedded section of human rectum adenocarcinom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PSMA1 Antibody (PB10087) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psma2-picoband-trade-antibody-pb10088-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10088-1-WB-anti-psma2-picoband-antibody.jpgAnti-Proteasome 20S alpha 2/PSMA2 Antibody Picoband&reg; Western blot analysis of PSMA2 using anti-PSMA2 antibody (PB10088). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat testis tissue lysates, <br> Lane 2: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA2 antigen affinity purified polyclonal antibody (Catalog # PB10088) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA2 at approximately 26 kDa. The expected band size for PSMA2 is at 26 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psma3-trade-antibody-pb10089-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10089-psma3-primary-antibodies-wb-testing-1.jpgAnti-Proteasome 20S alpha 3/PSMA3 Antibody Picoband&reg;Western blot analysis of PSMA3 using anti-PSMA3 antibody (PB10089). <br> Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates,<br> Lane 2: human MCF-7 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human Hela whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA3 antigen affinity purified polyclonal antibody (PB10089) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PSMA3 at approximately 28 kDa. The expected band size for PSMA3 is at 28 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10089-2_1-IHC-anti-psma3-20s-proteasome-alpha-3-picoband-antibody.jpgAnti-Proteasome 20S alpha 3/PSMA3 Antibody Picoband&reg; IHC analysis of PSMA3 using anti-PSMA3 antibody (PB10089). <br> PSMA3 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PSMA3 Antibody (PB10089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10089-3_2-IHC-anti-psma3-20s-proteasome-alpha-3-picoband-antibody.jpgAnti-Proteasome 20S alpha 3/PSMA3 Antibody Picoband&reg; IHC analysis of PSMA3 using anti-PSMA3 antibody (PB10089). <br> PSMA3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PSMA3 Antibody (PB10089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10089-4_2-IHC-anti-psma3-20s-proteasome-alpha-3-picoband-antibody.jpgAnti-Proteasome 20S alpha 3/PSMA3 Antibody Picoband&reg; IHC analysis of PSMA3 using anti-PSMA3 antibody (PB10089). <br> PSMA3 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PSMA3 Antibody (PB10089) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10089-5.pngAnti-Proteasome 20S alpha 3/PSMA3 Antibody Picoband&reg; Flow Cytometry analysis of HeLa cells using anti-PSMA3 antibody (PB10089). <br> Overlay histogram showing HeLa cells stained with PB10089 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSMA3 Antibody (PB10089&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-psma4-picoband-trade-antibody-pb10090-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10090-1-WB-anti-psma4-picoband-antibody.jpgAnti-PSMA4 Antibody Picoband&reg; Western blot analysis of PSMA4 using anti-PSMA4 antibody (PB10090). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: mouse spleen tissue lysates, <br> Lane 3: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA4 antigen affinity purified polyclonal antibody (Catalog # PB10090) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA4 at approximately 29 kDa. The expected band size for PSMA4 is at 29 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-nectin-4-pvrl4-picoband-trade-antibody-pb10091-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10091-1-WB-anti-nectin-4-pvrl4-picoband-antibody.jpgAnti-PVRL4/NECTIN4 Antibody Picoband&reg; Western blot analysis of Nectin-4/PVRL4 using anti-Nectin-4/PVRL4 antibody (PB10091). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates, <br> Lane 2: MM453 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin-4/PVRL4 antigen affinity purified polyclonal antibody (Catalog # PB10091) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin-4/PVRL4 at approximately 66 kDa. The expected band size for Nectin-4/PVRL4 is at 55 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rpl19-picoband-trade-antibody-pb10092-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10092-rpl19-primary-antibodies-wb-testing-1.jpgAnti-RPL19 Antibody Picoband&reg; Western blot analysis of RPL19 using anti-RPL19 antibody (PB10092). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human MCF-7 whole cell lysates,<br> Lane 2: human A2780 whole cell lysates,<br> Lane 3: human 293T whole cell lysates,<br> Lane 4: human Jurkat whole cell lysates,<br> Lane 5: rat brain tissue lysates,<br> Lane 6: rat liver tissue lysates,<br> Lane 7: mouse brain tissue lysates,<br> Lane 6: mouse liver tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL19 antigen affinity purified polyclonal antibody (Catalog # PB10092) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL19 at approximately 26 kDa. The expected band size for RPL19 is at 23 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10092-rpl19-primary-antibodies-ihc-testing-1.jpgAnti-RPL19 Antibody Picoband&reg;IHC analysis of RPL19 using anti-RPL19 antibody (PB10092). <br> RPL19 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL19 Antibody (PB10092) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10092-2.jpgAnti-RPL19 Antibody Picoband&reg; IF analysis of RPL19 using anti-RPL19 antibody (PB10092).<br> RPL19 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RPL19 Antibody (PB10092) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10092-3.pngAnti-RPL19 Antibody Picoband&reg; Flow Cytometry analysis of U20S cells using anti-RPL19 antibody (PB10092). <br> Overlay histogram showing U20S cells stained with PB10092 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL19 Antibody (PB10092&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10092-4.pngAnti-RPL19 Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-RPL19 antibody (PB10092). <br> Overlay histogram showing A431 cells stained with PB10092 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL19 Antibody (PB10092&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mps1-trade-antibody-pb10093-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10093-rps27-primary-antibodies-wb-testing-1.jpgAnti-MPS1/RPS27 Antibody Picoband&reg; Western blot analysis of MPS1/RPS27 using anti-MPS1/RPS27 antibody (PB10093). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Caco-2 whole cell lysates,<br> Lane 2: human RT4 whole cell lysates,<br> Lane 3: human Jurkat whole cell lysates,<br> Lane 4: rat PC-12 whole cell lysates,<br> Lane 5: rat RH35 whole cell lysates,<br> Lane 6: mouse RAW264.7 whole cell lysates,<br> Lane 7: mouse SP2/0 whole cell lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPS1/RPS27 antigen affinity purified polyclonal antibody (Catalog # PB10093) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MPS1/RPS27 at approximately 13 kDa. The expected band size for MPS1/RPS27 is at 9 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10093-2-IHC-anti-mps1-picoband-antibody.jpgAnti-MPS1/RPS27 Antibody Picoband&reg; IHC analysis of MPS1 using anti-MPS1 antibody (PB10093). <br> MPS1 was detected in paraffin-embedded section of mouse brain cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MPS1 Antibody (PB10093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10093-3-IHC-anti-mps1-picoband-antibody.jpgAnti-MPS1/RPS27 Antibody Picoband&reg; IHC analysis of MPS1 using anti-MPS1 antibody (PB10093). <br> MPS1 was detected in paraffin-embedded section of rat brain cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MPS1 Antibody (PB10093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10093-4-IHC-anti-mps1-picoband-antibody.jpgAnti-MPS1/RPS27 Antibody Picoband&reg; IHC analysis of MPS1 using anti-MPS1 antibody (PB10093). <br> MPS1 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MPS1 Antibody (PB10093) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10093-5.jpgAnti-MPS1/RPS27 Antibody Picoband&reg; IF analysis of MPS1 using anti-MPS1 antibody (PB10093) <br> MPS1 was detected in paraffin-embedded section of human ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-MPS1 Antibody (PB10093) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10093-6.jpgAnti-MPS1/RPS27 Antibody Picoband&reg; IF analysis of MPS1 using anti-MPS1 antibody (PB10093).<br> MPS1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-MPS1 Antibody (PB10093) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10093-7.pngAnti-MPS1/RPS27 Antibody Picoband&reg; Flow Cytometry analysis of HeLa cells using anti-MPS1 antibody (PB10093). <br> Overlay histogram showing HeLa cells stained with PB10093 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPS1 Antibody (PB10093&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-rpsa-picoband-trade-antibody-pb10094-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10094-1-WB-anti-rpsa-picoband-antibody.jpgAnti-67kDa Laminin Receptor/RPSA Antibody Picoband&reg; Western blot analysis of RPSA using anti-RPSA antibody (PB10094). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates, <br> Lane 2: U2OS whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPSA antigen affinity purified polyclonal antibody (Catalog # PB10094) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPSA at approximately 40 kDa. The expected band size for RPSA is at 33 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10094-2.jpgAnti-67kDa Laminin Receptor/RPSA Antibody Picoband&reg; IHC analysis of RPSA using anti-RPSA antibody (PB10094). <br> RPSA was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RPSA Antibody (PB10094) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10094-3.pngAnti-67kDa Laminin Receptor/RPSA Antibody Picoband&reg; Flow Cytometry analysis of A431 cells using anti-RPSA antibody (PB10094). <br> Overlay histogram showing A431 cells stained with PB10094 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPSA Antibody (PB10094&#44;1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127&#44; 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10094-4_1_1.jpgAnti-67kDa Laminin Receptor/RPSA Antibody Picoband&reg; IF analysis of RPSA using anti-RPSA antibody (PB10094).<br> RPSA was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RPSA Antibody (PB10094) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-safb-picoband-trade-antibody-pb10095-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10095-1-WB-anti-safb-picoband-antibody.jpgAnti-SAFB Antibody Picoband&reg; Western blot analysis of SAFB using anti-SAFB antibody (PB10095). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates, <br> Lane 2: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAFB antigen affinity purified polyclonal antibody (Catalog # PB10095) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAFB at approximately 150 kDa. The expected band size for SAFB is at 103 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-sctr-picoband-trade-antibody-pb10096-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10096-1-WB-anti-sctr-secretin-r-picoband-antibody.jpgAnti-SCTR Antibody Picoband&reg; Western blot analysis of SCTR using anti-SCTR antibody (PB10096). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat kidney tissue lysates, <br> Lane 2: SKOV3 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCTR antigen affinity purified polyclonal antibody (Catalog # PB10096) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SCTR at approximately 59 kDa. The expected band size for SCTR is at 50 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-serpina1-picoband-trade-antibody-pb10097-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10097-serpina1-primary-antibodies-wb-testing-1.jpgAnti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband&reg;Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (PB10097). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human T47D whole cell lysates,<br> Lane 2: monkey COS-7 whole cell lysates,<br> Lane 3: mouse kidney tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (PB10097) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 53 kDa. The expected band size for SERPINA1 is at 47 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pc4-trade-antibody-pb10098-boster.html2026-03-24T05:05:54+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10098-sub1-primary-antibodies-wb-testing-1.jpgAnti-PC4/SUB1 Antibody Picoband&reg; Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (PB10098). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human Hela whole cell lysates, <br> Lane 2: human A549 whole cell lysates, <br> Lane 3: human MCF-7 whole cell lysates, <br> Lane 4: human T47D whole cell lysates, <br> Lane 5: human PC-3 whole cell lysates, <br> Lane 6: human Jurkat whole cell lysates, <br> Lane 7: human placenta tissue lysates, <br> Lane 8: human HL-60 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC4/SUB1 antigen affinity purified polyclonal antibody (Catalog # PB10098) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 19 kDa. The expected band size for PC4/SUB1 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10098-sub1-primary-antibodies-wb-testing-2.jpgAnti-PC4/SUB1 Antibody Picoband&reg; Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (PB10098). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat liver tissue lysates, <br> Lane 2: rat spleen tissue lysates, <br> Lane 3: rat stomach tissue lysates, <br> Lane 4: rat RH35 whole cell lysates, <br> Lane 5: mouse liver tissue lysates, <br> Lane 6: mosue spleen tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PC4/SUB1 antigen affinity purified polyclonal antibody (Catalog # PB10098) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 19 kDa. The expected band size for PC4/SUB1 is at 14 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10098-sub1-primary-antibodies-ihc-testing-3.jpgAnti-PC4/SUB1 Antibody Picoband&reg; IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (PB10098). <br> PC4/SUB1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PC4/SUB1 Antibody (PB10098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10098-sub1-primary-antibodies-ihc-testing-4.jpgAnti-PC4/SUB1 Antibody Picoband&reg; IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (PB10098). <br> PC4/SUB1 was detected in a paraffin-embedded section of human endometrioid adenocarcinoma type I tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-PC4/SUB1 Antibody (PB10098) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10098-sub1-primary-antibodies-if-testing-5.jpgAnti-PC4/SUB1 Antibody Picoband&reg; IF analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (PB10098). <br> PC4/SUB1 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PC4/SUB1 Antibody (PB10098) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-synapsin-i-trade-antibody-pb10099-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10099-2-IHC-anti-synapsin-i-picoband-antibody.jpgAnti-Synapsin I/SYN1 Antibody Picoband&reg; IHC analysis of Synapsin I using anti-Synapsin I antibody (PB10099). <br> Synapsin I was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Synapsin I Antibody (PB10099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10099-syn1-primary-antibodies-wb-testing-1.jpgAnti-Synapsin I/SYN1 Antibody Picoband&reg;Western blot analysis of Synapsin I/SYN1 using anti-Synapsin I/SYN1 antibody (PB10099). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat brain tissue lysates,<br> Lane 2: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synapsin I/SYN1 antigen affinity purified polyclonal antibody (Catalog # PB10099) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Synapsin I/SYN1 at approximately 74 kDa. The expected band size for Synapsin I/SYN1 is at 74 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-synapsin-ii-picoband-trade-antibody-pb10100-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10100-syn2-primary-antibodies-wb-testing-1_1.jpgAnti-Synapsin II/SYN2 Antibody Picoband&reg;Western blot analysis of Synapsin II using anti-Synapsin II antibody (PB10100). <br> Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human SH-SY5Y whole cell lysates,<br> Lane 2: rat brain tissue lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synapsin II antigen affinity purified polyclonal antibody (Catalog # PB10100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Synapsin II at approximately 55, 80 kDa. The expected band size for Synapsin II is at 63 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10100-syn2-primary-antibodies-ihc-testing-1.jpgAnti-Synapsin II/SYN2 Antibody Picoband&reg;IHC analysis of Synapsin II using anti-Synapsin II antibody (PB10100). <br> Synapsin II was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synapsin II Antibody (PB10100) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10100-syn2-primary-antibodies-ihc-testing-2.jpgAnti-Synapsin II/SYN2 Antibody Picoband&reg;IHC analysis of Synapsin II using anti-Synapsin II antibody (PB10100). <br> Synapsin II was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synapsin II Antibody (PB10100) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10100-syn2-primary-antibodies-ihc-testing-3.jpgAnti-Synapsin II/SYN2 Antibody Picoband&reg;IHC analysis of Synapsin II using anti-Synapsin II antibody (PB10100). <br> Synapsin II was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Synapsin II Antibody (PB10100) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10100-syn2-primary-antibodies-if-testing-1.jpgAnti-Synapsin II/SYN2 Antibody Picoband&reg;IF analysis of Synapsin II using anti-Synapsin II antibody (PB10100) and anti-Tubulin Alpha antibody (M03989-3).<br> Synapsin II was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Synapsin II Antibody (PB10100) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tgfbr1-picoband-trade-antibody-pb10101-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10101-1-WB-anti-tgfbr1-tgf-beta-receptor-i-picoband-antibody.jpgAnti-TGF beta Receptor I/TGFBR1 Antibody Picoband&reg; Western blot analysis of TGFBR1 using anti-TGFBR1 antibody (PB10101). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat cardiac muscle tissue lysates, <br> Lane 2: mouse liver tissue lysates, <br> Lane 3: HELA whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFBR1 antigen affinity purified polyclonal antibody (Catalog # PB10101) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFBR1 at approximately 56 kDa. The expected band size for TGFBR1 is at 56 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10101-41598_2025_2319_fig8_html.pngAnti-TGF beta Receptor I/TGFBR1 Antibody Picoband&reg;Impact of the knockdown of four prognostic FRGs on the migration and invasion of OS cells was investigated: The mRNA expression levels of BNIP3, G6PD, PGD, and TGFBR1 in MG-63 and SAOS-2 cells were quantified using qRT-PCR following siRNA-mediated knockdown of the four prognostic FRGs(n = 3) ( A, B ). Healing assays in MG-63 and SAOS-2 cells with siRNA knockdown of the four prognostic FRGs (100 μm) (n = 3) ( C , D ). Evaluation of invasion capabilities of MG-63 and SAOS-2 cells with siRNA knockdown of the four prognostic FRGs using Transwell assays (100 μm) (n = 3) ( E , F ). Comparison of the proportion of Edu-positive cells in MG-63 and SAOS-2 cells with siRNA knockdown of the four prognostic FRGs via Edu staining (100 μm) (n = 3) ( G ). Significance threshold was denoted as *p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41598-025-02319-x'>40399425</a></b> https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thrombopoietin-tpo-picoband-trade-antibody-pb10102-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10102-1-WB-anti-thrombopoietin-tpo-picoband-antibody.jpgAnti-Thrombopoietin/THPO Antibody Picoband&reg; Western blot analysis of Thrombopoietin/TPO using anti-Thrombopoietin/TPO antibody (PB10102). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin/TPO antigen affinity purified polyclonal antibody (Catalog # PB10102) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombopoietin/TPO at approximately 45 kDa. The expected band size for Thrombopoietin/TPO is at 38 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tiam1-picoband-trade-antibody-pb10103-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10103-1-WB-anti-tiam1-picoband-antibody.jpgAnti-TIAM1 Antibody Picoband&reg; Western blot analysis of TIAM1 using anti-TIAM1 antibody (PB10103). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: MCF-7 whole cell lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIAM1 antigen affinity purified polyclonal antibody (Catalog # PB10103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIAM1 at approximately 178 kDa. The expected band size for TIAM1 is at 178 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-tnfrsf14-hvem-picoband-trade-antibody-pb10104-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10104-1-WB-anti-tnfrsf14-hvem-picoband-antibody.jpgAnti-TNFRSF14 Antibody Picoband&reg; Western blot analysis of TNFRSF14/HVEM using anti-TNFRSF14/HVEM antibody (PB10104). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates, <br> Lane 2: SW620 whole cell lysates lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF14/HVEM antigen affinity purified polyclonal antibody (Catalog # PB10104) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF14/HVEM at approximately 37 kDa. The expected band size for TNFRSF14/HVEM is at 30 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trex2-picoband-trade-antibody-pb10105-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10105-1_1-WB-anti-trex2-picoband-antibody.jpgAnti-TREX2 Antibody Picoband&reg; Western blot analysis of TREX2 using anti-TREX2 antibody (PB10105). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: HELA whole cell lysates, <br> Lane 2: MCF-7 whole cell lysates lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREX2 antigen affinity purified polyclonal antibody (Catalog # PB10105) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREX2 at approximately 37 kDa. The expected band size for TREX2 is at 31 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-vnn1-picoband-trade-antibody-pb10106-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/b/pb10106-1_1.jpgAnti-VNN1 Antibody Picoband&reg; Western blot analysis of VNN1 using anti-VNN1 antibody (PB10106). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: SKOV3 whole cell lysates, <br> Lane 2: HELA whole cell lysates lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VNN1 antigen affinity purified polyclonal antibody (Catalog # PB10106) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VNN1 at approximately 57 kDa. The expected band size for VNN1 is at 57 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-wrn-picoband-trade-antibody-pb10107-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/pb10107-1-WB-anti-wrn-picoband-antibody.jpgAnti-Werner's syndrome helicase WRN/WRN Antibody Picoband&reg; Western blot analysis of WRN using anti-WRN antibody (PB10107). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: rat thymus tissue lysates, <br> Lane 2: human placenta tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WRN antigen affinity purified polyclonal antibody (Catalog # PB10107) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WRN at approximately 162kDa, 200 kDa. The expected band size for WRN is at 162 kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-adh5-antibody-rp1111-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1111-adh5-primary-antibodies-wb-testing-1.jpgAnti-Alcohol dehydrogenase class-3 ADH5 Antibody Picoband&reg; Western blot analysis of ADH5 using anti-ADH5 antibody (RP1111). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human HepG2 whole cell lysates, <br> Lane 2: human K562 whole cell lysates, <br> Lane 3: human MOLT4 whole cell lysates, <br> Lane 4: rat liver tissue lysates, <br> Lane 5: mouse liver tissue lysates. <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADH5 antigen affinity purified polyclonal antibody (Catalog # RP1111) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADH5 at approximately 39 kDa. The expected band size for ADH5 is at 40 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1111-2.jpgAnti-Alcohol dehydrogenase class-3 ADH5 Antibody Picoband&reg; IHC analysis of ADH5 using anti-ADH5 antibody (RP1111). <br> ADH5 was detected in paraffin-embedded section of human liver cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADH5 Antibody (RP1111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1111-3.jpgAnti-Alcohol dehydrogenase class-3 ADH5 Antibody Picoband&reg; IHC analysis of ADH5 using anti-ADH5 antibody (RP1111). <br> ADH5 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ADH5 Antibody (RP1111) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1111-4.jpgAnti-Alcohol dehydrogenase class-3 ADH5 Antibody Picoband&reg; IF analysis of ADH5 using anti-ADH5 antibody (RP1111). <br> ADH5 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-ADH5 Antibody (RP1111) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-azin2-trade-antibody-rp1112-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1112-3-IHC-anti-azin2-odcp-picoband-antibody.jpgAnti-L-Arginine decarboxylase/AZIN2 Antibody Picoband&reg;AZIN2 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody (Catalog # RP1112) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1112-2-IHC-anti-azin2-odcp-picoband-antibody.jpgAnti-L-Arginine decarboxylase/AZIN2 Antibody Picoband&reg;AZIN2 was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody (Catalog # RP1112) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/rp1112-1-WB-anti-azin2-odcp-picoband-antibody.jpgAnti-L-Arginine decarboxylase/AZIN2 Antibody Picoband&reg;Western blot analysis of AZIN2 expression in rat brain extract (lane 1)&#44; mouse brain extract (lane 2) and HELA whole cell lysates (lane 3). AZIN2 at 50KD was detected using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody (Catalog # RP1112) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/r/p/rp1112-4.jpgAnti-L-Arginine decarboxylase/AZIN2 Antibody Picoband&reg; IHC analysis of AZIN2 using anti-AZIN2 antibody (RP1112). <br> AZIN2 was detected in paraffin-embedded section of human testicar cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6&#44; epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AZIN2 Antibody (RP1112) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. https://www.bosterbio.com/picokine-elisa-kits/mouse-syndecan-1-sdc1-picokine-trade-elisa-kit-ek1554-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1554_1.pngMouse Syndecan-1/SDC1 ELISA Kit PicoKine®Mouse Syndecan-1/SDC1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-syndecan-3-sdc3-picokine-trade-elisa-kit-ek1555-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1555_1.pngHuman Syndecan-3/SDC3 ELISA Kit PicoKine®Human Syndecan-3/SDC3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-syndecan-3-sdc3-picokine-trade-elisa-kit-ek1556-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1556_1.pngMouse Syndecan-3/SDC3 ELISA Kit PicoKine®Mouse Syndecan-3/SDC3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-sap-ptx2-picokine-trade-elisa-kit-ek1557-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1557.jpgHuman SAP/PTX2/APCS ELISA Kit PicoKine®Human SAP/PTX2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-s100a8-picokine-trade-elisa-kit-ek1558-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1558_1.pngHuman S100A8/Calgranulin A ELISA Kit PicoKine®Human S100A8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-ccl25-teck-picokine-trade-elisa-kit-ek1424-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1424_1.pngMouse CCL25/TECK ELISA Kit PicoKine®Mouse CCL25/TECK PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-trkb-picokine-trade-elisa-kit-ek0848-boster.html2026-03-24T05:05:55+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0848_1.pngHuman TrkB ELISA Kit PicoKine®Human TrkB PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-fabp2-i-fabp-picokine-trade-elisa-kit-ek1410-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1410_1_2.pngHuman FABP2/I-FABP ELISA Kit PicoKine®Human FABP2/I-FABP PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-legumain-total-picokine-trade-elisa-kit-ek1566-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1566_1.pngHuman Legumain(total) ELISA Kit PicoKine®Human Legumain(total) PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-legumain-total-picokine-trade-elisa-kit-ek1567-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1567_1.pngMouse Legumain(total) ELISA Kit PicoKine®Mouse Legumain(total) PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-s100a13-picokine-trade-elisa-kit-ek1568-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1568_1.pngHuman S100A13 ELISA Kit PicoKine®Human S100A13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-s100a13-picokine-trade-elisa-kit-ek1569-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1569_1.pngMouse S100A13 ELISA Kit PicoKine®Mouse S100A13 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-dkk4-picokine-trade-elisa-kit-ek1578-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1578.jpgMouse DKK4 ELISA Kit PicoKine®Mouse DKK4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/human-chl1-l1cam-2-picokine-trade-elisa-kit-ek1576-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1576_1.pngHuman CHL1/L1CAM-2 ELISA Kit PicoKine®Human CHL1/L1CAM-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-chl1-l1cam-2-picokine-trade-elisa-kit-ek1577-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1577_1.pngMouse CHL1/L1CAM-2 ELISA Kit PicoKine®Mouse CHL1/L1CAM-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/mouse-mmp-8-picokine-trade-elisa-kit-ek1423-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1423_1.pngMouse MMP-8/Neutrophil collagenase ELISA Kit PicoKine®Mouse MMP-8 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-bdnf-picokine-trade-elisa-kit-ek0308-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0308-cn.pngDog Canine BDNF ELISA Kit PicoKine®Dog BDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-bmp-2-picokine-trade-elisa-kit-ek0313-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0313-cn.pngDog Canine BMP-2 ELISA Kit PicoKine®Dog BMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-bmp-5-picokine-trade-elisa-kit-ek0310-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0310-cn.pngDog Canine BMP-5 ELISA Kit PicoKine®Dog BMP-5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-neurotrophin-3-picokine-trade-elisa-kit-ek0473-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0473-cn.pngDog Canine Neurotrophin-3 ELISA Kit PicoKine®Dog Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-tgf-beta-1-picokine-trade-elisa-kit-ek0513-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0513-cn.pngDog Canine TGF Beta 1 ELISA Kit PicoKine®Dog TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-cadherin-2-n-cadherin-picokine-trade-elisa-kit-ek0669-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0669-cn_1.pngDog N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®Dog Cadherin-2/N-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-endothelin-1-picokine-trade-elisa-kit-ek0945-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0945-cn.jpgDog Canine Endothelin ELISA Kit PicoKine®Dog Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-il-29-picokine-trade-elisa-kit-ek0964-cn-boster.html2026-03-24T05:05:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0964-cn.pngDog Canine IL-29 ELISA Kit PicoKine®Dog IL-29 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-tgf-beta-2-picokine-trade-elisa-kit-ek0981-cn-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981-cn.pngDog Canine TGF-Beta 2 ELISA Kit PicoKine®Dog TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/dog-canine-tgf-beta-3-picokine-trade-elisa-kit-ek1103-cn-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1103-cn.pngDog Canine TGF-Beta 3 ELISA Kit PicoKine®Dog TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-activin-a-picokine-trade-elisa-kit-ek0301-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0301-bv.pngBovine Activin A ELISA Kit PicoKine®Bovine Activin A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-bdnf-picokine-trade-elisa-kit-ek0307-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0307-bv.pngBovine BDNF ELISA Kit PicoKine®Bovine BDNF 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standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-igf-1-picokine-trade-elisa-kit-ek0376-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0376-bv_1.pngBovine IGF-1 ELISA Kit PicoKine®Bovine IGF-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-neurotrophin-3-picokine-trade-elisa-kit-ek0472-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0472-bv.pngBovine Neurotrophin-3 ELISA Kit PicoKine®Bovine Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-tgf-beta-1-picokine-trade-elisa-kit-ek0513-bv-boster.html2026-03-24T05:33:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0513-bv_1.pngBovine TGF Beta 1 ELISA Kit PicoKine®Bovine TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-endothelin-picokine-trade-elisa-kit-ek0945-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0945-bv.jpgBovine Endothelin ELISA Kit PicoKine®Bovine Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-tgf-beta-2-picokine-trade-elisa-kit-ek0981-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981-bv.pngBovine TGF-Beta 2 ELISA Kit PicoKine®Bovine TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-tgf-beta-3-picokine-trade-elisa-kit-ek1103-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1103-bv.pngBovine TGF-Beta 3 ELISA Kit PicoKine®Bovine TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-cxcl14-picokine-trade-elisa-kit-ek1285-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1285-bv.jpgBovine CXCL14 ELISA Kit PicoKine®Bovine CXCL14 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/bovine-gdf5-picokine-trade-elisa-kit-ek1504-bv-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504-bv.pngBovine GDF5 ELISA Kit PicoKine®Bovine GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-activin-a-picokine-trade-elisa-kit-ek0301-po-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0301-po.pngPig porcine Activin A ELISA Kit PicoKine®Pig Activin A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-bdnf-picokine-trade-elisa-kit-ek0307-po-boster.html2026-04-04T05:00:56+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0307-po.pngPig porcine BDNF ELISA Kit PicoKine®Pig BDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-bmp-5-picokine-trade-elisa-kit-ek0310-po-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0310-po.pngPig porcine BMP-5 ELISA Kit PicoKine®Pig BMP-5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-bmp-2-picokine-trade-elisa-kit-ek0311-po-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0311-po.pngPig porcine BMP-2 ELISA Kit PicoKine®Pig BMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-tgf-alpha-picokine-trade-elisa-kit-ek0511-po-boster.html2026-03-24T05:05:57+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0511-po.pngPig porcine TGF Alpha ELISA Kit PicoKine®Pig TGF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-tgf-beta-1-picokine-trade-elisa-kit-ek0513-po-boster.html2026-04-03T05:00:49+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0513-po_1.pngPig porcine TGF Beta 1 ELISA Kit PicoKine®Pig TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-timp-3-picokine-trade-elisa-kit-ek0523-po-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0523-po.pngPig porcine TIMP-3 ELISA Kit PicoKine®Pig TIMP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-cadherin-2-n-cadherin-picokine-trade-elisa-kit-ek0669-po-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0669-po_1.pngPig N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®Pig Cadherin-2/N-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-endothelin-picokine-trade-elisa-kit-ek0945-po-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0945-po.jpgPig porcine Endothelin ELISA Kit PicoKine®Pig Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-tgf-beta-2-picokine-trade-elisa-kit-ek0981-po-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981-po.pngPig porcine TGF-Beta 2 ELISA Kit PicoKine®Pig TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-cxcl14-picokine-trade-elisa-kit-ek1285-po-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1285-po.jpgPig porcine CXCL14 ELISA Kit PicoKine®Pig CXCL14 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-epiregulin-picokine-trade-elisa-kit-ek1394-po-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1394-po.jpgPig porcine Epiregulin ELISA Kit PicoKine®Pig Epiregulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/pig-porcine-gdf5-picokine-trade-elisa-kit-ek1504-po-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504-po.pngPig porcine GDF5 ELISA Kit PicoKine®Pig GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chinese-hamster-igf-1-picokine-trade-elisa-kit-ek0377-ha-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0377-ha.pngChinese Hamster IGF-1 ELISA Kit PicoKine®Hamster IGF-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chinese-hamster-igf-2-picokine-trade-elisa-kit-ek0380-ha-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/antibody/hamster_ek0380-EK0380-HA-ELISA-chinese-hamster-igf-2-picokine-elisa-kit.pngChinese Hamster IGF-2 ELISA Kit PicoKine®Hamster IGF-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chinese-hamster-endothelin-1-picokine-trade-elisa-kit-ek0945-ha-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0945-ha.jpgChinese Hamster Endothelin ELISA Kit PicoKine®Hamster Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chinese-hamster-cxcl14-picokine-trade-elisa-kit-ek1285-ha-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1285-ha.jpgChinese Hamster CXCL14 ELISA Kit PicoKine®Hamster CXCL14 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chinese-hamster-bmp-7-picokine-trade-elisa-kit-ek1443-ha-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1443-ha.pngChinese Hamster BMP-7 ELISA Kit PicoKine®Hamster BMP-7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chinese-hamster-gdf5-picokine-trade-elisa-kit-ek1504-ha-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504-ha.pngChinese Hamster GDF5 ELISA Kit PicoKine®Hamster GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chicken-neurotrophin-3-picokine-trade-elisa-kit-ek0472-ch-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0472-ch.pngChicken Neurotrophin-3 ELISA Kit PicoKine®Chicken Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chicken-tgf-beta-2-picokine-trade-elisa-kit-ek0981-ch-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981-ch.pngChicken TGF-Beta 2 ELISA Kit PicoKine®Chicken TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chicken-tgf-beta-3-picokine-trade-elisa-kit-ek1103-ch-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1103-ch.pngChicken TGF-Beta 3 ELISA Kit PicoKine®Chicken TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/chicken-gdf5-picokine-trade-elisa-kit-ek1504-ch-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504-ch.pngChicken GDF5 ELISA Kit PicoKine®Chicken GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-bdnf-picokine-trade-elisa-kit-ek0307-eq-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0307-eq.pngHorse equine BDNF ELISA Kit PicoKine®Horse equine BDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-bmp-5-picokine-trade-elisa-kit-ek0310-eq-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0310-eq.pngHorse equine BMP-5 ELISA Kit PicoKine®Horse equine BMP-5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-bmp-2-picokine-trade-elisa-kit-ek0311-eq-boster.html2026-03-24T05:05:58+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0311-eq.pngHorse equine BMP-2 ELISA Kit PicoKine®Horse equine BMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-fgf1-picokine-trade-elisa-kit-ek0339-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0339-eq.pngHorse equine FGF1 ELISA Kit PicoKine®Horse equine FGF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-neurotrophin-3-picokine-trade-elisa-kit-ek0472-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0472-eq.pngHorse equine Neurotrophin-3 ELISA Kit PicoKine®Horse equine Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-tgf-alpha-picokine-trade-elisa-kit-ek0511-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0511-eq.pngHorse equine TGF Alpha ELISA Kit PicoKine®Horse equine TGF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-timp-3-picokine-trade-elisa-kit-ek0523-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0523-eq.pngHorse equine TIMP-3 ELISA Kit PicoKine®Horse equine TIMP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-endothelin-picokine-trade-elisa-kit-ek0945-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0945-eq.jpgHorse equine Endothelin ELISA Kit PicoKine®Horse equine Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-tgf-beta-2-picokine-trade-elisa-kit-ek0981-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981-eq.pngHorse equine TGF-Beta 2 ELISA Kit PicoKine®Horse equine TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-tgf-beta-3-picokine-trade-elisa-kit-ek1103-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1103-eq.pngHorse equine TGF-Beta 3 ELISA Kit PicoKine®Horse equine TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-cxcl14-picokine-trade-elisa-kit-ek1285-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1285-eq.jpgHorse equine CXCL14 ELISA Kit PicoKine®Horse equine CXCL14 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-gdf5-picokine-trade-elisa-kit-ek1504-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504-eq.pngHorse equine GDF5 ELISA Kit PicoKine®Horse equine GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/horse-equine-igf-1-picokine-trade-elisa-kit-ek0376-eq-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0376-eq.pngHorse equine IGF-1 ELISA Kit PicoKine®Horse equine IGF-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-activin-a-picokine-trade-elisa-kit-ek0301-rb-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0301-rb.pngRabbit Activin A ELISA Kit PicoKine®Rabbit Activin A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-bdnf-picokine-trade-elisa-kit-ek0307-rb-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0307-rb.pngRabbit BDNF ELISA Kit PicoKine®Rabbit BDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-bmp-5-picokine-trade-elisa-kit-ek0310-rb-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0310-rb.pngRabbit BMP-5 ELISA Kit PicoKine®Rabbit BMP-5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-bmp-2-picokine-trade-elisa-kit-ek0311-rb-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0311-rb.pngRabbit BMP-2 ELISA Kit PicoKine®Rabbit BMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-igf-1-picokine-trade-elisa-kit-ek0376-rb-boster.html2026-03-24T05:05:59+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0376-rb_1.pngRabbit IGF-1 ELISA Kit PicoKine®Rabbit IGF-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-neurotrophin-3-picokine-trade-elisa-kit-ek0472-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0472-rb.pngRabbit Neurotrophin-3 ELISA Kit PicoKine®Rabbit Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-tgf-alpha-picokine-trade-elisa-kit-ek0511-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0511-rb.pngRabbit TGF Alpha ELISA Kit PicoKine®Rabbit TGF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-tgf-beta-1-picokine-trade-elisa-kit-ek0513-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0513-rb.pngRabbit TGF Beta 1 ELISA Kit PicoKine®Rabbit TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-cadherin-2-n-cadherin-picokine-trade-elisa-kit-ek0669-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0669-rb.pngRabbit N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®Rabbit Cadherin-2/N-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-endothelin-1-picokine-trade-elisa-kit-ek0952-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0952-rb.jpgRabbit Endothelin 1/EDN1 ELISA Kit PicoKine®Rabbit Endothelin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-tgf-beta-2-picokine-trade-elisa-kit-ek0981-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981-rb.pngRabbit TGF-Beta 2 ELISA Kit PicoKine®Rabbit TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-tgf-beta-3-picokine-trade-elisa-kit-ek1103-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1103-rb.pngRabbit TGF-Beta 3 ELISA Kit PicoKine®Rabbit TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-sfrp5-picokine-trade-elisa-kit-ek1472-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1472-rb.pngRabbit SFRP5 ELISA Kit PicoKine®Rabbit SFRP5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-gdf5-picokine-trade-elisa-kit-ek1504-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504-rb.pngRabbit GDF5 ELISA Kit PicoKine®Rabbit GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/rabbit-grem2-picokine-trade-elisa-kit-ek1533-rb-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1533-rb.pngRabbit GREM2 ELISA Kit PicoKine®Rabbit GREM2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-activin-a-picokine-trade-elisa-kit-ek0301-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0301-pr.pngMonkey primate Activin A ELISA Kit PicoKine®Monkey Primate Activin A PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-bdnf-picokine-trade-elisa-kit-ek0307-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0307-pr.pngMonkey primate BDNF ELISA Kit PicoKine®Monkey Primate BDNF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-bmp-5-picokine-trade-elisa-kit-ek0310-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0310-pr.pngMonkey primate BMP-5 ELISA Kit PicoKine®Monkey Primate BMP-5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-bmp-2-picokine-trade-elisa-kit-ek0311-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0311-pr.pngMonkey primate BMP-2 ELISA Kit PicoKine®Monkey Primate BMP-2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-bmp-4-picokine-trade-elisa-kit-ek0314-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0314-pr.pngMonkey primate BMP-4 ELISA Kit PicoKine®Monkey Primate BMP-4 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-fgf1-picokine-trade-elisa-kit-ek0339-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0339-pr.pngMonkey primate FGF1/Fgf Acidic ELISA Kit PicoKine®Monkey Primate FGF1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-ngf-ngf-beta-picokine-trade-elisa-kit-ek0469-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0469-pr.pngMonkey primate NGF/NGF Beta ELISA Kit PicoKine®Monkey Primate NGF/NGF beta PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-neurotrophin-3-picokine-trade-elisa-kit-ek0472-pr-boster.html2026-03-24T05:06:00+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0472-pr.pngMonkey primate Neurotrophin-3 ELISA Kit PicoKine®Monkey Primate Neurotrophin-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-tgf-alpha-picokine-trade-elisa-kit-ek0511-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0511-pr.pngMonkey primate TGF Alpha ELISA Kit PicoKine®Monkey Primate TGF alpha PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-tgf-beta-1-picokine-elisa-kit-ek0513-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0513-pr.pngMonkey primate TGF Beta 1 ELISA Kit PicoKine®Monkey Primate TGF beta 1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-timp-3-picokine-trade-elisa-kit-ek0523-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0523-pr.pngMonkey primate TIMP-3 ELISA Kit PicoKine®Monkey Primate TIMP-3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-survivin-picokine-trade-elisa-kit-ek0641-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0641-pr.pngMonkey primate Survivin ELISA Kit PicoKine®Monkey Primate Survivin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-cadherin-2-n-cadherin-picokine-trade-elisa-kit-ek0669-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0669-pr_1.pngMonkey N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®Monkey Primate Cadherin-2/N-Cadherin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-cd56-ncam-1-picokine-trade-elisa-kit-ek0706-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0706-pr.pngMonkey primate CD56/NCAM-1 ELISA Kit PicoKine®Monkey Primate CD56/NCAM-1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-mif-picokine-trade-elisa-kit-ek0813-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0813-pr.pngMonkey primate MIF ELISA Kit PicoKine®Monkey Primate MIF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-fgf7-kgf-picokine-trade-elisa-kit-ek0820-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0820-pr.jpgMonkey primate FGF7/KGF ELISA Kit PicoKine®Monkey Primate FGF7/KGF PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-thbs1-tsp1-picokine-trade-elisa-kit-ek0899-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0899-pr.jpgMonkey primate THBS1/TSP1/Thrombospondin-1 ELISA Kit PicoKine®Monkey Primate THBS1/TSP1 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-thrombomodulin-picokine-trade-elisa-kit-ek0917-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0917-pr.pngMonkey primate Thrombomodulin ELISA Kit PicoKine®Monkey Primate Thrombomodulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-tnfsf13-april-picokine-trade-elisa-kit-ek0921-pr-boster.html2026-04-06T05:04:11+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0921-pr.pngMonkey primate TNFSF13/APRIL ELISA Kit PicoKine®Monkey Primate TNFSF13/APRIL PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-tgf-beta-2-picokine-trade-elisa-kit-ek0981-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0981-pr.pngMonkey primate TGF-Beta 2 ELISA Kit PicoKine®Monkey Primate TGF-beta 2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-igfbp7-picokine-trade-elisa-kit-ek0991-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek0991-pr.jpgMonkey primate IGFBP7/Igfbp Rp1 ELISA Kit PicoKine®Monkey Primate IGFBP7 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-tgf-beta-3-picokine-trade-elisa-kit-ek1103-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1103-pr.pngMonkey primate TGF-Beta 3 ELISA Kit PicoKine®Monkey Primate TGF-beta 3 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-cxcl14-picokine-trade-elisa-kit-ek1285-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1285-pr.jpgMonkey primate CXCL14/Brak ELISA Kit PicoKine®Monkey Primate CXCL14 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-epiregulin-picokine-trade-elisa-kit-ek1394-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1394-pr.jpgMonkey primate Epiregulin ELISA Kit PicoKine®Monkey Primate Epiregulin PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-sfrp5-picokine-trade-elisa-kit-ek1472-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1472-pr.pngMonkey primate SFRP5/Sarp3 ELISA Kit PicoKine®Monkey Primate SFRP5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-gdf5-picokine-trade-elisa-kit-ek1504-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1504-pr.pngMonkey primate GDF5/Bmp 14 ELISA Kit PicoKine®Monkey Primate GDF5 PicoKine ELISA Kit standard curve https://www.bosterbio.com/picokine-elisa-kits/monkey-primate-grem2-picokine-trade-elisa-kit-ek1533-pr-boster.html2026-03-24T05:06:01+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/e/k/ek1533-pr.pngMonkey primate GREM2/Prdc ELISA Kit PicoKine®Monkey Primate GREM2 PicoKine ELISA Kit standard curve https://www.bosterbio.com/products/primary-antibodies/anti-tgf-beta-1-antibody-a00019-boster.html2026-03-24T05:06:03+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-tgfb1-primary-antibodies-wb-testing-3.jpgAnti-TGF beta 1 TGFB1 AntibodyWestern Blot of Rabbit anti-TGF Beta 1 antibody. Lane 1: Reduced recombinant TGF Beta 1. Lane 2: Ladder. Lane 3: Non-reduced recombinant TGF Beta 1. Load: 250 ng per lane. Primary antibody: TGF Beta 1 antibody at 1:200 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting. Predicted/Observed size: 12 kDa/25 kDa, 12 kDa for reduced TGF Beta 1/25 kDa for non-reduced TGF Beta 1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-tgfb1-primary-antibodies-elisa-testing-2.jpgAnti-TGF beta 1 TGFB1 AntibodyELISA results of purified Rabbit anti-TGF Beta 1 Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00019-tgfb1-primary-antibodies-wb-testing-1.jpgAnti-TGF beta 1 TGFB1 AntibodyWestern blot analysis using Boster's Affinity Purified anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG (H&L) (611-132-122) diluted 1:2,500. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-h2ax-ps139-antibody-a00241-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00241-h2afx-primary-antibodies-wb-testing-2.jpgAnti-H2AX pS139 H2AFX AntibodyWestern Blot of Rabbit anti-H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 µg per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00241-h2afx-primary-antibodies-wb-testing-1.jpgAnti-H2AX pS139 H2AFX AntibodyWestern Blot of Rabbit Anti-H2AX pS139 Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: HEK293T Whole cell Lysate [+]. Lane 3: MCF-7 Whole cell Lysate [+]. Lane 4: U-87-MG Whole cell Lysate [-]. Primary Antibody: Anti-H2AXpS139 at 5µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:40,000 for 30 min at RT. Block: Universal Fluorescent Buffer for 1hr at RT. Predicted MW: ~15kDa. Observed MW: ~17kDa. Exposure: 10sec. https://www.bosterbio.com/products/primary-antibodies/anti-atr-antibody-a00262-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00262-atr-primary-antibodies-wb-testing-1.jpgAnti-ATR AntibodyWestern blot using Boster's Anti-ATR antibody. Lane 1: HeLa cell nuclear extract . Lane 2: HeLa cell nuclear extract pre-incubated with the immunizing peptide (50 µg peptide for 1 h at room temperature followed by centrifugation). Goat serum was used at 5% for blocking. Primary antibody: Anti-ATR at 1:1,400 dilution. The arrowhead corresponds to ~301kDa ATR when compared to MW markers (Lane M). https://www.bosterbio.com/products/primary-antibodies/anti-mre11-antibody-a00731-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00731-mre11-primary-antibodies-wb-testing-1.jpgAnti-Mre11 AntibodyWestern blot using Boster's Affinity Purified anti-Mre11 antibody shows detection of a band ~80 kDa corresponding to mouse Mre11 (arrowhead). Lanes 1-4 contain 0.5 ug, 0.3 ug, 0.1 ug and 0.05 ug of purified mouse Mre11 protein, respectively. After 4-20% SDS-PAGE and transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:1,000 overnight at 4°C. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-b1-antibody-a00745-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-ccnb1-primary-antibodies-wb-testing-2.jpgAnti-Cyclin B1 CCNB1 AntibodyWestern blot analysis using Boster's anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). Approximately 50 µg of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG . Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-ccnb1-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin B1 CCNB1 AntibodyBoster's anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00745-ccnb1-primary-antibodies-wb-testing-3.jpgAnti-Cyclin B1 CCNB1 AntibodyWestern blot analysis using Boster's Anti-Cyclin B1 antibody shows detection of human Cyclin B1 present in asynchronous HN30 cell lysates.  HN30 cells are from head and neck cancer tumors that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein.  The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature.  Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG 611-103-122 and chemiluminescence reagent with a 1-min exposure time.  Other detection systems will yield similar results. Personal communication, Luca Cote, Temple University, Philadelphia, PA. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mip-3-alpha-antibody-a00748-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00748-ccl20-primary-antibodyes-ihc-testing-1.jpgAnti-MIP-3 alpha CCL20 AntibodyImmunohistochemical analysis of paraffin-embedded human-pancreas&#44; antibody was diluted at 1:200.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00748-ccl20-primary-antibodyes-ihc-testing-2.jpgAnti-MIP-3 alpha CCL20 AntibodyImmunohistochemical analysis of paraffin-embedded human-liver&#44; antibody was diluted at 1:200.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00748-ccl20-primary-antibodyes-ihc-testing-3.jpgAnti-MIP-3 alpha CCL20 AntibodyImmunohistochemical analysis of paraffin-embedded human-pancreas&#44; antibody was diluted at 1:200.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00748-ccl20-primary-antibodyes-ihc-testing-4.jpgAnti-MIP-3 alpha CCL20 AntibodyImmunohistochemical analysis of paraffin-embedded human-liver&#44; antibody was diluted at 1:200. https://www.bosterbio.com/products/primary-antibodies/anti-atf3-antibody-a00904-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00904-atf3-primary-antibodies-wb-testing-2_1.jpgAnti-ATF3 AntibodyWestern blot of E.coli whole cell extract transfected with GST epitope tagged human ATF3. Boster's Affinity purified anti-ATF3 detects a band ~48 kDa corresponding to recombinant human ATF3. Immunostaining using Boster's anti-GST epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose using standard methods. After blocking with 5% goat serum and 0.5% non fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00904-atf3-primary-antibodies-wb-testing-1_1.jpgAnti-ATF3 AntibodyWestern blot of mammalian whole cell extract transfected with HA epitope tagged human ATF3. Boster's Affinity purified anti-ATF3 detects a band ~31 kDa corresponding to recombinant human ATF3. Immunostaining using Boster's anti-HA epitope tag antibody confirms the composition of the recombinant band (not shown). The protein was transferred to nitrocellulose in 30 minutes using standard methods. After blocking with 5% goat serum and 0.5% non-fat milk in PBS, the membrane was probed with the primary antibody diluted 1:200 in 0.2X blocking buffer in PBS overnight at 4°C. Reaction was followed by washes and reaction with a 1:5000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pebp2-beta-antibody-a01007-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01007-cbfb-primary-antibodies-wb-testing-2.jpgAnti-PEBP2 beta CBFB AntibodyWestern Blot analysis of various cells using PEBP2β Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01007-cbfb-primary-antibodies-wb-testing-3.jpgAnti-PEBP2 beta CBFB AntibodyWestern blot analysis of lysates from HUVEC and HeLa cells, using CBF beta Antibody. The lane on the right is blocked with the synthesized peptide.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01007-cbfb-primary-antibodies-ihc-testing-1.jpgAnti-PEBP2 beta CBFB AntibodyImmunohistochemistry analysis of paraffin-embedded human heart tissue, using CBF beta Antibody. The picture on the right is blocked with the synthesized peptide. https://www.bosterbio.com/products/primary-antibodies/anti-apolipoprotein-c-ii-antibody-a01497-boster.html2026-03-24T05:06:04+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01497-apoc2-primary-antibodies-ihc-testing-1_1.jpgAnti-Apolipoprotein C-II APOC2 AntibodyImmunohistochemistry of goat anti-Apolipoprotein C-II antibody. Tissue: human kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Apolipoprotein C-II at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Staining: Apolipoprotein C-II as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/anti-nek2-antibody-a01606-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01606-nek2-primary-antibodies-wb-testing-1_1.jpgAnti-NEK2 AntibodyWestern Blot of Rabbit Anti-NEK2 Antibody. Lane 1: Opal Pre-stained Molecular Weight Marker . Lane 2: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10µg) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10µg) . Primary Antibody: Anti-NEK2 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30mins at RT. Block: BlockOut Buffer . Expect/Observed MW: ~76kDa rec NEK2.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01606-nek2-primary-antibodies-pathway-testing-3_1.jpgAnti-NEK2 AntibodyPossible model for NEK2 action.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01606-nek2-primary-antibodies-wb-testing-2_1.jpgAnti-NEK2 AntibodyWestern blot analysis is shown using Boster's Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc. https://www.bosterbio.com/products/primary-antibodies/anti-cul5-antibody-a01925-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01925-cul5-primary-antibodies-pathway-testing-2_1.jpgAnti-Cul5/Cullin 5 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01925-cul5-primary-antibodies-ihc-testing-1_1.jpgAnti-Cul5/Cullin 5 AntibodyBoster's Anti-CUL5 antibody was diluted 1:500 to detect CUL5 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. https://www.bosterbio.com/products/primary-antibodies/anti-ldb1-antibody-a01977-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01977-ldb1-primary-antibodies-wb-testing-1_1.jpgAnti-LIM domain-binding protein 1 LDB1 AntibodyWestern blot using Boster's affinity purified anti-LDB1 antibody shows detection of LDB1 protein in Jurkat whole cell lysate (W09-001-370).  Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose.  Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.  The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).  Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-polk-antibody-a01987-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01987-polk-primary-antibodies-wb-testing-1_1.jpgAnti-POLK/Dna Polymerase Kappa AntibodyWestern blot using Boster's Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead).  Approximately 35 µg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.   After blocking the membrane was probed with the primary antibody diluted to 1:1,000.  Reaction occurred overnight at 4° followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-perk-antibody-a01992-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01992-eif2ak3-primary-antibodies-ihc-testing-2_1.jpgAnti-PERK EIF2AK3 AntibodyImmunohistochemistry staining of mouse mammary gland samples from lactating mice (L10) with Boster Immunochemical’s anti-PERK. Positive staining signal observed in wild type mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (right image) and pre-immune serum staining (left image) The anti-PERK was diluted 1:1,000 in 5% goat serum in PBS and allowed to incubate for 2h at room temperature in a humidified chamber. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01992-eif2ak3-primary-antibodies-wb-testing-1_1.jpgAnti-PERK EIF2AK3 AntibodyWestern blot analysis using Boster Immunochemical's anti-PERK to detect PERK in cell lysates. 300µg PERK over-expressing 293T cell lysate (lanes 1 & 2), or 800ug wild type (Lanes 3 & 4), and PERK knock out (lanes 5 & 6) MEF cell lysate were immunoprecipated with 15µl anti-PERK, followed by western blotting with 1:1000 dilution of anti-PERK in 5% milk/TBST buffer. Lane 1, 293T cells over-expressing Myc-PERK wt, Lane 2 , 293T cells over-expressing Myc-PERK K618A. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01992-41419_2020_2930_fig3_html.pngAnti-PERK EIF2AK3 AntibodyBCAT1 regulates autophagy through the endoplasmic reticulum stress pathway. a Expression of BCAT1 and ER-Tracker Red staining in PASMCs exposed to NOR or HYP for 24 h. Scale bar = 50 μm ( n = 3). b Western blot analysis of PERK, IRE1, ATF6, and GRP78 protein expression in the ERs pathway in PASMCs treated with gabapentin ( n = 8). c Western blot analysis of IRE1, PERK, ATF6, and GRP78 expression in PASMCs transfected with BCAT1 siRNA ( n = 8). d Western blot analysis of BECN1 and Atg5 in PASMCs treated with the ERs pathway inhibitor 4-PBA and BCAT1 plasmid ( n = 8). e Coimmunoprecipitation of the whole-cell lysates of PASMCs exposed to normoxia or hypoxia for 24 h with anti-IRE1, followed by probing with anti-BCAT1 ( n = 3). Nor normoxia, Hyp hypoxia, H + G hypoxia plus gabapentin, H + NC hypoxia plus control siRNA, H + SI hypoxia plus BCAT1 siRNA, N + Con normoxia plus control vector, H + Con hypoxia plus control vector, H + B hypoxia plus BCAT1 plasmid, H + Con+4 hypoxia plus control vector plus 4-phenylbutyric acid, H + B + 4 hypoxia plus BCAT1 plasmid plus 4-phenylbutyric acid, IP immunoprecipitation, IB immunoblotting. Statistical analysis was performed with one-way ANOVA. All values are presented as the mean ± SEM. ** p < 0.01; *** p < 0.001. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Fs41419-020-02930-y'>32938905</a></b> https://www.bosterbio.com/products/primary-antibodies/anti-taf1-antibody-a02151-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02151-taf1-primary-antibodies-wb-testing-5_1.jpgAnti-TAF1/Kat4 AntibodyWestern Blot of Rabbit anti-TAF1 antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: A549 Lysate . Lane 7: LNCap Lysate . Lane 8: MOLT-4 Lysate . Lane 9: Ramos Lysate . Lane 10: Raji Lsyate . Lane 11: A-172 Lysate . Lane 12: NIH/3T3 Lysate . Load: 35 µg per lane. Primary antibody: TAF1 antibody at 0.2ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 250kDa for TAF1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02151-taf1-primary-antibodies-chip-testing-4_1.jpgAnti-TAF1/Kat4 AntibodyTranscription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). CHIP was performed on fos promoters using 5 µg of TAF1 antibody from Boster and an RNA PolII antibody. Image with data provided courtesy of Shiraz Mujtaba, Ph.D., Dept. of Structural & Chemical Biology, Mount Sinai School of Medicine.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02151-taf1-primary-antibodies-wb-testing-2_1.jpgAnti-TAF1/Kat4 AntibodyWestern blot using Boster's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02151-taf1-primary-antibodies-ihc-testing-1_1.jpgAnti-TAF1/Kat4 AntibodyImmunohistochemistry of rabbit anti-TAF1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-TAF1 at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02151-taf1-primary-antibodies-wb-testing-3_1.jpgAnti-TAF1/Kat4 AntibodyWestern blot using Boster's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1. Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-hr23b-antibody-a02174-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02174-rad23b-primary-antibodies-pathway-testing-2_1.jpgAnti-HR23B RAD23B AntibodyNER mechanism recognizes damaged DNA regions based on their abnormal structure as well as on their abnormal chemistry, then excises and replaces them. The overall process of NER in eukaryotic cells resembles that in E. coli. The initial steps depend on whether the damage is in the actively transcribed strand of a gene or elsewhere in the genome. If the damage is not in the actively transcribed strand of a gene, then the damage is recognized and bound by a heterodimer consisting of the XPC and HR23B proteins. The binding of XPC and HR23B initiates the process of ''global genome repair'' (GGR). The XPC/HR23B dimer appears to recognize damaged DNA based on the extent of distortion of the normal helical DNA structure caused by the damage. In the process of binding to the damaged region, XPC/HR23B is thought to further increase the extent of structural distortionhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02174-rad23b-primary-antibodies-wb-testing-1_1.jpgAnti-HR23B RAD23B AntibodyWestern blot using Boster's affinity purified anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate .  Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown).   Approximately 33 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-mad2l2-antibody-a02357-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02357-mad2l2-primary-antibodies-ihc-testing-1_1.jpgAnti-MAD2L2/Mad2B AntibodyBoster's Affinity Purified anti-MAD2L2 antibody shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02357-mad2l2-primary-antibodies-wb-testing-2_1.jpgAnti-MAD2L2/Mad2B AntibodyAffinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Lane 1: HeLa whole cell lysate and nuclear lysate were probed using this antibody. This antibody clearly detects a ~20 kDa band corresponding to human MAD2L2 (predicted molecular weight is 24 kDa). Approximately 20 µg of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec. https://www.bosterbio.com/products/primary-antibodies/anti-fancc-antibody-a02387-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02387-fancc-primary-antibodies-wb-testing-1_1.jpgAnti-Fanconi anemia group C protein FANCC AntibodyWestern blot using Boster's affinity purified anti-FANCC antibody shows detection of a band at ~63 kDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate .  The identity of the lower molecular weight band is unknown.  Approximately 35µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,500 in PBS supplemented with 1% normal goat serum and 0.1% BLOTTO . The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers (indicated at left).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-artemis-antibody-a02405-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02405-dclre1c-primary-antibodies-ihc-testing-1_1.jpgAnti-Artemis DCLRE1C AntibodyBoster's Affinity Purified anti-Artemis antibody was used at a 1:1000 dilution to detect Artemis by immunohistochemistry in human spleen. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02405-dclre1c-primary-antibodies-wb-testing-2_1.jpgAnti-Artemis DCLRE1C AntibodyWestern blot using Boster's Goat-anti-Artemis was used to detect Artemis. Lane 1: AT7BI cell lysates. Lane 2: MO59K cell lysates. Lane 3: MO59J cell lysates. Lane 4: HeLa cell lysates . Lane 5: CJ cell lysates. CJ is an Artemis deficient cell line and so no band is visible. Primary Antibody at 1:500 dilution. Signal was detected using standard techniques. https://www.bosterbio.com/products/primary-antibodies/anti-gga-1-antibody-a02501-boster.html2026-03-24T05:06:05+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02501-gga1-primary-antibodies-wb-testing-1_1.jpgAnti-GGA-1 AntibodyWestern blot using Boster's Affinity Purified anti-GGA1 antibody shows detection of bands at ~100 kDa corresponding to YFP-GGA1 fusion present in a lysate of HEK293 cells over- expressing the recombinant protein (arrowhead).  Approximately 35 µg of lysate was separated on a 4-20% gel by SDS-PAGE and transferred onto nitrocellulose.   After blocking the membrane was probed with the primary antibody diluted to 1:1,350. Reaction occurred overnight at 4° followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt anti-Rb for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-myosin-6-antibody-a02627-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02627-myo6-primary-antibodies-wb-testing-1_1.jpgAnti-Myosin-6 MYO6 AntibodyWestern Blot of Rabbit anti-Myosin-6 antibody. Lane 1: SF9 cell lysate of truncated smooth myosin. Lane 2: Jurkat lysate . Lane 3: LnCap lysate . Lane 4: Recombinant myosin VI. Load: 20µg per lane for cell lysate. 50ng of recombinant protein. Primary antibody: Myosin 6 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 150 kDa for Myo6. https://www.bosterbio.com/products/primary-antibodies/anti-p29-ing4-antibody-a02664-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02664-ing4-primary-antibodies-wb-testing-1.jpgAnti-p29 ING4 AntibodyAnti-p29 ING4 polyclonal antibody detects ING4 protein by western blot.  This antibody was used at 1.0 µg/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein.  A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at ~29 kDa corresponding to ING4.   A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions.  The protein was transferred to nitrocellulose using standard methods.  After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] (code 605-432-013) for 45 min at room temperature.   LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02664-ing4-primary-antibodies-wb-testing-2.jpgAnti-p29 ING4 AntibodyAnti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates.  This antibody was used at 1.0 µg/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates.  A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells.  Personnel Communication, Motoko Unoki, NCI, NIH. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cul7-antibody-a03179-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03179-cul7-primary-antibodies-pathway-testing-2.jpgAnti-Cul7/Cullin 7 AntibodyMost modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03179-cul7-primary-antibodies-ihc-testing-1.jpgAnti-Cul7/Cullin 7 AntibodyBoster's Anti-CUL7 antibody was diluted 1:500 to detect CUL7 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. https://www.bosterbio.com/products/primary-antibodies/anti-hr23a-antibody-a03243-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03243-rad23a-primary-antibodies-wb-testing-1.jpgAnti-HR23A RAD23A AntibodyWestern blot showing Boster's Affinity Purified anti-HR23A antibody detects endogenous human HR23A. Lane 1: HeLa nuclear extract . Lane 2: HeLa . Lane 3: A431 . Lane 4: Jurkat . Lane 5: 293 whole cell lysates . Comparison to a molecular weight marker (at left) indicates a band of ~60 kDa corresponding to HR23A. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemiluminescence reagent with a 30-min exposure time. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-smarcal1-antibody-a03383-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03383-smarcal1-primary-antibodies-wb-testing-1.jpgAnti-SmarcAL1 AntibodyWestern blot using Boster's Affinity Purified anti-SmarcAL1 antibody shows detection of a band ~106 kDa band corresponding to SmarcAL1 in human derived cultured cell lysates. Lane 1: HeLa nuclear extract , Lane 2: HeLa WC , Lane 3: A431 WC , Lane 4: Jurkat WC , and 293 WC . Approximately 5µg of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SmarcAL1 antibody. Signal was detected using standard techniques. SmarcAL1 is the band seen between the 97 and 116kD markers. https://www.bosterbio.com/products/primary-antibodies/anti-fanca-antibody-a03662-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03662-fanca-primary-antibodies-wb-testing-2.jpgAnti-FANCA/Faca AntibodyWestern blot using Boster's affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corresponding to FANCA in HeLa whole cell lysates .  The identity of the lower molecular weight bands is unknown but may represent breakdown products.  Approximately 35µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers  indicated at left (700 nm channel, red).  IRDye™800 fluorescence images were captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03662-fanca-primary-antibodies-wb-testing-1.jpgAnti-FANCA/Faca AntibodyWestern blot using Boster's affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates.  No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301. https://www.bosterbio.com/products/primary-antibodies/anti-cbl-c-antibody-a03693-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03693-cblc-primary-antibodies-wb-testing-1.jpgAnti-Cbl-c AntibodyImmunoprecipitation (right) and western blot (left) using Boster's Affinity Purified anti-Cbl-c antibody shows detection of a predominant band at ~52 kDa corresponding to Cbl-c. Lysates used are from HEK293T cells transfected with empty vector or with Cbl-c and western blotting (left panel). The predicted size of Cbl-c is 52 kDa. Size markers in kDa are shown to the left of the panel. The (right panel) shows immunoprecipitation with Rabbit anti-Cbl-c followed by western blotting using a Goat anti-Cbl-c antibody. Personal Communication. Stan Lipkowitz, NCI, NIH, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03693-cblc-primary-antibodies-ihc-testing-3.jpgAnti-Cbl-c AntibodyBoster's affinity purified anti-Cbl-c antibody was used at 5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining of human pancreatic acinar epithelium at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03693-cblc-primary-antibodies-ihc-testing-4.jpgAnti-Cbl-c AntibodyBoster's Affinity Purified anti-Cbl-c antibody shows strong nuclear and cytoplasmic staining of cells in tubuli in human kidney tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden. https://www.bosterbio.com/products/primary-antibodies/anti-rreb1-antibody-a03902-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03902-rreb1-primary-antibodies-ihc-testing-1.jpgAnti-RREB1 AntibodyImmunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RREB1 Antibody. The picture on the right is blocked with the synthesized peptide. https://www.bosterbio.com/products/primary-antibodies/anti-bach2-antibody-a04096-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04096-bach2-primary-antibodies-wb-testing-1.jpgAnti-Bach2 AntibodyWestern Blot of Rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 µg per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted (native)/Observed (over-expressed) size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thymidylate-synthase-antibody-a04320-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-tyms-primary-antibodies-wb-testing-3.jpgAnti-Thymidylate Synthase TYMS AntibodyAnti-Thymidylate Synthase is shown to detect thymidylate synthase present in a HeLa cell lysate . Each lane is estimated to contain 4µg of protein. Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-tyms-primary-antibodies-ihc-testing-1.jpgAnti-Thymidylate Synthase TYMS AntibodyImmunohistochemistry of Thymidylate Synthase antibody. Tissue: human Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: 100-601-199 Thymidylate antibody at 1:100. Secondary antibody: Peroxidase goat anti-sheep at 1:10,000 for 45 min at RT. Image provided courtesy of Andrew Elston, LifeSpan BioSciences, Inc. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thymidylate-synthase-antibody-a04320-1-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04320-1-tyms-primary-antibodies-wb-testing-1.jpgAnti-Thymidylate Synthase TYMS AntibodyAnti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:500, 1:1,000 and 1:2,000 fold dilutions of the antibody. Detection was made using HRP Goat-a-Rabbit IgG (611-1302) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'. https://www.bosterbio.com/products/primary-antibodies/anti-sprouty-4-antibody-a04343-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-spry4-primary-antibodies-ihc-testing-1.jpgAnti-Sprouty-4 SPRY4 AntibodyImmunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-spry4-primary-antibodies-ihc-testing-3.jpgAnti-Sprouty-4 SPRY4 AntibodyImmunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Sprouty-4 antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Sprouty-4 is cytoplasmic. Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04343-spry4-primary-antibodies-wb-testing-4.jpgAnti-Sprouty-4 SPRY4 AntibodyWestern Blot of Rabbit anti-Sprouty-4 antibody. Lane 1: HeLa . Lane 2: SW13. Lane 3: C2C12 . Load: 30 µg per lane. Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: a doublet band ~35 kDa corresponding to human Sprouty-4. Other band(s): none. https://www.bosterbio.com/products/primary-antibodies/anti-boris-antibody-a04503-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04503-ctcfl-primary-antibodies-wb-testing-1.jpgAnti-BORIS CTCFL AntibodyWestern blot using Boster's Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead).   Lane 1 contains lysate from human prostate tissue.  Lane 2 contains lysate from human spleen tissue.  A predominant band at ~75 kDa is observed.  Molecular weight estimation was made by comparison to prestained MW markers as indicated. https://www.bosterbio.com/products/primary-antibodies/anti-cyclin-e2-antibody-a04536-boster.html2026-03-24T05:06:06+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04536-ccne2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin E2 CCNE2 AntibodyWestern blot using Boster's affinity purified anti-Cyclin E2 antibody shows specific detection of Cyclin E2. Cell extracts over-expressing mouse Cyclin E1 (lane 1) and Cyclin E2 (lane 2) were electrophoresed, transferred to nitrocellulose, and probed with the anti-Cyclin E2 antibody. The affinity purified antibody also detects endogenous Cyclin E2 in Skp2-/- MEF cells. (data not shown). Personal Communication, Philipp Kaldis, CCR-NCI, Frederick, MD. https://www.bosterbio.com/products/primary-antibodies/anti-slit-3-antibody-a04745-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04745-slit3-primary-antibodies-wb-testing-1.jpgAnti-SLIT-3 AntibodyWestern blot using Boster's Affinity Purified anti-SLIT-3 antibody shows detection of a predominant band at ~145 kDa corresponding to SLIT-3 (arrowhead) in a bovine thyroid whole cell lysate using the 800 nm channel (green). ~ 35 ug of lysate was separated on a 4-8% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:800. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu (611-432-122) for 45 min at room temperature. Molecular weight markers were used for size comparison using the 700 nm channel (not shown). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-p28-ing5-antibody-a04974-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04974-ing5-primary-antibodies-wb-testing-1.jpgAnti-p28 ING5 AntibodyWestern blot analysis is shown using Boster's Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time. Other detection systems will yield similar results. Personal communication Jacques Cote.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04974-ing5-primary-antibodies-wb-testing-2.jpgAnti-p28 ING5 AntibodyWestern blot analysis is shown using Boster's Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4° C followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-p47-ing3-antibody-a05458-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05458-ing3-primary-antibodies-diagram-testing-2.jpgAnti-p47 ING3 AntibodyPanel A shows the four isoforms of ING1 generated by alternative splicing. Panel B shows four additional ING proteins 2-5 that also contain similar motifs, namely, PHD domains, NLS motifs, and, for ING2, a leucine zipper domain which promotes protein interactions through hydrophobic interactions.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05458-ing3-primary-antibodies-wb-testing-1.jpgAnti-p47 ING3 AntibodyWestern blot using Boster's purified anti-ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 µg of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4° C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-kmo-antibody-a05469-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05469-kmo-primary-antibodies-wb-testing-1.jpgAnti-Kynurenine 3-monooxygenase KMO AntibodyWestern Blot of Rabbit anti-KMO antibody. Lane 1: Brain Extract. Load: 10 µg per lane. Primary antibody: KMO antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 58 kDa for KMO. Other band(s): None. https://www.bosterbio.com/products/primary-antibodies/anti-ahsp-antibody-a05948-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-ihc-testing-1.jpgAnti-AHSP/Eraf AntibodyImmunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A.) Normal bone marrow, H&E. B.) CD235a stains both nucleated EPs and mature, anucleate RBCs. C.) AHSP stains nucleated EPs, but not mature, anucleate RBCs. D.) CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C. Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ – fully automated for IHC.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-ihc-testing-2.jpgAnti-AHSP/Eraf AntibodyImmunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A.) Acute erythroleukemia, H&E. B.) CD235a stains erythroid blasts and mature, anucleate RBCs. C.) AHSP stains erythroid blasts. D.) CD71 stains erythroid blasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C. Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ – fully automated for IHC.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-ihc-testing-3.jpgAnti-AHSP/Eraf AntibodyImmunohistochemistry of Rabbit anti-AHSP antibody. CD71 stains myeloid blasts in acute myeloid leukemia, whereas AHSP does not. AHSP stains residual EPs and not myeloid blasts in acute myeloid leukemia with minimal differentiation. (A), whereas CD71 stains both myeloid blasts and EPs (B). AHSP does not stain myeloid blasts in acute myelomonocytic leukemia (D), whereas CD71 does (E). C and F are corresponding H&Es, respectively. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ – fully automated for IHC.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-ihc-testing-4.jpgAnti-AHSP/Eraf AntibodyImmunohistochemistry of Rabbit anti-AHSP antibody. AHSP and CD71 stain megakaryocytes in primary myelofibrosis. Tissue: A.) Primary myelofibrosis, H&E. B.) CD235a stains both nucleated EPs and mature, anucleate RBCs. AHSP C.) and CD71. D.) variably stain megakaryocytes and also stain nucleated EPs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ – fully automated for IHC.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-ihc-testing-5.jpgAnti-AHSP/Eraf AntibodyImmunohistochemistry of Rabbit anti-AHSP antibody. AHSP (A) stains residual EPs and not lymphoma cells in DLBCL, whereas CD71 (B) stains both lymphoma cells and EPs. C. Corresponding H&E. AHSP (D) does not metastatic carcinoma, whereas CD71 (E) does. F. Corresponding H&E. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ – fully automated for IHC.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-ihc-testing-6.jpgAnti-AHSP/Eraf AntibodyImmunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A.) Giant pronormoblasts are evident in parvoviral infection, H&E. B.) CD235a does not stain giant pronormoblasts. C.) AHSP and D.) CD71 stain giant pronormoblasts. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin. Antigen retrieval: TRIS-EDTA pH9.0. Primary antibody: AHSP antibody at 1:8,000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at 1:10,000 for 45 min at RT. Localization: Anti-AHSP is cytoplasmic. Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max™ – fully automated for IHC.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-wb-testing-8.jpgAnti-AHSP/Eraf AntibodyWestern Blot of Rabbit anti-AHSP antibody. Lane 1: Recombinant hAHSP (10ng). Lane 2: Recombinant mAHSP (10ng). Lane 3: mice Spleen cells transfected with TNS9.2-hAHSP. Lane 4: mice Spleen cells transfected with TNS9.2 control vector. Lane 5: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by MACS using Ter119+ microbeads. Lane 6: mice Spleen cells transfected with TNS9.2 control vector fractionated by Ter119+. Lane 7: mice Spleen cells transfected with TNS9.2-hAHSP fractionated by Ter119-. Lane 8: Spleen cells from mice transduced with TNS9.2 control vector fractionated by Ter119-. Load: 10 ng per lane. Primary antibody: AHSP antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 5% dry milk 30 min at RT. Predicted/Observed size: ~12kDa. Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05948-ahsp-primary-antibodies-wb-testing-9.jpgAnti-AHSP/Eraf AntibodyWestern Blot of Rabbit anti-AHSP antibody. Lane 1: Recombinant hAHSP (2 ng). Lane 2: Recombinant mAHSP (2 ng). Lane 3: RBC Lysates Mouse #24 - GFP. Lane 4: RBC Lysates Mouse #25 - GFP. Lane 5: RBC Lysates Mouse #16 - hAHSP. Lane 6: RBC Lysates Mouse #17 - hAHSP. Lane 7: RBC Lysates Mouse #22 - hAHSP. Lane 8: RBC Lysates Mouse #19 - hAHSP. Lane 9: RBC Lysates Mouse #20 - hAHSP. Load: if not described differently, 10 ng per lane. Primary antibody: hAHSP antibody, Beta-Actin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 5% dry milk 30 min at RT. Predicted/Observed size: ~12kDa. Other band(s): none. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-t2-antibody-a06015-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06015-ccnt2-primary-antibodies-wb-testing-1.jpgAnti-Cyclin T2 CCNT2 AntibodyWestern blot using Boster's Anti-Cyclin T2 (Rabbit) is shown to detect two major bands (arrowheads) corresponding to human Cyclin T2a (~74kDa) and T2b (~81kDa) as indicated. Approximately 33 µg of a HeLa whole cell lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane with 5% BLOTTO in PBS, the membrane was probed for overnight at 4° C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-morc3-antibody-a06075-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06075-morc3-primary-antibodies-wb-testing-1.jpgAnti-Morc3 AntibodyWestern Blot of Rabbit anti-Morc3 antibody. Lane 1: Human embryonic stem cell. Lane 2: Human embryonic stem cell. Lane 3: C-Flag Mouse embryonic stem cell. Lane 4: C-Flag Mouse embryonic stem cell doxycycline induced. Load: 35 µg per lane. Primary antibody: hMorc3 antibody at 1:1000-1:5000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 107kDa/ ~170kDa. Other band(s): sumoylated Morc run higher. https://www.bosterbio.com/products/primary-antibodies/anti-casz1-antibody-a06443-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06443-casz1-primary-antibodies-ihc-testing-3.jpgAnti-CASZ1 AntibodyImmunohistochemistry results of Rabbit Anti-hCASZ1 Antibody. Tissue: NB patient tumor. A. Score 0- a rare positive nuclei. B. Score 1- (1-10% positive) equivocal/uninterpretable. C. Score 2- (10-50% positive) weak positive. D. Score 3- (>50% positive) strong positive. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue). Localization: Nuclear.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06443-casz1-primary-antibodies-ihc-testing-4.jpgAnti-CASZ1 AntibodyImmunohistochemistry results of Rabbit Anti-hCasz1 Antibody. Tissue: NB patient tumor. A. CASZ1 localized exclusively in the cytoplasm. B. CASZ1 localized in the cytoplasm and nucleus. Primary Antibody: Rabbit Anti-CASZ1 stained brown. Nucleus counterstained with hematoxylin (blue).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06443-casz1-primary-antibodies-if-testing-5.jpgAnti-CASZ1 AntibodyImmunofluorescence results of Endogenous CASZ1. Cells: BE2 cells. With or without Pre-Incubation of Anti-CASZ1 Antibody with CASZ1 Peptide. Staining: Rabbit Anti-CASZ1 Antibody. Chromatin counter stain: DAPI.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06443-casz1-primary-antibodies-if-testing-6.jpgAnti-CASZ1 AntibodyImmunofluorescence results of Rabbit Anti-CASZ1 Antibody. Tissue: Mouse Xenograft tumor of human NB cell line transfected with or without tetracycline inducible CASZ1 (NGPtetCASZ1a). Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06443-casz1-primary-antibodies-if-testing-7.jpgAnti-CASZ1 AntibodyImmunofluorescence of Rabbit anti-CASZ1 Antibody. Tissue: adult murine ocular tissue. Antibody: Rabbit Anti-CASZ1 Antibody. Counterstain: DAPI. Localization: nucleus in lens epithelia but primarily localizes in the cytoplasm in photoreceptor cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06443-casz1-primary-antibodies-wb-testing-1.jpgAnti-CASZ1 AntibodyWestern blot using Boster's anti-hCASZ1 antibody. This blot shows detection of endogenous and transfected human CASZ1 protein in fresh whole cell lysate (~30 µg). Lane 1: BE2(s) cell lysate. Lane 2: BE2(N) cell lysate. Lane 3: SY5Y transfected with hCASZ5 (125kDa). Lane 4: SY5Y transfected with hCASZ11 (190kDa). Protein was resolved by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000 for 1.5 hours at room temperature then incubated with HRP-conjugated Goat Anti-Rabbit antibody for 45 min. at room temperature. Personal communication, Carol Thiele, NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06443-casz1-primary-antibodies-wb-testing-2.jpgAnti-CASZ1 AntibodyWestern Blot of Anti-CASZ1 Antibody. Lane 1: NBLS Cytoplasmic (20µg). Lane 2: NBLS Nuclear (3µg). Lane 3: BE2C Cytoplasmic (30µg). Lane 4: BE2C Nuclear (7µg). Lane 5: SY5Y-CASZ1b (10µg). Block: 5% Blotto/TTBS for 1 hour. Primary: Casz1 1:10,000 for 1 hour. Secondary: Goat anti-Rabbit HRP for 1 hour. 240sec exposure. Detects nuclear endogenous CASZ1a and CASZ1b; and transiently transfected CASZ1b isoform. Personal communication and images from Carol Thiele Galetto, NCI. https://www.bosterbio.com/products/primary-antibodies/anti-neuraminidase-antibody-a06481-boster.html2026-03-24T05:06:07+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06481-neu2-primary-antibodies-wb-testing-1.jpgAnti-Neuraminidase NEU2 AntibodyWestern blot analysis using Boster Immunochemical's Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 µg loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kDa). The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:1,000. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06481-neu2-primary-antibodies-wb-testing-2.jpgAnti-Neuraminidase NEU2 AntibodyWestern blot analysis using Boster Immunochemical's Affinity Purified anti-Neu2 antibody to detect Neu-2 present in a lysate expressing human Neu2 (1.0 ul loaded). Molecular weight marker (not shown) indicates a band of the expected MW (43 kDa). The reactive lower molecular weight band is believed to represent a truncated form of this protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] (611-132-122) diluted 1:1,000. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-nf-y-antibody-a06714-boster.html2026-03-24T05:06:08+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/anti-ube2j1-antibody-a07059-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07059-ube2j1-primary-antibodies-pathway-testing-2.jpgAnti-UBE2J1/Ubc6E AntibodyMost modifiers mature by proteolytic processing from inactive precursors (“a” = amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thioesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein)/CBC(cullin-2/elonginB/elonginC)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. (From Jentsch & Pyrowolakis (2000); see references below.)https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07059-ube2j1-primary-antibodies-wb-testing-1.jpgAnti-UBE2J1/Ubc6E AntibodyWestern blot using Boster's affinity purified anti-Ube2j1 antibody shows detection of Ube2j1 in 293 cells over-expressing Myc-Ube2j1 (Lane 2). Lane 1 contains lysate from mock-transfected 293 cells. Personal Communication, A. Weissman & T. Shang, CCR-NCI, Frederick, MD https://www.bosterbio.com/products/primary-antibodies/anti-ap3d1-antibody-a07250-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07250-ap3d1-primary-antibodies-wb-testing-1.jpgAnti-AP3D1/Ap3 AntibodyWestern blot using Boster's Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate . The lower molecular weight band most likely represents non-specific binding. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 30-sec exposure time. https://www.bosterbio.com/products/primary-antibodies/anti-aspp1-antibody-a07257-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07257-ppp1r13b-primary-antibodies-wb-testing-1.jpgAnti-ASPP1 Ppp1r13b AntibodyWestern blot using Boster's affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK. https://www.bosterbio.com/products/primary-antibodies/anti-magp-2-antibody-a07400-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07400-mfap5-primary-antibodies-wb-testing-1.jpgAnti-MAGP-2 MFAP5 AntibodyWestern blot using Boster's affinity purified anti-MAGP-2 antibody shows detection (arrowhead) of secreted MAGP-2 (lane 2) and MAGP-2 present in a MAGP-2 transfected HEK293 lysate (lane 3). No staining is detected in supernatants from non-transfected cells (lane 1). After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:100 in TBST with 5% BSA overnight at 4° C. Personal Communication, Michael Birrer, CCR-NCI, Bethesda, MD https://www.bosterbio.com/products/primary-antibodies/anti-pacrg-antibody-a07487-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07487-pacrg-primary-antibodies-wb-testing-1.jpgAnti-PACRG AntibodyWestern blot using Boster's Affinity Purified anti-PACRG antibody shows detection of a band ~40 kDa corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-myosin-1g-antibody-a08448-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08448-myo1g-primary-antibodies-wb-testing-1.jpgAnti-Myosin 1G MYO1G AntibodyWestern blot using Boster's affinity purified anti-Myosin 1G antibody. Lane 1: Jurkat cell lysates [+]. Lane 2: peripheral blood T cell lysates [+]. Lane 3: human spleen lysates [+]. Lane 4: 300.19 cell lysates [+]. Lane 5: 293 cell lysates [-]. Shows detection of a band ~100kDa in size corresponding to Myosin 1G (arrowhead) in Myosin 1G positive whole cell lysates. Personal Communication. Stephen Shaw, NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-ankrd26-antibody-a09289-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09289-ankrd26-primary-antibodies-wb-testing-1.jpgAnti-Ankrd26 AntibodyWestern blot using Boster's affinity purified anti-Ankrd26 antibody shows detection of a band at ~81 kDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 µg, Lane 3 MES cell lysate - 40 µg, Lane 4 293T-ANKRD26 transfected cell lysate - 20 µg, Lane 5 control 293T cell lysate - 20 µg, Lane 6 BSA-ANKRD26 conjugate 20 ng, Lane 7 BSA - 500 ng, Lane 8 BSA - 100 ng, Lane 9 BSA 20 ng and Lane 10 Protein standards. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG (611-105-122 is suggested) diluted to 1:3,000. Size estimation was made by comparison to prestained MW markers as indicated. Personal Communication. Ira Pastan, NIH, CCR, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-pip5k2b-antibody-a09390-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09390-pip4k2b-primary-antibodies-wb-testing-1.jpgAnti-PIP5K2B PIP4K2B AntibodyWestern Blot of Rabbit Anti-PIP5K2B Antibody. Lane 1: Mouse Brain homogenate samples. Load: 10 µg per lane. Primary antibody: PIP5K2B antibody at 0.2µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None. https://www.bosterbio.com/products/primary-antibodies/anti-sts-1-antibody-a09570-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09570-ubash3b-primary-antibodies-ihc-testing-1.jpgAnti-Sts-1 UBASH3B AntibodyImmunohistochemistry with anti-Sts-1 antibody showing Sts-1 staining of histiocytic elements in cytoplasm of human tonsil at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-Sts-1 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3’3-diamino-benzidine substrate (DAB).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a09570-ubash3b-primary-antibodies-wb-testing-2.jpgAnti-Sts-1 UBASH3B AntibodyWestern blot using Boster's Affinity Purified anti-Sts-1 antibody shows detection of a band ~70 kDa corresponding to mouse Sts-1. Approximately 1.0 µg of recombinant (truncated) Sts-1-GST was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 0.3ug/ml overnight at 4° C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-tmbim1-antibody-a10501-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10501-tmbim1-primary-antibodies-wb-testing-4.jpgAnti-TMBIM1/Recs1 AntibodyWestern blot using Boster's protein A purified anti-TMBIM1 antibody shows detection of exogenous TMBIM1 in lysates from HeLa cells transfected with pcDNA3-hTMBIM1 (lane 1). No staining is observed in lysates from mock transformed HeLa cells (lane 2). To date this antibody has shown the ability to recognize over expressed TMBIM1 ~35kDa, but not endogenous protein. The membrane was probed with the primary antibody at a 1:1,000 dilution at 4° C, overnight. Personal Communication from Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10501-tmbim1-primary-antibodies-ihc-testing-3.jpgAnti-TMBIM1/Recs1 AntibodyImmunohistochemistry of Rabbit anti-TMBIM1 antibody. Tissue: human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMBIM1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TMBIM1 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10501-tmbim1-primary-antibodies-ihc-testing-1.jpgAnti-TMBIM1/Recs1 AntibodyImmunohistochemistry of Rabbit anti-TMBIM1 antibody. Tissue: human prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TMBIM1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TMBIM1 as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/anti-rnf25-antibody-a10595-boster.html2026-03-24T05:06:08+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10595-rnf25-primary-antibodies-wb-testing-1.jpgAnti-RNF25 AntibodyWestern blot using Boster's affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10595-rnf25-primary-antibodies-ip-testing-2.jpgAnti-RNF25 Antibody1 µg of Boster’s affinity purified anti-RNF25 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-tamalin-antibody-a11078-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11078-grasp-primary-antibodies-wb-testing-1.jpgAnti-Tamalin GRASP AntibodyWestern blot using Boster's affinity purified anti-Tamalin to detect over-expressed Tamalin in HEK293 cells (lane 2, arrowhead). Lane 1 shows the non-transfected control. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:2,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11078-grasp-primary-antibodies-wb-testing-3.jpgAnti-Tamalin GRASP AntibodyMouse brain lysate was immunoprecipitated with anti-Tamalin antiserum. A blot was prepared and probed with affinity purified anti-Tamalin. Lane 1 is wild-type brain lysate; Lane 2 is Tamalin knock-out brain lysate. The membrane was probed with the primary antibody at a 1:1,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD. https://www.bosterbio.com/products/primary-antibodies/anti-cenp-q-antibody-a11703-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11703-cenpq-primary-antibodies-if-testing-1.jpgAnti-CENP-Q AntibodyImmunofluorescence microscopy using Boster's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in HeLa whole cell lysate. Primary antibody was used at 1:100 followed by secondary antibody diluted 1:150. Red punctate anti-CENP-Q signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENP-Q and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MDhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a11703-cenpq-primary-antibodies-wb-testing-2.jpgAnti-CENP-Q AntibodyWestern blot using Boster's protein A purified anti-CENP-Q antibody shows detection of endogenous CENP-Q in a HeLa whole cell lysate (lane 1, arrowhead). The blot was incubated for 1.5 hours at room temperature using the primary antibody diluted to 0.5µg/mL, followed by washes and incubation with to the secondary antibody. Lane 1: Lysates from HeLa cells transfected with control sh-virus wherein the expression of CENP-Q is expected to not to alter. Lane 2: Lysates from HeLa cells transfected with Cenp-Q sh-virus wherein the expression of CENP-Q is knocked down significantly to a level where it is not being detected at all under the tested condition/WB exposure time. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-ap1g1-antibody-a12998-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12998-ap1g1-primary-antibodies-wb-testing-1.jpgAnti-AP-1 complex subunit gamma-1 AP1G1 AntibodyWestern blot using Boster's Affinity Purified anti-AP1G1 antibody shows strong detection of a 91-kDa band corresponding to Human AP1G1. Lane 1: HeLa whole cell lysate . Lane 2: Peptide competition (using 1 µg/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time. https://www.bosterbio.com/products/primary-antibodies/anti-hus1b-antibody-a13393-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a13393-hus1b-primary-antibodies-wb-testing-1.jpgAnti-Checkpoint protein HUS1B Hus1B AntibodyWestern blot using Boster's affinity purified anti-Hus1B antibody shows detection of a 36kDa band [arrow] corresponding to Hus1B in a HeLa cell lysate . The staining pattern in 293 cells is less clear, showing a predominant band at 39 kDa. Personal Communication, A-Lien Lu-Chang, U. Maryland, Baltimore, MD. https://www.bosterbio.com/products/primary-antibodies/anti-histone-h4-antibody-a14495-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14495-hist1h4a-primary-antibodies-wb-testing-1.jpgAnti-Histone H4 HIST1H4A AntibodyWestern Blot of Rabbit anti-Histone H4 antibody. Lane 1: HeLa Whole Cell Lysate . Lane 2: HeLa Nuclear Extract . Lane 3: HeLa Whole Cell Lysate . Lane 4: HeLa Nuclear Extract . Load: 15 µg per lane. Primary antibody: Lanes 1-2: Histone H3 K18Ac, Lanes 3-4: Histone H4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: overnight at 4°C. Predicted/Observed size: ~15.4kDa for Histone H3, and 11.3kDa for Histone H4.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14495-hist1h4a-primary-antibodies-dot-blot-testing-3.jpgAnti-Histone H4 HIST1H4A AntibodyDot Blot of Rabbit Histone H4 Antibody. Lane 1: AC. Lane 2: NM. Load: 100, 10, 1 picomoles of peptide. Primary antibody: Histone H4 antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Gt-anti-Rb IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. https://www.bosterbio.com/products/primary-antibodies/anti-spanx-c-antibody-a14601-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a14601-spanxc-primary-antibodies-wb-testing-1.jpgAnti-SPANX-C AntibodyWestern blot using Boster's affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells in Lane 1. VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein ~97kDa. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers. Personal Communication. Vladimir Larionov, NIH, CCR, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-bivm-antibody-a15680-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a15680-bivm-primary-antibodies-wb-testing-1.jpgAnti-BIVM AntibodyWestern blot of anti-human BIVM. Whole cell HeLa lysate was used to probe for endogenous BIVM using Rabbit-anti-Human BIVM (C-terminal specific) polyclonal antibody. A 57 kDa band corresponding to human BIVM protein is detected using a 1:1,000 dilution of the antiserum incubated for 1 hour at room temperature. Washes with 1% Tween-20 TBS preceded reaction with HRP Gt-a-Rabbit IgG (H&L) code 611-103-122 diluted 1:10,000 and incubated for 1 hour at room temperature. The blot was developed using a chemiluminescent detection method (AP ECL 60 sec followed by a 45 sec exposure). Other detection methods will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-thyroglobulin-antibody-m00359-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00359-tg-primary-antibodies-wb-testing-1.jpgAnti-Thyroglobulin TG Monoclonal AntibodyWestern blot using Boster Immunochemical's Mouse Mab-anti-Thyroglobulin antibody. Separation was achieved under reducing conditions using a pre-cast 5% Tris-HCl gel from Bio-Rad Laboratories. This antibody recognizes a single 330 kDa band corresponding to human thyroglobulin (left lane 3 µg, right lane 3 ng) as confirmed by the position of molecular weight markers (not shown). A 1:400 dilution of Mab anti-Thyroglobulin was used for 2h followed by detection using a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-gata4-antibody-m00499-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00499-gata4-primary-antibodies-wb-testing-1.jpgAnti-GATA4 Monoclonal AntibodyWestern Blot of Mouse anti-GATA4 Antibody. Lane 1: CCRF-CEM Lysate. Load: 25 µg per lane. Primary antibody: GATA-4 antibody at 1:500 overnight at 4°C. Secondary antibody: anti-mouse IgG HRP antibody at 1:40,000 for 30 min at RT. Block: 30 min at RT. Predicted/Observed size: 44.5 kDa, 44.5 kDa for GATA4. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-pms2-antibody-m01028-boster.html2026-03-24T05:06:09+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01028-pms2-primary-antibodies-wb-testing-1.jpgAnti-PMS2 Monoclonal AntibodyWestern blot analysis is shown using Boster's Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. Personal communication Morphotek Inc.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01028-pms2-primary-antibodies-diagram-testing-3.jpgAnti-PMS2 Monoclonal AntibodyRelevant protein domains for human PMS2. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lgr4-antibody-m02134-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02134-lgr4-primary-antibodies-ihc-testing-1.jpgAnti-LGR4/Gpr48 Monoclonal AntibodyBoster's anti-LGR4 monoclonal antibody was used diluted to 5 µg/ml to detect LGR4 staining at the membrane of cells in various human tissues. A. Brain cerebellum. B. Pancreas islet. Strongly positive staining is noted in subsets of cells within the islets of Langerhans. Moderately positive staining was observed in Purkinje and Golgi neurons of the cerebellum, adrenal medulla, neuroendocrine cells, hepatocytes, lung macrophages, seminiferous tubules and Leydig cells of the testis. Faintly to moderately positive staining was also observed in cardiac myocytes and renal tubules, granulocytes, and subsets of lymphocytes. Some elastin background staining is noted. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-keratin-antibody-m07078-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07078-krt6c-primary-antibodies-ihc-testing-4.jpgAnti-Keratin KRT6C Monoclonal AntibodyImmunohistochemistry of Mouse anti-Keratin antibody. Tissue: human prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Keratin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Keratin as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07078-krt6c-primary-antibodies-if-testing-1.jpgAnti-Keratin KRT6C Monoclonal AntibodyImmunofluorescence Microscopy of Boster Immunochemical's Anti-Keratin antibody was used with Boster's DyLight™ 488 goat anti-mouse [shown in green] to detect Keratin by Immunofluorescence. In the same experiment, Boster's polyclonal Anti-HDAC-1 antibody was used with Atto425 Anti-Rabbit IgG [shown in red] to detect HDAC-1. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USAhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07078-krt6c-primary-antibodies-wb-testing-2.jpgAnti-Keratin KRT6C Monoclonal AntibodyWestern blot using Boster Immunochemical's Mouse Anti-Keratin antibody. This antibody recognizes a single 56kDa band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximately 100ng of keratin from human epidermis was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Mouse IgG [H&L] and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07078-krt6c-primary-antibodies-if-testing-3.jpgAnti-Keratin KRT6C Monoclonal AntibodyImmunofluorescence using Boster Immunochemical's Mouse Anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 µm where the image is taken near the bottom of the cell. Use FITC conjugated Goat-a-Mouse IgG [H&L] at 1:2,000 dilution for detection. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mtbp-antibody-m08842-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08842-mtbp-primary-antibodies-wb-testing-1.jpgAnti-MTBP Monoclonal AntibodyWestern blot using Boster's anti-MTBP antibody shows detection of a band ~110 kDa corresponding to human MTBP (arrowhead). Lanes represent human 293 cell lysates with (+) and without (-) transfection with a full-length human expression MTBP construct. The transfected cell extract was diluted 30 fold in extract lacking transfected MTBP. Proteins were separated by SDS-PAGE and transferred onto PDVF membrane. After blocking, the membrane was probed with the primary antibody diluted to 1:500 for 2h at room temperature followed by detection using a Lumi-LightPlus Western Blotting Kit (Roche). https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-bin3-antibody-m11817-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11817-bin3-primary-antibodies-wb-testing-1.jpgAnti-BIN3 Monoclonal AntibodyWestern Blot of Mouse Anti-BIN3 antibody. Lane 1: MEF cells wild type. Lane 2: MEF cells null mice. Load: 35 µg per lane. Primary antibody: BIN3 antibody overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 30 kDa for BIN-3. Other band(s): non-specific.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m11817-bin3-primary-antibodies-wb-testing-3.jpgAnti-BIN3 Monoclonal AntibodyWestern Blot of Mouse anti-BIN3 antibody. Lane 1: MEF lysate BIN3 +/-. Lane 2: MEF lysate BIN3 -/-. Load: 35 µg per lane. Primary antibody: BIN-3 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 31 kDa for BIN 3. Other band(s): * non-specifics. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-aurora-b-phospho-pt232-antibody-p00762-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00762-aurkb-primary-antibodies-ihc-testing-2.jpgAnti-Phospho-Aurora B pT232 Aurkb AntibodyImmunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody. Tissue: human intestine pH9 (A) at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AuroraB pT232 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AuroraB pT232 is cytoplasmic. Staining: AuroraB pT232 as precipitated brown signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00762-aurkb-primary-antibodies-ihc-testing-3.jpgAnti-Phospho-Aurora B pT232 Aurkb AntibodyImmunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody. Tissue: human placenta pH9 (A) at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AuroraB pT232 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AuroraB pT232 is cytoplasmic. Staining: AuroraB pT232 as precipitated brown signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00762-aurkb-primary-antibodies-wb-testing-1.jpgAnti-Phospho-Aurora B pT232 Aurkb AntibodyWestern Blot shows detection of Aurora B protein at 39 kDa (predicted band size). All lanes : Aurora B (phospho T232) antibody diluted 1:500. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) and with the phosphopeptide immunogen. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-ect2-phospho-t790-antibody-p01862-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p01862-ect2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-ECT2 T790 AntibodyWestern blot using Boster's affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-mark2-phospho-t595-antibody-p02117-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02117-mark2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-MARK2 T595 AntibodyWestern Blot of Rabbit anti-MARK2pT595 antibody. Lane 1: wild type Jurkat cells. Lane 2: wild type Jurkat cells stimulated with CD3/CD28 (T-cell receptor stimulation). Load: 35 µg per lane. Primary antibody: MARK2 pT595 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~83 kDa for MARK2pT595.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02117-mark2-primary-antibodies-wb-testing-2.jpgAnti-Phospho-MARK2 T595 AntibodyWestern Blot of Rabbit anti-MARK2pT595 antibody. Lane 1: 293T cells untransfected. Lane 2: 293T cells transfected with MARK2 (EMK1) and PKC-zeta. Lane 3: 293T cells transfected with MARK2 (EMK1). Load: 35 µg per lane. Primary antibody: MARK2pT595 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~83kDa kDa for MARK2 pT595. Other band(s): MARK2pT595 splice variants and isoforms. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-apc1-phospho-s355-antibody-p03471-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03471-anapc1-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-APC1 S355 ANAPC1 AntibodyBoster's affinity purified anti-APC1 pS377 antibody was used at 5.0µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive cytoplasmic and occasional nuclear staining of pancreatic carcinoma cells at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03471-anapc1-primary-antibodies-wb-testing-2.jpgAnti-Phospho-APC1 S355 ANAPC1 AntibodyWestern blot using Boster's Affinity Purified anti-APC1 pS377 antibody shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. While some phosphorylated APC1 is present in untreated cell, the amount of phosphorylated protein is increased in cell preparations arrested in mitosis. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-glycogen-synthase-1-phospho-s641-antibody-p03512-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03512-gys1-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-Glycogen Synthase 1 S641 GYS1 AntibodyImmunohistochemistry with Anti-Glycogen Synthase antibody. Tissue: Human Prostate. Fixation: formalin-fixed, paraffin-embedded tissue. Antigen retrieval: heat-induced. Primary antibody: 5 µg/ml. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03512-gys1-primary-antibodies-wb-testing-2.jpgAnti-Phospho-Glycogen Synthase 1 S641 GYS1 AntibodyAffinity Purified Phospho-specific pS641 antibody to human muscle Glycogen Synthase (GS). Lane 1: mouse cardiac myocyte lysate mock treated. Lane 2: mouse cardiac myocyte lysate insulin treated at 10nM for 15'. Lane 3: mouse cardiac myocyte lysate insulin treated at 100nM for 15'. Lane 4: mouse cardiac myocyte lysate insulin treated at 1nM for 15'. Lane 5: mouse cardiac myocyte lysate mock treated. Lane 6: mouse cardiac myocyte lysate CLA treated at 4nM for 45'. Lane 7: mouse cardiac myocyte lysate CLA treated at 20nM for 45'. Lane 8: mouse cardiac myocyte lysate CLA treated at 100nM for 45'. Load: 12µL. Primary Antibody: pS641 at 1:1000. Secondary Antibody: HRP conjugated Gt-a-Rabbit IgG (611-103-122) at 1:5,000 dilution preceded color development using Amersham's substrate system. Other detection methods will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03512-gys1-primary-antibodies-pathway-testing-3.jpgAnti-Phospho-Glycogen Synthase 1 S641 GYS1 AntibodyDiagram of glycogen synthase as a component of insulin signal transduction pathways. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-apc6-phospho-t580-antibody-p04573-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p04573-cdc16-primary-antibodies-wb-testing-1.jpgAnti-Phospho-APC6 T580 CDC16 AntibodyWestern blot using Boster's Affinity Purified anti-APC6 pT580 antibody shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate . Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures . Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-arhgap22-phospho-s397-antibody-p10197-boster.html2026-03-24T05:06:10+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/1/p10197-arhgap22-primary-antibodies-wb-testing-1.jpgAnti-Phospho-ARHGAP22 S397 AntibodyWestern Blot of Rabbit anti-ARHGAP22 pS22 antibody. Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutation. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO O/N at 4°C. Predicted/Observed size: 68 kDa for ARHGAP22. Other band(s): Unmodified ARHGAP22. ARHGAP22-pS22 antibody recognizes the S397>A mutation, not the S22>mutation; ARHGAP22 pS397 recognizes the pS22>A mutation, not the pS397>A mutation; Confirms the specificity of each ARHGAP22 phospho specific antibody. https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-gsk3-beta-rabbit-monoclonal-antibody-m00791-boster.html2026-03-24T05:06:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-gsk3-beta-primary-antibodies-wb-testing-1.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyWestern blot analysis of GSK3 beta using anti-GSK3 beta antibody (M00791). <br>Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1: human 293T whole cell lysates,<br> Lane 2: human Hela whole cell lysates,<br> Lane 3: human U87 whole cell lysates,<br> Lane 4: human MCF-7 whole cell lysates,<br> Lane 5: rat PC-12 whole cell lysates,<br> Lane 6: rat C6 whole cell lysates,<br> Lane 7: mouse RAW264.7 whole cell lysates,<br> Lane 8: mouse Neuro-2a whole cell lysates.<br>After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 beta antigen affinity purified monoclonal antibody (M00791) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GSK3 beta at approximately 47 kDa. The expected band size for GSK3 beta is at 47 kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-fphar-14-1038039-g004.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyWECP activated Akt/GSK3β/β-Catenin signaling pathway in DPCs. DPCs were incubated with WECP (40, 80 and 160 μg/mL) in culture medium for 24 h. (A) Akt and GSK3β phosphorylation levels were detected in DPCs after WECP treatment. (B) Effects of WECP treatment on β-Catenin and Wnt10b protein expression levels in DPCs. (C) Transcriptional expression of LEF1, IGF1, and VEGF in DPCs detected using RT-PCR. Data are presented as means ± SD of three independent replicates. * p < 0.05, ** p < 0.01 vs. control group.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9986263/&orig_host=www.ncbi.nlm.nih.gov'>36891275</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-fphar-14-1038039-g005.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyWECP activated Akt/GSK3β/β-Catenin signaling pathway in denuded mouse skin. (A) Effects of WECP and finasteride treatments on Akt and GSK3β protein phosphorylation in mouse skin. (B) Effects of WECP and finasteride treatments on β-Catenin and Wnt10 translational expression in mouse skin. (C) Effects of WECP and finasteride treatments on transcriptional expression of β-Catenin, Wnt10b, Wnt5a, LEF1, VEGF, and IGF1 in mouse skin. Data are means ± SD of three independent replicates. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group. WECPL, WECPH, and Fin represent low-dose WECP, high-dose WECP, and finasteride treatment, respectively.<br><b>Index in PubMed under a CC BY license. PMID: <a href='https://misuse.ncbi.nlm.nih.gov/error/abuse.shtml?orig_args=/pmc/articles/PMC9986263/&orig_host=www.ncbi.nlm.nih.gov'>36891275</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-ihc8.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-ihc9.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human small cell lung cancer , using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-ihc10.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human pancreatic cancer, using the Antibody at 1:400 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-ihc7.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:100 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-ihc.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human colon carcinome, using GSK3 beta Antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-icc1.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyImmunofluorescent analysis using the Antibody at 1:50 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00791-if.jpgAnti-GSK3 beta Rabbit Monoclonal AntibodyImmunofluorescent analysis of Hela cells, using GSK3 beta Antibody . https://www.bosterbio.com/products/primary-antibodies/recombinant-antibodies/anti-lamp2a-rabbit-monoclonal-antibody-m01573-boster.html2026-03-24T05:06:15+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-wb7.jpgAnti-LAMP2a Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-ihc8.jpgAnti-LAMP2a Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human small cell lung cancer , using the Antibody at 1:600 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-wb8.jpgAnti-LAMP2a Rabbit Monoclonal AntibodyAll lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-wb.jpgAnti-LAMP2a Rabbit Monoclonal AntibodyWestern blot analysis of LAMP2 expression in JAR cell lysate.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-ihc7.jpgAnti-LAMP2a Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:200 dilution.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m01573-ihc.jpgAnti-LAMP2a Rabbit Monoclonal AntibodyImmunohistochemical analysis of paraffin-embedded human liver cancer, using LAMP2 Antibody. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-hepatitis-virus-a59-nonstructural-protein-9-antibody-m19765-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m19765-1a-primary-antibodies-if-testing-1.jpgAnti-Hepatitis Virus A59 Nonstructural Protein 9 1a Monoclonal AntibodyImmunofluorescence microscopy using Boster Immunochemical's anti-MHV-A59 nsp9 antibody, 6-h post infection in mouse L cells. Cells were fixed in 3% para-formaldehyde. For detection Cy2 conjugated Goat-anti-Mouse IgG MX10 (610-111-121) was used. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/1/m19765-1a-primary-antibodies-wb-testing-2.jpgAnti-Hepatitis Virus A59 Nonstructural Protein 9 1a Monoclonal AntibodyWestern blotting using Boster's anti-MHV-A59 nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no cross-reactivity with SARS-CoV-infected Vero cells. Negative controls (lanes 2 and 4) show no staining. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-il-7-receptor-alpha-chain-phospho-y449-antibody-p02222-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02222-il7r-primary-antibodies-wb-testing-1.jpgAnti-Phospho-IL-7 Receptor Alpha Chain Y449 AntibodyWestern Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Lane 1: thymocyte D1 cells. Lane 2: thymocyte D1 cells treated with IL7 (50 ng/ml). IP: with anti-Phosphotyrosine conjugated to Protein G agarose. Primary antibody: IL7pY449 antibody at 1:10000 for overnight at 4°C. Secondary antibody: HRP-conjugated goat anti-rabbit antibody at 1:10,000 for 45 min at RT and ECL detection. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p02222-il7r-primary-antibodies-wb-testing-3.jpgAnti-Phospho-IL-7 Receptor Alpha Chain Y449 AntibodyWestern Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Load: thymocyte D1 cells treated with or without IL-7 (50 ng/ml) for 20 min. Primary antibody: IL7pY449 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55 kDa, ~64 kDa for IL7pY449. Other band(s): unspecifics. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il-17f-biotin-conjugated-antibody-b02062-1-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b02062-1-il17f-primary-antibodies-ihc-testing-1.jpgAnti-Human IL-17F Biotin Conjugated Monoclonal AntibodyImmunohistochemistry of Mouse Anti-IL-17F antibody. Tissue: human colon tissue. Fixation: formalin-fixed, paraffin-embedded. Primary antibody: isotype control (top left), Mouse Anti-IL-17F antibody (right and bottom left) at 5 ug/mL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b02062-1-il17f-primary-antibodies-wb-testing-2.jpgAnti-Human IL-17F Biotin Conjugated Monoclonal AntibodyWestern Blot of Mouse Anti-IL-17F antibody. Lane 1: human full length recombinant IL-17F protein. Lane 2: mouse full length recombinant IL-17F protein. Lane 3: rat full length recombinant IL-17F protein. Load: 20 ng/lane. Primary antibody: Anti-IL-17F antibody (209-301-B31) at 0.5ug/mL for overnight at 4°C. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-human-il-17e-biotin-conjugated-antibody-b04429-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b04429-il25-primary-antibodies-fcm-testing-1.jpgAnti-Human IL-17E Biotin Conjugated IL25 Monoclonal AntibodyAnti-IL-17E Antibody - Flow Cytometry Cells: PC3 cells Primary antibody: 1 ug of IL-17E (red), control (green) Secondary antibody: anti-mouse IgG PE conjugated secondary antibodyhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b04429-il25-primary-antibodies-wb-testing-2.jpgAnti-Human IL-17E Biotin Conjugated IL25 Monoclonal AntibodyAnti-IL-17E Biotin Conjugated Antibody - Western Blot. Western Blot of Mouse anti-IL-17E antibody. Load: full length recombinant protein. Primary Antibody: anti-IL-17E antibody (209-301-C58) at 0.5 ug/mL. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-smad3-phospho-s423-phospho-s425-antibody-p00059-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00059-smad3-primary-antibodies-wb-testing-2.jpgAnti-Phospho-SMAD3 S423/S425 AntibodyWestern Blot of Rabbit anti-SMAD3 pS423 pS425 antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: A549 Lysate . Lane 7: LNCap Lysate . Lane 8: MOLT-4 Lysate . Lane 9: Ramos Lysate . Lane 10: Raji Lysate . Lane 11: A-172 Lysate . Lane 12: NIH/3T3 Lysate . Load: 10 µg per lane. Primary antibody: SMAD3 pS423 pS425antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 35 kDa for SMAD3 pS423 pS425.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00059-smad3-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-SMAD3 S423/S425 AntibodyBoster's affinity purified anti-Smad3 pS423 pS425 antibody was used at 2.5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong nuclear staining in the majority of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00059-smad3-primary-antibodies-wb-testing-3.jpgAnti-Phospho-SMAD3 S423/S425 AntibodyWestern blot using Boster's affinity purified anti-Smad3 pS423 pS425 antibody shows detection of endogenous Smad3 in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF (+lanes) for 1 hour. This reagent recognizes phosphorylated Smad3 and has negligible reactivity against non-phosphorylated Smad3 protein. Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-ubiquitin-activating-enzyme-e1-antibody-a02810-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02810-uba1-primary-antibodies-ihc-testing-1.jpgAnti-Ubiquitin Activating Enzyme E1 UBA1 AntibodyBoster's Affinity Purified anti-Ubiquitin Activating Enzyme antibody was used at a 10 µg/ml to detect UBE1 in a variety of tissues including adrenal, breast, colon (epithelium), kidney, liver, lung (respiratory epithelium), ovary (oocyte and endothelium), pancreas (islet and exocrine), placenta, prostate (epithelium), skin (epithelium), spleen (lymphocytes), stomach (chief), testis, thymus, tonsil, and uterus (glandular, stroma). In many cells a punctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining. This image shows UBE1 staining of human lung tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpan Biosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02810-uba1-primary-antibodies-wb-testing-2.jpgAnti-Ubiquitin Activating Enzyme E1 UBA1 AntibodyWestern blot using Boster's purified anti-Ubiquitin Activating Enzyme (E1) antibody shows detection of a band at ~118 kDa corresponding to UBE1 (lane 1 800 nm channel). Approximately 35µg of an A431 whole cell lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature. Molecular weight markers are shown in lane 2 (700 nm channel). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-fibroblast-activation-protein-antibody-a00422-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00422-fap-primary-antibodies-elisa-testing-2.jpgAnti-Fibroblast Activation Protein FAP AntibodyELISA results of purified Rabbit anti-Fibroblast Activation Protein (FAP) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00422-fap-primary-antibodies-wb-testing-1.jpgAnti-Fibroblast Activation Protein FAP AntibodyWestern blot using Boster's affinity purified anti-FAP antibody shows detection of FAP protein in whole cell lysates from FAP expressing HEK cells (lane 4) but not control HEK cells (lane 3).  Specific band staining is blocked when the primary antibody is pre-incubated with immunizing peptide (lanes 1 and 2 respectively).  The band at ~90 kDa, indicated by the arrowhead, corresponds to the expected molecular weight of transfected FAP. Primary antibody was used at 1:1,000.  Personal communication, S.Kim, NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-selenophosphate-synthetase-1-antibody-p10102-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/1/p10102-sephs1-primary-antibodies-wb-testing-1.jpgAnti-Selenophosphate Synthetase 1 SEPHS1 AntibodyWestern blot using Boster's anti-SPS1 antibody shows detection of endogenous SPS1 in NIH3T3 cell (lane 1), mouse kidney, and liver tissue lysates (lanes 3-4). No signal is seen in NIH3T3 cells after pre-treatment with SPS1 siRNA (lane 2). SPS1 control (lane 5-6). Negligible cross-reactivity is seen against recombinant SPS2 (lane 7). The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-selenophosphate-synthetase-2-antibody-p11872-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/1/p11872-sephs2-primary-antibodies-wb-testing-1.jpgAnti-Selenophosphate Synthetase 2 SEPHS2 AntibodyWestern blot using Boster's Protein A purified anti-SPS2 antibody shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-procalcitonin-4c8-h6-d4-antibody-m02352-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m02352-calca-primary-antibodies-wb-testing-1.jpgAnti-Procalcitonin (4C8.H6.D4) Calca Monoclonal AntibodyWestern Blot of Mouse anti-Procalcitonin Antibody. Lane 1: rProcalcitonin. Load: 50 ng. Primary antibody: Mouse anti-Procalcitonin at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 30 min at RT. Predicted/Observed size: 13.9 kDa, 13.9 kDa https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-cenexin-1-phospho-s796-antibody-p05599-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p05599-odf2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-Cenexin-1 S796 ODF2 AntibodyWestern Blot of Rabbit Anti-Cenexin-1 pS796. Lane 1: MW. Lane 2: Human Semen Lysate. Lane 3: MCF7 WCL . Lane 4: Molt 4 WCL . Lane 5: HeLa WCL . Load: 10µg per lane. Blocking: . Primary Antibody: Anti-Cenexin-1 pS796 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit HRP 1:40,000 diluted in for 30 minutes at RT. Expect: 93kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p05599-odf2-primary-antibodies-elisa-testing-2.jpgAnti-Phospho-Cenexin-1 S796 ODF2 AntibodyELISA of Rabbit Anti-Cenexin-1 pS796 Antibody. Antigen: BSA conjugates of Cenexin-1 pS796 (purple) and S796 (blue). Coating amount: 0.1 µg per well. Primary antibody: Cenexin-1 pS796 Lot 35048 at 5 µg/mL. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8,500. Substrate: TMB . https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-alkaline-phosphatase-antibody-p06115-boster.html2026-03-24T05:06:16+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p06115-alp1-primary-antibodies-wb-testing-1.jpgAnti-Alkaline Phosphatase ALPI AntibodyWestern Blot of Rabbit Anti-Alkaline Phosphatase Primary Antibody. Molecular Weight Marker. Lane 1: Alkaline Phosphatase. Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase primary antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: for 30 min at RT. Predicted/Observed size: 58 kDa for Alkaline Phosphatase.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p06115-alp1-primary-antibodies-wb-testing-2.jpgAnti-Alkaline Phosphatase ALPI AntibodyBoth the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (611-1302) diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-sh3bp2-phospho-s427-antibody-p04008-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p04008-sh3bp2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-SH3BP2 S427 AntibodyWestern blot analysis is shown using Boster's Affinity Purified anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates.  The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B).  Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein.  The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.  Personal communication Steven Pelech, Kinexus Inc. https://www.bosterbio.com/products/primary-antibodies/anti-apolipoprotein-a-i-antibody-a00717-1-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00717-1-apoa1-primary-antibodies-ihc-testing-3.jpgAnti-APOLIPOPROTEIN A-I APOA1 AntibodyBoster's anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00717-1-apoa1-primary-antibodies-sds-page-testing-1.jpgAnti-APOLIPOPROTEIN A-I APOA1 AntibodyCoomassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using Boster's anti-Human apoLipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-e2f-1-phospho-s364-antibody-p00257-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00257-e2f1-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-E2F-1 S364 AntibodyBoster's Affinity Purified anti- E2F-1 pS364 antibody was used at a 10 µg/ml to detect nuclear and occasionally cytoplasmic signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear staining between individual tumors, with some tumors showing strong staining. This image shows E2F-1 pS364 staining of human breast carcinoma. Tissue was formalin-fixed and paraffin embedded.  Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00257-e2f1-primary-antibodies-wb-testing-3.jpgAnti-Phospho-E2F-1 S364 AntibodyWestern blot using Boster's Affinity Purified anti-E2F-1 pS364 antibody shows detection of a band at ~47 kDa corresponding to phosphorylated E2F-1 in induced cell lysates.  Panel A shows reactivity using a control antibody reactive to all forms of E2F (arrowheads).  Panel B shows specific reactivity against phosphorylated E2F-1 (arrowheads) using our anti-E2F-1 pS364 antibody.  Lysates are as follows: CRE/E2F-1 are CRE cells derived from mouse NIH3T3 line transfected with human E2F-1, NIH-3T3 used as a negative control, and MDA-MB-231 cells are a human breast cancer line.  As indicated each lysate was prepared from untreated cells and cells treated with 2 µM Doxorubicin used as a DNA damaging agent.  In addition the MDA-MB-231 cells were also treated with genistein, a mild DNA damaging agent.  The figure shows the same membrane first probed with the anti-E2F-1 pS364 antibody used at a 1:250 dilution, then stripped and re-probed with the pan E2F antibody used as a positive control.  The positive control antibody clearly shows an E2F-1 band in all human cell lines, but not mouse cells.  Treatment with doxorubicin increases the expression of E2F-1 as shown in Panel A.  After film development, images were overlapped to confirm that specific anti-E2F-1 pS364 staining shown treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A.   Blots can be processed with HRP conjugated Gt-a-Rabbit IgG MX10 611-103-122 for 45 min at room temperature for ECL detection.  Personal Communication, XiaoHe Yang, Univ. Oklahoma. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-asap1-phospho-y782-antibody-p03848-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03848-asap1-primary-antibodies-if-testing-1.jpgAnti-Phospho-ASAP1 Y782 AntibodyImmunofluorescent microscopy using Boster's Affinity Purified anti-ASAP1 pY782 antibody shows detection of phosphorylated ASAP1 present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p03848-asap1-primary-antibodies-ihc-testing-3.jpgAnti-Phospho-ASAP1 Y782 AntibodyBoster's affinity purified anti-ASAP1 pY782 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpan Biosciences, Seattle, WA. https://www.bosterbio.com/products/primary-antibodies/anti-hdac-1-c-terminus-antibody-a00256-1-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-1-hdac1-primary-antibodies-ihc-testing-1.jpgAnti-HDAC-1 (C-terminus) AntibodyImmunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 precipitated purple with blue counterstain. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-1-hdac1-primary-antibodies-wb-testing-2.jpgAnti-HDAC-1 (C-terminus) AntibodyWestern Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: 293 whole cell lysate . Load: 35 µg per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-1-hdac1-primary-antibodies-ihc-testing-3.jpgAnti-HDAC-1 (C-terminus) AntibodyImmunohistochemistry of Rabbit Anti-HDAC-1 Antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 as brown color indicates presence of protein, blue color shows cell nuclei.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-1-hdac1-primary-antibodies-if-testing-4.jpgAnti-HDAC-1 (C-terminus) AntibodyImmunofluorescence Microscopy of Rabbit Anti-HDAC-1 antibody. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: HDAC-1 is nuclear. Staining: HDAC-1 was used with Atto 425 (shown in red). Anti-Keratin monoclonal antibody was used with Dylight 488 (shown in green) to detect Keratin. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-1-hdac1-primary-antibodies-if-testing-5.jpgAnti-HDAC-1 (C-terminus) AntibodyImmunofluorescence Microscopy of Rabbit anti-HDAC1 Antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum. Primary antibody: HDAC1 antibody at 4 µg/mL for 1 h at RT. Secondary antibody: rabbit secondary antibody at 0.2 µg/mL for 45 min at RT. Localization: HDAC1 is nuclear. Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00256-1-hdac1-primary-antibodies-wb-testing-6.jpgAnti-HDAC-1 (C-terminus) AntibodyWestern Blot of Rabbit Anti-HDAC-1 Antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 µg per lane. Primary antibody: HDAC-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 55kDa for HDAC-1. https://www.bosterbio.com/products/primary-antibodies/anti-hdac-2-c-terminus-antibody-a00325-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00325-hdac2-primary-antibodies-wb-testing-1.jpgAnti-HDAC-2 (C-terminus) AntibodyWestern Blot of Rabbit anti-HDAC2 antibody. Lane 1: mouse brain extract. Lane 2: mouse brain extract blocked with peptide. Load: 4 µg per lane. Primary antibody: HDAC2 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 59 kDa for HDAC2. Other band(s): ~45kDa. https://www.bosterbio.com/products/primary-antibodies/anti-hdac-7-n-terminus-antibody-a01913-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01913-hdac7-primary-antibodies-wb-testing-1_1.jpgAnti-HDAC-7 (N-terminus) AntibodyWestern Blot of Rabbit anti-HDAC7 antibody. Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 µg per lane. Primary antibody: HDAC7 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~103kDa/ ~103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms ~70 and 40kDa. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-atm-phospho-s1981-antibody-p00014-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00014-atm-primary-antibodies-pathway-testing-2.jpgAnti-Phospho-ATM S1981 AntibodySchematic of ATM induction by DNA damage.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00014-atm-primary-antibodies-wb-testing-1.jpgAnti-Phospho-ATM S1981 AntibodyWestern Blot of Sheep Anti-ATM pS1981 polyclonal antibody. Lane 1: untreated MCF-7 cell lysate . Lane 2: Hydrogen Peroxide stimulated MCF-7 Whole Cell Lysate . Load: 35 µg per lane. Primary antibody: ATM pS1981 antibody at 1:1000 for 1 h at room temperature. Secondary antibody: IRDye™800 conjugated Donkey anti-Sheep IgG secondary antibody at 1:5,000 for 1h at room temperature. Block: 5% BLOTTO (B501) overnight at 4°C. Predicted/Observed size: 370 kDa, ATM (370 kDa) is indicated by an arrow. Other band(s): ATM splice variants and isoforms. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-mdm2-phospho-s185-antibody-p00054-boster.html2026-03-24T05:06:17+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00054-mdm2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-MDM2 S185 AntibodyAffinity Purified Anti-MDM2 pS185 (Rabbit) is shown to detect a 102 kDa band  (arrow) corresponding to phosphorylated mouse MDM2 present in a 293T whole cell lysate.   Cells were serum-starved for 24 hours prior to harvest.  Approximately 20 µg of lysate was loaded per lane for SDS-PAGE.  Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest.  Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-creb-phospho-s133-antibody-p00577-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00577-creb1-primary-antibodies-ihc-testing-1.jpgAnti-Phospho-CREB S133 CREB1 AntibodyBoster's affinity purified anti-CREB pS133 antibody was used at 20µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate to strong nuclear staining of tonsillar lymphocytes. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00577-creb1-primary-antibodies-wb-testing-2.jpgAnti-Phospho-CREB S133 CREB1 AntibodyAnti-CREB pS133 was used to detect phosphorylated CREB by western blot at ~46kDa.  Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography.  An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP.  Western blot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form.  Pan reactive CREB (Boster # 100-401-195) reacts equally with both non-phosphorylated and phosphorylated CREB (not shown).  Detection occurs using a 1:500 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL.  Film exposure was approximately 1’.  Other detection systems will yield similar results. Personal Communication, Boss, J., Emory University School of Medicine, Atlanta, GA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00577-creb1-primary-antibodies-wb-testing-3.jpgAnti-Phospho-CREB S133 CREB1 AntibodyAnti-CREB pS133 was used to detect phosphorylated CREB by western blot. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Western blot of indicated amounts (100ng, 50ng, 25ng, 12.5ng) of control (-) and in-vitro phosphorylated CREB (P) were loaded to show that the antibody reacts specifically with the phosphorylated form. Blots were blocked in 5% milk in TBS+0.1% Tween-20 (TBST-M) overnight at 4°C. Detection occurs using a 1:500 dilution of antibody diluted in TBST-M and incubated at room temperature with rocking for 1 hour. Blots were rinsed 6X with TBST and incubated with goat anti-rabbit-HRP at 1:5000 in TBST-M at room temperature for 45 min. Blots were again rinsed 6X with TBST and then processed using ECL reagent (Amersham) according to manufacturer's instructions. Exposure time: 1 min using Kodak Biomax MR film. Personal Communication, R. Screaton, The Salk Institute for Biological Studies. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-collagen-type-ii-antibody-a00517-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00517-col2a1-primary-antibodies-ihc-testing-1.jpgAnti-Collagen Type II COL2A1 AntibodyBoster anti collagen II antibody (600-401-104 Lot 26014, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Image provided courtesy of LifeSpan Biosciences, Seattle, WA https://www.bosterbio.com/products/primary-antibodies/anti-collagen-type-iv-antibody-a01411-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01411-collagen-iv-primary-antibodies-ihc-testing-1_1.jpgAnti-Collagen Type IV COL4A1 AntibodyBoster anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WAhttps://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01411-collagen-iv-primary-antibodies-ihc-testing-2_1.jpgAnti-Collagen Type IV COL4A1 AntibodyImmunohistochemistry results of Rabbit Anti-Collagen Type IV Antibody. Tissue: human lung tissue. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type IV at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: basement membranes and vessels. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01411-collagen-iv-primary-antibodies-ihc-testing-3_1.jpgAnti-Collagen Type IV COL4A1 AntibodyImmunohistochemistry results of Rabbit Anti-Collagen Type IV Antibody. Tissue: human skeletal muscle cells. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type IV at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: cells surrounded by collagen IV fibers. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01411-collagen-iv-primary-antibodies-ihc-testing-4_1.jpgAnti-Collagen Type IV COL4A1 AntibodyImmunohistochemistry results of Rabbit Anti-Collagen Type IV Antibody. Tissue: human renal oncocytoma. Fixation: FFPE. Antigen Retrieval: HIER using Tris-EDTA-citrate buffer pH 7.8 for 5 min. Blocking: Peroxidase-Blocking Solution for 10 min. Primary Antibody: Anti-Collagen Type IV at 1:15 for 1 hr at 37 °C. Secondary Antibody: Dako REAL EnVision Detection Kit, Polymer-HRP, Rabbit/Mouse. Counterstain: Hematoxylin for 15 sec. Substrate: DAB-Chromogen, Rabbit/Mouse. Staining/Results: dense collagen IV positive membranes surrounding tumor cell nests. https://www.bosterbio.com/products/primary-antibodies/anti-collagen-type-iv-antibody-biotin-conjugated-b02908-boster.html2026-03-24T05:06:18+00:00daily0.9 https://www.bosterbio.com/products/primary-antibodies/phospho-specific-antibodies/anti-chk2-phospho-t68-antibody-p00277-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/p/0/p00277-chek2-primary-antibodies-wb-testing-1.jpgAnti-Phospho-CHK2 T68 CHEK2 AntibodyWestern blot using Boster's Affinity Purified anti-Chk2 pT68 antibody shows detection of a predominant band at ~60 kDa corresponding to phosphorylated Chk2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin.  Chk2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center. https://www.bosterbio.com/products/primary-antibodies/anti-hdac-5-internal-antibody-a01230-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01230-hdac5-primary-antibodies-wb-testing-1_1.jpgAnti-HDAC-5 (internal) AntibodyWestern Blot of Rabbit anti-HDAC5 antibody. Lane 1: mouse brain extract. Load: 5 µg per lane. Primary antibody: HDAC5 antibody at 0.2µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 124 kDa for HDAC5.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01230-hdac5-primary-antibodies-dot-blot-testing-3_1.jpgAnti-HDAC-5 (internal) AntibodyDot blot for Rabbit Anti-HDAC5 (internal) Antibody. Lane 1: HDAC-4 (internal). Lane 2: HDAC-5 . Lane 3: HDAC-5 (600-401-J68). Lane 4: HDAC-7 . Load: 100, 10, and 1 picomoles of peptide. Primary antibody: HDAC-5 antibody at 1:1000 for 45 min at 4°C. Secondary antibody: Dylight™488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01230-hdac5-primary-antibodies-wb-testing-2_1.jpgAnti-HDAC-5 (internal) AntibodyWestern Blot of Rabbit anti-HDAC5 antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: A549 Lysate . Lane 7: LNCap Lysate . Lane 8: MOLT-4 Lysate . Lane 9: Ramos Lysate . Lane 10: Raji Lysate . Lane 11: A-172 Lysate . Lane 12: NIH/3T3 Lysate . Load: 35 µg per lane. Primary antibody: HDAC5 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 124kDa for HDAC5. https://www.bosterbio.com/products/primary-antibodies/anti-collagen-type-i-antibody-biotin-conjugated-b00329-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b00329-col1a1-primary-antibodies-fcm-testing-1.jpgAnti-COLLAGEN Type I COL1A1 Antibody Biotin ConjugatedFlow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody (600-406-103). Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b00329-col1a1-primary-antibodies-fcm-testing-2.jpgAnti-COLLAGEN Type I COL1A1 Antibody Biotin ConjugatedFlow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody (600-406-103) at 5µg/mL for 45 min at 4°C. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT. Courtesy of D. Figueroa NIH. https://www.bosterbio.com/products/primary-antibodies/anti-alpha-1-trypsin-antibody-peroxidase-conjugated-h00720-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/0/h00720-serpina1-primary-antibodies-wb-testing-1.jpgAnti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase ConjugatedWestern Blot of Goat-anti-Alpha-1-Anti-Trypsin Antibody. Lane 1: molecular weight. Lane 2: Goat-anti-Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST. Load: 35 µg per lane under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Primary antibody: Alpha 1 Anti-Trypsin antibody at 1:400 for overnight at 4°C. Secondary antibody: DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT in buffer. Block: 2.5% Blotto, 2.5% BSA, 0.02% Tween over night at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): DyLight 488 Donkey anti-Mouse IgG (blue) and DyLight549 Donkey anti-Rabbit IgG (green) secondary antibodies.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/0/h00720-serpina1-primary-antibodies-wb-testing-2.jpgAnti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase ConjugatedWestern Blot of Goat Anti-ALPHA 1 ANTI TRYPSIN Antibody Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: ~1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: overnight at 4°C. Predicted/Observed size: 46.7kDa, ~55kDa for Alpha-1-AntiTrypsin. Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/0/h00720-serpina1-primary-antibodies-elisa-testing-3.jpgAnti-Alpha-1-Trypsin SERPINA1 Antibody Peroxidase ConjugatedELISA Results of Goat Anti-Alpha-1-Anti-Trypsin Peroxidase Conjugated Antibody. Each well was coated in duplicate with 1.0 µg of Alpha-1-Anti-Trypsin. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugated Stabilizer , Goat Anti-Alpha-1-Anti-Trypsin Peroxidase Conjugated Antibody and TMB substrate . https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-il-27-p28-antibody-m00857-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00857-il27-primary-antibodies-fcm-testing-1.jpgAnti-Mouse IL-27/P28 Monoclonal AntibodyMouse peritoneal macrophages were grown in culture for 24 hours, stimulated with 10ng/mL IFN gamma and 1ug/mL LPS for 14 hours and incubated for 4 hours with Bredfeldin A. Cells were harvested, washed, aliquoted 1x106 cells per sample, and fixed and permeabilized according to a standard protocol. Samples were stained with biotinylated primary anti-mouse p28 antibody at (0.1-10ug/mL primary antibody alongside negative controls of unstimulated cells and isotype controls. Cells were stained with 0.25ug/mL rat anti-mouse CD107b conjugated Alexa Fluor 647 and PHYCOERYTHRIN Conjugated secondary at 1:100 and analyzed by flow cytometry. Stimulated cells showed increase PE staining (horizontal axis) when compared with unstimulated cells.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00857-il27-primary-antibodies-wb-testing-2.jpgAnti-Mouse IL-27/P28 Monoclonal AntibodyAnti-IL-27/p28 antibody in western blot shows detection of recombinant mouse IL-27/p28. Recombinant protein (0.1 µg) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The expected band is approximately 26 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Boster's Anti-IL-27/p28 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rat IgG antibody diluted 1:40,000 in blocking buffer for 30 min at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cam-kinase-iv-antibody-a01905-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01905-camk4-primary-antibodies-ihc-testing-1_1.jpgAnti-CaM Kinase IV CAMK4 AntibodyImmunohistochemistry of Anti-CAMK4 antibody. Tissue: human brain cortex was formalin fixed and paraffin embedded. No pre-treatment of sample was required. Primary Antibody: Anti-CaM Kinase IV was diluted 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01905-camk4-primary-antibodies-wb-testing-2_1.jpgAnti-CaM Kinase IV CAMK4 AntibodyWestern blot using Boster's Anti-CaM Kinase IV antibody. Lane 1: Rat Brain, adult, WCL . Lane 2: Jurkat Whole Cell Lysate . Lane 3: Rat Brain, adult, WCL , preincubated with immunizing peptide. Lane 4: Jurkat Whole Cell Lysate , preincubated with immunizing peptide. Load: 35µg lysate/lane. Primary Antibody: Anti-CaM Kinase IV at 1:1,000 for 2hr at RT. Secondary Antibody: Goat Anti-Rabbit IgG IRDye800 at 1:10,000 for 45 mins at RT. Block: Fluorescent Buffer 30 mins at RT. Results: band ~52 kDa corresponding to CaM Kinase IV (arrowhead). CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. https://www.bosterbio.com/products/primary-antibodies/anti-pi4k-a-type-i-antibody-a07416-boster.html2026-03-24T05:06:18+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07416-pip5k1a-primary-antibodies-ihc-testing-1.jpgAnti-PI4K a, type I PIP5K1A AntibodyImmunohistochemistry of Rabbit anti-PIP5K1A antibody. Tissue: Tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PIP5K1A antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIP5K1A as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-alpha-tubulin-antibody-a08382-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08382-alpha-tubulin-primary-antibodies-if-testing-5.jpgAnti-alpha-Tubulin Tuba1b AntibodyImmunofluorescence microscopy of Rabbit Anti-alpha-Tubulin antibody using HeLa cells fixed with PFA. Anti-alpha-Tubulin Antibody was used at 1 µg/mL, O/N at 4⁰C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed at 2 ug/ml for 1 h at RT. Localization: TUBA1B is the major constituent of microtubules in the cytoplasm. Staining: Tubulin as green fluorescent signal with DAPI (blue) nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08382-alpha-tubulin-primary-antibodies-elisa-testing-3.jpgAnti-alpha-Tubulin Tuba1b AntibodyELISA results of purified Rabbit anti-alpha-Tubulin Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08382-alpha-tubulin-primary-antibodies-wb-testing-2.jpgAnti-alpha-Tubulin Tuba1b AntibodyWestern Blot of Rabbit anti-alpha-Tubulin antibody. Lane 1: HeLa WCL . Lane 2: NIH/3T3 WCL . Load: 10 µg per lane. Primary antibody: alpha-Tubulin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for alpha-Tubulin. Other band(s): N/A.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08382-alpha-tubulin-primary-antibodies-wb-testing-4.jpgAnti-alpha-Tubulin Tuba1b AntibodyWestern Blot of Rabbit anti-Alpha-Tubulin antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: LNCaP Lysate . Lane 7: A-172 Lysate . Lane 8: NIH/3T3 Lysate . Load: 35 µg per lane. Primary antibody: Alpha-Tubulin antibody at 1:2,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 50 kDa for Alpha-tubulin.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08382-alpha-tubulin-primary-antibodies-wb-testing-1.jpgAnti-alpha-Tubulin Tuba1b AntibodyWestern Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain . Lane 2: rat brain . Lane 3: A431 cells . Lane 4: Jurkat cells . Lane 5: HeLa cells . Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none. https://www.bosterbio.com/products/primary-antibodies/anti-beta-amyloid-antibody-a00081-2-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-if-testing-5.jpgAnti-Beta Amyloid APP AntibodyImmunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH. Anti-Beta Amyloid Antibody was used at 1 µg/mL, O/N at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed at 2 ug/ml for 1 h at RT. Localization: APP is a cell surface protein that rapidly becomes internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles. Colocalizes with other proteins in a vesicular pattern in cytoplasm and perinuclear regions. Staining: Amyloid beta as green fluorescent signal with DAPI (blue) nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-ihc-testing-2.jpgAnti-Beta Amyloid APP AntibodyImmunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-beta amyloid antibody 600-401-253 at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-wb-testing-8.jpgAnti-Beta Amyloid APP AntibodyWestern Blot of Rabbit Anti-Beta Amyloid Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: HEK293T Whole Cell Lysate . Lane 3: Mouse Brain Whole Cell Lysate . Lane 4: A-172 Whole Cell Lysate . Load: 10µg/lane. Primary Antibody: Anti-Beta Amyloid at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer . Predicted MW: ~40-50kDa. Observed MW: ~48kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-wb-testing-7.jpgAnti-Beta Amyloid APP AntibodyWestern Blot of Rabbit Anti-Beta Amyloid Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: HEK293T Whole Cell Lysate . Lane 3: Mouse Brain Whole Cell Lysate . Lane 4: A-172 Whole Cell Lysate . Lane 5: Daudi Whole Cell Lysate . Load: 10µg/lane. Primary Antibody: Anti-Beta Amyloid at 1:1000 overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer . Predicted MW: ~40-50kDa. Observed MW: ~48kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-if-testing-1.jpgAnti-Beta Amyloid APP AntibodyImmunohistochemical detection of beta Amyloid using Anti-Beta Amyloid Antibody on TG APP23 mouse brain cortex frozen sections. Anti-Beta Amyloid Antibody used at 1:200 and incubated for 2 hours in TBS/BSA with Tween and azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-ihc-testing-3.jpgAnti-Beta Amyloid APP AntibodyHuman Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-wb-testing-4.jpgAnti-Beta Amyloid APP AntibodyWestern Blot of Rabbit anti-Beta Amyloid antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: LNCaP Lysate . Lane 7: A-172 Lysate . Lane 8: NIH/3T3 Lysate . Load: 35 µg per lane. Primary antibody: Beta Amyloid antibody at 1:5,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for Beta Amyloid.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00081-2-app-primary-antibodies-elisa-testing-6.jpgAnti-Beta Amyloid APP AntibodyELISA results of purified Rabbit anti-Beta Amyloid Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate . https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mouse-il-17a-antibody-m00421-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00421-il17a-primary-antibodies-fcm-testing-2.jpgAnti-Mouse IL-17A Monoclonal AntibodyBoster monoclonal anti-IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN gamma over 8-10 days of culture. Cells were incubated for 15-20 minutes with addition of rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Boster Rat anti-mouse IL-17A monoclonal Antibody (210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00421-il17a-primary-antibodies-fcm-testing-1.jpgAnti-Mouse IL-17A Monoclonal AntibodyBoster monoclonal anti-IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN gamma over 8-10 days of culture. Cells were incubated for 15-20 minutes with addition of rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Boster Rat anti-mouse IL-17A monoclonal Antibody (210-501-B32) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for Boster’s monoclonal anti mouse IL-17A antibody (210-501-B32).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00421-il17a-primary-antibodies-wb-testing-3.jpgAnti-Mouse IL-17A Monoclonal AntibodyWestern Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Mouse IL-17A (RAT) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was diluted in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A. https://www.bosterbio.com/products/primary-antibodies/anti-mip-1-alpha-antibody-a00405-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00405-ccl3-primary-antibodies-wb-testing-1.jpgAnti-MIP-1 alpha CCL3 AntibodyAnti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E.coli. Recombinant truncated (0.1 µg, expect ~8kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Boster's, Inc. Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system. https://www.bosterbio.com/products/primary-antibodies/anti-rrm2b-p53r2-antibody-a03055-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03055-rrm2b-primary-antibodies-wb-testing-1.jpgAnti-RRM2B p53R2 AntibodyWestern blot using Boster's affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4: 293T transfected with ScRNA. Lane 5: 293T transfected with p53R2 SiRNA The band at 40.7 kDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9kDa. The highest band at 44.3 kDa corresponds to recombinant p53R2. Primary antibody was diluted to 1µg/mL and incubated overnight at 4°C. ECL detection was used. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-smac-diablo-antibody-a03790-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03790-diablo-primary-antibodies-wb-testing-2.jpgAnti-Smac/Diablo AntibodyWestern blot using anti-Smac detects a 26 kDa band when 1 µg of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 µg of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 µg each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 µM etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1’https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03790-diablo-primary-antibodies-wb-testing-1.jpgAnti-Smac/Diablo AntibodyAnti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1’. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-uplc1-asap3-antibody-a10444-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a10444-asap3-primary-antibodies-wb-testing-1.jpgAnti-Uplc1/Asap3 AntibodyWestern blot using Boster's protein A purified anti-UPLC1/ASAP3 antibody shows detection of UPLC1/ASAP3 in NIH/3T3 cells over-expressing the protein. Cell extracts (5 ug) were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with anti-UPLC1/ASAP3 at a 1:10,000 dilution. Personal Communication, Vi Luan HA, CCR-NCI, Bethesda, MD. 1 https://www.bosterbio.com/products/primary-antibodies/anti-fibronectin-antibody-biotin-conjugated-b00564-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b00564-fn1-primary-antibodies-ihc-testing-2.jpgAnti-Fibronectin FN1 Antibody Biotin ConjugatedImmunohistochemistry of Rabbit Anti-Fibronectin Antibody. Tissue: human kidney at pH6 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibronectin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b00564-fn1-primary-antibodies-ihc-testing-3.jpgAnti-Fibronectin FN1 Antibody Biotin ConjugatedImmunohistochemistry of Rabbit Anti-Fibronectin Antibody. Tissue: human kidney at pH9 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibronectin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b00564-fn1-primary-antibodies-ihc-testing-1.jpgAnti-Fibronectin FN1 Antibody Biotin ConjugatedImmunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). Primary antibody: 2ug/ml for 1 hour @ room T. Secondary antibody: Streptav. Conj. HRP 10 ug/ml circa 45 min. @ room T. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/anti-mip-3-alpha-antibody-biotin-conjugated-b00748-1-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/b/0/b00748-1-ccl20-primary-antibodies-wb-testing-1.jpgAnti-MIP-3 alpha Ccl20 Antibody Biotin ConjugatedWestern Blot of Biotin Conjugated Rabbit Anti-Human MIP-3α Antibody. Lane 1: Human MIP-3α. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human MIP-3α antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: 30 min at RT. Predicted/Observed size: 10 kDa, 10 kDa for Human MIP-3α. Other band(s): None. https://www.bosterbio.com/products/primary-antibodies/anti-plasminogen-antibody-peroxidase-conjugated-h00702-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/h/0/h00702-plg-primary-antibodies-wb-testing-1.jpgAnti-Plasminogen PLG Antibody Peroxidase ConjugatedBoster Goat anti Plasminogen antibody (200-101-208 lot 6571) was used to detect Plasminogen under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT. Images were collected using the BioRad VersaDoc System. https://www.bosterbio.com/products/primary-antibodies/anti-ppar-alpha-antibody-a00600-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-wb-testing-1.jpgAnti-PPAR alpha AntibodyWestern Blot of Rabbit anti-PPAR Alpha (N-terminal Specific) antibody. Lane M: Prestained Molecular Weight Markers. Lane 1: NIH/3T3 . Load: 10 µg per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting at RT for 30 min. Predicted/Observed size: ~50 kDa for PPAR Alpha.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-if-testing-7.jpgAnti-PPAR alpha AntibodyImmunofluorescence microscopy of Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary antibody only with NIH/3T3 cells. Anti-PPAR alpha antibody was used at 10 µg/mL, 1h at RT⁰. Secondary antibody: Anti-RABBIT IgG DyLight™ 488 Conjugated Preadsorbed at 5 ug/ml for 1 h at RT. Staining: PPAR as green fluorescent signal with DAPI (blue) nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-wb-testing-6.jpgAnti-PPAR alpha AntibodyWestern Blot of Rabbit anti-PPAR Alpha (N-terminal specific) antibody. Marker: Opal Pre-stained ladder . Lane 1: HEK293 lysate . Lane 2: HeLa Lysate . Lane 3: MCF-7 Lysate . Lane 4: Jurkat Lysate . Lane 5: A431 Lysate . Lane 6: LNCaP Lysate . Lane 7: A-172 Lysate . Lane 8: NIH/3T3 Lysate . Load: 35 µg per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1ug/mL overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 52 kDa for PPAR Alpha.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-elisa-testing-5.jpgAnti-PPAR alpha AntibodyELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-if-testing-4.jpgAnti-PPAR alpha AntibodyImmunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody. Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-wb-testing-8.jpgAnti-PPAR alpha AntibodyAffinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 µg of lysate was loaded per lane for SDS-PAGE.  Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-ihc-testing-2.jpgAnti-PPAR alpha AntibodyImmunohistochemistry using Boster's anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-ppara-primary-antibodies-ihc-testing-3.jpgAnti-PPAR alpha AntibodyImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) showing Boster's PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20°C. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-12276_2018_article_bfemm2017243_fig4_html.jpgAnti-PPAR alpha AntibodyPIK3R3 regulates the expression of PPARα. ( a ) Quantitative PCR (upper panel) analysis and western blot (bottom panel) analysis showing the expression level changes of Pparα in mice livers during HFD feeding. Data are expressed as the means±s.e.m. * P <0.05; ** P <0.01. ( b ) Immunohistochemistry analysis showing the expression level changes of hepatic Pparα in the livers of the mice in . ( c ) Western blot analysis showing the expression level changes of hepatic Pparα in the livers of the mice in . ( d ) Immunohistochemistry analysis showing the expression level changes of hepatic Pparα in the livers of the mice in . ( e ) Western blot analysis showing the expression level changes of hepatic Pparα in the livers of the mice in . ( f ) PIK3R3 was knocked down in HepG2 and LO2 cells by siRNAs transfection. Western blot analysis showing the expression levels of PPARA and PPARG. ( g ) PIK3R3 was overexpressed in hepatic HepG2 and LO2 cells by plasmid transfection. Western blot analysis showing the expression levels of PPARA and PPARG. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Femm2017243'>29350678</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-12276_2018_article_bfemm2017243_fig5_html.jpgAnti-PPAR alpha AntibodyPPARα mediates the effects of PIK3R3 on cellular and hepatic lipid metabolism. ( a ) PIK3R3 was overexpressed in LO2 cells with or without the knockdown of PPARA; western blot analysis showing the protein levels of PIK3R3, PPARA, ACADM and CPT1A. ( b ) PIK3R3 was knocked down in HepG2 cells with or without the overexpression of PPARA; western blot analysis showing the protein levels of PIK3R3, PPARA, ACADM and CPT1A. ( c ) Mice were fed HFD for 8 weeks ( n =6/group) and injected with Ad-control, Ad-Pik3r3 or Ad-Pik3r3+si-Pparα as described in the Materials and methods. Mice were killed for further analysis 5 days after the last injection. Representative H&E, Oil Red O, Pparα, Acadm and Cpt1a stained sections of mouse livers. ( d ) Pik3r3 and Pparα expression levels were detected by western blot. ( e ) Chemical colorimetric diagnostic analysis showing the changes in hepatic TG. ( f ) Chemical colorimetric diagnostic analysis showing changes in serum ketone bodies. Data are expressed as the means±s.e.m. *P <0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Femm2017243'>29350678</a></b>https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00600-12276_2018_article_bfemm2017243_fig6_html.jpgAnti-PPAR alpha AntibodyPIK3R3 regulation of PPARα expression was dependent on HNF4α. ( a ) PIK3R3 was overexpressed in LO2 cells and knocked down in HepG2 cells; western blots were used to detect the protein levels of PIK3R3 and HNF4α. ( b ) PIK3R3 was overexpressed in LO2 cells with or without HNF4α knockdown; western blot analysis showing the expression levels of PIK3R3, HNF4α and PPARα. ( c ) PIK3R3 was knocked down in LO2 cells with or without HNF4α overexpression; western blot analysis showing the expression levels of PIK3R3, HNF4α and PPARα. ( d ) ChIP assay and quantitative PCR analysis showing the binding activity of HNF4α to the PPARα promoter after the overexpression of PIK3R3. ( e ) ChIP assay and quantitative PCR analysis showing the binding activity of HNF4α to the PPARα promoter after the downregulation of PIK3R3. Data are expressed as the means±s.e.m. *P <0.05. <br><b>Index in PubMed under a CC BY license. PMID: <a href='https://idp.nature.com/authorize?response_type=cookie&client_id=grover&redirect_uri=https%3A%2F%2Fwww.nature.com%2Farticles%2Femm2017243'>29350678</a></b> https://www.bosterbio.com/products/primary-antibodies/loading-control-antibodies/anti-beta-actin-antibody-a01263-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01263-beta-actin-primary-antibodies-wb-testing-2_1.jpgAnti-beta Actin ACTB AntibodyWestern Blot of Rabbit Anti-Beta Actin Antibody. Lane 1: molecular weight. Lane 2: human embryonic kidney 293 . Lane 3: human lung carcinoma A549 . Lane 4: mouse brain . Load: 35 µg per lane. Primary antibody: Beta Actin antibody at 1:1,500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~42 kDa corresponding to beta Actin (arrowhead). Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01263-beta-actin-primary-antibodies-ihc-testing-1_1.jpgAnti-beta Actin ACTB AntibodyImmunohistochemistry of Rabbit Anti-Beta Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01263-beta-actin-primary-antibodies-wb-testing-1.pngAnti-beta Actin ACTB AntibodyWestern blot analysis of Beta Actin using anti-Beta Actin antibody (A01263). <br> Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. <br> Lane 1-12: human HEK293T whole cell lysates, <br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Actin antigen affinity purified polyclonal antibody (A01263) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Azure Biosystems c600 system. A specific band was detected for Beta Actin at approximately 42 kDa. The expected band size for Beta Actin is at 42 kDa. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01263-david_lee_1.pngAnti-beta Actin ACTB AntibodyWestern blot analysis of beta Actin using anti-beta Actin antibody (A01263). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30-35 ug of sample under reducing conditions. <br>Lane 1: human 293T whole cell lysates,<br> Lane 2: human A549 whole cell lysates,<br> Lane 3: mouse brain tissue lysates.<br> After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Actin antigen affinity purified polyclonal antibody (A01263) at 1: 1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for beta Actin at approximately 42 kDa. The expected band size for beta Actin is at 42 kDa. https://www.bosterbio.com/products/primary-antibodies/anti-beta-trcp2-antibody-a05523-boster.html2026-03-24T05:06:19+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a05523-fbxw11-primary-antibodies-wb-testing-1.jpgAnti-Beta TrCP2 FBXW11 AntibodyWestern blot using Boster's affinity purified anti-bTrCP2 antibody shows detection of mouse and human bTrCP2 (arrowhead) in NIH3T3 [lane 1] and 293 [lane 2] whole cell lysates, respectively.  The band appears as a 58 kDa protein, although a 62.1 kDa band is predicted.  The identity of faint higher molecular weight bands is not known.  The primary antibody was used at a 1:200 dilution incubated in 5% BLOTTO overnight at 4°C.  Detection occurred using HRP conjugated Goat-anti-Rabbit IgG diluted 1:20,000 in blocking buffer for 1 h at 4 °C. https://www.bosterbio.com/products/primary-antibodies/anti-kshv-orf57-antibody-a19763-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a19763-orf57-primary-antibodies-wb-testing-1.jpgAnti-KSHV ORF57 AntibodyWestern blot using Boster's affinity purified anti-KSHV ORF57 to detect KSHV ORF57 in HEK293 cells transfected with ORF57 expression vector and ORF57 truncations, or in KSHV infected B-cell line (BCBL-1) treated with or without valproic acid to induce viral replication (arrow).  The membrane was probed with the primary antibody diluted 1:7,500 (left) and 1:1,000 (right). Personal Communication, V. Majerciak, M.Zheng, CCR-NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mesothelin-antibody-m03266-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03266-msln-primary-antibodies-fcm-testing-7.jpgAnti-Mesothelin MSLN Monoclonal AntibodyFlow Cytometry Results of Anti-Mesothelin (MOUSE) Monoclonal Antibody. The green histogram shows NCI-H226 cells and blue histogram shows MCF-7 cells. Both cell lines are stained with a 1:800 dilution Anti-Mesothelin (MOUSE) Monoclonal Antibody. The secondary antibody use was Anti-Mouse IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated at the 1:400 dilution. https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03266-msln-primary-antibodies-wb-testing-2.jpgAnti-Mesothelin MSLN Monoclonal AntibodyWestern Blot of Mouse Anti-Mesothelin Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: HeLa [10µg] + Mesothelin-Fc [0.1µg]. Lane 3: HeLa [10µg] + Mesothelin-Fc [0.05µg]. Lane 4: HeLa [10µg] + Mesothelin-Fc [0.02µg]. Lane 5: HeLa Whole Cell Lysate . Primary Antibody: Anti-Mesothelin at 1µg/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG HRP conjugated at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer 30 mins at RT. Exposure: 15 sec. Predicted MW: 40kDa Mesothelin + Fc region 30kDa. Observed MW: ~70-75kDa.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03266-msln-primary-antibodies-ihc-testing-1.jpgAnti-Mesothelin MSLN Monoclonal AntibodyImmunohistochemistry using Boster's anti-mesothelin antibody to react with two epitopes on mesothelin in PEFF human mesothelioma tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used at 10 µg/mL to label these sections as follows: C, MAb MB; and D, MAb MN followed by goat anti-mouse IgG conjugated to horseradish peroxidase at 25 µg/mL in 1% BSA/PBS for 30 minutes. (magnification, ×200; bar, 50 µm). Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03266-msln-primary-antibodies-wb-testing-3.jpgAnti-Mesothelin MSLN Monoclonal AntibodyWestern blotting using Boster's anti-mesothelin antibodies to detect mesothelin-Fc at 100 ng (lane 1), 25 ng (lane 2), 6 ng (lane 3), 2 ng (lane 4) and 0.4 ng (lane 5). Lane 6 contains 50 ng of CDC25-Fc. Proteins were separated on 4-20% gradient gel by SDS-PAGE followed by transfer to PVDF membrane. Primary antibody was used at 1 µg/ml followed by reaction with ALP goat anti-mouse IgG and BCIP/NBT substrate. Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03266-msln-primary-antibodies-ihc-testing-4.jpgAnti-Mesothelin MSLN Monoclonal AntibodyImmunohistochemistry of Mouse anti-Mesothelin antibody. Tissue: human tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-Mesothelin antibody at 15 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: Mesothelin as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-akt3-fitc-antibody-f00520-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/0/f00520-akt3-primary-antibodies-ihc-testing-2.jpgAnti-AKT3 FITC Monoclonal AntibodyImmunohistochemistry of Mouse Monoclonal anti AKT3 Antibody in Mouse Embryonic Kidney. Tissue: Mouse Liver. Fixation: FFPE buffered formalin 10% conc. Ag Retrieval: Heat, Citrate pH 6.2. Pressure Cooker. Primary antibody: anti-AKT3 at 2ug/ml for 1.5 hour @ room Temp. Secondary Ab: MOUSE ON MOUSE HRP POLYMER 45” RT.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/0/f00520-akt3-primary-antibodies-wb-testing-4.jpgAnti-AKT3 FITC Monoclonal AntibodyWestern Blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/0/f00520-akt3-primary-antibodies-wb-testing-5.jpgAnti-AKT3 FITC Monoclonal AntibodyWestern Blot of Mouse Anti-AKT3 antibody. Lane 1: C2C12. Lane 2: MEF#1. Lane 3: MEF#2. Lane 4: A549. Lane 5: Calu-1. Lane 6: PC3. Lane 7: HepG2. Lane 8: Jurkat. Lane 9: SKOV3. Lane 10: 293T. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/0/f00520-akt3-primary-antibodies-wb-testing-6.jpgAnti-AKT3 FITC Monoclonal AntibodyWestern Blot of Mouse anti-AKT3 antibody. Lane 1: Control. Lane 2: Rapa. Lane 3: T50. Lane 4: T250. Lane 5: Control. Lane 6: Rapa. Lane 7: T50. Lane 8: T250. Lane 9: AKT3 null. Load: 35 µg per lane. Primary antibody: AKT-3 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Anti mouse secondary antibody at 1:20,000 for 1 h at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT3.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/0/f00520-akt3-primary-antibodies-ihc-testing-3.jpgAnti-AKT3 FITC Monoclonal AntibodyImmunohistochemistry of Mouse Anti-AKT3 antibody. Tissue: human prostate carcinoma. A) AKT-3 antibody produced using CELLine, B) AKT-3 antibody produced using roller bottle. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT-3 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 1 h at RT. Localization: AKT3 is nuclear and occasionally cytoplasmic. Staining: AKT3 as precipitated brown signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/f/0/f00520-akt3-primary-antibodies-dot-blot-testing-1.jpgAnti-AKT3 FITC Monoclonal AntibodyDot Blot of Mouse anti-AKT3 Monoclonal Antibody Fluorescein Conjugated. Antigen: His-tagged AKT3. Load: Lane 1 - 100 ng Lane 2 - 33.3 ng Lane 3 - 11.1 ng Lane 4 - 3.70 ng Lane 5 - 1.23 ng. Primary antibody: n/a. Secondary antibody: Mouse anti-AKT3 Monoclonal Antibody Fluorescein Conjugated at 1:1,000 for 60 min at RT. Block: 1 HR at RT. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cyclin-a-antibody-a00700-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00700-ccna2-primary-antibodies-wb-testing-3.jpgAnti-Cyclin A CCNA2 AntibodyWestern Blot of Rabbit anti-Cyclin A antibody shows no difference detected between cell lines. Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 µg per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 60kDa for Cyclin A.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00700-ccna2-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin A CCNA2 AntibodyImmunohistochemistry of Rabbit Anti-Cyclin A antibody. Tissue: human testes tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Cyclin A antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Cyclin A is nuclear and cytoplasmic. Staining: Cyclin A as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p130-rb2-antibody-a02118-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02118-rbl2-primary-antibodies-ihc-testing-1_1.jpgAnti-p130 Rb2 RBL2 AntibodyImmunohistochemical staining of mouse tissue using anti-pRb2/p130 antiserum.  The staining shows the location of pRb2/p130 in developing mouse tissue.  Other detection systems should yield similar results. Sections were cut at 5-7 µm, mounted on glass and dried overnight at 37°C.  All sections were deparaffinized in xylene, rehydrated through a graded alcohol series and washed in phosphate-buffered saline (PBS).  PBS was used for all subsequent washes and for antiserum dilution.  Tissue sections were quenched sequentially in 0.5% hydrogen peroxide and blocked with diluted 10% normal goat anti-rabbit serum.  Slides were incubated at 20° C for 1 h with rabbit anti-pRb2/p130 (1:500) dilution, washed, and then reacted with diluted goat anti-rabbit biotinylated antibody for 30 min.  Slides were then reacted with streptavidin-peroxidase conjugate for 30 min at 20° C.  Diaminobenzidine was used as the final chromogen.  Negative controls for each tissue section were prepared by substituting the primary antiserum with pre-immune serum.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02118-rbl2-primary-antibodies-wb-testing-3_1.jpgAnti-p130 Rb2 RBL2 AntibodyWestern Blot of Rabbit Anti-Rb2 p130 Antibody. Lane 1: HEK 293 pcDNA3. Lane 2: HEK 293 pcDNA3-Rb2wt. Lane 3: HEK 293 pcDNA3-Rb2-PM19. Load: 30 µg per lane. Primary antibody: Anti-Rb2 antibody at 1:250 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 130 kDa for p130/Rb2. https://www.bosterbio.com/products/primary-antibodies/anti-p33-ing1-antibody-a02999-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02999-ing1-primary-antibodies-wb-testing-1.jpgAnti-p33 ING1 AntibodyWestern blot analysis is shown using Boster's Affinity Purified anti-p33 ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This western blot shows reactivity with purified recombinant GST tagged human ING1b (lane 1, ~33kDa) and ING1c protein (lane 2, ~24kDa). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Xiaolan Feng, U. Calgary.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02999-ing1-primary-antibodies-diagram-testing-2.jpgAnti-p33 ING1 AntibodyING1 gene structure https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-p21-waf1-antibody-m00145-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-cdkn1a-primary-antibodies-if-testing-3.jpgAnti-p21 WAF1 CDKN1A Monoclonal AntibodyImmunofluorescence of Mouse anti-p21 antibody. Tissue: human brain. Fixation: free-floating. Antigen retrieval: not required. Primary antibody: anti-human-p21 antibody at 1:250 for 1 h at RT. Co-stained with YFP and Sox2 antibodies. Localization: p21 is nuclear and cytoplasmic. Staining: p21 as precipitated blue with Cy5, YFP as precipitated green with Cy2, and Sox2 as precipitated red with Cy3. z‐stacks from confocal expressed as one composite focal plain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00145-cdkn1a-primary-antibodies-wb-testing-1.jpgAnti-p21 WAF1 CDKN1A Monoclonal AntibodyMab anti-Human p21WAF1 antibody (clone WA-1) is shown to detect human p21 by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p21WAF1 at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:5,000. A single band corresponding to human p21WAF1 is detected at ~21 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p21WAF1. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-esrp-1-2-antibody-m06068-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06068-esrp1-esrp2-primary-antibodies-wb-testing-1.jpgAnti-Esrp-1/2 Monoclonal AntibodyAnti-Esrp-1/2 antibody by western blot shows detection in 293T cell extracts. Lane 1: GFP-transfected. Lane 2: Esrp-1 transfected (arrow). Lane 3: Esrp-2 transfected. Each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. https://www.bosterbio.com/products/primary-antibodies/anti-notch-1-antibody-a00033-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00033-notch1-primary-antibodies-wb-testing-4.jpgAnti-NOTCH 1 AntibodyRabbit anti-Human NOTCH 1 (Cleaved N Terminal) was used at a 1:500 dilution to detect mouse Notch 1 by Western blot. Equivalent amounts of lysates from transiently transfected 293 cells expressing recombinant myc-tagged mouse Notch constructs were electrophoresed and transferred to membrane using standard methods. A reaction with diluted primary antibody was followed by washing; reaction with a 1:10,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122), and color development. Lane M: Mol wt markers. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular domain)-myc. Lane 4: N2 (mouse deleted extracellular domain)-myc. Lane 5: N2 (mouse intracellular domain)-myc. Lane 6: N3 (mouse deleted extracellular domain)-myc. Lane 7: N3 (mouse intracellular domain)-myc. Lane 8: N4 (mouse deleted extracellular domain)-myc. Lane 9: N4 (mouse intracellular domain)-myc. Lane 10: N1 (mouse deleted extracellular domain)(V to G)-myc. Personal communication, Dr. Stacey Huppert.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00033-notch1-primary-antibodies-ihc-testing-1.jpgAnti-NOTCH 1 AntibodyImmunohistochemistry of Rabbit anti-Notch1 antibody. Tissue: Exocrine glands of human pancreas. Fixation: FFPE. Primary antibody: Notch1 antibody at 1:200. Staining: moderate to strong membranous staining and faint to moderate cytoplasmic staining. Islets showed faint staining.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00033-notch1-primary-antibodies-wb-testing-2.jpgAnti-NOTCH 1 AntibodyWestern Blot of Rabbit anti-Notch1 antibody. Lane 1: MCF-7 control lysate. Lane 2: MCF-7 +1 nM 17β-estradiol. Lane 3: MCF-7 + 10 μM gamma secretase inhibitor. Load: 35 µg per lane. Primary antibody: Notch1 antibody at 1:500 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 80 kDa for Notch1.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00033-notch1-primary-antibodies-wb-testing-3.jpgAnti-NOTCH 1 AntibodyDot Blot of Rabbit anti-Notch 1 (Cleaved N Terminal) (Human Specific) Antibody. Antigen: Row 1 - Notch 1 Peptide (Cleaved N Terminal) Row 2 - Notch 1 (Intra) Peptide. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: Rabbit anti-Notch 1 (Cleaved N Terminal) (Human Specific) Antibody at 1:1,000 for 60 min at RT. Secondary antibody: HRP Rabbit Secondary at 1:40,000 for 30 min at RT. Block: 1 HR at RT. https://www.bosterbio.com/products/primary-antibodies/anti-pten-p1-antibody-a08380-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a08380-pten-primary-antibodies-wb-testing-1.jpgAnti-PTEN-P1 PTH2 AntibodyWestern blot using Boster's affinity purified anti-PTEN-P1 antibody shows detection at ~55kDa (arrowhead) of endogenous PTEN-P1 in whole cell lysates from human derived cell lines HeLa [lane 4], HEK293 [lane 5] and MCF7 [lane 6]. Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide. The identity of lower molecular weight bands in lane 4 is unknown. Briefly, each lane contains approximately 35µg of lysate. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-l1-orf2-antibody-a19762-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a19762-orf2-primary-antibodies-wb-testing-1.jpgAnti-L1/ORF2 AntibodyWestern blot using Boster's IgY fraction of anti-L1/ORF2 antibody shows detection of induced bacterially expressed human ORF2 (left lane). No specific band staining is seen in the uninduced lane (right lane). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa corresponds to a human L1/ORF2 EN domain fusion protein (arrowhead). Personal communication, D. Symer, NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-lefty-a-antibody-m06558-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06558-lefty2-primary-antibodies-wb-testing-1.jpgAnti-LEFTY A LEFTY2 Monoclonal AntibodyMab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. Detection occurs after 1.0 µg of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1,000. The antibody may be used to detect endogenous human LEFTY. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/anti-trkct1-antibody-a02502-boster.html2026-03-24T05:06:20+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02502-ntrk3-primary-antibodies-wb-testing-1_1.jpgAnti-TrkCT1 NTRK3 AntibodyWestern blot using Boster's affinity purified anti-TrkCT1 to detect over-expressed TrkCT1 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02502-ntrk3-primary-antibodies-wb-testing-2_1.jpgAnti-TrkCT1 NTRK3 AntibodyMouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6,000 dilution. Personal Communication, V. Coppola, CCR-NCI, Frederick, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a02502-ntrk3-primary-antibodies-wb-testing-3_1.jpgAnti-TrkCT1 NTRK3 AntibodyWestern blot using Boster's affinity purified anti-TrkCT1 to detect endogenous TrkCT1 in mouse cortex lysate (Lane 1). Lane 2 is TrkCT1 knock-out cortex lysate. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution. Personal Communication, V. Coppola, CCRNCI, Frederick, MD. https://www.bosterbio.com/products/primary-antibodies/anti-dnmt3l-antibody-a04214-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04214-dnmt3l-primary-antibodies-wb-testing-2.jpgAnti-DNMT3L AntibodyAffinity Purified Rabbit anti-DNMT3L was used at a 1:1,500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody.  Transfection of U2OS cells (10cm dish) was accomplished using 3 µg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al. (2000).  For western blotting the antibody was used at 0.4µg/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04214-dnmt3l-primary-antibodies-figure-testing-3.jpgAnti-DNMT3L AntibodyThe conserved N-terminal cysteine-rich region, the PHD-like motif, characteristic of the Dnmt3 family is shown. The C-terminal domain is the catalytic methyltransferase domain present in Dnmt3a and Dnmt3b whereas the key catalytic motifs are absent in Dnmt3L. Numbers indicate amino acid residues.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a04214-dnmt3l-primary-antibodies-ihc-testing-1.jpgAnti-DNMT3L AntibodyImmunohistochemistry Rabbit Anti-Human DNMT3L Antibody. Antibody: Rabbit Anti-Human DNMT3L Antibody at 1:500. Antigen Retrieval: HIER pH 6.2 Staining: Nuclear staining of testicular germ cells (Left); Neg Ctrl (Right) Human testis 40X. https://www.bosterbio.com/products/primary-antibodies/anti-myosin-antibody-a06446-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a06446-myl12a-primary-antibodies-wb-testing-1.jpgAnti-Myosin MYL9 AntibodyWestern blot using Boster's anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2). Each lane was loaded with 35 µg of lysate. Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein. After blocking with 5% Normal goat serum and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-mad2l1-antibody-m00785-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00785-mad2l1-primary-antibodies-wb-testing-1.jpgAnti-MAD2L1/Mad2 Monoclonal AntibodyWestern blot using Boster's Mab anti-MAD2L1 antibody shows detection of a band at ~24 kDa (arrowhead) corresponding to MAD2L1 present in a HeLa whole cell lysate . Approximately 75 µg of lysate was separated by 4-20% TG SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200. The membrane was washed and reacted with a 1:5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-ipo-38-antibody-m03051-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m03051-hmgn1-primary-antibodies-ihc-testing-1.jpgAnti-IPO-38 HMGN1 Monoclonal AntibodyImmunohistochemistry of Mouse anti-IPO-38 antibody. Tissue: human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-IPO 38 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: IPO-38 as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cenp-e-antibody-m04553-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m04553-cenpe-primary-antibodies-if-testing-1.jpgAnti-CENP-E Monoclonal AntibodyBoster's monoclonal anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. AlexaFluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green). Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-esrp-1-antibody-m06068-1-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m06068-1-esrp1-primary-antibodies-wb-testing-1.jpgAnti-Esrp-1 Monoclonal AntibodyAnti-ESRP-1 by western blot shows detection of ESRP-1 seen in lane 2. Lane 1: GFP-transfected-293T cell extracts, Lane 2: ESRP-1-transfected-293T cell extracts, Lane 3: ESRP2-transfected 293T cell lysates. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution in PBS-T plus milk, and reacted for 1hr at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-esrp-2-antibody-m08004-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m08004-esrp2-primary-antibodies-wb-testing-1.jpgAnti-Esrp-2 Monoclonal AntibodyAnti-ESRP2 by western blot shows detection of ESRP2 in lane 2. Lane G: GFP-transfected-293T cell extract, Lane 1: ESRP1-transfected 293T cell extract, Lane 2: ESRP2-transfected 293T cell extract. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers. https://www.bosterbio.com/products/primary-antibodies/anti-abcb1-antibody-a00049-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-abcb1-primary-antibodies-ihc-testing-1.jpgAnti-ABCB1/Mdr1 AntibodyIHC using Boster ABCB1 Antibody at 20 ug/ml against FFPE human kidney. Moderate to strong cytoplasmic and membranous staining seen in this example. Similar staining also observed in other tissues including liver, kidney, and small intestine. The antibody showed minimal background staining and worked well in formalin-fixed tissues. Image provided courtesy of Lifespan Biosciences.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00049-abcb1-primary-antibodies-wb-testing-2.jpgAnti-ABCB1/Mdr1 AntibodyWestern blot using Boster's affinity purified anti-ABCB1 antibody shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:600, followed by Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG at 1:10,000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/anti-ncoa3-antibody-a01337-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01337-ncoa3-primary-antibodies-wb-testing-3_1.jpgAnti-NCOA3/Src3 AntibodyWestern blot using Boster's affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kDa, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kDa bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01337-ncoa3-primary-antibodies-ihc-testing-1_1.jpgAnti-NCOA3/Src3 AntibodyImmunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a01337-ncoa3-primary-antibodies-ihc-testing-2_1.jpgAnti-NCOA3/Src3 AntibodyImmunohistochemistry of rabbit anti-NCOA3 antibody. Tissue: skin spleen. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-NCOA3 at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: NCOA-3 as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/anti-ajuba-antibody-a03320-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03320-ajuba-primary-antibodies-wb-testing-1.jpgAnti-Ajuba AntibodyWestern blot using Boster's Affinity Purified anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head).  Lanes correspond to 1) vector only transfection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 µg of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody.  Detection occurred using a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a03320-ajuba-primary-antibodies-wb-testing-3.jpgAnti-Ajuba AntibodyWestern blot using Boster's Affinity Purified anti-Ajuba antibody shows detection of a 57-kDa band consistent with the expected MW for Ajuba (arrowhead).  Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates.  Immunoprecipitation of Ajuba followed by western blotting may result in cleaner background staining.  Approximately 5 µg of each preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Ajuba antibody.  Detection occurred using a 1:5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.  A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.  Personal Communication.  E. Pugacheva, Fox Chase Cancer Center, Philadelphia, PA. https://www.bosterbio.com/products/primary-antibodies/anti-il-29-antibody-a07060-boster.html2026-03-24T05:06:21+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a07060-ifnl1-primary-antibodies-wb-testing-1.jpgAnti-IL-29 IFNL1 AntibodyAnti-human IL-29 antibody in western blot shows detection of recombinant human IL-29 raised in E.coli. Recombinant protein (0.1 µg, 19.9 kDa) was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Boster's, Inc. Anti-Human IL-29. After washing, membrane was probed with secondary antibody Dylight™ 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system. https://www.bosterbio.com/products/primary-antibodies/anti-hice1-antibody-a12994-boster.html2026-03-24T05:06:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/1/a12994-haus8-primary-antibodies-wb-testing-1.jpgAnti-HICE1 HAUS8 AntibodyAnti-HICE1 in Western Blot using Boster Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-trpc6-antibody-m00625-boster.html2026-03-24T05:06:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00625-trpc6-primary-antibodies-elisa-testing-3.jpgAnti-TRPC6 Monoclonal AntibodyELISA Results of Mouse Anti-TRPC6 Antibody. Each well was coated in duplicate with 0.1µg of conjugate. The working dilution is 1:31,000. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugation Stabilizer , Rabbit Anti-Mouse IgG HRP conjugated and TMB substrate .https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00625-trpc6-primary-antibodies-wb-testing-2.jpgAnti-TRPC6 Monoclonal AntibodyWestern Blot of Mouse Anti-TRPC6 Antibody. Lane 1: Opal Prestained Molecular Weight Marker . Lane 2: Mouse Pancreas Tissue Lysate [10µg]. Lane 3: MCF-7 Whole Cell Lysate [10µg]. Lane 4: A431 Whole Cell Lysate [10µg]. Lane 5: Jurkat Whole Cell Lysate [10µg]. Primary Antibody: Anti-TRPC6 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Rabbit Anti-Mouse IgG Peroxidase 1:40000 for 30mins at RT. Blocking Buffer: BlockOut Buffer for 30mins at RT. Predicted MW: ~30kDa. Observed MW: ~40kDa. Exposure: 5sec.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00625-trpc6-primary-antibodies-ihc-testing-1.jpgAnti-TRPC6 Monoclonal AntibodyImmunohistochemistry using Boster's anti-TRPC6 monoclonal antibody shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 µg/mL. The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m00625-trpc6-primary-antibodies-wb-testing-4.jpgAnti-TRPC6 Monoclonal AntibodyWestern Blot of Mouse anti-TRPC6 Antibody. Lane 1: Mouse Kidney WCL . Load: 10 µg per lane. Primary antibody: TRPC6 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: donkey anti-mouse DyLight™ 649 at 1:20,000 for 30 min at RT. Block: 30 min at RT. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-timp4-antibody-m07131-boster.html2026-03-24T05:06:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07131-timp4-primary-antibodies-ihc-testing-1.jpgAnti-TIMP4 Monoclonal AntibodyImmunohistochemistry of Mouse Anti-TIMP4 Antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: a) isotype specific IgG2bk monoclonal; b) monoclonal TIMP-4 antibody (clone 9:4-7); and c) polyclonal TIMP-4, each at 10 µg/mL. Secondary antibody: Peroxidase secondary antibody at 1:10,000 for 45 min at RT. Localization: TIMP-4 is secreted. Staining: TIMP-4 as precipitated brown signal.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07131-timp4-primary-antibodies-ihc-testing-2.jpgAnti-TIMP4 Monoclonal AntibodyImmunohistochemistry of Mouse anti-TIMP4 antibody. Tissue: human small intestine. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/m/0/m07131-timp4-primary-antibodies-ihc-testing-3.jpgAnti-TIMP4 Monoclonal AntibodyImmunohistochemistry of Mouse anti-TMIP4 antibody. Tissue: human kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: anti-TIMP4 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Staining: TIMP4 as precipitated red signal with hematoxylin purple nuclear counterstain. https://www.bosterbio.com/products/primary-antibodies/anti-il-6-antibody-a00102-1-boster.html2026-03-24T05:06:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00102-1-il6-primary-antibodies-ihc-testing-2.jpgAnti-IL-6 AntibodyImmunohistochemistry of Rabbit Anti-IL6-Antibody. Tissue: human lymph nodes at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-IL-6 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: IL-6 is cytoplasmic. Staining: IL-6 as precipitated brown signal (B 20x, B 40x) hematoxylin purple nuclear counterstain. Negative Control (A 20x).https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00102-1-il6-primary-antibodies-wb-testing-1.jpgAnti-IL-6 AntibodyWestern blot using Boster's anti-Human IL6 antibody shows detection of a band ~24 kDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1:500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG secondary antibody diluted 1:40,000 in blocking buffer and FemtoMax™ Chemiluminescent substrate . Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad). https://www.bosterbio.com/products/primary-antibodies/anti-cdk2-antibody-a00166-boster.html2026-03-24T05:06:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00166-cdk2-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin-dependent kinase 2 cdk2 AntibodyImmunohistochemistry of Anti-CDK2 antibody. Tissue: human skin formalin fixed and paraffin embedded. No pre-treatment of sample was required. Primary Antibody: Anti-CDK2 was diluted 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00166-cdk2-primary-antibodies-wb-testing-2.jpgAnti-Cyclin-dependent kinase 2 cdk2 AntibodyRabbit anti-cdk2 was used at a 1:200 dilution to detect cdk-2 in asynchronous HeLa cell lysates (run in duplicate). Approximately 50 µg of lysate was separated on a 15% SDS-PAGE gel. Cdk-2 is indicated by an arrowhead as a 32-33 kDa band. Note that multiple isoforms as well as phosphorylated forms of cdk-2 may be detected by this antibody. Primary antibody was reacted at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (611-103-122) preceded color development. https://www.bosterbio.com/products/primary-antibodies/anti-il-8-antibody-a00423-boster.html2026-03-24T05:06:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00423-il8-primary-antibodies-wb-testing-1.jpgAnti-IL-8 CXCL8 AntibodyWestern Blot of Rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1,000 overnight at 4°C. Secondary antibody: HRP Goat-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8. https://www.bosterbio.com/products/primary-antibodies/monoclonal-primary-antibodies/anti-cdk5-antibody-a00511-boster.html2026-03-24T05:06:22+00:00daily0.9https://www.bosterbio.com/pub/media/catalog/product/cache/ac8b64ee610939d0b5d6545d1a7fa448/a/0/a00511-cdk5-primary-antibodies-ihc-testing-1.jpgAnti-Cyclin-dependent-like kinase 5 Cdk5 AntibodyBoster's anti-CDK5 antibody was diluted 1:500 to detect CDK5 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.